PMC:4157146 / 59874-60738 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4157146","sourcedb":"PMC","sourceid":"4157146","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4157146","text":"CCDC151 Is Localized to Respiratory Ciliary Axonemes\n(A) Immunoblot analysis (right lane) of protein lysates (left lane) from human respiratory cells compared to protein standard show that CCDC151 antibodies specifically detect a single protein band corresponding to the predicted size (∼64 kDa).\n(B) Respiratory epithelial cells obtained by nasal biopsy of unaffected individuals and PCD-affected individuals carrying CCDC151 mutations were double labeled with antibodies directed against acetylated α-tubulin (green) and CCDC151 (red). Nuclei were stained with Hoechst 33342 (blue). Both proteins colocalize (yellow) along the ciliary axonemes in cells from the healthy controls, while in respiratory cells of OP-675 and OP-1255 carrying CCDC151 recessive loss-of-function mutations, CCDC151 is not detectable in the ciliary axonemes. Scale bars represent 10 μm.","divisions":[{"label":"p","span":{"begin":0,"end":52}},{"label":"p","span":{"begin":53,"end":296}}],"tracks":[]}