PMC:4197335 / 34181-34605 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/4197335","sourcedb":"PMC","sourceid":"4197335","source_url":"http://www.ncbi.nlm.nih.gov/pmc/4197335","text":"Biochemical Assays\nQuantitative RT-PCR (qRT-PCR) assays were carried out using the TaqMan Gene Expression Assays kit (Applied Biosystems). The kinase profiling of DTP3 across 142 human kinases was outsourced. Western blots, coimmunoprecipitations, and kinase assays were performed as described previously (Papa et al., 2004, 2008; Mauro et al., 2011). Additional details are provided in Supplemental Experimental Procedures.","divisions":[{"label":"Title","span":{"begin":0,"end":18}}],"tracks":[{"project":"2_test","denotations":[{"id":"25314077-14743220-17318434","span":{"begin":345,"end":349},"obj":"14743220"},{"id":"25314077-18382767-17318434","span":{"begin":345,"end":349},"obj":"18382767"},{"id":"25314077-21968997-17318434","span":{"begin":345,"end":349},"obj":"21968997"}],"attributes":[{"subj":"25314077-14743220-17318434","pred":"source","obj":"2_test"},{"subj":"25314077-18382767-17318434","pred":"source","obj":"2_test"},{"subj":"25314077-21968997-17318434","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec9493","default":true}]}]}}