PMC:4136538 / 11593-12220 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4136538","sourcedb":"PMC","sourceid":"4136538","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4136538","text":"Determination of tissue levels of IFN-γ\nSpleens and lumbar spinal cords were collected following transcardiac perfusion and processed for the assessment of IFN-γ levels via enzyme-linked immunosorbent assay (ELISA). Tissue homogenates were prepared as described previously [13] and stored at −80°C until analysis. Levels of IFN-γ were determined with a Duo Set ELISA kit (R\u0026D Systems, Minneapolis, MN) following the manufacturers’ protocol. The IFN-γ level of each sample was normalized based on the protein concentration of the particular sample as determined by the BCA protein assay (Pierce-Thermo Scientific, Rockford, IL).","divisions":[{"label":"title","span":{"begin":0,"end":39}}],"tracks":[{"project":"2_test","denotations":[{"id":"25143871-19750482-63946022","span":{"begin":274,"end":276},"obj":"19750482"},{"id":"25143871-19750482-63946022","span":{"begin":274,"end":276},"obj":"19750482"},{"id":"T98268","span":{"begin":274,"end":276},"obj":"19750482"},{"id":"T90479","span":{"begin":274,"end":276},"obj":"19750482"}],"attributes":[{"subj":"25143871-19750482-63946022","pred":"source","obj":"2_test"},{"subj":"25143871-19750482-63946022","pred":"source","obj":"2_test"},{"subj":"T98268","pred":"source","obj":"2_test"},{"subj":"T90479","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#cf93ec","default":true}]}]}}