PMC:4162895 / 6924-9799 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4162895","sourcedb":"PMC","sourceid":"4162895","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4162895","text":"ISSR fingerprinting of 19 primers generated 270 scorable fragments, out of which 252 were polymorphic (93.33 % polymorphism) (Table 1). The primers viz., UBC 808, 810, 840, 841, 855, 862, 881 and 900 exhibited 100 percent polymorphism. The number of polymorphic fragments for each primer varied from 7 to 19 with an average of 13.26 polymorphic fragments (Fig. 1). ISSR banding pattern exhibited a maximum of 133 fragments in S. japonica followed by C. pareira var. hirsuta (132 fragments) and C. peltata (119). A maximum of eight unique bands were observed in C. pareira var. hirsuta while screening with the primer UBC 851. Interestingly, the number of unique bands in C. peltata was less compared to other plants. But in S. japonica, six and seven unique bands were observed while screening with the primers UBC 890 and UBC 900, respectively. Here also, the total number of unique bands was highest in C. pareira var. hirsuta (Table 2).\nTable 1 Total number of bands and percentage of polymorphism amplified by 19 ISSR primers\nSl. no. Primer Annealing temperature Total number of bands Number of polymorphic bands Number of monomorphic bands Polymorphism (%)\n1 (ACTG)4 49 11 10 1 90.91\n2 UBC 807 50 9 8 1 88.89\n3 UBC 808 52 17 17 0 100\n4 UBC 810 50 16 16 0 100\n5 UBC 825 50 14 13 1 92.86\n6 UBC 840 54 17 17 0 100\n7 UBC 841 54 12 12 0 100\n8 UBC 842 54 10 8 2 80\n9 UBC 847 52 16 13 3 81.25\n10 UBC 851 54 16 14 2 87.5\n11 UBC 854 56 13 12 1 92.31\n12 UBC 855 52 19 19 0 100\n13 UBC 857 54 15 14 1 93.33\n14 UBC 859 54 8 7 1 87.50\n15 UBC 862 55 13 13 0 100\n16 UBC 873 52 12 9 3 75\n17 UBC 881 59 19 19 0 100\n18 UBC 890 59 20 18 2 90\n19 UBC 900 59 13 13 0 100\nTotal 270 252 18 93.33\nFig. 1 Representative ISSR profiles of Patha plants using primers a (ACTG)4, b UBC 840, c UBC 847, d UBC 857, e UBC 881 and f UBC 890. M represents 1 kb ladder, lane 1Cissampelos pareira var. hirsuta, lane 2Cyclea peltata and lane 3Stephania japonica\nTable 2 Primerwise ISSR banding pattern of Patha plants\nPrimer Cissampelos pareira var. hirsuta Cyclea peltata Stephania japonica\nTB PB MB UB TB PB MB UB TB PB MB UB\n(ACTG)4 6 5 1 3 4 3 1 1 6 5 1 3\nUBC 807 4 3 1 0 7 6 1 4 3 2 1 1\nUBC 808 9 9 0 5 4 4 0 1 9 9 0 5\nUBC 810 7 7 0 4 10 10 0 5 6 6 0 0\nUBC 825 9 8 1 6 6 5 1 1 6 5 1 1\nUBC 840 9 9 0 3 10 10 0 3 7 7 0 2\nUBC 841 3 3 0 2 4 4 0 4 6 6 0 5\nUBC 842 4 2 2 0 7 5 2 2 7 5 2 2\nUBC 847 9 6 3 5 7 4 3 2 9 6 3 3\nUBC 851 11 9 2 8 7 5 2 1 6 4 2 1\nUBC 854 5 4 1 2 7 6 1 3 6 5 1 4\nUBC 855 9 9 0 7 6 6 0 1 10 10 0 5\nUBC 857 9 8 1 5 7 6 1 1 8 7 1 1\nUBC 859 4 3 1 3 4 3 1 1 4 3 1 1\nUBC 862 6 6 0 4 6 6 0 3 5 5 0 2\nUBC 873 7 4 3 4 5 2 3 2 6 3 3 3\nUBC 881 9 9 0 6 8 8 0 3 9 9 0 3\nUBC 890 9 7 2 5 7 5 2 3 12 10 2 6\nUBC 900 3 3 0 3 3 3 0 2 8 8 0 7\nTotal 132 114 18 75 119 101 18 43 133 115 18 55\nTB number of total bands, PB number of polymorphic bands, MB number of monomorphic bands, UB number of unique 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