PMC:394351 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T258	8-13	NN	denotes	model
T259	0-110	sentence	denotes	A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol
T260	2-7	NN	denotes	mouse
T261	61-68	VBZ	denotes	results
T262	14-16	IN	denotes	of
T263	17-31	NN	denotes	sitosterolemia
T264	31-33	:	denotes	:
T265	33-40	NN	denotes	absence
T266	41-43	IN	denotes	of
T267	44-49	NN	denotes	Abcg8
T268	50-58	NN	denotes	sterolin
T269	49-50	HYPH	denotes	/
T270	58-59	HYPH	denotes	-
T271	59-60	CD	denotes	2
T272	69-71	IN	denotes	in
T273	72-79	NN	denotes	failure
T274	80-82	TO	denotes	to
T275	83-90	VB	denotes	secrete
T276	91-98	JJ	denotes	biliary
T277	99-110	NN	denotes	cholesterol
T278	110-111	sentence	denotes
T279	121-131	sentence	denotes	Background
T280	121-131	NN	denotes	Background
T281	131-431	sentence	denotes	Mutations in either of two genes comprising the STSL locus, ATP-binding cassette (ABC)-transporters ABCG5 (encoding sterolin-1) and ABCG8 (encoding sterolin-2), result in sitosterolemia, a rare autosomal recessive disorder of sterol trafficking characterized by increased plasma plant sterol levels.
T282	132-141	NNS	denotes	Mutations
T283	293-299	VBP	denotes	result
T284	142-144	IN	denotes	in
T285	145-151	DT	denotes	either
T286	152-154	IN	denotes	of
T287	155-158	CD	denotes	two
T288	159-164	NNS	denotes	genes
T289	165-175	VBG	denotes	comprising
T290	176-179	DT	denotes	the
T291	185-190	NN	denotes	locus
T292	180-184	NN	denotes	STSL
T293	190-192	,	denotes	,
T294	192-195	NN	denotes	ATP
T295	196-203	VBG	denotes	binding
T296	195-196	HYPH	denotes	-
T297	204-212	NN	denotes	cassette
T298	219-231	NNS	denotes	transporters
T299	213-214	-LRB-	denotes	(
T300	214-217	NN	denotes	ABC
T301	217-218	-RRB-	denotes	)
T302	218-219	HYPH	denotes	-
T303	232-237	NN	denotes	ABCG5
T304	238-239	-LRB-	denotes	(
T305	239-247	VBG	denotes	encoding
T306	248-256	NN	denotes	sterolin
T307	256-257	HYPH	denotes	-
T308	257-258	CD	denotes	1
T309	258-259	-RRB-	denotes	)
T310	260-263	CC	denotes	and
T311	264-269	NN	denotes	ABCG8
T312	270-271	-LRB-	denotes	(
T313	271-279	VBG	denotes	encoding
T314	280-288	NN	denotes	sterolin
T315	288-289	HYPH	denotes	-
T316	289-290	CD	denotes	2
T317	290-291	-RRB-	denotes	)
T318	291-293	,	denotes	,
T319	300-302	IN	denotes	in
T320	303-317	NN	denotes	sitosterolemia
T321	317-319	,	denotes	,
T322	319-320	DT	denotes	a
T323	346-354	NN	denotes	disorder
T324	321-325	JJ	denotes	rare
T325	326-335	JJ	denotes	autosomal
T326	336-345	JJ	denotes	recessive
T327	355-357	IN	denotes	of
T328	358-364	NN	denotes	sterol
T329	365-376	NN	denotes	trafficking
T330	377-390	VBN	denotes	characterized
T331	391-393	IN	denotes	by
T332	394-403	VBN	denotes	increased
T333	424-430	NNS	denotes	levels
T334	404-410	NN	denotes	plasma
T335	411-416	NN	denotes	plant
T336	417-423	NN	denotes	sterol
T337	430-431	.	denotes	.
T338	431-567	sentence	denotes	Based upon the genetics of sitosterolemia, ABCG5/sterolin-1 and ABCG8/sterolin-2 are hypothesized to function as obligate heterodimers.
T339	432-437	VBN	denotes	Based
T340	517-529	VBN	denotes	hypothesized
T341	438-442	IN	denotes	upon
T342	443-446	DT	denotes	the
T343	447-455	NN	denotes	genetics
T344	456-458	IN	denotes	of
T345	459-473	NN	denotes	sitosterolemia
T346	473-475	,	denotes	,
T347	475-480	NN	denotes	ABCG5
T348	481-489	NN	denotes	sterolin
T349	480-481	HYPH	denotes	/
T350	489-490	HYPH	denotes	-
T351	490-491	CD	denotes	1
T352	492-495	CC	denotes	and
T353	496-501	NN	denotes	ABCG8
T354	502-510	NN	denotes	sterolin
T355	501-502	HYPH	denotes	/
T356	510-511	HYPH	denotes	-
T357	511-512	CD	denotes	2
T358	513-516	VBP	denotes	are
T359	530-532	TO	denotes	to
T360	533-541	VB	denotes	function
T361	542-544	IN	denotes	as
T362	545-553	JJ	denotes	obligate
T363	554-566	NNS	denotes	heterodimers
T364	566-567	.	denotes	.
T365	567-673	sentence	denotes	No phenotypic difference has yet been described in humans with complete defects in either ABCG5 or ABCG8.
T366	568-570	DT	denotes	No
T367	582-592	NN	denotes	difference
T368	571-581	JJ	denotes	phenotypic
T369	606-615	VBN	denotes	described
T370	593-596	VBZ	denotes	has
T371	597-600	RB	denotes	yet
T372	601-605	VBN	denotes	been
T373	616-618	IN	denotes	in
T374	619-625	NNS	denotes	humans
T375	626-630	IN	denotes	with
T376	631-639	JJ	denotes	complete
T377	640-647	NNS	denotes	defects
T378	648-650	IN	denotes	in
T379	651-657	CC	denotes	either
T380	658-663	NN	denotes	ABCG5
T381	664-666	CC	denotes	or
T382	667-672	NN	denotes	ABCG8
T383	672-673	.	denotes	.
T384	673-805	sentence	denotes	These proteins, based upon the defects in humans, are responsible for regulating dietary sterol entry and biliary sterol secretion.
T385	674-679	DT	denotes	These
T386	680-688	NN	denotes	proteins
T387	724-727	VBP	denotes	are
T388	688-690	,	denotes	,
T389	690-695	VBN	denotes	based
T390	696-700	IN	denotes	upon
T391	701-704	DT	denotes	the
T392	705-712	NNS	denotes	defects
T393	713-715	IN	denotes	in
T394	716-722	NNS	denotes	humans
T395	722-724	,	denotes	,
T396	728-739	JJ	denotes	responsible
T397	740-743	IN	denotes	for
T398	744-754	VBG	denotes	regulating
T399	755-762	JJ	denotes	dietary
T400	770-775	NN	denotes	entry
T401	763-769	NN	denotes	sterol
T402	776-779	CC	denotes	and
T403	780-787	JJ	denotes	biliary
T404	795-804	NN	denotes	secretion
T405	788-794	NN	denotes	sterol
T406	804-805	.	denotes	.
T407	805-814	sentence	denotes	Methods
T408	807-814	NNS	denotes	Methods
T409	814-969	sentence	denotes	In order to mimic the human disease, we created, by a targeted disruption, a mouse model of sitosterolemia resulting in Abcg8/sterolin-2 deficiency alone.
T410	815-817	IN	denotes	In
T411	855-862	VBD	denotes	created
T412	818-823	NN	denotes	order
T413	824-826	TO	denotes	to
T414	827-832	VB	denotes	mimic
T415	833-836	DT	denotes	the
T416	843-850	NN	denotes	disease
T417	837-842	JJ	denotes	human
T418	850-852	,	denotes	,
T419	852-854	PRP	denotes	we
T420	862-864	,	denotes	,
T421	864-866	IN	denotes	by
T422	867-868	DT	denotes	a
T423	878-888	NN	denotes	disruption
T424	869-877	VBN	denotes	targeted
T425	888-890	,	denotes	,
T426	890-891	DT	denotes	a
T427	898-903	NN	denotes	model
T428	892-897	NN	denotes	mouse
T429	904-906	IN	denotes	of
T430	907-921	NN	denotes	sitosterolemia
T431	922-931	VBG	denotes	resulting
T432	932-934	IN	denotes	in
T433	935-940	NN	denotes	Abcg8
T434	941-949	NN	denotes	sterolin
T435	940-941	HYPH	denotes	/
T436	952-962	NN	denotes	deficiency
T437	949-950	HYPH	denotes	-
T438	950-951	CD	denotes	2
T439	963-968	RB	denotes	alone
T440	968-969	.	denotes	.
T441	969-1033	sentence	denotes	Homozygous knockout mice are viable and exhibit sitosterolemia.
T442	970-980	JJ	denotes	Homozygous
T443	990-994	NNS	denotes	mice
T444	981-989	NN	denotes	knockout
T445	995-998	VBP	denotes	are
T446	999-1005	JJ	denotes	viable
T447	1006-1009	CC	denotes	and
T448	1010-1017	VBP	denotes	exhibit
T449	1018-1032	NN	denotes	sitosterolemia
T450	1032-1033	.	denotes	.
T451	1033-1042	sentence	denotes	Results
T452	1035-1042	NNS	denotes	Results
T453	1042-1194	sentence	denotes	Mice deficient in Abcg8 have significantly increased plasma and tissue plant sterol levels (sitosterol and campesterol) consistent with sitosterolemia.
T454	1043-1047	NNS	denotes	Mice
T455	1067-1071	VBP	denotes	have
T456	1048-1057	JJ	denotes	deficient
T457	1058-1060	IN	denotes	in
T458	1061-1066	NN	denotes	Abcg8
T459	1072-1085	RB	denotes	significantly
T460	1086-1095	VBN	denotes	increased
T461	1096-1102	NN	denotes	plasma
T462	1103-1106	CC	denotes	and
T463	1107-1113	NN	denotes	tissue
T464	1120-1126	NN	denotes	sterol
T465	1114-1119	NN	denotes	plant
T466	1127-1133	NNS	denotes	levels
T467	1134-1135	-LRB-	denotes	(
T468	1135-1145	NN	denotes	sitosterol
T469	1146-1149	CC	denotes	and
T470	1150-1161	NN	denotes	campesterol
T471	1161-1162	-RRB-	denotes	)
T472	1163-1173	JJ	denotes	consistent
T473	1174-1178	IN	denotes	with
T474	1179-1193	NN	denotes	sitosterolemia
T475	1193-1194	.	denotes	.
T476	1194-1347	sentence	denotes	Interestingly, Abcg5/sterolin-1 was expressed in both liver and intestine in Abcg8/sterolin-2 deficient mice and continued to show an apical expression.
T477	1195-1208	RB	denotes	Interestingly
T478	1231-1240	VBN	denotes	expressed
T479	1208-1210	,	denotes	,
T480	1210-1215	NN	denotes	Abcg5
T481	1216-1224	NN	denotes	sterolin
T482	1215-1216	HYPH	denotes	/
T483	1224-1225	HYPH	denotes	-
T484	1225-1226	CD	denotes	1
T485	1227-1230	VBD	denotes	was
T486	1241-1243	IN	denotes	in
T487	1244-1248	CC	denotes	both
T488	1249-1254	NN	denotes	liver
T489	1255-1258	CC	denotes	and
T490	1259-1268	NN	denotes	intestine
T491	1269-1271	IN	denotes	in
T492	1272-1277	NN	denotes	Abcg8
T493	1278-1286	NN	denotes	sterolin
T494	1277-1278	HYPH	denotes	/
T495	1289-1298	JJ	denotes	deficient
T496	1286-1287	HYPH	denotes	-
T497	1287-1288	CD	denotes	2
T498	1299-1303	NNS	denotes	mice
T499	1304-1307	CC	denotes	and
T500	1308-1317	VBD	denotes	continued
T501	1318-1320	TO	denotes	to
T502	1321-1325	VB	denotes	show
T503	1326-1328	DT	denotes	an
T504	1336-1346	NN	denotes	expression
T505	1329-1335	JJ	denotes	apical
T506	1346-1347	.	denotes	.
T507	1347-1494	sentence	denotes	Remarkably, Abcg8 deficient mice had an impaired ability to secrete cholesterol into bile, but still maintained the ability to secrete sitosterol.
T508	1348-1358	RB	denotes	Remarkably
T509	1381-1384	VBD	denotes	had
T510	1358-1360	,	denotes	,
T511	1360-1365	NN	denotes	Abcg8
T512	1366-1375	JJ	denotes	deficient
T513	1376-1380	NNS	denotes	mice
T514	1385-1387	DT	denotes	an
T515	1397-1404	NN	denotes	ability
T516	1388-1396	JJ	denotes	impaired
T517	1405-1407	TO	denotes	to
T518	1408-1415	VB	denotes	secrete
T519	1416-1427	NN	denotes	cholesterol
T520	1428-1432	IN	denotes	into
T521	1433-1437	NN	denotes	bile
T522	1437-1439	,	denotes	,
T523	1439-1442	CC	denotes	but
T524	1443-1448	RB	denotes	still
T525	1449-1459	VBD	denotes	maintained
T526	1460-1463	DT	denotes	the
T527	1464-1471	NN	denotes	ability
T528	1472-1474	TO	denotes	to
T529	1475-1482	VB	denotes	secrete
T530	1483-1493	NN	denotes	sitosterol
T531	1493-1494	.	denotes	.
T532	1494-1681	sentence	denotes	We also report an intermediate phenotype in the heterozygous Abcg8+/- mice that are not sitosterolemic, but have a decreased level of biliary sterol secretion relative to wild-type mice.
T533	1495-1497	PRP	denotes	We
T534	1503-1509	VBP	denotes	report
T535	1498-1502	RB	denotes	also
T536	1510-1512	DT	denotes	an
T537	1526-1535	NN	denotes	phenotype
T538	1513-1525	JJ	denotes	intermediate
T539	1536-1538	IN	denotes	in
T540	1539-1542	DT	denotes	the
T541	1565-1569	NNS	denotes	mice
T542	1543-1555	JJ	denotes	heterozygous
T543	1556-1561	NN	denotes	Abcg8
T544	1561-1562	SYM	denotes	+
T545	1562-1563	HYPH	denotes	/
T546	1563-1564	SYM	denotes	-
T547	1570-1574	WDT	denotes	that
T548	1575-1578	VBP	denotes	are
T549	1579-1582	RB	denotes	not
T550	1583-1597	JJ	denotes	sitosterolemic
T551	1597-1599	,	denotes	,
T552	1599-1602	CC	denotes	but
T553	1603-1607	VBP	denotes	have
T554	1608-1609	DT	denotes	a
T555	1620-1625	NN	denotes	level
T556	1610-1619	VBN	denotes	decreased
T557	1626-1628	IN	denotes	of
T558	1629-1636	JJ	denotes	biliary
T559	1644-1653	NN	denotes	secretion
T560	1637-1643	NN	denotes	sterol
T561	1654-1662	JJ	denotes	relative
T562	1663-1665	IN	denotes	to
T563	1666-1670	JJ	denotes	wild
T564	1671-1675	NN	denotes	type
T565	1670-1671	HYPH	denotes	-
T566	1676-1680	NNS	denotes	mice
T567	1680-1681	.	denotes	.
T568	1681-1693	sentence	denotes	Conclusion
T569	1683-1693	NN	denotes	Conclusion
T570	1693-1890	sentence	denotes	These data indicate that Abcg8/sterolin-2 is necessary for biliary sterol secretion and that loss of Abcg8/sterolin-2 has a more profound effect upon biliary cholesterol secretion than sitosterol.
T571	1694-1699	DT	denotes	These
T572	1700-1704	NNS	denotes	data
T573	1705-1713	VBP	denotes	indicate
T574	1714-1718	IN	denotes	that
T575	1736-1738	VBZ	denotes	is
T576	1719-1724	NN	denotes	Abcg8
T577	1725-1733	NN	denotes	sterolin
T578	1724-1725	HYPH	denotes	/
T579	1733-1734	HYPH	denotes	-
T580	1734-1735	CD	denotes	2
T581	1739-1748	JJ	denotes	necessary
T582	1749-1752	IN	denotes	for
T583	1753-1760	JJ	denotes	biliary
T584	1768-1777	NN	denotes	secretion
T585	1761-1767	NN	denotes	sterol
T586	1778-1781	CC	denotes	and
T587	1782-1786	IN	denotes	that
T588	1812-1815	VBZ	denotes	has
T589	1787-1791	NN	denotes	loss
T590	1792-1794	IN	denotes	of
T591	1795-1800	NN	denotes	Abcg8
T592	1801-1809	NN	denotes	sterolin
T593	1800-1801	HYPH	denotes	/
T594	1809-1810	HYPH	denotes	-
T595	1810-1811	CD	denotes	2
T596	1816-1817	DT	denotes	a
T597	1832-1838	NN	denotes	effect
T598	1818-1822	RBR	denotes	more
T599	1823-1831	JJ	denotes	profound
T600	1839-1843	IN	denotes	upon
T601	1844-1851	JJ	denotes	biliary
T602	1864-1873	NN	denotes	secretion
T603	1852-1863	NN	denotes	cholesterol
T604	1874-1878	IN	denotes	than
T605	1879-1889	NN	denotes	sitosterol
T606	1889-1890	.	denotes	.
T607	1890-2110	sentence	denotes	Since biliary sitosterol secretion is preserved, although not elevated in the sitosterolemic mice, this observation suggests that mechanisms other than by Abcg8/sterolin-2 may be responsible for its secretion into bile.
T608	1891-1896	IN	denotes	Since
T609	1929-1938	VBN	denotes	preserved
T610	1897-1904	JJ	denotes	biliary
T611	1916-1925	NN	denotes	secretion
T612	1905-1915	NN	denotes	sitosterol
T613	1926-1928	VBZ	denotes	is
T614	2007-2015	VBZ	denotes	suggests
T615	1938-1940	,	denotes	,
T616	1940-1948	IN	denotes	although
T617	1953-1961	JJ	denotes	elevated
T618	1949-1952	RB	denotes	not
T619	1962-1964	IN	denotes	in
T620	1965-1968	DT	denotes	the
T621	1984-1988	NNS	denotes	mice
T622	1969-1983	JJ	denotes	sitosterolemic
T623	1988-1990	,	denotes	,
T624	1990-1994	DT	denotes	this
T625	1995-2006	NN	denotes	observation
T626	2016-2020	IN	denotes	that
T627	2067-2069	VB	denotes	be
T628	2021-2031	NNS	denotes	mechanisms
T629	2032-2037	JJ	denotes	other
T630	2038-2042	IN	denotes	than
T631	2043-2045	IN	denotes	by
T632	2046-2051	NN	denotes	Abcg8
T633	2052-2060	NN	denotes	sterolin
T634	2051-2052	HYPH	denotes	/
T635	2060-2061	HYPH	denotes	-
T636	2061-2062	CD	denotes	2
T637	2063-2066	MD	denotes	may
T638	2070-2081	JJ	denotes	responsible
T639	2082-2085	IN	denotes	for
T640	2086-2089	PRP$	denotes	its
T641	2090-2099	NN	denotes	secretion
T642	2100-2104	IN	denotes	into
T643	2105-2109	NN	denotes	bile
T644	2109-2110	.	denotes	.
T5050	18233-18235	IN	denotes	in
T1078	2123-2133	NN	denotes	Absorption
T1079	2176-2178	VBZ	denotes	is
T1080	2134-2136	IN	denotes	of
T1081	2137-2144	JJ	denotes	dietary
T1082	2145-2156	NN	denotes	cholesterol
T1083	2157-2161	IN	denotes	from
T1084	2162-2165	DT	denotes	the
T1085	2166-2175	NN	denotes	intestine
T1086	2179-2181	DT	denotes	an
T1087	2192-2196	NN	denotes	part
T1088	2182-2191	JJ	denotes	important
T1089	2197-2199	IN	denotes	of
T1090	2200-2211	NN	denotes	cholesterol
T1091	2212-2223	NN	denotes	homeostasis
T1092	2224-2227	CC	denotes	and
T1093	2228-2238	VBZ	denotes	represents
T1094	2239-2242	DT	denotes	the
T1095	2251-2255	NN	denotes	step
T1096	2243-2250	JJ	denotes	initial
T1097	2256-2260	WDT	denotes	that
T1098	2261-2267	VBZ	denotes	allows
T1099	2268-2275	JJ	denotes	dietary
T1100	2276-2287	NN	denotes	cholesterol
T1101	2291-2296	VB	denotes	exert
T1102	2288-2290	TO	denotes	to
T1103	2297-2300	PRP$	denotes	its
T1104	2311-2318	NNS	denotes	effects
T1105	2301-2310	JJ	denotes	metabolic
T1106	2318-2319	.	denotes	.
T1107	2319-2575	sentence	denotes	A typical western diet contains relatively equal amounts of cholesterol and non-cholesterol sterols, mainly plant sterols, of which about 55% of the dietary cholesterol is absorbed and retained compared to ~1% of the dietary non-cholesterol sterols [1-3].
T1108	2320-2321	DT	denotes	A
T1109	2338-2342	NN	denotes	diet
T1110	2322-2329	JJ	denotes	typical
T1111	2330-2337	NNP	denotes	western
T1112	2343-2351	VBZ	denotes	contains
T1113	2352-2362	RB	denotes	relatively
T1114	2363-2368	JJ	denotes	equal
T1115	2369-2376	NNS	denotes	amounts
T1116	2377-2379	IN	denotes	of
T1117	2380-2391	NN	denotes	cholesterol
T1118	2412-2419	NNS	denotes	sterols
T1119	2392-2395	CC	denotes	and
T1120	2396-2411	JJ	denotes	non-cholesterol
T1121	2419-2421	,	denotes	,
T1122	2421-2427	RB	denotes	mainly
T1123	2434-2441	NNS	denotes	sterols
T1124	2428-2433	NN	denotes	plant
T1125	2441-2443	,	denotes	,
T1126	2443-2445	IN	denotes	of
T1127	2492-2500	VBN	denotes	absorbed
T1128	2446-2451	WDT	denotes	which
T1129	2452-2457	IN	denotes	about
T1130	2458-2460	CD	denotes	55
T1131	2460-2461	NN	denotes	%
T1132	2462-2464	IN	denotes	of
T1133	2465-2468	DT	denotes	the
T1134	2477-2488	NN	denotes	cholesterol
T1135	2469-2476	JJ	denotes	dietary
T1136	2489-2491	VBZ	denotes	is
T1137	2501-2504	CC	denotes	and
T1138	2505-2513	VBN	denotes	retained
T1139	2514-2522	VBN	denotes	compared
T1140	2523-2525	IN	denotes	to
T1141	2526-2527	SYM	denotes	~
T1142	2527-2528	CD	denotes	1
T1143	2528-2529	NN	denotes	%
T1144	2530-2532	IN	denotes	of
T1145	2533-2536	DT	denotes	the
T1146	2561-2568	NNS	denotes	sterols
T1147	2537-2544	JJ	denotes	dietary
T1148	2545-2560	JJ	denotes	non-cholesterol
T1149	2569-2570	-LRB-	denotes	[
T1150	2570-2571	CD	denotes	1
T1151	2571-2572	SYM	denotes	-
T1152	2572-2573	CD	denotes	3
T1153	2573-2574	-RRB-	denotes	]
T1154	2574-2575	.	denotes	.
T1155	2575-2831	sentence	denotes	Schoenheimer recognized more than 75 years ago that only cholesterol, not non-cholesterol sterols, is absorbed in the intestine, although the exact molecular mechanisms by which preferential cholesterol absorption occurs has not been fully elucidated [4].
T1156	2576-2588	NNP	denotes	Schoenheimer
T1157	2589-2599	VBD	denotes	recognized
T1158	2600-2604	JJR	denotes	more
T1159	2610-2612	CD	denotes	75
T1160	2605-2609	IN	denotes	than
T1161	2613-2618	NNS	denotes	years
T1162	2619-2622	RB	denotes	ago
T1163	2623-2627	IN	denotes	that
T1164	2678-2686	VBN	denotes	absorbed
T1165	2628-2632	RB	denotes	only
T1166	2633-2644	NN	denotes	cholesterol
T1167	2644-2646	,	denotes	,
T1168	2646-2649	CC	denotes	not
T1169	2650-2665	JJ	denotes	non-cholesterol
T1170	2666-2673	NNS	denotes	sterols
T1171	2673-2675	,	denotes	,
T1172	2675-2677	VBZ	denotes	is
T1173	2687-2689	IN	denotes	in
T1174	2690-2693	DT	denotes	the
T1175	2694-2703	NN	denotes	intestine
T1176	2703-2705	,	denotes	,
T1177	2705-2713	IN	denotes	although
T1178	2816-2826	VBN	denotes	elucidated
T1179	2714-2717	DT	denotes	the
T1180	2734-2744	NNS	denotes	mechanisms
T1181	2718-2723	JJ	denotes	exact
T1182	2724-2733	JJ	denotes	molecular
T1183	2745-2747	IN	denotes	by
T1184	2790-2796	VBZ	denotes	occurs
T1185	2748-2753	WDT	denotes	which
T1186	2754-2766	JJ	denotes	preferential
T1187	2779-2789	NN	denotes	absorption
T1188	2767-2778	NN	denotes	cholesterol
T1189	2797-2800	VBZ	denotes	has
T1190	2801-2804	RB	denotes	not
T1191	2805-2809	VBN	denotes	been
T1192	2810-2815	RB	denotes	fully
T1193	2827-2828	-LRB-	denotes	[
T1194	2828-2829	CD	denotes	4
T1195	2829-2830	-RRB-	denotes	]
T1196	2830-2831	.	denotes	.
T1197	2831-3033	sentence	denotes	Similarly, although the liver secretes free cholesterol into bile, it can preferentially excrete non-cholesterol sterols into bile and the mechanism(s) of this process has yet to be elucidated as well.
T1198	2832-2841	RB	denotes	Similarly
T1199	2921-2928	VB	denotes	excrete
T1200	2841-2843	,	denotes	,
T1201	2843-2851	IN	denotes	although
T1202	2862-2870	VBZ	denotes	secretes
T1203	2852-2855	DT	denotes	the
T1204	2856-2861	NN	denotes	liver
T1205	2871-2875	JJ	denotes	free
T1206	2876-2887	NN	denotes	cholesterol
T1207	2888-2892	IN	denotes	into
T1208	2893-2897	NN	denotes	bile
T1209	2897-2899	,	denotes	,
T1210	2899-2901	PRP	denotes	it
T1211	2902-2905	MD	denotes	can
T1212	2906-2920	RB	denotes	preferentially
T1213	2929-2944	JJ	denotes	non-cholesterol
T1214	2945-2952	NNS	denotes	sterols
T1215	2953-2957	IN	denotes	into
T1216	2958-2962	NN	denotes	bile
T1217	2963-2966	CC	denotes	and
T1218	2967-2970	DT	denotes	the
T1219	2971-2980	NN	denotes	mechanism
T1220	3000-3003	VBZ	denotes	has
T1221	2980-2981	-LRB-	denotes	(
T1222	2981-2982	AFX	denotes	s
T1223	2982-2983	-RRB-	denotes	)
T1224	2984-2986	IN	denotes	of
T1225	2987-2991	DT	denotes	this
T1226	2992-2999	NN	denotes	process
T1227	3004-3007	RB	denotes	yet
T1228	3008-3010	TO	denotes	to
T1229	3014-3024	VBN	denotes	elucidated
T1230	3011-3013	VB	denotes	be
T1231	3025-3027	RB	denotes	as
T1232	3028-3032	RB	denotes	well
T1233	3032-3033	.	denotes	.
T1234	3033-3133	sentence	denotes	Only recently, through the study of a rare human disease, have clues to this process been revealed.
T1235	3034-3038	RB	denotes	Only
T1236	3039-3047	RB	denotes	recently
T1237	3124-3132	VBN	denotes	revealed
T1238	3047-3049	,	denotes	,
T1239	3049-3056	IN	denotes	through
T1240	3057-3060	DT	denotes	the
T1241	3061-3066	NN	denotes	study
T1242	3067-3069	IN	denotes	of
T1243	3070-3071	DT	denotes	a
T1244	3083-3090	NN	denotes	disease
T1245	3072-3076	JJ	denotes	rare
T1246	3077-3082	JJ	denotes	human
T1247	3090-3092	,	denotes	,
T1248	3092-3096	VB	denotes	have
T1249	3097-3102	NNS	denotes	clues
T1250	3103-3105	IN	denotes	to
T1251	3106-3110	DT	denotes	this
T1252	3111-3118	NN	denotes	process
T1253	3119-3123	VBN	denotes	been
T1254	3132-3133	.	denotes	.
T1255	3133-3312	sentence	denotes	Sitosterolemia (MIM 210250, also known as phytosterolemia) is a rare autosomal, recessively inherited disorder characterized by elevated blood and tissue plant sterol levels [5].
T1256	3134-3148	NN	denotes	Sitosterolemia
T1257	3193-3195	VBZ	denotes	is
T1258	3149-3150	-LRB-	denotes	(
T1259	3150-3153	NN	denotes	MIM
T1260	3154-3160	CD	denotes	210250
T1261	3160-3162	,	denotes	,
T1262	3162-3166	RB	denotes	also
T1263	3167-3172	VBN	denotes	known
T1264	3173-3175	IN	denotes	as
T1265	3176-3191	NN	denotes	phytosterolemia
T1266	3191-3192	-RRB-	denotes	)
T1267	3196-3197	DT	denotes	a
T1268	3236-3244	NN	denotes	disorder
T1269	3198-3202	JJ	denotes	rare
T1270	3203-3212	JJ	denotes	autosomal
T1271	3212-3214	,	denotes	,
T1272	3214-3225	RB	denotes	recessively
T1273	3226-3235	VBN	denotes	inherited
T1274	3245-3258	VBN	denotes	characterized
T1275	3259-3261	IN	denotes	by
T1276	3262-3270	VBN	denotes	elevated
T1277	3301-3307	NNS	denotes	levels
T1278	3271-3276	NN	denotes	blood
T1279	3277-3280	CC	denotes	and
T1280	3281-3287	NN	denotes	tissue
T1281	3288-3293	NN	denotes	plant
T1282	3294-3300	NN	denotes	sterol
T1283	3308-3309	-LRB-	denotes	[
T1284	3309-3310	CD	denotes	5
T1285	3310-3311	-RRB-	denotes	]
T1286	3311-3312	.	denotes	.
T1287	3312-3460	sentence	denotes	Affected individuals can develop tendon and/or tuberous xanthomas, hemolytic episodes, arthralgias, arthritis, and premature atherosclerosis [6,7].
T1288	3313-3321	VBN	denotes	Affected
T1289	3322-3333	NNS	denotes	individuals
T1290	3338-3345	VB	denotes	develop
T1291	3334-3337	MD	denotes	can
T1292	3346-3352	NN	denotes	tendon
T1293	3369-3378	NNS	denotes	xanthomas
T1294	3353-3356	CC	denotes	and
T1295	3356-3357	HYPH	denotes	/
T1296	3357-3359	CC	denotes	or
T1297	3360-3368	JJ	denotes	tuberous
T1298	3378-3380	,	denotes	,
T1299	3380-3389	JJ	denotes	hemolytic
T1300	3390-3398	NNS	denotes	episodes
T1301	3398-3400	,	denotes	,
T1302	3400-3411	NNS	denotes	arthralgias
T1303	3411-3413	,	denotes	,
T1304	3413-3422	NN	denotes	arthritis
T1305	3422-3424	,	denotes	,
T1306	3424-3427	CC	denotes	and
T1307	3428-3437	JJ	denotes	premature
T1308	3438-3453	NN	denotes	atherosclerosis
T1309	3454-3455	-LRB-	denotes	[
T1310	3455-3456	CD	denotes	6
T1311	3457-3458	CD	denotes	7
T1312	3456-3457	,	denotes	,
T1313	3458-3459	-RRB-	denotes	]
T1314	3459-3460	.	denotes	.
T1315	3460-3744	sentence	denotes	Sitosterolemic patients have diagnostically elevated plasma plant sterol levels (for example, sitosterol, campesterol, stigmasterol, and avenosterol, etc.) and their 5-saturated stanol metabolites, particularly sitostanol, with normal or only moderately increased cholesterol levels.
T1316	3461-3475	JJ	denotes	Sitosterolemic
T1317	3476-3484	NNS	denotes	patients
T1318	3485-3489	VBP	denotes	have
T1319	3490-3504	RB	denotes	diagnostically
T1320	3505-3513	VBN	denotes	elevated
T1321	3534-3540	NNS	denotes	levels
T1322	3514-3520	NN	denotes	plasma
T1323	3527-3533	NN	denotes	sterol
T1324	3521-3526	NN	denotes	plant
T1325	3541-3542	-LRB-	denotes	(
T1326	3542-3545	IN	denotes	for
T1327	3555-3565	NN	denotes	sitosterol
T1328	3546-3553	NN	denotes	example
T1329	3553-3555	,	denotes	,
T1330	3565-3567	,	denotes	,
T1331	3567-3578	NN	denotes	campesterol
T1332	3578-3580	,	denotes	,
T1333	3580-3592	NN	denotes	stigmasterol
T1334	3592-3594	,	denotes	,
T1335	3594-3597	CC	denotes	and
T1336	3598-3609	NN	denotes	avenosterol
T1337	3609-3611	,	denotes	,
T1338	3611-3615	FW	denotes	etc.
T1339	3615-3616	-RRB-	denotes	)
T1340	3617-3620	CC	denotes	and
T1341	3621-3626	PRP$	denotes	their
T1342	3646-3657	NNS	denotes	metabolites
T1343	3627-3628	CD	denotes	5
T1344	3629-3638	VBN	denotes	saturated
T1345	3628-3629	HYPH	denotes	-
T1346	3639-3645	NN	denotes	stanol
T1347	3657-3659	,	denotes	,
T1348	3659-3671	RB	denotes	particularly
T1349	3672-3682	NN	denotes	sitostanol
T1350	3682-3684	,	denotes	,
T1351	3684-3688	IN	denotes	with
T1352	3689-3695	JJ	denotes	normal
T1353	3737-3743	NNS	denotes	levels
T1354	3696-3698	CC	denotes	or
T1355	3699-3703	RB	denotes	only
T1356	3704-3714	RB	denotes	moderately
T1357	3715-3724	VBN	denotes	increased
T1358	3725-3736	NN	denotes	cholesterol
T1359	3743-3744	.	denotes	.
T1360	3744-3961	sentence	denotes	Clinical studies have shown that affected individuals hyperabsorb all dietary sterols (thus have lost the ability to restrict the sterol species absorbed) and have lost the ability to excrete sterols into bile [8,9].
T1361	3745-3753	JJ	denotes	Clinical
T1362	3754-3761	NNS	denotes	studies
T1363	3767-3772	VBN	denotes	shown
T1364	3762-3766	VBP	denotes	have
T1365	3773-3777	IN	denotes	that
T1366	3799-3810	VBP	denotes	hyperabsorb
T1367	3778-3786	VBN	denotes	affected
T1368	3787-3798	NNS	denotes	individuals
T1369	3811-3814	DT	denotes	all
T1370	3823-3830	NNS	denotes	sterols
T1371	3815-3822	JJ	denotes	dietary
T1372	3831-3832	-LRB-	denotes	(
T1373	3842-3846	VBN	denotes	lost
T1374	3832-3836	RB	denotes	thus
T1375	3837-3841	VBP	denotes	have
T1376	3847-3850	DT	denotes	the
T1377	3851-3858	NN	denotes	ability
T1378	3859-3861	TO	denotes	to
T1379	3862-3870	VB	denotes	restrict
T1380	3871-3874	DT	denotes	the
T1381	3882-3889	NNS	denotes	species
T1382	3875-3881	NN	denotes	sterol
T1383	3890-3898	VBN	denotes	absorbed
T1384	3898-3899	-RRB-	denotes	)
T1385	3900-3903	CC	denotes	and
T1386	3904-3908	VBP	denotes	have
T1387	3909-3913	VBN	denotes	lost
T1388	3914-3917	DT	denotes	the
T1389	3918-3925	NN	denotes	ability
T1390	3926-3928	TO	denotes	to
T1391	3929-3936	VB	denotes	excrete
T1392	3937-3944	NNS	denotes	sterols
T1393	3945-3949	IN	denotes	into
T1394	3950-3954	NN	denotes	bile
T1395	3955-3956	-LRB-	denotes	[
T1396	3958-3959	CD	denotes	9
T1397	3956-3957	CD	denotes	8
T1398	3957-3958	,	denotes	,
T1399	3959-3960	-RRB-	denotes	]
T1400	3960-3961	.	denotes	.
T1401	3961-4176	sentence	denotes	Infusion of sitosterol and cholesterol into normal individuals leads to a rapid and preferential excretion of sitosterol into bile, but in sitosterolemic individuals biliary sterol secretion is almost absent [7,9].
T1402	3962-3970	NN	denotes	Infusion
T1403	4025-4030	VBZ	denotes	leads
T1404	3971-3973	IN	denotes	of
T1405	3974-3984	NN	denotes	sitosterol
T1406	3985-3988	CC	denotes	and
T1407	3989-4000	NN	denotes	cholesterol
T1408	4001-4005	IN	denotes	into
T1409	4006-4012	JJ	denotes	normal
T1410	4013-4024	NNS	denotes	individuals
T1411	4031-4033	IN	denotes	to
T1412	4034-4035	DT	denotes	a
T1413	4059-4068	NN	denotes	excretion
T1414	4036-4041	JJ	denotes	rapid
T1415	4042-4045	CC	denotes	and
T1416	4046-4058	JJ	denotes	preferential
T1417	4069-4071	IN	denotes	of
T1418	4072-4082	NN	denotes	sitosterol
T1419	4083-4087	IN	denotes	into
T1420	4088-4092	NN	denotes	bile
T1421	4092-4094	,	denotes	,
T1422	4094-4097	CC	denotes	but
T1423	4098-4100	IN	denotes	in
T1424	4153-4155	VBZ	denotes	is
T1425	4101-4115	JJ	denotes	sitosterolemic
T1426	4116-4127	NNS	denotes	individuals
T1427	4128-4135	JJ	denotes	biliary
T1428	4136-4142	NN	denotes	sterol
T1429	4143-4152	NN	denotes	secretion
T1430	4156-4162	RB	denotes	almost
T1431	4163-4169	JJ	denotes	absent
T1432	4170-4171	-LRB-	denotes	[
T1433	4173-4174	CD	denotes	9
T1434	4171-4172	CD	denotes	7
T1435	4172-4173	,	denotes	,
T1436	4174-4175	-RRB-	denotes	]
T1437	4175-4176	.	denotes	.
T1438	4176-4367	sentence	denotes	Previous studies on sitosterolemia have suggested a defect involving a putative sterol 'transporter' expressed in the intestinal brush border and/or the hepatic canalicular membrane [10,11].
T1439	4177-4185	JJ	denotes	Previous
T1440	4186-4193	NNS	denotes	studies
T1441	4217-4226	VBN	denotes	suggested
T1442	4194-4196	IN	denotes	on
T1443	4197-4211	NN	denotes	sitosterolemia
T1444	4212-4216	VBP	denotes	have
T1445	4227-4228	DT	denotes	a
T1446	4229-4235	NN	denotes	defect
T1447	4236-4245	VBG	denotes	involving
T1448	4246-4247	DT	denotes	a
T1449	4265-4276	NN	denotes	transporter
T1450	4248-4256	JJ	denotes	putative
T1451	4257-4263	NN	denotes	sterol
T1452	4264-4265	``	denotes	'
T1453	4276-4277	''	denotes	'
T1454	4278-4287	VBN	denotes	expressed
T1455	4288-4290	IN	denotes	in
T1456	4291-4294	DT	denotes	the
T1457	4312-4318	NN	denotes	border
T1458	4295-4305	JJ	denotes	intestinal
T1459	4306-4311	NN	denotes	brush
T1460	4319-4322	CC	denotes	and
T1461	4322-4323	HYPH	denotes	/
T1462	4323-4325	CC	denotes	or
T1463	4326-4329	DT	denotes	the
T1464	4350-4358	NN	denotes	membrane
T1465	4330-4337	JJ	denotes	hepatic
T1466	4338-4349	JJ	denotes	canalicular
T1467	4359-4360	-LRB-	denotes	[
T1468	4363-4365	CD	denotes	11
T1469	4360-4362	CD	denotes	10
T1470	4362-4363	,	denotes	,
T1471	4365-4366	-RRB-	denotes	]
T1472	4366-4367	.	denotes	.
T1473	4367-4513	sentence	denotes	Genetic analyses of sitosterolemia pedigrees allowed the mapping of the STSL locus to human chromosome 2p21, between D2S2294 and D2S2298 [12,13].
T1474	4368-4375	JJ	denotes	Genetic
T1475	4376-4384	NNS	denotes	analyses
T1476	4413-4420	VBD	denotes	allowed
T1477	4385-4387	IN	denotes	of
T1478	4388-4402	NN	denotes	sitosterolemia
T1479	4403-4412	NNS	denotes	pedigrees
T1480	4421-4424	DT	denotes	the
T1481	4425-4432	NN	denotes	mapping
T1482	4433-4435	IN	denotes	of
T1483	4436-4439	DT	denotes	the
T1484	4445-4450	NN	denotes	locus
T1485	4440-4444	NN	denotes	STSL
T1486	4451-4453	IN	denotes	to
T1487	4454-4459	JJ	denotes	human
T1488	4471-4475	NN	denotes	2p21
T1489	4460-4470	NN	denotes	chromosome
T1490	4475-4477	,	denotes	,
T1491	4477-4484	IN	denotes	between
T1492	4485-4492	NN	denotes	D2S2294
T1493	4493-4496	CC	denotes	and
T1494	4497-4504	NN	denotes	D2S2298
T1495	4505-4506	-LRB-	denotes	[
T1496	4509-4511	CD	denotes	13
T1497	4506-4508	CD	denotes	12
T1498	4508-4509	,	denotes	,
T1499	4511-4512	-RRB-	denotes	]
T1500	4512-4513	.	denotes	.
T1501	4513-4779	sentence	denotes	By using positional cloning procedures or by screening for genes induced by exposure to LXR agonists, two groups identified not one but two genes, ABCG5 and ABCG8, which encode the proteins sterolin-1 and sterolin-2, mutations of which cause sitosterolemia [14-16].
T1502	4514-4516	IN	denotes	By
T1503	4627-4637	VBD	denotes	identified
T1504	4517-4522	VBG	denotes	using
T1505	4523-4533	JJ	denotes	positional
T1506	4534-4541	NN	denotes	cloning
T1507	4542-4552	NNS	denotes	procedures
T1508	4553-4555	CC	denotes	or
T1509	4556-4558	IN	denotes	by
T1510	4559-4568	VBG	denotes	screening
T1511	4569-4572	IN	denotes	for
T1512	4573-4578	NNS	denotes	genes
T1513	4579-4586	VBN	denotes	induced
T1514	4587-4589	IN	denotes	by
T1515	4590-4598	NN	denotes	exposure
T1516	4599-4601	IN	denotes	to
T1517	4602-4605	NN	denotes	LXR
T1518	4606-4614	NNS	denotes	agonists
T1519	4614-4616	,	denotes	,
T1520	4616-4619	CD	denotes	two
T1521	4620-4626	NNS	denotes	groups
T1522	4638-4641	CC	denotes	not
T1523	4642-4645	CD	denotes	one
T1524	4654-4659	NNS	denotes	genes
T1525	4646-4649	CC	denotes	but
T1526	4650-4653	CD	denotes	two
T1527	4659-4661	,	denotes	,
T1528	4661-4666	NN	denotes	ABCG5
T1529	4667-4670	CC	denotes	and
T1530	4671-4676	NN	denotes	ABCG8
T1531	4676-4678	,	denotes	,
T1532	4678-4683	WDT	denotes	which
T1533	4684-4690	VBP	denotes	encode
T1534	4691-4694	DT	denotes	the
T1535	4704-4712	NN	denotes	sterolin
T1536	4695-4703	NN	denotes	proteins
T1537	4712-4713	HYPH	denotes	-
T1538	4713-4714	CD	denotes	1
T1539	4715-4718	CC	denotes	and
T1540	4719-4727	NN	denotes	sterolin
T1541	4727-4728	HYPH	denotes	-
T1542	4728-4729	CD	denotes	2
T1543	4729-4731	,	denotes	,
T1544	4731-4740	NNS	denotes	mutations
T1545	4750-4755	VBP	denotes	cause
T1546	4741-4743	IN	denotes	of
T1547	4744-4749	WDT	denotes	which
T1548	4756-4770	NN	denotes	sitosterolemia
T1549	4771-4772	-LRB-	denotes	[
T1550	4772-4774	CD	denotes	14
T1551	4774-4775	SYM	denotes	-
T1552	4775-4777	CD	denotes	16
T1553	4777-4778	-RRB-	denotes	]
T1554	4778-4779	.	denotes	.
T1555	4779-4914	sentence	denotes	Interestingly, complete mutation in either ABCG5 alone or ABCG8 alone is not only necessary, but sufficient to cause the disease [14].
T1556	4780-4793	RB	denotes	Interestingly
T1557	4850-4852	VBZ	denotes	is
T1558	4793-4795	,	denotes	,
T1559	4795-4803	JJ	denotes	complete
T1560	4804-4812	NN	denotes	mutation
T1561	4813-4815	IN	denotes	in
T1562	4816-4822	CC	denotes	either
T1563	4823-4828	NN	denotes	ABCG5
T1564	4829-4834	RB	denotes	alone
T1565	4835-4837	CC	denotes	or
T1566	4838-4843	NN	denotes	ABCG8
T1567	4844-4849	RB	denotes	alone
T1568	4853-4856	RB	denotes	not
T1569	4862-4871	JJ	denotes	necessary
T1570	4857-4861	RB	denotes	only
T1571	4871-4873	,	denotes	,
T1572	4873-4876	CC	denotes	but
T1573	4877-4887	JJ	denotes	sufficient
T1574	4888-4890	TO	denotes	to
T1575	4891-4896	VB	denotes	cause
T1576	4897-4900	DT	denotes	the
T1577	4901-4908	NN	denotes	disease
T1578	4909-4910	-LRB-	denotes	[
T1579	4910-4912	CD	denotes	14
T1580	4912-4913	-RRB-	denotes	]
T1581	4913-4914	.	denotes	.
T1582	4914-5004	sentence	denotes	To date, no patient with sitosterolemia has been identified with mutations in both genes.
T1583	4915-4917	IN	denotes	To
T1584	4964-4974	VBN	denotes	identified
T1585	4918-4922	NN	denotes	date
T1586	4922-4924	,	denotes	,
T1587	4924-4926	DT	denotes	no
T1588	4927-4934	NN	denotes	patient
T1589	4935-4939	IN	denotes	with
T1590	4940-4954	NN	denotes	sitosterolemia
T1591	4955-4958	VBZ	denotes	has
T1592	4959-4963	VBN	denotes	been
T1593	4975-4979	IN	denotes	with
T1594	4980-4989	NNS	denotes	mutations
T1595	4990-4992	IN	denotes	in
T1596	4993-4997	DT	denotes	both
T1597	4998-5003	NNS	denotes	genes
T1598	5003-5004	.	denotes	.
T1599	5004-5170	sentence	denotes	Based upon these data, and the similarity of the clinical and biochemical phenotype, sterolin-1 and sterolin-2 are hypothesized to function as obligate heterodimers.
T1600	5005-5010	VBN	denotes	Based
T1601	5120-5132	VBN	denotes	hypothesized
T1602	5011-5015	IN	denotes	upon
T1603	5016-5021	DT	denotes	these
T1604	5022-5026	NNS	denotes	data
T1605	5026-5028	,	denotes	,
T1606	5028-5031	CC	denotes	and
T1607	5032-5035	DT	denotes	the
T1608	5036-5046	NN	denotes	similarity
T1609	5047-5049	IN	denotes	of
T1610	5050-5053	DT	denotes	the
T1611	5079-5088	NN	denotes	phenotype
T1612	5054-5062	JJ	denotes	clinical
T1613	5063-5066	CC	denotes	and
T1614	5067-5078	JJ	denotes	biochemical
T1615	5088-5090	,	denotes	,
T1616	5090-5098	NN	denotes	sterolin
T1617	5098-5099	HYPH	denotes	-
T1618	5099-5100	CD	denotes	1
T1619	5101-5104	CC	denotes	and
T1620	5105-5113	NN	denotes	sterolin
T1621	5113-5114	HYPH	denotes	-
T1622	5114-5115	CD	denotes	2
T1623	5116-5119	VBP	denotes	are
T1624	5133-5135	TO	denotes	to
T1625	5136-5144	VB	denotes	function
T1626	5145-5147	IN	denotes	as
T1627	5148-5156	JJ	denotes	obligate
T1628	5157-5169	NNS	denotes	heterodimers
T1629	5169-5170	.	denotes	.
T1630	5170-5382	sentence	denotes	This is also supported by the fact that each of these proteins is a 'half-transporter', containing six transmembrane domains, and not the classical 12 transmembrane characteristic of functional ABC transporters.
T1631	5171-5175	DT	denotes	This
T1632	5184-5193	VBN	denotes	supported
T1633	5176-5178	VBZ	denotes	is
T1634	5179-5183	RB	denotes	also
T1635	5194-5196	IN	denotes	by
T1636	5197-5200	DT	denotes	the
T1637	5201-5205	NN	denotes	fact
T1638	5206-5210	IN	denotes	that
T1639	5234-5236	VBZ	denotes	is
T1640	5211-5215	DT	denotes	each
T1641	5216-5218	IN	denotes	of
T1642	5219-5224	DT	denotes	these
T1643	5225-5233	NN	denotes	proteins
T1644	5237-5238	DT	denotes	a
T1645	5245-5256	NN	denotes	transporter
T1646	5239-5240	``	denotes	'
T1647	5240-5244	NN	denotes	half
T1648	5244-5245	HYPH	denotes	-
T1649	5256-5257	''	denotes	'
T1650	5257-5259	,	denotes	,
T1651	5259-5269	VBG	denotes	containing
T1652	5270-5273	CD	denotes	six
T1653	5288-5295	NNS	denotes	domains
T1654	5274-5287	NN	denotes	transmembrane
T1655	5295-5297	,	denotes	,
T1656	5297-5300	CC	denotes	and
T1657	5301-5304	RB	denotes	not
T1658	5305-5308	DT	denotes	the
T1659	5322-5335	NN	denotes	transmembrane
T1660	5309-5318	JJ	denotes	classical
T1661	5319-5321	CD	denotes	12
T1662	5336-5350	JJ	denotes	characteristic
T1663	5351-5353	IN	denotes	of
T1664	5354-5364	JJ	denotes	functional
T1665	5369-5381	NNS	denotes	transporters
T1666	5365-5368	NN	denotes	ABC
T1667	5381-5382	.	denotes	.
T1668	5382-5674	sentence	denotes	The exact function of these two proteins still remains unknown but recent studies have shown that overexpression of ABCG5/ABCG8 in a transgenic mouse model results in an increased biliary cholesterol concentration and a 50% reduction in the fractional absorption of dietary cholesterol [17].
T1669	5383-5386	DT	denotes	The
T1670	5393-5401	NN	denotes	function
T1671	5387-5392	JJ	denotes	exact
T1672	5430-5437	VBZ	denotes	remains
T1673	5402-5404	IN	denotes	of
T1674	5405-5410	DT	denotes	these
T1675	5415-5423	NN	denotes	proteins
T1676	5411-5414	CD	denotes	two
T1677	5424-5429	RB	denotes	still
T1678	5438-5445	JJ	denotes	unknown
T1679	5446-5449	CC	denotes	but
T1680	5450-5456	JJ	denotes	recent
T1681	5457-5464	NNS	denotes	studies
T1682	5470-5475	VBN	denotes	shown
T1683	5465-5469	VBP	denotes	have
T1684	5476-5480	IN	denotes	that
T1685	5539-5546	VBZ	denotes	results
T1686	5481-5495	NN	denotes	overexpression
T1687	5496-5498	IN	denotes	of
T1688	5499-5504	NN	denotes	ABCG5
T1689	5505-5510	NN	denotes	ABCG8
T1690	5504-5505	HYPH	denotes	/
T1691	5511-5513	IN	denotes	in
T1692	5514-5515	DT	denotes	a
T1693	5533-5538	NN	denotes	model
T1694	5516-5526	JJ	denotes	transgenic
T1695	5527-5532	NN	denotes	mouse
T1696	5547-5549	IN	denotes	in
T1697	5550-5552	DT	denotes	an
T1698	5583-5596	NN	denotes	concentration
T1699	5553-5562	VBN	denotes	increased
T1700	5563-5570	JJ	denotes	biliary
T1701	5571-5582	NN	denotes	cholesterol
T1702	5597-5600	CC	denotes	and
T1703	5601-5602	DT	denotes	a
T1704	5607-5616	NN	denotes	reduction
T1705	5603-5605	CD	denotes	50
T1706	5605-5606	NN	denotes	%
T1707	5617-5619	IN	denotes	in
T1708	5620-5623	DT	denotes	the
T1709	5635-5645	NN	denotes	absorption
T1710	5624-5634	JJ	denotes	fractional
T1711	5646-5648	IN	denotes	of
T1712	5649-5656	JJ	denotes	dietary
T1713	5657-5668	NN	denotes	cholesterol
T1714	5669-5670	-LRB-	denotes	[
T1715	5670-5672	CD	denotes	17
T1716	5672-5673	-RRB-	denotes	]
T1717	5673-5674	.	denotes	.
T1718	5674-5792	sentence	denotes	It has been suggested that ABCG5 and ABCG8 act as mutual chaperones for both maturation and correct apical targeting.
T1719	5675-5677	PRP	denotes	It
T1720	5687-5696	VBN	denotes	suggested
T1721	5678-5681	VBZ	denotes	has
T1722	5682-5686	VBN	denotes	been
T1723	5697-5701	IN	denotes	that
T1724	5718-5721	VBP	denotes	act
T1725	5702-5707	NN	denotes	ABCG5
T1726	5708-5711	CC	denotes	and
T1727	5712-5717	NN	denotes	ABCG8
T1728	5722-5724	IN	denotes	as
T1729	5725-5731	JJ	denotes	mutual
T1730	5732-5742	NNS	denotes	chaperones
T1731	5743-5746	IN	denotes	for
T1732	5747-5751	CC	denotes	both
T1733	5752-5762	NN	denotes	maturation
T1734	5763-5766	CC	denotes	and
T1735	5767-5774	JJ	denotes	correct
T1736	5782-5791	NN	denotes	targeting
T1737	5775-5781	JJ	denotes	apical
T1738	5791-5792	.	denotes	.
T1739	5792-5940	sentence	denotes	Co-expression of both was required for apical trafficking in a polarized cell line, as well in vivo using Abcg5/Abcg8 double-knockout mice [18,19].
T1740	5793-5806	NN	denotes	Co-expression
T1741	5819-5827	VBN	denotes	required
T1742	5807-5809	IN	denotes	of
T1743	5810-5814	DT	denotes	both
T1744	5815-5818	VBD	denotes	was
T1745	5828-5831	IN	denotes	for
T1746	5832-5838	JJ	denotes	apical
T1747	5839-5850	NN	denotes	trafficking
T1748	5851-5853	IN	denotes	in
T1749	5854-5855	DT	denotes	a
T1750	5871-5875	NN	denotes	line
T1751	5856-5865	VBN	denotes	polarized
T1752	5866-5870	NN	denotes	cell
T1753	5875-5877	,	denotes	,
T1754	5877-5879	RB	denotes	as
T1755	5880-5884	RB	denotes	well
T1756	5885-5887	FW	denotes	in
T1757	5888-5892	FW	denotes	vivo
T1758	5893-5898	VBG	denotes	using
T1759	5899-5904	NN	denotes	Abcg5
T1760	5905-5910	NN	denotes	Abcg8
T1761	5904-5905	HYPH	denotes	/
T1762	5918-5926	NN	denotes	knockout
T1763	5911-5917	RB	denotes	double
T1764	5917-5918	HYPH	denotes	-
T1765	5927-5931	NNS	denotes	mice
T1766	5932-5933	-LRB-	denotes	[
T1767	5936-5938	CD	denotes	19
T1768	5933-5935	CD	denotes	18
T1769	5935-5936	,	denotes	,
T1770	5938-5939	-RRB-	denotes	]
T1771	5939-5940	.	denotes	.
T1772	5940-6125	sentence	denotes	However, no functional assay has yet been developed to demonstrate whether or not the two half-transporters function as homo- or heterodimers and whether they selectively pump sterols.
T1773	5941-5948	RB	denotes	However
T1774	5983-5992	VBN	denotes	developed
T1775	5948-5950	,	denotes	,
T1776	5950-5952	DT	denotes	no
T1777	5964-5969	NN	denotes	assay
T1778	5953-5963	JJ	denotes	functional
T1779	5970-5973	VBZ	denotes	has
T1780	5974-5977	RB	denotes	yet
T1781	5978-5982	VBN	denotes	been
T1782	5993-5995	TO	denotes	to
T1783	5996-6007	VB	denotes	demonstrate
T1784	6008-6015	IN	denotes	whether
T1785	6049-6057	VBP	denotes	function
T1786	6016-6018	CC	denotes	or
T1787	6019-6022	RB	denotes	not
T1788	6023-6026	DT	denotes	the
T1789	6036-6048	NNS	denotes	transporters
T1790	6027-6030	CD	denotes	two
T1791	6031-6035	NN	denotes	half
T1792	6035-6036	HYPH	denotes	-
T1793	6058-6060	IN	denotes	as
T1794	6061-6065	AFX	denotes	homo
T1795	6070-6082	NNS	denotes	heterodimers
T1796	6065-6066	HYPH	denotes	-
T1797	6067-6069	CC	denotes	or
T1798	6083-6086	CC	denotes	and
T1799	6087-6094	IN	denotes	whether
T1800	6112-6116	VB	denotes	pump
T1801	6095-6099	PRP	denotes	they
T1802	6100-6111	RB	denotes	selectively
T1803	6117-6124	NNS	denotes	sterols
T1804	6124-6125	.	denotes	.
T1805	6125-6256	sentence	denotes	Likewise, the cellular localization of these two proteins has yet to be shown in a model deficient in either Abcg5 or Abcg8 alone.
T1806	6126-6134	RB	denotes	Likewise
T1807	6184-6187	VBZ	denotes	has
T1808	6134-6136	,	denotes	,
T1809	6136-6139	DT	denotes	the
T1810	6149-6161	NN	denotes	localization
T1811	6140-6148	JJ	denotes	cellular
T1812	6162-6164	IN	denotes	of
T1813	6165-6170	DT	denotes	these
T1814	6175-6183	NN	denotes	proteins
T1815	6171-6174	CD	denotes	two
T1816	6188-6191	RB	denotes	yet
T1817	6192-6194	TO	denotes	to
T1818	6198-6203	VBN	denotes	shown
T1819	6195-6197	VB	denotes	be
T1820	6204-6206	IN	denotes	in
T1821	6207-6208	DT	denotes	a
T1822	6209-6214	NN	denotes	model
T1823	6215-6224	JJ	denotes	deficient
T1824	6225-6227	IN	denotes	in
T1825	6228-6234	CC	denotes	either
T1826	6235-6240	NN	denotes	Abcg5
T1827	6241-6243	CC	denotes	or
T1828	6244-6249	NN	denotes	Abcg8
T1829	6250-6255	RB	denotes	alone
T1830	6255-6256	.	denotes	.
T1831	6256-6383	sentence	denotes	A mouse model disrupting both Abcg5 and Abcg8 simultaneously results in a phenotype similar to that of the human disease [20].
T1832	6257-6258	DT	denotes	A
T1833	6265-6270	NN	denotes	model
T1834	6259-6264	NN	denotes	mouse
T1835	6318-6325	VBZ	denotes	results
T1836	6271-6281	VBG	denotes	disrupting
T1837	6282-6286	CC	denotes	both
T1838	6287-6292	NN	denotes	Abcg5
T1839	6293-6296	CC	denotes	and
T1840	6297-6302	NN	denotes	Abcg8
T1841	6303-6317	RB	denotes	simultaneously
T1842	6326-6328	IN	denotes	in
T1843	6329-6330	DT	denotes	a
T1844	6331-6340	NN	denotes	phenotype
T1845	6341-6348	JJ	denotes	similar
T1846	6349-6351	IN	denotes	to
T1847	6352-6356	DT	denotes	that
T1848	6357-6359	IN	denotes	of
T1849	6360-6363	DT	denotes	the
T1850	6370-6377	NN	denotes	disease
T1851	6364-6369	JJ	denotes	human
T1852	6378-6379	-LRB-	denotes	[
T1853	6379-6381	CD	denotes	20
T1854	6381-6382	-RRB-	denotes	]
T1855	6382-6383	.	denotes	.
T1856	6383-6530	sentence	denotes	However, it is important to point out that the human disease is caused by complete mutation in one or the other gene, but not both simultaneously.
T1857	6384-6391	RB	denotes	However
T1858	6396-6398	VBZ	denotes	is
T1859	6391-6393	,	denotes	,
T1860	6393-6395	PRP	denotes	it
T1861	6399-6408	JJ	denotes	important
T1862	6409-6411	TO	denotes	to
T1863	6412-6417	VB	denotes	point
T1864	6418-6421	RP	denotes	out
T1865	6422-6426	IN	denotes	that
T1866	6448-6454	VBN	denotes	caused
T1867	6427-6430	DT	denotes	the
T1868	6437-6444	NN	denotes	disease
T1869	6431-6436	JJ	denotes	human
T1870	6445-6447	VBZ	denotes	is
T1871	6455-6457	IN	denotes	by
T1872	6458-6466	JJ	denotes	complete
T1873	6467-6475	NN	denotes	mutation
T1874	6476-6478	IN	denotes	in
T1875	6479-6482	CD	denotes	one
T1876	6483-6485	CC	denotes	or
T1877	6486-6489	DT	denotes	the
T1878	6490-6495	JJ	denotes	other
T1879	6496-6500	NN	denotes	gene
T1880	6500-6502	,	denotes	,
T1881	6502-6505	CC	denotes	but
T1882	6506-6509	RB	denotes	not
T1883	6510-6514	DT	denotes	both
T1884	6515-6529	RB	denotes	simultaneously
T1885	6529-6530	.	denotes	.
T1886	6530-6772	sentence	denotes	We hypothesized that disruption of only one of the half-transporters would result in sitosterolemia and selective loss of either sterolin may show a differential effect on the biliary secretion of sterols or intestinal absorption of sterols.
T1887	6531-6533	PRP	denotes	We
T1888	6534-6546	VBD	denotes	hypothesized
T1889	6547-6551	IN	denotes	that
T1890	6606-6612	VB	denotes	result
T1891	6552-6562	NN	denotes	disruption
T1892	6563-6565	IN	denotes	of
T1893	6566-6570	RB	denotes	only
T1894	6571-6574	CD	denotes	one
T1895	6575-6577	IN	denotes	of
T1896	6578-6581	DT	denotes	the
T1897	6587-6599	NNS	denotes	transporters
T1898	6582-6586	NN	denotes	half
T1899	6586-6587	HYPH	denotes	-
T1900	6600-6605	MD	denotes	would
T1901	6613-6615	IN	denotes	in
T1902	6616-6630	NN	denotes	sitosterolemia
T1903	6631-6634	CC	denotes	and
T1904	6635-6644	JJ	denotes	selective
T1905	6645-6649	NN	denotes	loss
T1906	6673-6677	VB	denotes	show
T1907	6650-6652	IN	denotes	of
T1908	6653-6659	DT	denotes	either
T1909	6660-6668	NN	denotes	sterolin
T1910	6669-6672	MD	denotes	may
T1911	6678-6679	DT	denotes	a
T1912	6693-6699	NN	denotes	effect
T1913	6680-6692	JJ	denotes	differential
T1914	6700-6702	IN	denotes	on
T1915	6703-6706	DT	denotes	the
T1916	6715-6724	NN	denotes	secretion
T1917	6707-6714	JJ	denotes	biliary
T1918	6725-6727	IN	denotes	of
T1919	6728-6735	NNS	denotes	sterols
T1920	6736-6738	CC	denotes	or
T1921	6739-6749	JJ	denotes	intestinal
T1922	6750-6760	NN	denotes	absorption
T1923	6761-6763	IN	denotes	of
T1924	6764-6771	NNS	denotes	sterols
T1925	6771-6772	.	denotes	.
T1926	6772-6869	sentence	denotes	These studies are difficult to test in humans, as the target organs are relatively inaccessible.
T1927	6773-6778	DT	denotes	These
T1928	6779-6786	NNS	denotes	studies
T1929	6787-6790	VBP	denotes	are
T1930	6791-6800	JJ	denotes	difficult
T1931	6801-6803	TO	denotes	to
T1932	6804-6808	VB	denotes	test
T1933	6809-6811	IN	denotes	in
T1934	6812-6818	NNS	denotes	humans
T1935	6818-6820	,	denotes	,
T1936	6820-6822	IN	denotes	as
T1937	6841-6844	VBP	denotes	are
T1938	6823-6826	DT	denotes	the
T1939	6834-6840	NNS	denotes	organs
T1940	6827-6833	NN	denotes	target
T1941	6845-6855	RB	denotes	relatively
T1942	6856-6868	JJ	denotes	inaccessible
T1943	6868-6869	.	denotes	.
T1944	6869-6975	sentence	denotes	In order to test this hypothesis, we report a mouse model that has a selective genetic mutation of Abcg8.
T1945	6870-6872	IN	denotes	In
T1946	6907-6913	VBP	denotes	report
T1947	6873-6878	NN	denotes	order
T1948	6879-6881	TO	denotes	to
T1949	6882-6886	VB	denotes	test
T1950	6887-6891	DT	denotes	this
T1951	6892-6902	NN	denotes	hypothesis
T1952	6902-6904	,	denotes	,
T1953	6904-6906	PRP	denotes	we
T1954	6914-6915	DT	denotes	a
T1955	6922-6927	NN	denotes	model
T1956	6916-6921	NN	denotes	mouse
T1957	6928-6932	WDT	denotes	that
T1958	6933-6936	VBZ	denotes	has
T1959	6937-6938	DT	denotes	a
T1960	6957-6965	NN	denotes	mutation
T1961	6939-6948	JJ	denotes	selective
T1962	6949-6956	JJ	denotes	genetic
T1963	6966-6968	IN	denotes	of
T1964	6969-6974	NN	denotes	Abcg8
T1965	6974-6975	.	denotes	.
T1966	6975-7079	sentence	denotes	Mice homozygous for Abcg8 loss exhibit many of the characteristics of the human disease sitosterolemia.
T1967	6976-6980	NNS	denotes	Mice
T1968	7007-7014	VBP	denotes	exhibit
T1969	6981-6991	JJ	denotes	homozygous
T1970	6992-6995	IN	denotes	for
T1971	6996-7001	NN	denotes	Abcg8
T1972	7002-7006	NN	denotes	loss
T1973	7015-7019	JJ	denotes	many
T1974	7020-7022	IN	denotes	of
T1975	7023-7026	DT	denotes	the
T1976	7027-7042	NNS	denotes	characteristics
T1977	7043-7045	IN	denotes	of
T1978	7046-7049	DT	denotes	the
T1979	7056-7063	NN	denotes	disease
T1980	7050-7055	JJ	denotes	human
T1981	7064-7078	NN	denotes	sitosterolemia
T1982	7078-7079	.	denotes	.
T1983	7079-7290	sentence	denotes	In addition, we report studies of biliary sterol secretion that show that despite forced secretion, Abcg8-deficient animals cannot secrete cholesterol into bile, although sitosterol secretion seemed unaffected.
T1984	7080-7082	IN	denotes	In
T1985	7096-7102	VBP	denotes	report
T1986	7083-7091	NN	denotes	addition
T1987	7091-7093	,	denotes	,
T1988	7093-7095	PRP	denotes	we
T1989	7103-7110	NNS	denotes	studies
T1990	7111-7113	IN	denotes	of
T1991	7114-7121	JJ	denotes	biliary
T1992	7129-7138	NN	denotes	secretion
T1993	7122-7128	NN	denotes	sterol
T1994	7139-7143	WDT	denotes	that
T1995	7144-7148	VBP	denotes	show
T1996	7149-7153	IN	denotes	that
T1997	7211-7218	VB	denotes	secrete
T1998	7154-7161	IN	denotes	despite
T1999	7162-7168	JJ	denotes	forced
T2000	7169-7178	NN	denotes	secretion
T2001	7178-7180	,	denotes	,
T2002	7180-7185	NN	denotes	Abcg8
T2003	7186-7195	JJ	denotes	deficient
T2004	7185-7186	HYPH	denotes	-
T2005	7196-7203	NNS	denotes	animals
T2006	7204-7207	MD	denotes	can
T2007	7207-7210	RB	denotes	not
T2008	7219-7230	NN	denotes	cholesterol
T2009	7231-7235	IN	denotes	into
T2010	7236-7240	NN	denotes	bile
T2011	7240-7242	,	denotes	,
T2012	7242-7250	IN	denotes	although
T2013	7272-7278	VBD	denotes	seemed
T2014	7251-7261	NN	denotes	sitosterol
T2015	7262-7271	NN	denotes	secretion
T2016	7279-7289	JJ	denotes	unaffected
T2017	7289-7290	.	denotes	.
T2220	7301-7310	NN	denotes	Targeting
T2221	7311-7317	NN	denotes	vector
T2222	7318-7330	NN	denotes	construction
T2223	7331-7334	CC	denotes	and
T2224	7335-7345	NN	denotes	generation
T2225	7346-7348	IN	denotes	of
T2226	7349-7357	NN	denotes	knockout
T2227	7358-7362	NNS	denotes	mice
T2228	7362-7617	sentence	denotes	A mouse genomic bacterial artificial chromosome (BAC) library (CitbCJ7, ES cell line/129Sv, Research Genetics, Inc., Huntsville, AL, USA) was screened by using primers designed from the sequences of mouse Abcg5 and Abcg8 cDNA as previously reported [21].
T2229	7363-7364	DT	denotes	A
T2230	7417-7424	NN	denotes	library
T2231	7365-7370	NN	denotes	mouse
T2232	7371-7378	JJ	denotes	genomic
T2233	7379-7388	JJ	denotes	bacterial
T2234	7400-7410	NN	denotes	chromosome
T2235	7389-7399	JJ	denotes	artificial
T2236	7411-7412	-LRB-	denotes	(
T2237	7412-7415	NN	denotes	BAC
T2238	7415-7416	-RRB-	denotes	)
T2239	7505-7513	VBN	denotes	screened
T2240	7425-7426	-LRB-	denotes	(
T2241	7480-7490	NNP	denotes	Huntsville
T2242	7426-7433	NN	denotes	CitbCJ7
T2243	7433-7435	,	denotes	,
T2244	7435-7437	NN	denotes	ES
T2245	7438-7442	NN	denotes	cell
T2246	7448-7453	NN	denotes	129Sv
T2247	7443-7447	NN	denotes	line
T2248	7447-7448	HYPH	denotes	/
T2249	7453-7455	,	denotes	,
T2250	7455-7463	NNP	denotes	Research
T2251	7464-7472	NNP	denotes	Genetics
T2252	7472-7474	,	denotes	,
T2253	7474-7478	NNP	denotes	Inc.
T2254	7478-7480	,	denotes	,
T2255	7490-7492	,	denotes	,
T2256	7492-7494	NNP	denotes	AL
T2257	7494-7496	,	denotes	,
T2258	7496-7499	NNP	denotes	USA
T2259	7499-7500	-RRB-	denotes	)
T2260	7501-7504	VBD	denotes	was
T2261	7514-7516	IN	denotes	by
T2262	7517-7522	VBG	denotes	using
T2263	7523-7530	NNS	denotes	primers
T2264	7531-7539	VBN	denotes	designed
T2265	7540-7544	IN	denotes	from
T2266	7545-7548	DT	denotes	the
T2267	7549-7558	NNS	denotes	sequences
T2268	7559-7561	IN	denotes	of
T2269	7562-7567	NN	denotes	mouse
T2270	7584-7588	NN	denotes	cDNA
T2271	7568-7573	NN	denotes	Abcg5
T2272	7574-7577	CC	denotes	and
T2273	7578-7583	NN	denotes	Abcg8
T2274	7589-7591	IN	denotes	as
T2275	7603-7611	VBN	denotes	reported
T2276	7592-7602	RB	denotes	previously
T2277	7612-7613	-LRB-	denotes	[
T2278	7613-7615	CD	denotes	21
T2279	7615-7616	-RRB-	denotes	]
T2280	7616-7617	.	denotes	.
T2281	7617-7704	sentence	denotes	A positive BAC clone was used as a template to amplify genomic DNA fragments of Abcg8.
T2282	7618-7619	DT	denotes	A
T2283	7633-7638	NN	denotes	clone
T2284	7620-7628	JJ	denotes	positive
T2285	7629-7632	NN	denotes	BAC
T2286	7643-7647	VBN	denotes	used
T2287	7639-7642	VBD	denotes	was
T2288	7648-7650	IN	denotes	as
T2289	7651-7652	DT	denotes	a
T2290	7653-7661	NN	denotes	template
T2291	7662-7664	TO	denotes	to
T2292	7665-7672	VB	denotes	amplify
T2293	7673-7680	JJ	denotes	genomic
T2294	7685-7694	NNS	denotes	fragments
T2295	7681-7684	NN	denotes	DNA
T2296	7695-7697	IN	denotes	of
T2297	7698-7703	NN	denotes	Abcg8
T2298	7703-7704	.	denotes	.
T2299	7704-7856	sentence	denotes	Long-fragment polymerase chain reaction (PCR) was performed using Expanded Long Template PCR system kit (Roche Applied Science, Indianapolis, IA, USA).
