PMC:4236617 / 51279-52678 JSONTXT

{"project":"MicrobeTaxon","denotations":[{"id":"T284","span":{"begin":16,"end":21},"obj":"9823"},{"id":"T285","span":{"begin":1316,"end":1321},"obj":"9823"},{"id":"T286","span":{"begin":33,"end":46},"obj":"1604"},{"id":"T287","span":{"begin":125,"end":132},"obj":"9823"},{"id":"T288","span":{"begin":228,"end":235},"obj":"9823"},{"id":"T289","span":{"begin":849,"end":854},"obj":"9606"},{"id":"T290","span":{"begin":338,"end":342},"obj":"562"},{"id":"T291","span":{"begin":573,"end":577},"obj":"562"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Conclusions\nThe swine intestinal L. amylovorus strains investigated in this study varied in their abilities to adhere to the porcine small intestinal epithelial cell line IPEC-1, while none of the strains adhered efficiently to porcine gastric or intestinal mucus. Several of the strains markedly inhibited the adherence of F4-fimbriated ETEC to IPEC-1 cells, and all inhibited the growth of various intestinal pathogens in vitro. The abilities of the strains to adhere to IPEC-1 cells were often associated with, but were not necessary for, the exclusion of F4-fimbriated ETEC from these cells, suggesting that additional mechanisms, other than competitive binding, were involved in the inhibition. The major S-layer proteins of the strains alone did not mediate the adherence of the strains to IPEC-1 cells. The immunological responses induced in human dendritic cells by the strains were of varying intensity and of a mixed type with both pro- and anti-inflammatory cytokines induced, with the same strain being the most potent inducer of both types of cytokines. The results indicate that while some commensals show adhesive capacity to epithelial cells, many may co-exist and benefit the host by mechanisms not attributable to adhesion to epithelial cells or mucus. The results warrant further studies of these swine intestinal strains if they are to be developed as probiotic feed supplements."}