PMC:4137170 / 14207-15544 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4137170","sourcedb":"PMC","sourceid":"4137170","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4137170","text":"Histologic evaluation\nThe full length of the tibia specimen was fixed in formalin for 5 days and the tibiae containing each specimen were divided into two parts. Gross section tissue was put into a cassette and washed for 6 h and then dehydrated in 100 % alcohol. The tissue was put into methacrylate-based chemical curing resin and stirred for 2 days, and then it was stirred and embedded by dissolving in benzoyl peroxide. The block was trimmed and sectioned along the longitudinal axis of the dental implant fixture using an EXAKT cutting instrument (BS-3000 N). Moreover, a 4 μm section was made at the right center of the implant along the sagittal plane and included the surrounding tibia. Grinding was carried out using an EXAKT grinding machine (4,110), and an acrylic slide attachment was performed. The slide was stained with hematoxylin and eosin (H\u0026E) staining and viewed with a light microscope to determine the bond between the bone and the implant and the new bone formation around the implant. Histomorphometric evaluations of the dental implant fixtures were carried out after a scaled calibration using a morphometry program (LEICA IM50 Image Manager, version 4.0). The bone-to-implant contact ratio was measured in the marrow space of the tibia. All measurements were performed using a (X12.5) magnification objective.","divisions":[{"label":"title","span":{"begin":0,"end":21}}],"tracks":[]}