PMC:4137170 / 7372-8205 JSONTXT

{"project":"2_test","denotations":[{"id":"24928668-19993-61779598","span":{"begin":559,"end":561},"obj":"19993"}],"text":"The powder gel was prepared to facilitate the loading of rhBMP-2 and to improve the fixation force of the carrier at the site of a bone defect by absorbing the blood and body fluids generated at the defect site. The powder gel was a cross-linked hyaluronic acid-based hydrogel consisting of HA (Bioland Co., Ochang, South Korea) with a high molecular weight of 3 million Daltons. This HA-based hydrogel was prepared through cross-linking by adding butanediol diglycidyl ether (Sigma-Aldrich, St. Louis, USA), a cross-linking agent, to 2.7 wt% of HA solution [17]. The remaining reagents were removed without resuspending by dialysis against 1X PBS (Sigma-Aldrich, USA) for 5 days. The resulting HA-based hydrogel was lyophilized for 4 days and then ground and sieved to yield a gel powder with particles less than 100 µm in diameter."}