PMC:4163918 / 23246-23684 JSONTXT

{"project":"2_test","denotations":[{"id":"22801494-16407889-75130332","span":{"begin":126,"end":128},"obj":"16407889"},{"id":"22801494-16407889-75130332","span":{"begin":126,"end":128},"obj":"16407889"},{"id":"22801494-20547845-75130333","span":{"begin":182,"end":183},"obj":"20547845"},{"id":"22801494-20547845-75130333","span":{"begin":182,"end":183},"obj":"20547845"}],"text":"Western-blot\nProteins from cell-free supernatants were extracted by methanol/chloroform precipitation as described previously 18. Cell extracts were prepared as described previously 7. Samples were separated by 4-20% SDS-PAGE or 4-20% native-PAGE and were transferred onto PVDF membranes. The relative levels of HMGB1 in the culture medium were determined by Western blot analysis using recombinant HMGB1 protein to make a standard curve."}