PMC:4103454 / 24243-25021 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/4103454","sourcedb":"PMC","sourceid":"4103454","source_url":"https://www.ncbi.nlm.nih.gov/pmc/4103454","text":"Activity Assays\nDNA substrates were prepared as in MacKay et al. (2010) with the exception of Jbm5, prepared as in Kvaratskhelia et al. (2001). Purified recombinant proteins were preincubated with the radiolabelled substrate in binding buffer (Supplemental Information) at the reaction temperature for 10 min to allow binding to occur. The reaction was started by the addition of divalent metal ions and stopped by mixing with a 2-fold excess of EDTA. After addition of 66% formamide, the samples were heat denatured and analyzed by denaturing PAGE (12% polyacrylamide and 8 M urea in Tris-borate-EDTA [TBE] buffer). Gels were dried, exposed to storage phosphor screens, quantified by a Typhoon FLA 9500 (GE Healthcare) phosphorimager, and analyzed with the ImageQuant software.","divisions":[{"label":"Title","span":{"begin":0,"end":15}}],"tracks":[{"project":"2_test","denotations":[{"id":"24981866-20603015-20021756","span":{"begin":66,"end":70},"obj":"20603015"},{"id":"24981866-11240135-20021757","span":{"begin":137,"end":141},"obj":"11240135"}],"attributes":[{"subj":"24981866-20603015-20021756","pred":"source","obj":"2_test"},{"subj":"24981866-11240135-20021757","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#e493ec","default":true}]}]}}