PMC:4137170 / 8207-9033 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4137170","sourcedb":"PMC","sourceid":"4137170","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4137170","text":"Carrier preparation\nConformational change of the rhBMP-2 due to sterilization was avoided by mixing the rhBMP-2 solution with a powder gel composite to a final concentration of 1 mg/ml rhBMP-2. The in situ mixing process is a method used to generate an rhBMP-2 loaded injectable carrier by mixing the powder gel and the porous spherical particles for loading rhBMP-2.\nIn other words, one syringe contained porous β-TCP microspheres and the other syringe held the HA-based powder-gel containing 1 mg/ml rhBMP-2, and the relative weight ratio of the materials was 1:9. These two syringes were interconnected through a 2-way connector and 30 cycles of piston movement of each syringe were performed to ensure that the mixture was homogeneous. Collectively, β-TCP microspheres and HA were used as an rhBMP-2 carrier in this study.","divisions":[{"label":"title","span":{"begin":0,"end":19}},{"label":"p","span":{"begin":20,"end":367}}],"tracks":[]}