T2300	7705-7709	JJ	denotes	Long
T2301	7710-7718	NN	denotes	fragment
T2302	7709-7710	HYPH	denotes	-
T2303	7755-7764	VBN	denotes	performed
T2304	7719-7729	NN	denotes	polymerase
T2305	7736-7744	NN	denotes	reaction
T2306	7730-7735	NN	denotes	chain
T2307	7745-7746	-LRB-	denotes	(
T2308	7746-7749	NN	denotes	PCR
T2309	7749-7750	-RRB-	denotes	)
T2310	7751-7754	VBD	denotes	was
T2311	7765-7770	VBG	denotes	using
T2312	7771-7779	NN	denotes	Expanded
T2313	7798-7804	NN	denotes	system
T2314	7780-7784	JJ	denotes	Long
T2315	7794-7797	NN	denotes	PCR
T2316	7785-7793	NN	denotes	Template
T2317	7805-7808	NN	denotes	kit
T2318	7809-7810	-LRB-	denotes	(
T2319	7824-7831	NNP	denotes	Science
T2320	7810-7815	NNP	denotes	Roche
T2321	7816-7823	NNP	denotes	Applied
T2322	7831-7833	,	denotes	,
T2323	7833-7845	NNP	denotes	Indianapolis
T2324	7845-7847	,	denotes	,
T2325	7847-7849	NNP	denotes	IA
T2326	7849-7851	,	denotes	,
T2327	7851-7854	NNP	denotes	USA
T2328	7854-7855	-RRB-	denotes	)
T2329	7855-7856	.	denotes	.
T2330	7856-8027	sentence	denotes	An approximately 4.5 kb 'long-arm' genomic fragment containing partial exon 1 to partial exon 3 was inserted into the Pml I restriction-cloning site A of OSDUPDEL vector.
T2331	7857-7859	DT	denotes	An
T2332	7900-7908	NN	denotes	fragment
T2333	7860-7873	RB	denotes	approximately
T2334	7874-7877	CD	denotes	4.5
T2335	7878-7880	NN	denotes	kb
T2336	7881-7882	``	denotes	'
T2337	7882-7886	JJ	denotes	long
T2338	7887-7890	NN	denotes	arm
T2339	7886-7887	HYPH	denotes	-
T2340	7890-7891	''	denotes	'
T2341	7892-7899	JJ	denotes	genomic
T2342	7957-7965	VBN	denotes	inserted
T2343	7909-7919	VBG	denotes	containing
T2344	7920-7927	JJ	denotes	partial
T2345	7928-7932	NN	denotes	exon
T2346	7933-7934	CD	denotes	1
T2347	7935-7937	IN	denotes	to
T2348	7938-7945	JJ	denotes	partial
T2349	7946-7950	NN	denotes	exon
T2350	7951-7952	CD	denotes	3
T2351	7953-7956	VBD	denotes	was
T2352	7966-7970	IN	denotes	into
T2353	7971-7974	DT	denotes	the
T2354	8006-8007	NN	denotes	A
T2355	7975-7978	NN	denotes	Pml
T2356	7979-7980	CD	denotes	I
T2357	7981-7992	NN	denotes	restriction
T2358	7993-8000	VBG	denotes	cloning
T2359	7992-7993	HYPH	denotes	-
T2360	8001-8005	NN	denotes	site
T2361	8008-8010	IN	denotes	of
T2362	8011-8019	NN	denotes	OSDUPDEL
T2363	8020-8026	NN	denotes	vector
T2364	8026-8027	.	denotes	.
T2365	8027-8157	sentence	denotes	The 'short-arm' genomic fragment, containing partial exon 4 to partial exon 6, was cloned into the Not I-Kpn I of cloning site B.
T2366	8028-8031	DT	denotes	The
T2367	8052-8060	NN	denotes	fragment
T2368	8032-8033	``	denotes	'
T2369	8033-8038	NN	denotes	short
T2370	8039-8042	NN	denotes	arm
T2371	8038-8039	HYPH	denotes	-
T2372	8042-8043	''	denotes	'
T2373	8044-8051	JJ	denotes	genomic
T2374	8111-8117	VBN	denotes	cloned
T2375	8060-8062	,	denotes	,
T2376	8062-8072	VBG	denotes	containing
T2377	8073-8080	JJ	denotes	partial
T2378	8081-8085	NN	denotes	exon
T2379	8086-8087	CD	denotes	4
T2380	8088-8090	IN	denotes	to
T2381	8091-8098	JJ	denotes	partial
T2382	8099-8103	NN	denotes	exon
T2383	8104-8105	CD	denotes	6
T2384	8105-8107	,	denotes	,
T2385	8107-8110	VBD	denotes	was
T2386	8118-8122	IN	denotes	into
T2387	8123-8126	DT	denotes	the
T2388	8133-8136	NN	denotes	Kpn
T2389	8127-8130	NN	denotes	Not
T2390	8131-8132	NN	denotes	I
T2391	8132-8133	HYPH	denotes	-
T2392	8137-8138	CD	denotes	I
T2393	8139-8141	IN	denotes	of
T2394	8142-8149	VBG	denotes	cloning
T2395	8155-8156	NN	denotes	B
T2396	8150-8154	NN	denotes	site
T2397	8156-8157	.	denotes	.
T2398	8157-8363	sentence	denotes	Homologous targeting would result in complete intron 3, partial exon 3 and partial exon 4 replacement by the neomycin-resistance cassette, resulting in the disruption of the ABC Walker A motif (Figure 1a).
T2399	8158-8168	JJ	denotes	Homologous
T2400	8169-8178	NN	denotes	targeting
T2401	8185-8191	VB	denotes	result
T2402	8179-8184	MD	denotes	would
T2403	8192-8194	IN	denotes	in
T2404	8195-8203	JJ	denotes	complete
T2405	8204-8210	NN	denotes	intron
T2406	8211-8212	CD	denotes	3
T2407	8212-8214	,	denotes	,
T2408	8214-8221	JJ	denotes	partial
T2409	8222-8226	NN	denotes	exon
T2410	8227-8228	CD	denotes	3
T2411	8229-8232	CC	denotes	and
T2412	8233-8240	JJ	denotes	partial
T2413	8241-8245	NN	denotes	exon
T2414	8248-8259	NN	denotes	replacement
T2415	8246-8247	CD	denotes	4
T2416	8260-8262	IN	denotes	by
T2417	8263-8266	DT	denotes	the
T2418	8287-8295	NN	denotes	cassette
T2419	8267-8275	NN	denotes	neomycin
T2420	8275-8276	HYPH	denotes	-
T2421	8276-8286	JJ	denotes	resistance
T2422	8295-8297	,	denotes	,
T2423	8297-8306	VBG	denotes	resulting
T2424	8307-8309	IN	denotes	in
T2425	8310-8313	DT	denotes	the
T2426	8314-8324	NN	denotes	disruption
T2427	8325-8327	IN	denotes	of
T2428	8328-8331	DT	denotes	the
T2429	8345-8350	NN	denotes	motif
T2430	8332-8335	NN	denotes	ABC
T2431	8343-8344	NN	denotes	A
T2432	8336-8342	NN	denotes	Walker
T2433	8351-8352	-LRB-	denotes	(
T2434	8359-8361	NN	denotes	1a
T2435	8352-8358	NN	denotes	Figure
T2436	8361-8362	-RRB-	denotes	)
T2437	8362-8363	.	denotes	.
T2438	8363-8510	sentence	denotes	ES cells (129/SvEvTac-cell line) were electroporated with the linearized targeting vector DNA and cultured on sub-confluent embryonic fibroblasts.
T2439	8364-8366	NN	denotes	ES
T2440	8367-8372	NNS	denotes	cells
T2441	8402-8416	VBN	denotes	electroporated
T2442	8373-8374	-LRB-	denotes	(
T2443	8391-8395	NN	denotes	line
T2444	8374-8377	CD	denotes	129
T2445	8378-8385	NN	denotes	SvEvTac
T2446	8377-8378	HYPH	denotes	/
T2447	8385-8386	HYPH	denotes	-
T2448	8386-8390	NN	denotes	cell
T2449	8395-8396	-RRB-	denotes	)
T2450	8397-8401	VBD	denotes	were
T2451	8417-8421	IN	denotes	with
T2452	8422-8425	DT	denotes	the
T2453	8454-8457	NN	denotes	DNA
T2454	8426-8436	VBN	denotes	linearized
T2455	8447-8453	NN	denotes	vector
T2456	8437-8446	NN	denotes	targeting
T2457	8458-8461	CC	denotes	and
T2458	8462-8470	VBN	denotes	cultured
T2459	8471-8473	IN	denotes	on
T2460	8474-8487	JJ	denotes	sub-confluent
T2461	8498-8509	NNS	denotes	fibroblasts
T2462	8488-8497	JJ	denotes	embryonic
T2463	8509-8510	.	denotes	.
T2464	8510-8646	sentence	denotes	Transfected cell colonies were selected by culturing cells in medium containing 200 μg/mL G418 (Life Technologies, Rockville, MD, USA).
T2465	8511-8522	VBN	denotes	Transfected
T2466	8528-8536	NNS	denotes	colonies
T2467	8523-8527	NN	denotes	cell
T2468	8542-8550	VBN	denotes	selected
T2469	8537-8541	VBD	denotes	were
T2470	8551-8553	IN	denotes	by
T2471	8554-8563	VBG	denotes	culturing
T2472	8564-8569	NNS	denotes	cells
T2473	8570-8572	IN	denotes	in
T2474	8573-8579	NN	denotes	medium
T2475	8580-8590	VBG	denotes	containing
T2476	8591-8594	CD	denotes	200
T2477	8595-8597	NN	denotes	μg
T2478	8601-8605	NN	denotes	G418
T2479	8597-8598	SYM	denotes	/
T2480	8598-8600	NN	denotes	mL
T2481	8606-8607	-LRB-	denotes	(
T2482	8612-8624	NNP	denotes	Technologies
T2483	8607-8611	NNP	denotes	Life
T2484	8624-8626	,	denotes	,
T2485	8626-8635	NNP	denotes	Rockville
T2486	8635-8637	,	denotes	,
T2487	8637-8639	NNP	denotes	MD
T2488	8639-8641	,	denotes	,
T2489	8641-8644	NNP	denotes	USA
T2490	8644-8645	-RRB-	denotes	)
T2491	8645-8646	.	denotes	.
T2492	8646-8920	sentence	denotes	The ES cells were screened for homologous recombination by PCR with the forward primer, neoF (5'-GGGTCGTTTGTTCGGATCAA-3') from neo cassette, and reverse primer intron 6R (5'-ACCAGTTGGTCCTAGCTCGA-3'), which is located outside the targeting construct in mouse Abcg8 intron 6.
T2493	8647-8650	DT	denotes	The
T2494	8654-8659	NNS	denotes	cells
T2495	8651-8653	NN	denotes	ES
T2496	8665-8673	VBN	denotes	screened
T2497	8660-8664	VBD	denotes	were
T2498	8674-8677	IN	denotes	for
T2499	8678-8688	JJ	denotes	homologous
T2500	8689-8702	NN	denotes	recombination
T2501	8703-8705	IN	denotes	by
T2502	8706-8709	NN	denotes	PCR
T2503	8710-8714	IN	denotes	with
T2504	8715-8718	DT	denotes	the
T2505	8727-8733	NN	denotes	primer
T2506	8719-8726	JJ	denotes	forward
T2507	8733-8735	,	denotes	,
T2508	8735-8739	NN	denotes	neoF
T2509	8740-8741	-LRB-	denotes	(
T2510	8741-8742	CD	denotes	5
T2511	8744-8764	NN	denotes	GGGTCGTTTGTTCGGATCAA
T2512	8742-8743	SYM	denotes	'
T2513	8743-8744	HYPH	denotes	-
T2514	8764-8765	HYPH	denotes	-
T2515	8765-8766	CD	denotes	3
T2516	8766-8767	SYM	denotes	'
T2517	8767-8768	-RRB-	denotes	)
T2518	8769-8773	IN	denotes	from
T2519	8774-8777	NN	denotes	neo
T2520	8778-8786	NN	denotes	cassette
T2521	8786-8788	,	denotes	,
T2522	8788-8791	CC	denotes	and
T2523	8792-8799	JJ	denotes	reverse
T2524	8800-8806	NN	denotes	primer
T2525	8814-8816	NN	denotes	6R
T2526	8807-8813	NN	denotes	intron
T2527	8817-8818	-LRB-	denotes	(
T2528	8818-8819	CD	denotes	5
T2529	8821-8841	NN	denotes	ACCAGTTGGTCCTAGCTCGA
T2530	8819-8820	SYM	denotes	'
T2531	8820-8821	HYPH	denotes	-
T2532	8841-8842	HYPH	denotes	-
T2533	8842-8843	CD	denotes	3
T2534	8843-8844	SYM	denotes	'
T2535	8844-8845	-RRB-	denotes	)
T2536	8845-8847	,	denotes	,
T2537	8847-8852	WDT	denotes	which
T2538	8856-8863	VBN	denotes	located
T2539	8853-8855	VBZ	denotes	is
T2540	8864-8871	IN	denotes	outside
T2541	8872-8875	DT	denotes	the
T2542	8886-8895	NN	denotes	construct
T2543	8876-8885	VBG	denotes	targeting
T2544	8896-8898	IN	denotes	in
T2545	8899-8904	NN	denotes	mouse
T2546	8911-8917	NN	denotes	intron
T2547	8905-8910	NN	denotes	Abcg8
T2548	8918-8919	CD	denotes	6
T2549	8919-8920	.	denotes	.
T2550	8920-9124	sentence	denotes	Positively targeted ES cell clones were confirmed by Southern blotting, using ES cell DNA digested by BamHI and a probe comprising of a 562 bp PCR fragment from partial intron 2 and exon 3 of Abcg8 gene.
T2551	8921-8931	RB	denotes	Positively
T2552	8932-8940	VBN	denotes	targeted
T2553	8949-8955	NNS	denotes	clones
T2554	8941-8943	NN	denotes	ES
T2555	8944-8948	NN	denotes	cell
T2556	8961-8970	VBN	denotes	confirmed
T2557	8956-8960	VBD	denotes	were
T2558	8971-8973	IN	denotes	by
T2559	8974-8982	NNP	denotes	Southern
T2560	8983-8991	NN	denotes	blotting
T2561	8991-8993	,	denotes	,
T2562	8993-8998	VBG	denotes	using
T2563	8999-9001	NN	denotes	ES
T2564	9002-9006	NN	denotes	cell
T2565	9007-9010	NN	denotes	DNA
T2566	9011-9019	VBN	denotes	digested
T2567	9020-9022	IN	denotes	by
T2568	9023-9028	NN	denotes	BamHI
T2569	9029-9032	CC	denotes	and
T2570	9033-9034	DT	denotes	a
T2571	9035-9040	NN	denotes	probe
T2572	9041-9051	VBZ	denotes	comprising
T2573	9052-9054	IN	denotes	of
T2574	9055-9056	DT	denotes	a
T2575	9068-9076	NN	denotes	fragment
T2576	9057-9060	CD	denotes	562
T2577	9061-9063	NN	denotes	bp
T2578	9064-9067	NN	denotes	PCR
T2579	9077-9081	IN	denotes	from
T2580	9082-9089	JJ	denotes	partial
T2581	9090-9096	NN	denotes	intron
T2582	9097-9098	CD	denotes	2
T2583	9099-9102	CC	denotes	and
T2584	9103-9107	NN	denotes	exon
T2585	9108-9109	CD	denotes	3
T2586	9110-9112	IN	denotes	of
T2587	9113-9118	NN	denotes	Abcg8
T2588	9119-9123	NN	denotes	gene
T2589	9123-9124	.	denotes	.
T2590	9124-9245	sentence	denotes	Positively targeted ES cells were microinjected into C57BL6 blastocysts and transplanted into pseudopregnant recipients.
T2591	9125-9135	RB	denotes	Positively
T2592	9136-9144	VBN	denotes	targeted
T2593	9148-9153	NNS	denotes	cells
T2594	9145-9147	NN	denotes	ES
T2595	9159-9172	VBN	denotes	microinjected
T2596	9154-9158	VBD	denotes	were
T2597	9173-9177	IN	denotes	into
T2598	9178-9184	NN	denotes	C57BL6
T2599	9185-9196	NNS	denotes	blastocysts
T2600	9197-9200	CC	denotes	and
T2601	9201-9213	VBN	denotes	transplanted
T2602	9214-9218	IN	denotes	into
T2603	9219-9233	JJ	denotes	pseudopregnant
T2604	9234-9244	NNS	denotes	recipients
T2605	9244-9245	.	denotes	.
T2606	9245-9441	sentence	denotes	Five highly chimeric mice (agouti coat color, three males and two females) were isolated and bred with C57BL/6J mice to generate germ-line transmission heterozygous mice of Abcg8 gene disruption.
T2607	9246-9250	CD	denotes	Five
T2608	9267-9271	NNS	denotes	mice
T2609	9251-9257	RB	denotes	highly
T2610	9258-9266	JJ	denotes	chimeric
T2611	9326-9334	VBN	denotes	isolated
T2612	9272-9273	-LRB-	denotes	(
T2613	9298-9303	NNS	denotes	males
T2614	9273-9279	JJ	denotes	agouti
T2615	9285-9290	NN	denotes	color
T2616	9280-9284	NN	denotes	coat
T2617	9290-9292	,	denotes	,
T2618	9292-9297	CD	denotes	three
T2619	9304-9307	CC	denotes	and
T2620	9308-9311	CD	denotes	two
T2621	9312-9319	NNS	denotes	females
T2622	9319-9320	-RRB-	denotes	)
T2623	9321-9325	VBD	denotes	were
T2624	9335-9338	CC	denotes	and
T2625	9339-9343	VBN	denotes	bred
T2626	9344-9348	IN	denotes	with
T2627	9349-9354	NN	denotes	C57BL
T2628	9355-9357	NN	denotes	6J
T2629	9354-9355	HYPH	denotes	/
T2630	9358-9362	NNS	denotes	mice
T2631	9363-9365	TO	denotes	to
T2632	9366-9374	VB	denotes	generate
T2633	9375-9379	NN	denotes	germ
T2634	9380-9384	NN	denotes	line
T2635	9379-9380	HYPH	denotes	-
T2636	9385-9397	NN	denotes	transmission
T2637	9398-9410	JJ	denotes	heterozygous
T2638	9411-9415	NNS	denotes	mice
T2639	9416-9418	IN	denotes	of
T2640	9419-9424	NN	denotes	Abcg8
T2641	9425-9429	NN	denotes	gene
T2642	9430-9440	NN	denotes	disruption
T2643	9440-9441	.	denotes	.
T2644	9441-9583	sentence	denotes	Heterozygous offspring mice were back-crossed to C57BL/6J mice (n > 5) to produce disrupted line and inter-crossed to generate knockout mice.
T2645	9442-9454	JJ	denotes	Heterozygous
T2646	9465-9469	NNS	denotes	mice
T2647	9455-9464	NN	denotes	offspring
T2648	9480-9487	VBN	denotes	crossed
T2649	9470-9474	VBD	denotes	were
T2650	9475-9479	RB	denotes	back
T2651	9479-9480	HYPH	denotes	-
T2652	9488-9490	IN	denotes	to
T2653	9491-9496	NN	denotes	C57BL
T2654	9497-9499	NN	denotes	6J
T2655	9496-9497	HYPH	denotes	/
T2656	9500-9504	NNS	denotes	mice
T2657	9505-9506	-LRB-	denotes	(
T2658	9510-9511	CD	denotes	5
T2659	9506-9507	NN	denotes	n
T2660	9508-9509	SYM	denotes	>
T2661	9511-9512	-RRB-	denotes	)
T2662	9513-9515	TO	denotes	to
T2663	9516-9523	VB	denotes	produce
T2664	9524-9533	VBN	denotes	disrupted
T2665	9534-9538	NN	denotes	line
T2666	9539-9542	CC	denotes	and
T2667	9543-9556	VBN	denotes	inter-crossed
T2668	9557-9559	TO	denotes	to
T2669	9560-9568	VB	denotes	generate
T2670	9569-9577	NN	denotes	knockout
T2671	9578-9582	NNS	denotes	mice
T2672	9582-9583	.	denotes	.
T2715	9585-9592	NNS	denotes	Animals
T2716	9593-9596	CC	denotes	and
T2717	9597-9602	NNS	denotes	diets
T2718	9602-9863	sentence	denotes	All mice were maintained on a standard rodent chow (Harlan Teklad mouse/rat diet LM-485) which contained 61 mg/kg cholesterol, 31 mg/kg campesterol, and 105 mg/kg sitosterol, given free access to water and maintained at 25°C with a 12-h light, 12-h dark cycle.
T2719	9603-9606	DT	denotes	All
T2720	9607-9611	NNS	denotes	mice
T2721	9617-9627	VBN	denotes	maintained
T2722	9612-9616	VBD	denotes	were
T2723	9628-9630	IN	denotes	on
T2724	9631-9632	DT	denotes	a
T2725	9649-9653	NN	denotes	chow
T2726	9633-9641	JJ	denotes	standard
T2727	9642-9648	NN	denotes	rodent
T2728	9654-9655	-LRB-	denotes	(
T2729	9655-9661	NNP	denotes	Harlan
T2730	9662-9668	NNP	denotes	Teklad
T2731	9684-9686	NN	denotes	LM
T2732	9669-9674	NN	denotes	mouse
T2733	9675-9678	NN	denotes	rat
T2734	9674-9675	HYPH	denotes	/
T2735	9679-9683	NN	denotes	diet
T2736	9686-9687	HYPH	denotes	-
T2737	9687-9690	CD	denotes	485
T2738	9690-9691	-RRB-	denotes	)
T2739	9692-9697	WDT	denotes	which
T2740	9698-9707	VBD	denotes	contained
T2741	9708-9710	CD	denotes	61
T2742	9711-9713	NN	denotes	mg
T2743	9717-9728	NN	denotes	cholesterol
T2744	9713-9714	SYM	denotes	/
T2745	9714-9716	NN	denotes	kg
T2746	9728-9730	,	denotes	,
T2747	9730-9732	CD	denotes	31
T2748	9733-9735	NN	denotes	mg
T2749	9739-9750	NN	denotes	campesterol
T2750	9735-9736	SYM	denotes	/
T2751	9736-9738	NN	denotes	kg
T2752	9750-9752	,	denotes	,
T2753	9752-9755	CC	denotes	and
T2754	9756-9759	CD	denotes	105
T2755	9760-9762	NN	denotes	mg
T2756	9766-9776	NN	denotes	sitosterol
T2757	9762-9763	SYM	denotes	/
T2758	9763-9765	NN	denotes	kg
T2759	9776-9778	,	denotes	,
T2760	9778-9783	VBN	denotes	given
T2761	9784-9788	JJ	denotes	free
T2762	9789-9795	NN	denotes	access
T2763	9796-9798	IN	denotes	to
T2764	9799-9804	NN	denotes	water
T2765	9805-9808	CC	denotes	and
T2766	9809-9819	VBN	denotes	maintained
T2767	9820-9822	IN	denotes	at
T2768	9823-9825	CD	denotes	25
T2769	9825-9827	NN	denotes	°C
T2770	9828-9832	IN	denotes	with
T2771	9833-9834	DT	denotes	a
T2772	9857-9862	NN	denotes	cycle
T2773	9835-9837	CD	denotes	12
T2774	9838-9839	NN	denotes	h
T2775	9837-9838	HYPH	denotes	-
T2776	9840-9845	JJ	denotes	light
T2777	9845-9847	,	denotes	,
T2778	9847-9849	CD	denotes	12
T2779	9850-9851	NN	denotes	h
T2780	9849-9850	HYPH	denotes	-
T2781	9852-9856	JJ	denotes	dark
T2782	9862-9863	.	denotes	.
T2783	9863-9991	sentence	denotes	All animals were housed in the facilities for laboratory animals provided by the Division of Laboratory Animal Resources ACLAM.
T2784	9864-9867	DT	denotes	All
T2785	9868-9875	NNS	denotes	animals
T2786	9881-9887	VBN	denotes	housed
T2787	9876-9880	VBD	denotes	were
T2788	9888-9890	IN	denotes	in
T2789	9891-9894	DT	denotes	the
T2790	9895-9905	NNS	denotes	facilities
T2791	9906-9909	IN	denotes	for
T2792	9910-9920	NN	denotes	laboratory
T2793	9921-9928	NNS	denotes	animals
T2794	9929-9937	VBN	denotes	provided
T2795	9938-9940	IN	denotes	by
T2796	9941-9944	DT	denotes	the
T2797	9945-9953	NNP	denotes	Division
T2798	9954-9956	IN	denotes	of
T2799	9957-9967	NNP	denotes	Laboratory
T2800	9968-9974	NNP	denotes	Animal
T2801	9975-9984	NNPS	denotes	Resources
T2802	9985-9990	NNP	denotes	ACLAM
T2803	9990-9991	.	denotes	.
T2804	9991-10119	sentence	denotes	The Institutional Animal Care and Research Advisory Committee at Medical University of South Carolina approved all experiments.
T2805	9992-9995	DT	denotes	The
T2806	10044-10053	NNP	denotes	Committee
T2807	9996-10009	NNP	denotes	Institutional
T2808	10010-10016	NNP	denotes	Animal
T2809	10017-10021	NNP	denotes	Care
T2810	10022-10025	CC	denotes	and
T2811	10026-10034	NNP	denotes	Research
T2812	10035-10043	NNP	denotes	Advisory
T2813	10094-10102	VBD	denotes	approved
T2814	10054-10056	IN	denotes	at
T2815	10057-10064	NNP	denotes	Medical
T2816	10065-10075	NNP	denotes	University
T2817	10076-10078	IN	denotes	of
T2818	10079-10084	NNP	denotes	South
T2819	10085-10093	NNP	denotes	Carolina
T2820	10103-10106	DT	denotes	all
T2821	10107-10118	NNS	denotes	experiments
T2822	10118-10119	.	denotes	.
T2918	10121-10131	NN	denotes	Genotyping
T2919	10132-10134	IN	denotes	by
T2920	10135-10138	NN	denotes	PCR
T2921	10138-10376	sentence	denotes	Mouse tail DNA was isolated and purified by cutting approximately 0.5 cm of mouse tail and digesting it in 500 μl lysis buffer (50 mM Tris-HCl, pH8.0, 100 mM EDTA, 125 mM NaCl, 1% SDS, 200 μg Proteinase K) with rocking overnight at 55°C.
T2922	10139-10144	NN	denotes	Mouse
T2923	10145-10149	NN	denotes	tail
T2924	10150-10153	NN	denotes	DNA
T2925	10158-10166	VBN	denotes	isolated
T2926	10154-10157	VBD	denotes	was
T2927	10167-10170	CC	denotes	and
T2928	10171-10179	VBN	denotes	purified
T2929	10180-10182	IN	denotes	by
T2930	10183-10190	VBG	denotes	cutting
T2931	10191-10204	RB	denotes	approximately
T2932	10205-10208	CD	denotes	0.5
T2933	10209-10211	NN	denotes	cm
T2934	10212-10214	IN	denotes	of
T2935	10215-10220	NN	denotes	mouse
T2936	10221-10225	NN	denotes	tail
T2937	10226-10229	CC	denotes	and
T2938	10230-10239	VBG	denotes	digesting
T2939	10240-10242	PRP	denotes	it
T2940	10243-10245	IN	denotes	in
T2941	10246-10249	CD	denotes	500
T2942	10250-10252	NN	denotes	μl
T2943	10259-10265	NN	denotes	buffer
T2944	10253-10258	NN	denotes	lysis
T2945	10266-10267	-LRB-	denotes	(
T2946	10278-10281	NN	denotes	HCl
T2947	10267-10269	CD	denotes	50
T2948	10270-10272	NN	denotes	mM
T2949	10273-10277	NN	denotes	Tris
T2950	10277-10278	HYPH	denotes	-
T2951	10281-10283	,	denotes	,
T2952	10283-10285	NN	denotes	pH
T2953	10285-10288	CD	denotes	8.0
T2954	10288-10290	,	denotes	,
T2955	10290-10293	CD	denotes	100
T2956	10294-10296	NN	denotes	mM
T2957	10297-10301	NN	denotes	EDTA
T2958	10301-10303	,	denotes	,
T2959	10303-10306	CD	denotes	125
T2960	10307-10309	NN	denotes	mM
T2961	10310-10314	NN	denotes	NaCl
T2962	10314-10316	,	denotes	,
T2963	10316-10317	CD	denotes	1
T2964	10317-10318	NN	denotes	%
T2965	10319-10322	NN	denotes	SDS
T2966	10322-10324	,	denotes	,
T2967	10324-10327	CD	denotes	200
T2968	10328-10330	NN	denotes	μg
T2969	10342-10343	NN	denotes	K
T2970	10331-10341	NN	denotes	Proteinase
T2971	10343-10344	-RRB-	denotes	)
T2972	10345-10349	IN	denotes	with
T2973	10350-10357	VBG	denotes	rocking
T2974	10358-10367	RB	denotes	overnight
T2975	10368-10370	IN	denotes	at
T2976	10371-10373	CD	denotes	55
T2977	10373-10375	NN	denotes	°C
T2978	10375-10376	.	denotes	.
T2979	10376-10478	sentence	denotes	200 μl saturated NaCl was added into digested solution with vigorous shaking for at least 60 seconds.
T2980	10377-10380	CD	denotes	200
T2981	10381-10383	NN	denotes	μl
T2982	10394-10398	NN	denotes	NaCl
T2983	10384-10393	VBN	denotes	saturated
T2984	10403-10408	VBN	denotes	added
T2985	10399-10402	VBD	denotes	was
T2986	10409-10413	IN	denotes	into
T2987	10414-10422	JJ	denotes	digested
T2988	10423-10431	NN	denotes	solution
T2989	10432-10436	IN	denotes	with
T2990	10437-10445	JJ	denotes	vigorous
T2991	10446-10453	VBG	denotes	shaking
T2992	10454-10457	IN	denotes	for
T2993	10458-10460	RB	denotes	at
T2994	10467-10469	CD	denotes	60
T2995	10461-10466	RBS	denotes	least
T2996	10470-10477	NNS	denotes	seconds
T2997	10477-10478	.	denotes	.
T2998	10478-10560	sentence	denotes	Mixed solution was spun down at maximum speed in top micro-centrifuge for 20 min.
T2999	10479-10484	VBN	denotes	Mixed
T3000	10485-10493	NN	denotes	solution
T3001	10498-10502	VBN	denotes	spun
T3002	10494-10497	VBD	denotes	was
T3003	10503-10507	RP	denotes	down
T3004	10508-10510	IN	denotes	at
T3005	10511-10518	JJ	denotes	maximum
T3006	10519-10524	NN	denotes	speed
T3007	10525-10527	IN	denotes	in
T3008	10528-10531	JJ	denotes	top
T3009	10538-10548	NN	denotes	centrifuge
T3010	10532-10537	NN	denotes	micro
T3011	10537-10538	HYPH	denotes	-
T3012	10549-10552	IN	denotes	for
T3013	10553-10555	CD	denotes	20
T3014	10556-10559	NN	denotes	min
T3015	10559-10560	.	denotes	.
T3016	10560-10658	sentence	denotes	The supernatant was transferred to a new tube with equal volume 100% EtOH and mixed by inversion.
T3017	10561-10564	DT	denotes	The
T3018	10565-10576	NN	denotes	supernatant
T3019	10581-10592	VBN	denotes	transferred
T3020	10577-10580	VBD	denotes	was
T3021	10593-10595	IN	denotes	to
T3022	10596-10597	DT	denotes	a
T3023	10602-10606	NN	denotes	tube
T3024	10598-10601	JJ	denotes	new
T3025	10607-10611	IN	denotes	with
T3026	10612-10617	JJ	denotes	equal
T3027	10630-10634	NN	denotes	EtOH
T3028	10618-10624	NN	denotes	volume
T3029	10625-10628	CD	denotes	100
T3030	10628-10629	NN	denotes	%
T3031	10635-10638	CC	denotes	and
T3032	10639-10644	VBN	denotes	mixed
T3033	10645-10647	IN	denotes	by
T3034	10648-10657	NN	denotes	inversion
T3035	10657-10658	.	denotes	.
T3036	10658-10737	sentence	denotes	The DNA pellet was washed in 70% EtOH and resuspended in 100–150 μl TE buffer.
T3037	10659-10662	DT	denotes	The
T3038	10667-10673	NN	denotes	pellet
T3039	10663-10666	NN	denotes	DNA
T3040	10678-10684	VBN	denotes	washed
T3041	10674-10677	VBD	denotes	was
T3042	10685-10687	IN	denotes	in
T3043	10688-10690	CD	denotes	70
T3044	10690-10691	NN	denotes	%
T3045	10692-10696	NN	denotes	EtOH
T3046	10697-10700	CC	denotes	and
T3047	10701-10712	VBD	denotes	resuspended
T3048	10713-10715	IN	denotes	in
T3049	10716-10719	CD	denotes	100
T3050	10720-10723	CD	denotes	150
T3051	10719-10720	SYM	denotes	–
T3052	10724-10726	NN	denotes	μl
T3053	10730-10736	NN	denotes	buffer
T3054	10727-10729	NN	denotes	TE
T3055	10736-10737	.	denotes	.
T3056	10737-10844	sentence	denotes	UV spectrophotometry and electrophoresis were used to analyze the quality and quantity of the genomic DNA.
T3057	10738-10740	NN	denotes	UV
T3058	10741-10758	NN	denotes	spectrophotometry
T3059	10784-10788	VBN	denotes	used
T3060	10759-10762	CC	denotes	and
T3061	10763-10778	NN	denotes	electrophoresis
T3062	10779-10783	VBD	denotes	were
T3063	10789-10791	TO	denotes	to
T3064	10792-10799	VB	denotes	analyze
T3065	10800-10803	DT	denotes	the
T3066	10804-10811	NN	denotes	quality
T3067	10812-10815	CC	denotes	and
T3068	10816-10824	NN	denotes	quantity
T3069	10825-10827	IN	denotes	of
T3070	10828-10831	DT	denotes	the
T3071	10840-10843	NN	denotes	DNA
T3072	10832-10839	JJ	denotes	genomic
T3073	10843-10844	.	denotes	.
T3074	10844-11082	sentence	denotes	Genotyping was performed by multiplex PCR reaction using three separate primers (two Abcg8 gene specific primers, mg8-in3F 5'-CCCAATGATGAATGAGACGCT-3', mg8-R9 5'-TTTGTCACGCTGGGCCTGG-3' and neoF for identification of Abcg8 target status).
T3075	10845-10855	NN	denotes	Genotyping
T3076	10860-10869	VBN	denotes	performed
T3077	10856-10859	VBD	denotes	was
T3078	10870-10872	IN	denotes	by
T3079	10873-10882	JJ	denotes	multiplex
T3080	10887-10895	NN	denotes	reaction
T3081	10883-10886	NN	denotes	PCR
T3082	10896-10901	VBG	denotes	using
T3083	10902-10907	CD	denotes	three
T3084	10917-10924	NNS	denotes	primers
T3085	10908-10916	JJ	denotes	separate
T3086	10925-10926	-LRB-	denotes	(
T3087	10926-10929	CD	denotes	two
T3088	10950-10957	NNS	denotes	primers
T3089	10930-10935	NN	denotes	Abcg8
T3090	10936-10940	NN	denotes	gene
T3091	10941-10949	JJ	denotes	specific
T3092	10957-10959	,	denotes	,
T3093	10959-10962	NN	denotes	mg8
T3094	10963-10967	NN	denotes	in3F
T3095	10962-10963	HYPH	denotes	-
T3096	10971-10992	NN	denotes	CCCAATGATGAATGAGACGCT
T3097	10968-10969	CD	denotes	5
T3098	10969-10970	SYM	denotes	'
T3099	10970-10971	HYPH	denotes	-
T3100	10992-10993	HYPH	denotes	-
T3101	10993-10994	CD	denotes	3
T3102	10994-10995	SYM	denotes	'
T3103	10995-10997	,	denotes	,
T3104	10997-11000	NN	denotes	mg8
T3105	11001-11003	NN	denotes	R9
T3106	11000-11001	HYPH	denotes	-
T3107	11007-11026	NN	denotes	TTTGTCACGCTGGGCCTGG
T3108	11004-11005	CD	denotes	5
T3109	11005-11006	SYM	denotes	'
T3110	11006-11007	HYPH	denotes	-
T3111	11026-11027	HYPH	denotes	-
T3112	11027-11028	CD	denotes	3
T3113	11028-11029	SYM	denotes	'
T3114	11030-11033	CC	denotes	and
T3115	11034-11038	NN	denotes	neoF
T3116	11039-11042	IN	denotes	for
T3117	11043-11057	NN	denotes	identification
T3118	11058-11060	IN	denotes	of
T3119	11061-11066	NN	denotes	Abcg8
T3120	11074-11080	NN	denotes	status
T3121	11067-11073	NN	denotes	target
T3122	11080-11081	-RRB-	denotes	)
T3123	11081-11082	.	denotes	.
T3124	11082-11256	sentence	denotes	The PCR reaction consisted of 1x PCR buffer (1.5 mM MgCl2, 16 mM (NH4)2SO4, 0.1 mM dNTPs, 67 mM Tris-HCl (pH8.8)) 1 μM of each primer, water and 1.0 units of Taq polymerase.
T3125	11083-11086	DT	denotes	The
T3126	11091-11099	NN	denotes	reaction
T3127	11087-11090	NN	denotes	PCR
T3128	11100-11109	VBD	denotes	consisted
T3129	11110-11112	IN	denotes	of
T3130	11113-11115	NN	denotes	1x
T3131	11120-11126	NN	denotes	buffer
T3132	11116-11119	NN	denotes	PCR
T3133	11199-11201	NN	denotes	μM
T3134	11127-11128	-LRB-	denotes	(
T3135	11135-11140	NN	denotes	MgCl2
T3136	11128-11131	CD	denotes	1.5
T3137	11132-11134	NN	denotes	mM
T3138	11140-11142	,	denotes	,
T3139	11142-11144	CD	denotes	16
T3140	11145-11147	NN	denotes	mM
T3141	11153-11157	NN	denotes	2SO4
T3142	11148-11149	-LRB-	denotes	(
T3143	11149-11152	NN	denotes	NH4
T3144	11152-11153	-RRB-	denotes	)
T3145	11157-11159	,	denotes	,
T3146	11159-11162	CD	denotes	0.1
T3147	11163-11165	NN	denotes	mM
T3148	11166-11171	NNS	denotes	dNTPs
T3149	11171-11173	,	denotes	,
T3150	11173-11175	CD	denotes	67
T3151	11176-11178	NN	denotes	mM
T3152	11184-11187	NN	denotes	HCl
T3153	11179-11183	NN	denotes	Tris
T3154	11183-11184	HYPH	denotes	-
T3155	11188-11189	-LRB-	denotes	(
T3156	11189-11191	NN	denotes	pH
T3157	11191-11194	CD	denotes	8.8
T3158	11194-11195	-RRB-	denotes	)
T3159	11195-11196	-RRB-	denotes	)
T3160	11197-11198	CD	denotes	1
T3161	11202-11204	IN	denotes	of
T3162	11205-11209	DT	denotes	each
T3163	11210-11216	NN	denotes	primer
T3164	11216-11218	,	denotes	,
T3165	11218-11223	NN	denotes	water
T3166	11224-11227	CC	denotes	and
T3167	11228-11231	CD	denotes	1.0
T3168	11232-11237	NNS	denotes	units
T3169	11238-11240	IN	denotes	of
T3170	11241-11244	NN	denotes	Taq
T3171	11245-11255	NN	denotes	polymerase
T3172	11255-11256	.	denotes	.
T3173	11256-11358	sentence	denotes	The reactions were cycled as follows: 94°C for 30 sec, 60°C for 30 sec, 72°C for 1 min for 35 cycles.
T3174	11257-11260	DT	denotes	The
T3175	11261-11270	NNS	denotes	reactions
T3176	11276-11282	VBN	denotes	cycled
T3177	11271-11275	VBD	denotes	were
T3178	11283-11285	IN	denotes	as
T3179	11286-11293	VBZ	denotes	follows
T3180	11293-11295	:	denotes	:
T3181	11295-11297	CD	denotes	94
T3182	11297-11299	NN	denotes	°C
T3183	11300-11303	IN	denotes	for
T3184	11304-11306	CD	denotes	30
T3185	11307-11310	NN	denotes	sec
T3186	11310-11312	,	denotes	,
T3187	11312-11314	CD	denotes	60
T3188	11314-11316	NN	denotes	°C
T3189	11317-11320	IN	denotes	for
T3190	11321-11323	CD	denotes	30
T3191	11324-11327	NN	denotes	sec
T3192	11327-11329	,	denotes	,
T3193	11329-11331	CD	denotes	72
T3194	11331-11333	NN	denotes	°C
T3195	11334-11337	IN	denotes	for
T3196	11338-11339	CD	denotes	1
T3197	11340-11343	NN	denotes	min
T3198	11344-11347	IN	denotes	for
T3199	11348-11350	CD	denotes	35
T3200	11351-11357	NNS	denotes	cycles
T3201	11357-11358	.	denotes	.
T3202	11358-11411	sentence	denotes	The PCR products were identified by electrophoresis.
T3203	11359-11362	DT	denotes	The
T3204	11367-11375	NNS	denotes	products
T3205	11363-11366	NN	denotes	PCR
T3206	11381-11391	VBN	denotes	identified
T3207	11376-11380	VBD	denotes	were
T3208	11392-11394	IN	denotes	by
T3209	11395-11410	NN	denotes	electrophoresis
T3210	11410-11411	.	denotes	.
T3239	11413-11421	NNP	denotes	Southern
T3240	11422-11426	NN	denotes	blot
T3241	11427-11435	NN	denotes	analysis
T3242	11435-11577	sentence	denotes	To confirm PCR results, DNAs were digested with Bam HI, subjected to electrophoresis in a 0.6% agarose gel and transferred to nylon membrane.
T3243	11436-11438	TO	denotes	To
T3244	11439-11446	VB	denotes	confirm
T3245	11470-11478	VBN	denotes	digested
T3246	11447-11450	NN	denotes	PCR
T3247	11451-11458	NNS	denotes	results
T3248	11458-11460	,	denotes	,
T3249	11460-11464	NNS	denotes	DNAs
T3250	11465-11469	VBD	denotes	were
T3251	11479-11483	IN	denotes	with
T3252	11484-11487	NN	denotes	Bam
T3253	11488-11490	NN	denotes	HI
T3254	11490-11492	,	denotes	,
T3255	11492-11501	VBN	denotes	subjected
T3256	11502-11504	IN	denotes	to
T3257	11505-11520	NN	denotes	electrophoresis
T3258	11521-11523	IN	denotes	in
T3259	11524-11525	DT	denotes	a
T3260	11539-11542	NN	denotes	gel
T3261	11526-11529	CD	denotes	0.6
T3262	11529-11530	NN	denotes	%
T3263	11531-11538	NN	denotes	agarose
T3264	11543-11546	CC	denotes	and
T3265	11547-11558	VBN	denotes	transferred
T3266	11559-11561	IN	denotes	to
T3267	11562-11567	NN	denotes	nylon
T3268	11568-11576	NN	denotes	membrane
T3269	11576-11577	.	denotes	.
T3270	11577-11741	sentence	denotes	Southern blot filters were hybridized with a 562 bp PCR fragment from partial intron 2 and exon 3 of Abcg8 32P-randomly-labeled probe as previously described [22].
T3271	11578-11586	NNP	denotes	Southern
T3272	11587-11591	NN	denotes	blot
T3273	11592-11599	NNS	denotes	filters
T3274	11605-11615	VBN	denotes	hybridized
T3275	11600-11604	VBD	denotes	were
T3276	11616-11620	IN	denotes	with
T3277	11621-11622	DT	denotes	a
T3278	11634-11642	NN	denotes	fragment
T3279	11623-11626	CD	denotes	562
T3280	11627-11629	NN	denotes	bp
T3281	11630-11633	NN	denotes	PCR
T3282	11643-11647	IN	denotes	from
T3283	11648-11655	JJ	denotes	partial
T3284	11656-11662	NN	denotes	intron
T3285	11663-11664	CD	denotes	2
T3286	11665-11668	CC	denotes	and
T3287	11669-11673	NN	denotes	exon
T3288	11674-11675	CD	denotes	3
T3289	11676-11678	IN	denotes	of
T3290	11679-11684	NN	denotes	Abcg8
T3291	11706-11711	NN	denotes	probe
T3292	11685-11688	NN	denotes	32P
T3293	11698-11705	VBN	denotes	labeled
T3294	11688-11689	HYPH	denotes	-
T3295	11689-11697	RB	denotes	randomly
T3296	11697-11698	HYPH	denotes	-
T3297	11712-11714	IN	denotes	as
T3298	11726-11735	VBN	denotes	described
T3299	11715-11725	RB	denotes	previously
T3300	11736-11737	-LRB-	denotes	[
T3301	11737-11739	CD	denotes	22
T3302	11739-11740	-RRB-	denotes	]
T3303	11740-11741	.	denotes	.
T3344	11743-11751	NNP	denotes	Northern
T3345	11752-11756	NN	denotes	blot
T3346	11757-11765	NN	denotes	analysis
T3347	11765-11923	sentence	denotes	Isolation of liver and intestinal total RNA from Abcg8+/+, Abcg8+/- and Abcg8-/- mice and Northern blot analyses were performed as previously described [23].
T3348	11766-11775	NN	denotes	Isolation
T3349	11884-11893	VBN	denotes	performed
T3350	11776-11778	IN	denotes	of
T3351	11779-11784	NN	denotes	liver
T3352	11789-11799	JJ	denotes	intestinal
T3353	11785-11788	CC	denotes	and
T3354	11806-11809	NN	denotes	RNA
T3355	11800-11805	JJ	denotes	total
T3356	11810-11814	IN	denotes	from
T3357	11815-11820	NN	denotes	Abcg8
T3358	11847-11851	NNS	denotes	mice
T3359	11820-11821	SYM	denotes	+
T3360	11821-11822	HYPH	denotes	/
T3361	11822-11823	SYM	denotes	+
T3362	11823-11825	,	denotes	,
T3363	11825-11830	NN	denotes	Abcg8
T3364	11830-11831	SYM	denotes	+
T3365	11831-11832	HYPH	denotes	/
T3366	11832-11833	SYM	denotes	-
T3367	11834-11837	CC	denotes	and
T3368	11838-11843	NN	denotes	Abcg8
T3369	11843-11844	SYM	denotes	-
T3370	11844-11845	HYPH	denotes	/
T3371	11845-11846	SYM	denotes	-
T3372	11852-11855	CC	denotes	and
T3373	11856-11864	NNP	denotes	Northern
T3374	11865-11869	NN	denotes	blot
T3375	11870-11878	NNS	denotes	analyses
T3376	11879-11883	VBD	denotes	were
T3377	11894-11896	IN	denotes	as
T3378	11908-11917	VBN	denotes	described
T3379	11897-11907	RB	denotes	previously
T3380	11918-11919	-LRB-	denotes	[
T3381	11919-11921	CD	denotes	23
T3382	11921-11922	-RRB-	denotes	]
T3383	11922-11923	.	denotes	.
T3384	11923-12088	sentence	denotes	The probe for Abcg5 was 1964 bp (nt 136–2099, GeneBank™ accession no. AH011511) and the probe for Abcg8 was 2019 bp (nt 103–2126, GeneBank™ accession no. AH011518).
T3385	11924-11927	DT	denotes	The
T3386	11928-11933	NN	denotes	probe
T3387	11944-11947	VBD	denotes	was
T3388	11934-11937	IN	denotes	for
T3389	11938-11943	NN	denotes	Abcg5
T3390	11948-11952	CD	denotes	1964
T3391	11953-11955	NN	denotes	bp
T3392	11956-11957	-LRB-	denotes	(
T3393	11994-12002	NN	denotes	AH011511
T3394	11957-11959	NN	denotes	nt
T3395	11960-11963	CD	denotes	136
T3396	11963-11964	SYM	denotes	–
T3397	11964-11968	CD	denotes	2099
T3398	11968-11970	,	denotes	,
T3399	11970-11979	NNP	denotes	GeneBank™
T3400	11980-11989	NN	denotes	accession
T3401	11990-11993	NN	denotes	no.
T3402	12002-12003	-RRB-	denotes	)
T3403	12004-12007	CC	denotes	and
T3404	12008-12011	DT	denotes	the
T3405	12012-12017	NN	denotes	probe
T3406	12028-12031	VBD	denotes	was
T3407	12018-12021	IN	denotes	for
T3408	12022-12027	NN	denotes	Abcg8
T3409	12032-12036	CD	denotes	2019
T3410	12037-12039	NN	denotes	bp
T3411	12040-12041	-LRB-	denotes	(
T3412	12078-12086	NN	denotes	AH011518
T3413	12041-12043	NN	denotes	nt
T3414	12044-12047	CD	denotes	103
T3415	12047-12048	SYM	denotes	–
T3416	12048-12052	CD	denotes	2126
T3417	12052-12054	,	denotes	,
T3418	12054-12063	NNP	denotes	GeneBank™
T3419	12064-12073	NN	denotes	accession
T3420	12074-12077	NN	denotes	no.
T3421	12086-12087	-RRB-	denotes	)
T3422	12087-12088	.	denotes	.
T3461	12090-12097	JJ	denotes	Reverse
T3462	12098-12111	NN	denotes	Transcription
T3463	12117-12120	NN	denotes	PCR
T3464	12112-12113	-LRB-	denotes	(
T3465	12113-12115	NN	denotes	RT
T3466	12115-12116	-RRB-	denotes	)
T3467	12116-12117	HYPH	denotes	-
T3468	12120-12248	sentence	denotes	To confirm that there was no alternative splicing of the disrupted allele, RT-PCR was performed on cDNAs of pooled mouse liver.
T3469	12121-12123	TO	denotes	To
T3470	12124-12131	VB	denotes	confirm
T3471	12207-12216	VBN	denotes	performed
T3472	12132-12136	IN	denotes	that
T3473	12143-12146	VBD	denotes	was
T3474	12137-12142	EX	denotes	there
T3475	12147-12149	DT	denotes	no
T3476	12162-12170	NN	denotes	splicing
T3477	12150-12161	JJ	denotes	alternative
T3478	12171-12173	IN	denotes	of
T3479	12174-12177	DT	denotes	the
T3480	12188-12194	NN	denotes	allele
T3481	12178-12187	VBN	denotes	disrupted
T3482	12194-12196	,	denotes	,
T3483	12196-12198	NN	denotes	RT
T3484	12199-12202	NN	denotes	PCR
T3485	12198-12199	HYPH	denotes	-
T3486	12203-12206	VBD	denotes	was
T3487	12217-12219	IN	denotes	on
T3488	12220-12225	NNS	denotes	cDNAs
T3489	12226-12228	IN	denotes	of
T3490	12229-12235	VBN	denotes	pooled
T3491	12242-12247	NN	denotes	liver
T3492	12236-12241	NN	denotes	mouse
T3493	12247-12248	.	denotes	.
T3494	12248-12502	sentence	denotes	Briefly, samples of total RNA (0.5 μg) from pooled mouse livers (n = 4) were reverse transcribed according to the SuperScript™ First-Strand Synthesis System (Invitrogen, Carlsbad, CA, USA) using random hexamers in a final total reaction volume of 20 μl.
T3495	12249-12256	RB	denotes	Briefly
T3496	12334-12345	VBN	denotes	transcribed
T3497	12256-12258	,	denotes	,
T3498	12258-12265	NNS	denotes	samples
T3499	12266-12268	IN	denotes	of
T3500	12269-12274	JJ	denotes	total
T3501	12275-12278	NN	denotes	RNA
T3502	12279-12280	-LRB-	denotes	(
T3503	12284-12286	NN	denotes	μg
T3504	12280-12283	CD	denotes	0.5
T3505	12286-12287	-RRB-	denotes	)
T3506	12288-12292	IN	denotes	from
T3507	12293-12299	VBN	denotes	pooled
T3508	12306-12312	NNS	denotes	livers
T3509	12300-12305	NN	denotes	mouse
T3510	12313-12314	-LRB-	denotes	(
T3511	12318-12319	CD	denotes	4
T3512	12314-12315	NN	denotes	n
T3513	12316-12317	SYM	denotes	=
T3514	12319-12320	-RRB-	denotes	)
T3515	12321-12325	VBD	denotes	were
T3516	12326-12333	RB	denotes	reverse
T3517	12346-12355	VBG	denotes	according
T3518	12356-12358	IN	denotes	to
T3519	12359-12362	DT	denotes	the
T3520	12399-12405	NN	denotes	System
T3521	12363-12375	NNP	denotes	SuperScript™
T3522	12376-12381	JJ	denotes	First
T3523	12382-12388	NN	denotes	Strand
T3524	12381-12382	HYPH	denotes	-
T3525	12389-12398	NN	denotes	Synthesis
T3526	12406-12407	-LRB-	denotes	(
T3527	12407-12417	NNP	denotes	Invitrogen
T3528	12417-12419	,	denotes	,
T3529	12419-12427	NNP	denotes	Carlsbad
T3530	12427-12429	,	denotes	,
T3531	12429-12431	NNP	denotes	CA
T3532	12431-12433	,	denotes	,
T3533	12433-12436	NNP	denotes	USA
T3534	12436-12437	-RRB-	denotes	)
T3535	12438-12443	VBG	denotes	using
T3536	12444-12450	JJ	denotes	random
T3537	12451-12459	NNS	denotes	hexamers
T3538	12460-12462	IN	denotes	in
T3539	12463-12464	DT	denotes	a
T3540	12486-12492	NN	denotes	volume
T3541	12465-12470	JJ	denotes	final
T3542	12471-12476	JJ	denotes	total
T3543	12477-12485	NN	denotes	reaction
T3544	12493-12495	IN	denotes	of
T3545	12496-12498	CD	denotes	20
T3546	12499-12501	NN	denotes	μl
T3547	12501-12502	.	denotes	.
T3548	12502-12803	sentence	denotes	RT-PCR was performed using primers located outside of the targeted region: F1 (CCTCAGCTGGTGAGGAGGTG) with R1 (GATGGAGAAGGTGAAGTTGCC), F2 (ATTTCCAATGACTTCCGGGAC) with R1 and F3 (CTGGAAGACGGGCTGTACACT) with R1, to produce expected product lengths of 1449, 654 and 429 bp respectively in wild-type cDNA.
T3549	12503-12505	NN	denotes	RT
T3550	12506-12509	NN	denotes	PCR
T3551	12505-12506	HYPH	denotes	-
T3552	12514-12523	VBN	denotes	performed
T3553	12510-12513	VBD	denotes	was
T3554	12524-12529	VBG	denotes	using
T3555	12530-12537	NNS	denotes	primers
T3556	12538-12545	VBN	denotes	located
T3557	12546-12553	IN	denotes	outside
T3558	12554-12556	IN	denotes	of
T3559	12557-12560	DT	denotes	the
T3560	12570-12576	NN	denotes	region
T3561	12561-12569	VBN	denotes	targeted
T3562	12576-12578	:	denotes	:
T3563	12578-12580	NN	denotes	F1
T3564	12581-12582	-LRB-	denotes	(
T3565	12582-12602	NN	denotes	CCTCAGCTGGTGAGGAGGTG
T3566	12602-12603	-RRB-	denotes	)
T3567	12604-12608	IN	denotes	with
T3568	12609-12611	NN	denotes	R1
T3569	12612-12613	-LRB-	denotes	(
T3570	12613-12634	NN	denotes	GATGGAGAAGGTGAAGTTGCC
T3571	12634-12635	-RRB-	denotes	)
T3572	12635-12637	,	denotes	,
T3573	12637-12639	NN	denotes	F2
T3574	12640-12641	-LRB-	denotes	(
T3575	12641-12662	NN	denotes	ATTTCCAATGACTTCCGGGAC
T3576	12662-12663	-RRB-	denotes	)
T3577	12664-12668	IN	denotes	with
T3578	12669-12671	NN	denotes	R1
T3579	12672-12675	CC	denotes	and
T3580	12676-12678	NN	denotes	F3
T3581	12679-12680	-LRB-	denotes	(
T3582	12680-12701	NN	denotes	CTGGAAGACGGGCTGTACACT
T3583	12701-12702	-RRB-	denotes	)
T3584	12703-12707	IN	denotes	with
T3585	12708-12710	NN	denotes	R1
T3586	12710-12712	,	denotes	,
T3587	12712-12714	TO	denotes	to
T3588	12715-12722	VB	denotes	produce
T3589	12723-12731	VBN	denotes	expected
T3590	12740-12747	NNS	denotes	lengths
T3591	12732-12739	NN	denotes	product
T3592	12748-12750	IN	denotes	of
T3593	12751-12755	CD	denotes	1449
T3594	12769-12771	NN	denotes	bp
T3595	12755-12757	,	denotes	,
T3596	12757-12760	CD	denotes	654
T3597	12761-12764	CC	denotes	and
T3598	12765-12768	CD	denotes	429
T3599	12772-12784	RB	denotes	respectively
T3600	12785-12787	IN	denotes	in
T3601	12788-12792	JJ	denotes	wild
T3602	12793-12797	NN	denotes	type
T3603	12792-12793	HYPH	denotes	-
T3604	12798-12802	NN	denotes	cDNA
T3605	12802-12803	.	denotes	.
T3689	12805-12817	JJ	denotes	Quantitative
T3690	12821-12824	NN	denotes	PCR
T3691	12818-12820	NN	denotes	RT
T3692	12820-12821	HYPH	denotes	-
T3693	12824-13066	sentence	denotes	Primers were based upon previously published primer sets [24-26] or designed using MacVector, which were designed from either mRNA or cDNA to avoid the amplification of potentially contaminating genomic DNA in the total RNA sample (Table 1).
T3694	12825-12832	NNS	denotes	Primers
T3695	12838-12843	VBN	denotes	based
T3696	12833-12837	VBD	denotes	were
T3697	12844-12848	IN	denotes	upon
T3698	12849-12859	RB	denotes	previously
T3699	12860-12869	VBN	denotes	published
T3700	12877-12881	NNS	denotes	sets
T3701	12870-12876	NN	denotes	primer
T3702	12882-12883	-LRB-	denotes	[
T3703	12883-12885	CD	denotes	24
T3704	12885-12886	SYM	denotes	-
T3705	12886-12888	CD	denotes	26
T3706	12888-12889	-RRB-	denotes	]
T3707	12890-12892	CC	denotes	or
T3708	12893-12901	VBN	denotes	designed
T3709	12902-12907	VBG	denotes	using
T3710	12908-12917	NNP	denotes	MacVector
T3711	12917-12919	,	denotes	,
T3712	12919-12924	WDT	denotes	which
T3713	12930-12938	VBN	denotes	designed
T3714	12925-12929	VBD	denotes	were
T3715	12939-12943	IN	denotes	from
T3716	12944-12950	CC	denotes	either
T3717	12951-12955	NN	denotes	mRNA
T3718	12956-12958	CC	denotes	or
T3719	12959-12963	NN	denotes	cDNA
T3720	12964-12966	TO	denotes	to
T3721	12967-12972	VB	denotes	avoid
T3722	12973-12976	DT	denotes	the
T3723	12977-12990	NN	denotes	amplification
T3724	12991-12993	IN	denotes	of
T3725	12994-13005	RB	denotes	potentially
T3726	13006-13019	VBG	denotes	contaminating
T3727	13028-13031	NN	denotes	DNA
T3728	13020-13027	JJ	denotes	genomic
T3729	13032-13034	IN	denotes	in
T3730	13035-13038	DT	denotes	the
T3731	13049-13055	NN	denotes	sample
T3732	13039-13044	JJ	denotes	total
T3733	13045-13048	NN	denotes	RNA
T3734	13056-13057	-LRB-	denotes	(
T3735	13057-13062	NN	denotes	Table
T3736	13063-13064	CD	denotes	1
T3737	13064-13065	-RRB-	denotes	)
T3738	13065-13066	.	denotes	.
T3739	13066-13313	sentence	denotes	Samples of total RNA (0.5 μg) from pooled mouse livers (n = 4) were reverse transcribed according to the SuperScript™ First-Strand Synthesis System (Invitrogen, Carlsbad, CA, USA) using random hexamers with a final total reaction volume of 20 μl.
T3740	13067-13074	NNS	denotes	Samples
T3741	13143-13154	VBN	denotes	transcribed
T3742	13075-13077	IN	denotes	of
T3743	13078-13083	JJ	denotes	total
T3744	13084-13087	NN	denotes	RNA
T3745	13088-13089	-LRB-	denotes	(
T3746	13093-13095	NN	denotes	μg
T3747	13089-13092	CD	denotes	0.5
T3748	13095-13096	-RRB-	denotes	)
T3749	13097-13101	IN	denotes	from
T3750	13102-13108	VBN	denotes	pooled
T3751	13115-13121	NNS	denotes	livers
T3752	13109-13114	NN	denotes	mouse
T3753	13122-13123	-LRB-	denotes	(
T3754	13127-13128	CD	denotes	4
T3755	13123-13124	NN	denotes	n
T3756	13125-13126	SYM	denotes	=
T3757	13128-13129	-RRB-	denotes	)
T3758	13130-13134	VBD	denotes	were
T3759	13135-13142	RB	denotes	reverse
T3760	13155-13164	VBG	denotes	according
T3761	13165-13167	IN	denotes	to
T3762	13168-13171	DT	denotes	the
T3763	13208-13214	NN	denotes	System
T3764	13172-13184	NNP	denotes	SuperScript™
T3765	13185-13190	JJ	denotes	First
T3766	13191-13197	NN	denotes	Strand
T3767	13190-13191	HYPH	denotes	-
T3768	13198-13207	NN	denotes	Synthesis
T3769	13215-13216	-LRB-	denotes	(
T3770	13216-13226	NNP	denotes	Invitrogen
T3771	13226-13228	,	denotes	,
T3772	13228-13236	NNP	denotes	Carlsbad
T3773	13236-13238	,	denotes	,
T3774	13238-13240	NNP	denotes	CA
T3775	13240-13242	,	denotes	,
T3776	13242-13245	NNP	denotes	USA
T3777	13245-13246	-RRB-	denotes	)
T3778	13247-13252	VBG	denotes	using
T3779	13253-13259	JJ	denotes	random
T3780	13260-13268	NNS	denotes	hexamers
T3781	13269-13273	IN	denotes	with
T3782	13274-13275	DT	denotes	a
T3783	13297-13303	NN	denotes	volume
T3784	13276-13281	JJ	denotes	final
T3785	13282-13287	JJ	denotes	total
T3786	13288-13296	NN	denotes	reaction
T3787	13304-13306	IN	denotes	of
T3788	13307-13309	CD	denotes	20
T3789	13310-13312	NN	denotes	μl
T3790	13312-13313	.	denotes	.
T3791	13313-13430	sentence	denotes	Quantitative RT-PCR was performed on a PE Biosystems GeneAmp® 5700 sequence Detection System (Forest City, CA, USA).
T3792	13314-13326	JJ	denotes	Quantitative
T3793	13330-13333	NN	denotes	PCR
T3794	13327-13329	NN	denotes	RT
T3795	13329-13330	HYPH	denotes	-
T3796	13338-13347	VBN	denotes	performed
T3797	13334-13337	VBD	denotes	was
T3798	13348-13350	IN	denotes	on
T3799	13351-13352	DT	denotes	a
T3800	13400-13406	NN	denotes	System
T3801	13353-13355	NN	denotes	PE
T3802	13356-13366	NNP	denotes	Biosystems
T3803	13367-13375	NNP	denotes	GeneAmp®
T3804	13376-13380	CD	denotes	5700
T3805	13381-13389	NN	denotes	sequence
T3806	13390-13399	NN	denotes	Detection
T3807	13407-13408	-LRB-	denotes	(
T3808	13415-13419	NNP	denotes	City
T3809	13408-13414	NNP	denotes	Forest
T3810	13419-13421	,	denotes	,
T3811	13421-13423	NNP	denotes	CA
T3812	13423-13425	,	denotes	,
T3813	13425-13428	NNP	denotes	USA
T3814	13428-13429	-RRB-	denotes	)
T3815	13429-13430	.	denotes	.
T3816	13430-13725	sentence	denotes	Standard reaction volume was 10 μl containing 1 × QuantiTect SYBR Green PCR master mix (Qiagen, Valencia, CA, USA), 0.002 U AmpErase UNG enzyme (PE Biosystems, Forest City, CA, USA), 0.7 μl of cDNA template from reverse transcription reaction as above and 100–500 nM of oligonucleotide primers.
T3817	13431-13439	JJ	denotes	Standard
T3818	13449-13455	NN	denotes	volume
T3819	13440-13448	NN	denotes	reaction
T3820	13456-13459	VBD	denotes	was
T3821	13460-13462	CD	denotes	10
T3822	13463-13465	NN	denotes	μl
T3823	13466-13476	VBG	denotes	containing
T3824	13477-13478	CD	denotes	1
T3825	13514-13517	NN	denotes	mix
T3826	13479-13480	SYM	denotes	×
T3827	13481-13491	NNP	denotes	QuantiTect
T3828	13492-13496	NNP	denotes	SYBR
T3829	13497-13502	NNP	denotes	Green
T3830	13503-13506	NN	denotes	PCR
T3831	13507-13513	NN	denotes	master
T3832	13518-13519	-LRB-	denotes	(
T3833	13519-13525	NNP	denotes	Qiagen
T3834	13525-13527	,	denotes	,
T3835	13527-13535	NNP	denotes	Valencia
T3836	13535-13537	,	denotes	,
T3837	13537-13539	NNP	denotes	CA
T3838	13539-13541	,	denotes	,
T3839	13541-13544	NNP	denotes	USA
T3840	13544-13545	-RRB-	denotes	)
T3841	13545-13547	,	denotes	,
T3842	13547-13552	CD	denotes	0.002
T3843	13553-13554	NN	denotes	U
T3844	13568-13574	NN	denotes	enzyme
T3845	13555-13563	NN	denotes	AmpErase
T3846	13564-13567	NN	denotes	UNG
T3847	13575-13576	-LRB-	denotes	(
T3848	13579-13589	NNP	denotes	Biosystems
T3849	13576-13578	NN	denotes	PE
T3850	13589-13591	,	denotes	,
T3851	13591-13597	NNP	denotes	Forest
T3852	13598-13602	NNP	denotes	City
T3853	13602-13604	,	denotes	,
T3854	13604-13606	NNP	denotes	CA
T3855	13606-13608	,	denotes	,
T3856	13608-13611	NNP	denotes	USA
T3857	13611-13612	-RRB-	denotes	)
T3858	13612-13614	,	denotes	,
T3859	13614-13617	CD	denotes	0.7
T3860	13618-13620	NN	denotes	μl
T3861	13621-13623	IN	denotes	of
T3862	13624-13628	NN	denotes	cDNA
T3863	13629-13637	NN	denotes	template
T3864	13638-13642	IN	denotes	from
T3865	13643-13650	JJ	denotes	reverse
T3866	13651-13664	NN	denotes	transcription
T3867	13665-13673	NN	denotes	reaction
T3868	13674-13676	IN	denotes	as
T3869	13677-13682	RB	denotes	above
T3870	13683-13686	CC	denotes	and
T3871	13687-13690	CD	denotes	100
T3872	13691-13694	CD	denotes	500
T3873	13690-13691	SYM	denotes	–
T3874	13695-13697	NN	denotes	nM
T3875	13698-13700	IN	denotes	of
T3876	13701-13716	NN	denotes	oligonucleotide
T3877	13717-13724	NNS	denotes	primers
T3878	13724-13725	.	denotes	.
T3879	13725-13832	sentence	denotes	Initial steps of RT-PCR were two minutes at 50°C for UNG activation, followed by a 15 minute hold at 95°C.
T3880	13726-13733	JJ	denotes	Initial
T3881	13734-13739	NNS	denotes	steps
T3882	13750-13754	VBD	denotes	were
T3883	13740-13742	IN	denotes	of
T3884	13743-13745	NN	denotes	RT
T3885	13746-13749	NN	denotes	PCR
T3886	13745-13746	HYPH	denotes	-
T3887	13755-13758	CD	denotes	two
T3888	13759-13766	NNS	denotes	minutes
T3889	13767-13769	IN	denotes	at
T3890	13770-13772	CD	denotes	50
T3891	13772-13774	NN	denotes	°C
T3892	13775-13778	IN	denotes	for
T3893	13779-13782	NN	denotes	UNG
T3894	13783-13793	NN	denotes	activation
T3895	13793-13795	,	denotes	,
T3896	13795-13803	VBN	denotes	followed
T3897	13804-13806	IN	denotes	by
T3898	13807-13808	DT	denotes	a
T3899	13819-13823	NN	denotes	hold
T3900	13809-13811	CD	denotes	15
T3901	13812-13818	NN	denotes	minute
T3902	13824-13826	IN	denotes	at
T3903	13827-13829	CD	denotes	95
T3904	13829-13831	NN	denotes	°C
T3905	13831-13832	.	denotes	.
T3906	13832-13939	sentence	denotes	Cycles (n = 40) consisted of a 15 second melt at 95°C, followed by a 1 minute annealing/extension at 60°C.
T3907	13833-13839	NNS	denotes	Cycles
T3908	13849-13858	VBD	denotes	consisted
T3909	13840-13841	-LRB-	denotes	(
T3910	13845-13847	CD	denotes	40
T3911	13841-13842	NN	denotes	n
T3912	13843-13844	SYM	denotes	=
T3913	13847-13848	-RRB-	denotes	)
T3914	13859-13861	IN	denotes	of
T3915	13862-13863	DT	denotes	a
T3916	13874-13878	NN	denotes	melt
T3917	13864-13866	CD	denotes	15
T3918	13867-13873	JJ	denotes	second
T3919	13879-13881	IN	denotes	at
T3920	13882-13884	CD	denotes	95
T3921	13884-13886	NN	denotes	°C
T3922	13886-13888	,	denotes	,
T3923	13888-13896	VBN	denotes	followed
T3924	13897-13899	IN	denotes	by
T3925	13900-13901	DT	denotes	a
T3926	13904-13910	NN	denotes	minute
T3927	13902-13903	CD	denotes	1
T3928	13911-13920	NN	denotes	annealing
T3929	13921-13930	NN	denotes	extension
T3930	13920-13921	HYPH	denotes	/
T3931	13931-13933	IN	denotes	at
T3932	13934-13936	CD	denotes	60
T3933	13936-13938	NN	denotes	°C
T3934	13938-13939	.	denotes	.
T3935	13939-13990	sentence	denotes	The final step was a 60°C incubation for 1 minute.
T3936	13940-13943	DT	denotes	The
T3937	13950-13954	NN	denotes	step
T3938	13944-13949	JJ	denotes	final
T3939	13955-13958	VBD	denotes	was
T3940	13959-13960	DT	denotes	a
T3941	13966-13976	NN	denotes	incubation
T3942	13961-13963	CD	denotes	60
T3943	13963-13965	NN	denotes	°C
T3944	13977-13980	IN	denotes	for
T3945	13981-13982	CD	denotes	1
T3946	13983-13989	NN	denotes	minute
T3947	13989-13990	.	denotes	.
T3948	13990-14155	sentence	denotes	At the end of the run, samples were heated to 95°C with a ramp time of 20 minutes to construct dissociation curves to ensure that single PCR products were obtained.
T3949	13991-13993	IN	denotes	At
T3950	14027-14033	VBN	denotes	heated
T3951	13994-13997	DT	denotes	the
T3952	13998-14001	NN	denotes	end
T3953	14002-14004	IN	denotes	of
T3954	14005-14008	DT	denotes	the
T3955	14009-14012	NN	denotes	run
T3956	14012-14014	,	denotes	,
T3957	14014-14021	NNS	denotes	samples
T3958	14022-14026	VBD	denotes	were
T3959	14034-14036	IN	denotes	to
T3960	14037-14039	CD	denotes	95
T3961	14039-14041	NN	denotes	°C
T3962	14042-14046	IN	denotes	with
T3963	14047-14048	DT	denotes	a
T3964	14054-14058	NN	denotes	time
T3965	14049-14053	NN	denotes	ramp
T3966	14059-14061	IN	denotes	of
T3967	14062-14064	CD	denotes	20
T3968	14065-14072	NNS	denotes	minutes
T3969	14073-14075	TO	denotes	to
T3970	14076-14085	VB	denotes	construct
T3971	14086-14098	NN	denotes	dissociation
T3972	14099-14105	NNS	denotes	curves
T3973	14106-14108	TO	denotes	to
T3974	14109-14115	VB	denotes	ensure
T3975	14116-14120	IN	denotes	that
T3976	14146-14154	VBN	denotes	obtained
T3977	14121-14127	JJ	denotes	single
T3978	14132-14140	NNS	denotes	products
T3979	14128-14131	NN	denotes	PCR
T3980	14141-14145	VBD	denotes	were
T3981	14154-14155	.	denotes	.
T3982	14155-14199	sentence	denotes	All reactions were performed in triplicate.
T3983	14156-14159	DT	denotes	All
T3984	14160-14169	NNS	denotes	reactions
T3985	14175-14184	VBN	denotes	performed
T3986	14170-14174	VBD	denotes	were
T3987	14185-14187	IN	denotes	in
T3988	14188-14198	NN	denotes	triplicate
T3989	14198-14199	.	denotes	.
T3990	14199-14288	sentence	denotes	Threshold cycle (CT) analysis of all samples was set at 0.5 relative fluorescence units.
T3991	14200-14209	NN	denotes	Threshold
T3992	14210-14215	NN	denotes	cycle
T3993	14221-14229	NN	denotes	analysis
T3994	14216-14217	-LRB-	denotes	(
T3995	14217-14219	NN	denotes	CT
T3996	14219-14220	-RRB-	denotes	)
T3997	14249-14252	VBN	denotes	set
T3998	14230-14232	IN	denotes	of
T3999	14233-14236	DT	denotes	all
T4000	14237-14244	NNS	denotes	samples
T4001	14245-14248	VBD	denotes	was
T4002	14253-14255	IN	denotes	at
T4003	14256-14259	CD	denotes	0.5
T4004	14282-14287	NNS	denotes	units
T4005	14260-14268	JJ	denotes	relative
T4006	14269-14281	NN	denotes	fluorescence
T4007	14287-14288	.	denotes	.
T4008	14288-14480	sentence	denotes	The relative quantities of message of genes of interest from the mouse liver samples used in real-time RT-PCR were normalized to cyclophilin to compensate for variations in input RNA amounts.
