PMC:4136538 / 11633-12220 JSONTXT

{"project":"2_test","denotations":[{"id":"25143871-19750482-63946022","span":{"begin":234,"end":236},"obj":"19750482"},{"id":"25143871-19750482-63946022","span":{"begin":234,"end":236},"obj":"19750482"},{"id":"T98268","span":{"begin":234,"end":236},"obj":"19750482"},{"id":"T90479","span":{"begin":234,"end":236},"obj":"19750482"}],"text":"Spleens and lumbar spinal cords were collected following transcardiac perfusion and processed for the assessment of IFN-γ levels via enzyme-linked immunosorbent assay (ELISA). Tissue homogenates were prepared as described previously [13] and stored at −80°C until analysis. Levels of IFN-γ were determined with a Duo Set ELISA kit (R\u0026D Systems, Minneapolis, MN) following the manufacturers’ protocol. The IFN-γ level of each sample was normalized based on the protein concentration of the particular sample as determined by the BCA protein assay (Pierce-Thermo Scientific, Rockford, IL)."}