PMC:4135037 / 33061-34196 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4135037","sourcedb":"PMC","sourceid":"4135037","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4135037","text":"Cdk4 knockdown in insulinoma cells results in delayed S phase entry with diminished RB phosphorylation, while Cdk2 knockdown shows minimum effects. (A) Expression of cell cycle proteins and phosphorylation of RB and p130 in INS-1 insulinoma cells transfected with Cdk2 or Cdk4 siRNA. Cells at 24 h post transfection (C, control dsRNA; K2, Cdk2 siRNA; K4, Cdk4 siRNA) were cultured in low (0.1 mM) glucose for 48 h, followed by incubation in high (11 mM) glucose for the indicated times. Immunoblotting was performed using the indicated antibodies. (B) Cell cycle profiles of INS-1 cells transfected with control dsRNA (white columns), Cdk2 siRNA (grey columns) and Cdk4 siRNA (black columns) determined by flow cytometry with assessment of BrdU incorporation. Data are representative of three independent experiments. (C) Effects of Cdk4 silencing on RB phosphorylation in Min6 insulinoma cells. Cells were transfected with control dsRNA (C) or Cdk4 siRNA (K4), incubated with low glucose from 24 to 72 h posttransfection, and then treated with high glucose for 24 more hrs. Immunoblotting was performed using the indicated antibodies.","tracks":[]}