T4009	14289-14292	DT	denotes	The
T4010	14302-14312	NNS	denotes	quantities
T4011	14293-14301	JJ	denotes	relative
T4012	14404-14414	VBN	denotes	normalized
T4013	14313-14315	IN	denotes	of
T4014	14316-14323	NN	denotes	message
T4015	14324-14326	IN	denotes	of
T4016	14327-14332	NNS	denotes	genes
T4017	14333-14335	IN	denotes	of
T4018	14336-14344	NN	denotes	interest
T4019	14345-14349	IN	denotes	from
T4020	14350-14353	DT	denotes	the
T4021	14366-14373	NNS	denotes	samples
T4022	14354-14359	NN	denotes	mouse
T4023	14360-14365	NN	denotes	liver
T4024	14374-14378	VBN	denotes	used
T4025	14379-14381	IN	denotes	in
T4026	14382-14386	JJ	denotes	real
T4027	14387-14391	NN	denotes	time
T4028	14386-14387	HYPH	denotes	-
T4029	14395-14398	NN	denotes	PCR
T4030	14392-14394	NN	denotes	RT
T4031	14394-14395	HYPH	denotes	-
T4032	14399-14403	VBD	denotes	were
T4033	14415-14417	IN	denotes	to
T4034	14418-14429	NN	denotes	cyclophilin
T4035	14430-14432	TO	denotes	to
T4036	14433-14443	VB	denotes	compensate
T4037	14444-14447	IN	denotes	for
T4038	14448-14458	NNS	denotes	variations
T4039	14459-14461	IN	denotes	in
T4040	14462-14467	NN	denotes	input
T4041	14468-14471	NN	denotes	RNA
T4042	14472-14479	NNS	denotes	amounts
T4043	14479-14480	.	denotes	.
T4044	14480-14554	sentence	denotes	The data were analyzed using the comparative threshold cycle method (CT).
T4045	14481-14484	DT	denotes	The
T4046	14485-14489	NNS	denotes	data
T4047	14495-14503	VBN	denotes	analyzed
T4048	14490-14494	VBD	denotes	were
T4049	14504-14509	VBG	denotes	using
T4050	14510-14513	DT	denotes	the
T4051	14542-14548	NN	denotes	method
T4052	14514-14525	JJ	denotes	comparative
T4053	14526-14535	NN	denotes	threshold
T4054	14536-14541	NN	denotes	cycle
T4055	14549-14550	-LRB-	denotes	(
T4056	14550-14552	NN	denotes	CT
T4057	14552-14553	-RRB-	denotes	)
T4058	14553-14554	.	denotes	.
T4059	14554-14749	sentence	denotes	Briefly, the CT values are averaged, from the triplicates defining a Δ-CT value calculated by taking the average CT of the gene of interest and subtracting it from the average CT of cyclophilin.
T4060	14555-14562	RB	denotes	Briefly
T4061	14582-14590	VBN	denotes	averaged
T4062	14562-14564	,	denotes	,
T4063	14564-14567	DT	denotes	the
T4064	14571-14577	NNS	denotes	values
T4065	14568-14570	NN	denotes	CT
T4066	14578-14581	VBP	denotes	are
T4067	14590-14592	,	denotes	,
T4068	14592-14596	IN	denotes	from
T4069	14597-14600	DT	denotes	the
T4070	14601-14612	NNS	denotes	triplicates
T4071	14613-14621	VBG	denotes	defining
T4072	14622-14623	DT	denotes	a
T4073	14629-14634	NN	denotes	value
T4074	14624-14625	NN	denotes	Δ
T4075	14626-14628	NN	denotes	CT
T4076	14625-14626	HYPH	denotes	-
T4077	14635-14645	VBN	denotes	calculated
T4078	14646-14648	IN	denotes	by
T4079	14649-14655	VBG	denotes	taking
T4080	14656-14659	DT	denotes	the
T4081	14668-14670	NN	denotes	CT
T4082	14660-14667	JJ	denotes	average
T4083	14671-14673	IN	denotes	of
T4084	14674-14677	DT	denotes	the
T4085	14678-14682	NN	denotes	gene
T4086	14683-14685	IN	denotes	of
T4087	14686-14694	NN	denotes	interest
T4088	14695-14698	CC	denotes	and
T4089	14699-14710	VBG	denotes	subtracting
T4090	14711-14713	PRP	denotes	it
T4091	14714-14718	IN	denotes	from
T4092	14719-14722	DT	denotes	the
T4093	14731-14733	NN	denotes	CT
T4094	14723-14730	JJ	denotes	average
T4095	14734-14736	IN	denotes	of
T4096	14737-14748	NN	denotes	cyclophilin
T4097	14748-14749	.	denotes	.
T4098	14749-14848	sentence	denotes	The ΔΔ-CT was calculated by subtracting the average Δ-CT(calibrator) values from the Δ-CT(sample).
T4099	14750-14753	DT	denotes	The
T4100	14757-14759	NN	denotes	CT
T4101	14754-14756	NN	denotes	ΔΔ
T4102	14756-14757	HYPH	denotes	-
T4103	14764-14774	VBN	denotes	calculated
T4104	14760-14763	VBD	denotes	was
T4105	14775-14777	IN	denotes	by
T4106	14778-14789	VBG	denotes	subtracting
T4107	14790-14793	DT	denotes	the
T4108	14819-14825	NNS	denotes	values
T4109	14794-14801	JJ	denotes	average
T4110	14802-14803	NN	denotes	Δ
T4111	14804-14806	NN	denotes	CT
T4112	14803-14804	HYPH	denotes	-
T4113	14806-14807	-LRB-	denotes	(
T4114	14807-14817	NN	denotes	calibrator
T4115	14817-14818	-RRB-	denotes	)
T4116	14826-14830	IN	denotes	from
T4117	14831-14834	DT	denotes	the
T4118	14837-14839	NN	denotes	CT
T4119	14835-14836	NN	denotes	Δ
T4120	14836-14837	HYPH	denotes	-
T4121	14839-14840	-LRB-	denotes	(
T4122	14840-14846	NN	denotes	sample
T4123	14846-14847	-RRB-	denotes	)
T4124	14847-14848	.	denotes	.
T4125	14848-14930	sentence	denotes	The relative quantification was then calculated by the expression 2-AverageΔΔ-CT.
T4126	14849-14852	DT	denotes	The
T4127	14862-14876	NN	denotes	quantification
T4128	14853-14861	JJ	denotes	relative
T4129	14886-14896	VBN	denotes	calculated
T4130	14877-14880	VBD	denotes	was
T4131	14881-14885	RB	denotes	then
T4132	14897-14899	IN	denotes	by
T4133	14900-14903	DT	denotes	the
T4134	14927-14929	NN	denotes	CT
T4135	14904-14914	NN	denotes	expression
T4136	14915-14916	CD	denotes	2
T4137	14916-14917	SYM	denotes	-
T4138	14917-14926	NN	denotes	AverageΔΔ
T4139	14926-14927	SYM	denotes	-
T4140	14929-14930	.	denotes	.
T4141	14930-15099	sentence	denotes	The mRNA quantity for the calibrator (wild type) was expressed as 1 and all other quantities were expressed as a fold difference relative to the calibrator (wild type).
T4142	14931-14934	DT	denotes	The
T4143	14940-14948	NN	denotes	quantity
T4144	14935-14939	NN	denotes	mRNA
T4145	14984-14993	VBN	denotes	expressed
T4146	14949-14952	IN	denotes	for
T4147	14953-14956	DT	denotes	the
T4148	14957-14967	NN	denotes	calibrator
T4149	14968-14969	-LRB-	denotes	(
T4150	14974-14978	NN	denotes	type
T4151	14969-14973	JJ	denotes	wild
T4152	14978-14979	-RRB-	denotes	)
T4154	14994-14996	IN	denotes	as
T4155	14997-14998	CD	denotes	1
T4156	14999-15002	CC	denotes	and
T4157	15003-15006	DT	denotes	all
T4158	15013-15023	NNS	denotes	quantities
T4159	15007-15012	JJ	denotes	other
T4160	15029-15038	VBN	denotes	expressed
T4161	15024-15028	VBD	denotes	were
T4162	15039-15041	IN	denotes	as
T4163	15042-15043	DT	denotes	a
T4164	15049-15059	NN	denotes	difference
T4165	15044-15048	NN	denotes	fold
T4166	15060-15068	JJ	denotes	relative
T4167	15069-15071	IN	denotes	to
T4168	15072-15075	DT	denotes	the
T4169	15076-15086	NN	denotes	calibrator
T4170	15087-15088	-LRB-	denotes	(
T4171	15093-15097	NN	denotes	type
T4172	15088-15092	JJ	denotes	wild
T4173	15097-15098	-RRB-	denotes	)
T4174	15098-15099	.	denotes	.
T4217	15101-15107	NN	denotes	Plasma
T4218	15108-15113	NN	denotes	lipid
T4219	15114-15122	NN	denotes	analysis
T4220	15123-15125	IN	denotes	by
T4221	15126-15130	JJ	denotes	fast
T4222	15131-15138	NN	denotes	protein
T4223	15146-15160	NN	denotes	chromatography
T4224	15139-15145	NN	denotes	liquid
T4225	15160-15444	sentence	denotes	Following 16 hrs of fasting, blood samples (n = 3) from each of the Abcg8+/+, Abcg8+/- and Abcg8-/- mice were collected from the retro-orbital venous plexus using heparinized capillary tubes under isoflurane anesthesia, and placed into precooled tubes containing 10 μl of 0.5 M EDTA.
T4226	15161-15170	VBG	denotes	Following
T4227	15271-15280	VBN	denotes	collected
T4228	15171-15173	CD	denotes	16
T4229	15174-15177	NNS	denotes	hrs
T4230	15178-15180	IN	denotes	of
T4231	15181-15188	NN	denotes	fasting
T4232	15188-15190	,	denotes	,
T4233	15190-15195	NN	denotes	blood
T4234	15196-15203	NNS	denotes	samples
T4235	15204-15205	-LRB-	denotes	(
T4236	15209-15210	CD	denotes	3
T4237	15205-15206	NN	denotes	n
T4238	15207-15208	SYM	denotes	=
T4239	15210-15211	-RRB-	denotes	)
T4240	15212-15216	IN	denotes	from
T4241	15217-15221	DT	denotes	each
T4242	15222-15224	IN	denotes	of
T4243	15225-15228	DT	denotes	the
T4244	15261-15265	NNS	denotes	mice
T4245	15229-15234	NN	denotes	Abcg8
T4246	15234-15235	SYM	denotes	+
T4247	15235-15236	HYPH	denotes	/
T4248	15236-15237	SYM	denotes	+
T4249	15237-15239	,	denotes	,
T4250	15239-15244	NN	denotes	Abcg8
T4251	15244-15245	SYM	denotes	+
T4252	15245-15246	HYPH	denotes	/
T4253	15246-15247	SYM	denotes	-
T4254	15248-15251	CC	denotes	and
T4255	15252-15257	NN	denotes	Abcg8
T4256	15257-15258	SYM	denotes	-
T4257	15258-15259	HYPH	denotes	/
T4258	15259-15260	SYM	denotes	-
T4259	15266-15270	VBD	denotes	were
T4260	15281-15285	IN	denotes	from
T4261	15286-15289	DT	denotes	the
T4262	15311-15317	NN	denotes	plexus
T4263	15290-15295	JJ	denotes	retro
T4264	15296-15303	JJ	denotes	orbital
T4265	15295-15296	HYPH	denotes	-
T4266	15304-15310	NN	denotes	venous
T4267	15318-15323	VBG	denotes	using
T4268	15324-15335	VBN	denotes	heparinized
T4269	15346-15351	NNS	denotes	tubes
T4270	15336-15345	JJ	denotes	capillary
T4271	15352-15357	IN	denotes	under
T4272	15358-15368	NN	denotes	isoflurane
T4273	15369-15379	NN	denotes	anesthesia
T4274	15379-15381	,	denotes	,
T4275	15381-15384	CC	denotes	and
T4276	15385-15391	VBN	denotes	placed
T4277	15392-15396	IN	denotes	into
T4278	15397-15406	JJ	denotes	precooled
T4279	15407-15412	NNS	denotes	tubes
T4280	15413-15423	VBG	denotes	containing
T4281	15424-15426	CD	denotes	10
T4282	15427-15429	NN	denotes	μl
T4283	15430-15432	IN	denotes	of
T4284	15433-15436	CD	denotes	0.5
T4285	15437-15438	NN	denotes	M
T4286	15439-15443	NN	denotes	EDTA
T4287	15443-15444	.	denotes	.
T4288	15444-15719	sentence	denotes	Equal volumes of plasma from each genotype were pooled and 100 μL of the pooled sample were injected and fractionated by fast protein liquid chromatography (FPLC) using a Superose 6 HR10/30 column (Pharmacia Biotech Inc., Piscataway, NJ, USA) and analyzed as described [27].
T4289	15445-15450	JJ	denotes	Equal
T4290	15451-15458	NNS	denotes	volumes
T4291	15493-15499	VBN	denotes	pooled
T4292	15459-15461	IN	denotes	of
T4293	15462-15468	NN	denotes	plasma
T4294	15469-15473	IN	denotes	from
T4295	15474-15478	DT	denotes	each
T4296	15479-15487	NN	denotes	genotype
T4297	15488-15492	VBD	denotes	were
T4298	15500-15503	CC	denotes	and
T4299	15504-15507	CD	denotes	100
T4300	15508-15510	NN	denotes	μL
T4301	15537-15545	VBN	denotes	injected
T4302	15511-15513	IN	denotes	of
T4303	15514-15517	DT	denotes	the
T4304	15525-15531	NN	denotes	sample
T4305	15518-15524	VBN	denotes	pooled
T4306	15532-15536	VBD	denotes	were
T4307	15546-15549	CC	denotes	and
T4308	15550-15562	VBN	denotes	fractionated
T4309	15563-15565	IN	denotes	by
T4310	15566-15570	JJ	denotes	fast
T4311	15571-15578	NN	denotes	protein
T4312	15586-15600	NN	denotes	chromatography
T4313	15579-15585	NN	denotes	liquid
T4314	15601-15602	-LRB-	denotes	(
T4315	15602-15606	NN	denotes	FPLC
T4316	15606-15607	-RRB-	denotes	)
T4317	15608-15613	VBG	denotes	using
T4318	15614-15615	DT	denotes	a
T4319	15635-15641	NN	denotes	column
T4320	15616-15624	NN	denotes	Superose
T4321	15625-15626	CD	denotes	6
T4322	15627-15631	NN	denotes	HR10
T4323	15631-15632	HYPH	denotes	/
T4324	15632-15634	CD	denotes	30
T4325	15642-15643	-LRB-	denotes	(
T4326	15661-15665	NNP	denotes	Inc.
T4327	15643-15652	NNP	denotes	Pharmacia
T4328	15653-15660	NNP	denotes	Biotech
T4329	15665-15667	,	denotes	,
T4330	15667-15677	NNP	denotes	Piscataway
T4331	15677-15679	,	denotes	,
T4332	15679-15681	NNP	denotes	NJ
T4333	15681-15683	,	denotes	,
T4334	15683-15686	NNP	denotes	USA
T4335	15686-15687	-RRB-	denotes	)
T4336	15688-15691	CC	denotes	and
T4337	15692-15700	VBN	denotes	analyzed
T4338	15701-15703	IN	denotes	as
T4339	15704-15713	VBN	denotes	described
T4340	15714-15715	-LRB-	denotes	[
T4341	15715-15717	CD	denotes	27
T4342	15717-15718	-RRB-	denotes	]
T4343	15718-15719	.	denotes	.
T4462	15721-15727	NN	denotes	Sterol
T4463	15728-15739	NN	denotes	composition
T4464	15740-15742	IN	denotes	of
T4465	15743-15749	NN	denotes	plasma
T4466	15750-15753	CC	denotes	and
T4467	15754-15761	NNS	denotes	tissues
T4468	15761-15981	sentence	denotes	Following a four-hour fast of 12-week-old mice fed on regular rodent chow diet; blood and tissues were collected under isofluorane anesthesia from each of the Abcg8+/+, Abcg8+/- and Abcg8-/- mice (n = 3 for each group).
T4469	15762-15771	VBG	denotes	Following
T4470	15865-15874	VBN	denotes	collected
T4471	15772-15773	DT	denotes	a
T4472	15784-15788	NN	denotes	fast
T4473	15774-15778	CD	denotes	four
T4474	15779-15783	NN	denotes	hour
T4475	15778-15779	HYPH	denotes	-
T4476	15789-15791	IN	denotes	of
T4477	15792-15794	CD	denotes	12
T4478	15795-15799	NN	denotes	week
T4479	15794-15795	HYPH	denotes	-
T4480	15800-15803	JJ	denotes	old
T4481	15799-15800	HYPH	denotes	-
T4482	15804-15808	NNS	denotes	mice
T4483	15809-15812	VBN	denotes	fed
T4484	15813-15815	IN	denotes	on
T4485	15816-15823	JJ	denotes	regular
T4486	15836-15840	NN	denotes	diet
T4487	15824-15830	NN	denotes	rodent
T4488	15831-15835	NN	denotes	chow
T4489	15840-15841	:	denotes	;
T4490	15842-15847	NN	denotes	blood
T4491	15848-15851	CC	denotes	and
T4492	15852-15859	NNS	denotes	tissues
T4493	15860-15864	VBD	denotes	were
T4494	15875-15880	IN	denotes	under
T4495	15881-15892	NN	denotes	isofluorane
T4496	15893-15903	NN	denotes	anesthesia
T4497	15904-15908	IN	denotes	from
T4498	15909-15913	DT	denotes	each
T4499	15914-15916	IN	denotes	of
T4500	15917-15920	DT	denotes	the
T4501	15953-15957	NNS	denotes	mice
T4502	15921-15926	NN	denotes	Abcg8
T4503	15926-15927	SYM	denotes	+
T4504	15927-15928	HYPH	denotes	/
T4505	15928-15929	SYM	denotes	+
T4506	15929-15931	,	denotes	,
T4507	15931-15936	NN	denotes	Abcg8
T4508	15936-15937	SYM	denotes	+
T4509	15937-15938	HYPH	denotes	/
T4510	15938-15939	SYM	denotes	-
T4511	15940-15943	CC	denotes	and
T4512	15944-15949	NN	denotes	Abcg8
T4513	15949-15950	SYM	denotes	-
T4514	15950-15951	HYPH	denotes	/
T4515	15951-15952	SYM	denotes	-
T4516	15958-15959	-LRB-	denotes	(
T4517	15963-15964	CD	denotes	3
T4518	15959-15960	NN	denotes	n
T4519	15961-15962	SYM	denotes	=
T4520	15965-15968	IN	denotes	for
T4521	15969-15973	DT	denotes	each
T4522	15974-15979	NN	denotes	group
T4523	15979-15980	-RRB-	denotes	)
T4524	15980-15981	.	denotes	.
T4525	15981-16065	sentence	denotes	Blood was collected from the retro-orbital venous plexus by heparinized glass tube.
T4526	15982-15987	NN	denotes	Blood
T4527	15992-16001	VBN	denotes	collected
T4528	15988-15991	VBD	denotes	was
T4529	16002-16006	IN	denotes	from
T4530	16007-16010	DT	denotes	the
T4531	16032-16038	NN	denotes	plexus
T4532	16011-16016	JJ	denotes	retro
T4533	16017-16024	JJ	denotes	orbital
T4534	16016-16017	HYPH	denotes	-
T4535	16025-16031	NN	denotes	venous
T4536	16039-16041	IN	denotes	by
T4537	16042-16053	VBN	denotes	heparinized
T4538	16060-16064	NN	denotes	tube
T4539	16054-16059	NN	denotes	glass
T4540	16064-16065	.	denotes	.
T4541	16065-16140	sentence	denotes	The animals were sacrificed by cervical dislocation for tissue collection.
T4542	16066-16069	DT	denotes	The
T4543	16070-16077	NNS	denotes	animals
T4544	16083-16093	VBN	denotes	sacrificed
T4545	16078-16082	VBD	denotes	were
T4546	16094-16096	IN	denotes	by
T4547	16097-16105	JJ	denotes	cervical
T4548	16106-16117	NN	denotes	dislocation
T4549	16118-16121	IN	denotes	for
T4550	16122-16128	NN	denotes	tissue
T4551	16129-16139	NN	denotes	collection
T4552	16139-16140	.	denotes	.
T4553	16140-16368	sentence	denotes	Plasma (100 μl) was saponified in 1 N NaOH for one hour, 1.5 mL water added and sterols extracted with three sequential portions of 1.5 mL ethyl acetate, containing 70 μg 5α-cholestane as an internal standard, pooled and dried.
T4554	16141-16147	NN	denotes	Plasma
T4555	16161-16171	VBN	denotes	saponified
T4556	16148-16149	-LRB-	denotes	(
T4557	16153-16155	NN	denotes	μl
T4558	16149-16152	CD	denotes	100
T4559	16155-16156	-RRB-	denotes	)
T4560	16157-16160	VBD	denotes	was
T4561	16172-16174	IN	denotes	in
T4562	16175-16176	CD	denotes	1
T4563	16177-16178	NN	denotes	N
T4564	16179-16183	NN	denotes	NaOH
T4565	16184-16187	IN	denotes	for
T4566	16188-16191	CD	denotes	one
T4567	16192-16196	NN	denotes	hour
T4568	16196-16198	,	denotes	,
T4569	16198-16201	CD	denotes	1.5
T4570	16202-16204	NN	denotes	mL
T4571	16205-16210	NN	denotes	water
T4572	16211-16216	VBD	denotes	added
T4573	16217-16220	CC	denotes	and
T4574	16221-16228	NNS	denotes	sterols
T4575	16229-16238	VBN	denotes	extracted
T4576	16239-16243	IN	denotes	with
T4577	16244-16249	CD	denotes	three
T4578	16261-16269	NNS	denotes	portions
T4579	16250-16260	JJ	denotes	sequential
T4580	16270-16272	IN	denotes	of
T4581	16273-16276	CD	denotes	1.5
T4582	16277-16279	NN	denotes	mL
T4583	16286-16293	NN	denotes	acetate
T4584	16280-16285	NN	denotes	ethyl
T4585	16293-16295	,	denotes	,
T4586	16295-16305	VBG	denotes	containing
T4587	16306-16308	CD	denotes	70
T4588	16309-16311	NN	denotes	μg
T4589	16315-16325	NN	denotes	cholestane
T4590	16312-16314	NN	denotes	5α
T4591	16314-16315	HYPH	denotes	-
T4592	16326-16328	IN	denotes	as
T4593	16329-16331	DT	denotes	an
T4594	16341-16349	NN	denotes	standard
T4595	16332-16340	JJ	denotes	internal
T4596	16349-16351	,	denotes	,
T4597	16351-16357	VBN	denotes	pooled
T4598	16358-16361	CC	denotes	and
T4599	16362-16367	VBN	denotes	dried
T4600	16367-16368	.	denotes	.
T4601	16368-16601	sentence	denotes	Mouse tissues (liver, spleen and brain) were weighed, homogenized in 1 mL phosphate buffer saline (PBS) with a dounce homogenizer (15 strokes at 500 rpm) and a small aliquot of the whole homogenate assayed for protein concentration.
T4602	16369-16374	NN	denotes	Mouse
T4603	16375-16382	NNS	denotes	tissues
T4604	16414-16421	VBN	denotes	weighed
T4605	16383-16384	-LRB-	denotes	(
T4606	16384-16389	NN	denotes	liver
T4607	16389-16391	,	denotes	,
T4608	16391-16397	NN	denotes	spleen
T4609	16398-16401	CC	denotes	and
T4610	16402-16407	NN	denotes	brain
T4611	16407-16408	-RRB-	denotes	)
T4612	16409-16413	VBD	denotes	were
T4613	16421-16423	,	denotes	,
T4614	16423-16434	VBN	denotes	homogenized
T4615	16435-16437	IN	denotes	in
T4616	16438-16439	CD	denotes	1
T4617	16440-16442	NN	denotes	mL
T4618	16443-16452	NN	denotes	phosphate
T4619	16453-16459	NN	denotes	buffer
T4620	16460-16466	NN	denotes	saline
T4621	16467-16468	-LRB-	denotes	(
T4622	16468-16471	NN	denotes	PBS
T4623	16471-16472	-RRB-	denotes	)
T4624	16473-16477	IN	denotes	with
T4625	16478-16479	DT	denotes	a
T4626	16487-16498	NN	denotes	homogenizer
T4627	16480-16486	NN	denotes	dounce
T4628	16499-16500	-LRB-	denotes	(
T4629	16503-16510	NNS	denotes	strokes
T4630	16500-16502	CD	denotes	15
T4631	16511-16513	IN	denotes	at
T4632	16514-16517	CD	denotes	500
T4633	16518-16521	NN	denotes	rpm
T4634	16521-16522	-RRB-	denotes	)
T4635	16523-16526	CC	denotes	and
T4636	16527-16528	DT	denotes	a
T4637	16535-16542	NN	denotes	aliquot
T4638	16529-16534	JJ	denotes	small
T4639	16543-16545	IN	denotes	of
T4640	16546-16549	DT	denotes	the
T4641	16556-16566	NN	denotes	homogenate
T4642	16550-16555	JJ	denotes	whole
T4643	16567-16574	VBN	denotes	assayed
T4644	16575-16578	IN	denotes	for
T4645	16579-16586	NN	denotes	protein
T4646	16587-16600	NN	denotes	concentration
T4647	16600-16601	.	denotes	.
T4648	16601-16695	sentence	denotes	Aliquots for sterol analysis from whole tissue homogenates were extracted as described above.
T4649	16602-16610	NNS	denotes	Aliquots
T4650	16666-16675	VBN	denotes	extracted
T4651	16611-16614	IN	denotes	for
T4652	16615-16621	NN	denotes	sterol
T4653	16622-16630	NN	denotes	analysis
T4654	16631-16635	IN	denotes	from
T4655	16636-16641	JJ	denotes	whole
T4656	16649-16660	NNS	denotes	homogenates
T4657	16642-16648	NN	denotes	tissue
T4658	16661-16665	VBD	denotes	were
T4659	16676-16678	IN	denotes	as
T4660	16679-16688	VBN	denotes	described
T4661	16689-16694	RB	denotes	above
T4662	16694-16695	.	denotes	.
T4663	16695-17030	sentence	denotes	Dried sterol samples were derivatized as trimethylsilylethers, redissolved in 10 μL hexane and 2 μL samples were injected into a capillary column (26 m length, 0.32 mm ID, 0.45 mm OD) coated with liquid CpWAX 57 CB (Chrompak, Bridgewater, NJ, USA) in a Hewlett-Packard Gas Chromatograph equipped with a flame ionization detector [28].
T4664	16696-16701	VBN	denotes	Dried
T4665	16709-16716	NNS	denotes	samples
T4666	16702-16708	NN	denotes	sterol
T4667	16722-16733	VBN	denotes	derivatized
T4668	16717-16721	VBD	denotes	were
T4669	16734-16736	IN	denotes	as
T4670	16737-16757	NNS	denotes	trimethylsilylethers
T4671	16757-16759	,	denotes	,
T4672	16759-16770	VBN	denotes	redissolved
T4673	16771-16773	IN	denotes	in
T4674	16774-16776	CD	denotes	10
T4675	16777-16779	NN	denotes	μL
T4676	16780-16786	NN	denotes	hexane
T4677	16787-16790	CC	denotes	and
T4678	16791-16792	CD	denotes	2
T4679	16793-16795	NN	denotes	μL
T4680	16796-16803	NNS	denotes	samples
T4681	16809-16817	VBN	denotes	injected
T4682	16804-16808	VBD	denotes	were
T4683	16818-16822	IN	denotes	into
T4684	16823-16824	DT	denotes	a
T4685	16835-16841	NN	denotes	column
T4686	16825-16834	JJ	denotes	capillary
T4687	16842-16843	-LRB-	denotes	(
T4688	16848-16854	NN	denotes	length
T4689	16843-16845	CD	denotes	26
T4690	16846-16847	NN	denotes	m
T4691	16854-16856	,	denotes	,
T4692	16856-16860	CD	denotes	0.32
T4693	16861-16863	NN	denotes	mm
T4694	16864-16866	NN	denotes	ID
T4695	16866-16868	,	denotes	,
T4696	16868-16872	CD	denotes	0.45
T4697	16873-16875	NN	denotes	mm
T4698	16876-16878	NN	denotes	OD
T4699	16878-16879	-RRB-	denotes	)
T4700	16880-16886	VBN	denotes	coated
T4701	16887-16891	IN	denotes	with
T4702	16892-16898	JJ	denotes	liquid
T4703	16908-16910	NN	denotes	CB
T4704	16899-16904	NN	denotes	CpWAX
T4705	16905-16907	CD	denotes	57
T4706	16911-16912	-LRB-	denotes	(
T4707	16912-16920	NNP	denotes	Chrompak
T4708	16920-16922	,	denotes	,
T4709	16922-16933	NNP	denotes	Bridgewater
T4710	16933-16935	,	denotes	,
T4711	16935-16937	NNP	denotes	NJ
T4712	16937-16939	,	denotes	,
T4713	16939-16942	NNP	denotes	USA
T4714	16942-16943	-RRB-	denotes	)
T4715	16944-16946	IN	denotes	in
T4716	16947-16948	DT	denotes	a
T4717	16969-16982	NNP	denotes	Chromatograph
T4718	16949-16956	NNP	denotes	Hewlett
T4719	16957-16964	NNP	denotes	Packard
T4720	16956-16957	HYPH	denotes	-
T4721	16965-16968	NNP	denotes	Gas
T4722	16983-16991	VBN	denotes	equipped
T4723	16992-16996	IN	denotes	with
T4724	16997-16998	DT	denotes	a
T4725	17016-17024	NN	denotes	detector
T4726	16999-17004	NN	denotes	flame
T4727	17005-17015	NN	denotes	ionization
T4728	17025-17026	-LRB-	denotes	[
T4729	17026-17028	CD	denotes	28
T4730	17028-17029	-RRB-	denotes	]
T4731	17029-17030	.	denotes	.
T4732	17030-17139	sentence	denotes	Concentrations of plasma and tissue sterols were reported as mg/dL and μg/g wet weight tissue, respectively.
T4733	17031-17045	NNS	denotes	Concentrations
T4734	17080-17088	VBN	denotes	reported
T4735	17046-17048	IN	denotes	of
T4736	17049-17055	NN	denotes	plasma
T4737	17067-17074	NNS	denotes	sterols
T4738	17056-17059	CC	denotes	and
T4739	17060-17066	NN	denotes	tissue
T4740	17075-17079	VBD	denotes	were
T4741	17089-17091	IN	denotes	as
T4742	17092-17094	NN	denotes	mg
T4743	17118-17124	NN	denotes	tissue
T4744	17094-17095	SYM	denotes	/
T4745	17095-17097	NN	denotes	dL
T4746	17098-17101	CC	denotes	and
T4747	17102-17104	NN	denotes	μg
T4748	17104-17105	SYM	denotes	/
T4749	17105-17106	NN	denotes	g
T4750	17107-17110	JJ	denotes	wet
T4751	17111-17117	NN	denotes	weight
T4752	17124-17126	,	denotes	,
T4753	17126-17138	RB	denotes	respectively
T4754	17138-17139	.	denotes	.
T4801	17272-17273	-LRB-	denotes	[
T4802	17279-17281	CD	denotes	30
T4803	17273-17275	CD	denotes	20
T4804	17275-17276	,	denotes	,
T4805	17276-17278	CD	denotes	29
T4806	17278-17279	,	denotes	,
T4807	17281-17282	-RRB-	denotes	]
T4808	17282-17283	.	denotes	.
T4809	17283-17357	sentence	denotes	In experiments, anti-Abcg5/sterolin-1 antibodies are labelled as follows:
T4810	17284-17286	IN	denotes	In
T4811	17337-17345	VBN	denotes	labelled
T4812	17287-17298	NNS	denotes	experiments
T4813	17298-17300	,	denotes	,
T4814	17300-17310	JJ	denotes	anti-Abcg5
T4815	17311-17319	NN	denotes	sterolin
T4816	17310-17311	HYPH	denotes	/
T4817	17322-17332	NNS	denotes	antibodies
T4818	17319-17320	HYPH	denotes	-
T4819	17320-17321	CD	denotes	1
T4820	17333-17336	VBP	denotes	are
T4821	17346-17348	IN	denotes	as
T4822	17349-17356	VBZ	denotes	follows
T4823	17356-17357	:	denotes	:
T4824	17357-17465	sentence	denotes	1) SC – from the Patel group, 2) AMC – from the AMC Liver Center group, and 3) UTSW – from the Hobbs group.
T4825	17358-17359	LS	denotes	1
T4826	17361-17363	NN	denotes	SC
T4827	17359-17360	-RRB-	denotes	)
T4828	17364-17365	:	denotes	–
T4829	17366-17370	IN	denotes	from
T4830	17371-17374	DT	denotes	the
T4831	17381-17386	NN	denotes	group
T4832	17375-17380	NNP	denotes	Patel
T4833	17386-17388	,	denotes	,
T4834	17388-17389	LS	denotes	2
T4835	17391-17394	NN	denotes	AMC
T4836	17389-17390	-RRB-	denotes	)
T4837	17395-17396	:	denotes	–
T4838	17397-17401	IN	denotes	from
T4839	17402-17405	DT	denotes	the
T4840	17423-17428	NN	denotes	group
T4841	17406-17409	NNP	denotes	AMC
T4842	17416-17422	NNP	denotes	Center
T4843	17410-17415	NNP	denotes	Liver
T4844	17428-17430	,	denotes	,
T4845	17430-17433	CC	denotes	and
T4846	17434-17435	LS	denotes	3
T4847	17437-17441	NN	denotes	UTSW
T4848	17435-17436	-RRB-	denotes	)
T4849	17442-17443	:	denotes	–
T4850	17444-17448	IN	denotes	from
T4851	17449-17452	DT	denotes	the
T4852	17459-17464	NN	denotes	group
T4853	17453-17458	NNP	denotes	Hobbs
T4854	17464-17465	.	denotes	.
T4905	17467-17475	NN	denotes	Membrane
T4906	17476-17483	NN	denotes	protein
T4907	17484-17496	NNS	denotes	preparations
T4908	17496-17737	sentence	denotes	Total membranes were prepared by taking approximately 500 mg of liver tissue cut into small pieces and homogenized in ice cold lysis buffer (5 mM Tris-pH7.5, 250 mM sucrose with protease inhibitors) by a dounce homogenizer times 10 strokes.
T4909	17497-17502	JJ	denotes	Total
T4910	17503-17512	NNS	denotes	membranes
T4911	17518-17526	VBN	denotes	prepared
T4912	17513-17517	VBD	denotes	were
T4913	17527-17529	IN	denotes	by
T4914	17530-17536	VBG	denotes	taking
T4915	17537-17550	RB	denotes	approximately
T4916	17551-17554	CD	denotes	500
T4917	17555-17557	NN	denotes	mg
T4918	17558-17560	IN	denotes	of
T4919	17561-17566	NN	denotes	liver
T4920	17567-17573	NN	denotes	tissue
T4921	17574-17577	VBD	denotes	cut
T4922	17578-17582	IN	denotes	into
T4923	17583-17588	JJ	denotes	small
T4924	17589-17595	NNS	denotes	pieces
T4925	17596-17599	CC	denotes	and
T4926	17600-17611	VBN	denotes	homogenized
T4927	17612-17614	IN	denotes	in
T4928	17615-17618	NN	denotes	ice
T4929	17619-17623	JJ	denotes	cold
T4930	17630-17636	NN	denotes	buffer
T4931	17624-17629	NN	denotes	lysis
T4932	17637-17638	-LRB-	denotes	(
T4933	17643-17647	NN	denotes	Tris
T4934	17638-17639	CD	denotes	5
T4935	17640-17642	NN	denotes	mM
T4936	17647-17648	HYPH	denotes	-
T4937	17648-17650	NN	denotes	pH
T4938	17650-17653	CD	denotes	7.5
T4939	17653-17655	,	denotes	,
T4940	17655-17658	CD	denotes	250
T4941	17659-17661	NN	denotes	mM
T4942	17662-17669	NN	denotes	sucrose
T4943	17670-17674	IN	denotes	with
T4944	17675-17683	NN	denotes	protease
T4945	17684-17694	NNS	denotes	inhibitors
T4946	17694-17695	-RRB-	denotes	)
T4947	17696-17698	IN	denotes	by
T4948	17699-17700	DT	denotes	a
T4949	17708-17719	NN	denotes	homogenizer
T4950	17701-17707	NN	denotes	dounce
T4951	17720-17725	IN	denotes	times
T4952	17726-17728	CD	denotes	10
T4953	17729-17736	NNS	denotes	strokes
T4954	17736-17737	.	denotes	.
T4955	17737-17815	sentence	denotes	Samples were then subjected to centrifugation – 1300 g for 10 minutes at 4°C.
T4956	17738-17745	NNS	denotes	Samples
T4957	17756-17765	VBN	denotes	subjected
T4958	17746-17750	VBD	denotes	were
T4959	17751-17755	RB	denotes	then
T4960	17766-17768	IN	denotes	to
T4961	17769-17783	NN	denotes	centrifugation
T4962	17784-17785	:	denotes	–
T4963	17786-17790	CD	denotes	1300
T4964	17791-17792	NN	denotes	g
T4965	17793-17796	IN	denotes	for
T4966	17797-17799	CD	denotes	10
T4967	17800-17807	NNS	denotes	minutes
T4968	17808-17810	IN	denotes	at
T4969	17811-17812	CD	denotes	4
T4970	17812-17814	NN	denotes	°C
T4971	17814-17815	.	denotes	.
T4972	17815-17979	sentence	denotes	The supernant was saved and the pellet was resuspended and again subjected to dounce homogenization times 10 strokes then centrifuged 1000 g for 10 minutes at 4°C.
T4973	17816-17819	DT	denotes	The
T4974	17820-17829	NN	denotes	supernant
T4975	17834-17839	VBN	denotes	saved
T4976	17830-17833	VBD	denotes	was
T4977	17840-17843	CC	denotes	and
T4978	17844-17847	DT	denotes	the
T4979	17848-17854	NN	denotes	pellet
T4980	17859-17870	VBN	denotes	resuspended
T4981	17855-17858	VBD	denotes	was
T4982	17871-17874	CC	denotes	and
T4983	17875-17880	RB	denotes	again
T4984	17881-17890	VBN	denotes	subjected
T4985	17891-17893	IN	denotes	to
T4986	17894-17900	NN	denotes	dounce
T4987	17901-17915	NN	denotes	homogenization
T4988	17916-17921	IN	denotes	times
T4989	17922-17924	CD	denotes	10
T4990	17925-17932	NNS	denotes	strokes
T4991	17933-17937	RB	denotes	then
T4992	17938-17949	VBD	denotes	centrifuged
T4993	17950-17954	CD	denotes	1000
T4994	17955-17956	NN	denotes	g
T4995	17957-17960	IN	denotes	for
T4996	17961-17963	CD	denotes	10
T4997	17964-17971	NNS	denotes	minutes
T4998	17972-17974	IN	denotes	at
T4999	17975-17976	CD	denotes	4
T5000	17976-17978	NN	denotes	°C
T5001	17978-17979	.	denotes	.
T5002	17979-18070	sentence	denotes	This step was repeated twice each time with the supernant being collected and kept on ice.
T5003	17980-17984	DT	denotes	This
T5004	17985-17989	NN	denotes	step
T5005	17994-18002	VBN	denotes	repeated
T5006	17990-17993	VBD	denotes	was
T5007	18003-18008	RB	denotes	twice
T5008	18014-18018	NN	denotes	time
T5009	18009-18013	DT	denotes	each
T5010	18019-18023	IN	denotes	with
T5011	18024-18027	DT	denotes	the
T5012	18028-18037	NN	denotes	supernant
T5013	18044-18053	VBN	denotes	collected
T5014	18038-18043	VBG	denotes	being
T5015	18054-18057	CC	denotes	and
T5016	18058-18062	VBN	denotes	kept
T5017	18063-18065	IN	denotes	on
T5018	18066-18069	NN	denotes	ice
T5019	18069-18070	.	denotes	.
T5020	18070-18175	sentence	denotes	The supernants were then pooled and subjected to ultracentrifugation at 100,000 g for 60 minutes at 4°C.
T5021	18071-18074	DT	denotes	The
T5022	18075-18085	NNS	denotes	supernants
T5023	18096-18102	VBN	denotes	pooled
T5024	18086-18090	VBD	denotes	were
T5025	18091-18095	RB	denotes	then
T5026	18103-18106	CC	denotes	and
T5027	18107-18116	VBN	denotes	subjected
T5028	18117-18119	IN	denotes	to
T5029	18120-18139	NN	denotes	ultracentrifugation
T5030	18140-18142	IN	denotes	at
T5031	18143-18150	CD	denotes	100,000
T5032	18151-18152	NN	denotes	g
T5033	18153-18156	IN	denotes	for
T5034	18157-18159	CD	denotes	60
T5035	18160-18167	NNS	denotes	minutes
T5036	18168-18170	IN	denotes	at
T5037	18171-18172	CD	denotes	4
T5038	18172-18174	NN	denotes	°C
T5039	18174-18175	.	denotes	.
T5040	18175-18249	sentence	denotes	The supernant was removed and the pellet was resuspended in lysis buffer.
T5041	18176-18179	DT	denotes	The
T5042	18180-18189	NN	denotes	supernant
T5043	18194-18201	VBN	denotes	removed
T5044	18190-18193	VBD	denotes	was
T5045	18202-18205	CC	denotes	and
T5046	18206-18209	DT	denotes	the
T5047	18210-18216	NN	denotes	pellet
T5048	18221-18232	VBN	denotes	resuspended
T5049	18217-18220	VBD	denotes	was
T5051	18236-18241	NN	denotes	lysis
T5052	18242-18248	NN	denotes	buffer
T5053	18248-18249	.	denotes	.
T5054	18249-18310	sentence	denotes	Plasma membranes were prepared as previously described [31].
T5055	18250-18256	NN	denotes	Plasma
T5056	18257-18266	NNS	denotes	membranes
T5057	18272-18280	VBN	denotes	prepared
T5058	18267-18271	VBD	denotes	were
T5059	18281-18283	IN	denotes	as
T5060	18295-18304	VBN	denotes	described
T5061	18284-18294	RB	denotes	previously
T5062	18305-18306	-LRB-	denotes	[
T5063	18306-18308	CD	denotes	31
T5064	18308-18309	-RRB-	denotes	]
T5065	18309-18310	.	denotes	.
T5066	18310-18455	sentence	denotes	Protein sample concentrations were then determined by Bio-Rad protein colormetric assay per manufacturer's protocol (BioRad, Hercules, CA, USA).
T5067	18311-18318	NN	denotes	Protein
T5068	18319-18325	NN	denotes	sample
T5069	18326-18340	NNS	denotes	concentrations
T5070	18351-18361	VBN	denotes	determined
T5071	18341-18345	VBD	denotes	were
T5072	18346-18350	RB	denotes	then
T5073	18362-18364	IN	denotes	by
T5074	18365-18368	NNP	denotes	Bio
T5075	18369-18372	NNP	denotes	Rad
T5076	18368-18369	HYPH	denotes	-
T5077	18393-18398	NN	denotes	assay
T5078	18373-18380	NN	denotes	protein
T5079	18381-18392	JJ	denotes	colormetric
T5080	18399-18402	IN	denotes	per
T5081	18403-18415	NN	denotes	manufacturer
T5082	18418-18426	NN	denotes	protocol
T5083	18415-18417	POS	denotes	's
T5084	18427-18428	-LRB-	denotes	(
T5085	18428-18434	NNP	denotes	BioRad
T5086	18434-18436	,	denotes	,
T5087	18436-18444	NNP	denotes	Hercules
T5088	18444-18446	,	denotes	,
T5089	18446-18448	NNP	denotes	CA
T5090	18448-18450	,	denotes	,
T5091	18450-18453	NNP	denotes	USA
T5092	18453-18454	-RRB-	denotes	)
T5093	18454-18455	.	denotes	.
T5094	18455-18529	sentence	denotes	50 μg of membrane proteins were then resolved on 7.5% SDS acrylamide gel.
T5095	18456-18458	CD	denotes	50
T5096	18459-18461	NN	denotes	μg
T5097	18493-18501	VBN	denotes	resolved
T5098	18462-18464	IN	denotes	of
T5099	18465-18473	NN	denotes	membrane
T5100	18474-18482	NN	denotes	proteins
T5101	18483-18487	VBD	denotes	were
T5102	18488-18492	RB	denotes	then
T5103	18502-18504	IN	denotes	on
T5104	18505-18508	CD	denotes	7.5
T5105	18508-18509	NN	denotes	%
T5106	18525-18528	NN	denotes	gel
T5107	18510-18513	NN	denotes	SDS
T5108	18514-18524	NN	denotes	acrylamide
T5109	18528-18529	.	denotes	.
T5166	18531-18545	NN	denotes	Immunoblotting
T5167	18545-18621	sentence	denotes	Proteins resolved by SDS-PAGE were transferred to nitrocellulose membranes.
T5168	18546-18554	NNS	denotes	Proteins
T5169	18581-18592	VBN	denotes	transferred
T5170	18555-18563	VBN	denotes	resolved
T5171	18564-18566	IN	denotes	by
T5172	18567-18570	NN	denotes	SDS
T5173	18571-18575	NN	denotes	PAGE
T5174	18570-18571	HYPH	denotes	-
T5175	18576-18580	VBD	denotes	were
T5176	18593-18595	IN	denotes	to
T5177	18596-18610	NN	denotes	nitrocellulose
T5178	18611-18620	NNS	denotes	membranes
T5179	18620-18621	.	denotes	.
T5180	18621-18733	sentence	denotes	Membranes were then blocked for one hour in 5% dry milk in PBS-T (Phosphate Buffered saline and 0.1% Tween 20).
T5181	18622-18631	NNS	denotes	Membranes
T5182	18642-18649	VBN	denotes	blocked
T5183	18632-18636	VBD	denotes	were
T5184	18637-18641	RB	denotes	then
T5185	18650-18653	IN	denotes	for
T5186	18654-18657	CD	denotes	one
T5187	18658-18662	NN	denotes	hour
T5188	18663-18665	IN	denotes	in
T5189	18666-18667	CD	denotes	5
T5190	18667-18668	NN	denotes	%
T5191	18673-18677	NN	denotes	milk
T5192	18669-18672	JJ	denotes	dry
T5193	18678-18680	IN	denotes	in
T5194	18681-18684	NN	denotes	PBS
T5195	18685-18686	NN	denotes	T
T5196	18684-18685	HYPH	denotes	-
T5197	18687-18688	-LRB-	denotes	(
T5198	18688-18697	NN	denotes	Phosphate
T5199	18698-18706	VBN	denotes	Buffered
T5200	18707-18713	NN	denotes	saline
T5201	18714-18717	CC	denotes	and
T5202	18718-18721	CD	denotes	0.1
T5203	18721-18722	NN	denotes	%
T5204	18723-18728	NN	denotes	Tween
T5205	18729-18731	CD	denotes	20
T5206	18731-18732	-RRB-	denotes	)
T5207	18732-18733	.	denotes	.
T5208	18733-18830	sentence	denotes	Blots were then probed with primary antibody against Abcg5 in 5% milk in PBS-T overnight at 4°C.
T5209	18734-18739	NNS	denotes	Blots
T5210	18750-18756	VBN	denotes	probed
T5211	18740-18744	VBD	denotes	were
T5212	18745-18749	RB	denotes	then
T5213	18757-18761	IN	denotes	with
T5214	18762-18769	JJ	denotes	primary
T5215	18770-18778	NN	denotes	antibody
T5216	18779-18786	IN	denotes	against
T5217	18787-18792	NN	denotes	Abcg5
T5218	18793-18795	IN	denotes	in
T5219	18796-18797	CD	denotes	5
T5220	18797-18798	NN	denotes	%
T5221	18799-18803	NN	denotes	milk
T5222	18804-18806	IN	denotes	in
T5223	18807-18810	NN	denotes	PBS
T5224	18811-18812	NN	denotes	T
T5225	18810-18811	HYPH	denotes	-
T5226	18813-18822	RB	denotes	overnight
T5227	18823-18825	IN	denotes	at
T5228	18826-18827	CD	denotes	4
T5229	18827-18829	NN	denotes	°C
T5230	18829-18830	.	denotes	.
T5231	18830-19061	sentence	denotes	The blot was then washed three times for five minutes in TBS-T (Tris Buffered Saline/0.1% Tween-20) with 150 mM NaCl, and secondarily stained with goat-anti-rabbit conjugated HRP 1:10,000 dilution in 5% milk in PBS-T for one hour.
T5232	18831-18834	DT	denotes	The
T5233	18835-18839	NN	denotes	blot
T5234	18849-18855	VBN	denotes	washed
T5235	18840-18843	VBD	denotes	was
T5236	18844-18848	RB	denotes	then
T5237	18856-18861	CD	denotes	three
T5238	18862-18867	NNS	denotes	times
T5239	18868-18871	IN	denotes	for
T5240	18872-18876	CD	denotes	five
T5241	18877-18884	NNS	denotes	minutes
T5242	18885-18887	IN	denotes	in
T5243	18888-18891	NN	denotes	TBS
T5244	18892-18893	NN	denotes	T
T5245	18891-18892	HYPH	denotes	-
T5246	18894-18895	-LRB-	denotes	(
T5247	18895-18899	NN	denotes	Tris
T5248	18900-18908	VBN	denotes	Buffered
T5249	18909-18915	NN	denotes	Saline
T5250	18915-18916	HYPH	denotes	/
T5251	18916-18919	CD	denotes	0.1
T5252	18919-18920	NN	denotes	%
T5253	18927-18929	NN	denotes	20
T5254	18921-18926	NN	denotes	Tween
T5255	18926-18927	HYPH	denotes	-
T5256	18929-18930	-RRB-	denotes	)
T5257	18931-18935	IN	denotes	with
T5258	18936-18939	CD	denotes	150
T5259	18940-18942	NN	denotes	mM
T5260	18943-18947	NN	denotes	NaCl
T5261	18947-18949	,	denotes	,
T5262	18949-18952	CC	denotes	and
T5263	18953-18964	RB	denotes	secondarily
T5264	18965-18972	VBN	denotes	stained
T5265	18973-18977	IN	denotes	with
T5266	18978-18982	NN	denotes	goat
T5267	18983-18994	NN	denotes	anti-rabbit
T5268	18982-18983	HYPH	denotes	-
T5269	18995-19005	VBN	denotes	conjugated
T5270	19019-19027	NN	denotes	dilution
T5271	19006-19009	NN	denotes	HRP
T5272	19010-19011	CD	denotes	1
T5273	19011-19012	SYM	denotes	:
T5274	19012-19018	CD	denotes	10,000
T5275	19028-19030	IN	denotes	in
T5276	19031-19032	CD	denotes	5
T5277	19032-19033	NN	denotes	%
T5278	19034-19038	NN	denotes	milk
T5279	19039-19041	IN	denotes	in
T5280	19042-19045	NN	denotes	PBS
T5281	19046-19047	NN	denotes	T
T5282	19045-19046	HYPH	denotes	-
T5283	19048-19051	IN	denotes	for
T5284	19052-19055	CD	denotes	one
T5285	19056-19060	NN	denotes	hour
T5286	19060-19061	.	denotes	.
T5287	19061-19310	sentence	denotes	The blot was again washed three times for five minutes in TBS-T with 150 mM NaCl then covered in Western Lightning® Chemiluminescence Reagent Plus (PerkinElmer Life Sciences, Inc. Boston, MA, USA), wrapped in plastic, exposed to film and developed.
T5288	19062-19065	DT	denotes	The
T5289	19066-19070	NN	denotes	blot
T5290	19081-19087	VBN	denotes	washed
T5291	19071-19074	VBD	denotes	was
T5292	19075-19080	RB	denotes	again
T5293	19088-19093	CD	denotes	three
T5294	19094-19099	NNS	denotes	times
T5295	19100-19103	IN	denotes	for
T5296	19104-19108	CD	denotes	five
T5297	19109-19116	NNS	denotes	minutes
T5298	19117-19119	IN	denotes	in
T5299	19120-19123	NN	denotes	TBS
T5300	19124-19125	NN	denotes	T
T5301	19123-19124	HYPH	denotes	-
T5302	19126-19130	IN	denotes	with
T5303	19131-19134	CD	denotes	150
T5304	19135-19137	NN	denotes	mM
T5305	19138-19142	NN	denotes	NaCl
T5306	19143-19147	RB	denotes	then
T5307	19148-19155	VBN	denotes	covered
T5308	19156-19158	IN	denotes	in
T5309	19159-19166	NNP	denotes	Western
T5310	19167-19177	NNP	denotes	Lightning®
T5311	19204-19208	NNP	denotes	Plus
T5312	19178-19195	NNP	denotes	Chemiluminescence
T5313	19196-19203	NNP	denotes	Reagent
T5314	19209-19210	-LRB-	denotes	(
T5315	19227-19235	NNPS	denotes	Sciences
T5316	19210-19221	NNP	denotes	PerkinElmer
T5317	19222-19226	NNP	denotes	Life
T5318	19235-19237	,	denotes	,
T5319	19237-19241	NNP	denotes	Inc.
T5320	19242-19248	NNP	denotes	Boston
T5321	19248-19250	,	denotes	,
T5322	19250-19252	NNP	denotes	MA
T5323	19252-19254	,	denotes	,
T5324	19254-19257	NNP	denotes	USA
T5325	19257-19258	-RRB-	denotes	)
T5326	19258-19260	,	denotes	,
T5327	19260-19267	VBN	denotes	wrapped
T5328	19268-19270	IN	denotes	in
T5329	19271-19278	NN	denotes	plastic
T5330	19278-19280	,	denotes	,
T5331	19280-19287	VBN	denotes	exposed
T5332	19288-19290	IN	denotes	to
T5333	19291-19295	NN	denotes	film
T5334	19296-19299	CC	denotes	and
T5335	19300-19309	VBN	denotes	developed
T5336	19309-19310	.	denotes	.
T5424	19312-19331	JJ	denotes	Immunohistochemical
T5425	19332-19340	NN	denotes	analysis
T5426	19340-19506	sentence	denotes	Snap-frozen liver and intestine tissue were cut to produce 8 μm thick frozen sections, air-dried for 30 minutes onto glass slides and stored at -80°C until required.
T5427	19341-19345	RB	denotes	Snap
T5428	19346-19352	VBN	denotes	frozen
T5429	19345-19346	HYPH	denotes	-
T5430	19373-19379	NN	denotes	tissue
T5431	19353-19358	NN	denotes	liver
T5432	19359-19362	CC	denotes	and
T5433	19363-19372	NN	denotes	intestine
T5434	19385-19388	VBN	denotes	cut
T5435	19380-19384	VBD	denotes	were
T5436	19389-19391	TO	denotes	to
T5437	19392-19399	VB	denotes	produce
T5438	19400-19401	CD	denotes	8
T5439	19402-19404	NN	denotes	μm
T5440	19405-19410	JJ	denotes	thick
T5441	19418-19426	NNS	denotes	sections
T5442	19411-19417	JJ	denotes	frozen
T5443	19426-19428	,	denotes	,
T5444	19428-19431	NN	denotes	air
T5445	19432-19437	VBN	denotes	dried
T5446	19431-19432	HYPH	denotes	-
T5447	19438-19441	IN	denotes	for
T5448	19442-19444	CD	denotes	30
T5449	19445-19452	NNS	denotes	minutes
T5450	19453-19457	IN	denotes	onto
T5451	19458-19463	NN	denotes	glass
T5452	19464-19470	NNS	denotes	slides
T5453	19471-19474	CC	denotes	and
T5454	19475-19481	VBN	denotes	stored
T5455	19482-19484	IN	denotes	at
T5456	19485-19486	SYM	denotes	-
T5457	19486-19488	CD	denotes	80
T5458	19488-19490	NN	denotes	°C
T5459	19491-19496	IN	denotes	until
T5460	19497-19505	VBN	denotes	required
T5461	19505-19506	.	denotes	.
T5462	19506-19662	sentence	denotes	The slides were stained with hematoxylin, rinsed three times with PBS, fixed for 10 min with cooled methanol at -20°C and then rinsed three times with PBS.
T5463	19507-19510	DT	denotes	The
T5464	19511-19517	NNS	denotes	slides
T5465	19523-19530	VBN	denotes	stained
T5466	19518-19522	VBD	denotes	were
T5467	19531-19535	IN	denotes	with
T5468	19536-19547	NN	denotes	hematoxylin
T5469	19547-19549	,	denotes	,
T5470	19549-19555	VBD	denotes	rinsed
T5471	19556-19561	CD	denotes	three
T5472	19562-19567	NNS	denotes	times
T5473	19568-19572	IN	denotes	with
T5474	19573-19576	NN	denotes	PBS
T5475	19576-19578	,	denotes	,
T5476	19578-19583	VBN	denotes	fixed
T5477	19584-19587	IN	denotes	for
T5478	19588-19590	CD	denotes	10
T5479	19591-19594	NN	denotes	min
T5480	19595-19599	IN	denotes	with
T5481	19600-19606	VBN	denotes	cooled
T5482	19607-19615	NN	denotes	methanol
T5483	19616-19618	IN	denotes	at
T5484	19619-19620	SYM	denotes	-
T5485	19620-19622	CD	denotes	20
T5486	19622-19624	NN	denotes	°C
T5487	19625-19628	CC	denotes	and
T5488	19629-19633	RB	denotes	then
T5489	19634-19640	VBD	denotes	rinsed
T5490	19641-19646	CD	denotes	three
T5491	19647-19652	NNS	denotes	times
T5492	19653-19657	IN	denotes	with
T5493	19658-19661	NN	denotes	PBS
T5494	19661-19662	.	denotes	.
T5495	19662-19860	sentence	denotes	Slides for antibody staining were initially blocked in PBS/0.1 M glycine/10% goat serum for 60 min at room temperature then incubated with anti-sterolin-1 antibody (1:15 dilution) overnight at 4°C.
T5496	19663-19669	NNS	denotes	Slides
T5497	19707-19714	VBN	denotes	blocked
T5498	19670-19673	IN	denotes	for
T5499	19674-19682	NN	denotes	antibody
T5500	19683-19691	NN	denotes	staining
T5501	19692-19696	VBD	denotes	were
T5502	19697-19706	RB	denotes	initially
T5503	19715-19717	IN	denotes	in
T5504	19718-19721	NN	denotes	PBS
T5505	19726-19727	NN	denotes	M
T5506	19721-19722	HYPH	denotes	/
T5507	19722-19725	CD	denotes	0.1
T5508	19745-19750	NN	denotes	serum
T5509	19728-19735	NN	denotes	glycine
T5510	19738-19739	NN	denotes	%
T5511	19735-19736	HYPH	denotes	/
T5512	19736-19738	CD	denotes	10
T5513	19740-19744	NN	denotes	goat
T5514	19751-19754	IN	denotes	for
T5515	19755-19757	CD	denotes	60
T5516	19758-19761	NN	denotes	min
T5517	19762-19764	IN	denotes	at
T5518	19765-19769	NN	denotes	room
T5519	19770-19781	NN	denotes	temperature
T5520	19782-19786	RB	denotes	then
T5521	19787-19796	VBN	denotes	incubated
T5522	19797-19801	IN	denotes	with
T5523	19802-19815	JJ	denotes	anti-sterolin
T5524	19818-19826	NN	denotes	antibody
T5525	19815-19816	HYPH	denotes	-
T5526	19816-19817	CD	denotes	1
T5527	19827-19828	-LRB-	denotes	(
T5528	19833-19841	NN	denotes	dilution
T5529	19828-19829	CD	denotes	1
T5530	19829-19830	SYM	denotes	:
T5531	19830-19832	CD	denotes	15
T5532	19841-19842	-RRB-	denotes	)
T5533	19843-19852	RB	denotes	overnight
T5534	19853-19855	IN	denotes	at
T5535	19856-19857	CD	denotes	4
T5536	19857-19859	NN	denotes	°C
T5537	19859-19860	.	denotes	.
T5538	19860-20114	sentence	denotes	The slides were washed with 1 × PBS and incubated with secondary antibody (goat-anti-rabbit IgG conjugated with rhodamine, 1:1000 dilution) for 30 min at room temperature, washed and then observed under an Olympus BX-5 confocal microscope with Fluoview.
T5539	19861-19864	DT	denotes	The
T5540	19865-19871	NNS	denotes	slides
T5541	19877-19883	VBN	denotes	washed
T5542	19872-19876	VBD	denotes	were
T5543	19884-19888	IN	denotes	with
T5544	19889-19890	CD	denotes	1
T5545	19893-19896	NN	denotes	PBS
T5546	19891-19892	SYM	denotes	×
T5547	19897-19900	CC	denotes	and
T5548	19901-19910	VBN	denotes	incubated
T5549	19911-19915	IN	denotes	with
T5550	19916-19925	JJ	denotes	secondary
T5551	19926-19934	NN	denotes	antibody
T5552	19935-19936	-LRB-	denotes	(
T5553	19991-19999	NN	denotes	dilution
T5554	19936-19940	NN	denotes	goat
T5555	19941-19952	NN	denotes	anti-rabbit
T5556	19940-19941	HYPH	denotes	-
T5557	19953-19956	NN	denotes	IgG
T5558	19957-19967	VBN	denotes	conjugated
T5559	19968-19972	IN	denotes	with
T5560	19973-19982	NN	denotes	rhodamine
T5561	19982-19984	,	denotes	,
T5562	19984-19985	CD	denotes	1
T5563	19985-19986	SYM	denotes	:
T5564	19986-19990	CD	denotes	1000
T5565	19999-20000	-RRB-	denotes	)
T5566	20001-20004	IN	denotes	for
T5567	20005-20007	CD	denotes	30
T5568	20008-20011	NN	denotes	min
T5569	20012-20014	IN	denotes	at
T5570	20015-20019	NN	denotes	room
T5571	20020-20031	NN	denotes	temperature
T5572	20031-20033	,	denotes	,
T5573	20033-20039	VBD	denotes	washed
T5574	20040-20043	CC	denotes	and
T5575	20044-20048	RB	denotes	then
T5576	20049-20057	VBN	denotes	observed
T5577	20058-20063	IN	denotes	under
T5578	20064-20066	DT	denotes	an
T5579	20089-20099	NN	denotes	microscope
T5580	20067-20074	NNP	denotes	Olympus
T5581	20075-20077	NN	denotes	BX
T5582	20077-20078	HYPH	denotes	-
T5583	20078-20079	CD	denotes	5
T5584	20080-20088	JJ	denotes	confocal
T5585	20100-20104	IN	denotes	with
T5586	20105-20113	NNP	denotes	Fluoview
T5587	20113-20114	.	denotes	.
T5588	20114-20417	sentence	denotes	The sections stained at the AMC Liver Center were fixed in 100% acetone for 30 min at room temperature, blocked in a solution of PBS/0.05% Tween-20/5% goat serum (GS) for one hour, then incubated with Anti-sterolin-1 (1:40 dilution) and anti-Bsep (1:200 dilution) diluted in PBS/Tween/GS, for one hour.
T5589	20115-20118	DT	denotes	The
T5590	20119-20127	NNS	denotes	sections
T5591	20165-20170	VBN	denotes	fixed
T5592	20128-20135	VBN	denotes	stained
T5593	20136-20138	IN	denotes	at
T5594	20139-20142	DT	denotes	the
T5595	20153-20159	NNP	denotes	Center
T5596	20143-20146	NNP	denotes	AMC
T5597	20147-20152	NNP	denotes	Liver
T5598	20160-20164	VBD	denotes	were
T5599	20171-20173	IN	denotes	in
T5600	20174-20177	CD	denotes	100
T5601	20177-20178	NN	denotes	%
T5602	20179-20186	NN	denotes	acetone
T5603	20187-20190	IN	denotes	for
T5604	20191-20193	CD	denotes	30
T5605	20194-20197	NN	denotes	min
T5606	20198-20200	IN	denotes	at
T5607	20201-20205	NN	denotes	room
T5608	20206-20217	NN	denotes	temperature
T5609	20217-20219	,	denotes	,
T5610	20219-20226	VBD	denotes	blocked
T5611	20227-20229	IN	denotes	in
T5612	20230-20231	DT	denotes	a
T5613	20232-20240	NN	denotes	solution
T5614	20241-20243	IN	denotes	of
T5615	20244-20247	NN	denotes	PBS
T5616	20252-20253	NN	denotes	%
T5617	20247-20248	HYPH	denotes	/
T5618	20248-20252	CD	denotes	0.05
T5619	20254-20259	NNP	denotes	Tween
T5620	20264-20265	NN	denotes	%
T5621	20259-20260	HYPH	denotes	-
T5622	20260-20262	CD	denotes	20
T5623	20262-20263	HYPH	denotes	/
T5624	20263-20264	CD	denotes	5
T5625	20266-20270	NN	denotes	goat
T5626	20271-20276	NN	denotes	serum
T5627	20277-20278	-LRB-	denotes	(
T5628	20278-20280	NN	denotes	GS
T5629	20280-20281	-RRB-	denotes	)
T5630	20282-20285	IN	denotes	for
T5631	20286-20289	CD	denotes	one
T5632	20290-20294	NN	denotes	hour
T5633	20294-20296	,	denotes	,
T5634	20296-20300	RB	denotes	then
T5635	20301-20310	VBN	denotes	incubated
T5636	20311-20315	IN	denotes	with
T5637	20316-20329	NN	denotes	Anti-sterolin
T5638	20329-20330	HYPH	denotes	-
T5639	20330-20331	CD	denotes	1
T5640	20332-20333	-LRB-	denotes	(
T5641	20338-20346	NN	denotes	dilution
T5642	20333-20334	CD	denotes	1
T5643	20334-20335	SYM	denotes	:
T5644	20335-20337	CD	denotes	40
T5645	20346-20347	-RRB-	denotes	)
T5646	20348-20351	CC	denotes	and
T5647	20352-20361	JJ	denotes	anti-Bsep
T5648	20362-20363	-LRB-	denotes	(
T5649	20369-20377	NN	denotes	dilution
T5650	20363-20364	CD	denotes	1
T5651	20364-20365	SYM	denotes	:
T5652	20365-20368	CD	denotes	200
T5653	20377-20378	-RRB-	denotes	)
T5654	20379-20386	VBN	denotes	diluted
T5655	20387-20389	IN	denotes	in
T5656	20390-20393	NN	denotes	PBS
T5657	20400-20402	NN	denotes	GS
T5658	20393-20394	HYPH	denotes	/
T5659	20394-20399	NN	denotes	Tween
T5660	20399-20400	HYPH	denotes	/
T5661	20402-20404	,	denotes	,
T5662	20404-20407	IN	denotes	for
T5663	20408-20411	CD	denotes	one
T5664	20412-20416	NN	denotes	hour
T5665	20416-20417	.	denotes	.
T5666	20417-20604	sentence	denotes	Thereafter, slides were washed 3 × 10 min in PBS/Tween and the secondary antibody (goat-anti-rabbit Alexa 488) was applied in 1:1000 dilution in PBST/GS for one hour at room temperature.
T5667	20418-20428	RB	denotes	Thereafter
T5668	20442-20448	VBN	denotes	washed
T5669	20428-20430	,	denotes	,
T5670	20430-20436	NNS	denotes	slides
T5671	20437-20441	VBD	denotes	were
T5672	20449-20450	CD	denotes	3
T5673	20456-20459	NN	denotes	min
T5674	20451-20452	SYM	denotes	×
T5675	20453-20455	CD	denotes	10
T5676	20460-20462	IN	denotes	in
T5677	20463-20466	NN	denotes	PBS
T5678	20467-20472	NN	denotes	Tween
T5679	20466-20467	HYPH	denotes	/
T5680	20473-20476	CC	denotes	and
T5681	20477-20480	DT	denotes	the
T5682	20491-20499	NN	denotes	antibody
T5683	20481-20490	JJ	denotes	secondary
T5684	20533-20540	VBN	denotes	applied
T5685	20500-20501	-LRB-	denotes	(
T5686	20501-20505	NN	denotes	goat
T5687	20506-20517	NN	denotes	anti-rabbit
T5688	20505-20506	HYPH	denotes	-
T5689	20518-20523	NNP	denotes	Alexa
T5690	20524-20527	CD	denotes	488
T5691	20527-20528	-RRB-	denotes	)
T5692	20529-20532	VBD	denotes	was
T5693	20541-20543	IN	denotes	in
T5694	20544-20545	CD	denotes	1
T5695	20551-20559	NN	denotes	dilution
T5696	20545-20546	SYM	denotes	:
T5697	20546-20550	CD	denotes	1000
T5698	20560-20562	IN	denotes	in
T5699	20563-20567	NN	denotes	PBST
T5700	20568-20570	NN	denotes	GS
T5701	20567-20568	HYPH	denotes	/
T5702	20571-20574	IN	denotes	for
T5703	20575-20578	CD	denotes	one
T5704	20579-20583	NN	denotes	hour
T5705	20584-20586	IN	denotes	at
T5706	20587-20591	NN	denotes	room
T5707	20592-20603	NN	denotes	temperature
T5708	20603-20604	.	denotes	.
T5709	20604-20683	sentence	denotes	After washing for 3 × 10 min the sections were mounted in Dapi/Dapco solution.
T5710	20605-20610	IN	denotes	After
T5711	20652-20659	VBN	denotes	mounted
T5712	20611-20618	VBG	denotes	washing
T5713	20619-20622	IN	denotes	for
T5714	20623-20624	CD	denotes	3
T5715	20630-20633	NN	denotes	min
T5716	20625-20626	SYM	denotes	×
T5717	20627-20629	CD	denotes	10
T5718	20634-20637	DT	denotes	the
T5719	20638-20646	NNS	denotes	sections
T5720	20647-20651	VBD	denotes	were
T5721	20660-20662	IN	denotes	in
T5722	20663-20667	NN	denotes	Dapi
T5723	20668-20673	NN	denotes	Dapco
T5724	20667-20668	HYPH	denotes	/
T5725	20674-20682	NN	denotes	solution
T5726	20682-20683	.	denotes	.
T5727	20683-20790	sentence	denotes	Fluorescence microscopy was done with a Leica DM RA2 microscope equipped with a Leica DC350F photo camera.
T5728	20684-20696	NN	denotes	Fluorescence
T5729	20697-20707	NN	denotes	microscopy
T5730	20712-20716	VBN	denotes	done
T5731	20708-20711	VBD	denotes	was
T5732	20717-20721	IN	denotes	with
T5733	20722-20723	DT	denotes	a
T5734	20737-20747	NN	denotes	microscope
T5735	20724-20729	NNP	denotes	Leica
T5736	20730-20732	NN	denotes	DM
T5737	20733-20736	NN	denotes	RA2
T5738	20748-20756	VBN	denotes	equipped
T5739	20757-20761	IN	denotes	with
T5740	20762-20763	DT	denotes	a
T5741	20783-20789	NN	denotes	camera
T5742	20764-20769	NNP	denotes	Leica
T5743	20770-20776	NN	denotes	DC350F
T5744	20777-20782	NN	denotes	photo
T5745	20789-20790	.	denotes	.
T5746	20790-21001	sentence	denotes	For Dapi staining, 359 nm (30.38 ms exposure) was used and for Abcg5 and Bsep staining, 514 nm (for both: 1654.34 ms exposure) was used and the pictures were analyzed and overlaid using FW4000 software (Leica).
T5747	20791-20794	IN	denotes	For
T5748	20841-20845	VBN	denotes	used
T5749	20795-20799	NNP	denotes	Dapi
T5750	20800-20808	NN	denotes	staining
T5751	20808-20810	,	denotes	,
T5752	20810-20813	CD	denotes	359
T5753	20814-20816	NN	denotes	nm
T5754	20817-20818	-LRB-	denotes	(
T5755	20827-20835	NN	denotes	exposure
T5756	20818-20823	CD	denotes	30.38
T5757	20824-20826	NN	denotes	ms
T5758	20835-20836	-RRB-	denotes	)
T5759	20837-20840	VBD	denotes	was
T5760	20846-20849	CC	denotes	and
T5761	20850-20853	IN	denotes	for
T5762	20922-20926	VBN	denotes	used
T5763	20854-20859	NN	denotes	Abcg5
T5764	20869-20877	NN	denotes	staining
T5765	20860-20863	CC	denotes	and
T5766	20864-20868	NN	denotes	Bsep
T5767	20877-20879	,	denotes	,
T5768	20879-20882	CD	denotes	514
T5769	20883-20885	NN	denotes	nm
T5770	20886-20887	-LRB-	denotes	(
T5771	20908-20916	NN	denotes	exposure
T5772	20887-20890	IN	denotes	for
T5773	20891-20895	DT	denotes	both
T5774	20895-20897	:	denotes	:
T5775	20897-20904	CD	denotes	1654.34
T5776	20905-20907	NN	denotes	ms
T5777	20916-20917	-RRB-	denotes	)
T5778	20918-20921	VBD	denotes	was
T5779	20927-20930	CC	denotes	and
T5780	20931-20934	DT	denotes	the
T5781	20935-20943	NNS	denotes	pictures
T5782	20949-20957	VBN	denotes	analyzed
T5783	20944-20948	VBD	denotes	were
T5784	20958-20961	CC	denotes	and
T5785	20962-20970	VBN	denotes	overlaid
T5786	20971-20976	VBG	denotes	using
T5787	20977-20983	NN	denotes	FW4000
T5788	20984-20992	NN	denotes	software
T5789	20993-20994	-LRB-	denotes	(
T5790	20994-20999	NNP	denotes	Leica
T5791	20999-21000	-RRB-	denotes	)
T5792	21000-21001	.	denotes	.
T5900	21003-21015	NN	denotes	Transfection
T5901	21016-21019	CC	denotes	and
T5902	21020-21034	NN	denotes	immunostaining
T5903	21035-21037	IN	denotes	of
T5904	21038-21041	NN	denotes	COS
T5905	21044-21049	NNS	denotes	cells
T5906	21041-21042	HYPH	denotes	-
T5907	21042-21043	CD	denotes	1
T5908	21049-21224	sentence	denotes	COS-1 cells were transiently transfected with pCMV-mouse Abcg5 and pCMV-mouse Abcg8 expression constructs using the Qiagen SuperFect reagent per protocol (Valencia, CA, USA).
