PMC:4103454 / 25023-25476 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4103454","sourcedb":"PMC","sourceid":"4103454","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4103454","text":"Gel Mobility Shift Assays\nVarying amounts of purified recombinant protein were incubated with 1 nM radiolabelled substrate at 20°C for 30 min in binding buffer (Supplemental Information). Following addition of 2.5% Ficoll-400, samples were run on 8% native polyacrylamide gels in TBE buffer. Gels were dried, exposed to storage phosphor screens, quantified by a Typhoon FLA 9500 (GE Healthcare) phosphorimager, and analyzed with the ImageQuant software.","divisions":[{"label":"Title","span":{"begin":0,"end":25}}],"tracks":[]}