PMC:4236617 / 19248-20203 JSONTXT

{"project":"MicrobeTaxon","denotations":[{"id":"T114","span":{"begin":15,"end":20},"obj":"9606"},{"id":"T115","span":{"begin":235,"end":240},"obj":"9606"},{"id":"T116","span":{"begin":53,"end":66},"obj":"1604"},{"id":"T117","span":{"begin":158,"end":167},"obj":"2"},{"id":"T118","span":{"begin":205,"end":214},"obj":"2"},{"id":"T119","span":{"begin":389,"end":397},"obj":"2"},{"id":"T120","span":{"begin":655,"end":660},"obj":"9606"},{"id":"T121","span":{"begin":795,"end":800},"obj":"9606"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Stimulation of human moDCs and cytokine measurements\nL. amylovorus strains were cultivated overnight, collected and washed with PBS. The A600nm values of the bacterial suspensions were normalized, and the bacterial cells were added to human moDCs at the multiplicity of infection (MOI) 1, 10, and 100 in RPMI 1640 containing FCS, HEPES, antibiotics, and glutamine. The same medium without bacteria was used as a control. After 24 h, cell culture supernatants were collected and stored at -20°C before further analyses. The supernatants were analyzed with the Bio-Rad’s Bio-Plex Pro Cytokine assay using the Bio-Plex -200 platform (Bio-Rad, Hercules, CA). Human TNF-α, IL-1β, IL-6, IL-10, and IL-12 quantification was performed for undiluted samples according to the manufacturer’s instructions. Human IP-10/CXCL10 was measured separately with the OptEIA ELISA kit (BD, Franklin Lakes, New Jersey) using samples diluted with sample matrix RPMI 1640 medium."}