T5909	21050-21053	NN	denotes	COS
T5910	21056-21061	NNS	denotes	cells
T5911	21053-21054	HYPH	denotes	-
T5912	21054-21055	CD	denotes	1
T5913	21079-21090	VBN	denotes	transfected
T5914	21062-21066	VBD	denotes	were
T5915	21067-21078	RB	denotes	transiently
T5916	21091-21095	IN	denotes	with
T5917	21096-21100	NN	denotes	pCMV
T5918	21101-21106	NN	denotes	mouse
T5919	21100-21101	HYPH	denotes	-
T5920	21107-21112	NN	denotes	Abcg5
T5921	21145-21155	NNS	denotes	constructs
T5922	21113-21116	CC	denotes	and
T5923	21117-21121	NN	denotes	pCMV
T5924	21122-21127	NN	denotes	mouse
T5925	21121-21122	HYPH	denotes	-
T5926	21128-21133	NN	denotes	Abcg8
T5927	21134-21144	NN	denotes	expression
T5928	21156-21161	VBG	denotes	using
T5929	21162-21165	DT	denotes	the
T5930	21183-21190	NN	denotes	reagent
T5931	21166-21172	NNP	denotes	Qiagen
T5932	21173-21182	NN	denotes	SuperFect
T5933	21191-21194	IN	denotes	per
T5934	21195-21203	NN	denotes	protocol
T5935	21204-21205	-LRB-	denotes	(
T5936	21205-21213	NNP	denotes	Valencia
T5937	21213-21215	,	denotes	,
T5938	21215-21217	NNP	denotes	CA
T5939	21217-21219	,	denotes	,
T5940	21219-21222	NNP	denotes	USA
T5941	21222-21223	-RRB-	denotes	)
T5942	21223-21224	.	denotes	.
T5943	21224-21464	sentence	denotes	Briefly, COS-1 cells were seeded onto a six-well plate the day prior to transfection to generate 60% confluence when grown at 37°C and 5% CO2 in DMEM supplemented with 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin and 1 × glutamine.
T5944	21225-21232	RB	denotes	Briefly
T5945	21251-21257	VBN	denotes	seeded
T5946	21232-21234	,	denotes	,
T5947	21234-21237	NN	denotes	COS
T5948	21240-21245	NNS	denotes	cells
T5949	21237-21238	HYPH	denotes	-
T5950	21238-21239	CD	denotes	1
T5951	21246-21250	VBD	denotes	were
T5952	21258-21262	IN	denotes	onto
T5953	21263-21264	DT	denotes	a
T5954	21274-21279	NN	denotes	plate
T5955	21265-21268	CD	denotes	six
T5956	21269-21273	NN	denotes	well
T5957	21268-21269	HYPH	denotes	-
T5958	21280-21283	DT	denotes	the
T5959	21284-21287	NN	denotes	day
T5960	21288-21293	JJ	denotes	prior
T5961	21294-21296	IN	denotes	to
T5962	21297-21309	NN	denotes	transfection
T5963	21310-21312	TO	denotes	to
T5964	21313-21321	VB	denotes	generate
T5965	21322-21324	CD	denotes	60
T5966	21324-21325	NN	denotes	%
T5967	21326-21336	NN	denotes	confluence
T5968	21337-21341	WRB	denotes	when
T5969	21342-21347	VBN	denotes	grown
T5970	21348-21350	IN	denotes	at
T5971	21351-21353	CD	denotes	37
T5972	21353-21355	NN	denotes	°C
T5973	21356-21359	CC	denotes	and
T5974	21360-21361	CD	denotes	5
T5975	21361-21362	NN	denotes	%
T5976	21363-21366	NN	denotes	CO2
T5977	21367-21369	IN	denotes	in
T5978	21370-21374	NN	denotes	DMEM
T5979	21375-21387	VBN	denotes	supplemented
T5980	21388-21392	IN	denotes	with
T5981	21393-21395	CD	denotes	10
T5982	21395-21396	NN	denotes	%
T5983	21397-21400	NN	denotes	FBS
T5984	21400-21402	,	denotes	,
T5985	21402-21405	CD	denotes	100
T5986	21406-21407	NN	denotes	U
T5987	21411-21421	NN	denotes	penicillin
T5988	21407-21408	SYM	denotes	/
T5989	21408-21410	NN	denotes	ml
T5990	21421-21423	,	denotes	,
T5991	21423-21426	CD	denotes	100
T5992	21427-21429	NN	denotes	μg
T5993	21433-21445	NN	denotes	streptomycin
T5994	21429-21430	SYM	denotes	/
T5995	21430-21432	NN	denotes	ml
T5996	21446-21449	CC	denotes	and
T5997	21450-21451	CD	denotes	1
T5998	21454-21463	NN	denotes	glutamine
T5999	21452-21453	SYM	denotes	×
T6000	21463-21464	.	denotes	.
T6001	21464-21574	sentence	denotes	On the day of transfection, 2 μg of DNA was mixed with Qiagen SuperFect reagent and incubated for 10 minutes.
T6002	21465-21467	IN	denotes	On
T6003	21509-21514	VBN	denotes	mixed
T6004	21468-21471	DT	denotes	the
T6005	21472-21475	NN	denotes	day
T6006	21476-21478	IN	denotes	of
T6007	21479-21491	NN	denotes	transfection
T6008	21491-21493	,	denotes	,
T6009	21493-21494	CD	denotes	2
T6010	21495-21497	NN	denotes	μg
T6011	21498-21500	IN	denotes	of
T6012	21501-21504	NN	denotes	DNA
T6013	21505-21508	VBD	denotes	was
T6014	21515-21519	IN	denotes	with
T6015	21520-21526	NNP	denotes	Qiagen
T6016	21527-21536	NN	denotes	SuperFect
T6017	21537-21544	NN	denotes	reagent
T6018	21545-21548	CC	denotes	and
T6019	21549-21558	VBN	denotes	incubated
T6020	21559-21562	IN	denotes	for
T6021	21563-21565	CD	denotes	10
T6022	21566-21573	NNS	denotes	minutes
T6023	21573-21574	.	denotes	.
T6024	21574-21673	sentence	denotes	This mixture was then added to serum-free media and placed on the cells to incubate for two hours.
T6025	21575-21579	DT	denotes	This
T6026	21580-21587	NN	denotes	mixture
T6027	21597-21602	VBN	denotes	added
T6028	21588-21591	VBD	denotes	was
T6029	21592-21596	RB	denotes	then
T6030	21603-21605	IN	denotes	to
T6031	21606-21611	NN	denotes	serum
T6032	21612-21616	JJ	denotes	free
T6033	21611-21612	HYPH	denotes	-
T6034	21617-21622	NNS	denotes	media
T6035	21623-21626	CC	denotes	and
T6036	21627-21633	VBN	denotes	placed
T6037	21634-21636	IN	denotes	on
T6038	21637-21640	DT	denotes	the
T6039	21641-21646	NNS	denotes	cells
T6040	21647-21649	TO	denotes	to
T6041	21650-21658	VB	denotes	incubate
T6042	21659-21662	IN	denotes	for
T6043	21663-21666	CD	denotes	two
T6044	21667-21672	NNS	denotes	hours
T6045	21672-21673	.	denotes	.
T6046	21673-21739	sentence	denotes	The cells were washed once with 1x PBS and fresh media was added.
T6047	21674-21677	DT	denotes	The
T6048	21678-21683	NNS	denotes	cells
T6049	21689-21695	VBN	denotes	washed
T6050	21684-21688	VBD	denotes	were
T6051	21696-21700	RB	denotes	once
T6052	21701-21705	IN	denotes	with
T6053	21706-21708	NN	denotes	1x
T6054	21709-21712	NNS	denotes	PBS
T6055	21713-21716	CC	denotes	and
T6056	21717-21722	JJ	denotes	fresh
T6057	21723-21728	NNS	denotes	media
T6058	21733-21738	VBN	denotes	added
T6059	21729-21732	VBD	denotes	was
T6060	21738-21739	.	denotes	.
T6061	21739-21878	sentence	denotes	The transfected cells were then incubated for 48 hours to ensure expression and subsequently fixed with a 1:1 mixture of acetone:methanol.
T6062	21740-21743	DT	denotes	The
T6063	21756-21761	NNS	denotes	cells
T6064	21744-21755	VBN	denotes	transfected
T6065	21772-21781	VBN	denotes	incubated
T6066	21762-21766	VBD	denotes	were
T6067	21767-21771	RB	denotes	then
T6068	21782-21785	IN	denotes	for
T6069	21786-21788	CD	denotes	48
T6070	21789-21794	NNS	denotes	hours
T6071	21795-21797	TO	denotes	to
T6072	21798-21804	VB	denotes	ensure
T6073	21805-21815	NN	denotes	expression
T6074	21816-21819	CC	denotes	and
T6075	21820-21832	RB	denotes	subsequently
T6076	21833-21838	VBN	denotes	fixed
T6077	21839-21843	IN	denotes	with
T6078	21844-21845	DT	denotes	a
T6079	21850-21857	NN	denotes	mixture
T6080	21846-21847	CD	denotes	1
T6081	21847-21848	SYM	denotes	:
T6082	21848-21849	CD	denotes	1
T6083	21858-21860	IN	denotes	of
T6084	21861-21868	NN	denotes	acetone
T6085	21868-21869	SYM	denotes	:
T6086	21869-21877	NN	denotes	methanol
T6087	21877-21878	.	denotes	.
T6088	21878-22168	sentence	denotes	In preparation for immunostaining, cells were blocked with 2% goat serum in PBS for 30 minutes, then incubated with 1:50 dilution of primary antibody anti-Abcg5 in 2% goat serum-PBS for 1.5 hours, and subsequently incubated with secondary rhodamine anti-rabbit IgG antibody for 30 minutes.
T6089	21879-21881	IN	denotes	In
T6090	21925-21932	VBN	denotes	blocked
T6091	21882-21893	NN	denotes	preparation
T6092	21894-21897	IN	denotes	for
T6093	21898-21912	NN	denotes	immunostaining
T6094	21912-21914	,	denotes	,
T6095	21914-21919	NNS	denotes	cells
T6096	21920-21924	VBD	denotes	were
T6097	21933-21937	IN	denotes	with
T6098	21938-21939	CD	denotes	2
T6099	21939-21940	NN	denotes	%
T6100	21946-21951	NN	denotes	serum
T6101	21941-21945	NN	denotes	goat
T6102	21952-21954	IN	denotes	in
T6103	21955-21958	NN	denotes	PBS
T6104	21959-21962	IN	denotes	for
T6105	21963-21965	CD	denotes	30
T6106	21966-21973	NNS	denotes	minutes
T6107	21973-21975	,	denotes	,
T6108	21975-21979	RB	denotes	then
T6109	21980-21989	VBN	denotes	incubated
T6110	21990-21994	IN	denotes	with
T6111	21995-21996	CD	denotes	1
T6112	22000-22008	NN	denotes	dilution
T6113	21996-21997	SYM	denotes	:
T6114	21997-21999	CD	denotes	50
T6115	22009-22011	IN	denotes	of
T6116	22012-22019	JJ	denotes	primary
T6117	22020-22028	NN	denotes	antibody
T6118	22029-22039	NN	denotes	anti-Abcg5
T6119	22040-22042	IN	denotes	in
T6120	22043-22044	CD	denotes	2
T6121	22044-22045	NN	denotes	%
T6122	22057-22060	NN	denotes	PBS
T6123	22046-22050	NN	denotes	goat
T6124	22051-22056	NN	denotes	serum
T6125	22056-22057	HYPH	denotes	-
T6126	22061-22064	IN	denotes	for
T6127	22065-22068	CD	denotes	1.5
T6128	22069-22074	NNS	denotes	hours
T6129	22074-22076	,	denotes	,
T6130	22076-22079	CC	denotes	and
T6131	22080-22092	RB	denotes	subsequently
T6132	22093-22102	VBN	denotes	incubated
T6133	22103-22107	IN	denotes	with
T6134	22108-22117	JJ	denotes	secondary
T6135	22144-22152	NN	denotes	antibody
T6136	22118-22127	NN	denotes	rhodamine
T6137	22128-22139	NN	denotes	anti-rabbit
T6138	22140-22143	NN	denotes	IgG
T6139	22153-22156	IN	denotes	for
T6140	22157-22159	CD	denotes	30
T6141	22160-22167	NNS	denotes	minutes
T6142	22167-22168	.	denotes	.
T6143	22168-22250	sentence	denotes	Cells were then observed under an Olympus BX-5 confocal microscope with Fluoview.
T6144	22169-22174	NNS	denotes	Cells
T6145	22185-22193	VBN	denotes	observed
T6146	22175-22179	VBD	denotes	were
T6147	22180-22184	RB	denotes	then
T6148	22194-22199	IN	denotes	under
T6149	22200-22202	DT	denotes	an
T6150	22225-22235	NN	denotes	microscope
T6151	22203-22210	NNP	denotes	Olympus
T6152	22211-22213	NN	denotes	BX
T6153	22213-22214	HYPH	denotes	-
T6154	22214-22215	CD	denotes	5
T6155	22216-22224	JJ	denotes	confocal
T6156	22236-22240	IN	denotes	with
T6157	22241-22249	NNP	denotes	Fluoview
T6158	22249-22250	.	denotes	.
T6247	22252-22259	JJ	denotes	Dynamic
T6248	22268-22273	NN	denotes	lipid
T6249	22260-22267	JJ	denotes	biliary
T6250	22274-22284	NN	denotes	collection
T6251	22285-22288	CC	denotes	and
T6252	22289-22297	NN	denotes	analysis
T6253	22297-22404	sentence	denotes	Mice were anaesthetized by intraperitoneal injection of 1 mL/kg fentanyl/fluanisone and 10 mg/kg Diazepam.
T6254	22298-22302	NNS	denotes	Mice
T6255	22308-22321	VBN	denotes	anaesthetized
T6256	22303-22307	VBD	denotes	were
T6257	22322-22324	IN	denotes	by
T6258	22325-22340	JJ	denotes	intraperitoneal
T6259	22341-22350	NN	denotes	injection
T6260	22351-22353	IN	denotes	of
T6261	22354-22355	CD	denotes	1
T6262	22356-22358	NN	denotes	mL
T6263	22371-22381	NN	denotes	fluanisone
T6264	22358-22359	SYM	denotes	/
T6265	22359-22361	NN	denotes	kg
T6266	22362-22370	NN	denotes	fentanyl
T6267	22370-22371	HYPH	denotes	/
T6268	22382-22385	CC	denotes	and
T6269	22386-22388	CD	denotes	10
T6270	22389-22391	NN	denotes	mg
T6271	22395-22403	NN	denotes	Diazepam
T6272	22391-22392	SYM	denotes	/
T6273	22392-22394	NN	denotes	kg
T6274	22403-22404	.	denotes	.
T6275	22404-22504	sentence	denotes	The abdomen was opened and, after distal ligation of the bile duct, the gallbladder was cannulated.
T6276	22405-22408	DT	denotes	The
T6277	22409-22416	NN	denotes	abdomen
T6278	22421-22427	VBN	denotes	opened
T6279	22417-22420	VBD	denotes	was
T6280	22428-22431	CC	denotes	and
T6281	22431-22433	,	denotes	,
T6282	22433-22438	IN	denotes	after
T6283	22493-22503	VBN	denotes	cannulated
T6284	22439-22445	JJ	denotes	distal
T6285	22446-22454	NN	denotes	ligation
T6286	22455-22457	IN	denotes	of
T6287	22458-22461	DT	denotes	the
T6288	22467-22471	NN	denotes	duct
T6289	22462-22466	NN	denotes	bile
T6290	22471-22473	,	denotes	,
T6291	22473-22476	DT	denotes	the
T6292	22477-22488	NN	denotes	gallbladder
T6293	22489-22492	VBD	denotes	was
T6294	22503-22504	.	denotes	.
T6295	22504-22583	sentence	denotes	Bile sampling started directly after cannulation and was collected for 10 min.
T6296	22505-22509	NN	denotes	Bile
T6297	22510-22518	NN	denotes	sampling
T6298	22519-22526	VBD	denotes	started
T6299	22527-22535	RB	denotes	directly
T6300	22536-22541	IN	denotes	after
T6301	22542-22553	NN	denotes	cannulation
T6302	22554-22557	CC	denotes	and
T6303	22558-22561	VBD	denotes	was
T6304	22562-22571	VBN	denotes	collected
T6305	22572-22575	IN	denotes	for
T6306	22576-22578	CD	denotes	10
T6307	22579-22582	NN	denotes	min
T6308	22582-22583	.	denotes	.
T6309	22583-22625	sentence	denotes	Bile flow was determined gravimetrically.
T6310	22584-22588	NN	denotes	Bile
T6311	22589-22593	NN	denotes	flow
T6312	22598-22608	VBN	denotes	determined
T6313	22594-22597	VBD	denotes	was
T6314	22609-22624	RB	denotes	gravimetrically
T6315	22624-22625	.	denotes	.
T6316	22625-22729	sentence	denotes	Biliary bile salt, sterol and phospholipid concentrations were determined as described previously [32].
T6317	22626-22633	JJ	denotes	Biliary
T6318	22634-22638	NN	denotes	bile
T6319	22669-22683	NNS	denotes	concentrations
T6320	22639-22643	NN	denotes	salt
T6321	22643-22645	,	denotes	,
T6322	22645-22651	NN	denotes	sterol
T6323	22652-22655	CC	denotes	and
T6324	22656-22668	NN	denotes	phospholipid
T6325	22689-22699	VBN	denotes	determined
T6326	22684-22688	VBD	denotes	were
T6327	22700-22702	IN	denotes	as
T6328	22703-22712	VBN	denotes	described
T6329	22713-22723	RB	denotes	previously
T6330	22724-22725	-LRB-	denotes	[
T6331	22725-22727	CD	denotes	32
T6332	22727-22728	-RRB-	denotes	]
T6333	22728-22729	.	denotes	.
T6334	22729-22865	sentence	denotes	To determine maximal secretion rates of biliary lipid secretion, bile was diverted for 90 min to deplete the endogenous bile salt pool.
T6335	22730-22732	TO	denotes	To
T6336	22733-22742	VB	denotes	determine
T6337	22804-22812	VBN	denotes	diverted
T6338	22743-22750	JJ	denotes	maximal
T6339	22761-22766	NNS	denotes	rates
T6340	22751-22760	NN	denotes	secretion
T6341	22767-22769	IN	denotes	of
T6342	22770-22777	JJ	denotes	biliary
T6343	22778-22783	NN	denotes	lipid
T6344	22784-22793	NN	denotes	secretion
T6345	22793-22795	,	denotes	,
T6346	22795-22799	NN	denotes	bile
T6347	22800-22803	VBD	denotes	was
T6348	22813-22816	IN	denotes	for
T6349	22817-22819	CD	denotes	90
T6350	22820-22823	NN	denotes	min
T6351	22824-22826	TO	denotes	to
T6352	22827-22834	VB	denotes	deplete
T6353	22835-22838	DT	denotes	the
T6354	22860-22864	NN	denotes	pool
T6355	22839-22849	JJ	denotes	endogenous
T6356	22850-22854	NN	denotes	bile
T6357	22855-22859	NN	denotes	salt
T6358	22864-22865	.	denotes	.
T6359	22865-23054	sentence	denotes	Subsequently, tauroursodeoxycholate (Sigma Chemical Co., MO, USA) dissolved in PBS was infused into the jugular vein in stepwise increasing rates from 600–1,800 nmol/min/100 g body weight.
T6360	22866-22878	RB	denotes	Subsequently
T6361	22953-22960	VBN	denotes	infused
T6362	22878-22880	,	denotes	,
T6363	22880-22901	NN	denotes	tauroursodeoxycholate
T6364	22902-22903	-LRB-	denotes	(
T6365	22918-22921	NNP	denotes	Co.
T6366	22903-22908	NNP	denotes	Sigma
T6367	22909-22917	NNP	denotes	Chemical
T6368	22921-22923	,	denotes	,
T6369	22923-22925	NNP	denotes	MO
T6370	22925-22927	,	denotes	,
T6371	22927-22930	NNP	denotes	USA
T6372	22930-22931	-RRB-	denotes	)
T6373	22932-22941	VBN	denotes	dissolved
T6374	22942-22944	IN	denotes	in
T6375	22945-22948	NN	denotes	PBS
T6376	22949-22952	VBD	denotes	was
T6377	22961-22965	IN	denotes	into
T6378	22966-22969	DT	denotes	the
T6379	22978-22982	NN	denotes	vein
T6380	22970-22977	JJ	denotes	jugular
T6381	22983-22985	IN	denotes	in
T6382	22986-22994	JJ	denotes	stepwise
T6383	23006-23011	NNS	denotes	rates
T6384	22995-23005	VBG	denotes	increasing
T6385	23012-23016	IN	denotes	from
T6386	23017-23020	CD	denotes	600
T6387	23021-23026	CD	denotes	1,800
T6388	23020-23021	SYM	denotes	–
T6389	23027-23031	NN	denotes	nmol
T6390	23031-23032	SYM	denotes	/
T6391	23032-23035	NN	denotes	min
T6392	23035-23036	SYM	denotes	/
T6393	23047-23053	NN	denotes	weight
T6394	23036-23039	CD	denotes	100
T6395	23040-23041	NN	denotes	g
T6396	23042-23046	NN	denotes	body
T6397	23053-23054	.	denotes	.
T6398	23054-23139	sentence	denotes	Bile was collected in 10 min fractions and analyzed for bile salt and lipid content.
T6399	23055-23059	NN	denotes	Bile
T6400	23064-23073	VBN	denotes	collected
T6401	23060-23063	VBD	denotes	was
T6402	23074-23076	IN	denotes	in
T6403	23077-23079	CD	denotes	10
T6404	23080-23083	NN	denotes	min
T6405	23084-23093	NNS	denotes	fractions
T6406	23094-23097	CC	denotes	and
T6407	23098-23106	VBN	denotes	analyzed
T6408	23107-23110	IN	denotes	for
T6409	23111-23115	NN	denotes	bile
T6410	23131-23138	NN	denotes	content
T6411	23116-23120	NN	denotes	salt
T6412	23121-23124	CC	denotes	and
T6413	23125-23130	NN	denotes	lipid
T6414	23138-23139	.	denotes	.
T6415	23139-23314	sentence	denotes	In separate experiments, following distal ligation of the bile duct and cannulation of gallbladder, bile was diverted for 30 minutes while mice were infused with sterile PBS.
T6416	23140-23142	IN	denotes	In
T6417	23249-23257	VBN	denotes	diverted
T6418	23143-23151	JJ	denotes	separate
T6419	23152-23163	NNS	denotes	experiments
T6420	23163-23165	,	denotes	,
T6421	23165-23174	VBG	denotes	following
T6422	23175-23181	JJ	denotes	distal
T6423	23182-23190	NN	denotes	ligation
T6424	23191-23193	IN	denotes	of
T6425	23194-23197	DT	denotes	the
T6426	23203-23207	NN	denotes	duct
T6427	23198-23202	NN	denotes	bile
T6428	23208-23211	CC	denotes	and
T6429	23212-23223	NN	denotes	cannulation
T6430	23224-23226	IN	denotes	of
T6431	23227-23238	NN	denotes	gallbladder
T6432	23238-23240	,	denotes	,
T6433	23240-23244	NN	denotes	bile
T6434	23245-23248	VBD	denotes	was
T6435	23258-23261	IN	denotes	for
T6436	23262-23264	CD	denotes	30
T6437	23265-23272	NNS	denotes	minutes
T6438	23273-23278	IN	denotes	while
T6439	23289-23296	VBN	denotes	infused
T6440	23279-23283	NNS	denotes	mice
T6441	23284-23288	VBD	denotes	were
T6442	23297-23301	IN	denotes	with
T6443	23302-23309	JJ	denotes	sterile
T6444	23310-23313	NN	denotes	PBS
T6445	23313-23314	.	denotes	.
T6446	23314-23470	sentence	denotes	Subsequently, the mice were infused continuously with 1,200 nmol/min/100 g body weight of tauroursodeoxycholate and two 30-minute fractions were collected.
T6447	23315-23327	RB	denotes	Subsequently
T6448	23343-23350	VBN	denotes	infused
T6449	23327-23329	,	denotes	,
T6450	23329-23332	DT	denotes	the
T6451	23333-23337	NNS	denotes	mice
T6452	23338-23342	VBD	denotes	were
T6453	23351-23363	RB	denotes	continuously
T6454	23364-23368	IN	denotes	with
T6455	23369-23374	CD	denotes	1,200
T6456	23375-23379	NN	denotes	nmol
T6457	23379-23380	SYM	denotes	/
T6458	23380-23383	NN	denotes	min
T6459	23383-23384	SYM	denotes	/
T6460	23395-23401	NN	denotes	weight
T6461	23384-23387	CD	denotes	100
T6462	23388-23389	NN	denotes	g
T6463	23390-23394	NN	denotes	body
T6464	23402-23404	IN	denotes	of
T6465	23405-23426	NN	denotes	tauroursodeoxycholate
T6466	23427-23430	CC	denotes	and
T6467	23431-23434	CD	denotes	two
T6468	23445-23454	NNS	denotes	fractions
T6469	23435-23437	CD	denotes	30
T6470	23438-23444	NN	denotes	minute
T6471	23437-23438	HYPH	denotes	-
T6472	23460-23469	VBN	denotes	collected
T6473	23455-23459	VBD	denotes	were
T6474	23469-23470	.	denotes	.
T6475	23470-23543	sentence	denotes	Sterol analyses of bile was performed by GC analysis as described above.
T6476	23471-23477	NN	denotes	Sterol
T6477	23478-23486	NNS	denotes	analyses
T6478	23499-23508	VBN	denotes	performed
T6479	23487-23489	IN	denotes	of
T6480	23490-23494	NN	denotes	bile
T6481	23495-23498	VBD	denotes	was
T6482	23509-23511	IN	denotes	by
T6483	23512-23514	NN	denotes	GC
T6484	23515-23523	NN	denotes	analysis
T6485	23524-23526	IN	denotes	as
T6486	23527-23536	VBN	denotes	described
T6487	23537-23542	RB	denotes	above
T6488	23542-23543	.	denotes	.
T6686	23545-23550	NN	denotes	Assay
T6687	23551-23553	IN	denotes	of
T6688	23554-23557	NN	denotes	HMG
T6689	23558-23561	NN	denotes	CoA
T6690	23557-23558	HYPH	denotes	-
T6691	23562-23565	CC	denotes	and
T6692	23566-23577	NN	denotes	cholesterol
T6693	23593-23599	NN	denotes	enzyme
T6694	23578-23579	CD	denotes	7
T6695	23579-23580	NN	denotes	α
T6696	23581-23592	NN	denotes	hydroxylase
T6697	23580-23581	HYPH	denotes	-
T6698	23600-23608	NN	denotes	activity
T6699	23608-23682	sentence	denotes	Mouse liver microsomes were prepared by differential centrifugation [33].
T6700	23609-23614	NN	denotes	Mouse
T6701	23615-23620	NN	denotes	liver
T6702	23621-23631	NNS	denotes	microsomes
T6703	23637-23645	VBN	denotes	prepared
T6704	23632-23636	VBD	denotes	were
T6705	23646-23648	IN	denotes	by
T6706	23649-23661	JJ	denotes	differential
T6707	23662-23676	NN	denotes	centrifugation
T6708	23677-23678	-LRB-	denotes	[
T6709	23678-23680	CD	denotes	33
T6710	23680-23681	-RRB-	denotes	]
T6711	23681-23682	.	denotes	.
T6712	23682-23855	sentence	denotes	Briefly, 0.1–0.2 g liver was homogenized in a Potter-Elvjhem homogenizer with five volumes of buffer (0.25 M sucrose, 0.1 M Tris, 0.1 mM disodium EDTA, 0.1 mM DTT, pH 7.4).
T6713	23683-23690	RB	denotes	Briefly
T6714	23712-23723	VBN	denotes	homogenized
T6715	23690-23692	,	denotes	,
T6716	23692-23695	CD	denotes	0.1
T6717	23696-23699	CD	denotes	0.2
T6718	23695-23696	SYM	denotes	–
T6719	23700-23701	NN	denotes	g
T6720	23702-23707	NN	denotes	liver
T6721	23708-23711	VBD	denotes	was
T6722	23724-23726	IN	denotes	in
T6723	23727-23728	DT	denotes	a
T6724	23744-23755	NN	denotes	homogenizer
T6725	23729-23735	NNP	denotes	Potter
T6726	23736-23743	NNP	denotes	Elvjhem
T6727	23735-23736	HYPH	denotes	-
T6728	23756-23760	IN	denotes	with
T6729	23761-23765	CD	denotes	five
T6730	23766-23773	NNS	denotes	volumes
T6731	23774-23776	IN	denotes	of
T6732	23777-23783	NN	denotes	buffer
T6733	23784-23785	-LRB-	denotes	(
T6734	23785-23789	CD	denotes	0.25
T6735	23790-23791	NN	denotes	M
T6736	23792-23799	NN	denotes	sucrose
T6737	23799-23801	,	denotes	,
T6738	23801-23804	CD	denotes	0.1
T6739	23805-23806	NN	denotes	M
T6740	23807-23811	NN	denotes	Tris
T6741	23811-23813	,	denotes	,
T6742	23813-23816	CD	denotes	0.1
T6743	23817-23819	NN	denotes	mM
T6744	23829-23833	NN	denotes	EDTA
T6745	23820-23828	NN	denotes	disodium
T6746	23833-23835	,	denotes	,
T6747	23835-23838	CD	denotes	0.1
T6748	23839-23841	NN	denotes	mM
T6749	23842-23845	NN	denotes	DTT
T6750	23845-23847	,	denotes	,
T6751	23847-23849	NN	denotes	pH
T6752	23850-23853	CD	denotes	7.4
T6753	23853-23854	-RRB-	denotes	)
T6754	23854-23855	.	denotes	.
T6755	23855-23936	sentence	denotes	Microsomes were isolated by differential centrifugation (10,000 g to 100,000 g).
T6756	23856-23866	NNS	denotes	Microsomes
T6757	23872-23880	VBN	denotes	isolated
T6758	23867-23871	VBD	denotes	were
T6759	23881-23883	IN	denotes	by
T6760	23884-23896	JJ	denotes	differential
T6761	23897-23911	NN	denotes	centrifugation
T6762	23912-23913	-LRB-	denotes	(
T6763	23920-23921	NN	denotes	g
T6764	23913-23919	CD	denotes	10,000
T6765	23922-23924	IN	denotes	to
T6766	23925-23932	CD	denotes	100,000
T6767	23933-23934	NN	denotes	g
T6768	23934-23935	-RRB-	denotes	)
T6769	23935-23936	.	denotes	.
T6770	23936-24112	sentence	denotes	The pellets were washed and resuspended in storage buffer (0.1 M dipotassium phosphate, 0.05 M KCl, 1 mM DTT, 5 mM disodium EDTA, 20% glycerol, pH 7.4) at 25–30 mg protein/ml.
T6771	23937-23940	DT	denotes	The
T6772	23941-23948	NNS	denotes	pellets
T6773	23954-23960	VBN	denotes	washed
T6774	23949-23953	VBD	denotes	were
T6775	23961-23964	CC	denotes	and
T6776	23965-23976	VBN	denotes	resuspended
T6777	23977-23979	IN	denotes	in
T6778	23980-23987	NN	denotes	storage
T6779	23988-23994	NN	denotes	buffer
T6780	23995-23996	-LRB-	denotes	(
T6781	24014-24023	NN	denotes	phosphate
T6782	23996-23999	CD	denotes	0.1
T6783	24000-24001	NN	denotes	M
T6784	24002-24013	NN	denotes	dipotassium
T6785	24023-24025	,	denotes	,
T6786	24025-24029	CD	denotes	0.05
T6787	24030-24031	NN	denotes	M
T6788	24032-24035	NN	denotes	KCl
T6789	24035-24037	,	denotes	,
T6790	24037-24038	CD	denotes	1
T6791	24039-24041	NN	denotes	mM
T6792	24042-24045	NN	denotes	DTT
T6793	24045-24047	,	denotes	,
T6794	24047-24048	CD	denotes	5
T6795	24049-24051	NN	denotes	mM
T6796	24061-24065	NN	denotes	EDTA
T6797	24052-24060	NN	denotes	disodium
T6798	24065-24067	,	denotes	,
T6799	24067-24069	CD	denotes	20
T6800	24069-24070	NN	denotes	%
T6801	24071-24079	NN	denotes	glycerol
T6802	24079-24081	,	denotes	,
T6803	24081-24083	NN	denotes	pH
T6804	24084-24087	CD	denotes	7.4
T6805	24087-24088	-RRB-	denotes	)
T6806	24089-24091	IN	denotes	at
T6807	24092-24094	CD	denotes	25
T6808	24095-24097	CD	denotes	30
T6809	24094-24095	SYM	denotes	–
T6810	24098-24100	NN	denotes	mg
T6811	24101-24108	NN	denotes	protein
T6812	24108-24109	SYM	denotes	/
T6813	24109-24111	NN	denotes	ml
T6814	24111-24112	.	denotes	.
T6815	24112-24197	sentence	denotes	Protein concentrations were determined according to the method of Lowry et al. [34].
T6816	24113-24120	NN	denotes	Protein
T6817	24121-24135	NNS	denotes	concentrations
T6818	24141-24151	VBN	denotes	determined
T6819	24136-24140	VBD	denotes	were
T6820	24152-24161	VBG	denotes	according
T6821	24162-24164	IN	denotes	to
T6822	24165-24168	DT	denotes	the
T6823	24169-24175	NN	denotes	method
T6824	24176-24178	IN	denotes	of
T6825	24179-24184	NNP	denotes	Lowry
T6826	24185-24187	FW	denotes	et
T6827	24188-24191	FW	denotes	al.
T6828	24192-24193	-LRB-	denotes	[
T6829	24193-24195	CD	denotes	34
T6830	24195-24196	-RRB-	denotes	]
T6831	24196-24197	.	denotes	.
T6832	24197-24361	sentence	denotes	The activity of cholesterol 7α-hydroxylase (EC 1.14.12.17) was measured by an isotope incorporation method according to Shefer et al. [33] with some modifications.
T6833	24198-24201	DT	denotes	The
T6834	24202-24210	NN	denotes	activity
T6835	24261-24269	VBN	denotes	measured
T6836	24211-24213	IN	denotes	of
T6837	24214-24225	NN	denotes	cholesterol
T6838	24229-24240	NN	denotes	hydroxylase
T6839	24226-24227	CD	denotes	7
T6840	24227-24228	NN	denotes	α
T6841	24228-24229	HYPH	denotes	-
T6842	24241-24242	-LRB-	denotes	(
T6843	24242-24244	NN	denotes	EC
T6844	24245-24255	CD	denotes	1.14.12.17
T6845	24255-24256	-RRB-	denotes	)
T6846	24257-24260	VBD	denotes	was
T6847	24270-24272	IN	denotes	by
T6848	24273-24275	DT	denotes	an
T6849	24298-24304	NN	denotes	method
T6850	24276-24283	NN	denotes	isotope
T6851	24284-24297	NN	denotes	incorporation
T6852	24305-24314	VBG	denotes	according
T6853	24315-24317	IN	denotes	to
T6854	24318-24324	NNP	denotes	Shefer
T6855	24325-24327	FW	denotes	et
T6856	24328-24331	FW	denotes	al.
T6857	24332-24333	-LRB-	denotes	[
T6858	24333-24335	CD	denotes	33
T6859	24335-24336	-RRB-	denotes	]
T6860	24337-24341	IN	denotes	with
T6861	24342-24346	DT	denotes	some
T6862	24347-24360	NNS	denotes	modifications
T6863	24360-24361	.	denotes	.
T6864	24361-24661	sentence	denotes	The reaction mixture (final volume 0.5 ml) consisted of potassium phosphate buffer (100 mM K2HPO4, 0.1 mM EDTA, 5 mM DTT, 30 mM nicotinamide, pH 7.4), [14C]-cholesterol (5 × 105 dpm) solubilized in 50 μl of 25% (wt/vol) β-cyclodextrin (final concentration 0.8%), and 50–200 μg of microsomal protein.
T6865	24362-24365	DT	denotes	The
T6866	24375-24382	NN	denotes	mixture
T6867	24366-24374	NN	denotes	reaction
T6868	24405-24414	VBD	denotes	consisted
T6869	24383-24384	-LRB-	denotes	(
T6870	24401-24403	NN	denotes	ml
T6871	24384-24389	JJ	denotes	final
T6872	24390-24396	NN	denotes	volume
T6873	24397-24400	CD	denotes	0.5
T6874	24403-24404	-RRB-	denotes	)
T6875	24415-24417	IN	denotes	of
T6876	24418-24427	NN	denotes	potassium
T6877	24428-24437	NN	denotes	phosphate
T6878	24438-24444	NN	denotes	buffer
T6879	24445-24446	-LRB-	denotes	(
T6880	24453-24459	NN	denotes	K2HPO4
T6881	24446-24449	CD	denotes	100
T6882	24450-24452	NN	denotes	mM
T6883	24459-24461	,	denotes	,
T6884	24461-24464	CD	denotes	0.1
T6885	24465-24467	NN	denotes	mM
T6886	24468-24472	NN	denotes	EDTA
T6887	24472-24474	,	denotes	,
T6888	24474-24475	CD	denotes	5
T6889	24476-24478	NN	denotes	mM
T6890	24479-24482	NN	denotes	DTT
T6891	24482-24484	,	denotes	,
T6892	24484-24486	CD	denotes	30
T6893	24487-24489	NN	denotes	mM
T6894	24490-24502	NN	denotes	nicotinamide
T6895	24502-24504	,	denotes	,
T6896	24504-24506	NN	denotes	pH
T6897	24507-24510	CD	denotes	7.4
T6898	24510-24511	-RRB-	denotes	)
T6899	24511-24513	,	denotes	,
T6900	24513-24514	-LRB-	denotes	[
T6901	24514-24517	NN	denotes	14C
T6902	24517-24518	-RRB-	denotes	]
T6903	24518-24519	HYPH	denotes	-
T6904	24519-24530	NN	denotes	cholesterol
T6905	24531-24532	-LRB-	denotes	(
T6906	24540-24543	NN	denotes	dpm
T6907	24532-24533	CD	denotes	5
T6908	24536-24539	CD	denotes	105
T6909	24534-24535	SYM	denotes	×
T6910	24543-24544	-RRB-	denotes	)
T6911	24545-24556	VBN	denotes	solubilized
T6912	24557-24559	IN	denotes	in
T6913	24560-24562	CD	denotes	50
T6914	24563-24565	NN	denotes	μl
T6915	24566-24568	IN	denotes	of
T6916	24569-24571	CD	denotes	25
T6917	24571-24572	NN	denotes	%
T6918	24584-24596	NN	denotes	cyclodextrin
T6919	24573-24574	-LRB-	denotes	(
T6920	24577-24580	NN	denotes	vol
T6921	24574-24576	NN	denotes	wt
T6922	24576-24577	HYPH	denotes	/
T6923	24580-24581	-RRB-	denotes	)
T6924	24582-24583	NN	denotes	β
T6925	24583-24584	HYPH	denotes	-
T6926	24597-24598	-LRB-	denotes	(
T6927	24621-24622	NN	denotes	%
T6928	24598-24603	JJ	denotes	final
T6929	24604-24617	NN	denotes	concentration
T6930	24618-24621	CD	denotes	0.8
T6931	24622-24623	-RRB-	denotes	)
T6932	24623-24625	,	denotes	,
T6933	24625-24628	CC	denotes	and
T6934	24629-24631	CD	denotes	50
T6935	24632-24635	CD	denotes	200
T6936	24631-24632	SYM	denotes	–
T6937	24636-24638	NN	denotes	μg
T6938	24639-24641	IN	denotes	of
T6939	24642-24652	JJ	denotes	microsomal
T6940	24653-24660	NN	denotes	protein
T6941	24660-24661	.	denotes	.
T6942	24661-24863	sentence	denotes	The reaction was initiated by the addition of NADPH or an NADPH-generating system (3.4 mM NADP+, 30 mM glucose-6-phosphate, 0.3 U of glucose-6-phosphate dehydrogenase) and continued for 15 min at 37°C.
T6943	24662-24665	DT	denotes	The
T6944	24666-24674	NN	denotes	reaction
T6945	24679-24688	VBN	denotes	initiated
T6946	24675-24678	VBD	denotes	was
T6947	24689-24691	IN	denotes	by
T6948	24692-24695	DT	denotes	the
T6949	24696-24704	NN	denotes	addition
T6950	24705-24707	IN	denotes	of
T6951	24708-24713	NN	denotes	NADPH
T6952	24714-24716	CC	denotes	or
T6953	24717-24719	DT	denotes	an
T6954	24737-24743	NN	denotes	system
T6955	24720-24725	NN	denotes	NADPH
T6956	24726-24736	VBG	denotes	generating
T6957	24725-24726	HYPH	denotes	-
T6958	24744-24745	-LRB-	denotes	(
T6959	24745-24748	CD	denotes	3.4
T6960	24749-24751	NN	denotes	mM
T6961	24752-24757	NN	denotes	NADP+
T6962	24757-24759	,	denotes	,
T6963	24759-24761	CD	denotes	30
T6964	24762-24764	NN	denotes	mM
T6965	24775-24784	NN	denotes	phosphate
T6966	24765-24772	NN	denotes	glucose
T6967	24772-24773	HYPH	denotes	-
T6968	24773-24774	CD	denotes	6
T6969	24774-24775	HYPH	denotes	-
T6970	24784-24786	,	denotes	,
T6971	24786-24789	CD	denotes	0.3
T6972	24790-24791	NN	denotes	U
T6973	24792-24794	IN	denotes	of
T6974	24795-24802	NN	denotes	glucose
T6975	24805-24814	NN	denotes	phosphate
T6976	24802-24803	HYPH	denotes	-
T6977	24803-24804	CD	denotes	6
T6978	24804-24805	HYPH	denotes	-
T6979	24815-24828	NN	denotes	dehydrogenase
T6980	24828-24829	-RRB-	denotes	)
T6981	24830-24833	CC	denotes	and
T6982	24834-24843	VBN	denotes	continued
T6983	24844-24847	IN	denotes	for
T6984	24848-24850	CD	denotes	15
T6985	24851-24854	NN	denotes	min
T6986	24855-24857	IN	denotes	at
T6987	24858-24860	CD	denotes	37
T6988	24860-24862	NN	denotes	°C
T6989	24862-24863	.	denotes	.
T6990	24863-24990	sentence	denotes	The reaction was stopped with 0.5 ml of 1 N KOH, 5 μg of butylated hydroxytoluene, and 10 μl of ethanolic potassium hydroxide.
T6991	24864-24867	DT	denotes	The
T6992	24868-24876	NN	denotes	reaction
T6993	24881-24888	VBN	denotes	stopped
T6994	24877-24880	VBD	denotes	was
T6995	24889-24893	IN	denotes	with
T6996	24894-24897	CD	denotes	0.5
T6997	24898-24900	NN	denotes	ml
T6998	24901-24903	IN	denotes	of
T6999	24904-24905	CD	denotes	1
T7000	24906-24907	NN	denotes	N
T7001	24908-24911	NN	denotes	KOH
T7002	24911-24913	,	denotes	,
T7003	24913-24914	CD	denotes	5
T7004	24915-24917	NN	denotes	μg
T7005	24918-24920	IN	denotes	of
T7006	24921-24930	VBN	denotes	butylated
T7007	24931-24945	NN	denotes	hydroxytoluene
T7008	24945-24947	,	denotes	,
T7009	24947-24950	CC	denotes	and
T7010	24951-24953	CD	denotes	10
T7011	24954-24956	NN	denotes	μl
T7012	24957-24959	IN	denotes	of
T7013	24960-24969	JJ	denotes	ethanolic
T7014	24980-24989	NN	denotes	hydroxide
T7015	24970-24979	NN	denotes	potassium
T7016	24989-24990	.	denotes	.
T7017	24990-25080	sentence	denotes	[3H] 7α-hydroxycholesterol (1 × 104 dpm/5 μg) was added as an internal recovery standard.
T7018	24991-24992	-LRB-	denotes	[
T7019	24992-24994	NN	denotes	3H
T7020	25041-25046	VBN	denotes	added
T7021	24994-24995	-RRB-	denotes	]
T7022	24996-24997	CD	denotes	7
T7023	24997-24998	NN	denotes	α
T7024	24999-25017	NN	denotes	hydroxycholesterol
T7025	24998-24999	HYPH	denotes	-
T7026	25018-25019	-LRB-	denotes	(
T7027	25033-25035	NN	denotes	μg
T7028	25019-25020	CD	denotes	1
T7029	25023-25026	CD	denotes	104
T7030	25021-25022	SYM	denotes	×
T7031	25027-25030	NN	denotes	dpm
T7032	25030-25031	HYPH	denotes	/
T7033	25031-25032	CD	denotes	5
T7034	25035-25036	-RRB-	denotes	)
T7035	25037-25040	VBD	denotes	was
T7036	25047-25049	IN	denotes	as
T7037	25050-25052	DT	denotes	an
T7038	25071-25079	NN	denotes	standard
T7039	25053-25061	JJ	denotes	internal
T7040	25062-25070	NN	denotes	recovery
T7041	25079-25080	.	denotes	.
T7042	25080-25234	sentence	denotes	After saponification at 37°C for one hour, sterols were extracted twice with 3 ml of n-hexane and the extracts were evaporated to dryness under nitrogen.
T7043	25081-25086	IN	denotes	After
T7044	25137-25146	VBN	denotes	extracted
T7045	25087-25101	NN	denotes	saponification
T7046	25102-25104	IN	denotes	at
T7047	25105-25107	CD	denotes	37
T7048	25107-25109	NN	denotes	°C
T7049	25110-25113	IN	denotes	for
T7050	25114-25117	CD	denotes	one
T7051	25118-25122	NN	denotes	hour
T7052	25122-25124	,	denotes	,
T7053	25124-25131	NNS	denotes	sterols
T7054	25132-25136	VBD	denotes	were
T7055	25147-25152	RB	denotes	twice
T7056	25153-25157	IN	denotes	with
T7057	25158-25159	CD	denotes	3
T7058	25160-25162	NN	denotes	ml
T7059	25163-25165	IN	denotes	of
T7060	25166-25167	NN	denotes	n
T7061	25168-25174	NN	denotes	hexane
T7062	25167-25168	HYPH	denotes	-
T7063	25175-25178	CC	denotes	and
T7064	25179-25182	DT	denotes	the
T7065	25183-25191	NNS	denotes	extracts
T7066	25197-25207	VBN	denotes	evaporated
T7067	25192-25196	VBD	denotes	were
T7068	25208-25210	IN	denotes	to
T7069	25211-25218	NN	denotes	dryness
T7070	25219-25224	IN	denotes	under
T7071	25225-25233	NN	denotes	nitrogen
T7072	25233-25234	.	denotes	.
T7073	25234-25405	sentence	denotes	The residue was dissolved in 0.3 ml of n-hexane:2-propanol (97.3 vol/vol) and applied to a silica column (500 mg; sep-pak, by 4 ml of n-hexane:2-propanol (97.3 vol/vol)).
T7074	25235-25238	DT	denotes	The
T7075	25239-25246	NN	denotes	residue
T7076	25251-25260	VBN	denotes	dissolved
T7077	25247-25250	VBD	denotes	was
T7078	25261-25263	IN	denotes	in
T7079	25264-25267	CD	denotes	0.3
T7080	25268-25270	NN	denotes	ml
T7081	25271-25273	IN	denotes	of
T7082	25274-25275	NN	denotes	n
T7083	25276-25282	NN	denotes	hexane
T7084	25275-25276	HYPH	denotes	-
T7085	25282-25283	:	denotes	:
T7086	25283-25284	CD	denotes	2
T7087	25285-25293	NN	denotes	propanol
T7088	25284-25285	HYPH	denotes	-
T7089	25294-25295	-LRB-	denotes	(
T7090	25304-25307	NN	denotes	vol
T7091	25295-25299	CD	denotes	97.3
T7092	25300-25303	NN	denotes	vol
T7093	25303-25304	HYPH	denotes	/
T7094	25307-25308	-RRB-	denotes	)
T7095	25309-25312	CC	denotes	and
T7096	25313-25320	VBN	denotes	applied
T7097	25321-25323	IN	denotes	to
T7098	25324-25325	DT	denotes	a
T7099	25333-25339	NN	denotes	column
T7100	25326-25332	NN	denotes	silica
T7101	25340-25341	-LRB-	denotes	(
T7102	25353-25356	NN	denotes	pak
T7103	25341-25344	CD	denotes	500
T7104	25345-25347	NN	denotes	mg
T7105	25347-25348	:	denotes	;
T7106	25349-25352	NN	denotes	sep
T7107	25352-25353	HYPH	denotes	-
T7108	25356-25358	,	denotes	,
T7109	25358-25360	IN	denotes	by
T7110	25361-25362	CD	denotes	4
T7111	25363-25365	NN	denotes	ml
T7112	25366-25368	IN	denotes	of
T7113	25369-25370	NN	denotes	n
T7114	25371-25377	NN	denotes	hexane
T7115	25370-25371	HYPH	denotes	-
T7116	25377-25378	:	denotes	:
T7117	25378-25379	CD	denotes	2
T7118	25380-25388	NN	denotes	propanol
T7119	25379-25380	HYPH	denotes	-
T7120	25389-25390	-LRB-	denotes	(
T7121	25399-25402	NN	denotes	vol
T7122	25390-25394	CD	denotes	97.3
T7123	25395-25398	NN	denotes	vol
T7124	25398-25399	HYPH	denotes	/
T7125	25402-25403	-RRB-	denotes	)
T7126	25403-25404	-RRB-	denotes	)
T7127	25404-25405	.	denotes	.
T7128	25405-25705	sentence	denotes	7α-hydroxycholesterol was eluted with 3 ml of n-hexane:2-propanol (80:20 vol:vol) and further isolated by TLC on silica gel plates (Silica gel 60, EM Science, Gibbestown, NJ, USA) with diethyl ether, and quantified by liquid scintillation counting using Ecolume (ICN Radiochemicals Irvine, CA, USA).
T7129	25406-25407	CD	denotes	7
T7130	25407-25408	NN	denotes	α
T7131	25409-25427	NN	denotes	hydroxycholesterol
T7132	25408-25409	HYPH	denotes	-
T7133	25432-25438	VBN	denotes	eluted
T7134	25428-25431	VBD	denotes	was
T7135	25439-25443	IN	denotes	with
T7136	25444-25445	CD	denotes	3
T7137	25446-25448	NN	denotes	ml
T7138	25449-25451	IN	denotes	of
T7139	25452-25453	NN	denotes	n
T7140	25454-25460	NN	denotes	hexane
T7141	25453-25454	HYPH	denotes	-
T7142	25460-25461	:	denotes	:
T7143	25461-25462	CD	denotes	2
T7144	25463-25471	NN	denotes	propanol
T7145	25462-25463	HYPH	denotes	-
T7146	25472-25473	-LRB-	denotes	(
T7147	25479-25482	NN	denotes	vol
T7148	25473-25475	CD	denotes	80
T7149	25475-25476	SYM	denotes	:
T7150	25476-25478	CD	denotes	20
T7151	25482-25483	SYM	denotes	:
T7152	25483-25486	NN	denotes	vol
T7153	25486-25487	-RRB-	denotes	)
T7154	25488-25491	CC	denotes	and
T7155	25492-25499	RB	denotes	further
T7156	25500-25508	VBN	denotes	isolated
T7157	25509-25511	IN	denotes	by
T7158	25512-25515	NN	denotes	TLC
T7159	25516-25518	IN	denotes	on
T7160	25519-25525	NN	denotes	silica
T7161	25526-25529	NN	denotes	gel
T7162	25530-25536	NNS	denotes	plates
T7163	25537-25538	-LRB-	denotes	(
T7164	25556-25563	NNP	denotes	Science
T7165	25538-25544	NN	denotes	Silica
T7166	25545-25548	NN	denotes	gel
T7167	25549-25551	CD	denotes	60
T7168	25551-25553	,	denotes	,
T7169	25553-25555	NNP	denotes	EM
T7170	25563-25565	,	denotes	,
T7171	25565-25575	NNP	denotes	Gibbestown
T7172	25575-25577	,	denotes	,
T7173	25577-25579	NNP	denotes	NJ
T7174	25579-25581	,	denotes	,
T7175	25581-25584	NNP	denotes	USA
T7176	25584-25585	-RRB-	denotes	)
T7177	25586-25590	IN	denotes	with
T7178	25591-25598	NN	denotes	diethyl
T7179	25599-25604	NN	denotes	ether
T7180	25604-25606	,	denotes	,
T7181	25606-25609	CC	denotes	and
T7182	25610-25620	VBN	denotes	quantified
T7183	25621-25623	IN	denotes	by
T7184	25624-25630	NN	denotes	liquid
T7185	25645-25653	NN	denotes	counting
T7186	25631-25644	NN	denotes	scintillation
T7187	25654-25659	VBG	denotes	using
T7188	25660-25667	NNP	denotes	Ecolume
T7189	25668-25669	-LRB-	denotes	(
T7190	25673-25687	NNP	denotes	Radiochemicals
T7191	25669-25672	NN	denotes	ICN
T7192	25688-25694	NNP	denotes	Irvine
T7193	25694-25696	,	denotes	,
T7194	25696-25698	NNP	denotes	CA
T7195	25698-25700	,	denotes	,
T7196	25700-25703	NNP	denotes	USA
T7197	25703-25704	-RRB-	denotes	)
T7198	25704-25705	.	denotes	.
T7199	25705-25782	sentence	denotes	Assays were carried out in duplicate with correction for zero-time controls.
T7200	25706-25712	NNS	denotes	Assays
T7201	25718-25725	VBN	denotes	carried
T7202	25713-25717	VBD	denotes	were
T7203	25726-25729	RP	denotes	out
T7204	25730-25732	IN	denotes	in
T7205	25733-25742	NN	denotes	duplicate
T7206	25743-25747	IN	denotes	with
T7207	25748-25758	NN	denotes	correction
T7208	25759-25762	IN	denotes	for
T7209	25763-25767	NN	denotes	zero
T7210	25768-25772	NN	denotes	time
T7211	25767-25768	HYPH	denotes	-
T7212	25773-25781	NNS	denotes	controls
T7213	25781-25782	.	denotes	.
T7214	25782-25919	sentence	denotes	HMG-CoA reductase (EC 1.1.1.3.4) activity was determined according to the method of Xu et al. [35] with modifications as described [36].
T7215	25783-25786	NN	denotes	HMG
T7216	25787-25790	NN	denotes	CoA
T7217	25786-25787	HYPH	denotes	-
T7218	25791-25800	NN	denotes	reductase
T7219	25816-25824	NN	denotes	activity
T7220	25801-25802	-LRB-	denotes	(
T7221	25802-25804	NN	denotes	EC
T7222	25805-25814	CD	denotes	1.1.1.3.4
T7223	25814-25815	-RRB-	denotes	)
T7224	25829-25839	VBN	denotes	determined
T7225	25825-25828	VBD	denotes	was
T7226	25840-25849	VBG	denotes	according
T7227	25850-25852	IN	denotes	to
T7228	25853-25856	DT	denotes	the
T7229	25857-25863	NN	denotes	method
T7230	25864-25866	IN	denotes	of
T7231	25867-25869	NNP	denotes	Xu
T7232	25870-25872	FW	denotes	et
T7233	25873-25876	FW	denotes	al.
T7234	25877-25878	-LRB-	denotes	[
T7235	25878-25880	CD	denotes	35
T7236	25880-25881	-RRB-	denotes	]
T7237	25882-25886	IN	denotes	with
T7238	25887-25900	NNS	denotes	modifications
T7239	25901-25903	IN	denotes	as
T7240	25904-25913	VBN	denotes	described
T7241	25914-25915	-LRB-	denotes	[
T7242	25915-25917	CD	denotes	36
T7243	25917-25918	-RRB-	denotes	]
T7244	25918-25919	.	denotes	.
T7248	25921-25932	JJ	denotes	Statistical
T7249	25933-25941	NN	denotes	analysis
T7250	25941-25971	sentence	denotes	Data are shown as means ± SD.
T7251	25942-25946	NNS	denotes	Data
T7252	25951-25956	VBN	denotes	shown
T7253	25947-25950	VBP	denotes	are
T7254	25957-25959	IN	denotes	as
T7255	25960-25965	NN	denotes	means
T7256	25968-25970	NN	denotes	SD
T7257	25966-25967	SYM	denotes	±
T7258	25970-25971	.	denotes	.
T7259	25971-26089	sentence	denotes	The Student's t test was used to determine the statistical significance of differences between the groups of animals.
T7260	25972-25975	DT	denotes	The
T7261	25976-25983	NNP	denotes	Student
T7262	25988-25992	NN	denotes	test
T7263	25983-25985	POS	denotes	's
T7264	25986-25987	NN	denotes	t
T7265	25997-26001	VBN	denotes	used
T7266	25993-25996	VBD	denotes	was
T7267	26002-26004	TO	denotes	to
T7268	26005-26014	VB	denotes	determine
T7269	26015-26018	DT	denotes	the
T7270	26031-26043	NN	denotes	significance
T7271	26019-26030	JJ	denotes	statistical
T7272	26044-26046	IN	denotes	of
T7273	26047-26058	NNS	denotes	differences
T7274	26059-26066	IN	denotes	between
T7275	26067-26070	DT	denotes	the
T7276	26071-26077	NNS	denotes	groups
T7277	26078-26080	IN	denotes	of
T7278	26081-26088	NNS	denotes	animals
T7279	26088-26089	.	denotes	.
T7280	26089-26129	sentence	denotes	Significance was set at P values <0.05.
T7281	26090-26102	NN	denotes	Significance
T7282	26107-26110	VBN	denotes	set
T7283	26103-26106	VBD	denotes	was
T7284	26111-26113	IN	denotes	at
T7285	26114-26115	NN	denotes	P
T7286	26116-26122	NNS	denotes	values
T7287	26123-26124	SYM	denotes	<
T7288	26124-26128	CD	denotes	0.05
T7289	26128-26129	.	denotes	.
T7460	26140-26149	NN	denotes	Isolation
T7461	26150-26152	IN	denotes	of
T7462	26153-26158	NN	denotes	Abcg8
T7463	26159-26167	NN	denotes	sterolin
T7464	26158-26159	HYPH	denotes	/
T7465	26170-26179	JJ	denotes	deficient
T7466	26167-26168	HYPH	denotes	-
T7467	26168-26169	CD	denotes	2
T7468	26180-26184	NNS	denotes	mice
T7469	26184-26342	sentence	denotes	The targeting construct (Figure 1a) results in the potential disruption of normal splicing, as well as loss of some coding sequences involving exons 3 and 4.
T7470	26185-26188	DT	denotes	The
T7471	26199-26208	NN	denotes	construct
T7472	26189-26198	VBG	denotes	targeting
T7473	26221-26228	VBZ	denotes	results
T7474	26209-26210	-LRB-	denotes	(
T7475	26217-26219	NN	denotes	1a
T7476	26210-26216	NN	denotes	Figure
T7477	26219-26220	-RRB-	denotes	)
T7478	26229-26231	IN	denotes	in
T7479	26232-26235	DT	denotes	the
T7480	26246-26256	NN	denotes	disruption
T7481	26236-26245	JJ	denotes	potential
T7482	26257-26259	IN	denotes	of
T7483	26260-26266	JJ	denotes	normal
T7484	26267-26275	NN	denotes	splicing
T7485	26275-26277	,	denotes	,
T7486	26277-26279	RB	denotes	as
T7487	26285-26287	IN	denotes	as
T7488	26280-26284	RB	denotes	well
T7489	26288-26292	NN	denotes	loss
T7490	26293-26295	IN	denotes	of
T7491	26296-26300	DT	denotes	some
T7492	26308-26317	NNS	denotes	sequences
T7493	26301-26307	VBG	denotes	coding
T7494	26318-26327	VBG	denotes	involving
T7495	26328-26333	NNS	denotes	exons
T7496	26334-26335	CD	denotes	3
T7497	26336-26339	CC	denotes	and
T7498	26340-26341	CD	denotes	4
T7499	26341-26342	.	denotes	.
T7500	26342-26499	sentence	denotes	After ES cell electroporation with the linearized targeted plasmid DNA, two homologous recombinant ES clones (out of 58 ES clones screened) were identified.
T7501	26343-26348	IN	denotes	After
T7502	26488-26498	VBN	denotes	identified
T7503	26349-26351	NN	denotes	ES
T7504	26352-26356	NN	denotes	cell
T7505	26357-26372	NN	denotes	electroporation
T7506	26373-26377	IN	denotes	with
T7507	26378-26381	DT	denotes	the
T7508	26410-26413	NN	denotes	DNA
T7509	26382-26392	VBN	denotes	linearized
T7510	26393-26401	VBN	denotes	targeted
T7511	26402-26409	NN	denotes	plasmid
T7512	26413-26415	,	denotes	,
T7513	26415-26418	CD	denotes	two
T7514	26445-26451	NNS	denotes	clones
T7515	26419-26429	JJ	denotes	homologous
T7516	26430-26441	JJ	denotes	recombinant
T7517	26442-26444	NN	denotes	ES
T7518	26452-26453	-LRB-	denotes	(
T7519	26453-26456	IN	denotes	out
T7520	26457-26459	IN	denotes	of
T7521	26460-26462	CD	denotes	58
T7522	26466-26472	NNS	denotes	clones
T7523	26463-26465	NN	denotes	ES
T7524	26473-26481	VBN	denotes	screened
T7525	26481-26482	-RRB-	denotes	)
T7526	26483-26487	VBD	denotes	were
T7527	26498-26499	.	denotes	.
T7528	26499-26595	sentence	denotes	Blastocyst injection of these resulted in five highly chimeric mice (three males, two females).
T7529	26500-26510	NN	denotes	Blastocyst
T7530	26511-26520	NN	denotes	injection
T7531	26530-26538	VBD	denotes	resulted
T7532	26521-26523	IN	denotes	of
T7533	26524-26529	DT	denotes	these
T7534	26539-26541	IN	denotes	in
T7535	26542-26546	CD	denotes	five
T7536	26563-26567	NNS	denotes	mice
T7537	26547-26553	RB	denotes	highly
T7538	26554-26562	JJ	denotes	chimeric
T7539	26568-26569	-LRB-	denotes	(
T7540	26575-26580	NNS	denotes	males
T7541	26569-26574	CD	denotes	three
T7542	26580-26582	,	denotes	,
T7543	26582-26585	CD	denotes	two
T7544	26586-26593	NNS	denotes	females
T7545	26593-26594	-RRB-	denotes	)
T7546	26594-26595	.	denotes	.
T7547	26595-26781	sentence	denotes	One male and one female, both from the same ES clone, showed germline transmission and the female line was used to establish a multi-generational colony bred onto a C57Bl/6J background.
T7548	26596-26599	CD	denotes	One
T7549	26600-26604	NN	denotes	male
T7550	26650-26656	VBD	denotes	showed
T7551	26605-26608	CC	denotes	and
T7552	26609-26612	CD	denotes	one
T7553	26613-26619	NN	denotes	female
T7554	26619-26621	,	denotes	,
T7555	26621-26625	DT	denotes	both
T7556	26626-26630	IN	denotes	from
T7557	26631-26634	DT	denotes	the
T7558	26643-26648	NN	denotes	clone
T7559	26635-26639	JJ	denotes	same
T7560	26640-26642	NN	denotes	ES
T7561	26648-26650	,	denotes	,
T7562	26657-26665	NN	denotes	germline
T7563	26666-26678	NN	denotes	transmission
T7564	26679-26682	CC	denotes	and
T7565	26683-26686	DT	denotes	the
T7566	26694-26698	NN	denotes	line
T7567	26687-26693	JJ	denotes	female
T7568	26703-26707	VBN	denotes	used
T7569	26699-26702	VBD	denotes	was
T7570	26708-26710	TO	denotes	to
T7571	26711-26720	VB	denotes	establish
T7572	26721-26722	DT	denotes	a
T7573	26742-26748	NN	denotes	colony
T7574	26723-26741	JJ	denotes	multi-generational
T7575	26749-26753	VBN	denotes	bred
T7576	26754-26758	IN	denotes	onto
T7577	26759-26760	DT	denotes	a
T7578	26770-26780	NN	denotes	background
T7579	26761-26766	NN	denotes	C57Bl
T7580	26767-26769	NN	denotes	6J
T7581	26766-26767	HYPH	denotes	/
T7582	26780-26781	.	denotes	.
T7583	26781-26850	sentence	denotes	Heterozygous mice were fertile and gave rise to normal litter sizes.
T7584	26782-26794	JJ	denotes	Heterozygous
T7585	26795-26799	NNS	denotes	mice
T7586	26800-26804	VBD	denotes	were
T7587	26805-26812	JJ	denotes	fertile
T7588	26813-26816	CC	denotes	and
T7589	26817-26821	VBD	denotes	gave
T7590	26822-26826	NN	denotes	rise
T7591	26827-26829	IN	denotes	to
T7592	26830-26836	JJ	denotes	normal
T7593	26844-26849	NNS	denotes	sizes
T7594	26837-26843	JJ	denotes	litter
T7595	26849-26850	.	denotes	.
T7596	26850-26997	sentence	denotes	Breeding heterozygous animals led to wild-type, heterozygous and homozygous knockout mice in the expected Mendelian distribution (data not shown).
T7597	26851-26859	VBG	denotes	Breeding
T7598	26881-26884	VBD	denotes	led
T7599	26860-26872	JJ	denotes	heterozygous
T7600	26873-26880	NNS	denotes	animals
T7601	26885-26887	IN	denotes	to
T7602	26888-26892	JJ	denotes	wild
T7603	26893-26897	NN	denotes	type
T7604	26892-26893	HYPH	denotes	-
T7605	26936-26940	NNS	denotes	mice
T7606	26897-26899	,	denotes	,
T7607	26899-26911	JJ	denotes	heterozygous
T7608	26927-26935	NN	denotes	knockout
T7609	26912-26915	CC	denotes	and
T7610	26916-26926	JJ	denotes	homozygous
T7611	26941-26943	IN	denotes	in
T7612	26944-26947	DT	denotes	the
T7613	26967-26979	NN	denotes	distribution
T7614	26948-26956	VBN	denotes	expected
T7615	26957-26966	JJ	denotes	Mendelian
T7616	26980-26981	-LRB-	denotes	(
T7617	26990-26995	VBN	denotes	shown
T7618	26981-26985	NNS	denotes	data
T7619	26986-26989	RB	denotes	not
T7620	26995-26996	-RRB-	denotes	)
T7621	26996-26997	.	denotes	.
T7622	26997-27167	sentence	denotes	Although not quantitative, Northern analyses showed that while the knockout mice showed no detectable mRNA for Abcg8, expression of Abcg5 appeared unaltered (Figure 1c).
T7623	26998-27006	IN	denotes	Although
T7624	27011-27023	JJ	denotes	quantitative
T7625	27007-27010	RB	denotes	not
T7626	27043-27049	VBD	denotes	showed
T7627	27023-27025	,	denotes	,
T7628	27025-27033	NNP	denotes	Northern
T7629	27034-27042	NNS	denotes	analyses
T7630	27050-27054	IN	denotes	that
T7631	27136-27144	VBD	denotes	appeared
T7632	27055-27060	IN	denotes	while
T7633	27079-27085	VBD	denotes	showed
T7634	27061-27064	DT	denotes	the
T7635	27074-27078	NNS	denotes	mice
T7636	27065-27073	NN	denotes	knockout
T7637	27086-27088	DT	denotes	no
T7638	27100-27104	NN	denotes	mRNA
T7639	27089-27099	JJ	denotes	detectable
T7640	27105-27108	IN	denotes	for
T7641	27109-27114	NN	denotes	Abcg8
T7642	27114-27116	,	denotes	,
T7643	27116-27126	NN	denotes	expression
T7644	27127-27129	IN	denotes	of
T7645	27130-27135	NN	denotes	Abcg5
T7646	27145-27154	JJ	denotes	unaltered
T7647	27155-27156	-LRB-	denotes	(
T7648	27163-27165	NN	denotes	1c
T7649	27156-27162	NN	denotes	Figure
T7650	27165-27166	-RRB-	denotes	)
T7651	27166-27167	.	denotes	.
T7652	27167-27446	sentence	denotes	To exclude the possibility of alternative splicing with the production of a non-functional but truncated Abcg8/sterolin-2 protein that may serve as a chaperone for Abcg5/sterolin-1, RT-PCR was performed on cDNA reverse transcribed from total liver RNA from Abcg8-deficient mice.
T7653	27168-27170	TO	denotes	To
T7654	27171-27178	VB	denotes	exclude
T7655	27361-27370	VBN	denotes	performed
T7656	27179-27182	DT	denotes	the
T7657	27183-27194	NN	denotes	possibility
T7658	27195-27197	IN	denotes	of
T7659	27198-27209	JJ	denotes	alternative
T7660	27210-27218	NN	denotes	splicing
T7661	27219-27223	IN	denotes	with
T7662	27224-27227	DT	denotes	the
T7663	27228-27238	NN	denotes	production
T7664	27239-27241	IN	denotes	of
T7665	27242-27243	DT	denotes	a
T7666	27290-27297	NN	denotes	protein
T7667	27244-27258	JJ	denotes	non-functional
T7668	27259-27262	CC	denotes	but
T7669	27263-27272	VBN	denotes	truncated
T7670	27273-27278	NN	denotes	Abcg8
T7671	27279-27287	NN	denotes	sterolin
T7672	27278-27279	HYPH	denotes	/
T7673	27287-27288	HYPH	denotes	-
T7674	27288-27289	CD	denotes	2
T7675	27298-27302	WDT	denotes	that
T7676	27307-27312	VB	denotes	serve
T7677	27303-27306	MD	denotes	may
T7678	27313-27315	IN	denotes	as
T7679	27316-27317	DT	denotes	a
T7680	27318-27327	NN	denotes	chaperone
T7681	27328-27331	IN	denotes	for
T7682	27332-27337	NN	denotes	Abcg5
T7683	27338-27346	NN	denotes	sterolin
T7684	27337-27338	HYPH	denotes	/
T7685	27346-27347	HYPH	denotes	-
T7686	27347-27348	CD	denotes	1
T7687	27348-27350	,	denotes	,
T7688	27350-27352	NN	denotes	RT
T7689	27353-27356	NN	denotes	PCR
T7690	27352-27353	HYPH	denotes	-
T7691	27357-27360	VBD	denotes	was
T7692	27371-27373	IN	denotes	on
T7693	27374-27378	NN	denotes	cDNA
T7694	27379-27386	RB	denotes	reverse
T7695	27387-27398	VBN	denotes	transcribed
T7696	27399-27403	IN	denotes	from
T7697	27404-27409	JJ	denotes	total
T7698	27416-27419	NN	denotes	RNA
T7699	27410-27415	NN	denotes	liver
T7700	27420-27424	IN	denotes	from
T7701	27425-27430	NN	denotes	Abcg8
T7702	27431-27440	JJ	denotes	deficient
T7703	27430-27431	HYPH	denotes	-
T7704	27441-27445	NNS	denotes	mice
T7705	27445-27446	.	denotes	.
T7706	27446-27661	sentence	denotes	No mRNA for Abcg8/sterolin-2 was detected containing any sequences downstream of exon 4 in the Abcg8-deficient mice, whether primers were located in exons 4 and 13, exons 9 and 13 or exons 10 and 13 (Figure 1d,1e).
T7707	27447-27449	DT	denotes	No
T7708	27450-27454	NN	denotes	mRNA
T7709	27489-27499	VBG	denotes	containing
T7710	27455-27458	IN	denotes	for
T7711	27459-27464	NN	denotes	Abcg8
T7712	27465-27473	NN	denotes	sterolin
T7713	27464-27465	HYPH	denotes	/
T7714	27473-27474	HYPH	denotes	-
T7715	27474-27475	CD	denotes	2
T7716	27476-27479	VBD	denotes	was
T7717	27480-27488	VBN	denotes	detected
T7718	27500-27503	DT	denotes	any
T7719	27504-27513	NNS	denotes	sequences
T7720	27514-27524	RB	denotes	downstream
T7721	27525-27527	IN	denotes	of
T7722	27528-27532	NN	denotes	exon
T7723	27533-27534	CD	denotes	4
T7724	27535-27537	IN	denotes	in
T7725	27538-27541	DT	denotes	the
T7726	27558-27562	NNS	denotes	mice
T7727	27542-27547	NN	denotes	Abcg8
T7728	27548-27557	JJ	denotes	deficient
T7729	27547-27548	HYPH	denotes	-
T7730	27562-27564	,	denotes	,
T7731	27564-27571	IN	denotes	whether
T7732	27585-27592	VBN	denotes	located
T7733	27572-27579	NNS	denotes	primers
T7734	27580-27584	VBD	denotes	were
T7735	27593-27595	IN	denotes	in
T7736	27596-27601	NNS	denotes	exons
T7737	27602-27603	CD	denotes	4
T7738	27604-27607	CC	denotes	and
T7739	27608-27610	CD	denotes	13
T7740	27610-27612	,	denotes	,
T7741	27612-27617	NNS	denotes	exons
T7742	27618-27619	CD	denotes	9
T7743	27620-27623	CC	denotes	and
T7744	27624-27626	CD	denotes	13
T7745	27627-27629	CC	denotes	or
T7746	27630-27635	NNS	denotes	exons
T7747	27636-27638	CD	denotes	10
T7748	27639-27642	CC	denotes	and
T7749	27643-27645	CD	denotes	13
T7750	27646-27647	-LRB-	denotes	(
T7751	27657-27659	NN	denotes	1e
T7752	27647-27653	NN	denotes	Figure
T7753	27654-27656	NN	denotes	1d
T7754	27656-27657	,	denotes	,
T7755	27659-27660	-RRB-	denotes	)
T7756	27660-27661	.	denotes	.
