PMC:400732 / 24757-26454 JSONTXT

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Digging was considered the actual physical displacement of sand with the forepaws and/or snout. Placing forelimbs on either of the dishes and sniffing was not considered digging and was not penalized or scored. Each completed trial was separated by a 20-sec inter-trial-interval (ITI). Preliminary experiments demonstrated that an extended time-out period following an incorrect response was not required to ensure goal-directed behavior in the wild-type or mutant mice for this particular task (data not shown). Therefore, the 20-sec ITI was used to separate all trials (successful and unsuccessful). The S+ and S- scents were counterbalanced across subjects in order to control for potential scent bias (pilot data suggest there are no biases, data not shown). 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