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analyses for sterols (measured enzymatically and thus reflecting total sterols) and triglycerides were performed on plasma samples from fasted animals fed a regular chow diet. No significant differences were observed for the sterol profiles (Figure 3a), but surprisingly, the Abcg8-/- knockout mice had a significantly higher triglyceride level in the LDL lipoprotein fractions compared to wild-type and heterozygous mice (Figure 3b). The size of this peak was variable between different littermate analyses, but is always increased in the knockout animals. Total plasma triglyceride levels of the Abcg8-/- mice were slightly higher (79.2 ± 14 mg/dL compared to the wild type 63.4 ± 13 mg/dL and heterozygotes 46.6 ± 12 mg/dL, n = 3 for all genotypes). Preliminary analyses of proteins in the isolated FPLC fractions did not show any qualitative changes in apolipoproteins B, E, AI, or AII. The significance of this triglyceride rich peak remains unclear at present."}

    craft-ca-core-ex-dev

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    craft-ca-core-dev

    {"project":"craft-ca-core-dev","denotations":[{"id":"T7965","span":{"begin":18,"end":25},"obj":"CHEBI:15889"},{"id":"T7966","span":{"begin":76,"end":83},"obj":"CHEBI:15889"},{"id":"T7967","span":{"begin":89,"end":102},"obj":"CHEBI:17855"},{"id":"T7968","span":{"begin":121,"end":127},"obj":"UBERON:0001969"},{"id":"T7969","span":{"begin":148,"end":155},"obj":"NCBITaxon:33208"},{"id":"T7970","span":{"begin":156,"end":159},"obj":"GO:0007631"},{"id":"T7971","span":{"begin":170,"end":174},"obj":"CHEBI:33290"},{"id":"T7972","span":{"begin":230,"end":236},"obj":"CHEBI:15889"},{"id":"T7973","span":{"begin":281,"end":286},"obj":"PR:000003573"},{"id":"T7974","span":{"begin":299,"end":303},"obj":"NCBITaxon:10088"},{"id":"T7975","span":{"begin":331,"end":343},"obj":"CHEBI:17855"},{"id":"T7976","span":{"begin":422,"end":426},"obj":"NCBITaxon:10088"},{"id":"T7977","span":{"begin":554,"end":561},"obj":"NCBITaxon:33208"},{"id":"T7978","span":{"begin":569,"end":575},"obj":"UBERON:0001969"},{"id":"T7979","span":{"begin":576,"end":588},"obj":"CHEBI:17855"},{"id":"T7980","span":{"begin":603,"end":608},"obj":"PR:000003573"},{"id":"T7981","span":{"begin":612,"end":616},"obj":"NCBITaxon:10088"},{"id":"T7982","span":{"begin":862,"end":877},"obj":"CHEBI:39015"},{"id":"T7983","span":{"begin":862,"end":879},"obj":"PR:000004145"},{"id":"T7984","span":{"begin":862,"end":877},"obj":"_FRAGMENT"},{"id":"T7985","span":{"begin":881,"end":882},"obj":"PR:000004155"},{"id":"T7986","span":{"begin":884,"end":886},"obj":"PR:000004140"},{"id":"T7987","span":{"begin":891,"end":894},"obj":"PR:000004142"},{"id":"T7988","span":{"begin":921,"end":933},"obj":"CHEBI:17855"}],"relations":[{"id":"R5277","pred":"_lexicallyChainedTo","subj":"T7985","obj":"T7984"},{"id":"R5278","pred":"_lexicallyChainedTo","subj":"T7986","obj":"T7984"},{"id":"R5279","pred":"_lexicallyChainedTo","subj":"T7987","obj":"T7984"}],"text":"FPLC analyses for sterols (measured enzymatically and thus reflecting total sterols) and triglycerides were performed on plasma samples from fasted animals fed a regular chow diet. No significant differences were observed for the sterol profiles (Figure 3a), but surprisingly, the Abcg8-/- knockout mice had a significantly higher triglyceride level in the LDL lipoprotein fractions compared to wild-type and heterozygous mice (Figure 3b). The size of this peak was variable between different littermate analyses, but is always increased in the knockout animals. Total plasma triglyceride levels of the Abcg8-/- mice were slightly higher (79.2 ± 14 mg/dL compared to the wild type 63.4 ± 13 mg/dL and heterozygotes 46.6 ± 12 mg/dL, n = 3 for all genotypes). Preliminary analyses of proteins in the isolated FPLC fractions did not show any qualitative changes in apolipoproteins B, E, AI, or AII. The significance of this triglyceride rich peak remains unclear at present."}