T7757	27661-27818	sentence	denotes	This demonstrates that if an alternatively spliced message could potentially code for a truncated protein, it is not detectable in the Abcg8-deficient mice.
T7758	27662-27666	DT	denotes	This
T7759	27667-27679	VBZ	denotes	demonstrates
T7760	27680-27684	IN	denotes	that
T7761	27772-27774	VBZ	denotes	is
T7762	27685-27687	IN	denotes	if
T7763	27739-27743	VB	denotes	code
T7764	27688-27690	DT	denotes	an
T7765	27713-27720	NN	denotes	message
T7766	27691-27704	RB	denotes	alternatively
T7767	27705-27712	VBN	denotes	spliced
T7768	27721-27726	MD	denotes	could
T7769	27727-27738	RB	denotes	potentially
T7770	27744-27747	IN	denotes	for
T7771	27748-27749	DT	denotes	a
T7772	27760-27767	NN	denotes	protein
T7773	27750-27759	VBN	denotes	truncated
T7774	27767-27769	,	denotes	,
T7775	27769-27771	PRP	denotes	it
T7776	27775-27778	RB	denotes	not
T7777	27779-27789	JJ	denotes	detectable
T7778	27790-27792	IN	denotes	in
T7779	27793-27796	DT	denotes	the
T7780	27813-27817	NNS	denotes	mice
T7781	27797-27802	NN	denotes	Abcg8
T7782	27803-27812	JJ	denotes	deficient
T7783	27802-27803	HYPH	denotes	-
T7784	27817-27818	.	denotes	.
T7785	27818-27895	sentence	denotes	The membrane-spanning domains of Abcg8/sterolin-2 are encoded by exons 9–13.
T7786	27819-27822	DT	denotes	The
T7787	27841-27848	NNS	denotes	domains
T7788	27823-27831	NN	denotes	membrane
T7789	27832-27840	VBG	denotes	spanning
T7790	27831-27832	HYPH	denotes	-
T7791	27873-27880	VBN	denotes	encoded
T7792	27849-27851	IN	denotes	of
T7793	27852-27857	NN	denotes	Abcg8
T7794	27858-27866	NN	denotes	sterolin
T7795	27857-27858	HYPH	denotes	/
T7796	27866-27867	HYPH	denotes	-
T7797	27867-27868	CD	denotes	2
T7798	27869-27872	VBP	denotes	are
T7799	27881-27883	IN	denotes	by
T7800	27884-27889	NNS	denotes	exons
T7801	27890-27891	CD	denotes	9
T7802	27891-27892	SYM	denotes	–
T7803	27892-27894	CD	denotes	13
T7804	27894-27895	.	denotes	.
T8132	27897-27903	NN	denotes	Sterol
T8133	27904-27910	NNS	denotes	levels
T8134	27911-27913	IN	denotes	in
T8135	27914-27919	NN	denotes	Abcg8
T8136	27920-27928	NN	denotes	sterolin
T8137	27919-27920	HYPH	denotes	/
T8138	27931-27940	JJ	denotes	deficient
T8139	27928-27929	HYPH	denotes	-
T8140	27929-27930	CD	denotes	2
T8141	27941-27948	NNS	denotes	animals
T8142	27948-28026	sentence	denotes	Elevated plant sterols in tissue and plasma are diagnostic of sitosterolemia.
T8143	27949-27957	JJ	denotes	Elevated
T8144	27964-27971	NNS	denotes	sterols
T8145	27958-27963	NN	denotes	plant
T8146	27993-27996	VBP	denotes	are
T8147	27972-27974	IN	denotes	in
T8148	27975-27981	NN	denotes	tissue
T8149	27982-27985	CC	denotes	and
T8150	27986-27992	NN	denotes	plasma
T8151	27997-28007	JJ	denotes	diagnostic
T8152	28008-28010	IN	denotes	of
T8153	28011-28025	NN	denotes	sitosterolemia
T8154	28025-28026	.	denotes	.
T8155	28026-28157	sentence	denotes	Sterol levels, as determined by gas chromatography (GC) analysis, in plasma and tissues of the Abcg8-/- mice are shown in Table 2.
T8156	28027-28033	NN	denotes	Sterol
T8157	28034-28040	NNS	denotes	levels
T8158	28140-28145	VBN	denotes	shown
T8159	28040-28042	,	denotes	,
T8160	28042-28044	IN	denotes	as
T8161	28045-28055	VBN	denotes	determined
T8162	28056-28058	IN	denotes	by
T8163	28059-28062	NN	denotes	gas
T8164	28063-28077	NN	denotes	chromatography
T8165	28083-28091	NN	denotes	analysis
T8166	28078-28079	-LRB-	denotes	(
T8167	28079-28081	NN	denotes	GC
T8168	28081-28082	-RRB-	denotes	)
T8169	28091-28093	,	denotes	,
T8170	28093-28095	IN	denotes	in
T8171	28096-28102	NN	denotes	plasma
T8172	28103-28106	CC	denotes	and
T8173	28107-28114	NNS	denotes	tissues
T8174	28115-28117	IN	denotes	of
T8175	28118-28121	DT	denotes	the
T8176	28131-28135	NNS	denotes	mice
T8177	28122-28127	NN	denotes	Abcg8
T8178	28127-28128	SYM	denotes	-
T8179	28128-28129	HYPH	denotes	/
T8180	28129-28130	SYM	denotes	-
T8181	28136-28139	VBP	denotes	are
T8182	28146-28148	IN	denotes	in
T8183	28149-28154	NN	denotes	Table
T8184	28155-28156	CD	denotes	2
T8185	28156-28157	.	denotes	.
T8186	28157-28250	sentence	denotes	There were no differences in tissue weights between wild types, heterozygotes and knockouts.
T8187	28158-28163	EX	denotes	There
T8188	28164-28168	VBD	denotes	were
T8189	28169-28171	DT	denotes	no
T8190	28172-28183	NNS	denotes	differences
T8191	28184-28186	IN	denotes	in
T8192	28187-28193	NN	denotes	tissue
T8193	28194-28201	NNS	denotes	weights
T8194	28202-28209	IN	denotes	between
T8195	28210-28214	JJ	denotes	wild
T8196	28215-28220	NNS	denotes	types
T8197	28220-28222	,	denotes	,
T8198	28222-28235	NNS	denotes	heterozygotes
T8199	28236-28239	CC	denotes	and
T8200	28240-28249	NNS	denotes	knockouts
T8201	28249-28250	.	denotes	.
T8202	28250-28426	sentence	denotes	Plasma cholesterol levels of the homozygous and heterozygous mice were decreased by 52% and 26%, respectively, compared to those of wild-type mice (n = 3 all groups, Table 2).
T8203	28251-28257	NN	denotes	Plasma
T8204	28258-28269	NN	denotes	cholesterol
T8205	28270-28276	NNS	denotes	levels
T8206	28322-28331	VBN	denotes	decreased
T8207	28277-28279	IN	denotes	of
T8208	28280-28283	DT	denotes	the
T8209	28312-28316	NNS	denotes	mice
T8210	28284-28294	JJ	denotes	homozygous
T8211	28295-28298	CC	denotes	and
T8212	28299-28311	JJ	denotes	heterozygous
T8213	28317-28321	VBD	denotes	were
T8214	28332-28334	IN	denotes	by
T8215	28335-28337	CD	denotes	52
T8216	28337-28338	NN	denotes	%
T8217	28339-28342	CC	denotes	and
T8218	28343-28345	CD	denotes	26
T8219	28345-28346	NN	denotes	%
T8220	28346-28348	,	denotes	,
T8221	28348-28360	RB	denotes	respectively
T8222	28360-28362	,	denotes	,
T8223	28362-28370	VBN	denotes	compared
T8224	28371-28373	IN	denotes	to
T8225	28374-28379	DT	denotes	those
T8226	28380-28382	IN	denotes	of
T8227	28383-28387	JJ	denotes	wild
T8228	28388-28392	NN	denotes	type
T8229	28387-28388	HYPH	denotes	-
T8230	28393-28397	NNS	denotes	mice
T8231	28398-28399	-LRB-	denotes	(
T8232	28417-28422	NN	denotes	Table
T8233	28399-28400	NN	denotes	n
T8234	28403-28404	CD	denotes	3
T8235	28401-28402	SYM	denotes	=
T8236	28405-28408	DT	denotes	all
T8237	28409-28415	NNS	denotes	groups
T8238	28415-28417	,	denotes	,
T8239	28423-28424	CD	denotes	2
T8240	28424-28425	-RRB-	denotes	)
T8241	28425-28426	.	denotes	.
T8242	28426-28551	sentence	denotes	Plant sterols were almost undetectable in wild-type and heterozygous mice, but were significantly elevated in Abcg8-/- mice.
T8243	28427-28432	NN	denotes	Plant
T8244	28433-28440	NNS	denotes	sterols
T8245	28441-28445	VBD	denotes	were
T8246	28446-28452	RB	denotes	almost
T8247	28453-28465	JJ	denotes	undetectable
T8248	28466-28468	IN	denotes	in
T8249	28469-28473	JJ	denotes	wild
T8250	28474-28478	NN	denotes	type
T8251	28473-28474	HYPH	denotes	-
T8252	28496-28500	NNS	denotes	mice
T8253	28479-28482	CC	denotes	and
T8254	28483-28495	JJ	denotes	heterozygous
T8255	28500-28502	,	denotes	,
T8256	28502-28505	CC	denotes	but
T8257	28506-28510	VBD	denotes	were
T8258	28525-28533	VBN	denotes	elevated
T8259	28511-28524	RB	denotes	significantly
T8260	28534-28536	IN	denotes	in
T8261	28537-28542	NN	denotes	Abcg8
T8262	28546-28550	NNS	denotes	mice
T8263	28542-28543	SYM	denotes	-
T8264	28543-28544	HYPH	denotes	/
T8265	28544-28545	SYM	denotes	-
T8266	28550-28551	.	denotes	.
T8267	28551-28761	sentence	denotes	Plasma campesterol and sitosterol levels were eight- and 36-fold higher in Abcg8-/- mice compared to wild-type mice (8.76 ± 2.10 and 18.52 ± 5.03 mg/dL, respectively, versus 1.14 ± 0.69 and 0.50 ± 0.55 mg/dL).
T8268	28552-28558	NN	denotes	Plasma
T8269	28586-28592	NNS	denotes	levels
T8270	28559-28570	NN	denotes	campesterol
T8271	28571-28574	CC	denotes	and
T8272	28575-28585	NN	denotes	sitosterol
T8273	28593-28597	VBD	denotes	were
T8274	28598-28603	CD	denotes	eight
T8275	28617-28623	JJR	denotes	higher
T8276	28603-28604	HYPH	denotes	-
T8277	28605-28608	CC	denotes	and
T8278	28609-28616	RB	denotes	36-fold
T8279	28624-28626	IN	denotes	in
T8280	28627-28632	NN	denotes	Abcg8
T8281	28636-28640	NNS	denotes	mice
T8282	28632-28633	SYM	denotes	-
T8283	28633-28634	HYPH	denotes	/
T8284	28634-28635	SYM	denotes	-
T8285	28641-28649	VBN	denotes	compared
T8286	28650-28652	IN	denotes	to
T8287	28653-28657	JJ	denotes	wild
T8288	28658-28662	NN	denotes	type
T8289	28657-28658	HYPH	denotes	-
T8290	28663-28667	NNS	denotes	mice
T8291	28668-28669	-LRB-	denotes	(
T8292	28698-28700	NN	denotes	mg
T8293	28669-28673	CD	denotes	8.76
T8294	28676-28680	CD	denotes	2.10
T8295	28674-28675	SYM	denotes	±
T8296	28681-28684	CC	denotes	and
T8297	28685-28690	CD	denotes	18.52
T8298	28693-28697	CD	denotes	5.03
T8299	28691-28692	SYM	denotes	±
T8300	28700-28701	SYM	denotes	/
T8301	28701-28703	NN	denotes	dL
T8302	28703-28705	,	denotes	,
T8303	28705-28717	RB	denotes	respectively
T8304	28717-28719	,	denotes	,
T8305	28719-28725	CC	denotes	versus
T8306	28726-28730	CD	denotes	1.14
T8307	28733-28737	CD	denotes	0.69
T8308	28731-28732	SYM	denotes	±
T8309	28754-28756	NN	denotes	mg
T8310	28738-28741	CC	denotes	and
T8311	28742-28746	CD	denotes	0.50
T8312	28749-28753	CD	denotes	0.55
T8313	28747-28748	SYM	denotes	±
T8314	28756-28757	SYM	denotes	/
T8315	28757-28759	NN	denotes	dL
T8316	28759-28760	-RRB-	denotes	)
T8317	28760-28761	.	denotes	.
T8318	28761-28872	sentence	denotes	There was no significant difference in the plasma plant sterol levels between heterozygous and wild-type mice.
T8319	28762-28767	EX	denotes	There
T8320	28768-28771	VBD	denotes	was
T8321	28772-28774	DT	denotes	no
T8322	28787-28797	NN	denotes	difference
T8323	28775-28786	JJ	denotes	significant
T8324	28798-28800	IN	denotes	in
T8325	28801-28804	DT	denotes	the
T8326	28825-28831	NNS	denotes	levels
T8327	28805-28811	NN	denotes	plasma
T8328	28818-28824	NN	denotes	sterol
T8329	28812-28817	NN	denotes	plant
T8330	28832-28839	IN	denotes	between
T8331	28840-28852	JJ	denotes	heterozygous
T8332	28867-28871	NNS	denotes	mice
T8333	28853-28856	CC	denotes	and
T8334	28857-28861	JJ	denotes	wild
T8335	28862-28866	NN	denotes	type
T8336	28861-28862	HYPH	denotes	-
T8337	28871-28872	.	denotes	.
T8338	28872-29060	sentence	denotes	Liver cholesterol content of the Abcg8-/- mice was reduced by ~50% relative to wild-type liver (1202 ± 264 μg/g wet weight tissue, versus 2280 ± 310 μg/g wet weight tissue, respectively).
T8339	28873-28878	NN	denotes	Liver
T8340	28879-28890	NN	denotes	cholesterol
T8341	28891-28898	NN	denotes	content
T8342	28924-28931	VBN	denotes	reduced
T8343	28899-28901	IN	denotes	of
T8344	28902-28905	DT	denotes	the
T8345	28915-28919	NNS	denotes	mice
T8346	28906-28911	NN	denotes	Abcg8
T8347	28911-28912	SYM	denotes	-
T8348	28912-28913	HYPH	denotes	/
T8349	28913-28914	SYM	denotes	-
T8350	28920-28923	VBD	denotes	was
T8351	28932-28934	IN	denotes	by
T8352	28935-28936	SYM	denotes	~
T8353	28936-28938	CD	denotes	50
T8354	28938-28939	NN	denotes	%
T8355	28940-28948	JJ	denotes	relative
T8356	28949-28951	IN	denotes	to
T8357	28952-28956	JJ	denotes	wild
T8358	28957-28961	NN	denotes	type
T8359	28956-28957	HYPH	denotes	-
T8360	28962-28967	NN	denotes	liver
T8361	28968-28969	-LRB-	denotes	(
T8362	28980-28982	NN	denotes	μg
T8363	28969-28973	CD	denotes	1202
T8364	28976-28979	CD	denotes	264
T8365	28974-28975	SYM	denotes	±
T8366	28982-28983	SYM	denotes	/
T8367	28996-29002	NN	denotes	tissue
T8368	28983-28984	NN	denotes	g
T8369	28985-28988	JJ	denotes	wet
T8370	28989-28995	NN	denotes	weight
T8371	29002-29004	,	denotes	,
T8372	29004-29010	CC	denotes	versus
T8373	29011-29015	CD	denotes	2280
T8374	29018-29021	CD	denotes	310
T8375	29016-29017	SYM	denotes	±
T8376	29022-29024	NN	denotes	μg
T8377	29024-29025	SYM	denotes	/
T8378	29038-29044	NN	denotes	tissue
T8379	29025-29026	NN	denotes	g
T8380	29027-29030	JJ	denotes	wet
T8381	29031-29037	NN	denotes	weight
T8382	29044-29046	,	denotes	,
T8383	29046-29058	RB	denotes	respectively
T8384	29058-29059	-RRB-	denotes	)
T8385	29059-29060	.	denotes	.
T8386	29060-29377	sentence	denotes	The reduced cholesterol content was offset by a five-fold increase in campesterol and a 22-fold increase in sitosterol (233.9 ± 48.1 and 376.3 ± 96.1 μg/g wet weight tissue, respectively) in Abcg8-/- knockout mice compared to wild-type mice (52.2 ± 15.0 and 16.7 ± 8.6 μg/g wet weight tissue, respectively, Table 2).
T8387	29061-29064	DT	denotes	The
T8388	29085-29092	NN	denotes	content
T8389	29065-29072	VBN	denotes	reduced
T8390	29073-29084	NN	denotes	cholesterol
T8391	29097-29103	VBN	denotes	offset
T8392	29093-29096	VBD	denotes	was
T8393	29104-29106	IN	denotes	by
T8394	29107-29108	DT	denotes	a
T8395	29119-29127	NN	denotes	increase
T8396	29109-29118	RB	denotes	five-fold
T8397	29128-29130	IN	denotes	in
T8398	29131-29142	NN	denotes	campesterol
T8399	29143-29146	CC	denotes	and
T8400	29147-29148	DT	denotes	a
T8401	29157-29165	NN	denotes	increase
T8402	29149-29156	RB	denotes	22-fold
T8403	29166-29168	IN	denotes	in
T8404	29169-29179	NN	denotes	sitosterol
T8405	29180-29181	-LRB-	denotes	(
T8406	29211-29213	NN	denotes	μg
T8407	29181-29186	CD	denotes	233.9
T8408	29189-29193	CD	denotes	48.1
T8409	29187-29188	SYM	denotes	±
T8410	29194-29197	CC	denotes	and
T8411	29198-29203	CD	denotes	376.3
T8412	29206-29210	CD	denotes	96.1
T8413	29204-29205	SYM	denotes	±
T8414	29213-29214	SYM	denotes	/
T8415	29227-29233	NN	denotes	tissue
T8416	29214-29215	NN	denotes	g
T8417	29216-29219	JJ	denotes	wet
T8418	29220-29226	NN	denotes	weight
T8419	29233-29235	,	denotes	,
T8420	29235-29247	RB	denotes	respectively
T8421	29247-29248	-RRB-	denotes	)
T8422	29249-29251	IN	denotes	in
T8423	29252-29257	NN	denotes	Abcg8
T8424	29261-29269	NN	denotes	knockout
T8425	29257-29258	SYM	denotes	-
T8426	29258-29259	HYPH	denotes	/
T8427	29259-29260	SYM	denotes	-
T8428	29270-29274	NNS	denotes	mice
T8429	29275-29283	VBN	denotes	compared
T8430	29284-29286	IN	denotes	to
T8431	29287-29291	JJ	denotes	wild
T8432	29292-29296	NN	denotes	type
T8433	29291-29292	HYPH	denotes	-
T8434	29297-29301	NNS	denotes	mice
T8435	29302-29303	-LRB-	denotes	(
T8436	29368-29373	NN	denotes	Table
T8437	29303-29307	CD	denotes	52.2
T8438	29310-29314	CD	denotes	15.0
T8439	29308-29309	SYM	denotes	±
T8440	29330-29332	NN	denotes	μg
T8441	29315-29318	CC	denotes	and
T8442	29319-29323	CD	denotes	16.7
T8443	29326-29329	CD	denotes	8.6
T8444	29324-29325	SYM	denotes	±
T8445	29332-29333	SYM	denotes	/
T8446	29346-29352	NN	denotes	tissue
T8447	29333-29334	NN	denotes	g
T8448	29335-29338	JJ	denotes	wet
T8449	29339-29345	NN	denotes	weight
T8450	29352-29354	,	denotes	,
T8451	29354-29366	RB	denotes	respectively
T8452	29366-29368	,	denotes	,
T8453	29374-29375	CD	denotes	2
T8454	29375-29376	-RRB-	denotes	)
T8455	29376-29377	.	denotes	.
T8456	29377-29497	sentence	denotes	There were no significant differences of liver cholesterol or plant sterol contents between Abcg8+/+ and Abcg8+/- mice.
T8457	29378-29383	EX	denotes	There
T8458	29384-29388	VBD	denotes	were
T8459	29389-29391	DT	denotes	no
T8460	29404-29415	NNS	denotes	differences
T8461	29392-29403	JJ	denotes	significant
T8462	29416-29418	IN	denotes	of
T8463	29419-29424	NN	denotes	liver
T8464	29425-29436	NN	denotes	cholesterol
T8465	29453-29461	NNS	denotes	contents
T8466	29437-29439	CC	denotes	or
T8467	29440-29445	NN	denotes	plant
T8468	29446-29452	NN	denotes	sterol
T8469	29462-29469	IN	denotes	between
T8470	29470-29475	NN	denotes	Abcg8
T8471	29492-29496	NNS	denotes	mice
T8472	29475-29476	SYM	denotes	+
T8473	29476-29477	HYPH	denotes	/
T8474	29477-29478	SYM	denotes	+
T8475	29479-29482	CC	denotes	and
T8476	29483-29488	NN	denotes	Abcg8
T8477	29488-29489	SYM	denotes	+
T8478	29489-29490	HYPH	denotes	/
T8479	29490-29491	SYM	denotes	-
T8480	29496-29497	.	denotes	.
T8481	29497-29560	sentence	denotes	Spleen sterol contents reflected the liver profiles (Table 2).
T8482	29498-29504	NN	denotes	Spleen
T8483	29505-29511	NN	denotes	sterol
T8484	29512-29520	NNS	denotes	contents
T8485	29521-29530	VBD	denotes	reflected
T8486	29531-29534	DT	denotes	the
T8487	29541-29549	NNS	denotes	profiles
T8488	29535-29540	NN	denotes	liver
T8489	29550-29551	-LRB-	denotes	(
T8490	29551-29556	NN	denotes	Table
T8491	29557-29558	CD	denotes	2
T8492	29558-29559	-RRB-	denotes	)
T8493	29559-29560	.	denotes	.
T8494	29560-29754	sentence	denotes	No significant differences in brain sterol contents were observed, as would be expected, since the blood brain barrier is intact and prevents entry of these sterols in sitosterolemia (Table 2).
T8495	29561-29563	DT	denotes	No
T8496	29576-29587	NNS	denotes	differences
T8497	29564-29575	JJ	denotes	significant
T8498	29618-29626	VBN	denotes	observed
T8499	29588-29590	IN	denotes	in
T8500	29591-29596	NN	denotes	brain
T8501	29604-29612	NNS	denotes	contents
T8502	29597-29603	NN	denotes	sterol
T8503	29613-29617	VBD	denotes	were
T8504	29626-29628	,	denotes	,
T8505	29628-29630	IN	denotes	as
T8506	29640-29648	VBN	denotes	expected
T8507	29631-29636	MD	denotes	would
T8508	29637-29639	VB	denotes	be
T8509	29648-29650	,	denotes	,
T8510	29650-29655	IN	denotes	since
T8511	29680-29682	VBZ	denotes	is
T8512	29656-29659	DT	denotes	the
T8513	29672-29679	NN	denotes	barrier
T8514	29660-29665	NN	denotes	blood
T8515	29666-29671	NN	denotes	brain
T8516	29683-29689	JJ	denotes	intact
T8517	29690-29693	CC	denotes	and
T8518	29694-29702	VBZ	denotes	prevents
T8519	29703-29708	NN	denotes	entry
T8520	29709-29711	IN	denotes	of
T8521	29712-29717	DT	denotes	these
T8522	29718-29725	NNS	denotes	sterols
T8523	29726-29728	IN	denotes	in
T8524	29729-29743	NN	denotes	sitosterolemia
T8525	29744-29745	-LRB-	denotes	(
T8526	29745-29750	NN	denotes	Table
T8527	29751-29752	CD	denotes	2
T8528	29752-29753	-RRB-	denotes	)
T8529	29753-29754	.	denotes	.
T8530	29754-29888	sentence	denotes	There were only traces of plant sterols in the brains from knockout animals, reflecting blood contamination during tissue harvesting.
T8531	29755-29760	EX	denotes	There
T8532	29761-29765	VBD	denotes	were
T8533	29766-29770	RB	denotes	only
T8534	29771-29777	NNS	denotes	traces
T8535	29778-29780	IN	denotes	of
T8536	29781-29786	NN	denotes	plant
T8537	29787-29794	NNS	denotes	sterols
T8538	29795-29797	IN	denotes	in
T8539	29798-29801	DT	denotes	the
T8540	29802-29808	NNS	denotes	brains
T8541	29809-29813	IN	denotes	from
T8542	29814-29822	NN	denotes	knockout
T8543	29823-29830	NNS	denotes	animals
T8544	29830-29832	,	denotes	,
T8545	29832-29842	VBG	denotes	reflecting
T8546	29843-29848	NN	denotes	blood
T8547	29849-29862	NN	denotes	contamination
T8548	29863-29869	IN	denotes	during
T8549	29870-29876	NN	denotes	tissue
T8550	29877-29887	NN	denotes	harvesting
T8551	29887-29888	.	denotes	.
T8552	29888-29971	sentence	denotes	Interestingly, the majority of the elevated tissue plant sterols are unesterified.
T8553	29889-29902	RB	denotes	Interestingly
T8554	29954-29957	VBP	denotes	are
T8555	29902-29904	,	denotes	,
T8556	29904-29907	DT	denotes	the
T8557	29908-29916	NN	denotes	majority
T8558	29917-29919	IN	denotes	of
T8559	29920-29923	DT	denotes	the
T8560	29946-29953	NNS	denotes	sterols
T8561	29924-29932	VBN	denotes	elevated
T8562	29933-29939	NN	denotes	tissue
T8563	29940-29945	NN	denotes	plant
T8564	29958-29970	JJ	denotes	unesterified
T8565	29970-29971	.	denotes	.
T8566	29971-30082	sentence	denotes	The livers of Abcg8+/+, +/- and -/- mice have relatively similar levels of esterified cholesterol (Figure 2a).
T8567	29972-29975	DT	denotes	The
T8568	29976-29982	NNS	denotes	livers
T8569	30013-30017	VBP	denotes	have
T8570	29983-29985	IN	denotes	of
T8571	29986-29991	NN	denotes	Abcg8
T8572	29993-29994	SYM	denotes	+
T8573	29991-29992	SYM	denotes	+
T8574	29992-29993	HYPH	denotes	/
T8575	30008-30012	NNS	denotes	mice
T8576	29994-29996	,	denotes	,
T8577	29996-29997	SYM	denotes	+
T8578	29998-29999	SYM	denotes	-
T8579	29997-29998	HYPH	denotes	/
T8580	30000-30003	CC	denotes	and
T8581	30004-30005	SYM	denotes	-
T8582	30006-30007	SYM	denotes	-
T8583	30005-30006	HYPH	denotes	/
T8584	30018-30028	RB	denotes	relatively
T8585	30029-30036	JJ	denotes	similar
T8586	30037-30043	NNS	denotes	levels
T8587	30044-30046	IN	denotes	of
T8588	30047-30057	JJ	denotes	esterified
T8589	30058-30069	NN	denotes	cholesterol
T8590	30070-30071	-LRB-	denotes	(
T8591	30078-30080	NN	denotes	2a
T8592	30071-30077	NN	denotes	Figure
T8593	30080-30081	-RRB-	denotes	)
T8594	30081-30082	.	denotes	.
T8595	30082-30216	sentence	denotes	However, there is little if any esterification of sitosterol or campesterol (Figure 2b,2c) consistent with previous findings [37,38].
T8596	30083-30090	RB	denotes	However
T8597	30098-30100	VBZ	denotes	is
T8598	30090-30092	,	denotes	,
T8599	30092-30097	EX	denotes	there
T8600	30101-30107	JJ	denotes	little
T8601	30115-30129	NN	denotes	esterification
T8602	30108-30110	IN	denotes	if
T8603	30111-30114	DT	denotes	any
T8604	30130-30132	IN	denotes	of
T8605	30133-30143	NN	denotes	sitosterol
T8606	30144-30146	CC	denotes	or
T8607	30147-30158	NN	denotes	campesterol
T8608	30159-30160	-LRB-	denotes	(
T8609	30170-30172	NN	denotes	2c
T8610	30160-30166	NN	denotes	Figure
T8611	30167-30169	NN	denotes	2b
T8612	30169-30170	,	denotes	,
T8613	30172-30173	-RRB-	denotes	)
T8614	30174-30184	JJ	denotes	consistent
T8615	30185-30189	IN	denotes	with
T8616	30190-30198	JJ	denotes	previous
T8617	30199-30207	NNS	denotes	findings
T8618	30208-30209	-LRB-	denotes	[
T8619	30212-30214	CD	denotes	38
T8620	30209-30211	CD	denotes	37
T8621	30211-30212	,	denotes	,
T8622	30214-30215	-RRB-	denotes	]
T8623	30215-30216	.	denotes	.
T8624	30216-30286	sentence	denotes	Thus all of the expansion of tissue sterol pools are as free sterols.
T8625	30217-30221	RB	denotes	Thus
T8626	30266-30269	VBP	denotes	are
T8627	30222-30225	DT	denotes	all
T8628	30226-30228	IN	denotes	of
T8629	30229-30232	DT	denotes	the
T8630	30233-30242	NN	denotes	expansion
T8631	30243-30245	IN	denotes	of
T8632	30246-30252	NN	denotes	tissue
T8633	30260-30265	NNS	denotes	pools
T8634	30253-30259	NN	denotes	sterol
T8635	30270-30272	IN	denotes	as
T8636	30273-30277	JJ	denotes	free
T8637	30278-30285	NNS	denotes	sterols
T8638	30285-30286	.	denotes	.
T8639	30286-30467	sentence	denotes	FPLC analyses for sterols (measured enzymatically and thus reflecting total sterols) and triglycerides were performed on plasma samples from fasted animals fed a regular chow diet.
T8640	30287-30291	NN	denotes	FPLC
T8641	30292-30300	NNS	denotes	analyses
T8642	30395-30404	VBN	denotes	performed
T8643	30301-30304	IN	denotes	for
T8644	30305-30312	NNS	denotes	sterols
T8645	30313-30314	-LRB-	denotes	(
T8646	30314-30322	VBN	denotes	measured
T8647	30323-30336	RB	denotes	enzymatically
T8648	30337-30340	CC	denotes	and
T8649	30341-30345	RB	denotes	thus
T8650	30346-30356	VBG	denotes	reflecting
T8651	30357-30362	JJ	denotes	total
T8652	30363-30370	NNS	denotes	sterols
T8653	30370-30371	-RRB-	denotes	)
T8654	30372-30375	CC	denotes	and
T8655	30376-30389	NNS	denotes	triglycerides
T8656	30390-30394	VBD	denotes	were
T8657	30405-30407	IN	denotes	on
T8658	30408-30414	NN	denotes	plasma
T8659	30415-30422	NNS	denotes	samples
T8660	30423-30427	IN	denotes	from
T8661	30428-30434	VBN	denotes	fasted
T8662	30435-30442	NNS	denotes	animals
T8663	30443-30446	VBN	denotes	fed
T8664	30447-30448	DT	denotes	a
T8665	30462-30466	NN	denotes	diet
T8666	30449-30456	JJ	denotes	regular
T8667	30457-30461	NN	denotes	chow
T8668	30466-30467	.	denotes	.
T8669	30467-30726	sentence	denotes	No significant differences were observed for the sterol profiles (Figure 3a), but surprisingly, the Abcg8-/- knockout mice had a significantly higher triglyceride level in the LDL lipoprotein fractions compared to wild-type and heterozygous mice (Figure 3b).
T8670	30468-30470	DT	denotes	No
T8671	30483-30494	NNS	denotes	differences
T8672	30471-30482	JJ	denotes	significant
T8673	30500-30508	VBN	denotes	observed
T8674	30495-30499	VBD	denotes	were
T8675	30509-30512	IN	denotes	for
T8676	30513-30516	DT	denotes	the
T8677	30524-30532	NNS	denotes	profiles
T8678	30517-30523	NN	denotes	sterol
T8679	30533-30534	-LRB-	denotes	(
T8680	30541-30543	NN	denotes	3a
T8681	30534-30540	NN	denotes	Figure
T8682	30543-30544	-RRB-	denotes	)
T8683	30544-30546	,	denotes	,
T8684	30546-30549	CC	denotes	but
T8685	30550-30562	RB	denotes	surprisingly
T8686	30591-30594	VBD	denotes	had
T8687	30562-30564	,	denotes	,
T8688	30564-30567	DT	denotes	the
T8689	30586-30590	NNS	denotes	mice
T8690	30568-30573	NN	denotes	Abcg8
T8691	30577-30585	NN	denotes	knockout
T8692	30573-30574	SYM	denotes	-
T8693	30574-30575	HYPH	denotes	/
T8694	30575-30576	SYM	denotes	-
T8695	30595-30596	DT	denotes	a
T8696	30631-30636	NN	denotes	level
T8697	30597-30610	RB	denotes	significantly
T8698	30611-30617	JJR	denotes	higher
T8699	30618-30630	NN	denotes	triglyceride
T8700	30637-30639	IN	denotes	in
T8701	30640-30643	DT	denotes	the
T8702	30660-30669	NNS	denotes	fractions
T8703	30644-30647	NN	denotes	LDL
T8704	30648-30659	NN	denotes	lipoprotein
T8705	30670-30678	VBN	denotes	compared
T8706	30679-30681	IN	denotes	to
T8707	30682-30686	JJ	denotes	wild
T8708	30687-30691	NN	denotes	type
T8709	30686-30687	HYPH	denotes	-
T8710	30709-30713	NNS	denotes	mice
T8711	30692-30695	CC	denotes	and
T8712	30696-30708	JJ	denotes	heterozygous
T8713	30714-30715	-LRB-	denotes	(
T8714	30722-30724	NN	denotes	3b
T8715	30715-30721	NN	denotes	Figure
T8716	30724-30725	-RRB-	denotes	)
T8717	30725-30726	.	denotes	.
T8718	30726-30849	sentence	denotes	The size of this peak was variable between different littermate analyses, but is always increased in the knockout animals.
T8719	30727-30730	DT	denotes	The
T8720	30731-30735	NN	denotes	size
T8721	30749-30752	VBD	denotes	was
T8722	30736-30738	IN	denotes	of
T8723	30739-30743	DT	denotes	this
T8724	30744-30748	NN	denotes	peak
T8725	30753-30761	JJ	denotes	variable
T8726	30762-30769	IN	denotes	between
T8727	30770-30779	JJ	denotes	different
T8728	30791-30799	NNS	denotes	analyses
T8729	30780-30790	NN	denotes	littermate
T8730	30799-30801	,	denotes	,
T8731	30801-30804	CC	denotes	but
T8732	30805-30807	VBZ	denotes	is
T8733	30808-30814	RB	denotes	always
T8734	30815-30824	JJ	denotes	increased
T8735	30825-30827	IN	denotes	in
T8736	30828-30831	DT	denotes	the
T8737	30841-30848	NNS	denotes	animals
T8738	30832-30840	NN	denotes	knockout
T8739	30848-30849	.	denotes	.
T8740	30849-31044	sentence	denotes	Total plasma triglyceride levels of the Abcg8-/- mice were slightly higher (79.2 ± 14 mg/dL compared to the wild type 63.4 ± 13 mg/dL and heterozygotes 46.6 ± 12 mg/dL, n = 3 for all genotypes).
T8741	30850-30855	JJ	denotes	Total
T8742	30876-30882	NNS	denotes	levels
T8743	30856-30862	NN	denotes	plasma
T8744	30863-30875	NN	denotes	triglyceride
T8745	30904-30908	VBD	denotes	were
T8746	30883-30885	IN	denotes	of
T8747	30886-30889	DT	denotes	the
T8748	30899-30903	NNS	denotes	mice
T8749	30890-30895	NN	denotes	Abcg8
T8750	30895-30896	SYM	denotes	-
T8751	30896-30897	HYPH	denotes	/
T8752	30897-30898	SYM	denotes	-
T8753	30909-30917	RB	denotes	slightly
T8754	30918-30924	JJR	denotes	higher
T8755	30925-30926	-LRB-	denotes	(
T8756	30936-30938	NN	denotes	mg
T8757	30926-30930	CD	denotes	79.2
T8758	30933-30935	CD	denotes	14
T8759	30931-30932	SYM	denotes	±
T8760	30938-30939	SYM	denotes	/
T8761	30939-30941	NN	denotes	dL
T8762	30942-30950	VBN	denotes	compared
T8763	30951-30953	IN	denotes	to
T8764	30954-30957	DT	denotes	the
T8765	30978-30980	NN	denotes	mg
T8766	30958-30962	JJ	denotes	wild
T8767	30963-30967	NN	denotes	type
T8768	30968-30972	CD	denotes	63.4
T8769	30975-30977	CD	denotes	13
T8770	30973-30974	SYM	denotes	±
T8771	30980-30981	SYM	denotes	/
T8772	30981-30983	NN	denotes	dL
T8773	30984-30987	CC	denotes	and
T8774	30988-31001	NNS	denotes	heterozygotes
T8775	31012-31014	NN	denotes	mg
T8776	31002-31006	CD	denotes	46.6
T8777	31009-31011	CD	denotes	12
T8778	31007-31008	SYM	denotes	±
T8779	31014-31015	SYM	denotes	/
T8780	31015-31017	NN	denotes	dL
T8781	31017-31019	,	denotes	,
T8782	31019-31020	NN	denotes	n
T8783	31023-31024	CD	denotes	3
T8784	31021-31022	SYM	denotes	=
T8785	31025-31028	IN	denotes	for
T8786	31029-31032	DT	denotes	all
T8787	31033-31042	NNS	denotes	genotypes
T8788	31042-31043	-RRB-	denotes	)
T8789	31043-31044	.	denotes	.
T8790	31044-31182	sentence	denotes	Preliminary analyses of proteins in the isolated FPLC fractions did not show any qualitative changes in apolipoproteins B, E, AI, or AII.
T8791	31045-31056	JJ	denotes	Preliminary
T8792	31057-31065	NNS	denotes	analyses
T8793	31117-31121	VB	denotes	show
T8794	31066-31068	IN	denotes	of
T8795	31069-31077	NN	denotes	proteins
T8796	31078-31080	IN	denotes	in
T8797	31081-31084	DT	denotes	the
T8798	31099-31108	NNS	denotes	fractions
T8799	31085-31093	VBN	denotes	isolated
T8800	31094-31098	NN	denotes	FPLC
T8801	31109-31112	VBD	denotes	did
T8802	31113-31116	RB	denotes	not
T8803	31122-31125	DT	denotes	any
T8804	31138-31145	NNS	denotes	changes
T8805	31126-31137	JJ	denotes	qualitative
T8806	31146-31148	IN	denotes	in
T8807	31149-31164	NNS	denotes	apolipoproteins
T8808	31165-31166	NN	denotes	B
T8809	31166-31168	,	denotes	,
T8810	31168-31169	NN	denotes	E
T8811	31169-31171	,	denotes	,
T8812	31171-31173	NN	denotes	AI
T8813	31173-31175	,	denotes	,
T8814	31175-31177	CC	denotes	or
T8815	31178-31181	NN	denotes	AII
T8816	31181-31182	.	denotes	.
T8817	31182-31258	sentence	denotes	The significance of this triglyceride rich peak remains unclear at present.
T8818	31183-31186	DT	denotes	The
T8819	31187-31199	NN	denotes	significance
T8820	31231-31238	VBZ	denotes	remains
T8821	31200-31202	IN	denotes	of
T8822	31203-31207	DT	denotes	this
T8823	31226-31230	NN	denotes	peak
T8824	31208-31220	NN	denotes	triglyceride
T8825	31221-31225	JJ	denotes	rich
T8826	31239-31246	JJ	denotes	unclear
T8827	31247-31249	IN	denotes	at
T8828	31250-31257	JJ	denotes	present
T8829	31257-31258	.	denotes	.
T9063	31260-31265	NN	denotes	Liver
T9064	31271-31281	NN	denotes	expression
T9065	31266-31270	NN	denotes	gene
T9066	31302-31308	VBP	denotes	change
T9067	31282-31285	CC	denotes	and
T9068	31286-31292	NN	denotes	enzyme
T9069	31293-31301	NN	denotes	activity
T9070	31309-31311	IN	denotes	in
T9071	31312-31317	NN	denotes	Abcg8
T9072	31318-31326	NN	denotes	sterolin
T9073	31317-31318	HYPH	denotes	/
T9074	31329-31338	JJ	denotes	deficient
T9075	31326-31327	HYPH	denotes	-
T9076	31327-31328	CD	denotes	2
T9077	31339-31343	NNS	denotes	mice
T9078	31343-31654	sentence	denotes	To investigate the effects of a deficiency of Abcg8/sterolin-2 on the genes that regulate sterol metabolism, quantitative RT-PCR was performed looking at the expression levels of Abcg5, Abcg8, Hmgr, Cyp7a1, Abca1, Mdr2, Lxr, Srebp-1c, and Srebp-2 mRNA in the livers of mice fed a regular chow diet (Figure 4a).
T9079	31344-31346	TO	denotes	To
T9080	31347-31358	VB	denotes	investigate
T9081	31477-31486	VBN	denotes	performed
T9082	31359-31362	DT	denotes	the
T9083	31363-31370	NNS	denotes	effects
T9084	31371-31373	IN	denotes	of
T9085	31374-31375	DT	denotes	a
T9086	31376-31386	NN	denotes	deficiency
T9087	31387-31389	IN	denotes	of
T9088	31390-31395	NN	denotes	Abcg8
T9089	31396-31404	NN	denotes	sterolin
T9090	31395-31396	HYPH	denotes	/
T9091	31404-31405	HYPH	denotes	-
T9092	31405-31406	CD	denotes	2
T9093	31407-31409	IN	denotes	on
T9094	31410-31413	DT	denotes	the
T9095	31414-31419	NNS	denotes	genes
T9096	31420-31424	WDT	denotes	that
T9097	31425-31433	VBP	denotes	regulate
T9098	31434-31440	NN	denotes	sterol
T9099	31441-31451	NN	denotes	metabolism
T9100	31451-31453	,	denotes	,
T9101	31453-31465	JJ	denotes	quantitative
T9102	31469-31472	NN	denotes	PCR
T9103	31466-31468	NN	denotes	RT
T9104	31468-31469	HYPH	denotes	-
T9105	31473-31476	VBD	denotes	was
T9106	31487-31494	VBG	denotes	looking
T9107	31495-31497	IN	denotes	at
T9108	31498-31501	DT	denotes	the
T9109	31513-31519	NNS	denotes	levels
T9110	31502-31512	NN	denotes	expression
T9111	31520-31522	IN	denotes	of
T9112	31523-31528	NN	denotes	Abcg5
T9113	31591-31595	NN	denotes	mRNA
T9114	31528-31530	,	denotes	,
T9115	31530-31535	NN	denotes	Abcg8
T9116	31535-31537	,	denotes	,
T9117	31537-31541	NN	denotes	Hmgr
T9118	31541-31543	,	denotes	,
T9119	31543-31549	NN	denotes	Cyp7a1
T9120	31549-31551	,	denotes	,
T9121	31551-31556	NN	denotes	Abca1
T9122	31556-31558	,	denotes	,
T9123	31558-31562	NN	denotes	Mdr2
T9124	31562-31564	,	denotes	,
T9125	31564-31567	NN	denotes	Lxr
T9126	31567-31569	,	denotes	,
T9127	31569-31574	NN	denotes	Srebp
T9128	31575-31577	NN	denotes	1c
T9129	31574-31575	HYPH	denotes	-
T9130	31577-31579	,	denotes	,
T9131	31579-31582	CC	denotes	and
T9132	31583-31588	NN	denotes	Srebp
T9133	31588-31589	HYPH	denotes	-
T9134	31589-31590	CD	denotes	2
T9135	31596-31598	IN	denotes	in
T9136	31599-31602	DT	denotes	the
T9137	31603-31609	NNS	denotes	livers
T9138	31610-31612	IN	denotes	of
T9139	31613-31617	NNS	denotes	mice
T9140	31618-31621	VBN	denotes	fed
T9141	31622-31623	DT	denotes	a
T9142	31637-31641	NN	denotes	diet
T9143	31624-31631	JJ	denotes	regular
T9144	31632-31636	NN	denotes	chow
T9145	31642-31643	-LRB-	denotes	(
T9146	31650-31652	NN	denotes	4a
T9147	31643-31649	NN	denotes	Figure
T9148	31652-31653	-RRB-	denotes	)
T9149	31653-31654	.	denotes	.
T9150	31654-31814	sentence	denotes	As expected, Abcg8 mRNA expression levels were undetectable in the Abcg8-/- mice and were reduced by ~50% in the heterozygous mice, relative to wild-type mice.
T9151	31655-31657	IN	denotes	As
T9152	31658-31666	VBN	denotes	expected
T9153	31697-31701	VBD	denotes	were
T9154	31666-31668	,	denotes	,
T9155	31668-31673	NN	denotes	Abcg8
T9156	31674-31678	NN	denotes	mRNA
T9157	31690-31696	NNS	denotes	levels
T9158	31679-31689	NN	denotes	expression
T9159	31702-31714	JJ	denotes	undetectable
T9160	31715-31717	IN	denotes	in
T9161	31718-31721	DT	denotes	the
T9162	31731-31735	NNS	denotes	mice
T9163	31722-31727	NN	denotes	Abcg8
T9164	31727-31728	SYM	denotes	-
T9165	31728-31729	HYPH	denotes	/
T9166	31729-31730	SYM	denotes	-
T9167	31736-31739	CC	denotes	and
T9168	31740-31744	VBD	denotes	were
T9169	31745-31752	VBN	denotes	reduced
T9170	31753-31755	IN	denotes	by
T9171	31756-31757	SYM	denotes	~
T9172	31757-31759	CD	denotes	50
T9173	31759-31760	NN	denotes	%
T9174	31761-31763	IN	denotes	in
T9175	31764-31767	DT	denotes	the
T9176	31781-31785	NNS	denotes	mice
T9177	31768-31780	JJ	denotes	heterozygous
T9178	31785-31787	,	denotes	,
T9179	31787-31795	JJ	denotes	relative
T9180	31796-31798	IN	denotes	to
T9181	31799-31803	JJ	denotes	wild
T9182	31804-31808	NN	denotes	type
T9183	31803-31804	HYPH	denotes	-
T9184	31809-31813	NNS	denotes	mice
T9185	31813-31814	.	denotes	.
T9186	31814-32027	sentence	denotes	Interestingly, by quantitative RT-PCR, the mRNA expression of Abcg5 in the knock-out mice was also reduced by more than 60% compared to the wild-type mice, although no changes were noted in the heterozygous mice.
T9187	31815-31828	RB	denotes	Interestingly
T9188	31914-31921	VBN	denotes	reduced
T9189	31828-31830	,	denotes	,
T9190	31830-31832	IN	denotes	by
T9191	31833-31845	JJ	denotes	quantitative
T9192	31849-31852	NN	denotes	PCR
T9193	31846-31848	NN	denotes	RT
T9194	31848-31849	HYPH	denotes	-
T9195	31852-31854	,	denotes	,
T9196	31854-31857	DT	denotes	the
T9197	31863-31873	NN	denotes	expression
T9198	31858-31862	NN	denotes	mRNA
T9199	31874-31876	IN	denotes	of
T9200	31877-31882	NN	denotes	Abcg5
T9201	31883-31885	IN	denotes	in
T9202	31886-31889	DT	denotes	the
T9203	31900-31904	NNS	denotes	mice
T9204	31890-31895	VB	denotes	knock
T9205	31895-31896	HYPH	denotes	-
T9206	31896-31899	RP	denotes	out
T9207	31905-31908	VBD	denotes	was
T9208	31909-31913	RB	denotes	also
T9209	31922-31924	IN	denotes	by
T9210	31925-31929	JJR	denotes	more
T9211	31935-31937	CD	denotes	60
T9212	31930-31934	IN	denotes	than
T9213	31937-31938	NN	denotes	%
T9214	31939-31947	VBN	denotes	compared
T9215	31948-31950	IN	denotes	to
T9216	31951-31954	DT	denotes	the
T9217	31965-31969	NNS	denotes	mice
T9218	31955-31959	JJ	denotes	wild
T9219	31960-31964	NN	denotes	type
T9220	31959-31960	HYPH	denotes	-
T9221	31969-31971	,	denotes	,
T9222	31971-31979	IN	denotes	although
T9223	31996-32001	VBN	denotes	noted
T9224	31980-31982	DT	denotes	no
T9225	31983-31990	NNS	denotes	changes
T9226	31991-31995	VBD	denotes	were
T9227	32002-32004	IN	denotes	in
T9228	32005-32008	DT	denotes	the
T9229	32022-32026	NNS	denotes	mice
T9230	32009-32021	JJ	denotes	heterozygous
T9231	32026-32027	.	denotes	.
T9232	32027-32226	sentence	denotes	Expression of HMG-CoA reductase mRNA was decreased by ~50% and ~80% in the heterozygote and knockout mice respectively, in keeping with limited observations in human patients with this disorder [7].
T9233	32028-32038	NN	denotes	Expression
T9234	32069-32078	VBN	denotes	decreased
T9235	32039-32041	IN	denotes	of
T9236	32042-32045	NN	denotes	HMG
T9237	32046-32049	NN	denotes	CoA
T9238	32045-32046	HYPH	denotes	-
T9239	32050-32059	NN	denotes	reductase
T9240	32060-32064	NN	denotes	mRNA
T9241	32065-32068	VBD	denotes	was
T9242	32079-32081	IN	denotes	by
T9243	32082-32083	SYM	denotes	~
T9244	32083-32085	CD	denotes	50
T9245	32085-32086	NN	denotes	%
T9246	32087-32090	CC	denotes	and
T9247	32091-32092	SYM	denotes	~
T9248	32092-32094	CD	denotes	80
T9249	32094-32095	NN	denotes	%
T9250	32096-32098	IN	denotes	in
T9251	32099-32102	DT	denotes	the
T9252	32129-32133	NNS	denotes	mice
T9253	32103-32115	NN	denotes	heterozygote
T9254	32116-32119	CC	denotes	and
T9255	32120-32128	NN	denotes	knockout
T9256	32134-32146	RB	denotes	respectively
T9257	32146-32148	,	denotes	,
T9258	32148-32150	IN	denotes	in
T9259	32151-32158	VBG	denotes	keeping
T9260	32159-32163	IN	denotes	with
T9261	32164-32171	JJ	denotes	limited
T9262	32172-32184	NNS	denotes	observations
T9263	32185-32187	IN	denotes	in
T9264	32188-32193	JJ	denotes	human
T9265	32194-32202	NNS	denotes	patients
T9266	32203-32207	IN	denotes	with
T9267	32208-32212	DT	denotes	this
T9268	32213-32221	NN	denotes	disorder
T9269	32222-32223	-LRB-	denotes	[
T9270	32223-32224	CD	denotes	7
T9271	32224-32225	-RRB-	denotes	]
T9272	32225-32226	.	denotes	.
T9273	32226-32408	sentence	denotes	To verify whether the mRNA changes resulted in alteration of the enzyme activity changes, liver samples were analyzed for HMG-CoA reductase activity and Cyp7a1 activity (Figure 4b).
T9274	32227-32229	TO	denotes	To
T9275	32230-32236	VB	denotes	verify
T9276	32336-32344	VBN	denotes	analyzed
T9277	32237-32244	IN	denotes	whether
T9278	32262-32270	VBD	denotes	resulted
T9279	32245-32248	DT	denotes	the
T9280	32254-32261	NNS	denotes	changes
T9281	32249-32253	NN	denotes	mRNA
T9282	32271-32273	IN	denotes	in
T9283	32274-32284	NN	denotes	alteration
T9284	32285-32287	IN	denotes	of
T9285	32288-32291	DT	denotes	the
T9286	32308-32315	NNS	denotes	changes
T9287	32292-32298	NN	denotes	enzyme
T9288	32299-32307	NN	denotes	activity
T9289	32315-32317	,	denotes	,
T9290	32317-32322	NN	denotes	liver
T9291	32323-32330	NNS	denotes	samples
T9292	32331-32335	VBD	denotes	were
T9293	32345-32348	IN	denotes	for
T9294	32349-32352	NN	denotes	HMG
T9295	32353-32356	NN	denotes	CoA
T9296	32352-32353	HYPH	denotes	-
T9297	32357-32366	NN	denotes	reductase
T9298	32367-32375	NN	denotes	activity
T9299	32376-32379	CC	denotes	and
T9300	32380-32386	NN	denotes	Cyp7a1
T9301	32387-32395	NN	denotes	activity
T9302	32396-32397	-LRB-	denotes	(
T9303	32404-32406	NN	denotes	4b
T9304	32397-32403	NN	denotes	Figure
T9305	32406-32407	-RRB-	denotes	)
T9306	32407-32408	.	denotes	.
T9307	32408-32574	sentence	denotes	HMG-CoA reductase activity was reduced by 30% and 60% in the Abcg8+/- and Abcg8-/- mice, respectively (Figure 4b), and thus reflected the changes in mRNA expression.
T9308	32409-32412	NN	denotes	HMG
T9309	32413-32416	NN	denotes	CoA
T9310	32412-32413	HYPH	denotes	-
T9311	32417-32426	NN	denotes	reductase
T9312	32427-32435	NN	denotes	activity
T9313	32440-32447	VBN	denotes	reduced
T9314	32436-32439	VBD	denotes	was
T9315	32448-32450	IN	denotes	by
T9316	32451-32453	CD	denotes	30
T9317	32453-32454	NN	denotes	%
T9318	32455-32458	CC	denotes	and
T9319	32459-32461	CD	denotes	60
T9320	32461-32462	NN	denotes	%
T9321	32463-32465	IN	denotes	in
T9322	32466-32469	DT	denotes	the
T9323	32492-32496	NNS	denotes	mice
T9324	32470-32475	NN	denotes	Abcg8
T9325	32475-32476	SYM	denotes	+
T9326	32476-32477	HYPH	denotes	/
T9327	32477-32478	SYM	denotes	-
T9328	32479-32482	CC	denotes	and
T9329	32483-32488	NN	denotes	Abcg8
T9330	32488-32489	SYM	denotes	-
T9331	32489-32490	HYPH	denotes	/
T9332	32490-32491	SYM	denotes	-
T9333	32496-32498	,	denotes	,
T9334	32498-32510	RB	denotes	respectively
T9335	32511-32512	-LRB-	denotes	(
T9336	32519-32521	NN	denotes	4b
T9337	32512-32518	NN	denotes	Figure
T9338	32521-32522	-RRB-	denotes	)
T9339	32522-32524	,	denotes	,
T9340	32524-32527	CC	denotes	and
T9341	32528-32532	RB	denotes	thus
T9342	32533-32542	VBD	denotes	reflected
T9343	32543-32546	DT	denotes	the
T9344	32547-32554	NNS	denotes	changes
T9345	32555-32557	IN	denotes	in
T9346	32558-32562	NN	denotes	mRNA
T9347	32563-32573	NN	denotes	expression
T9348	32573-32574	.	denotes	.
T9349	32574-32743	sentence	denotes	In contrast, although the Cyp7a1 mRNA expression levels were essentially unchanged in the knockout mouse, Cyp7a1 activity was significantly decreased by 37% (P < 0.01).
T9350	32575-32577	IN	denotes	In
T9351	32715-32724	VBN	denotes	decreased
T9352	32578-32586	NN	denotes	contrast
T9353	32586-32588	,	denotes	,
T9354	32588-32596	IN	denotes	although
T9355	32631-32635	VBD	denotes	were
T9356	32597-32600	DT	denotes	the
T9357	32624-32630	NNS	denotes	levels
T9358	32601-32607	NN	denotes	Cyp7a1
T9359	32608-32612	NN	denotes	mRNA
T9360	32613-32623	NN	denotes	expression
T9361	32636-32647	RB	denotes	essentially
T9362	32648-32657	JJ	denotes	unchanged
T9363	32658-32660	IN	denotes	in
T9364	32661-32664	DT	denotes	the
T9365	32674-32679	NN	denotes	mouse
T9366	32665-32673	NN	denotes	knockout
T9367	32679-32681	,	denotes	,
T9368	32681-32687	NN	denotes	Cyp7a1
T9369	32688-32696	NN	denotes	activity
T9370	32697-32700	VBD	denotes	was
T9371	32701-32714	RB	denotes	significantly
T9372	32725-32727	IN	denotes	by
T9373	32728-32730	CD	denotes	37
T9374	32730-32731	NN	denotes	%
T9375	32732-32733	-LRB-	denotes	(
T9376	32737-32741	CD	denotes	0.01
T9377	32733-32734	NN	denotes	P
T9378	32735-32736	SYM	denotes	<
T9379	32741-32742	-RRB-	denotes	)
T9380	32742-32743	.	denotes	.
T9381	32743-32822	sentence	denotes	In the heterozygous mice, both the mRNA and activity of Cyp7a1 were decreased.
T9382	32744-32746	IN	denotes	In
T9383	32812-32821	VBN	denotes	decreased
T9384	32747-32750	DT	denotes	the
T9385	32764-32768	NNS	denotes	mice
T9386	32751-32763	JJ	denotes	heterozygous
T9387	32768-32770	,	denotes	,
T9388	32770-32774	CC	denotes	both
T9389	32779-32783	NN	denotes	mRNA
T9390	32775-32778	DT	denotes	the
T9391	32784-32787	CC	denotes	and
T9392	32788-32796	NN	denotes	activity
T9393	32797-32799	IN	denotes	of
T9394	32800-32806	NN	denotes	Cyp7a1
T9395	32807-32811	VBD	denotes	were
T9396	32821-32822	.	denotes	.
T9397	32822-33014	sentence	denotes	Sitosterol is known to be a direct competitive inhibitor of Cyp7a1 and it is likely that the elevated plant sterols in the liver are responsible for the inhibition in the knockout mouse [39].
T9398	32823-32833	NN	denotes	Sitosterol
T9399	32837-32842	VBN	denotes	known
T9400	32834-32836	VBZ	denotes	is
T9401	32843-32845	TO	denotes	to
T9402	32846-32848	VB	denotes	be
T9403	32849-32850	DT	denotes	a
T9404	32870-32879	NN	denotes	inhibitor
T9405	32851-32857	JJ	denotes	direct
T9406	32858-32869	JJ	denotes	competitive
T9407	32880-32882	IN	denotes	of
T9408	32883-32889	NN	denotes	Cyp7a1
T9409	32890-32893	CC	denotes	and
T9410	32894-32896	PRP	denotes	it
T9411	32897-32899	VBZ	denotes	is
T9412	32900-32906	JJ	denotes	likely
T9413	32907-32911	IN	denotes	that
T9414	32952-32955	VBP	denotes	are
T9415	32912-32915	DT	denotes	the
T9416	32931-32938	NNS	denotes	sterols
T9417	32916-32924	JJ	denotes	elevated
T9418	32925-32930	NN	denotes	plant
T9419	32939-32941	IN	denotes	in
T9420	32942-32945	DT	denotes	the
T9421	32946-32951	NN	denotes	liver
T9422	32956-32967	JJ	denotes	responsible
T9423	32968-32971	IN	denotes	for
T9424	32972-32975	DT	denotes	the
T9425	32976-32986	NN	denotes	inhibition
T9426	32987-32989	IN	denotes	in
T9427	32990-32993	DT	denotes	the
T9428	33003-33008	NN	denotes	mouse
T9429	32994-33002	NN	denotes	knockout
T9430	33009-33010	-LRB-	denotes	[
T9431	33010-33012	CD	denotes	39
T9432	33012-33013	-RRB-	denotes	]
T9433	33013-33014	.	denotes	.
T9860	33016-33028	NN	denotes	Localization
T9861	33029-33031	IN	denotes	of
T9862	33032-33037	NN	denotes	Abcg5
T9863	33038-33045	NN	denotes	protein
T9864	33046-33048	IN	denotes	in
T9865	33049-33054	NN	denotes	Abcg8
T9866	33058-33066	NN	denotes	knockout
T9867	33054-33055	SYM	denotes	-
T9868	33055-33056	HYPH	denotes	/
T9869	33056-33057	SYM	denotes	-
T9870	33067-33071	NNS	denotes	mice
T9871	33071-33267	sentence	denotes	Does the loss of Abcg8/sterolin-2 result in loss of Abcg5/sterolin-1 expression, as might be predicted from the genetic studies and more recently from the in vitro and in vivo expression studies?
T9872	33072-33076	VBZ	denotes	Does
T9873	33106-33112	VB	denotes	result
T9874	33077-33080	DT	denotes	the
T9875	33081-33085	NN	denotes	loss
T9876	33086-33088	IN	denotes	of
T9877	33089-33094	NN	denotes	Abcg8
T9878	33095-33103	NN	denotes	sterolin
T9879	33094-33095	HYPH	denotes	/
T9880	33103-33104	HYPH	denotes	-
T9881	33104-33105	CD	denotes	2
T9882	33113-33115	IN	denotes	in
T9883	33116-33120	NN	denotes	loss
T9884	33121-33123	IN	denotes	of
T9885	33124-33129	NN	denotes	Abcg5
T9886	33130-33138	NN	denotes	sterolin
T9887	33129-33130	HYPH	denotes	/
T9888	33141-33151	NN	denotes	expression
T9889	33138-33139	HYPH	denotes	-
T9890	33139-33140	CD	denotes	1
T9891	33151-33153	,	denotes	,
T9892	33153-33155	IN	denotes	as
T9893	33165-33174	VBN	denotes	predicted
T9894	33156-33161	MD	denotes	might
T9895	33162-33164	VB	denotes	be
T9896	33175-33179	IN	denotes	from
T9897	33180-33183	DT	denotes	the
T9898	33192-33199	NNS	denotes	studies
T9899	33184-33191	JJ	denotes	genetic
T9900	33200-33203	CC	denotes	and
T9901	33204-33208	RBR	denotes	more
T9902	33209-33217	RB	denotes	recently
T9903	33218-33222	IN	denotes	from
T9904	33223-33226	DT	denotes	the
T9905	33259-33266	NNS	denotes	studies
T9906	33227-33229	FW	denotes	in
T9907	33230-33235	FW	denotes	vitro
T9908	33236-33239	CC	denotes	and
T9909	33240-33242	FW	denotes	in
T9910	33243-33247	FW	denotes	vivo
T9911	33248-33258	NN	denotes	expression
T9912	33266-33267	.	denotes	?
T9913	33267-33339	sentence	denotes	A robust antibody to mouse Abcg8/sterolin-2 is not currently available.
T9914	33268-33269	DT	denotes	A
T9915	33277-33285	NN	denotes	antibody
T9916	33270-33276	JJ	denotes	robust
T9917	33312-33314	VBZ	denotes	is
T9918	33286-33288	IN	denotes	to
T9919	33289-33294	NN	denotes	mouse
T9920	33301-33309	NN	denotes	sterolin
T9921	33295-33300	NN	denotes	Abcg8
T9922	33300-33301	HYPH	denotes	/
T9923	33309-33310	HYPH	denotes	-
T9924	33310-33311	CD	denotes	2
T9925	33315-33318	RB	denotes	not
T9926	33319-33328	RB	denotes	currently
T9927	33329-33338	JJ	denotes	available
T9928	33338-33339	.	denotes	.
T9929	33339-33439	sentence	denotes	However, three separate groups have developed rabbit polyclonal antibody to mouse Abcg5/sterolin-1.
T9930	33340-33347	RB	denotes	However
T9931	33376-33385	VBN	denotes	developed
T9932	33347-33349	,	denotes	,
T9933	33349-33354	CD	denotes	three
T9934	33364-33370	NNS	denotes	groups
T9935	33355-33363	JJ	denotes	separate
T9936	33371-33375	VBP	denotes	have
T9937	33386-33392	NN	denotes	rabbit
T9938	33404-33412	NN	denotes	antibody
T9939	33393-33403	JJ	denotes	polyclonal
T9940	33413-33415	IN	denotes	to
T9941	33416-33421	NN	denotes	mouse
T9942	33428-33436	NN	denotes	sterolin
T9943	33422-33427	NN	denotes	Abcg5
T9944	33427-33428	HYPH	denotes	/
T9945	33436-33437	HYPH	denotes	-
T9946	33437-33438	CD	denotes	1
T9947	33438-33439	.	denotes	.
T9948	33439-33574	sentence	denotes	These three antibodies were used in Western blotting and immunohistochemistry experiments on the liver and intestine of Abcg8-/- mice.
T9949	33440-33445	DT	denotes	These
T9950	33452-33462	NNS	denotes	antibodies
T9951	33446-33451	CD	denotes	three
T9952	33468-33472	VBN	denotes	used
T9954	33473-33475	IN	denotes	in
T9955	33476-33483	NNP	denotes	Western
T9956	33484-33492	NN	denotes	blotting
T9957	33493-33496	CC	denotes	and
T9958	33497-33517	NN	denotes	immunohistochemistry
T9959	33518-33529	NNS	denotes	experiments
T9960	33530-33532	IN	denotes	on
T9961	33533-33536	DT	denotes	the
T9962	33537-33542	NN	denotes	liver
T9963	33543-33546	CC	denotes	and
T9964	33547-33556	NN	denotes	intestine
T9965	33557-33559	IN	denotes	of
T9966	33560-33565	NN	denotes	Abcg8
T9967	33569-33573	NNS	denotes	mice
T9968	33565-33566	SYM	denotes	-
T9969	33566-33567	HYPH	denotes	/
T9970	33567-33568	SYM	denotes	-
T9971	33573-33574	.	denotes	.
T9972	33574-33724	sentence	denotes	Western blotting of liver total membrane preparations using the three separately-developed anti-Abcg5/sterolin-1 antibodies showed different results.
T9973	33575-33582	NNP	denotes	Western
T9974	33583-33591	NN	denotes	blotting
T9975	33699-33705	VBD	denotes	showed
T9976	33592-33594	IN	denotes	of
T9977	33595-33600	NN	denotes	liver
T9978	33616-33628	NNS	denotes	preparations
T9979	33601-33606	JJ	denotes	total
T9980	33607-33615	NN	denotes	membrane
T9981	33629-33634	VBG	denotes	using
T9982	33635-33638	DT	denotes	the
T9983	33688-33698	NNS	denotes	antibodies
T9984	33639-33644	CD	denotes	three
T9985	33645-33655	RB	denotes	separately
T9986	33656-33665	VBN	denotes	developed
T9987	33655-33656	HYPH	denotes	-
T9988	33666-33676	NN	denotes	anti-Abcg5
T9989	33677-33685	NN	denotes	sterolin
T9990	33676-33677	HYPH	denotes	/
T9991	33685-33686	HYPH	denotes	-
T9992	33686-33687	CD	denotes	1
T9993	33706-33715	JJ	denotes	different
T9994	33716-33723	NNS	denotes	results
T9995	33723-33724	.	denotes	.
T9996	33724-33893	sentence	denotes	As has been previously published, Abcg5/sterolin-1 exists in two separate forms, the 'immature' 75 kDa protein and a fully glycosylated 'mature' 93 kDa protein [18,30].
T9997	33725-33727	IN	denotes	As
T9998	33748-33757	VBN	denotes	published
T9999	33728-33731	VBZ	denotes	has
T10000	33732-33736	VBN	denotes	been
T10001	33737-33747	RB	denotes	previously
T10002	33776-33782	VBZ	denotes	exists
T10003	33757-33759	,	denotes	,
T10004	33759-33764	NN	denotes	Abcg5
T10005	33765-33773	NN	denotes	sterolin
T10006	33764-33765	HYPH	denotes	/
T10007	33773-33774	HYPH	denotes	-
T10008	33774-33775	CD	denotes	1
T10009	33783-33785	IN	denotes	in
T10010	33786-33789	CD	denotes	two
T10011	33799-33804	NNS	denotes	forms
T10012	33790-33798	JJ	denotes	separate
T10013	33804-33806	,	denotes	,
T10014	33806-33809	DT	denotes	the
T10015	33828-33835	NN	denotes	protein
T10016	33810-33811	``	denotes	'
T10017	33811-33819	NN	denotes	immature
T10018	33819-33820	''	denotes	'
T10019	33821-33823	CD	denotes	75
T10020	33824-33827	NN	denotes	kDa
T10021	33836-33839	CC	denotes	and
T10022	33840-33841	DT	denotes	a
T10023	33877-33884	NN	denotes	protein
T10024	33842-33847	RB	denotes	fully
T10025	33848-33860	VBN	denotes	glycosylated
T10026	33861-33862	``	denotes	'
T10027	33862-33868	NN	denotes	mature
T10028	33868-33869	''	denotes	'
T10029	33870-33872	CD	denotes	93
T10030	33873-33876	NN	denotes	kDa
T10031	33885-33886	-LRB-	denotes	[
T10032	33889-33891	CD	denotes	30
T10033	33886-33888	CD	denotes	18
T10034	33888-33889	,	denotes	,
T10035	33891-33892	-RRB-	denotes	]
T10036	33892-33893	.	denotes	.
T10037	33893-33985	sentence	denotes	Using the SC anti-Abcg5/sterolin-1 peptide antibody, a 75 kDa band is detected (Figure 5a).
T10038	33894-33899	VBG	denotes	Using
T10039	33964-33972	VBN	denotes	detected
T10040	33900-33903	DT	denotes	the
T10041	33937-33945	NN	denotes	antibody
T10042	33904-33906	NN	denotes	SC
T10043	33929-33936	NN	denotes	peptide
T10044	33907-33917	NN	denotes	anti-Abcg5
T10045	33918-33926	NN	denotes	sterolin
T10046	33917-33918	HYPH	denotes	/
T10047	33926-33927	HYPH	denotes	-
T10048	33927-33928	CD	denotes	1
T10049	33945-33947	,	denotes	,
T10050	33947-33948	DT	denotes	a
T10051	33956-33960	NN	denotes	band
T10052	33949-33951	CD	denotes	75
T10053	33952-33955	NN	denotes	kDa
T10054	33961-33963	VBZ	denotes	is
T10055	33973-33974	-LRB-	denotes	(
T10056	33981-33983	NN	denotes	5a
T10057	33974-33980	NN	denotes	Figure
T10058	33983-33984	-RRB-	denotes	)
T10059	33984-33985	.	denotes	.
T10060	33985-34203	sentence	denotes	This antibody does not detect a 'mature' 93 kDa band in either wild-type or knockout animals nor is the 75 kDa band sensitive to either Endo-H or PNGaseF (lower panel shows same aliquots probed with anti-transferrin).
T10061	33986-33990	DT	denotes	This
T10062	33991-33999	NN	denotes	antibody
T10063	34009-34015	VB	denotes	detect
T10064	34000-34004	VBZ	denotes	does
T10065	34005-34008	RB	denotes	not
T10066	34016-34017	DT	denotes	a
T10067	34034-34038	NN	denotes	band
T10068	34018-34019	``	denotes	'
T10069	34019-34025	JJ	denotes	mature
T10070	34025-34026	''	denotes	'
T10071	34027-34029	CD	denotes	93
T10072	34030-34033	NN	denotes	kDa
T10073	34039-34041	IN	denotes	in
T10074	34042-34048	CC	denotes	either
T10075	34071-34078	NNS	denotes	animals
T10076	34049-34053	JJ	denotes	wild
T10077	34054-34058	NN	denotes	type
T10078	34053-34054	HYPH	denotes	-
T10079	34059-34061	CC	denotes	or
T10080	34062-34070	NN	denotes	knockout
T10081	34079-34082	CC	denotes	nor
T10082	34083-34085	VBZ	denotes	is
T10083	34086-34089	DT	denotes	the
T10084	34097-34101	NN	denotes	band
T10085	34090-34092	CD	denotes	75
T10086	34093-34096	NN	denotes	kDa
T10087	34102-34111	JJ	denotes	sensitive
T10088	34112-34114	IN	denotes	to
T10089	34115-34121	CC	denotes	either
T10090	34127-34128	NN	denotes	H
T10091	34122-34126	NN	denotes	Endo
T10092	34126-34127	HYPH	denotes	-
T10093	34129-34131	CC	denotes	or
T10094	34132-34139	NN	denotes	PNGaseF
T10095	34140-34141	-LRB-	denotes	(
T10096	34153-34158	VBZ	denotes	shows
T10097	34141-34146	JJ	denotes	lower
T10098	34147-34152	NN	denotes	panel
T10099	34159-34163	JJ	denotes	same
T10100	34164-34172	NNS	denotes	aliquots
T10101	34173-34179	VBN	denotes	probed
T10102	34180-34184	IN	denotes	with
T10103	34185-34201	NN	denotes	anti-transferrin
T10104	34201-34202	-RRB-	denotes	)
T10105	34202-34203	.	denotes	.
T10106	34203-34310	sentence	denotes	The AMC antibody detects the 'mature' form of Abcg5/sterolin-1 in wild-type animals but not the knockouts.
T10107	34204-34207	DT	denotes	The
T10108	34212-34220	NN	denotes	antibody
T10109	34208-34211	NN	denotes	AMC
T10110	34221-34228	VBZ	denotes	detects
T10111	34229-34232	DT	denotes	the
T10112	34242-34246	NN	denotes	form
T10113	34233-34234	``	denotes	'
T10114	34234-34240	NN	denotes	mature
T10115	34240-34241	''	denotes	'
T10116	34247-34249	IN	denotes	of
T10117	34250-34255	NN	denotes	Abcg5
T10118	34256-34264	NN	denotes	sterolin
T10119	34255-34256	HYPH	denotes	/
T10120	34264-34265	HYPH	denotes	-
T10121	34265-34266	CD	denotes	1
T10122	34267-34269	IN	denotes	in
T10123	34270-34274	JJ	denotes	wild
T10124	34275-34279	NN	denotes	type
T10125	34274-34275	HYPH	denotes	-
T10126	34280-34287	NNS	denotes	animals
T10127	34288-34291	CC	denotes	but
T10128	34292-34295	RB	denotes	not
T10129	34296-34299	DT	denotes	the
T10130	34300-34309	NNS	denotes	knockouts
T10131	34309-34310	.	denotes	.
T10132	34310-34449	sentence	denotes	Interestingly, the 'immature' 75 kDa band is detected by this antibody but the band shifts with Endo-H and PNGase F treatment (Figure 5b).
