PMC:4163918 / 24222-24547 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4163918","sourcedb":"PMC","sourceid":"4163918","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4163918","text":"Reconstituted inflammasome in a cell-free system\nRecombinant NLRP3, ASC and pro-caspase-1 were incubated with recombinant PKR and ATP (2.5mM) /Poly I:C (0.001 μg/mL) in the PKR reaction buffer (20 mM HEPES [pH7.4], 10 mM MgCl2, 40 mM KCl, 2 mM DTT) at 37 °C for 1 h. Caspase-1 activity was measured by hydrolysis of WEHD-pNA.","divisions":[{"label":"Title","span":{"begin":0,"end":48}}],"tracks":[]}