PMC:4236617 / 24277-25571 JSONTXT

{"project":"2_test","denotations":[{"id":"25070625-18029488-12189978","span":{"begin":332,"end":334},"obj":"18029488"}],"text":"Adherence of L. amylovorus strains to IPEC-1 cells\nIn contrast to mucus binding, clear differences were observed in the adherence of the L. amylovorus strains to porcine small intestinal epithelial cells as represented by the cell line IPEC-1 (Figure 2A). The previously reported binding of L. amylovorus DSM 16698 to IPEC-1 cells [26] was confirmed, and the adherence of the strains GRL 1112 and GRL 1115 was found to be within a similar range, though the three strains displayed high variabilities in different experiments. In contrast, the type strain of L. amylovorus, DSM 20531T, isolated from silage, and the rest of the porcine intestinal isolates were clearly less adhesive. An example of the dose–response of binding is shown in Figure 2B.\nFigure 2 Adherence of L. amylovorus strains to IPEC-1 cells. A) The adherence of 3H-labeled L. amylovorus strains (A600nm = 0.25) to IPEC-1 cells grown on Thincert™ wells showing the means and standard deviations of 3–7 independent experiments, each with three technical replicates. B) An example of the adherence of L. amylovorus DSM 16698, GRL 1112, GRL 1115 and GRL 1118 to IPEC-1 cells as the function of cell density. The means of three technical replicates from one representative experiment are shown. Dpm, disintegrations per minute.\n\nI"}

{"project":"MicrobeTaxon","denotations":[{"id":"T131","span":{"begin":13,"end":26},"obj":"1604"},{"id":"T132","span":{"begin":137,"end":150},"obj":"1604"},{"id":"T133","span":{"begin":162,"end":169},"obj":"9823"},{"id":"T134","span":{"begin":627,"end":634},"obj":"9823"},{"id":"T135","span":{"begin":291,"end":314},"obj":"1604"},{"id":"T136","span":{"begin":558,"end":571},"obj":"1604"},{"id":"T137","span":{"begin":573,"end":583},"obj":"1423723"},{"id":"T138","span":{"begin":291,"end":314},"obj":"695563"},{"id":"T297","span":{"begin":772,"end":785},"obj":"1604"},{"id":"T298","span":{"begin":842,"end":855},"obj":"1604"},{"id":"T299","span":{"begin":1067,"end":1090},"obj":"1604"},{"id":"T300","span":{"begin":1067,"end":1090},"obj":"695563"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Adherence of L. amylovorus strains to IPEC-1 cells\nIn contrast to mucus binding, clear differences were observed in the adherence of the L. amylovorus strains to porcine small intestinal epithelial cells as represented by the cell line IPEC-1 (Figure 2A). The previously reported binding of L. amylovorus DSM 16698 to IPEC-1 cells [26] was confirmed, and the adherence of the strains GRL 1112 and GRL 1115 was found to be within a similar range, though the three strains displayed high variabilities in different experiments. In contrast, the type strain of L. amylovorus, DSM 20531T, isolated from silage, and the rest of the porcine intestinal isolates were clearly less adhesive. An example of the dose–response of binding is shown in Figure 2B.\nFigure 2 Adherence of L. amylovorus strains to IPEC-1 cells. A) The adherence of 3H-labeled L. amylovorus strains (A600nm = 0.25) to IPEC-1 cells grown on Thincert™ wells showing the means and standard deviations of 3–7 independent experiments, each with three technical replicates. B) An example of the adherence of L. amylovorus DSM 16698, GRL 1112, GRL 1115 and GRL 1118 to IPEC-1 cells as the function of cell density. The means of three technical replicates from one representative experiment are shown. Dpm, disintegrations per minute.\n\nI"}