T10133	34311-34324	RB	denotes	Interestingly
T10134	34356-34364	VBN	denotes	detected
T10135	34324-34326	,	denotes	,
T10136	34326-34329	DT	denotes	the
T10137	34348-34352	NN	denotes	band
T10138	34330-34331	``	denotes	'
T10139	34331-34339	JJ	denotes	immature
T10140	34339-34340	''	denotes	'
T10141	34341-34343	CD	denotes	75
T10142	34344-34347	NN	denotes	kDa
T10143	34353-34355	VBZ	denotes	is
T10144	34365-34367	IN	denotes	by
T10145	34368-34372	DT	denotes	this
T10146	34373-34381	NN	denotes	antibody
T10147	34382-34385	CC	denotes	but
T10148	34386-34389	DT	denotes	the
T10149	34390-34394	NN	denotes	band
T10150	34395-34401	VBZ	denotes	shifts
T10151	34402-34406	IN	denotes	with
T10152	34407-34411	NN	denotes	Endo
T10153	34412-34413	NN	denotes	H
T10154	34411-34412	HYPH	denotes	-
T10155	34427-34436	NN	denotes	treatment
T10156	34414-34417	CC	denotes	and
T10157	34418-34424	NN	denotes	PNGase
T10158	34425-34426	NN	denotes	F
T10159	34437-34438	-LRB-	denotes	(
T10160	34445-34447	NN	denotes	5b
T10161	34438-34444	NN	denotes	Figure
T10162	34447-34448	-RRB-	denotes	)
T10163	34448-34449	.	denotes	.
T10164	34449-34606	sentence	denotes	The UTSW antibody detects the 'mature' and 'immature' forms of Abcg5/sterolin-1 in wild-type animals but detects no forms in the knockout mouse (Figure 5c).
T10165	34450-34453	DT	denotes	The
T10166	34459-34467	NN	denotes	antibody
T10167	34454-34458	NN	denotes	UTSW
T10168	34468-34475	VBZ	denotes	detects
T10169	34476-34479	DT	denotes	the
T10170	34504-34509	NNS	denotes	forms
T10171	34480-34481	``	denotes	'
T10172	34481-34487	JJ	denotes	mature
T10173	34487-34488	''	denotes	'
T10174	34489-34492	CC	denotes	and
T10175	34493-34494	``	denotes	'
T10176	34494-34502	JJ	denotes	immature
T10177	34502-34503	''	denotes	'
T10178	34510-34512	IN	denotes	of
T10179	34513-34518	NN	denotes	Abcg5
T10180	34519-34527	NN	denotes	sterolin
T10181	34518-34519	HYPH	denotes	/
T10182	34527-34528	HYPH	denotes	-
T10183	34528-34529	CD	denotes	1
T10184	34530-34532	IN	denotes	in
T10185	34533-34537	JJ	denotes	wild
T10186	34538-34542	NN	denotes	type
T10187	34537-34538	HYPH	denotes	-
T10188	34543-34550	NNS	denotes	animals
T10189	34551-34554	CC	denotes	but
T10190	34555-34562	VBZ	denotes	detects
T10191	34563-34565	DT	denotes	no
T10192	34566-34571	NNS	denotes	forms
T10193	34572-34574	IN	denotes	in
T10194	34575-34578	DT	denotes	the
T10195	34588-34593	NN	denotes	mouse
T10196	34579-34587	NN	denotes	knockout
T10197	34594-34595	-LRB-	denotes	(
T10198	34602-34604	NN	denotes	5c
T10199	34595-34601	NN	denotes	Figure
T10200	34604-34605	-RRB-	denotes	)
T10201	34605-34606	.	denotes	.
T10202	34606-34710	sentence	denotes	What does hold true for all antibodies used against Abcg5/sterolin-1 is the detection of a 75 kDa band.
T10203	34607-34611	WP	denotes	What
T10204	34617-34621	VB	denotes	hold
T10205	34612-34616	VBZ	denotes	does
T10206	34676-34678	VBZ	denotes	is
T10207	34622-34626	JJ	denotes	true
T10208	34627-34630	IN	denotes	for
T10209	34631-34634	DT	denotes	all
T10210	34635-34645	NNS	denotes	antibodies
T10211	34646-34650	VBN	denotes	used
T10212	34651-34658	IN	denotes	against
T10213	34659-34664	NN	denotes	Abcg5
T10214	34665-34673	NN	denotes	sterolin
T10215	34664-34665	HYPH	denotes	/
T10216	34673-34674	HYPH	denotes	-
T10217	34674-34675	CD	denotes	1
T10218	34679-34682	DT	denotes	the
T10219	34683-34692	NN	denotes	detection
T10220	34693-34695	IN	denotes	of
T10221	34696-34697	DT	denotes	a
T10222	34705-34709	NN	denotes	band
T10223	34698-34700	CD	denotes	75
T10224	34701-34704	NN	denotes	kDa
T10225	34709-34710	.	denotes	.
T10226	34710-34788	sentence	denotes	Immunohistochemistry experiments were carried out using all three antibodies.
T10227	34711-34731	NN	denotes	Immunohistochemistry
T10228	34732-34743	NNS	denotes	experiments
T10229	34749-34756	VBN	denotes	carried
T10230	34744-34748	VBD	denotes	were
T10231	34757-34760	RP	denotes	out
T10232	34761-34766	VBG	denotes	using
T10233	34767-34770	DT	denotes	all
T10234	34777-34787	NNS	denotes	antibodies
T10235	34771-34776	CD	denotes	three
T10236	34787-34788	.	denotes	.
T10237	34788-34947	sentence	denotes	In acute transfection studies, the SC antibody recognized over-expressed mouse Abcg5/sterolin-1 in COS-1 cells, but not mouse Abcg8/sterolin-2 (Figure 6b,6c).
T10238	34789-34791	IN	denotes	In
T10239	34836-34846	VBD	denotes	recognized
T10240	34792-34797	JJ	denotes	acute
T10241	34811-34818	NNS	denotes	studies
T10242	34798-34810	NN	denotes	transfection
T10243	34818-34820	,	denotes	,
T10244	34820-34823	DT	denotes	the
T10245	34827-34835	NN	denotes	antibody
T10246	34824-34826	NN	denotes	SC
T10247	34847-34861	VBN	denotes	over-expressed
T10248	34874-34882	NN	denotes	sterolin
T10249	34862-34867	NN	denotes	mouse
T10250	34868-34873	NN	denotes	Abcg5
T10251	34873-34874	HYPH	denotes	/
T10252	34882-34883	HYPH	denotes	-
T10253	34883-34884	CD	denotes	1
T10254	34885-34887	IN	denotes	in
T10255	34888-34891	NN	denotes	COS
T10256	34894-34899	NNS	denotes	cells
T10257	34891-34892	HYPH	denotes	-
T10258	34892-34893	CD	denotes	1
T10259	34899-34901	,	denotes	,
T10260	34901-34904	CC	denotes	but
T10261	34905-34908	RB	denotes	not
T10262	34909-34914	NN	denotes	mouse
T10263	34921-34929	NN	denotes	sterolin
T10264	34915-34920	NN	denotes	Abcg8
T10265	34920-34921	HYPH	denotes	/
T10266	34929-34930	HYPH	denotes	-
T10267	34930-34931	CD	denotes	2
T10268	34932-34933	-LRB-	denotes	(
T10269	34943-34945	NN	denotes	6c
T10270	34933-34939	NN	denotes	Figure
T10271	34940-34942	NN	denotes	6b
T10272	34942-34943	,	denotes	,
T10273	34945-34946	-RRB-	denotes	)
T10274	34946-34947	.	denotes	.
T10275	34947-35232	sentence	denotes	Co-transfection of COS-1 cells with both Abcg5 and Abcg8 cDNAs did not alter the pattern of immunofluorescence, although we are not able to confirm simultaneous expression of Abcg8 in cells expressing Abcg5/sterolin-1, as we have no suitable antibody for Abcg8/sterolin-2 (Figure 6d).
T10276	34948-34963	NN	denotes	Co-transfection
T10277	35019-35024	VB	denotes	alter
T10278	34964-34966	IN	denotes	of
T10279	34967-34970	NN	denotes	COS
T10280	34973-34978	NNS	denotes	cells
T10281	34970-34971	HYPH	denotes	-
T10282	34971-34972	CD	denotes	1
T10283	34979-34983	IN	denotes	with
T10284	34984-34988	CC	denotes	both
T10285	34989-34994	NN	denotes	Abcg5
T10286	35005-35010	NNS	denotes	cDNAs
T10287	34995-34998	CC	denotes	and
T10288	34999-35004	NN	denotes	Abcg8
T10289	35011-35014	VBD	denotes	did
T10290	35015-35018	RB	denotes	not
T10291	35025-35028	DT	denotes	the
T10292	35029-35036	NN	denotes	pattern
T10293	35037-35039	IN	denotes	of
T10294	35040-35058	NN	denotes	immunofluorescence
T10295	35058-35060	,	denotes	,
T10296	35060-35068	IN	denotes	although
T10297	35072-35075	VBP	denotes	are
T10298	35069-35071	PRP	denotes	we
T10299	35076-35079	RB	denotes	not
T10300	35080-35084	JJ	denotes	able
T10301	35085-35087	TO	denotes	to
T10302	35088-35095	VB	denotes	confirm
T10303	35096-35108	JJ	denotes	simultaneous
T10304	35109-35119	NN	denotes	expression
T10305	35120-35122	IN	denotes	of
T10306	35123-35128	NN	denotes	Abcg8
T10307	35129-35131	IN	denotes	in
T10308	35132-35137	NNS	denotes	cells
T10309	35138-35148	VBG	denotes	expressing
T10310	35149-35154	NN	denotes	Abcg5
T10311	35155-35163	NN	denotes	sterolin
T10312	35154-35155	HYPH	denotes	/
T10313	35163-35164	HYPH	denotes	-
T10314	35164-35165	CD	denotes	1
T10315	35165-35167	,	denotes	,
T10316	35167-35169	IN	denotes	as
T10317	35173-35177	VBP	denotes	have
T10318	35170-35172	PRP	denotes	we
T10319	35178-35180	DT	denotes	no
T10320	35190-35198	NN	denotes	antibody
T10321	35181-35189	JJ	denotes	suitable
T10322	35199-35202	IN	denotes	for
T10323	35203-35208	NN	denotes	Abcg8
T10324	35209-35217	NN	denotes	sterolin
T10325	35208-35209	HYPH	denotes	/
T10326	35217-35218	HYPH	denotes	-
T10327	35218-35219	CD	denotes	2
T10328	35220-35221	-LRB-	denotes	(
T10329	35228-35230	NN	denotes	6d
T10330	35221-35227	NN	denotes	Figure
T10331	35230-35231	-RRB-	denotes	)
T10332	35231-35232	.	denotes	.
T10333	35232-35372	sentence	denotes	Nevertheless, this experiment indicates that the SC antibody can recognize Abcg5/sterolin-1 but does not cross-react with Abcg8/sterolin-2.
T10334	35233-35245	RB	denotes	Nevertheless
T10335	35263-35272	VBZ	denotes	indicates
T10336	35245-35247	,	denotes	,
T10337	35247-35251	DT	denotes	this
T10338	35252-35262	NN	denotes	experiment
T10339	35273-35277	IN	denotes	that
T10340	35298-35307	VB	denotes	recognize
T10341	35278-35281	DT	denotes	the
T10342	35285-35293	NN	denotes	antibody
T10343	35282-35284	NN	denotes	SC
T10344	35294-35297	MD	denotes	can
T10345	35308-35313	NN	denotes	Abcg5
T10346	35314-35322	NN	denotes	sterolin
T10347	35313-35314	HYPH	denotes	/
T10348	35322-35323	HYPH	denotes	-
T10349	35323-35324	CD	denotes	1
T10350	35325-35328	CC	denotes	but
T10351	35329-35333	VBZ	denotes	does
T10352	35338-35349	VB	denotes	cross-react
T10353	35334-35337	RB	denotes	not
T10354	35350-35354	IN	denotes	with
T10355	35355-35360	NN	denotes	Abcg8
T10356	35361-35369	NN	denotes	sterolin
T10357	35360-35361	HYPH	denotes	/
T10358	35369-35370	HYPH	denotes	-
T10359	35370-35371	CD	denotes	2
T10360	35371-35372	.	denotes	.
T10361	35372-35507	sentence	denotes	Serial sections of intestinal tissue were incubated with anti-Abcg5/sterolin-1 to determine the cellular location of Abcg5/sterolin-1.
T10362	35373-35379	JJ	denotes	Serial
T10363	35380-35388	NNS	denotes	sections
T10364	35415-35424	VBN	denotes	incubated
T10365	35389-35391	IN	denotes	of
T10366	35392-35402	JJ	denotes	intestinal
T10367	35403-35409	NN	denotes	tissue
T10368	35410-35414	VBD	denotes	were
T10369	35425-35429	IN	denotes	with
T10370	35430-35440	NN	denotes	anti-Abcg5
T10371	35441-35449	NN	denotes	sterolin
T10372	35440-35441	HYPH	denotes	/
T10373	35449-35450	HYPH	denotes	-
T10374	35450-35451	CD	denotes	1
T10375	35452-35454	TO	denotes	to
T10376	35455-35464	VB	denotes	determine
T10377	35465-35468	DT	denotes	the
T10378	35478-35486	NN	denotes	location
T10379	35469-35477	JJ	denotes	cellular
T10380	35487-35489	IN	denotes	of
T10381	35490-35495	NN	denotes	Abcg5
T10382	35496-35504	NN	denotes	sterolin
T10383	35495-35496	HYPH	denotes	/
T10384	35504-35505	HYPH	denotes	-
T10385	35505-35506	CD	denotes	1
T10386	35506-35507	.	denotes	.
T10387	35507-35661	sentence	denotes	The pattern of staining of Abcg5/sterolin-1 in the intestinal sections is clearly apical and localized to the villi of the enterocytes (Figure 6g,6h,6i).
T10388	35508-35511	DT	denotes	The
T10389	35512-35519	NN	denotes	pattern
T10390	35579-35581	VBZ	denotes	is
T10391	35520-35522	IN	denotes	of
T10392	35523-35531	NN	denotes	staining
T10393	35532-35534	IN	denotes	of
T10394	35535-35540	NN	denotes	Abcg5
T10395	35541-35549	NN	denotes	sterolin
T10396	35540-35541	HYPH	denotes	/
T10397	35549-35550	HYPH	denotes	-
T10398	35550-35551	CD	denotes	1
T10399	35552-35554	IN	denotes	in
T10400	35555-35558	DT	denotes	the
T10401	35570-35578	NNS	denotes	sections
T10402	35559-35569	JJ	denotes	intestinal
T10403	35582-35589	RB	denotes	clearly
T10404	35590-35596	JJ	denotes	apical
T10405	35597-35600	CC	denotes	and
T10406	35601-35610	JJ	denotes	localized
T10407	35611-35613	IN	denotes	to
T10408	35614-35617	DT	denotes	the
T10409	35618-35623	NNS	denotes	villi
T10410	35624-35626	IN	denotes	of
T10411	35627-35630	DT	denotes	the
T10412	35631-35642	NNS	denotes	enterocytes
T10413	35643-35644	-LRB-	denotes	(
T10414	35657-35659	NN	denotes	6i
T10415	35644-35650	NN	denotes	Figure
T10416	35651-35653	NN	denotes	6g
T10417	35653-35654	,	denotes	,
T10418	35654-35656	NN	denotes	6h
T10419	35656-35657	,	denotes	,
T10420	35659-35660	-RRB-	denotes	)
T10421	35660-35661	.	denotes	.
T10422	35661-35736	sentence	denotes	Loss of Abcg8/sterolin-2 does not seem to affect this pattern of staining.
T10423	35662-35666	NN	denotes	Loss
T10424	35696-35700	VB	denotes	seem
T10425	35667-35669	IN	denotes	of
T10426	35670-35675	NN	denotes	Abcg8
T10427	35676-35684	NN	denotes	sterolin
T10428	35675-35676	HYPH	denotes	/
T10429	35684-35685	HYPH	denotes	-
T10430	35685-35686	CD	denotes	2
T10431	35687-35691	VBZ	denotes	does
T10432	35692-35695	RB	denotes	not
T10433	35701-35703	TO	denotes	to
T10434	35704-35710	VB	denotes	affect
T10435	35711-35715	DT	denotes	this
T10436	35716-35723	NN	denotes	pattern
T10437	35724-35726	IN	denotes	of
T10438	35727-35735	NN	denotes	staining
T10439	35735-35736	.	denotes	.
T10440	35736-35917	sentence	denotes	To further confirm that the antibody used recognizes Abcg5/sterolin-1; this pattern is blocked if the antibody is pre-incubated with the peptide to which it was raised (Figure 6f).
T10441	35737-35739	TO	denotes	To
T10442	35748-35755	VB	denotes	confirm
T10443	35740-35747	RB	denotes	further
T10444	35824-35831	VBN	denotes	blocked
T10445	35756-35760	IN	denotes	that
T10446	35779-35789	VBZ	denotes	recognizes
T10447	35761-35764	DT	denotes	the
T10448	35765-35773	NN	denotes	antibody
T10449	35774-35778	VBN	denotes	used
T10450	35790-35795	NN	denotes	Abcg5
T10451	35796-35804	NN	denotes	sterolin
T10452	35795-35796	HYPH	denotes	/
T10453	35804-35805	HYPH	denotes	-
T10454	35805-35806	CD	denotes	1
T10455	35806-35807	:	denotes	;
T10456	35808-35812	DT	denotes	this
T10457	35813-35820	NN	denotes	pattern
T10458	35821-35823	VBZ	denotes	is
T10459	35832-35834	IN	denotes	if
T10460	35851-35864	VBN	denotes	pre-incubated
T10461	35835-35838	DT	denotes	the
T10462	35839-35847	NN	denotes	antibody
T10463	35848-35850	VBZ	denotes	is
T10464	35865-35869	IN	denotes	with
T10465	35870-35873	DT	denotes	the
T10466	35874-35881	NN	denotes	peptide
T10467	35882-35884	IN	denotes	to
T10468	35898-35904	VBN	denotes	raised
T10469	35885-35890	WDT	denotes	which
T10470	35891-35893	PRP	denotes	it
T10471	35894-35897	VBD	denotes	was
T10472	35905-35906	-LRB-	denotes	(
T10473	35913-35915	NN	denotes	6f
T10474	35906-35912	NN	denotes	Figure
T10475	35915-35916	-RRB-	denotes	)
T10476	35916-35917	.	denotes	.
T10477	35917-36135	sentence	denotes	To further confirm that Abcg5/sterolin-1 expression is preserved and may be apical, immunohistochemistry was performed in Amsterdam using the AMC antibody to stain liver sections from wild-type and knockout mice [30].
T10478	35918-35920	TO	denotes	To
T10479	35929-35936	VB	denotes	confirm
T10480	35921-35928	RB	denotes	further
T10481	36027-36036	VBN	denotes	performed
T10482	35937-35941	IN	denotes	that
T10483	35973-35982	VBN	denotes	preserved
T10484	35942-35947	NN	denotes	Abcg5
T10485	35948-35956	NN	denotes	sterolin
T10486	35947-35948	HYPH	denotes	/
T10487	35959-35969	NN	denotes	expression
T10488	35956-35957	HYPH	denotes	-
T10489	35957-35958	CD	denotes	1
T10490	35970-35972	VBZ	denotes	is
T10491	35983-35986	CC	denotes	and
T10492	35987-35990	MD	denotes	may
T10493	35991-35993	VB	denotes	be
T10494	35994-36000	JJ	denotes	apical
T10495	36000-36002	,	denotes	,
T10496	36002-36022	NN	denotes	immunohistochemistry
T10497	36023-36026	VBD	denotes	was
T10498	36037-36039	IN	denotes	in
T10499	36040-36049	NN	denotes	Amsterdam
T10500	36050-36055	VBG	denotes	using
T10501	36056-36059	DT	denotes	the
T10502	36064-36072	NN	denotes	antibody
T10503	36060-36063	NN	denotes	AMC
T10504	36073-36075	TO	denotes	to
T10505	36076-36081	VB	denotes	stain
T10506	36082-36087	NN	denotes	liver
T10507	36088-36096	NNS	denotes	sections
T10508	36097-36101	IN	denotes	from
T10509	36102-36106	JJ	denotes	wild
T10510	36107-36111	NN	denotes	type
T10511	36106-36107	HYPH	denotes	-
T10512	36125-36129	NNS	denotes	mice
T10513	36112-36115	CC	denotes	and
T10514	36116-36124	NN	denotes	knockout
T10515	36130-36131	-LRB-	denotes	[
T10516	36131-36133	CD	denotes	30
T10517	36133-36134	-RRB-	denotes	]
T10518	36134-36135	.	denotes	.
T10519	36135-36305	sentence	denotes	As a control, the expression of Abcb11, an ABC transporter known to be responsible for the export of bile salts, was compared to that of Abcg5/sterolin-1 (Figure 6j,6k).
T10520	36136-36138	IN	denotes	As
T10521	36253-36261	VBN	denotes	compared
T10522	36139-36140	DT	denotes	a
T10523	36141-36148	NN	denotes	control
T10524	36148-36150	,	denotes	,
T10525	36150-36153	DT	denotes	the
T10526	36154-36164	NN	denotes	expression
T10527	36165-36167	IN	denotes	of
T10528	36168-36174	NN	denotes	Abcb11
T10529	36174-36176	,	denotes	,
T10530	36176-36178	DT	denotes	an
T10531	36183-36194	NN	denotes	transporter
T10532	36179-36182	NN	denotes	ABC
T10533	36195-36200	VBN	denotes	known
T10534	36201-36203	TO	denotes	to
T10535	36204-36206	VB	denotes	be
T10536	36207-36218	JJ	denotes	responsible
T10537	36219-36222	IN	denotes	for
T10538	36223-36226	DT	denotes	the
T10539	36227-36233	NN	denotes	export
T10540	36234-36236	IN	denotes	of
T10541	36237-36241	NN	denotes	bile
T10542	36242-36247	NNS	denotes	salts
T10543	36247-36249	,	denotes	,
T10544	36249-36252	VBD	denotes	was
T10545	36262-36264	IN	denotes	to
T10546	36265-36269	DT	denotes	that
T10547	36270-36272	IN	denotes	of
T10548	36273-36278	NN	denotes	Abcg5
T10549	36279-36287	NN	denotes	sterolin
T10550	36278-36279	HYPH	denotes	/
T10551	36287-36288	HYPH	denotes	-
T10552	36288-36289	CD	denotes	1
T10553	36290-36291	-LRB-	denotes	(
T10554	36301-36303	NN	denotes	6k
T10555	36291-36297	NN	denotes	Figure
T10556	36298-36300	NN	denotes	6j
T10557	36300-36301	,	denotes	,
T10558	36303-36304	-RRB-	denotes	)
T10559	36304-36305	.	denotes	.
T10560	36305-36564	sentence	denotes	Again, both proteins are apically localized in liver sections from wild-type mice and this pattern is essentially unperturbed in liver from knockout mice, although the expression level of Abcg5/sterolin-1 seems to be less robust qualitatively (Figure 6l,6m).
T10561	36306-36311	RB	denotes	Again
T10562	36340-36349	VBN	denotes	localized
T10563	36311-36313	,	denotes	,
T10564	36313-36317	DT	denotes	both
T10565	36318-36326	NN	denotes	proteins
T10566	36327-36330	VBP	denotes	are
T10567	36331-36339	RB	denotes	apically
T10568	36350-36352	IN	denotes	in
T10569	36353-36358	NN	denotes	liver
T10570	36359-36367	NNS	denotes	sections
T10571	36368-36372	IN	denotes	from
T10572	36373-36377	JJ	denotes	wild
T10573	36378-36382	NN	denotes	type
T10574	36377-36378	HYPH	denotes	-
T10575	36383-36387	NNS	denotes	mice
T10576	36388-36391	CC	denotes	and
T10577	36392-36396	DT	denotes	this
T10578	36397-36404	NN	denotes	pattern
T10579	36405-36407	VBZ	denotes	is
T10580	36408-36419	RB	denotes	essentially
T10581	36420-36431	JJ	denotes	unperturbed
T10582	36432-36434	IN	denotes	in
T10583	36435-36440	NN	denotes	liver
T10584	36441-36445	IN	denotes	from
T10585	36446-36454	NN	denotes	knockout
T10586	36455-36459	NNS	denotes	mice
T10587	36459-36461	,	denotes	,
T10588	36461-36469	IN	denotes	although
T10589	36511-36516	VBZ	denotes	seems
T10590	36470-36473	DT	denotes	the
T10591	36485-36490	NN	denotes	level
T10592	36474-36484	NN	denotes	expression
T10593	36491-36493	IN	denotes	of
T10594	36494-36499	NN	denotes	Abcg5
T10595	36500-36508	NN	denotes	sterolin
T10596	36499-36500	HYPH	denotes	/
T10597	36508-36509	HYPH	denotes	-
T10598	36509-36510	CD	denotes	1
T10599	36517-36519	TO	denotes	to
T10600	36520-36522	VB	denotes	be
T10601	36523-36527	RBR	denotes	less
T10602	36528-36534	JJ	denotes	robust
T10603	36535-36548	RB	denotes	qualitatively
T10604	36549-36550	-LRB-	denotes	(
T10605	36560-36562	NN	denotes	6m
T10606	36550-36556	NN	denotes	Figure
T10607	36557-36559	NN	denotes	6l
T10608	36559-36560	,	denotes	,
T10609	36562-36563	-RRB-	denotes	)
T10610	36563-36564	.	denotes	.
T10611	36564-36730	sentence	denotes	To further confirm the apical expression of Abcg5/sterolin-1 in the knockout mice, the UTSW antibody was used to stain liver sections of wild-type and knockout mice.
T10612	36565-36567	TO	denotes	To
T10613	36576-36583	VB	denotes	confirm
T10614	36568-36575	RB	denotes	further
T10615	36670-36674	VBN	denotes	used
T10616	36584-36587	DT	denotes	the
T10617	36595-36605	NN	denotes	expression
T10618	36588-36594	JJ	denotes	apical
T10619	36606-36608	IN	denotes	of
T10620	36609-36614	NN	denotes	Abcg5
T10621	36615-36623	NN	denotes	sterolin
T10622	36614-36615	HYPH	denotes	/
T10623	36623-36624	HYPH	denotes	-
T10624	36624-36625	CD	denotes	1
T10625	36626-36628	IN	denotes	in
T10626	36629-36632	DT	denotes	the
T10627	36642-36646	NNS	denotes	mice
T10628	36633-36641	NN	denotes	knockout
T10629	36646-36648	,	denotes	,
T10630	36648-36651	DT	denotes	the
T10631	36657-36665	NN	denotes	antibody
T10632	36652-36656	NN	denotes	UTSW
T10633	36666-36669	VBD	denotes	was
T10634	36675-36677	TO	denotes	to
T10635	36678-36683	VB	denotes	stain
T10636	36684-36689	NN	denotes	liver
T10637	36690-36698	NNS	denotes	sections
T10638	36699-36701	IN	denotes	of
T10639	36702-36706	JJ	denotes	wild
T10640	36707-36711	NN	denotes	type
T10641	36706-36707	HYPH	denotes	-
T10642	36725-36729	NNS	denotes	mice
T10643	36712-36715	CC	denotes	and
T10644	36716-36724	NN	denotes	knockout
T10645	36729-36730	.	denotes	.
T10646	36730-36838	sentence	denotes	Again, there is apical expression of Abcg5/sterolin-1 in both wild-type and knockout livers (Figure 7a,7b).
T10647	36731-36736	RB	denotes	Again
T10648	36744-36746	VBZ	denotes	is
T10649	36736-36738	,	denotes	,
T10650	36738-36743	EX	denotes	there
T10651	36747-36753	JJ	denotes	apical
T10652	36754-36764	NN	denotes	expression
T10653	36765-36767	IN	denotes	of
T10654	36768-36773	NN	denotes	Abcg5
T10655	36774-36782	NN	denotes	sterolin
T10656	36773-36774	HYPH	denotes	/
T10657	36782-36783	HYPH	denotes	-
T10658	36783-36784	CD	denotes	1
T10659	36785-36787	IN	denotes	in
T10660	36788-36792	CC	denotes	both
T10661	36798-36802	NN	denotes	type
T10662	36793-36797	JJ	denotes	wild
T10663	36797-36798	HYPH	denotes	-
T10664	36816-36822	NNS	denotes	livers
T10665	36803-36806	CC	denotes	and
T10666	36807-36815	NN	denotes	knockout
T10667	36823-36824	-LRB-	denotes	(
T10668	36834-36836	NN	denotes	7b
T10669	36824-36830	NN	denotes	Figure
T10670	36831-36833	NN	denotes	7a
T10671	36833-36834	,	denotes	,
T10672	36836-36837	-RRB-	denotes	)
T10673	36837-36838	.	denotes	.
T10961	36840-36847	JJ	denotes	Biliary
T10962	36848-36857	NN	denotes	secretion
T10963	36858-36860	IN	denotes	of
T10964	36861-36865	NN	denotes	bile
T10965	36866-36871	NNS	denotes	salts
T10966	36871-36873	,	denotes	,
T10967	36873-36879	NN	denotes	sterol
T10968	36880-36883	CC	denotes	and
T10969	36884-36897	NNS	denotes	phospholipids
T10970	36898-36900	IN	denotes	in
T10971	36901-36906	NN	denotes	Abcg8
T10972	36907-36915	NN	denotes	sterolin
T10973	36906-36907	HYPH	denotes	/
T10974	36918-36927	JJ	denotes	deficient
T10975	36915-36916	HYPH	denotes	-
T10976	36916-36917	CD	denotes	2
T10977	36928-36932	NNS	denotes	mice
T10978	36932-37044	sentence	denotes	Sitosterolemic individuals have an impaired ability to secrete cholesterol and plant sterols into bile [40,41].
T10979	36933-36947	JJ	denotes	Sitosterolemic
T10980	36948-36959	NNS	denotes	individuals
T10981	36960-36964	VBP	denotes	have
T10982	36965-36967	DT	denotes	an
T10983	36977-36984	NN	denotes	ability
T10984	36968-36976	JJ	denotes	impaired
T10985	36985-36987	TO	denotes	to
T10986	36988-36995	VB	denotes	secrete
T10987	36996-37007	NN	denotes	cholesterol
T10988	37008-37011	CC	denotes	and
T10989	37012-37017	NN	denotes	plant
T10990	37018-37025	NNS	denotes	sterols
T10991	37026-37030	IN	denotes	into
T10992	37031-37035	NN	denotes	bile
T10993	37036-37037	-LRB-	denotes	[
T10994	37040-37042	CD	denotes	41
T10995	37037-37039	CD	denotes	40
T10996	37039-37040	,	denotes	,
T10997	37042-37043	-RRB-	denotes	]
T10998	37043-37044	.	denotes	.
T10999	37044-37122	sentence	denotes	We analyzed the knockout mice for any alterations in biliary sterol handling.
T11000	37045-37047	PRP	denotes	We
T11001	37048-37056	VBD	denotes	analyzed
T11002	37057-37060	DT	denotes	the
T11003	37070-37074	NNS	denotes	mice
T11004	37061-37069	NN	denotes	knockout
T11005	37075-37078	IN	denotes	for
T11006	37079-37082	DT	denotes	any
T11007	37083-37094	NNS	denotes	alterations
T11008	37095-37097	IN	denotes	in
T11009	37098-37105	JJ	denotes	biliary
T11010	37106-37112	NN	denotes	sterol
T11011	37113-37121	NN	denotes	handling
T11012	37121-37122	.	denotes	.
T11013	37122-37418	sentence	denotes	Analyses of the initial basal bile secretion showed that the sterol and phospholipid contents in Abcg8-/- mice were reduced as compared to wild-type mice, (Figure 8a,8b,8c, bile salt secretion not statistically significant, for sterol secretion P = 0.01 and for phospholipid secretion P = 0.03).
T11014	37123-37131	NNS	denotes	Analyses
T11015	37168-37174	VBD	denotes	showed
T11016	37132-37134	IN	denotes	of
T11017	37135-37138	DT	denotes	the
T11018	37158-37167	NN	denotes	secretion
T11019	37139-37146	JJ	denotes	initial
T11020	37147-37152	JJ	denotes	basal
T11021	37153-37157	NN	denotes	bile
T11022	37175-37179	IN	denotes	that
T11023	37239-37246	VBN	denotes	reduced
T11024	37180-37183	DT	denotes	the
T11025	37208-37216	NNS	denotes	contents
T11026	37184-37190	NN	denotes	sterol
T11027	37191-37194	CC	denotes	and
T11028	37195-37207	NN	denotes	phospholipid
T11029	37217-37219	IN	denotes	in
T11030	37220-37225	NN	denotes	Abcg8
T11031	37229-37233	NNS	denotes	mice
T11032	37225-37226	SYM	denotes	-
T11033	37226-37227	HYPH	denotes	/
T11034	37227-37228	SYM	denotes	-
T11035	37234-37238	VBD	denotes	were
T11036	37247-37249	IN	denotes	as
T11037	37250-37258	VBN	denotes	compared
T11038	37259-37261	IN	denotes	to
T11039	37262-37266	JJ	denotes	wild
T11040	37267-37271	NN	denotes	type
T11041	37266-37267	HYPH	denotes	-
T11042	37272-37276	NNS	denotes	mice
T11043	37276-37278	,	denotes	,
T11044	37278-37279	-LRB-	denotes	(
T11045	37292-37294	NN	denotes	8c
T11046	37279-37285	NN	denotes	Figure
T11047	37286-37288	NN	denotes	8a
T11048	37288-37289	,	denotes	,
T11049	37289-37291	NN	denotes	8b
T11050	37291-37292	,	denotes	,
T11051	37294-37296	,	denotes	,
T11052	37296-37300	NN	denotes	bile
T11053	37301-37305	NN	denotes	salt
T11054	37306-37315	NN	denotes	secretion
T11055	37334-37345	JJ	denotes	significant
T11056	37316-37319	RB	denotes	not
T11057	37320-37333	RB	denotes	statistically
T11058	37372-37376	CD	denotes	0.01
T11059	37345-37347	,	denotes	,
T11060	37347-37350	IN	denotes	for
T11061	37351-37357	NN	denotes	sterol
T11062	37358-37367	NN	denotes	secretion
T11063	37368-37369	NN	denotes	P
T11064	37370-37371	SYM	denotes	=
T11065	37377-37380	CC	denotes	and
T11066	37381-37384	IN	denotes	for
T11067	37412-37416	CD	denotes	0.03
T11068	37385-37397	NN	denotes	phospholipid
T11069	37398-37407	NN	denotes	secretion
T11070	37408-37409	NN	denotes	P
T11071	37410-37411	SYM	denotes	=
T11072	37416-37417	-RRB-	denotes	)
T11073	37417-37418	.	denotes	.
T11074	37418-37627	sentence	denotes	To investigate whether the defect in biliary sterol secretion could be (partly) restored by forced biliary sterol secretion, we infused mice with stepwise increasing doses of tauroursodeoxycholic acid (TUDC).
T11075	37419-37421	TO	denotes	To
T11076	37422-37433	VB	denotes	investigate
T11077	37547-37554	VBD	denotes	infused
T11078	37434-37441	IN	denotes	whether
T11079	37499-37507	VBN	denotes	restored
T11080	37442-37445	DT	denotes	the
T11081	37446-37452	NN	denotes	defect
T11082	37453-37455	IN	denotes	in
T11083	37456-37463	JJ	denotes	biliary
T11084	37471-37480	NN	denotes	secretion
T11085	37464-37470	NN	denotes	sterol
T11086	37481-37486	MD	denotes	could
T11087	37487-37489	VB	denotes	be
T11088	37490-37491	-LRB-	denotes	(
T11089	37491-37497	RB	denotes	partly
T11090	37497-37498	-RRB-	denotes	)
T11091	37508-37510	IN	denotes	by
T11092	37511-37517	VBN	denotes	forced
T11093	37533-37542	NN	denotes	secretion
T11094	37518-37525	JJ	denotes	biliary
T11095	37526-37532	NN	denotes	sterol
T11096	37542-37544	,	denotes	,
T11097	37544-37546	PRP	denotes	we
T11098	37555-37559	NNS	denotes	mice
T11099	37560-37564	IN	denotes	with
T11100	37565-37573	JJ	denotes	stepwise
T11101	37574-37584	VBG	denotes	increasing
T11102	37585-37590	NNS	denotes	doses
T11103	37591-37593	IN	denotes	of
T11104	37594-37614	JJ	denotes	tauroursodeoxycholic
T11105	37615-37619	NN	denotes	acid
T11106	37620-37621	-LRB-	denotes	(
T11107	37621-37625	NN	denotes	TUDC
T11108	37625-37626	-RRB-	denotes	)
T11109	37626-37627	.	denotes	.
T11110	37627-37776	sentence	denotes	Mice were first depleted of their endogenous bile salt pools for 90 min and subsequently infused via the jugular vein with increasing doses of TUDC.
T11111	37628-37632	NNS	denotes	Mice
T11112	37644-37652	VBN	denotes	depleted
T11113	37633-37637	VBD	denotes	were
T11114	37638-37643	RB	denotes	first
T11115	37653-37655	IN	denotes	of
T11116	37656-37661	PRP$	denotes	their
T11117	37683-37688	NNS	denotes	pools
T11118	37662-37672	JJ	denotes	endogenous
T11119	37673-37677	NN	denotes	bile
T11120	37678-37682	NN	denotes	salt
T11121	37689-37692	IN	denotes	for
T11122	37693-37695	CD	denotes	90
T11123	37696-37699	NN	denotes	min
T11124	37700-37703	CC	denotes	and
T11125	37704-37716	RB	denotes	subsequently
T11126	37717-37724	VBN	denotes	infused
T11127	37725-37728	IN	denotes	via
T11128	37729-37732	DT	denotes	the
T11129	37741-37745	NN	denotes	vein
T11130	37733-37740	JJ	denotes	jugular
T11131	37746-37750	IN	denotes	with
T11132	37751-37761	VBG	denotes	increasing
T11133	37762-37767	NNS	denotes	doses
T11134	37768-37770	IN	denotes	of
T11135	37771-37775	NN	denotes	TUDC
T11136	37775-37776	.	denotes	.
T11137	37776-37936	sentence	denotes	As shown in Figure 8d, bile salt secretion was no different between the different genotypes during the depletion or the TUDC infusion phases of the experiment.
T11138	37777-37779	IN	denotes	As
T11139	37780-37785	VBN	denotes	shown
T11140	37820-37823	VBD	denotes	was
T11141	37786-37788	IN	denotes	in
T11142	37789-37795	NN	denotes	Figure
T11143	37796-37798	NN	denotes	8d
T11144	37798-37800	,	denotes	,
T11145	37800-37804	NN	denotes	bile
T11146	37805-37809	NN	denotes	salt
T11147	37810-37819	NN	denotes	secretion
T11148	37824-37826	RB	denotes	no
T11149	37827-37836	JJ	denotes	different
T11150	37837-37844	IN	denotes	between
T11151	37845-37848	DT	denotes	the
T11152	37859-37868	NNS	denotes	genotypes
T11153	37849-37858	JJ	denotes	different
T11154	37869-37875	IN	denotes	during
T11155	37876-37879	DT	denotes	the
T11156	37880-37889	NN	denotes	depletion
T11157	37890-37892	CC	denotes	or
T11158	37893-37896	DT	denotes	the
T11159	37902-37910	NN	denotes	infusion
T11160	37897-37901	NN	denotes	TUDC
T11161	37911-37917	NNS	denotes	phases
T11162	37918-37920	IN	denotes	of
T11163	37921-37924	DT	denotes	the
T11164	37925-37935	NN	denotes	experiment
T11165	37935-37936	.	denotes	.
T11166	37936-38020	sentence	denotes	However, a different pattern emerged for the secretion of phospholipid (Figure 8f).
T11167	37937-37944	RB	denotes	However
T11168	37966-37973	VBD	denotes	emerged
T11169	37944-37946	,	denotes	,
T11170	37946-37947	DT	denotes	a
T11171	37958-37965	NN	denotes	pattern
T11172	37948-37957	JJ	denotes	different
T11173	37974-37977	IN	denotes	for
T11174	37978-37981	DT	denotes	the
T11175	37982-37991	NN	denotes	secretion
T11176	37992-37994	IN	denotes	of
T11177	37995-38007	NN	denotes	phospholipid
T11178	38008-38009	-LRB-	denotes	(
T11179	38016-38018	NN	denotes	8f
T11180	38009-38015	NN	denotes	Figure
T11181	38018-38019	-RRB-	denotes	)
T11182	38019-38020	.	denotes	.
T11183	38020-38196	sentence	denotes	There was no difference between wild-type and heterozygous mice, but knockout mice showed a trend of lower phospholipid secretion during both the depletion and infusion phase.
T11184	38021-38026	EX	denotes	There
T11185	38027-38030	VBD	denotes	was
T11186	38031-38033	DT	denotes	no
T11187	38034-38044	NN	denotes	difference
T11188	38045-38052	IN	denotes	between
T11189	38053-38057	JJ	denotes	wild
T11190	38058-38062	NN	denotes	type
T11191	38057-38058	HYPH	denotes	-
T11192	38080-38084	NNS	denotes	mice
T11193	38063-38066	CC	denotes	and
T11194	38067-38079	JJ	denotes	heterozygous
T11195	38084-38086	,	denotes	,
T11196	38086-38089	CC	denotes	but
T11197	38090-38098	NN	denotes	knockout
T11198	38099-38103	NNS	denotes	mice
T11199	38104-38110	VBD	denotes	showed
T11200	38111-38112	DT	denotes	a
T11201	38113-38118	NN	denotes	trend
T11202	38119-38121	IN	denotes	of
T11203	38122-38127	JJR	denotes	lower
T11204	38141-38150	NN	denotes	secretion
T11205	38128-38140	NN	denotes	phospholipid
T11206	38151-38157	IN	denotes	during
T11207	38158-38162	CC	denotes	both
T11208	38190-38195	NN	denotes	phase
T11209	38163-38166	DT	denotes	the
T11210	38167-38176	NN	denotes	depletion
T11211	38177-38180	CC	denotes	and
T11212	38181-38189	NN	denotes	infusion
T11213	38195-38196	.	denotes	.
T11214	38196-38420	sentence	denotes	Even more dramatic was the effect on biliary sterol secretion although the sterol contents were determined enzymatically and no distinction is made as to whether this is sitosterol or cholesterol (Figure 8e, but see below).
T11215	38197-38201	RB	denotes	Even
T11216	38202-38206	RBR	denotes	more
T11217	38207-38215	JJ	denotes	dramatic
T11218	38216-38219	VBD	denotes	was
T11219	38220-38223	DT	denotes	the
T11220	38224-38230	NN	denotes	effect
T11221	38231-38233	IN	denotes	on
T11222	38234-38241	JJ	denotes	biliary
T11223	38242-38248	NN	denotes	sterol
T11224	38249-38258	NN	denotes	secretion
T11225	38259-38267	IN	denotes	although
T11226	38293-38303	VBN	denotes	determined
T11227	38268-38271	DT	denotes	the
T11228	38279-38287	NNS	denotes	contents
T11229	38272-38278	NN	denotes	sterol
T11230	38288-38292	VBD	denotes	were
T11231	38304-38317	RB	denotes	enzymatically
T11232	38318-38321	CC	denotes	and
T11233	38322-38324	DT	denotes	no
T11234	38325-38336	NN	denotes	distinction
T11235	38340-38344	VBN	denotes	made
T11236	38337-38339	VBZ	denotes	is
T11237	38345-38347	IN	denotes	as
T11238	38348-38350	IN	denotes	to
T11239	38351-38358	IN	denotes	whether
T11240	38364-38366	VBZ	denotes	is
T11241	38359-38363	DT	denotes	this
T11242	38367-38377	NN	denotes	sitosterol
T11243	38378-38380	CC	denotes	or
T11244	38381-38392	NN	denotes	cholesterol
T11245	38393-38394	-LRB-	denotes	(
T11246	38401-38403	NN	denotes	8e
T11247	38394-38400	NN	denotes	Figure
T11248	38403-38405	,	denotes	,
T11249	38405-38408	CC	denotes	but
T11250	38409-38412	VB	denotes	see
T11251	38413-38418	RB	denotes	below
T11252	38418-38419	-RRB-	denotes	)
T11253	38419-38420	.	denotes	.
T11254	38420-38548	sentence	denotes	In the knockout mice, almost no stimulated sterol secretion was noted, with an intermediate phenotype in the heterozygous mice.
T11255	38421-38423	IN	denotes	In
T11256	38485-38490	VBN	denotes	noted
T11257	38424-38427	DT	denotes	the
T11258	38437-38441	NNS	denotes	mice
T11259	38428-38436	NN	denotes	knockout
T11260	38441-38443	,	denotes	,
T11261	38443-38449	RB	denotes	almost
T11262	38471-38480	NN	denotes	secretion
T11263	38450-38452	DT	denotes	no
T11264	38453-38463	VBN	denotes	stimulated
T11265	38464-38470	NN	denotes	sterol
T11266	38481-38484	VBD	denotes	was
T11267	38490-38492	,	denotes	,
T11268	38492-38496	IN	denotes	with
T11269	38497-38499	DT	denotes	an
T11270	38513-38522	NN	denotes	phenotype
T11271	38500-38512	JJ	denotes	intermediate
T11272	38523-38525	IN	denotes	in
T11273	38526-38529	DT	denotes	the
T11274	38543-38547	NNS	denotes	mice
T11275	38530-38542	JJ	denotes	heterozygous
T11276	38547-38548	.	denotes	.
T11277	38548-38750	sentence	denotes	In the heterozygous mice, sterol secretion increased to reach a maximal level of about 50% of the wild type suggesting that Abcg8/sterolin-2 is a rate-limiting step for biliary 'cholesterol' secretion.
T11278	38549-38551	IN	denotes	In
T11279	38592-38601	VBD	denotes	increased
T11280	38552-38555	DT	denotes	the
T11281	38569-38573	NNS	denotes	mice
T11282	38556-38568	JJ	denotes	heterozygous
T11283	38573-38575	,	denotes	,
T11284	38575-38581	NN	denotes	sterol
T11285	38582-38591	NN	denotes	secretion
T11286	38602-38604	TO	denotes	to
T11287	38605-38610	VB	denotes	reach
T11288	38611-38612	DT	denotes	a
T11289	38621-38626	NN	denotes	level
T11290	38613-38620	JJ	denotes	maximal
T11291	38627-38629	IN	denotes	of
T11292	38630-38635	IN	denotes	about
T11293	38636-38638	CD	denotes	50
T11294	38638-38639	NN	denotes	%
T11295	38640-38642	IN	denotes	of
T11296	38643-38646	DT	denotes	the
T11297	38652-38656	NN	denotes	type
T11298	38647-38651	JJ	denotes	wild
T11299	38657-38667	VBG	denotes	suggesting
T11300	38668-38672	IN	denotes	that
T11301	38690-38692	VBZ	denotes	is
T11302	38673-38678	NN	denotes	Abcg8
T11303	38679-38687	NN	denotes	sterolin
T11304	38678-38679	HYPH	denotes	/
T11305	38687-38688	HYPH	denotes	-
T11306	38688-38689	CD	denotes	2
T11307	38693-38694	DT	denotes	a
T11308	38709-38713	NN	denotes	step
T11309	38695-38699	NN	denotes	rate
T11310	38700-38708	VBG	denotes	limiting
T11311	38699-38700	HYPH	denotes	-
T11312	38714-38717	IN	denotes	for
T11313	38718-38725	JJ	denotes	biliary
T11314	38740-38749	NN	denotes	secretion
T11315	38726-38727	``	denotes	'
T11316	38727-38738	NN	denotes	cholesterol
T11317	38738-38739	''	denotes	'
T11318	38749-38750	.	denotes	.
T11319	38750-38892	sentence	denotes	In the knockout mice, sterol secretion remained at a constant level during the depletion phase and increased minimally upon infusion of TUDC.
T11320	38751-38753	IN	denotes	In
T11321	38790-38798	VBD	denotes	remained
T11322	38754-38757	DT	denotes	the
T11323	38767-38771	NNS	denotes	mice
T11324	38758-38766	NN	denotes	knockout
T11325	38771-38773	,	denotes	,
T11326	38773-38779	NN	denotes	sterol
T11327	38780-38789	NN	denotes	secretion
T11328	38799-38801	IN	denotes	at
T11329	38802-38803	DT	denotes	a
T11330	38813-38818	NN	denotes	level
T11331	38804-38812	JJ	denotes	constant
T11332	38819-38825	IN	denotes	during
T11333	38826-38829	DT	denotes	the
T11334	38840-38845	NN	denotes	phase
T11335	38830-38839	NN	denotes	depletion
T11336	38846-38849	CC	denotes	and
T11337	38850-38859	VBN	denotes	increased
T11338	38860-38869	RB	denotes	minimally
T11339	38870-38874	IN	denotes	upon
T11340	38875-38883	NN	denotes	infusion
T11341	38884-38886	IN	denotes	of
T11342	38887-38891	NN	denotes	TUDC
T11343	38891-38892	.	denotes	.
T11344	38892-39016	sentence	denotes	Since the Abcg8-/- mice still secreted some sterols we were interested in which biliary sterol species were being secreted.
T11345	38893-38898	IN	denotes	Since
T11346	38923-38931	VBD	denotes	secreted
T11347	38899-38902	DT	denotes	the
T11348	38912-38916	NNS	denotes	mice
T11349	38903-38908	NN	denotes	Abcg8
T11350	38908-38909	SYM	denotes	-
T11351	38909-38910	HYPH	denotes	/
T11352	38910-38911	SYM	denotes	-
T11353	38917-38922	RB	denotes	still
T11354	38948-38952	VBD	denotes	were
T11355	38932-38936	DT	denotes	some
T11356	38937-38944	NNS	denotes	sterols
T11357	38945-38947	PRP	denotes	we
T11358	38953-38963	JJ	denotes	interested
T11359	38964-38966	IN	denotes	in
T11360	38967-38972	WDT	denotes	which
T11361	38988-38995	NNS	denotes	species
T11362	38973-38980	JJ	denotes	biliary
T11363	38981-38987	NN	denotes	sterol
T11364	39007-39015	VBN	denotes	secreted
T11365	38996-39000	VBD	denotes	were
T11366	39001-39006	VBG	denotes	being
T11367	39015-39016	.	denotes	.
T11368	39016-39110	sentence	denotes	Therefore bile was collected from mice during a constant infusion of TUDC and analyzed by GC.
T11369	39017-39026	RB	denotes	Therefore
T11370	39036-39045	VBN	denotes	collected
T11371	39027-39031	NN	denotes	bile
T11372	39032-39035	VBD	denotes	was
T11373	39046-39050	IN	denotes	from
T11374	39051-39055	NNS	denotes	mice
T11375	39056-39062	IN	denotes	during
T11376	39063-39064	DT	denotes	a
T11377	39074-39082	NN	denotes	infusion
T11378	39065-39073	JJ	denotes	constant
T11379	39083-39085	IN	denotes	of
T11380	39086-39090	NN	denotes	TUDC
T11381	39091-39094	CC	denotes	and
T11382	39095-39103	VBN	denotes	analyzed
T11383	39104-39106	IN	denotes	by
T11384	39107-39109	NN	denotes	GC
T11385	39109-39110	.	denotes	.
T11386	39110-39266	sentence	denotes	Mice were first depleted of their endogenous bile acid pools for 30 minutes then infused with a continuous dose of TUDC (1,200 nmol/min/100 g body weight).
T11387	39111-39115	NNS	denotes	Mice
T11388	39127-39135	VBN	denotes	depleted
T11389	39116-39120	VBD	denotes	were
T11390	39121-39126	RB	denotes	first
T11391	39136-39138	IN	denotes	of
T11392	39139-39144	PRP$	denotes	their
T11393	39166-39171	NNS	denotes	pools
T11394	39145-39155	JJ	denotes	endogenous
T11395	39156-39160	NN	denotes	bile
T11396	39161-39165	NN	denotes	acid
T11397	39172-39175	IN	denotes	for
T11398	39176-39178	CD	denotes	30
T11399	39179-39186	NNS	denotes	minutes
T11400	39187-39191	RB	denotes	then
T11401	39192-39199	VBN	denotes	infused
T11402	39200-39204	IN	denotes	with
T11403	39205-39206	DT	denotes	a
T11404	39218-39222	NN	denotes	dose
T11405	39207-39217	JJ	denotes	continuous
T11406	39223-39225	IN	denotes	of
T11407	39226-39230	NN	denotes	TUDC
T11408	39231-39232	-LRB-	denotes	(
T11409	39238-39242	NN	denotes	nmol
T11410	39232-39237	CD	denotes	1,200
T11411	39242-39243	SYM	denotes	/
T11412	39243-39246	NN	denotes	min
T11413	39246-39247	SYM	denotes	/
T11414	39258-39264	NN	denotes	weight
T11415	39247-39250	NN	denotes	100
T11416	39251-39252	NN	denotes	g
T11417	39253-39257	NN	denotes	body
T11418	39264-39265	-RRB-	denotes	)
T11419	39265-39266	.	denotes	.
T11420	39266-39460	sentence	denotes	The knockout mice showed a significantly diminished ability to secrete cholesterol, yet still maintained the ability to secrete sitosterol and campesterol compared to wild-type mice (Figure 9).
T11421	39267-39270	DT	denotes	The
T11422	39280-39284	NNS	denotes	mice
T11423	39271-39279	NN	denotes	knockout
T11424	39285-39291	VBD	denotes	showed
T11425	39292-39293	DT	denotes	a
T11426	39319-39326	NN	denotes	ability
T11427	39294-39307	RB	denotes	significantly
T11428	39308-39318	VBN	denotes	diminished
T11429	39327-39329	TO	denotes	to
T11430	39330-39337	VB	denotes	secrete
T11431	39338-39349	NN	denotes	cholesterol
T11432	39349-39351	,	denotes	,
T11433	39351-39354	CC	denotes	yet
T11434	39355-39360	RB	denotes	still
T11435	39361-39371	VBD	denotes	maintained
T11436	39372-39375	DT	denotes	the
T11437	39376-39383	NN	denotes	ability
T11438	39384-39386	TO	denotes	to
T11439	39387-39394	VB	denotes	secrete
T11440	39395-39405	NN	denotes	sitosterol
T11441	39406-39409	CC	denotes	and
T11442	39410-39421	NN	denotes	campesterol
T11443	39422-39430	VBN	denotes	compared
T11444	39431-39433	IN	denotes	to
T11445	39434-39438	JJ	denotes	wild
T11446	39439-39443	NN	denotes	type
T11447	39438-39439	HYPH	denotes	-
T11448	39444-39448	NNS	denotes	mice
T11449	39449-39450	-LRB-	denotes	(
T11450	39450-39456	NN	denotes	Figure
T11451	39457-39458	CD	denotes	9
T11452	39458-39459	-RRB-	denotes	)
T11453	39459-39460	.	denotes	.
T11454	39460-39645	sentence	denotes	Surprisingly, the Abcg8+/- mice tended to show an increased ability to secrete all sterols above the levels seen in the wild-type mice, although this was not statistically significant.
T11455	39461-39473	RB	denotes	Surprisingly
T11456	39493-39499	VBD	denotes	tended
T11457	39473-39475	,	denotes	,
T11458	39475-39478	DT	denotes	the
T11459	39488-39492	NNS	denotes	mice
T11460	39479-39484	NN	denotes	Abcg8
T11461	39484-39485	SYM	denotes	+
T11462	39485-39486	HYPH	denotes	/
T11463	39486-39487	SYM	denotes	-
T11464	39500-39502	TO	denotes	to
T11465	39503-39507	VB	denotes	show
T11466	39508-39510	DT	denotes	an
T11467	39521-39528	NN	denotes	ability
T11468	39511-39520	VBN	denotes	increased
T11469	39529-39531	TO	denotes	to
T11470	39532-39539	VB	denotes	secrete
T11471	39540-39543	DT	denotes	all
T11472	39544-39551	NNS	denotes	sterols
T11473	39552-39557	IN	denotes	above
T11474	39558-39561	DT	denotes	the
T11475	39562-39568	NNS	denotes	levels
T11476	39569-39573	VBN	denotes	seen
T11477	39574-39576	IN	denotes	in
T11478	39577-39580	DT	denotes	the
T11479	39591-39595	NNS	denotes	mice
T11480	39581-39585	JJ	denotes	wild
T11481	39586-39590	NN	denotes	type
T11482	39585-39586	HYPH	denotes	-
T11483	39595-39597	,	denotes	,
T11484	39597-39605	IN	denotes	although
T11485	39611-39614	VBD	denotes	was
T11486	39606-39610	DT	denotes	this
T11487	39615-39618	RB	denotes	not
T11488	39619-39632	RB	denotes	statistically
T11489	39633-39644	JJ	denotes	significant
T11490	39644-39645	.	denotes	.
T12567	39658-39661	DT	denotes	The
T12568	39662-39671	NN	denotes	mechanism
T12569	39865-39875	VBN	denotes	elucidated
T12570	39672-39674	IN	denotes	by
T12571	39717-39725	VBN	denotes	absorbed
T12572	39675-39680	WDT	denotes	which
T12573	39681-39688	JJ	denotes	dietary
T12574	39689-39700	NN	denotes	cholesterol
T12575	39701-39703	VBZ	denotes	is
T12576	39704-39716	RB	denotes	specifically
T12577	39726-39729	CC	denotes	and
T12578	39730-39737	JJ	denotes	dietary
T12579	39754-39761	NNS	denotes	sterols
T12580	39738-39753	NN	denotes	non-cholesterol
T12581	39772-39780	VBD	denotes	excluded
T12582	39762-39771	RB	denotes	primarily
T12583	39781-39783	CC	denotes	or
T12584	39784-39787	DT	denotes	the
T12585	39788-39797	NN	denotes	mechanism
T12586	39797-39798	-LRB-	denotes	(
T12587	39798-39799	AFX	denotes	s
T12588	39799-39800	-RRB-	denotes	)
T12589	39801-39803	IN	denotes	by
T12590	39836-39843	VB	denotes	secrete
T12591	39804-39809	WDT	denotes	which
T12592	39810-39813	DT	denotes	the
T12593	39814-39819	NN	denotes	liver
T12594	39820-39823	MD	denotes	can
T12595	39824-39835	RB	denotes	selectively
T12596	39844-39851	NNS	denotes	sterols
T12597	39852-39855	VBZ	denotes	has
T12598	39856-39859	RB	denotes	not
T12599	39860-39864	VBN	denotes	been
T12600	39875-39876	.	denotes	.
T12601	39876-40106	sentence	denotes	Identification of the genetic defect(s) in a rare human disorder, sitosterolemia, where these processes are specifically disrupted, may finally have led to the identification of the 'transporters' responsible for these processes.
T12602	39877-39891	NN	denotes	Identification
T12603	40026-40029	VBN	denotes	led
T12604	39892-39894	IN	denotes	of
T12605	39895-39898	DT	denotes	the
T12606	39907-39913	NN	denotes	defect
T12607	39899-39906	JJ	denotes	genetic
T12608	39913-39914	-LRB-	denotes	(
T12609	39914-39915	AFX	denotes	s
T12610	39915-39916	-RRB-	denotes	)
T12611	39917-39919	IN	denotes	in
T12612	39920-39921	DT	denotes	a
T12613	39933-39941	NN	denotes	disorder
T12614	39922-39926	JJ	denotes	rare
T12615	39927-39932	JJ	denotes	human
T12616	39941-39943	,	denotes	,
T12617	39943-39957	NN	denotes	sitosterolemia
T12618	39957-39959	,	denotes	,
T12619	39959-39964	WRB	denotes	where
T12620	39998-40007	VBN	denotes	disrupted
T12621	39965-39970	DT	denotes	these
T12622	39971-39980	NNS	denotes	processes
T12623	39981-39984	VBP	denotes	are
T12624	39985-39997	RB	denotes	specifically
T12625	40007-40009	,	denotes	,
T12626	40009-40012	MD	denotes	may
T12627	40013-40020	RB	denotes	finally
T12628	40021-40025	VB	denotes	have
T12629	40030-40032	IN	denotes	to
T12630	40033-40036	DT	denotes	the
T12631	40037-40051	NN	denotes	identification
T12632	40052-40054	IN	denotes	of
T12633	40055-40058	DT	denotes	the
T12634	40060-40072	NNS	denotes	transporters
T12635	40059-40060	``	denotes	'
T12636	40072-40073	''	denotes	'
T12637	40074-40085	JJ	denotes	responsible
T12638	40086-40089	IN	denotes	for
T12639	40090-40095	DT	denotes	these
T12640	40096-40105	NNS	denotes	processes
T12641	40105-40106	.	denotes	.
T12642	40106-40243	sentence	denotes	Complete defects in one of two genes (but not both), organized in a head-to-head configuration at the STSL locus, causes sitosterolemia.
T12643	40107-40115	JJ	denotes	Complete
T12644	40116-40123	NNS	denotes	defects
T12645	40221-40227	VBZ	denotes	causes
T12646	40124-40126	IN	denotes	in
T12647	40127-40130	CD	denotes	one
T12648	40134-40137	CD	denotes	two
T12649	40131-40133	IN	denotes	of
T12650	40138-40143	NNS	denotes	genes
T12651	40144-40145	-LRB-	denotes	(
T12652	40145-40148	CC	denotes	but
T12653	40149-40152	RB	denotes	not
T12654	40153-40157	DT	denotes	both
T12655	40157-40158	-RRB-	denotes	)
T12656	40158-40160	,	denotes	,
T12657	40160-40169	VBN	denotes	organized
T12658	40170-40172	IN	denotes	in
T12659	40173-40174	DT	denotes	a
T12660	40188-40201	NN	denotes	configuration
T12661	40175-40179	NN	denotes	head
T12662	40179-40180	HYPH	denotes	-
T12663	40180-40182	IN	denotes	to
T12664	40182-40183	HYPH	denotes	-
T12665	40183-40187	NN	denotes	head
T12666	40202-40204	IN	denotes	at
T12667	40205-40208	DT	denotes	the
T12668	40214-40219	NN	denotes	locus
T12669	40209-40213	NN	denotes	STSL
T12670	40219-40221	,	denotes	,
T12671	40228-40242	NN	denotes	sitosterolemia
T12672	40242-40243	.	denotes	.
T12673	40243-40355	sentence	denotes	We report a mouse model of sitosterolemia, with a selective, but complete, defect in one of these genes, Abcg8.
T12674	40244-40246	PRP	denotes	We
T12675	40247-40253	VBP	denotes	report
T12676	40254-40255	DT	denotes	a
T12677	40262-40267	NN	denotes	model
T12678	40256-40261	NN	denotes	mouse
T12679	40268-40270	IN	denotes	of
T12680	40271-40285	NN	denotes	sitosterolemia
T12681	40285-40287	,	denotes	,
T12682	40287-40291	IN	denotes	with
T12683	40292-40293	DT	denotes	a
T12684	40319-40325	NN	denotes	defect
T12685	40294-40303	JJ	denotes	selective
T12686	40303-40305	,	denotes	,
T12687	40305-40308	CC	denotes	but
T12688	40309-40317	JJ	denotes	complete
T12689	40317-40319	,	denotes	,
T12690	40326-40328	IN	denotes	in
T12691	40329-40332	CD	denotes	one
T12692	40333-40335	IN	denotes	of
T12693	40336-40341	DT	denotes	these
T12694	40342-40347	NNS	denotes	genes
T12695	40347-40349	,	denotes	,
T12696	40349-40354	NN	denotes	Abcg8
T12697	40354-40355	.	denotes	.
T12698	40355-40437	sentence	denotes	This mouse reflects the known defects described in human sitosterolemia [5,7,42].
T12699	40356-40360	DT	denotes	This
T12700	40361-40366	NN	denotes	mouse
T12701	40367-40375	VBZ	denotes	reflects
T12702	40376-40379	DT	denotes	the
T12703	40386-40393	NNS	denotes	defects
T12704	40380-40385	JJ	denotes	known
T12705	40394-40403	VBN	denotes	described
T12706	40404-40406	IN	denotes	in
T12707	40407-40412	JJ	denotes	human
T12708	40413-40427	NN	denotes	sitosterolemia
T12709	40428-40429	-LRB-	denotes	[
T12710	40433-40435	CD	denotes	42
T12711	40429-40430	CD	denotes	5
T12712	40430-40431	,	denotes	,
T12713	40431-40432	CD	denotes	7
T12714	40432-40433	,	denotes	,
T12715	40435-40436	-RRB-	denotes	]
T12716	40436-40437	.	denotes	.
T12717	40437-40530	sentence	denotes	Plasma and all tissues, apart from the brain, have significantly elevated sitosterol levels.
T12718	40438-40444	NN	denotes	Plasma
T12719	40484-40488	VBP	denotes	have
T12720	40445-40448	CC	denotes	and
T12721	40449-40452	DT	denotes	all
T12722	40453-40460	NNS	denotes	tissues
T12723	40460-40462	,	denotes	,
T12724	40462-40467	RB	denotes	apart
T12725	40468-40472	IN	denotes	from
T12726	40473-40476	DT	denotes	the
T12727	40477-40482	NN	denotes	brain
T12728	40482-40484	,	denotes	,
T12729	40489-40502	RB	denotes	significantly
T12730	40503-40511	VBN	denotes	elevated
T12731	40523-40529	NNS	denotes	levels
T12732	40512-40522	NN	denotes	sitosterol
T12733	40529-40530	.	denotes	.
T12734	40530-40595	sentence	denotes	Homozygous knockout mice are viable, fertile and sitosterolemic.
T12735	40531-40541	JJ	denotes	Homozygous
T12736	40551-40555	NNS	denotes	mice
T12737	40542-40550	NN	denotes	knockout
T12738	40556-40559	VBP	denotes	are
T12739	40560-40566	JJ	denotes	viable
T12740	40566-40568	,	denotes	,
T12741	40568-40575	JJ	denotes	fertile
T12742	40576-40579	CC	denotes	and
T12743	40580-40594	JJ	denotes	sitosterolemic
T12744	40594-40595	.	denotes	.
T12745	40595-40721	sentence	denotes	However, fertility seems to be reduced when homozygous mice are bred together (S Patel and J Oatis, unpublished observation).
T12746	40596-40603	RB	denotes	However
T12747	40615-40620	VBZ	denotes	seems
T12748	40603-40605	,	denotes	,
T12749	40605-40614	NN	denotes	fertility
T12750	40621-40623	TO	denotes	to
T12751	40627-40634	VBN	denotes	reduced
T12752	40624-40626	VB	denotes	be
T12753	40635-40639	WRB	denotes	when
T12754	40660-40664	VBN	denotes	bred
T12755	40640-40650	JJ	denotes	homozygous
T12756	40651-40655	NNS	denotes	mice
T12757	40656-40659	VBP	denotes	are
T12758	40665-40673	RB	denotes	together
T12759	40674-40675	-LRB-	denotes	(
T12760	40675-40676	NNP	denotes	S
T12761	40677-40682	NNP	denotes	Patel
T12762	40683-40686	CC	denotes	and
T12763	40687-40688	NNP	denotes	J
T12764	40689-40694	NNP	denotes	Oatis
T12765	40694-40696	,	denotes	,
T12766	40696-40707	JJ	denotes	unpublished
T12767	40708-40719	NN	denotes	observation
T12768	40719-40720	-RRB-	denotes	)
T12769	40720-40721	.	denotes	.
T12770	40721-40809	sentence	denotes	This model reflects many other observed changes described in limited studies in humans.
T12771	40722-40726	DT	denotes	This
T12772	40727-40732	NN	denotes	model
T12773	40733-40741	VBZ	denotes	reflects
T12774	40742-40746	JJ	denotes	many
T12775	40762-40769	NNS	denotes	changes
T12776	40747-40752	JJ	denotes	other
T12777	40753-40761	VBN	denotes	observed
T12778	40770-40779	VBN	denotes	described
T12779	40780-40782	IN	denotes	in
T12780	40783-40790	JJ	denotes	limited
T12781	40791-40798	NNS	denotes	studies
T12782	40799-40801	IN	denotes	in
T12783	40802-40808	NNS	denotes	humans
T12784	40808-40809	.	denotes	.
T12785	40809-40915	sentence	denotes	For example, in humans, the activity of HMG-CoA reductase and CYP7a1 have been reported to be low [7,39].
T12786	40810-40813	IN	denotes	For
T12787	40889-40897	VBN	denotes	reported
T12788	40814-40821	NN	denotes	example
T12789	40821-40823	,	denotes	,
T12790	40823-40825	IN	denotes	in
T12791	40826-40832	NNS	denotes	humans
T12792	40832-40834	,	denotes	,
T12793	40834-40837	DT	denotes	the
T12794	40838-40846	NN	denotes	activity
T12795	40847-40849	IN	denotes	of
T12796	40850-40853	NN	denotes	HMG
T12797	40854-40857	NN	denotes	CoA
T12798	40853-40854	HYPH	denotes	-
T12799	40858-40867	NN	denotes	reductase
T12800	40868-40871	CC	denotes	and
T12801	40872-40878	NN	denotes	CYP7a1
T12802	40879-40883	VBP	denotes	have
T12803	40884-40888	VBN	denotes	been
T12804	40898-40900	TO	denotes	to
T12805	40901-40903	VB	denotes	be
T12806	40904-40907	JJ	denotes	low
T12807	40908-40909	-LRB-	denotes	[
T12808	40911-40913	CD	denotes	39
T12809	40909-40910	CD	denotes	7
T12810	40910-40911	,	denotes	,
T12811	40913-40914	-RRB-	denotes	]
T12812	40914-40915	.	denotes	.
T12813	40915-41042	sentence	denotes	In this study, we show that the mRNA and enzyme activities in the livers from knockout animals are also significantly reduced.
T12814	40916-40918	IN	denotes	In
T12815	40934-40938	VBP	denotes	show
T12816	40919-40923	DT	denotes	this
T12817	40924-40929	NN	denotes	study
T12818	40929-40931	,	denotes	,
T12819	40931-40933	PRP	denotes	we
T12820	40939-40943	IN	denotes	that
T12821	41034-41041	VBN	denotes	reduced
T12822	40944-40947	DT	denotes	the
T12823	40948-40952	NN	denotes	mRNA
T12824	40953-40956	CC	denotes	and
T12825	40957-40963	NN	denotes	enzyme
T12826	40964-40974	NNS	denotes	activities
T12827	40975-40977	IN	denotes	in
T12828	40978-40981	DT	denotes	the
T12829	40982-40988	NNS	denotes	livers
T12830	40989-40993	IN	denotes	from
T12831	40994-41002	NN	denotes	knockout
T12832	41003-41010	NNS	denotes	animals
T12833	41011-41014	VBP	denotes	are
T12834	41015-41019	RB	denotes	also
T12835	41020-41033	RB	denotes	significantly
T12836	41041-41042	.	denotes	.
T12837	41042-41191	sentence	denotes	Additionally, the activity of the rate-limiting enzyme for bile acid synthesis, CYP7a1, is reduced, although no changes at the mRNA level are noted.
T12838	41043-41055	RB	denotes	Additionally
T12839	41134-41141	VBN	denotes	reduced
T12840	41055-41057	,	denotes	,
T12841	41057-41060	DT	denotes	the
T12842	41061-41069	NN	denotes	activity
T12843	41070-41072	IN	denotes	of
T12844	41073-41076	DT	denotes	the
T12845	41091-41097	NN	denotes	enzyme
T12846	41077-41081	NN	denotes	rate
T12847	41082-41090	VBG	denotes	limiting
T12848	41081-41082	HYPH	denotes	-
T12849	41098-41101	IN	denotes	for
T12850	41102-41106	NN	denotes	bile
T12851	41107-41111	NN	denotes	acid
T12852	41112-41121	NN	denotes	synthesis
T12853	41121-41123	,	denotes	,
T12854	41123-41129	NN	denotes	CYP7a1
T12855	41129-41131	,	denotes	,
T12856	41131-41133	VBZ	denotes	is
T12857	41141-41143	,	denotes	,
T12858	41143-41151	IN	denotes	although
T12859	41185-41190	VBN	denotes	noted
T12860	41152-41154	DT	denotes	no
T12861	41155-41162	NNS	denotes	changes
T12862	41163-41165	IN	denotes	at
T12863	41166-41169	DT	denotes	the
T12864	41175-41180	NN	denotes	level
T12865	41170-41174	NN	denotes	mRNA
T12866	41181-41184	VBP	denotes	are
T12867	41190-41191	.	denotes	.
T12868	41191-41315	sentence	denotes	This is also in keeping with previous studies that show that sitosterol is a direct inhibitor of this enzyme in vitro [39].
T12869	41192-41196	DT	denotes	This
T12870	41197-41199	VBZ	denotes	is
T12871	41200-41204	RB	denotes	also
T12872	41205-41207	IN	denotes	in
T12873	41208-41215	VBG	denotes	keeping
T12874	41216-41220	IN	denotes	with
T12875	41221-41229	JJ	denotes	previous
T12876	41230-41237	NNS	denotes	studies
T12877	41238-41242	WDT	denotes	that
T12878	41243-41247	VBP	denotes	show
T12879	41248-41252	IN	denotes	that
T12880	41264-41266	VBZ	denotes	is
T12881	41253-41263	NN	denotes	sitosterol
T12882	41267-41268	DT	denotes	a
T12883	41276-41285	NN	denotes	inhibitor
T12884	41269-41275	JJ	denotes	direct
T12885	41286-41288	IN	denotes	of
T12886	41289-41293	DT	denotes	this
T12887	41294-41300	NN	denotes	enzyme
T12888	41301-41303	FW	denotes	in
T12889	41304-41309	FW	denotes	vitro
T12890	41310-41311	-LRB-	denotes	[
T12891	41311-41313	CD	denotes	39
T12892	41313-41314	-RRB-	denotes	]
T12893	41314-41315	.	denotes	.
T12894	41315-41411	sentence	denotes	Biliary sterols of Abcg8-deficient mice were dramatically different compared to wild-type mice.
