PMC:4024607 / 9536-10720 JSONTXT

{"project":"NEUROSES","denotations":[{"id":"T191","span":{"begin":316,"end":319},"obj":"CHEBI_81567"},{"id":"T192","span":{"begin":597,"end":600},"obj":"CHEBI_81567"},{"id":"T193","span":{"begin":736,"end":739},"obj":"CHEBI_81567"},{"id":"T194","span":{"begin":160,"end":166},"obj":"PATO_0001985"},{"id":"T195","span":{"begin":393,"end":399},"obj":"PATO_0001985"},{"id":"T196","span":{"begin":562,"end":567},"obj":"PATO_0000003"},{"id":"T197","span":{"begin":701,"end":706},"obj":"PATO_0000003"},{"id":"T198","span":{"begin":974,"end":979},"obj":"PATO_0001038"},{"id":"T199","span":{"begin":974,"end":979},"obj":"PATO_0001470"},{"id":"T200","span":{"begin":1001,"end":1011},"obj":"PATO_0002107"},{"id":"T183","span":{"begin":32,"end":39},"obj":"CHEBI_9584"},{"id":"T184","span":{"begin":40,"end":48},"obj":"PATO_0000173"},{"id":"T185","span":{"begin":83,"end":87},"obj":"PATO_0002316"},{"id":"T186","span":{"begin":109,"end":112},"obj":"CHEBI_17842"},{"id":"T187","span":{"begin":316,"end":319},"obj":"CHEBI_17842"},{"id":"T188","span":{"begin":597,"end":600},"obj":"CHEBI_17842"},{"id":"T189","span":{"begin":736,"end":739},"obj":"CHEBI_17842"},{"id":"T190","span":{"begin":109,"end":112},"obj":"CHEBI_81567"}],"text":"Neuroendocrine testing\nBaseline thyroid function was evaluated by measuring plasma free and bound T3, T4 and TSH levels. Venous blood was drawn and immediately frozen in aliquots at −70°C or below until analyzed. The samples were thawed and analyzed by immunoassays (Unicel DxI 800 Beckman Coulter, for FT4, FT3 and TSH and AutoDelfia, for T4 and T3) in the year 2010. No prior thawing of the frozen plasma samples had been performed. The Karolinska Laboratory at Karolinska University Hospital performed all analyses according to accredited routines. The intra-assay coefficient of variation for TSH was 3.85–5.56%, for FT4 2.74–4.4%, for FT3 5.1–6.6%, for T4 2.7–3.6% and for T3 2.9–3.1%. The inter-assay coefficient of variation for TSH was 3.02–3.68%, for FT4 3.34–8.08%, for FT3 1.3–8%, for T4 1.4–2.2% and for T3 1.2–2.1%. Analytical interferences in thyroid hormone testing are estimated to occur in less than 0.1%, at the Karolinska Clinical Laboratory. The FT3/FT4 ratio was used to estimate peripheral deiodination. Preanalytical variation was minimized by performing the venipuncture in a standardized manner for all participants, of which the majority was sampled at noon."}