PMC:4129400 / 15222-16407 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4129400","sourcedb":"PMC","sourceid":"4129400","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4129400","text":"To validate that the antibodies generated against epitopes within each protein were targeting the protein of interest, we performed two analyses. First, for the 57 pairs of antibodies directed at different epitopes for the same protein that passed our screen, we tested for correlated measurements between interindividual protein levels measured by both antibodies. We observed that 44 of the 57 had correlated measurements (ρ \u003e 0). Discordance between multiple antibodies to the same protein could be because of technical variation or differential isoform levels, because each epitope is directed to a unique region of each protein. Second, approximately 50 amino acids surrounding known antibody epitopes (because exact epitope information was proprietary) were remapped to the human genome (UCSC Genome Browser build hg18) using BLAT31 and epitopes that contained at least one nonsynonymous SNP from dbSNP (release 132)32 or matched multiple regions in the genome with at least 95% identity were flagged but retained, because the proprietary nature of epitope disclosure prevented us from knowing which ∼5–8 amino acid fragment of the 50 amino acids was used to create the antibody.","tracks":[{"project":"2_test","denotations":[{"id":"25087611-11932250-2046321","span":{"begin":836,"end":838},"obj":"11932250"},{"id":"25087611-11125122-2046322","span":{"begin":922,"end":924},"obj":"11125122"}],"attributes":[{"subj":"25087611-11932250-2046321","pred":"source","obj":"2_test"},{"subj":"25087611-11125122-2046322","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#d8ec93","default":true}]}]}}