T12895	41316-41323	JJ	denotes	Biliary
T12896	41324-41331	NNS	denotes	sterols
T12897	41356-41360	VBD	denotes	were
T12898	41332-41334	IN	denotes	of
T12899	41335-41340	NN	denotes	Abcg8
T12900	41341-41350	JJ	denotes	deficient
T12901	41340-41341	HYPH	denotes	-
T12902	41351-41355	NNS	denotes	mice
T12903	41361-41373	RB	denotes	dramatically
T12904	41374-41383	JJ	denotes	different
T12905	41384-41392	VBN	denotes	compared
T12906	41393-41395	IN	denotes	to
T12907	41396-41400	JJ	denotes	wild
T12908	41401-41405	NN	denotes	type
T12909	41400-41401	HYPH	denotes	-
T12910	41406-41410	NNS	denotes	mice
T12911	41410-41411	.	denotes	.
T12912	41411-41578	sentence	denotes	Measurement of biliary sterol secretion rates in Abcg8-/- mice demonstrated a failure to increase sterol secretion into bile despite exogenous infusion of bile acids.
T12913	41412-41423	NN	denotes	Measurement
T12914	41475-41487	VBD	denotes	demonstrated
T12915	41424-41426	IN	denotes	of
T12916	41427-41434	JJ	denotes	biliary
T12917	41435-41441	NN	denotes	sterol
T12918	41452-41457	NNS	denotes	rates
T12919	41442-41451	NN	denotes	secretion
T12920	41458-41460	IN	denotes	in
T12921	41461-41466	NN	denotes	Abcg8
T12922	41470-41474	NNS	denotes	mice
T12923	41466-41467	SYM	denotes	-
T12924	41467-41468	HYPH	denotes	/
T12925	41468-41469	SYM	denotes	-
T12926	41488-41489	DT	denotes	a
T12927	41490-41497	NN	denotes	failure
T12928	41498-41500	TO	denotes	to
T12929	41501-41509	VB	denotes	increase
T12930	41510-41516	NN	denotes	sterol
T12931	41517-41526	NN	denotes	secretion
T12932	41527-41531	IN	denotes	into
T12933	41532-41536	NN	denotes	bile
T12934	41537-41544	IN	denotes	despite
T12935	41545-41554	JJ	denotes	exogenous
T12936	41555-41563	NN	denotes	infusion
T12937	41564-41566	IN	denotes	of
T12938	41567-41571	NN	denotes	bile
T12939	41572-41577	NNS	denotes	acids
T12940	41577-41578	.	denotes	.
T12941	41578-41740	sentence	denotes	Furthermore, complete loss of Abcg8/sterolin-2 results in an inability to secrete cholesterol into bile, although secretion of sitosterol seemed to be preserved.
T12942	41579-41590	RB	denotes	Furthermore
T12943	41626-41633	VBZ	denotes	results
T12944	41590-41592	,	denotes	,
T12945	41592-41600	JJ	denotes	complete
T12946	41601-41605	NN	denotes	loss
T12947	41606-41608	IN	denotes	of
T12948	41609-41614	NN	denotes	Abcg8
T12949	41615-41623	NN	denotes	sterolin
T12950	41614-41615	HYPH	denotes	/
T12951	41623-41624	HYPH	denotes	-
T12952	41624-41625	CD	denotes	2
T12953	41634-41636	IN	denotes	in
T12954	41637-41639	DT	denotes	an
T12955	41640-41649	NN	denotes	inability
T12956	41650-41652	TO	denotes	to
T12957	41653-41660	VB	denotes	secrete
T12958	41661-41672	NN	denotes	cholesterol
T12959	41673-41677	IN	denotes	into
T12960	41678-41682	NN	denotes	bile
T12961	41682-41684	,	denotes	,
T12962	41684-41692	IN	denotes	although
T12963	41717-41723	VBD	denotes	seemed
T12964	41693-41702	NN	denotes	secretion
T12965	41703-41705	IN	denotes	of
T12966	41706-41716	NN	denotes	sitosterol
T12967	41724-41726	TO	denotes	to
T12968	41730-41739	VBN	denotes	preserved
T12969	41727-41729	VB	denotes	be
T12970	41739-41740	.	denotes	.
T12971	41740-41936	sentence	denotes	Arguably, since the body pools and plasma sitosterol levels in the knockout mice are so considerably elevated, perhaps the biliary sitosterol levels could be considered to be inappropriately low.
T12972	41741-41749	RB	denotes	Arguably
T12973	41899-41909	VBN	denotes	considered
T12974	41749-41751	,	denotes	,
T12975	41751-41756	IN	denotes	since
T12976	41822-41825	VBP	denotes	are
T12977	41757-41760	DT	denotes	the
T12978	41766-41771	NNS	denotes	pools
T12979	41761-41765	NN	denotes	body
T12980	41772-41775	CC	denotes	and
T12981	41776-41782	NN	denotes	plasma
T12982	41783-41793	NN	denotes	sitosterol
T12983	41794-41800	NNS	denotes	levels
T12984	41801-41803	IN	denotes	in
T12985	41804-41807	DT	denotes	the
T12986	41817-41821	NNS	denotes	mice
T12987	41808-41816	NN	denotes	knockout
T12988	41826-41828	RB	denotes	so
T12989	41829-41841	RB	denotes	considerably
T12990	41842-41850	JJ	denotes	elevated
T12991	41850-41852	,	denotes	,
T12992	41852-41859	RB	denotes	perhaps
T12993	41860-41863	DT	denotes	the
T12994	41883-41889	NNS	denotes	levels
T12995	41864-41871	JJ	denotes	biliary
T12996	41872-41882	NN	denotes	sitosterol
T12997	41890-41895	MD	denotes	could
T12998	41896-41898	VB	denotes	be
T12999	41910-41912	TO	denotes	to
T13000	41913-41915	VB	denotes	be
T13001	41916-41931	RB	denotes	inappropriately
T13002	41932-41935	JJ	denotes	low
T13003	41935-41936	.	denotes	.
T13004	41936-42090	sentence	denotes	Despite this reservation, the finding that sitosterol is present in the bile suggests that plant sterols may be secreted independent of Abcg8/sterolin-2.
T13005	41937-41944	IN	denotes	Despite
T13006	42014-42022	VBZ	denotes	suggests
T13007	41945-41949	DT	denotes	this
T13008	41950-41961	NN	denotes	reservation
T13009	41961-41963	,	denotes	,
T13010	41963-41966	DT	denotes	the
T13011	41967-41974	NN	denotes	finding
T13012	41975-41979	IN	denotes	that
T13013	41991-41993	VBZ	denotes	is
T13014	41980-41990	NN	denotes	sitosterol
T13015	41994-42001	JJ	denotes	present
T13016	42002-42004	IN	denotes	in
T13017	42005-42008	DT	denotes	the
T13018	42009-42013	NN	denotes	bile
T13019	42023-42027	IN	denotes	that
T13020	42049-42057	VBN	denotes	secreted
T13021	42028-42033	NN	denotes	plant
T13022	42034-42041	NNS	denotes	sterols
T13023	42042-42045	MD	denotes	may
T13024	42046-42048	VB	denotes	be
T13025	42058-42069	RB	denotes	independent
T13026	42070-42072	IN	denotes	of
T13027	42073-42078	NN	denotes	Abcg8
T13028	42079-42087	NN	denotes	sterolin
T13029	42078-42079	HYPH	denotes	/
T13030	42087-42088	HYPH	denotes	-
T13031	42088-42089	CD	denotes	2
T13032	42089-42090	.	denotes	.
T13033	42090-42229	sentence	denotes	The ability of Abcg8-/- mice to secrete bile salt and phospholipid into bile as compared to wild-type mice was not significantly impaired.
T13034	42091-42094	DT	denotes	The
T13035	42095-42102	NN	denotes	ability
T13036	42220-42228	VBN	denotes	impaired
T13037	42103-42105	IN	denotes	of
T13038	42106-42111	NN	denotes	Abcg8
T13039	42115-42119	NNS	denotes	mice
T13040	42111-42112	SYM	denotes	-
T13041	42112-42113	HYPH	denotes	/
T13042	42113-42114	SYM	denotes	-
T13043	42120-42122	TO	denotes	to
T13044	42123-42130	VB	denotes	secrete
T13045	42131-42135	NN	denotes	bile
T13046	42136-42140	NN	denotes	salt
T13047	42141-42144	CC	denotes	and
T13048	42145-42157	NN	denotes	phospholipid
T13049	42158-42162	IN	denotes	into
T13050	42163-42167	NN	denotes	bile
T13051	42168-42170	IN	denotes	as
T13052	42171-42179	VBN	denotes	compared
T13053	42180-42182	IN	denotes	to
T13054	42183-42187	JJ	denotes	wild
T13055	42188-42192	NN	denotes	type
T13056	42187-42188	HYPH	denotes	-
T13057	42193-42197	NNS	denotes	mice
T13058	42198-42201	VBD	denotes	was
T13059	42202-42205	RB	denotes	not
T13060	42206-42219	RB	denotes	significantly
T13061	42228-42229	.	denotes	.
T13062	42229-42345	sentence	denotes	Collectively these data suggest that Abcg8/sterolin-2 is necessary for hepatobiliary cholesterol secretion in mice.
T13063	42230-42242	RB	denotes	Collectively
T13064	42254-42261	VBP	denotes	suggest
T13065	42243-42248	DT	denotes	these
T13066	42249-42253	NNS	denotes	data
T13067	42262-42266	IN	denotes	that
T13068	42284-42286	VBZ	denotes	is
T13069	42267-42272	NN	denotes	Abcg8
T13070	42273-42281	NN	denotes	sterolin
T13071	42272-42273	HYPH	denotes	/
T13072	42281-42282	HYPH	denotes	-
T13073	42282-42283	CD	denotes	2
T13074	42287-42296	JJ	denotes	necessary
T13075	42297-42300	IN	denotes	for
T13076	42301-42314	JJ	denotes	hepatobiliary
T13077	42327-42336	NN	denotes	secretion
T13078	42315-42326	NN	denotes	cholesterol
T13079	42337-42339	IN	denotes	in
T13080	42340-42344	NNS	denotes	mice
T13081	42344-42345	.	denotes	.
T13082	42345-42429	sentence	denotes	Abcg8-/- mice appeared normal and healthy maintained on a regular rodent chow diet.
T13083	42346-42351	NN	denotes	Abcg8
T13084	42355-42359	NNS	denotes	mice
T13085	42351-42352	SYM	denotes	-
T13086	42352-42353	HYPH	denotes	/
T13087	42353-42354	SYM	denotes	-
T13088	42360-42368	VBD	denotes	appeared
T13089	42369-42375	JJ	denotes	normal
T13090	42376-42379	CC	denotes	and
T13091	42380-42387	JJ	denotes	healthy
T13092	42388-42398	VBN	denotes	maintained
T13093	42399-42401	IN	denotes	on
T13094	42402-42403	DT	denotes	a
T13095	42424-42428	NN	denotes	diet
T13096	42404-42411	JJ	denotes	regular
T13097	42412-42418	NN	denotes	rodent
T13098	42419-42423	NN	denotes	chow
T13099	42428-42429	.	denotes	.
T13100	42429-42562	sentence	denotes	Their phenotypic features are very similar to those recently reported for a mouse deficient in both sterolins simultaneously [7,20].
T13101	42430-42435	PRP$	denotes	Their
T13102	42447-42455	NNS	denotes	features
T13103	42436-42446	JJ	denotes	phenotypic
T13104	42456-42459	VBP	denotes	are
T13105	42460-42464	RB	denotes	very
T13106	42465-42472	JJ	denotes	similar
T13107	42473-42475	IN	denotes	to
T13108	42476-42481	DT	denotes	those
T13109	42482-42490	RB	denotes	recently
T13110	42491-42499	VBN	denotes	reported
T13111	42500-42503	IN	denotes	for
T13112	42504-42505	DT	denotes	a
T13113	42506-42511	NN	denotes	mouse
T13114	42512-42521	JJ	denotes	deficient
T13115	42522-42524	IN	denotes	in
T13116	42525-42529	DT	denotes	both
T13117	42530-42539	NNS	denotes	sterolins
T13118	42540-42554	RB	denotes	simultaneously
T13119	42555-42556	-LRB-	denotes	[
T13120	42558-42560	CD	denotes	20
T13121	42556-42557	CD	denotes	7
T13122	42557-42558	,	denotes	,
T13123	42560-42561	-RRB-	denotes	]
T13124	42561-42562	.	denotes	.
T13125	42562-42707	sentence	denotes	Plasma and hepatic plant sterol levels in Abcg8-/- and Abcg5/Abcg8 knockout are increased similarly with marked decreases in cholesterol levels.
T13126	42563-42569	NN	denotes	Plasma
T13127	42595-42601	NNS	denotes	levels
T13128	42570-42573	CC	denotes	and
T13129	42574-42581	JJ	denotes	hepatic
T13130	42582-42587	NN	denotes	plant
T13131	42588-42594	NN	denotes	sterol
T13132	42643-42652	VBN	denotes	increased
T13133	42602-42604	IN	denotes	in
T13134	42605-42610	NN	denotes	Abcg8
T13135	42610-42611	SYM	denotes	-
T13136	42611-42612	HYPH	denotes	/
T13137	42612-42613	SYM	denotes	-
T13138	42614-42617	CC	denotes	and
T13139	42618-42623	NN	denotes	Abcg5
T13140	42624-42629	NN	denotes	Abcg8
T13141	42623-42624	HYPH	denotes	/
T13142	42630-42638	NN	denotes	knockout
T13143	42639-42642	VBP	denotes	are
T13144	42653-42662	RB	denotes	similarly
T13145	42663-42667	IN	denotes	with
T13146	42668-42674	JJ	denotes	marked
T13147	42675-42684	NNS	denotes	decreases
T13148	42685-42687	IN	denotes	in
T13149	42688-42699	NN	denotes	cholesterol
T13150	42700-42706	NNS	denotes	levels
T13151	42706-42707	.	denotes	.
T13152	42707-42905	sentence	denotes	Interestingly, plasma triglyceride levels of the Abcg8-/- mice were slightly higher than wild-type animals and this increased triglyceride is carried in the LDL fraction range, as measured by FPLC.
T13153	42708-42721	RB	denotes	Interestingly
T13154	42771-42775	VBD	denotes	were
T13155	42721-42723	,	denotes	,
T13156	42723-42729	NN	denotes	plasma
T13157	42730-42742	NN	denotes	triglyceride
T13158	42743-42749	NNS	denotes	levels
T13159	42750-42752	IN	denotes	of
T13160	42753-42756	DT	denotes	the
T13161	42766-42770	NNS	denotes	mice
T13162	42757-42762	NN	denotes	Abcg8
T13163	42764-42765	SYM	denotes	-
T13164	42762-42763	SYM	denotes	-
T13165	42763-42764	HYPH	denotes	/
T13166	42776-42784	RB	denotes	slightly
T13167	42785-42791	JJR	denotes	higher
T13168	42792-42796	IN	denotes	than
T13169	42797-42801	JJ	denotes	wild
T13170	42802-42806	NN	denotes	type
T13171	42801-42802	HYPH	denotes	-
T13172	42807-42814	NNS	denotes	animals
T13173	42815-42818	CC	denotes	and
T13174	42819-42823	DT	denotes	this
T13175	42834-42846	NN	denotes	triglyceride
T13176	42824-42833	VBN	denotes	increased
T13177	42850-42857	VBN	denotes	carried
T13178	42847-42849	VBZ	denotes	is
T13179	42858-42860	IN	denotes	in
T13180	42861-42864	DT	denotes	the
T13181	42878-42883	NN	denotes	range
T13182	42865-42868	NN	denotes	LDL
T13183	42869-42877	NN	denotes	fraction
T13184	42883-42885	,	denotes	,
T13185	42885-42887	IN	denotes	as
T13186	42888-42896	VBN	denotes	measured
T13187	42897-42899	IN	denotes	by
T13188	42900-42904	NN	denotes	FPLC
T13189	42904-42905	.	denotes	.
T13190	42905-43077	sentence	denotes	The significance of this is not clear, although preliminary SDS-PAGE analyses of all the fractions failed to reveal any differences between wild-type and knockout samples.
T13191	42906-42909	DT	denotes	The
T13192	42910-42922	NN	denotes	significance
T13193	42931-42933	VBZ	denotes	is
T13194	42923-42925	IN	denotes	of
T13195	42926-42930	DT	denotes	this
T13196	42934-42937	RB	denotes	not
T13197	42938-42943	JJ	denotes	clear
T13198	42943-42945	,	denotes	,
T13199	42945-42953	IN	denotes	although
T13200	43005-43011	VBD	denotes	failed
T13201	42954-42965	JJ	denotes	preliminary
T13202	42975-42983	NNS	denotes	analyses
T13203	42966-42969	NN	denotes	SDS
T13204	42970-42974	NN	denotes	PAGE
T13205	42969-42970	HYPH	denotes	-
T13206	42984-42986	IN	denotes	of
T13207	42987-42990	PDT	denotes	all
T13208	42995-43004	NNS	denotes	fractions
T13209	42991-42994	DT	denotes	the
T13210	43012-43014	TO	denotes	to
T13211	43015-43021	VB	denotes	reveal
T13212	43022-43025	DT	denotes	any
T13213	43026-43037	NNS	denotes	differences
T13214	43038-43045	IN	denotes	between
T13215	43046-43050	JJ	denotes	wild
T13216	43051-43055	NN	denotes	type
T13217	43050-43051	HYPH	denotes	-
T13218	43069-43076	NNS	denotes	samples
T13219	43056-43059	CC	denotes	and
T13220	43060-43068	NN	denotes	knockout
T13221	43076-43077	.	denotes	.
T13222	43077-43313	sentence	denotes	Hepatic levels of GC-measured cholesterol were greatly reduced in Abcg8-/- mice compared to wild-type mice, yet mRNA levels of HMG-CoA reductase and enzyme activity are also reduced without any significant change in mRNA of the Srebps.
T13223	43078-43085	JJ	denotes	Hepatic
T13224	43086-43092	NNS	denotes	levels
T13225	43133-43140	VBN	denotes	reduced
T13226	43093-43095	IN	denotes	of
T13227	43096-43098	NN	denotes	GC
T13228	43099-43107	VBN	denotes	measured
T13229	43098-43099	HYPH	denotes	-
T13230	43108-43119	NN	denotes	cholesterol
T13231	43120-43124	VBD	denotes	were
T13232	43125-43132	RB	denotes	greatly
T13233	43141-43143	IN	denotes	in
T13234	43144-43149	NN	denotes	Abcg8
T13235	43151-43152	SYM	denotes	-
T13236	43149-43150	SYM	denotes	-
T13237	43150-43151	HYPH	denotes	/
T13238	43153-43157	NNS	denotes	mice
T13239	43158-43166	VBN	denotes	compared
T13240	43167-43169	IN	denotes	to
T13241	43170-43174	JJ	denotes	wild
T13242	43175-43179	NN	denotes	type
T13243	43174-43175	HYPH	denotes	-
T13244	43180-43184	NNS	denotes	mice
T13245	43184-43186	,	denotes	,
T13246	43186-43189	CC	denotes	yet
T13247	43190-43194	NN	denotes	mRNA
T13248	43195-43201	NNS	denotes	levels
T13249	43252-43259	VBN	denotes	reduced
T13250	43202-43204	IN	denotes	of
T13251	43205-43208	NN	denotes	HMG
T13252	43209-43212	NN	denotes	CoA
T13253	43208-43209	HYPH	denotes	-
T13254	43213-43222	NN	denotes	reductase
T13255	43223-43226	CC	denotes	and
T13256	43227-43233	NN	denotes	enzyme
T13257	43234-43242	NN	denotes	activity
T13258	43243-43246	VBP	denotes	are
T13259	43247-43251	RB	denotes	also
T13260	43260-43267	IN	denotes	without
T13261	43268-43271	DT	denotes	any
T13262	43284-43290	NN	denotes	change
T13263	43272-43283	JJ	denotes	significant
T13264	43291-43293	IN	denotes	in
T13265	43294-43298	NN	denotes	mRNA
T13266	43299-43301	IN	denotes	of
T13267	43302-43305	DT	denotes	the
T13268	43306-43312	NNS	denotes	Srebps
T13269	43312-43313	.	denotes	.
T13270	43313-43442	sentence	denotes	The basis for this is not clear at present, unless the increase in non-plant sterols leads to a suppression of SREBP activation.
T13271	43314-43317	DT	denotes	The
T13272	43318-43323	NN	denotes	basis
T13273	43333-43335	VBZ	denotes	is
T13274	43324-43327	IN	denotes	for
T13275	43328-43332	DT	denotes	this
T13276	43336-43339	RB	denotes	not
T13277	43340-43345	JJ	denotes	clear
T13278	43346-43348	IN	denotes	at
T13279	43349-43356	JJ	denotes	present
T13280	43356-43358	,	denotes	,
T13281	43358-43364	IN	denotes	unless
T13282	43399-43404	VBZ	denotes	leads
T13283	43365-43368	DT	denotes	the
T13284	43369-43377	NN	denotes	increase
T13285	43378-43380	IN	denotes	in
T13286	43381-43390	JJ	denotes	non-plant
T13287	43391-43398	NNS	denotes	sterols
T13288	43405-43407	IN	denotes	to
T13289	43408-43409	DT	denotes	a
T13290	43410-43421	NN	denotes	suppression
T13291	43422-43424	IN	denotes	of
T13292	43425-43430	NN	denotes	SREBP
T13293	43431-43441	NN	denotes	activation
T13294	43441-43442	.	denotes	.
T13295	43442-43583	sentence	denotes	Sitosterol has been shown to directly inhibit Cyp7a1 activity in vitro and we presume this may account for the reduced enzyme activity [39].
T13296	43443-43453	NN	denotes	Sitosterol
T13297	43463-43468	VBN	denotes	shown
T13298	43454-43457	VBZ	denotes	has
T13299	43458-43462	VBN	denotes	been
T13300	43469-43471	TO	denotes	to
T13301	43481-43488	VB	denotes	inhibit
T13302	43472-43480	RB	denotes	directly
T13303	43489-43495	NN	denotes	Cyp7a1
T13304	43496-43504	NN	denotes	activity
T13305	43505-43507	FW	denotes	in
T13306	43508-43513	FW	denotes	vitro
T13307	43514-43517	CC	denotes	and
T13308	43518-43520	PRP	denotes	we
T13309	43521-43528	VBP	denotes	presume
T13310	43529-43533	DT	denotes	this
T13311	43538-43545	VB	denotes	account
T13312	43534-43537	MD	denotes	may
T13313	43546-43549	IN	denotes	for
T13314	43550-43553	DT	denotes	the
T13315	43569-43577	NN	denotes	activity
T13316	43554-43561	VBN	denotes	reduced
T13317	43562-43568	NN	denotes	enzyme
T13318	43578-43579	-LRB-	denotes	[
T13319	43579-43581	CD	denotes	39
T13320	43581-43582	-RRB-	denotes	]
T13321	43582-43583	.	denotes	.
T13322	43583-43782	sentence	denotes	In preliminary studies, when knockout mice are placed on a low sitosterol diet, the activity of this enzyme, as well as the mRNA levels are increased (E Klett and S Patel, unpublished observations).
T13323	43584-43586	IN	denotes	In
T13324	43724-43733	VBN	denotes	increased
T13325	43587-43598	JJ	denotes	preliminary
T13326	43599-43606	NNS	denotes	studies
T13327	43606-43608	,	denotes	,
T13328	43608-43612	WRB	denotes	when
T13329	43631-43637	VBN	denotes	placed
T13330	43613-43621	NN	denotes	knockout
T13331	43622-43626	NNS	denotes	mice
T13332	43627-43630	VBP	denotes	are
T13333	43638-43640	IN	denotes	on
T13334	43641-43642	DT	denotes	a
T13335	43658-43662	NN	denotes	diet
T13336	43643-43646	JJ	denotes	low
T13337	43647-43657	NN	denotes	sitosterol
T13338	43662-43664	,	denotes	,
T13339	43664-43667	DT	denotes	the
T13340	43668-43676	NN	denotes	activity
T13341	43677-43679	IN	denotes	of
T13342	43680-43684	DT	denotes	this
T13343	43685-43691	NN	denotes	enzyme
T13344	43691-43693	,	denotes	,
T13345	43693-43695	RB	denotes	as
T13346	43701-43703	IN	denotes	as
T13347	43696-43700	RB	denotes	well
T13348	43704-43707	DT	denotes	the
T13349	43713-43719	NNS	denotes	levels
T13350	43708-43712	NN	denotes	mRNA
T13351	43720-43723	VBP	denotes	are
T13352	43734-43735	-LRB-	denotes	(
T13353	43735-43736	NNP	denotes	E
T13354	43737-43742	NNP	denotes	Klett
T13355	43743-43746	CC	denotes	and
T13356	43747-43748	NNP	denotes	S
T13357	43749-43754	NNP	denotes	Patel
T13358	43754-43756	,	denotes	,
T13359	43756-43767	JJ	denotes	unpublished
T13360	43768-43780	NNS	denotes	observations
T13361	43780-43781	-RRB-	denotes	)
T13362	43781-43782	.	denotes	.
T13363	43782-44003	sentence	denotes	Another prediction, based upon the enzyme and mRNA levels in knockout mice, is that if these mice are placed on a high cholesterol/high sitosterol diet, they may show significant accumulation of both sterols in the body.
T13364	43783-43790	DT	denotes	Another
T13365	43791-43801	NN	denotes	prediction
T13366	43859-43861	VBZ	denotes	is
T13367	43801-43803	,	denotes	,
T13368	43803-43808	VBN	denotes	based
T13369	43809-43813	IN	denotes	upon
T13370	43814-43817	DT	denotes	the
T13371	43834-43840	NNS	denotes	levels
T13372	43818-43824	NN	denotes	enzyme
T13373	43825-43828	CC	denotes	and
T13374	43829-43833	NN	denotes	mRNA
T13375	43841-43843	IN	denotes	in
T13376	43844-43852	NN	denotes	knockout
T13377	43853-43857	NNS	denotes	mice
T13378	43857-43859	,	denotes	,
T13379	43862-43866	IN	denotes	that
T13380	43945-43949	VB	denotes	show
T13381	43867-43869	IN	denotes	if
T13382	43885-43891	VBN	denotes	placed
T13383	43870-43875	DT	denotes	these
T13384	43876-43880	NNS	denotes	mice
T13385	43881-43884	VBP	denotes	are
T13386	43892-43894	IN	denotes	on
T13387	43895-43896	DT	denotes	a
T13388	43930-43934	NN	denotes	diet
T13389	43897-43901	JJ	denotes	high
T13390	43902-43913	NN	denotes	cholesterol
T13391	43913-43914	HYPH	denotes	/
T13392	43914-43918	JJ	denotes	high
T13393	43919-43929	NN	denotes	sitosterol
T13394	43934-43936	,	denotes	,
T13395	43936-43940	PRP	denotes	they
T13396	43941-43944	MD	denotes	may
T13397	43950-43961	JJ	denotes	significant
T13398	43962-43974	NN	denotes	accumulation
T13399	43975-43977	IN	denotes	of
T13400	43978-43982	DT	denotes	both
T13401	43983-43990	NNS	denotes	sterols
T13402	43991-43993	IN	denotes	in
T13403	43994-43997	DT	denotes	the
T13404	43998-44002	NN	denotes	body
T13405	44002-44003	.	denotes	.
T13406	44003-44146	sentence	denotes	This may also be relevant to the human disorder where some, although not all affected individuals, manifest premature coronary artery disease.
T13407	44004-44008	DT	denotes	This
T13408	44018-44020	VB	denotes	be
T13409	44009-44012	MD	denotes	may
T13410	44013-44017	RB	denotes	also
T13411	44021-44029	JJ	denotes	relevant
T13412	44030-44032	IN	denotes	to
T13413	44033-44036	DT	denotes	the
T13414	44043-44051	NN	denotes	disorder
T13415	44037-44042	JJ	denotes	human
T13416	44052-44057	WRB	denotes	where
T13417	44103-44111	VBP	denotes	manifest
T13418	44058-44062	DT	denotes	some
T13419	44090-44101	NNS	denotes	individuals
T13420	44062-44064	,	denotes	,
T13421	44077-44080	DT	denotes	all
T13422	44064-44072	IN	denotes	although
T13423	44073-44076	RB	denotes	not
T13424	44081-44089	VBN	denotes	affected
T13425	44101-44103	,	denotes	,
T13426	44112-44121	JJ	denotes	premature
T13427	44138-44145	NN	denotes	disease
T13428	44122-44130	JJ	denotes	coronary
T13429	44131-44137	NN	denotes	artery
T13430	44145-44146	.	denotes	.
T13431	44146-44257	sentence	denotes	Abcg8/sterolin-2 deficient mice also allow us to examine the role of this protein in biliary sterol secretion.
T13432	44147-44152	NN	denotes	Abcg8
T13433	44153-44161	NN	denotes	sterolin
T13434	44152-44153	HYPH	denotes	/
T13435	44164-44173	JJ	denotes	deficient
T13436	44161-44162	HYPH	denotes	-
T13437	44162-44163	CD	denotes	2
T13438	44174-44178	NNS	denotes	mice
T13439	44184-44189	VBP	denotes	allow
T13440	44179-44183	RB	denotes	also
T13441	44190-44192	PRP	denotes	us
T13442	44196-44203	VB	denotes	examine
T13443	44193-44195	TO	denotes	to
T13444	44204-44207	DT	denotes	the
T13445	44208-44212	NN	denotes	role
T13446	44213-44215	IN	denotes	of
T13447	44216-44220	DT	denotes	this
T13448	44221-44228	NN	denotes	protein
T13449	44229-44231	IN	denotes	in
T13450	44232-44239	JJ	denotes	biliary
T13451	44240-44246	NN	denotes	sterol
T13452	44247-44256	NN	denotes	secretion
T13453	44256-44257	.	denotes	.
T13454	44257-44420	sentence	denotes	The bile is sterol poor and upon stimulation by increasing bile salt excretion, Abcg8/sterolin-2 deficient mice cannot respond, in contrast to the wild-type mice.
T13455	44258-44261	DT	denotes	The
T13456	44262-44266	NN	denotes	bile
T13457	44267-44269	VBZ	denotes	is
T13458	44270-44276	NN	denotes	sterol
T13459	44277-44281	JJ	denotes	poor
T13460	44282-44285	CC	denotes	and
T13461	44286-44290	IN	denotes	upon
T13462	44377-44384	VB	denotes	respond
T13463	44291-44302	NN	denotes	stimulation
T13464	44303-44305	IN	denotes	by
T13465	44306-44316	VBG	denotes	increasing
T13466	44317-44321	NN	denotes	bile
T13467	44322-44326	NN	denotes	salt
T13468	44327-44336	NN	denotes	excretion
T13469	44336-44338	,	denotes	,
T13470	44338-44343	NN	denotes	Abcg8
T13471	44344-44352	NN	denotes	sterolin
T13472	44343-44344	HYPH	denotes	/
T13473	44355-44364	JJ	denotes	deficient
T13474	44352-44353	HYPH	denotes	-
T13475	44353-44354	CD	denotes	2
T13476	44365-44369	NNS	denotes	mice
T13477	44370-44373	MD	denotes	can
T13478	44373-44376	RB	denotes	not
T13479	44384-44386	,	denotes	,
T13480	44386-44388	IN	denotes	in
T13481	44389-44397	NN	denotes	contrast
T13482	44398-44400	IN	denotes	to
T13483	44401-44404	DT	denotes	the
T13484	44415-44419	NNS	denotes	mice
T13485	44405-44409	JJ	denotes	wild
T13486	44410-44414	NN	denotes	type
T13487	44409-44410	HYPH	denotes	-
T13488	44419-44420	.	denotes	.
T13489	44420-44528	sentence	denotes	Thus, Abcg8/sterolin-2 is necessary for cholesterol secretion but not necessary for plant sterol secretion.
T13490	44421-44425	RB	denotes	Thus
T13491	44444-44446	VBZ	denotes	is
T13492	44425-44427	,	denotes	,
T13493	44427-44432	NN	denotes	Abcg8
T13494	44433-44441	NN	denotes	sterolin
T13495	44432-44433	HYPH	denotes	/
T13496	44441-44442	HYPH	denotes	-
T13497	44442-44443	CD	denotes	2
T13498	44447-44456	JJ	denotes	necessary
T13499	44457-44460	IN	denotes	for
T13500	44461-44472	NN	denotes	cholesterol
T13501	44473-44482	NN	denotes	secretion
T13502	44483-44486	CC	denotes	but
T13503	44487-44490	RB	denotes	not
T13504	44491-44500	JJ	denotes	necessary
T13505	44501-44504	IN	denotes	for
T13506	44505-44510	NN	denotes	plant
T13507	44511-44517	NN	denotes	sterol
T13508	44518-44527	NN	denotes	secretion
T13509	44527-44528	.	denotes	.
T13510	44528-44752	sentence	denotes	It could be argued that although the biliary sterol output is comparable to that seen in wild-type mice that this is inappropriately low, since the tissue and plasma pools of sitosterol are so elevated in the knockout mice.
T13511	44529-44531	PRP	denotes	It
T13512	44541-44547	VBN	denotes	argued
T13513	44532-44537	MD	denotes	could
T13514	44538-44540	VB	denotes	be
T13515	44548-44552	IN	denotes	that
T13516	44643-44645	VBZ	denotes	is
T13517	44553-44561	IN	denotes	although
T13518	44588-44590	VBZ	denotes	is
T13519	44562-44565	DT	denotes	the
T13520	44581-44587	NN	denotes	output
T13521	44566-44573	JJ	denotes	biliary
T13522	44574-44580	NN	denotes	sterol
T13523	44591-44601	JJ	denotes	comparable
T13524	44602-44604	IN	denotes	to
T13525	44605-44609	DT	denotes	that
T13526	44610-44614	VBN	denotes	seen
T13527	44615-44617	IN	denotes	in
T13528	44618-44622	JJ	denotes	wild
T13529	44623-44627	NN	denotes	type
T13530	44622-44623	HYPH	denotes	-
T13531	44628-44632	NNS	denotes	mice
T13532	44633-44637	IN	denotes	that
T13533	44638-44642	DT	denotes	this
T13534	44646-44661	RB	denotes	inappropriately
T13535	44662-44665	JJ	denotes	low
T13536	44665-44667	,	denotes	,
T13537	44667-44672	IN	denotes	since
T13538	44715-44718	VBP	denotes	are
T13539	44673-44676	DT	denotes	the
T13540	44695-44700	NNS	denotes	pools
T13541	44677-44683	NN	denotes	tissue
T13542	44684-44687	CC	denotes	and
T13543	44688-44694	NN	denotes	plasma
T13544	44701-44703	IN	denotes	of
T13545	44704-44714	NN	denotes	sitosterol
T13546	44719-44721	RB	denotes	so
T13547	44722-44730	JJ	denotes	elevated
T13548	44731-44733	IN	denotes	in
T13549	44734-44737	DT	denotes	the
T13550	44747-44751	NNS	denotes	mice
T13551	44738-44746	NN	denotes	knockout
T13552	44751-44752	.	denotes	.
T13553	44752-44928	sentence	denotes	Despite this caveat, the findings of sitosterol in significant quantities in the bile suggests that mechanisms other than via the sterolins can 'export' some of these sterols.
T13554	44753-44760	IN	denotes	Despite
T13555	44839-44847	VBZ	denotes	suggests
T13556	44761-44765	DT	denotes	this
T13557	44766-44772	NN	denotes	caveat
T13558	44772-44774	,	denotes	,
T13559	44774-44777	DT	denotes	the
T13560	44778-44786	NNS	denotes	findings
T13561	44787-44789	IN	denotes	of
T13562	44790-44800	NN	denotes	sitosterol
T13563	44801-44803	IN	denotes	in
T13564	44804-44815	JJ	denotes	significant
T13565	44816-44826	NNS	denotes	quantities
T13566	44827-44829	IN	denotes	in
T13567	44830-44833	DT	denotes	the
T13568	44834-44838	NN	denotes	bile
T13569	44848-44852	IN	denotes	that
T13570	44898-44904	VB	denotes	export
T13571	44853-44863	NNS	denotes	mechanisms
T13572	44864-44869	JJ	denotes	other
T13573	44870-44874	IN	denotes	than
T13574	44875-44878	IN	denotes	via
T13575	44879-44882	DT	denotes	the
T13576	44883-44892	NNS	denotes	sterolins
T13577	44893-44896	MD	denotes	can
T13578	44897-44898	``	denotes	'
T13579	44904-44905	''	denotes	'
T13580	44906-44910	DT	denotes	some
T13581	44911-44913	IN	denotes	of
T13582	44914-44919	DT	denotes	these
T13583	44920-44927	NNS	denotes	sterols
T13584	44927-44928	.	denotes	.
T13585	44928-45165	sentence	denotes	Interestingly, although humans who are heterozygous for genetic defects in either ABCG5 or ABCG8 seem to be phenotypically normal, mice heterozygous for Abcg8/sterolin-2 deficiency show that sterol secretion is impaired, but not absent.
T13586	44929-44942	RB	denotes	Interestingly
T13587	45110-45114	VBP	denotes	show
T13588	44942-44944	,	denotes	,
T13589	44944-44952	IN	denotes	although
T13590	45026-45030	VBP	denotes	seem
T13591	44953-44959	NNS	denotes	humans
T13592	44960-44963	WP	denotes	who
T13593	44964-44967	VBP	denotes	are
T13594	44968-44980	JJ	denotes	heterozygous
T13595	44981-44984	IN	denotes	for
T13596	44985-44992	JJ	denotes	genetic
T13597	44993-45000	NNS	denotes	defects
T13598	45001-45003	IN	denotes	in
T13599	45004-45010	CC	denotes	either
T13600	45011-45016	NN	denotes	ABCG5
T13601	45017-45019	CC	denotes	or
T13602	45020-45025	NN	denotes	ABCG8
T13603	45031-45033	TO	denotes	to
T13604	45034-45036	VB	denotes	be
T13605	45037-45051	RB	denotes	phenotypically
T13606	45052-45058	JJ	denotes	normal
T13607	45058-45060	,	denotes	,
T13608	45060-45064	NNS	denotes	mice
T13609	45065-45077	JJ	denotes	heterozygous
T13610	45078-45081	IN	denotes	for
T13611	45082-45087	NN	denotes	Abcg8
T13612	45088-45096	NN	denotes	sterolin
T13613	45087-45088	HYPH	denotes	/
T13614	45099-45109	NN	denotes	deficiency
T13615	45096-45097	HYPH	denotes	-
T13616	45097-45098	CD	denotes	2
T13617	45115-45119	IN	denotes	that
T13618	45137-45139	VBZ	denotes	is
T13619	45120-45126	NN	denotes	sterol
T13620	45127-45136	NN	denotes	secretion
T13621	45140-45148	JJ	denotes	impaired
T13622	45148-45150	,	denotes	,
T13623	45150-45153	CC	denotes	but
T13624	45154-45157	RB	denotes	not
T13625	45158-45164	JJ	denotes	absent
T13626	45164-45165	.	denotes	.
T13627	45165-45392	sentence	denotes	Note that under steady-state conditions on a rodent chow diet, heterozygous animals show no significant elevations in plasma or tissue sitosterol levels, suggesting that the activity of these proteins may not be rate limiting.
T13628	45166-45170	VB	denotes	Note
T13629	45171-45175	IN	denotes	that
T13630	45250-45254	VBP	denotes	show
T13631	45176-45181	IN	denotes	under
T13632	45182-45188	JJ	denotes	steady
T13633	45189-45194	NN	denotes	state
T13634	45188-45189	HYPH	denotes	-
T13635	45195-45205	NNS	denotes	conditions
T13636	45206-45208	IN	denotes	on
T13637	45209-45210	DT	denotes	a
T13638	45223-45227	NN	denotes	diet
T13639	45211-45217	NN	denotes	rodent
T13640	45218-45222	NN	denotes	chow
T13641	45227-45229	,	denotes	,
T13642	45229-45241	JJ	denotes	heterozygous
T13643	45242-45249	NNS	denotes	animals
T13644	45255-45257	DT	denotes	no
T13645	45270-45280	NNS	denotes	elevations
T13646	45258-45269	JJ	denotes	significant
T13647	45281-45283	IN	denotes	in
T13648	45284-45290	NN	denotes	plasma
T13649	45301-45311	NN	denotes	sitosterol
T13650	45291-45293	CC	denotes	or
T13651	45294-45300	NN	denotes	tissue
T13652	45312-45318	NNS	denotes	levels
T13653	45318-45320	,	denotes	,
T13654	45320-45330	VBG	denotes	suggesting
T13655	45331-45335	IN	denotes	that
T13656	45375-45377	VB	denotes	be
T13657	45336-45339	DT	denotes	the
T13658	45340-45348	NN	denotes	activity
T13659	45349-45351	IN	denotes	of
T13660	45352-45357	DT	denotes	these
T13661	45358-45366	NN	denotes	proteins
T13662	45367-45370	MD	denotes	may
T13663	45371-45374	RB	denotes	not
T13664	45378-45382	NN	denotes	rate
T13665	45383-45391	VBG	denotes	limiting
T13666	45391-45392	.	denotes	.
T13667	45392-45511	sentence	denotes	Furthermore, the heterozygous Abcg8+/- mice showed higher levels of biliary sitosterol relative to the wild-type mice.
T13668	45393-45404	RB	denotes	Furthermore
T13669	45437-45443	VBD	denotes	showed
T13670	45404-45406	,	denotes	,
T13671	45406-45409	DT	denotes	the
T13672	45432-45436	NNS	denotes	mice
T13673	45410-45422	JJ	denotes	heterozygous
T13674	45423-45428	NN	denotes	Abcg8
T13675	45428-45429	SYM	denotes	+
T13676	45429-45430	HYPH	denotes	/
T13677	45430-45431	SYM	denotes	-
T13678	45444-45450	JJR	denotes	higher
T13679	45451-45457	NNS	denotes	levels
T13680	45458-45460	IN	denotes	of
T13681	45461-45468	JJ	denotes	biliary
T13682	45469-45479	NN	denotes	sitosterol
T13683	45480-45488	JJ	denotes	relative
T13684	45489-45491	IN	denotes	to
T13685	45492-45495	DT	denotes	the
T13686	45506-45510	NNS	denotes	mice
T13687	45496-45500	JJ	denotes	wild
T13688	45501-45505	NN	denotes	type
T13689	45500-45501	HYPH	denotes	-
T13690	45510-45511	.	denotes	.
T13691	45511-45773	sentence	denotes	While the rate of total biliary sterol secretion is reduced compared to wild-type mice, since the heterozygous mice are not sitosterolemic, either the activity of these proteins is not rate limiting or other mechanisms can compensate for a 50% loss of activity.
T13692	45512-45517	IN	denotes	While
T13693	45564-45571	VBN	denotes	reduced
T13694	45518-45521	DT	denotes	the
T13695	45522-45526	NN	denotes	rate
T13696	45527-45529	IN	denotes	of
T13697	45530-45535	JJ	denotes	total
T13698	45551-45560	NN	denotes	secretion
T13699	45536-45543	JJ	denotes	biliary
T13700	45544-45550	NN	denotes	sterol
T13701	45561-45563	VBZ	denotes	is
T13702	45690-45692	VBZ	denotes	is
T13703	45572-45580	VBN	denotes	compared
T13704	45581-45583	IN	denotes	to
T13705	45584-45588	JJ	denotes	wild
T13706	45589-45593	NN	denotes	type
T13707	45588-45589	HYPH	denotes	-
T13708	45594-45598	NNS	denotes	mice
T13709	45598-45600	,	denotes	,
T13710	45600-45605	IN	denotes	since
T13711	45628-45631	VBP	denotes	are
T13712	45606-45609	DT	denotes	the
T13713	45623-45627	NNS	denotes	mice
T13714	45610-45622	JJ	denotes	heterozygous
T13715	45632-45635	RB	denotes	not
T13716	45636-45650	JJ	denotes	sitosterolemic
T13717	45650-45652	,	denotes	,
T13718	45652-45658	CC	denotes	either
T13719	45659-45662	DT	denotes	the
T13720	45663-45671	NN	denotes	activity
T13721	45672-45674	IN	denotes	of
T13722	45675-45680	DT	denotes	these
T13723	45681-45689	NN	denotes	proteins
T13724	45693-45696	RB	denotes	not
T13725	45697-45701	NN	denotes	rate
T13726	45702-45710	VBG	denotes	limiting
T13727	45711-45713	CC	denotes	or
T13728	45714-45719	JJ	denotes	other
T13729	45720-45730	NNS	denotes	mechanisms
T13730	45735-45745	VB	denotes	compensate
T13731	45731-45734	MD	denotes	can
T13732	45746-45749	IN	denotes	for
T13733	45750-45751	DT	denotes	a
T13734	45756-45760	NN	denotes	loss
T13735	45752-45754	CD	denotes	50
T13736	45754-45755	NN	denotes	%
T13737	45761-45763	IN	denotes	of
T13738	45764-45772	NN	denotes	activity
T13739	45772-45773	.	denotes	.
T13740	45773-45999	sentence	denotes	On the other hand, since feeding is intermittent, a slow, but continuous secretion during post-prandial periods could easily negate any mild temporary increase in tissue and plasma plant sterol levels in heterozygous animals.
T13741	45774-45776	IN	denotes	On
T13742	45899-45905	VB	denotes	negate
T13743	45777-45780	DT	denotes	the
T13744	45787-45791	NN	denotes	hand
T13745	45781-45786	JJ	denotes	other
T13746	45791-45793	,	denotes	,
T13747	45793-45798	IN	denotes	since
T13748	45807-45809	VBZ	denotes	is
T13749	45799-45806	NN	denotes	feeding
T13750	45810-45822	JJ	denotes	intermittent
T13751	45822-45824	,	denotes	,
T13752	45824-45825	DT	denotes	a
T13753	45847-45856	NN	denotes	secretion
T13754	45826-45830	JJ	denotes	slow
T13755	45830-45832	,	denotes	,
T13756	45832-45835	CC	denotes	but
T13757	45836-45846	JJ	denotes	continuous
T13758	45857-45863	IN	denotes	during
T13759	45864-45877	JJ	denotes	post-prandial
T13760	45878-45885	NNS	denotes	periods
T13761	45886-45891	MD	denotes	could
T13762	45892-45898	RB	denotes	easily
T13763	45906-45909	DT	denotes	any
T13764	45925-45933	NN	denotes	increase
T13765	45910-45914	JJ	denotes	mild
T13766	45915-45924	JJ	denotes	temporary
T13767	45934-45936	IN	denotes	in
T13768	45937-45943	NN	denotes	tissue
T13769	45968-45974	NNS	denotes	levels
T13770	45944-45947	CC	denotes	and
T13771	45948-45954	NN	denotes	plasma
T13772	45955-45960	NN	denotes	plant
T13773	45961-45967	NN	denotes	sterol
T13774	45975-45977	IN	denotes	in
T13775	45978-45990	JJ	denotes	heterozygous
T13776	45991-45998	NNS	denotes	animals
T13777	45998-45999	.	denotes	.
T13778	45999-46124	sentence	denotes	This may be amenable to testing by placing these animals on a high plant sterol diet and measuring the plasma sterol levels.
T13779	46000-46004	DT	denotes	This
T13780	46009-46011	VB	denotes	be
T13781	46005-46008	MD	denotes	may
T13782	46012-46020	JJ	denotes	amenable
T13783	46021-46023	IN	denotes	to
T13784	46024-46031	VBG	denotes	testing
T13785	46032-46034	IN	denotes	by
T13786	46035-46042	VBG	denotes	placing
T13787	46043-46048	DT	denotes	these
T13788	46049-46056	NNS	denotes	animals
T13789	46057-46059	IN	denotes	on
T13790	46060-46061	DT	denotes	a
T13791	46080-46084	NN	denotes	diet
T13792	46062-46066	JJ	denotes	high
T13793	46073-46079	NN	denotes	sterol
T13794	46067-46072	NN	denotes	plant
T13795	46085-46088	CC	denotes	and
T13796	46089-46098	VBG	denotes	measuring
T13797	46099-46102	DT	denotes	the
T13798	46117-46123	NNS	denotes	levels
T13799	46103-46109	NN	denotes	plasma
T13800	46110-46116	NN	denotes	sterol
T13801	46123-46124	.	denotes	.
T13802	46124-46289	sentence	denotes	Recently, Kosters et al. reported that Abcg5/Abcg8 mRNA expression in a variety of different mouse strains correlated with biliary cholesterol secretion rates [30].
T13803	46125-46133	RB	denotes	Recently
T13804	46150-46158	VBD	denotes	reported
T13805	46133-46135	,	denotes	,
T13806	46135-46142	NNP	denotes	Kosters
T13807	46143-46145	FW	denotes	et
T13808	46146-46149	FW	denotes	al.
T13809	46159-46163	IN	denotes	that
T13810	46232-46242	VBD	denotes	correlated
T13811	46164-46169	NN	denotes	Abcg5
T13812	46170-46175	NN	denotes	Abcg8
T13813	46169-46170	HYPH	denotes	/
T13814	46176-46180	NN	denotes	mRNA
T13815	46181-46191	NN	denotes	expression
T13816	46192-46194	IN	denotes	in
T13817	46195-46196	DT	denotes	a
T13818	46197-46204	NN	denotes	variety
T13819	46205-46207	IN	denotes	of
T13820	46208-46217	JJ	denotes	different
T13821	46224-46231	NNS	denotes	strains
T13822	46218-46223	NN	denotes	mouse
T13823	46243-46247	IN	denotes	with
T13824	46248-46255	JJ	denotes	biliary
T13825	46256-46267	NN	denotes	cholesterol
T13826	46268-46277	NN	denotes	secretion
T13827	46278-46283	NNS	denotes	rates
T13828	46284-46285	-LRB-	denotes	[
T13829	46285-46287	CD	denotes	30
T13830	46287-46288	-RRB-	denotes	]
T13831	46288-46289	.	denotes	.
T13832	46289-46440	sentence	denotes	In their studies, although diosgenin-fed mice showed a marked increase in biliary cholesterol output, mRNA levels of Abcg5 and Abcg8 were not altered.
T13833	46290-46292	IN	denotes	In
T13834	46432-46439	VBN	denotes	altered
T13835	46293-46298	PRP$	denotes	their
T13836	46299-46306	NNS	denotes	studies
T13837	46306-46308	,	denotes	,
T13838	46308-46316	IN	denotes	although
T13839	46336-46342	VBD	denotes	showed
T13840	46317-46326	NN	denotes	diosgenin
T13841	46327-46330	VBN	denotes	fed
T13842	46326-46327	HYPH	denotes	-
T13843	46331-46335	NNS	denotes	mice
T13844	46343-46344	DT	denotes	a
T13845	46352-46360	NN	denotes	increase
T13846	46345-46351	JJ	denotes	marked
T13847	46361-46363	IN	denotes	in
T13848	46364-46371	JJ	denotes	biliary
T13849	46384-46390	NN	denotes	output
T13850	46372-46383	NN	denotes	cholesterol
T13851	46390-46392	,	denotes	,
T13852	46392-46396	NN	denotes	mRNA
T13853	46397-46403	NNS	denotes	levels
T13854	46404-46406	IN	denotes	of
T13855	46407-46412	NN	denotes	Abcg5
T13856	46413-46416	CC	denotes	and
T13857	46417-46422	NN	denotes	Abcg8
T13858	46423-46427	VBD	denotes	were
T13859	46428-46431	RB	denotes	not
T13860	46439-46440	.	denotes	.
T13861	46440-46564	sentence	denotes	Using Western blot analyses, the protein level of Abcg5 was also not altered by diosgenin, although Abcg8 was not measured.
T13862	46441-46446	VBG	denotes	Using
T13863	46510-46517	VBN	denotes	altered
T13864	46447-46454	NNP	denotes	Western
T13865	46455-46459	NN	denotes	blot
T13866	46460-46468	NNS	denotes	analyses
T13867	46468-46470	,	denotes	,
T13868	46470-46473	DT	denotes	the
T13869	46482-46487	NN	denotes	level
T13870	46474-46481	NN	denotes	protein
T13871	46488-46490	IN	denotes	of
T13872	46491-46496	NN	denotes	Abcg5
T13873	46497-46500	VBD	denotes	was
T13874	46501-46505	RB	denotes	also
T13875	46506-46509	RB	denotes	not
T13876	46518-46520	IN	denotes	by
T13877	46521-46530	NN	denotes	diosgenin
T13878	46530-46532	,	denotes	,
T13879	46532-46540	IN	denotes	although
T13880	46555-46563	VBN	denotes	measured
T13881	46541-46546	NN	denotes	Abcg8
T13882	46547-46550	VBD	denotes	was
T13883	46551-46554	RB	denotes	not
T13884	46563-46564	.	denotes	.
T13885	46564-46687	sentence	denotes	They concluded that a parallel route for biliary cholesterol secretion might be operational, independent of the sterolins.
T13886	46565-46569	PRP	denotes	They
T13887	46570-46579	VBD	denotes	concluded
T13888	46580-46584	IN	denotes	that
T13889	46642-46644	VB	denotes	be
T13890	46585-46586	DT	denotes	a
T13891	46596-46601	NN	denotes	route
T13892	46587-46595	JJ	denotes	parallel
T13893	46602-46605	IN	denotes	for
T13894	46606-46613	JJ	denotes	biliary
T13895	46614-46625	NN	denotes	cholesterol
T13896	46626-46635	NN	denotes	secretion
T13897	46636-46641	MD	denotes	might
T13898	46645-46656	JJ	denotes	operational
T13899	46656-46658	,	denotes	,
T13900	46658-46669	JJ	denotes	independent
T13901	46670-46672	IN	denotes	of
T13902	46673-46676	DT	denotes	the
T13903	46677-46686	NNS	denotes	sterolins
T13904	46686-46687	.	denotes	.
T13905	46687-46844	sentence	denotes	While biliary cholesterol secretion is not completely absent in the Abcg8-deficient mice it would appear that sterolin-2 plays a major role in this process.
T13906	46688-46693	IN	denotes	While
T13907	46724-46726	VBZ	denotes	is
T13908	46694-46701	JJ	denotes	biliary
T13909	46702-46713	NN	denotes	cholesterol
T13910	46714-46723	NN	denotes	secretion
T13911	46786-46792	VB	denotes	appear
T13912	46727-46730	RB	denotes	not
T13913	46731-46741	RB	denotes	completely
T13914	46742-46748	JJ	denotes	absent
T13915	46749-46751	IN	denotes	in
T13916	46752-46755	DT	denotes	the
T13917	46772-46776	NNS	denotes	mice
T13918	46756-46761	NN	denotes	Abcg8
T13919	46762-46771	JJ	denotes	deficient
T13920	46761-46762	HYPH	denotes	-
T13921	46777-46779	PRP	denotes	it
T13922	46780-46785	MD	denotes	would
T13923	46793-46797	IN	denotes	that
T13924	46809-46814	VBZ	denotes	plays
T13925	46798-46806	NN	denotes	sterolin
T13926	46806-46807	HYPH	denotes	-
T13927	46807-46808	CD	denotes	2
T13928	46815-46816	DT	denotes	a
T13929	46823-46827	NN	denotes	role
T13930	46817-46822	JJ	denotes	major
T13931	46828-46830	IN	denotes	in
T13932	46831-46835	DT	denotes	this
T13933	46836-46843	NN	denotes	process
T13934	46843-46844	.	denotes	.
T13935	46844-47064	sentence	denotes	In a genetic screen of plasma plant sterol levels, Sehayek et al. identified three loci that may be responsible for controlling plasma plant sterol levels and not one of these loci mapped to the murine STSL region [43].
T13936	46845-46847	IN	denotes	In
T13937	46911-46921	VBD	denotes	identified
T13938	46848-46849	DT	denotes	a
T13939	46858-46864	NN	denotes	screen
T13940	46850-46857	JJ	denotes	genetic
T13941	46865-46867	IN	denotes	of
T13942	46868-46874	NN	denotes	plasma
T13943	46888-46894	NNS	denotes	levels
T13944	46875-46880	NN	denotes	plant
T13945	46881-46887	NN	denotes	sterol
T13946	46894-46896	,	denotes	,
T13947	46896-46903	NNP	denotes	Sehayek
T13948	46904-46906	FW	denotes	et
T13949	46907-46910	FW	denotes	al.
T13950	46922-46927	CD	denotes	three
T13951	46928-46932	NNS	denotes	loci
T13952	46933-46937	WDT	denotes	that
T13953	46942-46944	VB	denotes	be
T13954	46938-46941	MD	denotes	may
T13955	46945-46956	JJ	denotes	responsible
T13956	46957-46960	IN	denotes	for
T13957	46961-46972	VBG	denotes	controlling
T13958	46973-46979	NN	denotes	plasma
T13959	46993-46999	NNS	denotes	levels
T13960	46980-46985	NN	denotes	plant
T13961	46986-46992	NN	denotes	sterol
T13962	47000-47003	CC	denotes	and
T13963	47004-47007	RB	denotes	not
T13964	47008-47011	CD	denotes	one
T13965	47026-47032	VBN	denotes	mapped
T13966	47012-47014	IN	denotes	of
T13967	47015-47020	DT	denotes	these
T13968	47021-47025	NNS	denotes	loci
T13969	47033-47035	IN	denotes	to
T13970	47036-47039	DT	denotes	the
T13971	47052-47058	NN	denotes	region
T13972	47040-47046	JJ	denotes	murine
T13973	47047-47051	NN	denotes	STSL
T13974	47059-47060	-LRB-	denotes	[
T13975	47060-47062	CD	denotes	43
T13976	47062-47063	-RRB-	denotes	]
T13977	47063-47064	.	denotes	.
T13978	47064-47173	sentence	denotes	Thus, there is support for at least four loci that may be involved in regulating plasma plant sterol levels.
T13979	47065-47069	RB	denotes	Thus
T13980	47077-47079	VBZ	denotes	is
T13981	47069-47071	,	denotes	,
T13982	47071-47076	EX	denotes	there
T13983	47080-47087	NN	denotes	support
T13984	47088-47091	IN	denotes	for
T13985	47092-47094	RB	denotes	at
T13986	47101-47105	CD	denotes	four
T13987	47095-47100	RBS	denotes	least
T13988	47106-47110	NNS	denotes	loci
T13989	47111-47115	WDT	denotes	that
T13990	47123-47131	VBN	denotes	involved
T13991	47116-47119	MD	denotes	may
T13992	47120-47122	VB	denotes	be
T13993	47132-47134	IN	denotes	in
T13994	47135-47145	VBG	denotes	regulating
T13995	47146-47152	NN	denotes	plasma
T13996	47166-47172	NNS	denotes	levels
T13997	47153-47158	NN	denotes	plant
T13998	47159-47165	NN	denotes	sterol
T13999	47172-47173	.	denotes	.
T14000	47173-47321	sentence	denotes	To date the STSL locus is the only one proven to be involved in dietary sterol trafficking and the identity of the others remains to be elucidated.
T14001	47174-47176	IN	denotes	To
T14002	47197-47199	VBZ	denotes	is
T14003	47177-47181	NN	denotes	date
T14004	47182-47185	DT	denotes	the
T14005	47191-47196	NN	denotes	locus
T14006	47186-47190	NN	denotes	STSL
T14007	47200-47203	DT	denotes	the
T14008	47209-47212	CD	denotes	one
T14009	47204-47208	JJ	denotes	only
T14010	47213-47219	VBN	denotes	proven
T14011	47220-47222	TO	denotes	to
T14012	47226-47234	VBN	denotes	involved
T14013	47223-47225	VB	denotes	be
T14014	47235-47237	IN	denotes	in
T14015	47238-47245	JJ	denotes	dietary
T14016	47246-47252	NN	denotes	sterol
T14017	47253-47264	NN	denotes	trafficking
T14018	47265-47268	CC	denotes	and
T14019	47269-47272	DT	denotes	the
T14020	47273-47281	NN	denotes	identity
T14021	47296-47303	VBZ	denotes	remains
T14022	47282-47284	IN	denotes	of
T14023	47285-47288	DT	denotes	the
T14024	47289-47295	NNS	denotes	others
T14025	47304-47306	TO	denotes	to
T14026	47310-47320	VBN	denotes	elucidated
T14027	47307-47309	VB	denotes	be
T14028	47320-47321	.	denotes	.
T14029	47321-47434	sentence	denotes	We, as well as others, have proposed Abcg5/sterolin-1 and Abcg8/sterolin-2 to function as obligate heterodimers.
T14030	47322-47324	PRP	denotes	We
T14031	47350-47358	VBN	denotes	proposed
T14032	47324-47326	,	denotes	,
T14033	47326-47328	RB	denotes	as
T14034	47334-47336	IN	denotes	as
T14035	47329-47333	RB	denotes	well
T14036	47337-47343	NNS	denotes	others
T14037	47343-47345	,	denotes	,
T14038	47345-47349	VBP	denotes	have
T14039	47359-47364	NN	denotes	Abcg5
T14040	47365-47373	NN	denotes	sterolin
T14041	47364-47365	HYPH	denotes	/
T14042	47373-47374	HYPH	denotes	-
T14043	47374-47375	CD	denotes	1
T14044	47376-47379	CC	denotes	and
T14045	47380-47385	NN	denotes	Abcg8
T14046	47386-47394	NN	denotes	sterolin
T14047	47385-47386	HYPH	denotes	/
T14048	47394-47395	HYPH	denotes	-
T14049	47395-47396	CD	denotes	2
T14050	47397-47399	TO	denotes	to
T14051	47400-47408	VB	denotes	function
T14052	47409-47411	IN	denotes	as
T14053	47412-47420	JJ	denotes	obligate
T14054	47421-47433	NNS	denotes	heterodimers
T14055	47433-47434	.	denotes	.
T14056	47434-47529	sentence	denotes	Thus knocking out Abcg8/sterolin-2 alone is equivalent to a functional loss of both sterolins.
T14057	47435-47439	RB	denotes	Thus
T14058	47476-47478	VBZ	denotes	is
T14059	47440-47448	VBG	denotes	knocking
T14060	47449-47452	RP	denotes	out
T14061	47453-47458	NN	denotes	Abcg8
T14062	47459-47467	NN	denotes	sterolin
T14063	47458-47459	HYPH	denotes	/
T14064	47467-47468	HYPH	denotes	-
T14065	47468-47469	CD	denotes	2
T14066	47470-47475	RB	denotes	alone
T14067	47479-47489	JJ	denotes	equivalent
T14068	47490-47492	IN	denotes	to
T14069	47493-47494	DT	denotes	a
T14070	47506-47510	NN	denotes	loss
T14071	47495-47505	JJ	denotes	functional
T14072	47511-47513	IN	denotes	of
T14073	47514-47518	DT	denotes	both
T14074	47519-47528	NNS	denotes	sterolins
T14075	47528-47529	.	denotes	.
T14076	47529-47756	sentence	denotes	It has been shown that Abcg8/sterolin-2 expression is required for correct apical trafficking of Abcg5/sterolin-1 to the apical surface in a polarized cell line and more recently in vivo by over-expression experiments [18,19].
T14077	47530-47532	PRP	denotes	It
T14078	47542-47547	VBN	denotes	shown
T14079	47533-47536	VBZ	denotes	has
T14080	47537-47541	VBN	denotes	been
T14081	47548-47552	IN	denotes	that
T14082	47584-47592	VBN	denotes	required
T14083	47553-47558	NN	denotes	Abcg8
T14084	47559-47567	NN	denotes	sterolin
T14085	47558-47559	HYPH	denotes	/
T14086	47570-47580	NN	denotes	expression
T14087	47567-47568	HYPH	denotes	-
T14088	47568-47569	CD	denotes	2
T14089	47581-47583	VBZ	denotes	is
T14090	47593-47596	IN	denotes	for
T14091	47597-47604	JJ	denotes	correct
T14092	47612-47623	NN	denotes	trafficking
T14093	47605-47611	JJ	denotes	apical
T14094	47624-47626	IN	denotes	of
T14095	47627-47632	NN	denotes	Abcg5
T14096	47633-47641	NN	denotes	sterolin
T14097	47632-47633	HYPH	denotes	/
T14098	47641-47642	HYPH	denotes	-
T14099	47642-47643	CD	denotes	1
T14100	47644-47646	IN	denotes	to
T14101	47647-47650	DT	denotes	the
T14102	47658-47665	NN	denotes	surface
T14103	47651-47657	JJ	denotes	apical
T14104	47666-47668	IN	denotes	in
T14105	47669-47670	DT	denotes	a
T14106	47686-47690	NN	denotes	line
T14107	47671-47680	VBN	denotes	polarized
T14108	47681-47685	NN	denotes	cell
T14109	47691-47694	CC	denotes	and
T14110	47695-47699	RBR	denotes	more
T14111	47700-47708	RB	denotes	recently
T14112	47712-47716	FW	denotes	vivo
T14113	47709-47711	FW	denotes	in
T14114	47717-47719	IN	denotes	by
T14115	47720-47735	NN	denotes	over-expression
T14116	47736-47747	NNS	denotes	experiments
T14117	47748-47749	-LRB-	denotes	[
T14118	47752-47754	CD	denotes	19
T14119	47749-47751	CD	denotes	18
T14120	47751-47752	,	denotes	,
T14121	47754-47755	-RRB-	denotes	]
T14122	47755-47756	.	denotes	.
T14123	47756-47949	sentence	denotes	In experiments shown here, using three separately developed anti-Abcg5/sterolin-1 antibodies, we have obtained inconsistent data regarding the trafficking of Abcg5/sterolin-1 in Abcg8-/- mice.
T14124	47757-47759	IN	denotes	In
T14125	47859-47867	VBN	denotes	obtained
T14126	47760-47771	NNS	denotes	experiments
T14127	47772-47777	VBN	denotes	shown
T14128	47778-47782	RB	denotes	here
T14129	47782-47784	,	denotes	,
T14130	47784-47789	VBG	denotes	using
T14131	47790-47795	CD	denotes	three
T14132	47839-47849	NNS	denotes	antibodies
T14133	47796-47806	RB	denotes	separately
T14134	47807-47816	VBN	denotes	developed
T14135	47817-47827	NN	denotes	anti-Abcg5
T14136	47828-47836	NN	denotes	sterolin
T14137	47827-47828	HYPH	denotes	/
T14138	47836-47837	HYPH	denotes	-
T14139	47837-47838	CD	denotes	1
T14140	47849-47851	,	denotes	,
T14141	47851-47853	PRP	denotes	we
T14142	47854-47858	VBP	denotes	have
T14143	47868-47880	JJ	denotes	inconsistent
T14144	47881-47885	NNS	denotes	data
T14145	47886-47895	VBG	denotes	regarding
T14146	47896-47899	DT	denotes	the
T14147	47900-47911	NN	denotes	trafficking
T14148	47912-47914	IN	denotes	of
T14149	47915-47920	NN	denotes	Abcg5
T14150	47921-47929	NN	denotes	sterolin
T14151	47920-47921	HYPH	denotes	/
T14152	47929-47930	HYPH	denotes	-
T14153	47930-47931	CD	denotes	1
T14154	47932-47934	IN	denotes	in
T14155	47935-47940	NN	denotes	Abcg8
T14156	47944-47948	NNS	denotes	mice
T14157	47940-47941	SYM	denotes	-
T14158	47941-47942	HYPH	denotes	/
T14159	47942-47943	SYM	denotes	-
T14160	47948-47949	.	denotes	.
T14161	47949-48099	sentence	denotes	Based upon classical N-glycosylation maturation it would appear that Abcg5/sterolin-1, in the Abcg8-/- mice, does not exit the endoplasmic reticulum.
T14162	47950-47955	VBN	denotes	Based
T14163	48007-48013	VB	denotes	appear
T14164	47956-47960	IN	denotes	upon
T14165	47961-47970	JJ	denotes	classical
T14166	47987-47997	NN	denotes	maturation
T14167	47971-47972	NN	denotes	N
T14168	47973-47986	NN	denotes	glycosylation
T14169	47972-47973	HYPH	denotes	-
T14170	47998-48000	PRP	denotes	it
T14171	48001-48006	MD	denotes	would
T14172	48014-48018	IN	denotes	that
T14173	48068-48072	VB	denotes	exit
T14174	48019-48024	NN	denotes	Abcg5
T14175	48025-48033	NN	denotes	sterolin
T14176	48024-48025	HYPH	denotes	/
T14177	48033-48034	HYPH	denotes	-
T14178	48034-48035	CD	denotes	1
T14179	48035-48037	,	denotes	,
T14180	48037-48039	IN	denotes	in
T14181	48040-48043	DT	denotes	the
T14182	48053-48057	NNS	denotes	mice
T14183	48044-48049	NN	denotes	Abcg8
T14184	48051-48052	SYM	denotes	-
T14185	48049-48050	SYM	denotes	-
T14186	48050-48051	HYPH	denotes	/
T14187	48057-48059	,	denotes	,
T14188	48059-48063	VBZ	denotes	does
T14189	48064-48067	RB	denotes	not
T14190	48073-48076	DT	denotes	the
T14191	48089-48098	NN	denotes	reticulum
T14192	48077-48088	JJ	denotes	endoplasmic
T14193	48098-48099	.	denotes	.
T14194	48099-48270	sentence	denotes	However, by immunohistochemistry it appears that Abcg5/sterolin-1 is apically expressed in the Abcg8-deficient mice regardless of the anti-Abcg5/sterolin-1 antibody used.
T14195	48100-48107	RB	denotes	However
T14196	48136-48143	VBZ	denotes	appears
T14197	48107-48109	,	denotes	,
T14198	48109-48111	IN	denotes	by
T14199	48112-48132	NN	denotes	immunohistochemistry
T14200	48133-48135	PRP	denotes	it
T14201	48144-48148	IN	denotes	that
T14202	48178-48187	VBN	denotes	expressed
T14203	48149-48154	NN	denotes	Abcg5
T14204	48155-48163	NN	denotes	sterolin
T14205	48154-48155	HYPH	denotes	/
T14206	48163-48164	HYPH	denotes	-
T14207	48164-48165	CD	denotes	1
T14208	48166-48168	VBZ	denotes	is
T14209	48169-48177	RB	denotes	apically
T14210	48188-48190	IN	denotes	in
T14211	48191-48194	DT	denotes	the
T14212	48211-48215	NNS	denotes	mice
T14213	48195-48200	NN	denotes	Abcg8
T14214	48201-48210	JJ	denotes	deficient
T14215	48200-48201	HYPH	denotes	-
T14216	48216-48226	RB	denotes	regardless
T14217	48227-48229	IN	denotes	of
T14218	48230-48233	DT	denotes	the
T14219	48256-48264	NN	denotes	antibody
T14220	48234-48244	NN	denotes	anti-Abcg5
T14221	48245-48253	NN	denotes	sterolin
T14222	48244-48245	HYPH	denotes	/
T14223	48253-48254	HYPH	denotes	-
T14224	48254-48255	CD	denotes	1
T14225	48265-48269	VBN	denotes	used
T14226	48269-48270	.	denotes	.
T14227	48270-48443	sentence	denotes	Interestingly, in a report of Abcg5/Abcg8 localization in canine gallbladder epithelial cells, these two proteins were found intracellularly under baseline conditions [44].
T14228	48271-48284	RB	denotes	Interestingly
T14229	48390-48395	VBN	denotes	found
T14230	48284-48286	,	denotes	,
T14231	48286-48288	IN	denotes	in
T14232	48289-48290	DT	denotes	a
T14233	48291-48297	NN	denotes	report
T14234	48298-48300	IN	denotes	of
T14235	48301-48306	NN	denotes	Abcg5
T14236	48307-48312	NN	denotes	Abcg8
T14237	48306-48307	HYPH	denotes	/
T14238	48313-48325	NN	denotes	localization
T14239	48326-48328	IN	denotes	in
T14240	48329-48335	JJ	denotes	canine
T14241	48336-48347	NN	denotes	gallbladder
T14242	48359-48364	NNS	denotes	cells
T14243	48348-48358	JJ	denotes	epithelial
T14244	48364-48366	,	denotes	,
T14245	48366-48371	DT	denotes	these
T14246	48376-48384	NN	denotes	proteins
T14247	48372-48375	CD	denotes	two
T14248	48385-48389	VBD	denotes	were
T14249	48396-48411	RB	denotes	intracellularly
T14250	48412-48417	IN	denotes	under
T14251	48418-48426	NN	denotes	baseline
T14252	48427-48437	NNS	denotes	conditions
T14253	48438-48439	-LRB-	denotes	[
T14254	48439-48441	CD	denotes	44
T14255	48441-48442	-RRB-	denotes	]
T14256	48442-48443	.	denotes	.
T14257	48443-48583	sentence	denotes	But when given liver X receptor alpha (LXRα) agonist, Abcg5/sterolin-1 and Abcg8/sterolin-2 appeared to be expressed at the apical surface.
T14258	48444-48447	CC	denotes	But
T14259	48536-48544	VBD	denotes	appeared
T14260	48448-48452	WRB	denotes	when
T14261	48453-48458	VBN	denotes	given
T14262	48459-48464	NN	denotes	liver
T14263	48476-48481	NN	denotes	alpha
T14264	48465-48466	NN	denotes	X
T14265	48467-48475	NN	denotes	receptor
T14266	48489-48496	NN	denotes	agonist
T14267	48482-48483	-LRB-	denotes	(
T14268	48483-48487	NN	denotes	LXRα
T14269	48487-48488	-RRB-	denotes	)
T14270	48496-48498	,	denotes	,
T14271	48498-48503	NN	denotes	Abcg5
T14272	48504-48512	NN	denotes	sterolin
T14273	48503-48504	HYPH	denotes	/
T14274	48512-48513	HYPH	denotes	-
T14275	48513-48514	CD	denotes	1
T14276	48515-48518	CC	denotes	and
T14277	48519-48524	NN	denotes	Abcg8
T14278	48525-48533	NN	denotes	sterolin
T14279	48524-48525	HYPH	denotes	/
T14280	48533-48534	HYPH	denotes	-
T14281	48534-48535	CD	denotes	2
T14282	48545-48547	TO	denotes	to
T14283	48551-48560	VBN	denotes	expressed
T14284	48548-48550	VB	denotes	be
T14285	48561-48563	IN	denotes	at
T14286	48564-48567	DT	denotes	the
T14287	48575-48582	NN	denotes	surface
T14288	48568-48574	JJ	denotes	apical
T14289	48582-48583	.	denotes	.
