PMC:3991839 / 9213-10513 JSONTXT

{"project":"2_test","denotations":[{"id":"24765061-11584085-30743410","span":{"begin":1252,"end":1254},"obj":"11584085"}],"text":"Metabolites of various bacteria fermenting quercetin by HPLC and MS analysis\nIt is difficult to separate all the metabolites of bacteria by HPLC because of numerous substances in fermented broths. In this experiment, the metabolites of seven strains transforming quercetin were separated with HPLC. Only one compound was discovered, and it presented among the metabolites of both C. perfringens and B. fragilis at the retention time of 25.024 min in the charts (Fig. 1A and B). The retention time of quercetin was about 49.013 min. Its peak appeared in the fermented solution of E. gilvus, E. coli, S. lutetiensis, W. confuse, and L. acidophilus, but not of C. perfringens and B. fragilis.\nFig. 1 (a) Catabolites of C. perfringens fermenting quercetin, with HPLC analysis. S1 and S2 individually refer to the control and sample. (b) Catabolites of B. fragilis fermenting quercetin, with HPLC analysis. S1 and S2 individually refer to the control and sample. The component at the retention time of 25.024 min was collected for MS. Two significant molecular ion peaks of 167 and 123 m/z were observed (Fig. 2) and deduced as 3,4-dihydroxyphenylacetic acid, one main metabolite of gut bacterial fermenting quercetin, according to its possible fractures (16).\nFig. 2 Mass spectrum of the main compound."}

{"project":"MyTest","denotations":[{"id":"24765061-11584085-30743410","span":{"begin":1252,"end":1254},"obj":"11584085"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"Metabolites of various bacteria fermenting quercetin by HPLC and MS analysis\nIt is difficult to separate all the metabolites of bacteria by HPLC because of numerous substances in fermented broths. In this experiment, the metabolites of seven strains transforming quercetin were separated with HPLC. Only one compound was discovered, and it presented among the metabolites of both C. perfringens and B. fragilis at the retention time of 25.024 min in the charts (Fig. 1A and B). The retention time of quercetin was about 49.013 min. Its peak appeared in the fermented solution of E. gilvus, E. coli, S. lutetiensis, W. confuse, and L. acidophilus, but not of C. perfringens and B. fragilis.\nFig. 1 (a) Catabolites of C. perfringens fermenting quercetin, with HPLC analysis. S1 and S2 individually refer to the control and sample. (b) Catabolites of B. fragilis fermenting quercetin, with HPLC analysis. S1 and S2 individually refer to the control and sample. The component at the retention time of 25.024 min was collected for MS. Two significant molecular ion peaks of 167 and 123 m/z were observed (Fig. 2) and deduced as 3,4-dihydroxyphenylacetic acid, one main metabolite of gut bacterial fermenting quercetin, according to its possible fractures (16).\nFig. 2 Mass spectrum of the main compound."}