PMC:4158115 / 6243-8068 JSONTXT

{"project":"MicrobeTaxon","denotations":[{"id":"T57","span":{"begin":683,"end":692},"obj":"2"},{"id":"T58","span":{"begin":772,"end":781},"obj":"2"},{"id":"T59","span":{"begin":1282,"end":1291},"obj":"2"},{"id":"T60","span":{"begin":32,"end":43},"obj":"199"},{"id":"T61","span":{"begin":463,"end":470},"obj":"9606"},{"id":"T62","span":{"begin":1516,"end":1525},"obj":"2"},{"id":"T63","span":{"begin":140,"end":151},"obj":"199"},{"id":"T64","span":{"begin":230,"end":241},"obj":"199"},{"id":"T65","span":{"begin":580,"end":591},"obj":"199"},{"id":"T66","span":{"begin":938,"end":949},"obj":"199"},{"id":"T67","span":{"begin":1040,"end":1051},"obj":"199"},{"id":"T68","span":{"begin":1306,"end":1317},"obj":"199"},{"id":"T69","span":{"begin":1567,"end":1578},"obj":"199"},{"id":"T70","span":{"begin":347,"end":355},"obj":"9606"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"2.3. Quantitative Comparison of C. concisus Growth under AnaeroH2− and AnaeroH2+ Conditions\nTo further quantitatively compare the growth of C. concisus under AnaeroH2− and AnaeroH2+ conditions, the colony forming unit (CFU) of 12 C. concisus strains grown under these two conditions was determined. These 12 strains included six oral strains from patients with CD, three oral strains from UC, two oral strains from healthy controls, and one enteric strain from a patient with UC. These 12 strains were randomly selected from the 63 strains used in the experiments in Section 2.2.\nC. concisus strains were first cultured on HBA plates under Oricon condition at 37°C for 48 hours. The bacterial cells were collected and washed once with phosphate buffered saline (PBS). The bacterial pellet of each strain was resuspended in PBS and OD600 was adjusted to 0.05, which was used as the initial inoculum for further assessment of the growth of C. concisus under AnaeroH2− and AnaeroH2+ conditions.\nThe initial inoculum suspension (50 μL) of each C. concisus strain was inoculated onto six HBA plates using a sterile L-shaped glass rod. Three plates were incubated under AnaeroH2− condition and the remaining three plates were incubated under AnaeroH2+ condition for 48 hours at 37°C.\nThe bacterial cells of each C. concisus strain collected from the three plates incubated under AnaeroH2− condition and the three plates incubated under AnaeroH2+ condition were pooled, respectively (1 mL of PBS was used for collection of bacterial cells from each plate). From each pooled C. concisus suspension, serial dilutions (1 : 10 to 1 : 108) were prepared. Each of the dilutions (5 μL) was inoculated onto HBA plates in triplicate. The plates were further incubated under Oricon condition for 48 hours at 37°C to determine the CFU numbers."}