PMC:4157146 / 38495-40005 JSONTXT

{"project":"2_test","denotations":[{"id":"25192045-23261303-2045897","span":{"begin":327,"end":329},"obj":"23261303"},{"id":"25192045-23849778-2045898","span":{"begin":726,"end":728},"obj":"23849778"},{"id":"25192045-23261303-2045899","span":{"begin":1089,"end":1091},"obj":"23261303"}],"text":"CCDC151 Plays a Role in Assembly of the Outer Dynein Arm Docking Complex in Addition to Its Role in Outer Dynein Arm Assembly\nTo further understand the functional role of CCDC151 in ODA assembly, we also studied the localization of CCDC114, which is an ODA-DC subunit responsible for axonemal microtubule attachment of the ODAs16 in CCDC151 mutant cilia. We found that CCDC114 was undetectable in the respiratory cilia of CCDC151-mutant individuals compared to the normal axonemal localization of CCDC114 in respiratory cilia from unaffected controls (Figure 6A). This suggests that the axonemal localization of CCDC114 is CCDC151 dependent. Since the localization of the ODA-DC-related ARMC4 is known to be CCDC114 dependent,21 we also studied ARMC4 localization in CCDC151 mutant cilia. ARMC4 was also undetectable in the respiratory cilia of CCDC151 mutant individuals, indicating that similarly to CCDC114, the axonemal localization of ARMC4 is CCDC151 dependent (Figure 6B).\nConsidering the similarities in phenotype caused by CCDC114 and CCDC151 mutations with regard to ODA defects,16 we tested for possible interactions between these proteins. Using myc- and FLAG-tagged proteins that were coexpressed in HEK293 cells, we found by coimmunoprecipitation that CCDC151 interacted with CCDC114 (Figure 6C), but not DNAI1, DNAI2, and DNAL1, whose mutations also cause ODA defects (data not shown). We confirmed the reciprocal interaction between CCDC114 and CCDC151 by yeast two-hybrid analysis (Figure S6)."}