T14290	48583-48650	sentence	denotes	In these studies the UTSW anti-Abcg5/sterolin-1 antibody was used.
T14292	48645-48649	VBN	denotes	used
T14293	48587-48592	DT	denotes	these
T14294	48593-48600	NNS	denotes	studies
T14295	48601-48604	DT	denotes	the
T14296	48632-48640	NN	denotes	antibody
T14297	48605-48609	NN	denotes	UTSW
T14298	48610-48620	NN	denotes	anti-Abcg5
T14299	48621-48629	NN	denotes	sterolin
T14300	48620-48621	HYPH	denotes	/
T14301	48629-48630	HYPH	denotes	-
T14302	48630-48631	CD	denotes	1
T14303	48641-48644	VBD	denotes	was
T14304	48649-48650	.	denotes	.
T14305	48650-48757	sentence	denotes	It is apparent with these conflicting data that the trafficking of these transporters is not as clear-cut.
T14306	48651-48653	PRP	denotes	It
T14307	48654-48656	VBZ	denotes	is
T14308	48657-48665	JJ	denotes	apparent
T14309	48666-48670	IN	denotes	with
T14310	48671-48676	DT	denotes	these
T14311	48689-48693	NNS	denotes	data
T14312	48677-48688	VBG	denotes	conflicting
T14313	48694-48698	IN	denotes	that
T14314	48737-48739	VBZ	denotes	is
T14315	48699-48702	DT	denotes	the
T14316	48703-48714	NN	denotes	trafficking
T14317	48715-48717	IN	denotes	of
T14318	48718-48723	DT	denotes	these
T14319	48724-48736	NNS	denotes	transporters
T14320	48740-48743	RB	denotes	not
T14321	48744-48746	RB	denotes	as
T14322	48753-48756	JJ	denotes	cut
T14323	48747-48752	JJ	denotes	clear
T14324	48752-48753	HYPH	denotes	-
T14325	48756-48757	.	denotes	.
T14326	48757-48914	sentence	denotes	In a recent paper, Mangrivite et al. showed that the apical sorting of rat SPNT in polarized renal epithelial cells was independent of N-glycosylation [45].
T14327	48758-48760	IN	denotes	In
T14328	48795-48801	VBD	denotes	showed
T14329	48761-48762	DT	denotes	a
T14330	48770-48775	NN	denotes	paper
T14331	48763-48769	JJ	denotes	recent
T14332	48775-48777	,	denotes	,
T14333	48777-48787	NNP	denotes	Mangrivite
T14334	48788-48790	FW	denotes	et
T14335	48791-48794	FW	denotes	al.
T14336	48802-48806	IN	denotes	that
T14337	48874-48877	VBD	denotes	was
T14338	48807-48810	DT	denotes	the
T14339	48818-48825	NN	denotes	sorting
T14340	48811-48817	JJ	denotes	apical
T14341	48826-48828	IN	denotes	of
T14342	48829-48832	NN	denotes	rat
T14343	48833-48837	NN	denotes	SPNT
T14344	48838-48840	IN	denotes	in
T14345	48841-48850	VBN	denotes	polarized
T14346	48868-48873	NNS	denotes	cells
T14347	48851-48856	JJ	denotes	renal
T14348	48857-48867	JJ	denotes	epithelial
T14349	48878-48889	JJ	denotes	independent
T14350	48890-48892	IN	denotes	of
T14351	48893-48894	NN	denotes	N
T14352	48895-48908	NN	denotes	glycosylation
T14353	48894-48895	HYPH	denotes	-
T14354	48909-48910	-LRB-	denotes	[
T14355	48910-48912	CD	denotes	45
T14356	48912-48913	-RRB-	denotes	]
T14357	48913-48914	.	denotes	.
T14358	48914-49088	sentence	denotes	Therefore, at this time, we can neither exclude the possibility that Abcg5/sterolin-1 expression is functional in our Abcg8/sterolin-2 deficient mice nor can we rule it out.
T14359	48915-48924	RB	denotes	Therefore
T14360	48955-48962	VB	denotes	exclude
T14361	48924-48926	,	denotes	,
T14362	48926-48928	IN	denotes	at
T14363	48929-48933	DT	denotes	this
T14364	48934-48938	NN	denotes	time
T14365	48938-48940	,	denotes	,
T14366	48940-48942	PRP	denotes	we
T14367	48943-48946	MD	denotes	can
T14368	48947-48954	RB	denotes	neither
T14369	48963-48966	DT	denotes	the
T14370	48967-48978	NN	denotes	possibility
T14371	48979-48983	IN	denotes	that
T14372	49012-49014	VBZ	denotes	is
T14373	48984-48989	NN	denotes	Abcg5
T14374	48990-48998	NN	denotes	sterolin
T14375	48989-48990	HYPH	denotes	/
T14376	49001-49011	NN	denotes	expression
T14377	48998-48999	HYPH	denotes	-
T14378	48999-49000	CD	denotes	1
T14379	49015-49025	JJ	denotes	functional
T14380	49026-49028	IN	denotes	in
T14381	49029-49032	PRP$	denotes	our
T14382	49060-49064	NNS	denotes	mice
T14383	49033-49038	NN	denotes	Abcg8
T14384	49039-49047	NN	denotes	sterolin
T14385	49038-49039	HYPH	denotes	/
T14386	49050-49059	JJ	denotes	deficient
T14387	49047-49048	HYPH	denotes	-
T14388	49048-49049	CD	denotes	2
T14389	49065-49068	CC	denotes	nor
T14390	49069-49072	MD	denotes	can
T14391	49076-49080	VB	denotes	rule
T14392	49073-49075	PRP	denotes	we
T14393	49081-49083	PRP	denotes	it
T14394	49084-49087	RP	denotes	out
T14395	49087-49088	.	denotes	.
T14396	49088-49147	sentence	denotes	We have no functional assay for these proteins at present.
T14397	49089-49091	PRP	denotes	We
T14398	49092-49096	VBP	denotes	have
T14399	49097-49099	DT	denotes	no
T14400	49111-49116	NN	denotes	assay
T14401	49100-49110	JJ	denotes	functional
T14402	49117-49120	IN	denotes	for
T14403	49121-49126	DT	denotes	these
T14404	49127-49135	NN	denotes	proteins
T14405	49136-49138	IN	denotes	at
T14406	49139-49146	JJ	denotes	present
T14407	49146-49147	.	denotes	.
T14408	49147-49296	sentence	denotes	More extensive fractionation experiments are underway to better determine the exact compartmental location of Abcg5/sterolin-1 in the Abcg8-/- mice.
T14409	49148-49152	RBR	denotes	More
T14410	49153-49162	JJ	denotes	extensive
T14411	49177-49188	NNS	denotes	experiments
T14412	49163-49176	NN	denotes	fractionation
T14413	49189-49192	VBP	denotes	are
T14414	49193-49201	JJ	denotes	underway
T14415	49202-49204	TO	denotes	to
T14416	49212-49221	VB	denotes	determine
T14417	49205-49211	RBR	denotes	better
T14418	49222-49225	DT	denotes	the
T14419	49246-49254	NN	denotes	location
T14420	49226-49231	JJ	denotes	exact
T14421	49232-49245	JJ	denotes	compartmental
T14422	49255-49257	IN	denotes	of
T14423	49258-49263	NN	denotes	Abcg5
T14424	49264-49272	NN	denotes	sterolin
T14425	49263-49264	HYPH	denotes	/
T14426	49272-49273	HYPH	denotes	-
T14427	49273-49274	CD	denotes	1
T14428	49275-49277	IN	denotes	in
T14429	49278-49281	DT	denotes	the
T14430	49291-49295	NNS	denotes	mice
T14431	49282-49287	NN	denotes	Abcg8
T14432	49287-49288	SYM	denotes	-
T14433	49288-49289	HYPH	denotes	/
T14434	49289-49290	SYM	denotes	-
T14435	49295-49296	.	denotes	.
T14436	49296-49612	sentence	denotes	In this context, Plösch et al. have described an Abcg5/sterolin-1 deficient mouse that maintains the ability to secrete biliary cholesterol to the same extent as the wild-type mice and, when fed a LXR agonist, had higher Abcg8 mRNA expression and tended to secrete more biliary cholesterol than wild-type mice [46].
T14437	49297-49299	IN	denotes	In
T14438	49333-49342	VBN	denotes	described
T14439	49300-49304	DT	denotes	this
T14440	49305-49312	NN	denotes	context
T14441	49312-49314	,	denotes	,
T14442	49314-49320	NNP	denotes	Plösch
T14443	49321-49323	FW	denotes	et
T14444	49324-49327	FW	denotes	al.
T14445	49328-49332	VBP	denotes	have
T14446	49343-49345	DT	denotes	an
T14447	49373-49378	NN	denotes	mouse
T14448	49346-49351	NN	denotes	Abcg5
T14449	49352-49360	NN	denotes	sterolin
T14450	49351-49352	HYPH	denotes	/
T14451	49363-49372	JJ	denotes	deficient
T14452	49360-49361	HYPH	denotes	-
T14453	49361-49362	CD	denotes	1
T14454	49379-49383	WDT	denotes	that
T14455	49384-49393	VBZ	denotes	maintains
T14456	49394-49397	DT	denotes	the
T14457	49398-49405	NN	denotes	ability
T14458	49406-49408	TO	denotes	to
T14459	49409-49416	VB	denotes	secrete
T14460	49417-49424	JJ	denotes	biliary
T14461	49425-49436	NN	denotes	cholesterol
T14462	49437-49439	IN	denotes	to
T14463	49440-49443	DT	denotes	the
T14464	49449-49455	NN	denotes	extent
T14465	49444-49448	JJ	denotes	same
T14466	49456-49458	IN	denotes	as
T14467	49459-49462	DT	denotes	the
T14468	49473-49477	NNS	denotes	mice
T14469	49463-49467	JJ	denotes	wild
T14470	49468-49472	NN	denotes	type
T14471	49467-49468	HYPH	denotes	-
T14472	49478-49481	CC	denotes	and
T14473	49481-49483	,	denotes	,
T14474	49483-49487	WRB	denotes	when
T14475	49488-49491	VBN	denotes	fed
T14476	49507-49510	VBD	denotes	had
T14477	49492-49493	DT	denotes	a
T14478	49498-49505	NN	denotes	agonist
T14479	49494-49497	NN	denotes	LXR
T14480	49505-49507	,	denotes	,
T14481	49511-49517	JJR	denotes	higher
T14482	49529-49539	NN	denotes	expression
T14483	49518-49523	NN	denotes	Abcg8
T14484	49524-49528	NN	denotes	mRNA
T14485	49540-49543	CC	denotes	and
T14486	49544-49550	VBD	denotes	tended
T14487	49551-49553	TO	denotes	to
T14488	49554-49561	VB	denotes	secrete
T14489	49562-49566	JJR	denotes	more
T14490	49575-49586	NN	denotes	cholesterol
T14491	49567-49574	JJ	denotes	biliary
T14492	49587-49591	IN	denotes	than
T14493	49592-49596	JJ	denotes	wild
T14494	49597-49601	NN	denotes	type
T14495	49596-49597	HYPH	denotes	-
T14496	49602-49606	NNS	denotes	mice
T14497	49607-49608	-LRB-	denotes	[
T14498	49608-49610	CD	denotes	46
T14499	49610-49611	-RRB-	denotes	]
T14500	49611-49612	.	denotes	.
T14501	49612-49753	sentence	denotes	It has been argued that Abcg5/sterolin-1 and Abcg8/sterolin-2 are dependent upon each other for secretion of hepatic sterols into bile [19].
T14502	49613-49615	PRP	denotes	It
T14503	49625-49631	VBN	denotes	argued
T14504	49616-49619	VBZ	denotes	has
T14505	49620-49624	VBN	denotes	been
T14506	49632-49636	IN	denotes	that
T14507	49675-49678	VBP	denotes	are
T14508	49637-49642	NN	denotes	Abcg5
T14509	49643-49651	NN	denotes	sterolin
T14510	49642-49643	HYPH	denotes	/
T14511	49651-49652	HYPH	denotes	-
T14512	49652-49653	CD	denotes	1
T14513	49654-49657	CC	denotes	and
T14514	49658-49663	NN	denotes	Abcg8
T14515	49664-49672	NN	denotes	sterolin
T14516	49663-49664	HYPH	denotes	/
T14517	49672-49673	HYPH	denotes	-
T14518	49673-49674	CD	denotes	2
T14519	49679-49688	JJ	denotes	dependent
T14520	49689-49693	IN	denotes	upon
T14521	49694-49698	DT	denotes	each
T14522	49699-49704	JJ	denotes	other
T14523	49705-49708	IN	denotes	for
T14524	49709-49718	NN	denotes	secretion
T14525	49719-49721	IN	denotes	of
T14526	49722-49729	JJ	denotes	hepatic
T14527	49730-49737	NNS	denotes	sterols
T14528	49738-49742	IN	denotes	into
T14529	49743-49747	NN	denotes	bile
T14530	49748-49749	-LRB-	denotes	[
T14531	49749-49751	CD	denotes	19
T14532	49751-49752	-RRB-	denotes	]
T14533	49752-49753	.	denotes	.
T14534	49753-49855	sentence	denotes	Given the data presented here and from the Abcg5-deficient mouse this does not appear to be the case.
T14535	49754-49759	VBN	denotes	Given
T14536	49833-49839	VB	denotes	appear
T14537	49760-49763	DT	denotes	the
T14538	49764-49768	NNS	denotes	data
T14539	49769-49778	VBN	denotes	presented
T14540	49779-49783	RB	denotes	here
T14541	49784-49787	CC	denotes	and
T14542	49788-49792	IN	denotes	from
T14543	49793-49796	DT	denotes	the
T14544	49813-49818	NN	denotes	mouse
T14545	49797-49802	NN	denotes	Abcg5
T14546	49803-49812	JJ	denotes	deficient
T14547	49802-49803	HYPH	denotes	-
T14548	49819-49823	DT	denotes	this
T14549	49824-49828	VBZ	denotes	does
T14550	49829-49832	RB	denotes	not
T14551	49840-49842	TO	denotes	to
T14552	49843-49845	VB	denotes	be
T14553	49846-49849	DT	denotes	the
T14554	49850-49854	NN	denotes	case
T14555	49854-49855	.	denotes	.
T14556	49855-49962	sentence	denotes	Perhaps in the previously published model a non-physiologic state has been made that generates these data.
T14557	49856-49863	RB	denotes	Perhaps
T14558	49931-49935	VBN	denotes	made
T14559	49864-49866	IN	denotes	in
T14560	49867-49870	DT	denotes	the
T14561	49892-49897	NN	denotes	model
T14562	49871-49881	RB	denotes	previously
T14563	49882-49891	VBN	denotes	published
T14564	49898-49899	DT	denotes	a
T14565	49916-49921	NN	denotes	state
T14566	49900-49915	JJ	denotes	non-physiologic
T14567	49922-49925	VBZ	denotes	has
T14568	49926-49930	VBN	denotes	been
T14569	49936-49940	WDT	denotes	that
T14570	49941-49950	VBZ	denotes	generates
T14571	49951-49956	DT	denotes	these
T14572	49957-49961	NNS	denotes	data
T14573	49961-49962	.	denotes	.
T14574	49962-50169	sentence	denotes	Taken together, these animal models suggest that Abcg5/sterolin-1 and Abcg8/sterolin-2 have independent function in vivo or that there are proteins other than the sterolins that can secrete biliary sterols.
T14575	49963-49968	VBN	denotes	Taken
T14576	49999-50006	VBP	denotes	suggest
T14577	49969-49977	RB	denotes	together
T14578	49977-49979	,	denotes	,
T14579	49979-49984	DT	denotes	these
T14580	49992-49998	NNS	denotes	models
T14581	49985-49991	NN	denotes	animal
T14582	50007-50011	IN	denotes	that
T14583	50050-50054	VBP	denotes	have
T14584	50012-50017	NN	denotes	Abcg5
T14585	50018-50026	NN	denotes	sterolin
T14586	50017-50018	HYPH	denotes	/
T14587	50026-50027	HYPH	denotes	-
T14588	50027-50028	CD	denotes	1
T14589	50029-50032	CC	denotes	and
T14590	50033-50038	NN	denotes	Abcg8
T14591	50039-50047	NN	denotes	sterolin
T14592	50038-50039	HYPH	denotes	/
T14593	50047-50048	HYPH	denotes	-
T14594	50048-50049	CD	denotes	2
T14595	50055-50066	JJ	denotes	independent
T14596	50067-50075	NN	denotes	function
T14597	50076-50078	FW	denotes	in
T14598	50079-50083	FW	denotes	vivo
T14599	50084-50086	CC	denotes	or
T14600	50087-50091	IN	denotes	that
T14601	50098-50101	VBP	denotes	are
T14602	50092-50097	EX	denotes	there
T14603	50102-50110	NN	denotes	proteins
T14604	50111-50116	JJ	denotes	other
T14605	50117-50121	IN	denotes	than
T14606	50122-50125	DT	denotes	the
T14607	50126-50135	NNS	denotes	sterolins
T14680	50183-50186	DT	denotes	The
T14681	50193-50201	NNS	denotes	findings
T14682	50187-50192	JJ	denotes	major
T14683	50216-50219	VBP	denotes	are
T14684	50202-50204	IN	denotes	in
T14685	50205-50209	DT	denotes	this
T14686	50210-50215	NN	denotes	study
T14687	50219-50221	:	denotes	:
T14688	50221-50222	-LRB-	denotes	(
T14689	50222-50223	LS	denotes	1
T14690	50291-50293	VBZ	denotes	is
T14691	50223-50224	-RRB-	denotes	)
T14692	50225-50235	NN	denotes	disruption
T14693	50236-50238	IN	denotes	of
T14694	50239-50243	RB	denotes	only
T14695	50244-50247	CD	denotes	one
T14696	50248-50250	IN	denotes	of
T14697	50251-50254	DT	denotes	the
T14698	50259-50264	NNS	denotes	genes
T14699	50255-50258	CD	denotes	two
T14700	50265-50275	VBG	denotes	comprising
T14701	50276-50279	DT	denotes	the
T14702	50285-50290	NN	denotes	locus
T14703	50280-50284	NN	denotes	STSL
T14704	50294-50303	JJ	denotes	necessary
T14705	50304-50307	CC	denotes	and
T14706	50308-50318	JJ	denotes	sufficient
T14707	50319-50321	TO	denotes	to
T14708	50322-50327	VB	denotes	cause
T14709	50328-50342	NN	denotes	sitosterolemia
T14710	50342-50344	,	denotes	,
T14711	50344-50345	-LRB-	denotes	(
T14712	50345-50346	LS	denotes	2
T14713	50382-50387	VBZ	denotes	leads
T14714	50346-50347	-RRB-	denotes	)
T14715	50348-50352	IN	denotes	that
T14716	50353-50356	DT	denotes	the
T14717	50357-50361	NN	denotes	loss
T14718	50362-50364	IN	denotes	of
T14719	50365-50370	NN	denotes	Abcg8
T14720	50371-50379	NN	denotes	sterolin
T14721	50370-50371	HYPH	denotes	/
T14722	50379-50380	HYPH	denotes	-
T14723	50380-50381	CD	denotes	2
T14724	50388-50390	IN	denotes	to
T14725	50391-50394	DT	denotes	the
T14726	50395-50399	NN	denotes	loss
T14727	50400-50402	IN	denotes	of
T14728	50403-50410	JJ	denotes	biliary
T14729	50423-50432	NN	denotes	secretion
T14730	50411-50422	NN	denotes	cholesterol
T14731	50433-50436	CC	denotes	and
T14732	50437-50438	-LRB-	denotes	(
T14733	50438-50439	LS	denotes	3
T14734	50458-50465	VBZ	denotes	appears
T14735	50439-50440	-RRB-	denotes	)
T14736	50441-50446	NN	denotes	Abcg5
T14737	50447-50455	NN	denotes	sterolin
T14738	50446-50447	HYPH	denotes	/
T14739	50455-50456	HYPH	denotes	-
T14740	50456-50457	CD	denotes	1
T14741	50466-50468	TO	denotes	to
T14742	50487-50496	VBN	denotes	expressed
T14743	50469-50471	VB	denotes	be
T14744	50472-50477	RB	denotes	still
T14745	50478-50486	RB	denotes	apically
T14746	50486-50487	HYPH	denotes	-
T14747	50497-50504	IN	denotes	despite
T14748	50505-50508	DT	denotes	the
T14749	50509-50516	NN	denotes	absence
T14750	50517-50519	IN	denotes	of
T14751	50520-50525	NN	denotes	Abcg8
T14752	50526-50534	NN	denotes	sterolin
T14753	50525-50526	HYPH	denotes	/
T14754	50534-50535	HYPH	denotes	-
T14755	50535-50536	CD	denotes	2
T14756	50536-50537	.	denotes	.
T14757	50537-50715	sentence	denotes	These data strongly support the direct role of Abcg8/sterolin-2 as a key player in biliary cholesterol secretion, although biliary sitosterol secretion was apparently preserved.
T14758	50538-50543	DT	denotes	These
T14759	50544-50548	NNS	denotes	data
T14760	50558-50565	VBP	denotes	support
T14761	50549-50557	RB	denotes	strongly
T14762	50566-50569	DT	denotes	the
T14763	50577-50581	NN	denotes	role
T14764	50570-50576	JJ	denotes	direct
T14765	50582-50584	IN	denotes	of
T14766	50585-50590	NN	denotes	Abcg8
T14767	50591-50599	NN	denotes	sterolin
T14768	50590-50591	HYPH	denotes	/
T14769	50599-50600	HYPH	denotes	-
T14770	50600-50601	CD	denotes	2
T14771	50602-50604	IN	denotes	as
T14772	50605-50606	DT	denotes	a
T14773	50611-50617	NN	denotes	player
T14774	50607-50610	JJ	denotes	key
T14775	50618-50620	IN	denotes	in
T14776	50621-50628	JJ	denotes	biliary
T14777	50629-50640	NN	denotes	cholesterol
T14778	50641-50650	NN	denotes	secretion
T14779	50650-50652	,	denotes	,
T14780	50652-50660	IN	denotes	although
T14781	50705-50714	VBN	denotes	preserved
T14782	50661-50668	JJ	denotes	biliary
T14783	50669-50679	NN	denotes	sitosterol
T14784	50680-50689	NN	denotes	secretion
T14785	50690-50693	VBD	denotes	was
T14786	50694-50704	RB	denotes	apparently
T14787	50714-50715	.	denotes	.
T14788	50715-50842	sentence	denotes	These data suggest that there are mechanisms other than Abcg8/sterolin-2 that allow for the secretion of sitosterol into bile.
T14789	50716-50721	DT	denotes	These
T14790	50722-50726	NNS	denotes	data
T14791	50727-50734	VBP	denotes	suggest
T14792	50735-50739	IN	denotes	that
T14793	50746-50749	VBP	denotes	are
T14794	50740-50745	EX	denotes	there
T14795	50750-50760	NNS	denotes	mechanisms
T14796	50761-50766	JJ	denotes	other
T14797	50767-50771	IN	denotes	than
T14798	50772-50777	NN	denotes	Abcg8
T14799	50778-50786	NN	denotes	sterolin
T14800	50777-50778	HYPH	denotes	/
T14801	50786-50787	HYPH	denotes	-
T14802	50787-50788	CD	denotes	2
T14803	50789-50793	WDT	denotes	that
T14804	50794-50799	VBP	denotes	allow
T14805	50800-50803	IN	denotes	for
T14806	50804-50807	DT	denotes	the
T14807	50808-50817	NN	denotes	secretion
T14808	50818-50820	IN	denotes	of
T14809	50821-50831	NN	denotes	sitosterol
T14810	50832-50836	IN	denotes	into
T14811	50837-50841	NN	denotes	bile
T14812	50841-50842	.	denotes	.
T14813	51665-51668	DT	denotes	The
T14814	51685-51692	NN	denotes	history
T14815	51669-51684	JJ	denotes	pre-publication
T14816	51715-51723	VBN	denotes	accessed
T14817	51693-51696	IN	denotes	for
T14818	51697-51701	DT	denotes	this
T14819	51702-51707	NN	denotes	paper
T14820	51708-51711	MD	denotes	can
T14821	51712-51714	VB	denotes	be
T14822	51724-51728	RB	denotes	here
T14823	51728-51729	:	denotes	:
T4782	17141-17151	JJ	denotes	Polyclonal
T4783	17161-17171	NN	denotes	production
T4784	17152-17160	NN	denotes	antibody
T4785	17171-17283	sentence	denotes	Three separate polyclonal anti-Abcg5/sterolin-1 antibodies have been raised as previously described [20,29,30].
T4786	17172-17177	CD	denotes	Three
T4787	17220-17230	NNS	denotes	antibodies
T4788	17178-17186	JJ	denotes	separate
T4789	17187-17197	JJ	denotes	polyclonal
T4790	17198-17208	JJ	denotes	anti-Abcg5
T4791	17209-17217	NN	denotes	sterolin
T4792	17208-17209	HYPH	denotes	/
T4793	17217-17218	HYPH	denotes	-
T4794	17218-17219	CD	denotes	1
T4795	17241-17247	VBN	denotes	raised
T4796	17231-17235	VBP	denotes	have
T4797	17236-17240	VBN	denotes	been
T4798	17248-17250	IN	denotes	as
T4799	17262-17271	VBN	denotes	described
T4800	17251-17261	RB	denotes	previously
T257	0-1	DT	denotes	A
T4153	14980-14983	VBD	denotes	was
T9953	33463-33467	VBD	denotes	were
T14291	48584-48586	IN	denotes	In
T14608	50136-50140	WDT	denotes	that
T14609	50145-50152	VB	denotes	secrete
T14610	50141-50144	MD	denotes	can
T14611	50153-50160	JJ	denotes	biliary
T14612	50161-50168	NNS	denotes	sterols
T14613	50168-50169	.	denotes	.
R4974	T7483	T7484	amod	normal,splicing
R4975	T7484	T7482	pobj	splicing,of
R4976	T7485	T7480	punct	", ",disruption
R4977	T7486	T7487	advmod	as,as
R4978	T7487	T7480	cc	as,disruption
R4979	T7488	T7487	advmod	well,as
R4980	T7489	T7480	conj	loss,disruption
R4981	T7490	T7489	prep	of,loss
R4982	T7491	T7492	det	some,sequences
R4983	T7492	T7490	pobj	sequences,of
R4984	T7493	T7492	amod	coding,sequences
R4985	T7494	T7492	acl	involving,sequences
R4986	T7495	T7496	nmod	exons,3
R4987	T7496	T7494	dobj	3,involving
R4988	T7497	T7496	cc	and,3
R4989	T7498	T7496	conj	4,3
R4990	T7499	T7473	punct	.,results
R4991	T7501	T7502	prep	After,identified
R4992	T7503	T7504	compound	ES,cell
R4993	T7504	T7505	compound	cell,electroporation
R4994	T7505	T7501	pobj	electroporation,After
R4995	T7506	T7505	prep	with,electroporation
R4996	T7507	T7508	det	the,DNA
R4997	T7508	T7506	pobj	DNA,with
R4998	T7509	T7508	amod	linearized,DNA
R4999	T7510	T7508	amod	targeted,DNA
R5000	T7511	T7508	compound	plasmid,DNA
R5001	T7512	T7502	punct	", ",identified
R5002	T7513	T7514	nummod	two,clones
R5003	T7514	T7502	nsubjpass	clones,identified
R5004	T7515	T7514	amod	homologous,clones
R5005	T7516	T7514	amod	recombinant,clones
R5006	T7517	T7514	compound	ES,clones
R5007	T7518	T7514	punct	(,clones
R5008	T7519	T7514	prep	out,clones
R5009	T7520	T7519	prep	of,out
R5010	T7521	T7522	nummod	58,clones
R5011	T7522	T7520	pobj	clones,of
R5012	T7523	T7522	compound	ES,clones
R5013	T7524	T7522	acl	screened,clones
R5014	T7525	T7502	punct	),identified
R5015	T7526	T7502	auxpass	were,identified
R5016	T7527	T7502	punct	.,identified
R5017	T7529	T7530	compound	Blastocyst,injection
R5018	T7530	T7531	nsubj	injection,resulted
R5019	T7532	T7530	prep	of,injection
R5020	T7533	T7532	pobj	these,of
R5021	T7534	T7531	prep	in,resulted
R5022	T7535	T7536	nummod	five,mice
R5023	T7536	T7534	pobj	mice,in
R5024	T7537	T7538	advmod	highly,chimeric
R5025	T7538	T7536	amod	chimeric,mice
R5026	T7539	T7540	punct	(,males
R5027	T7540	T7536	parataxis	males,mice
R5028	T7541	T7540	nummod	three,males
R5029	T7542	T7540	punct	", ",males
R5030	T7543	T7544	nummod	two,females
R5031	T7544	T7540	appos	females,males
R5032	T7545	T7540	punct	),males
R5033	T7546	T7531	punct	.,resulted
R5034	T7548	T7549	nummod	One,male
R5035	T7549	T7550	nsubj	male,showed
R5036	T7551	T7549	cc	and,male
R5037	T7552	T7553	nummod	one,female
R5038	T7553	T7549	conj	female,male
R5039	T7554	T7549	punct	", ",male
R5040	T7555	T7556	dep	both,from
R5041	T7556	T7549	relcl	from,male
R5042	T7557	T7558	det	the,clone
R5043	T7558	T7556	pobj	clone,from
R5044	T7559	T7558	amod	same,clone
R5045	T7560	T7558	compound	ES,clone
R5046	T7561	T7550	punct	", ",showed
R5047	T7562	T7550	dep	germline,showed
R5048	T7563	T7550	dep	transmission,showed
R5049	T7564	T7550	cc	and,showed
R5050	T7565	T7566	det	the,line
R5051	T7566	T7568	nsubjpass	line,used
R5052	T7567	T7566	amod	female,line
R5053	T7568	T7550	conj	used,showed
R5054	T7569	T7568	auxpass	was,used
R5055	T7570	T7571	aux	to,establish
R5056	T7571	T7568	advcl	establish,used
R5057	T7572	T7573	det	a,colony
R5058	T7573	T7571	dobj	colony,establish
R5059	T7574	T7573	amod	multi-generational,colony
R5060	T7575	T7573	acl	bred,colony
R5061	T7576	T7575	prep	onto,bred
R5062	T7577	T7578	det	a,background
R5063	T7578	T7576	pobj	background,onto
R5064	T7579	T7580	compound	C57Bl,6J
R5065	T7580	T7578	compound	6J,background
R5066	T7581	T7580	punct	/,6J
R5067	T7582	T7568	punct	.,used
R5068	T7584	T7585	amod	Heterozygous,mice
R5069	T7585	T7586	nsubj	mice,were
R5070	T7587	T7586	acomp	fertile,were
R5071	T7588	T7586	cc	and,were
R5072	T7589	T7586	conj	gave,were
R5073	T7590	T7589	dobj	rise,gave
R5074	T7591	T7589	prep	to,gave
R5075	T7592	T7593	amod	normal,sizes
R5076	T7593	T7591	pobj	sizes,to
R5077	T7594	T7593	amod	litter,sizes
R5078	T7595	T7586	punct	.,were
R5079	T7597	T7598	csubj	Breeding,led
R5080	T7599	T7600	amod	heterozygous,animals
R5081	T7600	T7597	dobj	animals,Breeding
R5082	T7601	T7598	prep	to,led
R5083	T7602	T7603	amod	wild,type
R5084	T7603	T7605	nmod	type,mice
R5085	T7604	T7603	punct	-,type
R5086	T7605	T7601	pobj	mice,to
R5087	T7606	T7603	punct	", ",type
R5088	T7607	T7608	amod	heterozygous,knockout
R5089	T7608	T7603	appos	knockout,type
R5090	T7609	T7607	cc	and,heterozygous
R5091	T7610	T7607	conj	homozygous,heterozygous
R5092	T7611	T7605	prep	in,mice
R5093	T7612	T7613	det	the,distribution
R5094	T7613	T7611	pobj	distribution,in
R5095	T7614	T7613	amod	expected,distribution
R5096	T7615	T7613	amod	Mendelian,distribution
R5097	T7616	T7617	punct	(,shown
R5098	T7617	T7598	parataxis	shown,led
R5099	T7618	T7617	nsubj	data,shown
R5100	T7619	T7617	neg	not,shown
R5101	T7620	T7617	punct	),shown
R5102	T7621	T7598	punct	.,led
R5103	T7623	T7624	mark	Although,quantitative
R5104	T7624	T7626	advcl	quantitative,showed
R5105	T7625	T7624	neg	not,quantitative
R5106	T7627	T7626	punct	", ",showed
R5107	T7628	T7629	compound	Northern,analyses
R5108	T7629	T7626	nsubj	analyses,showed
R5109	T7630	T7631	mark	that,appeared
R5110	T7631	T7626	ccomp	appeared,showed
R5111	T7632	T7633	mark	while,showed
R5112	T7633	T7631	advcl	showed,appeared
R5113	T7634	T7635	det	the,mice
R5114	T7635	T7633	nsubj	mice,showed
R5115	T7636	T7635	compound	knockout,mice
R5116	T7637	T7638	det	no,mRNA
R5117	T7638	T7633	dobj	mRNA,showed
R5118	T7639	T7638	amod	detectable,mRNA
R5119	T7640	T7638	prep	for,mRNA
R5120	T7641	T7640	pobj	Abcg8,for
R5121	T7642	T7631	punct	", ",appeared
R5122	T7643	T7631	nsubj	expression,appeared
R5123	T7644	T7643	prep	of,expression
R5124	T7645	T7644	pobj	Abcg5,of
R5125	T7646	T7631	oprd	unaltered,appeared
R5126	T7647	T7648	punct	(,1c
R5127	T7648	T7626	parataxis	1c,showed
R5128	T7649	T7648	compound	Figure,1c
R5129	T7650	T7648	punct	),1c
R5130	T7651	T7626	punct	.,showed
R5131	T7653	T7654	aux	To,exclude
R5132	T7654	T7655	advcl	exclude,performed
R5133	T7656	T7657	det	the,possibility
R5134	T7657	T7654	dobj	possibility,exclude
R5135	T7658	T7657	prep	of,possibility
R5136	T7659	T7660	amod	alternative,splicing
R5137	T7660	T7658	pobj	splicing,of
R5138	T7661	T7660	prep	with,splicing
R5139	T7662	T7663	det	the,production
R5140	T7663	T7661	pobj	production,with
R5141	T7664	T7663	prep	of,production
R5142	T7665	T7666	det	a,protein
R5143	T7666	T7664	pobj	protein,of
R5144	T7667	T7666	amod	non-functional,protein
R5145	T7668	T7667	cc	but,non-functional
R5146	T7669	T7667	conj	truncated,non-functional
R5147	T7670	T7671	nmod	Abcg8,sterolin
R5148	T7671	T7666	nmod	sterolin,protein
R5149	T7672	T7671	punct	/,sterolin
R5150	T7673	T7671	punct	-,sterolin
R5151	T7674	T7671	nummod	2,sterolin
R5152	T7675	T7676	dep	that,serve
R5153	T7676	T7666	relcl	serve,protein
R5154	T7677	T7676	aux	may,serve
R5155	T7678	T7676	prep	as,serve
R5156	T7679	T7680	det	a,chaperone
R5157	T7680	T7678	pobj	chaperone,as
R5158	T7681	T7680	prep	for,chaperone
R5159	T7682	T7683	compound	Abcg5,sterolin
R5160	T7683	T7681	pobj	sterolin,for
R5161	T7684	T7683	punct	/,sterolin
R5162	T7685	T7683	punct	-,sterolin
R5163	T7686	T7683	nummod	1,sterolin
R5164	T7687	T7655	punct	", ",performed
R5165	T7688	T7689	compound	RT,PCR
R5166	T7689	T7655	nsubjpass	PCR,performed
R5167	T7690	T7689	punct	-,PCR
R5168	T7691	T7655	auxpass	was,performed
R5169	T7692	T7655	prep	on,performed
R5170	T7693	T7692	pobj	cDNA,on
R5171	T7694	T7695	advmod	reverse,transcribed
R5172	T7695	T7693	acl	transcribed,cDNA
R5173	T7696	T7695	prep	from,transcribed
R5174	T7697	T7698	amod	total,RNA
R5175	T7698	T7696	pobj	RNA,from
R5176	T7699	T7698	compound	liver,RNA
R5177	T7700	T7698	prep	from,RNA
R5178	T7701	T7702	npadvmod	Abcg8,deficient
R5179	T7702	T7704	amod	deficient,mice
R5180	T7703	T7702	punct	-,deficient
R5181	T7704	T7700	pobj	mice,from
R5182	T7705	T7655	punct	.,performed
R5183	T7707	T7708	det	No,mRNA
R5184	T7708	T7709	nsubjpass	mRNA,containing
R5185	T7710	T7708	prep	for,mRNA
R5186	T7711	T7712	compound	Abcg8,sterolin
R5187	T7712	T7710	pobj	sterolin,for
R5188	T7713	T7712	punct	/,sterolin
R5189	T7714	T7712	punct	-,sterolin
R5190	T7715	T7712	nummod	2,sterolin
R5191	T7716	T7709	auxpass	was,containing
R5192	T7717	T7709	aux	detected,containing
R5193	T7718	T7719	det	any,sequences
R5194	T7719	T7709	dobj	sequences,containing
R5195	T7720	T7719	advmod	downstream,sequences
R5196	T7721	T7720	prep	of,downstream
R5197	T7722	T7721	pobj	exon,of
R5198	T7723	T7722	nummod	4,exon
R5199	T7724	T7709	prep	in,containing
R5200	T7725	T7726	det	the,mice
R5201	T7726	T7724	pobj	mice,in
R5202	T7727	T7728	npadvmod	Abcg8,deficient
R5203	T7728	T7726	amod	deficient,mice
R5204	T7729	T7728	punct	-,deficient
R5205	T7730	T7709	punct	", ",containing
R5206	T7731	T7732	mark	whether,located
R5207	T7732	T7709	advcl	located,containing
R5208	T7733	T7732	nsubjpass	primers,located
R5209	T7734	T7732	auxpass	were,located
R5210	T7735	T7732	prep	in,located
R5211	T7736	T7737	nmod	exons,4
R5212	T7737	T7735	pobj	4,in
R5213	T7738	T7737	cc	and,4
R5214	T7739	T7737	conj	13,4
R5215	T7740	T7737	punct	", ",4
R5216	T7741	T7742	nmod	exons,9
R5217	T7742	T7737	conj	9,4
R5218	T7743	T7742	cc	and,9
R5219	T7744	T7742	conj	13,9
R5220	T7745	T7742	cc	or,9
R5221	T7746	T7747	nmod	exons,10
R5222	T7747	T7742	conj	10,9
R5223	T7748	T7747	cc	and,10
R5224	T7749	T7747	conj	13,10
R5225	T7750	T7751	punct	(,1e
R5226	T7751	T7709	parataxis	1e,containing
R5227	T7752	T7751	nmod	Figure,1e
R5228	T7753	T7751	nmod	1d,1e
R5229	T7754	T7751	punct	",",1e
R5230	T7755	T7751	punct	),1e
R5231	T7756	T7709	punct	.,containing
R5232	T7758	T7759	nsubj	This,demonstrates
R5233	T7760	T7761	mark	that,is
R5234	T7761	T7759	ccomp	is,demonstrates
R5235	T7762	T7763	mark	if,code
R5236	T7763	T7761	advcl	code,is
R5237	T7764	T7765	det	an,message
R5238	T7765	T7763	nsubj	message,code
R5239	T7766	T7767	advmod	alternatively,spliced
R5240	T7767	T7765	amod	spliced,message
R5241	T7768	T7763	aux	could,code
R5242	T7769	T7763	advmod	potentially,code
R5243	T7770	T7763	prep	for,code
R5244	T7771	T7772	det	a,protein
R5245	T7772	T7770	pobj	protein,for
R5246	T7773	T7772	amod	truncated,protein
R5247	T7774	T7761	punct	", ",is
R5248	T7775	T7761	nsubj	it,is
R5249	T7776	T7761	neg	not,is
R5250	T7777	T7761	acomp	detectable,is
R5251	T7778	T7761	prep	in,is
R5252	T7779	T7780	det	the,mice
R5253	T7780	T7778	pobj	mice,in
R5254	T7781	T7782	npadvmod	Abcg8,deficient
R5255	T7782	T7780	amod	deficient,mice
R5256	T7783	T7782	punct	-,deficient
R5257	T7784	T7759	punct	.,demonstrates
R5258	T7786	T7787	det	The,domains
R5259	T7787	T7791	nsubjpass	domains,encoded
R5260	T7788	T7789	npadvmod	membrane,spanning
R5261	T7789	T7787	amod	spanning,domains
R5262	T7790	T7789	punct	-,spanning
R5263	T7792	T7787	prep	of,domains
R5264	T7793	T7794	compound	Abcg8,sterolin
R5265	T7794	T7792	pobj	sterolin,of
R5266	T7795	T7794	punct	/,sterolin
R5267	T7796	T7794	punct	-,sterolin
R5268	T7797	T7794	nummod	2,sterolin
R5269	T7798	T7791	auxpass	are,encoded
R5270	T7799	T7791	agent	by,encoded
R5271	T7800	T7801	nmod	exons,9
R5272	T7801	T7799	pobj	9,by
R5273	T7802	T7803	punct	–,13
R5274	T7803	T7801	prep	13,9
R5275	T7804	T7791	punct	.,encoded
R5284	T8132	T8133	compound	Sterol,levels
R5285	T8134	T8133	prep	in,levels
R5286	T8135	T8136	compound	Abcg8,sterolin
R5287	T8136	T8138	npadvmod	sterolin,deficient
R5288	T8137	T8136	punct	/,sterolin
R5289	T8138	T8141	amod	deficient,animals
R5290	T8139	T8136	punct	-,sterolin
R5291	T8140	T8136	nummod	2,sterolin
R5292	T8141	T8134	pobj	animals,in
R5293	T8143	T8144	amod	Elevated,sterols
R5294	T8144	T8146	nsubj	sterols,are
R5295	T8145	T8144	compound	plant,sterols
R5296	T8147	T8144	prep	in,sterols
R5297	T8148	T8147	pobj	tissue,in
R5298	T8149	T8148	cc	and,tissue
R5299	T8150	T8148	conj	plasma,tissue
R5300	T8151	T8146	acomp	diagnostic,are
R5301	T8152	T8151	prep	of,diagnostic
R5302	T8153	T8152	pobj	sitosterolemia,of
R5303	T8154	T8146	punct	.,are
R5304	T8156	T8157	compound	Sterol,levels
R5305	T8157	T8158	nsubjpass	levels,shown
R5306	T8159	T8157	punct	", ",levels
R5307	T8160	T8161	mark	as,determined
R5308	T8161	T8157	advcl	determined,levels
R5309	T8162	T8161	prep	by,determined
R5310	T8163	T8164	nmod	gas,chromatography
R5311	T8164	T8165	nmod	chromatography,analysis
R5312	T8165	T8162	pobj	analysis,by
R5313	T8166	T8164	punct	(,chromatography
R5314	T8167	T8164	appos	GC,chromatography
R5315	T8168	T8165	punct	),analysis
R5316	T8169	T8157	punct	", ",levels
R5317	T8170	T8157	prep	in,levels
R5318	T8171	T8170	pobj	plasma,in
R5319	T8172	T8171	cc	and,plasma
R5320	T8173	T8171	conj	tissues,plasma
R5321	T8174	T8171	prep	of,plasma
R5322	T8175	T8176	det	the,mice
R5323	T8176	T8174	pobj	mice,of
R5324	T8177	T8176	nmod	Abcg8,mice
R5325	T8178	T8177	punct	-,Abcg8
R5326	T8179	T8177	punct	/,Abcg8
R5327	T8180	T8177	punct	-,Abcg8
R5328	T8181	T8158	auxpass	are,shown
R5329	T8182	T8158	prep	in,shown
R5330	T8183	T8182	pobj	Table,in
R5331	T8184	T8183	nummod	2,Table
R5332	T8185	T8158	punct	.,shown
R5333	T8187	T8188	expl	There,were
R5334	T8189	T8190	det	no,differences
R5335	T8190	T8188	attr	differences,were
R5336	T8191	T8190	prep	in,differences
R5337	T8192	T8193	compound	tissue,weights
R5338	T8193	T8191	pobj	weights,in
R5339	T8194	T8190	prep	between,differences
R5340	T8195	T8196	amod	wild,types
R5341	T8196	T8194	pobj	types,between
R5342	T8197	T8196	punct	", ",types
R5343	T8198	T8196	conj	heterozygotes,types
R5344	T8199	T8198	cc	and,heterozygotes
R5345	T8200	T8198	conj	knockouts,heterozygotes
R5346	T8201	T8188	punct	.,were
R5347	T8203	T8204	compound	Plasma,cholesterol
R5348	T8204	T8205	compound	cholesterol,levels
R5349	T8205	T8206	nsubjpass	levels,decreased
R5350	T8207	T8205	prep	of,levels
R5351	T8208	T8209	det	the,mice
R5352	T8209	T8207	pobj	mice,of
R5353	T8210	T8209	amod	homozygous,mice
R5354	T8211	T8210	cc	and,homozygous
R5355	T8212	T8210	conj	heterozygous,homozygous
R5356	T8213	T8206	auxpass	were,decreased
R5357	T8214	T8206	prep	by,decreased
R5358	T8215	T8216	nummod	52,%
R5359	T8216	T8214	pobj	%,by
R5360	T8217	T8216	cc	and,%
R5361	T8218	T8219	nummod	26,%
R5362	T8219	T8216	conj	%,%
R5363	T8220	T8206	punct	", ",decreased
R5364	T8221	T8206	advmod	respectively,decreased
R5365	T8222	T8206	punct	", ",decreased
R5366	T8223	T8206	prep	compared,decreased
R5367	T8224	T8223	prep	to,compared
R5368	T8225	T8224	pobj	those,to
R5369	T8226	T8225	prep	of,those
R5370	T8227	T8228	amod	wild,type
R5371	T8228	T8230	compound	type,mice
R5372	T8229	T8228	punct	-,type
R5373	T8230	T8226	pobj	mice,of
R5374	T8231	T8232	punct	(,Table
R5375	T8232	T8206	parataxis	Table,decreased
R5376	T8233	T8234	nsubj	n,3
R5377	T8234	T8232	ccomp	3,Table
R5378	T8235	T8234	punct	=,3
R5379	T8236	T8237	det	all,groups
R5380	T8237	T8232	dep	groups,Table
R5381	T8238	T8232	punct	", ",Table
R5382	T8239	T8232	nummod	2,Table
R5383	T8240	T8232	punct	),Table
R5384	T8241	T8206	punct	.,decreased
R5385	T8243	T8244	compound	Plant,sterols
R5386	T8244	T8245	nsubj	sterols,were
R5387	T8246	T8247	advmod	almost,undetectable
R5388	T8247	T8245	acomp	undetectable,were
R5389	T8248	T8245	prep	in,were
R5390	T8249	T8250	amod	wild,type
R5391	T8250	T8252	nmod	type,mice
R5392	T8251	T8250	punct	-,type
R5393	T8252	T8248	pobj	mice,in
R5394	T8253	T8250	cc	and,type
R5395	T8254	T8250	conj	heterozygous,type
R5396	T8255	T8245	punct	", ",were
R5397	T8256	T8245	cc	but,were
R5398	T8257	T8258	auxpass	were,elevated
R5399	T8258	T8245	conj	elevated,were
R5400	T8259	T8258	advmod	significantly,elevated
R5401	T8260	T8258	prep	in,elevated
R5402	T8261	T8262	nmod	Abcg8,mice
R5403	T8262	T8260	pobj	mice,in
R5404	T8263	T8261	punct	-,Abcg8
R5405	T8264	T8261	punct	/,Abcg8
R5406	T8265	T8261	punct	-,Abcg8
R5407	T8266	T8245	punct	.,were
R5408	T8268	T8269	nmod	Plasma,levels
R5409	T8269	T8273	nsubj	levels,were
R5410	T8270	T8269	nmod	campesterol,levels
R5411	T8271	T8270	cc	and,campesterol
R5412	T8272	T8270	conj	sitosterol,campesterol
R5413	T8274	T8275	npadvmod	eight,higher
R5414	T8275	T8273	acomp	higher,were
R5415	T8276	T8274	punct	-,eight
R5416	T8277	T8274	cc	and,eight
R5417	T8278	T8274	conj	36-fold,eight
R5418	T8279	T8273	prep	in,were
R5419	T8280	T8281	nmod	Abcg8,mice
R5420	T8281	T8279	pobj	mice,in
R5421	T8282	T8280	punct	-,Abcg8
R5422	T8283	T8280	punct	/,Abcg8
R5423	T8284	T8280	punct	-,Abcg8
R5424	T8285	T8273	prep	compared,were
R5425	T8286	T8285	prep	to,compared
R5426	T8287	T8288	amod	wild,type
R5427	T8288	T8290	compound	type,mice
R5428	T8289	T8288	punct	-,type
R5429	T8290	T8286	pobj	mice,to
R5430	T8291	T8292	punct	(,mg
R5431	T8292	T8273	parataxis	mg,were
R5432	T8293	T8294	quantmod	8.76,2.10
R5433	T8294	T8292	nummod	2.10,mg
R5434	T8295	T8294	punct	±,2.10
R5435	T8296	T8294	cc	and,2.10
R5436	T8297	T8298	quantmod	18.52,5.03
R5437	T8298	T8294	conj	5.03,2.10
R5438	T8299	T8298	punct	±,5.03
R5439	T8300	T8301	punct	/,dL
R5440	T8301	T8292	prep	dL,mg
R5441	T8302	T8292	punct	", ",mg
R5442	T8303	T8292	advmod	respectively,mg
R5443	T8304	T8292	punct	", ",mg
R5444	T8305	T8292	cc	versus,mg
R5445	T8306	T8307	quantmod	1.14,0.69
R5446	T8307	T8309	nummod	0.69,mg
R5447	T8308	T8307	punct	±,0.69
R5448	T8309	T8292	conj	mg,mg
R5449	T8310	T8307	cc	and,0.69
R5450	T8311	T8312	quantmod	0.50,0.55
R5451	T8312	T8307	conj	0.55,0.69
R5452	T8313	T8312	punct	±,0.55
R5453	T8314	T8315	punct	/,dL
R5454	T8315	T8309	prep	dL,mg
R5455	T8316	T8292	punct	),mg
R5456	T8317	T8273	punct	.,were
R5457	T8319	T8320	expl	There,was
R5458	T8321	T8322	det	no,difference
R5459	T8322	T8320	attr	difference,was
R5460	T8323	T8322	amod	significant,difference
R5461	T8324	T8322	prep	in,difference
R5462	T8325	T8326	det	the,levels
R5463	T8326	T8324	pobj	levels,in
R5464	T8327	T8328	compound	plasma,sterol
R5465	T8328	T8326	compound	sterol,levels
R5466	T8329	T8328	compound	plant,sterol
R5467	T8330	T8320	prep	between,was
R5468	T8331	T8332	amod	heterozygous,mice
R5469	T8332	T8330	pobj	mice,between
R5470	T8333	T8331	cc	and,heterozygous
R5471	T8334	T8335	amod	wild,type
R5472	T8335	T8331	conj	type,heterozygous
R5473	T8336	T8335	punct	-,type
R5474	T8337	T8320	punct	.,was
R5475	T8339	T8340	compound	Liver,cholesterol
R5476	T8340	T8341	compound	cholesterol,content
R5477	T8341	T8342	nsubjpass	content,reduced
R5478	T8343	T8341	prep	of,content
R5479	T8344	T8345	det	the,mice
R5480	T8345	T8343	pobj	mice,of
R5481	T8346	T8345	nmod	Abcg8,mice
R5482	T8347	T8346	punct	-,Abcg8
R5483	T8348	T8346	punct	/,Abcg8
R5484	T8349	T8346	punct	-,Abcg8
R5485	T8350	T8342	auxpass	was,reduced
R5486	T8351	T8342	prep	by,reduced
R5487	T8352	T8353	punct	~,50
R5488	T8353	T8354	nummod	50,%
R5489	T8354	T8351	pobj	%,by
R5490	T8355	T8342	advcl	relative,reduced
R5491	T8356	T8355	prep	to,relative
R5492	T8357	T8358	amod	wild,type
R5493	T8358	T8360	compound	type,liver
R5494	T8359	T8358	punct	-,type
R5495	T8360	T8356	pobj	liver,to
R5496	T8361	T8362	punct	(,μg
R5497	T8362	T8342	parataxis	μg,reduced
R5498	T8363	T8364	quantmod	1202,264
R5499	T8364	T8362	nummod	264,μg
R5500	T8365	T8364	punct	±,264
R5501	T8366	T8367	punct	/,tissue
R5502	T8367	T8362	prep	tissue,μg
R5503	T8368	T8367	nmod	g,tissue
R5504	T8369	T8370	amod	wet,weight
R5505	T8370	T8367	compound	weight,tissue
R5506	T8371	T8362	punct	", ",μg
R5507	T8372	T8362	cc	versus,μg
R5508	T8373	T8374	quantmod	2280,310
R5509	T8374	T8376	nummod	310,μg
R5510	T8375	T8374	punct	±,310
R5511	T8376	T8362	conj	μg,μg
R5512	T8377	T8378	punct	/,tissue
R5513	T8378	T8376	prep	tissue,μg
R5514	T8379	T8378	nmod	g,tissue
R5515	T8380	T8381	amod	wet,weight
R5516	T8381	T8378	compound	weight,tissue
R5517	T8382	T8362	punct	", ",μg
R5518	T8383	T8362	advmod	respectively,μg
R5519	T8384	T8362	punct	),μg
R5520	T8385	T8342	punct	.,reduced
R5521	T8387	T8388	det	The,content
R5522	T8388	T8391	nsubjpass	content,offset
R5523	T8389	T8388	amod	reduced,content
R5524	T8390	T8388	compound	cholesterol,content
R5525	T8392	T8391	auxpass	was,offset
R5526	T8393	T8391	agent	by,offset
R5527	T8394	T8395	det	a,increase
R5528	T8395	T8393	pobj	increase,by
R5529	T8396	T8395	advmod	five-fold,increase
R5530	T8397	T8395	prep	in,increase
R5531	T8398	T8397	pobj	campesterol,in
R5532	T8399	T8395	cc	and,increase
R5533	T8400	T8401	det	a,increase
R5534	T8401	T8395	conj	increase,increase
R5535	T8402	T8401	advmod	22-fold,increase
R5536	T8403	T8401	prep	in,increase
R5537	T8404	T8403	pobj	sitosterol,in
R5538	T8405	T8406	punct	(,μg
R5539	T8406	T8401	parataxis	μg,increase
R5540	T8407	T8408	quantmod	233.9,48.1
R5541	T8408	T8406	nummod	48.1,μg
R5542	T8409	T8408	punct	±,48.1
R5543	T8410	T8408	cc	and,48.1
R5544	T8411	T8412	quantmod	376.3,96.1
R5545	T8412	T8408	conj	96.1,48.1
R5546	T8413	T8412	punct	±,96.1
R5547	T8414	T8415	punct	/,tissue
R5548	T8415	T8406	prep	tissue,μg
R5549	T8416	T8415	nmod	g,tissue
R5550	T8417	T8418	amod	wet,weight
R5551	T8418	T8415	compound	weight,tissue
R5552	T8419	T8406	punct	", ",μg
R5553	T8420	T8406	advmod	respectively,μg
R5554	T8421	T8406	punct	),μg
R5555	T8422	T8395	prep	in,increase
R5556	T8423	T8424	nmod	Abcg8,knockout
R5557	T8424	T8428	compound	knockout,mice
R5558	T8425	T8423	punct	-,Abcg8
R5559	T8426	T8423	punct	/,Abcg8
R5560	T8427	T8423	punct	-,Abcg8
R5561	T8428	T8422	pobj	mice,in
R5562	T8429	T8395	prep	compared,increase
R5563	T8430	T8429	prep	to,compared
R5564	T8431	T8432	amod	wild,type
R5565	T8432	T8434	compound	type,mice
R5566	T8433	T8432	punct	-,type
R5567	T8434	T8430	pobj	mice,to
R5568	T8435	T8436	punct	(,Table
R5569	T8436	T8391	parataxis	Table,offset
R5570	T8437	T8438	quantmod	52.2,15.0
R5571	T8438	T8440	nummod	15.0,μg
R5572	T8439	T8438	punct	±,15.0
R5573	T8440	T8436	dep	μg,Table
R5574	T8441	T8438	cc	and,15.0
R5575	T8442	T8443	quantmod	16.7,8.6
R5576	T8443	T8438	conj	8.6,15.0
R5577	T8444	T8443	punct	±,8.6
R5578	T8445	T8446	punct	/,tissue
R5579	T8446	T8440	prep	tissue,μg
R5580	T8447	T8446	nmod	g,tissue
R5581	T8448	T8449	amod	wet,weight
R5582	T8449	T8446	compound	weight,tissue
R5583	T8450	T8440	punct	", ",μg
R5584	T8451	T8440	advmod	respectively,μg
R5585	T8452	T8436	punct	", ",Table
R5586	T8453	T8436	nummod	2,Table
R5587	T8454	T8436	punct	),Table
R5588	T8455	T8391	punct	.,offset
R5589	T8457	T8458	expl	There,were
R5590	T8459	T8460	det	no,differences
R5591	T8460	T8458	attr	differences,were
R5592	T8461	T8460	amod	significant,differences
R5593	T8462	T8460	prep	of,differences
R5594	T8463	T8464	nmod	liver,cholesterol
R5595	T8464	T8465	nmod	cholesterol,contents
R5596	T8465	T8462	pobj	contents,of
R5597	T8466	T8464	cc	or,cholesterol
R5598	T8467	T8468	compound	plant,sterol
R5599	T8468	T8464	conj	sterol,cholesterol
R5600	T8469	T8460	prep	between,differences
R5601	T8470	T8471	nmod	Abcg8,mice
R5602	T8471	T8469	pobj	mice,between
R5603	T8472	T8470	punct	+,Abcg8
R5604	T8473	T8470	punct	/,Abcg8
R5605	T8474	T8470	punct	+,Abcg8
R5606	T8475	T8470	cc	and,Abcg8
R5607	T8476	T8470	conj	Abcg8,Abcg8
R5608	T8477	T8476	punct	+,Abcg8
R5609	T8478	T8476	punct	/,Abcg8
R5610	T8479	T8476	punct	-,Abcg8
R5611	T8480	T8458	punct	.,were
R5612	T8482	T8483	compound	Spleen,sterol
R5613	T8483	T8484	compound	sterol,contents
R5614	T8484	T8485	nsubj	contents,reflected
R5615	T8486	T8487	det	the,profiles
R5616	T8487	T8485	dobj	profiles,reflected
R5617	T8488	T8487	compound	liver,profiles
R5618	T8489	T8490	punct	(,Table
R5619	T8490	T8485	parataxis	Table,reflected
R5620	T8491	T8490	nummod	2,Table
R5621	T8492	T8490	punct	),Table
R5622	T8493	T8485	punct	.,reflected
R5623	T8495	T8496	det	No,differences
R5624	T8496	T8498	nsubjpass	differences,observed
R5625	T8497	T8496	amod	significant,differences
R5626	T8499	T8496	prep	in,differences
R5627	T8500	T8501	compound	brain,contents
R5628	T8501	T8499	pobj	contents,in
R5629	T8502	T8501	compound	sterol,contents
R5630	T8503	T8498	auxpass	were,observed
R5631	T8504	T8498	punct	", ",observed
R5632	T8505	T8506	mark	as,expected
R5633	T8506	T8498	advcl	expected,observed
R5634	T8507	T8506	aux	would,expected
R5635	T8508	T8506	auxpass	be,expected
R5636	T8509	T8498	punct	", ",observed
R5637	T8510	T8511	mark	since,is
R5638	T8511	T8498	advcl	is,observed
R5639	T8512	T8513	det	the,barrier
R5640	T8513	T8511	nsubj	barrier,is
R5641	T8514	T8515	compound	blood,brain
R5642	T8515	T8513	compound	brain,barrier
R5643	T8516	T8511	acomp	intact,is
R5644	T8517	T8511	cc	and,is
R5645	T8518	T8511	conj	prevents,is
R5646	T8519	T8518	dobj	entry,prevents
R5647	T8520	T8519	prep	of,entry
R5648	T8521	T8522	det	these,sterols
R5649	T8522	T8520	pobj	sterols,of
R5650	T8523	T8518	prep	in,prevents
R5651	T8524	T8523	pobj	sitosterolemia,in
R5652	T8525	T8526	punct	(,Table
R5653	T8526	T8498	parataxis	Table,observed
R5654	T8527	T8526	nummod	2,Table
R5655	T8528	T8526	punct	),Table
R5656	T8529	T8498	punct	.,observed
R5657	T8531	T8532	expl	There,were
R5658	T8533	T8532	advmod	only,were
R5659	T8534	T8532	attr	traces,were
R5660	T8535	T8534	prep	of,traces
R5661	T8536	T8537	compound	plant,sterols
R5662	T8537	T8535	pobj	sterols,of
R5663	T8538	T8532	prep	in,were
R5664	T8539	T8540	det	the,brains
R5665	T8540	T8538	pobj	brains,in
R5666	T8541	T8540	prep	from,brains
R5667	T8542	T8543	compound	knockout,animals
R5668	T8543	T8541	pobj	animals,from
R5669	T8544	T8532	punct	", ",were
R5670	T8545	T8532	advcl	reflecting,were
R5671	T8546	T8547	compound	blood,contamination
R5672	T8547	T8545	dobj	contamination,reflecting
R5673	T8548	T8547	prep	during,contamination
R5674	T8549	T8550	compound	tissue,harvesting
R5675	T8550	T8548	pobj	harvesting,during
R5676	T8551	T8532	punct	.,were
R5677	T8553	T8554	advmod	Interestingly,are
R5678	T8555	T8554	punct	", ",are
R5679	T8556	T8557	det	the,majority
R5680	T8557	T8554	nsubj	majority,are
R5681	T8558	T8557	prep	of,majority
R5682	T8559	T8560	det	the,sterols
R5683	T8560	T8558	pobj	sterols,of
R5684	T8561	T8560	amod	elevated,sterols
R5685	T8562	T8560	compound	tissue,sterols
R5686	T8563	T8560	compound	plant,sterols
R5687	T8564	T8554	acomp	unesterified,are
R5688	T8565	T8554	punct	.,are
R5689	T8567	T8568	det	The,livers
R5690	T8568	T8569	nsubj	livers,have
R5691	T8570	T8568	prep	of,livers
R5692	T8571	T8572	nmod	Abcg8,+
R5693	T8572	T8575	punct	+,mice
R5694	T8573	T8572	punct	+,+
R5695	T8574	T8572	punct	/,+
R5696	T8575	T8570	pobj	mice,of
R5697	T8576	T8572	punct	", ",+
R5698	T8577	T8578	punct	+,-
R5699	T8578	T8572	conj	-,+
R5700	T8579	T8578	punct	/,-
R5701	T8580	T8578	cc	and,-
R5702	T8581	T8582	punct	-,-
R5703	T8582	T8578	conj	-,-
R5704	T8583	T8582	punct	/,-
R5705	T8584	T8585	advmod	relatively,similar
R5706	T8585	T8586	amod	similar,levels
R5707	T8586	T8569	dobj	levels,have
R5708	T8587	T8586	prep	of,levels
R5709	T8588	T8589	amod	esterified,cholesterol
R5710	T8589	T8587	pobj	cholesterol,of
R5711	T8590	T8591	punct	(,2a
R5712	T8591	T8569	parataxis	2a,have
R5713	T8592	T8591	compound	Figure,2a
R5714	T8593	T8591	punct	),2a
R5715	T8594	T8569	punct	.,have
R5716	T8596	T8597	advmod	However,is
R5717	T8598	T8597	punct	", ",is
R5718	T8599	T8597	expl	there,is
R5719	T8600	T8601	amod	little,esterification
R5720	T8601	T8597	attr	esterification,is
R5721	T8602	T8603	mark	if,any
R5722	T8603	T8600	parataxis	any,little
R5723	T8604	T8601	prep	of,esterification
R5724	T8605	T8604	pobj	sitosterol,of
R5725	T8606	T8605	cc	or,sitosterol
R5726	T8607	T8605	conj	campesterol,sitosterol
R5727	T8608	T8609	punct	(,2c
R5728	T8609	T8601	parataxis	2c,esterification
R5729	T8610	T8609	nmod	Figure,2c
R5730	T8611	T8609	nmod	2b,2c
R5731	T8612	T8609	punct	",",2c
R5732	T8613	T8609	punct	),2c
R5733	T8614	T8597	advcl	consistent,is
R5734	T8615	T8614	prep	with,consistent
R5735	T8616	T8617	amod	previous,findings
R5736	T8617	T8615	pobj	findings,with
R5737	T8618	T8619	punct	[,38
R5738	T8619	T8597	parataxis	38,is
R5739	T8620	T8619	nummod	37,38
R5740	T8621	T8619	punct	",",38
R5741	T8622	T8619	punct	],38
R5742	T8623	T8597	punct	.,is
R5743	T8625	T8626	advmod	Thus,are
R5744	T8627	T8626	nsubj	all,are
R5745	T8628	T8627	prep	of,all
R5746	T8629	T8630	det	the,expansion
R5747	T8630	T8628	pobj	expansion,of
R5748	T8631	T8630	prep	of,expansion
R5749	T8632	T8633	compound	tissue,pools
R5750	T8633	T8631	pobj	pools,of
R5751	T8634	T8633	compound	sterol,pools
R5752	T8635	T8626	prep	as,are
R5753	T8636	T8637	amod	free,sterols
R5754	T8637	T8635	pobj	sterols,as
R5755	T8638	T8626	punct	.,are
R5756	T8640	T8641	compound	FPLC,analyses
R5757	T8641	T8642	nsubjpass	analyses,performed
R5758	T8643	T8641	prep	for,analyses
R5759	T8644	T8643	pobj	sterols,for
R5760	T8645	T8644	punct	(,sterols
R5761	T8646	T8644	acl	measured,sterols
R5762	T8647	T8646	advmod	enzymatically,measured
R5763	T8648	T8646	cc	and,measured
R5764	T8649	T8650	advmod	thus,reflecting
R5765	T8650	T8646	conj	reflecting,measured
R5766	T8651	T8652	amod	total,sterols
R5767	T8652	T8650	dobj	sterols,reflecting
R5768	T8653	T8644	punct	),sterols
R5769	T8654	T8644	cc	and,sterols
R5770	T8655	T8644	conj	triglycerides,sterols
R5771	T8656	T8642	auxpass	were,performed
R5772	T8657	T8642	prep	on,performed
R5773	T8658	T8659	compound	plasma,samples
R5774	T8659	T8657	pobj	samples,on
R5775	T8660	T8659	prep	from,samples
R5776	T8661	T8662	amod	fasted,animals
R5777	T8662	T8660	pobj	animals,from
R5778	T8663	T8662	acl	fed,animals
R5779	T8664	T8665	det	a,diet
R5780	T8665	T8663	dobj	diet,fed
R5781	T8666	T8665	amod	regular,diet
R5782	T8667	T8665	compound	chow,diet
R5783	T8668	T8642	punct	.,performed
R5784	T8670	T8671	det	No,differences
R5785	T8671	T8673	nsubjpass	differences,observed
R5786	T8672	T8671	amod	significant,differences
R5787	T8674	T8673	auxpass	were,observed
R5788	T8675	T8673	prep	for,observed
R5789	T8676	T8677	det	the,profiles
R5790	T8677	T8675	pobj	profiles,for
R5791	T8678	T8677	compound	sterol,profiles
R5792	T8679	T8680	punct	(,3a
R5793	T8680	T8673	parataxis	3a,observed
R5794	T8681	T8680	compound	Figure,3a
R5795	T8682	T8680	punct	),3a
R5796	T8683	T8673	punct	", ",observed
R5797	T8684	T8673	cc	but,observed
R5798	T8685	T8686	advmod	surprisingly,had
R5799	T8686	T8673	conj	had,observed
R5800	T8687	T8686	punct	", ",had
R5801	T8688	T8689	det	the,mice
R5802	T8689	T8686	nsubj	mice,had
R5803	T8690	T8691	nmod	Abcg8,knockout
R5804	T8691	T8689	compound	knockout,mice
R5805	T8692	T8690	punct	-,Abcg8
R5806	T8693	T8690	punct	/,Abcg8
R5807	T8694	T8690	punct	-,Abcg8
R5808	T8695	T8696	det	a,level
R5809	T8696	T8686	dobj	level,had
R5810	T8697	T8698	advmod	significantly,higher
R5811	T8698	T8696	amod	higher,level
R5812	T8699	T8696	compound	triglyceride,level
R5813	T8700	T8696	prep	in,level
R5814	T8701	T8702	det	the,fractions
R5815	T8702	T8700	pobj	fractions,in
R5816	T8703	T8704	compound	LDL,lipoprotein
R5817	T8704	T8702	compound	lipoprotein,fractions
R5818	T8705	T8696	prep	compared,level
R5819	T8706	T8705	prep	to,compared
R5820	T8707	T8708	amod	wild,type
R5821	T8708	T8710	nmod	type,mice
R5822	T8709	T8708	punct	-,type
R5823	T8710	T8706	pobj	mice,to
R5824	T8711	T8708	cc	and,type
R5825	T8712	T8708	conj	heterozygous,type
R5826	T8713	T8714	punct	(,3b
R5827	T8714	T8686	parataxis	3b,had
R5828	T8715	T8714	compound	Figure,3b
R5829	T8716	T8714	punct	),3b
R5830	T8717	T8686	punct	.,had
R5831	T8719	T8720	det	The,size
R5832	T8720	T8721	nsubj	size,was
R5833	T8722	T8720	prep	of,size
R5834	T8723	T8724	det	this,peak
R5835	T8724	T8722	pobj	peak,of
R5836	T8725	T8721	acomp	variable,was
R5837	T8726	T8721	prep	between,was
R5838	T8727	T8728	amod	different,analyses
R5839	T8728	T8726	pobj	analyses,between
R5840	T8729	T8728	compound	littermate,analyses
R5841	T8730	T8721	punct	", ",was
R5842	T8731	T8721	cc	but,was
R5843	T8732	T8721	conj	is,was
R5844	T8733	T8732	advmod	always,is
R5845	T8734	T8732	acomp	increased,is
R5846	T8735	T8732	prep	in,is
R5847	T8736	T8737	det	the,animals
R5848	T8737	T8735	pobj	animals,in
R5849	T8738	T8737	compound	knockout,animals
R5850	T8739	T8721	punct	.,was
R5851	T8741	T8742	amod	Total,levels
R5852	T8742	T8745	nsubj	levels,were
R5853	T8743	T8744	compound	plasma,triglyceride
R5854	T8744	T8742	compound	triglyceride,levels
R5855	T8746	T8742	prep	of,levels
R5856	T8747	T8748	det	the,mice
R5857	T8748	T8746	pobj	mice,of
R5858	T8749	T8748	nmod	Abcg8,mice
R5859	T8750	T8749	punct	-,Abcg8
R5860	T8751	T8749	punct	/,Abcg8
R5861	T8752	T8749	punct	-,Abcg8
R5862	T8753	T8754	advmod	slightly,higher
R5863	T8754	T8745	acomp	higher,were
R5864	T8755	T8756	punct	(,mg
R5865	T8756	T8745	parataxis	mg,were
R5866	T8757	T8758	quantmod	79.2,14
R5867	T8758	T8756	nummod	14,mg
R5868	T8759	T8758	punct	±,14
R5869	T8760	T8761	punct	/,dL
R5870	T8761	T8756	prep	dL,mg
R5871	T8762	T8756	prep	compared,mg
R5872	T8763	T8762	prep	to,compared
R5873	T8764	T8765	det	the,mg
R5874	T8765	T8763	pobj	mg,to
R5875	T8766	T8767	amod	wild,type
R5876	T8767	T8765	nmod	type,mg
R5877	T8768	T8769	quantmod	63.4,13
R5878	T8769	T8765	nummod	13,mg
R5879	T8770	T8769	punct	±,13
R5880	T8771	T8772	punct	/,dL
R5881	T8772	T8765	prep	dL,mg
R5882	T8773	T8765	cc	and,mg
R5883	T8774	T8775	nmod	heterozygotes,mg
R5884	T8775	T8765	conj	mg,mg
R5885	T8776	T8777	quantmod	46.6,12
R5886	T8777	T8775	nummod	12,mg
R5887	T8778	T8777	punct	±,12
R5888	T8779	T8780	punct	/,dL
R5889	T8780	T8775	prep	dL,mg
R5890	T8781	T8756	punct	", ",mg
R5891	T8782	T8783	nsubj	n,3
R5892	T8783	T8756	ccomp	3,mg
R5893	T8784	T8783	punct	=,3
R5894	T8785	T8783	prep	for,3
R5895	T8786	T8787	det	all,genotypes
R5896	T8787	T8785	pobj	genotypes,for
R5897	T8788	T8756	punct	),mg
R5898	T8789	T8745	punct	.,were
R5899	T8791	T8792	amod	Preliminary,analyses
R5900	T8792	T8793	nsubj	analyses,show
R5901	T8794	T8792	prep	of,analyses
R5902	T8795	T8794	pobj	proteins,of
R5903	T8796	T8795	prep	in,proteins
R5904	T8797	T8798	det	the,fractions
R5905	T8798	T8796	pobj	fractions,in
R5906	T8799	T8798	amod	isolated,fractions
R5907	T8800	T8798	compound	FPLC,fractions
R5908	T8801	T8793	aux	did,show
R5909	T8802	T8793	neg	not,show
R5910	T8803	T8804	det	any,changes
R5911	T8804	T8793	dobj	changes,show
R5912	T8805	T8804	amod	qualitative,changes
R5913	T8806	T8804	prep	in,changes
R5914	T8807	T8808	compound	apolipoproteins,B
R5915	T8808	T8806	pobj	B,in
R5916	T8809	T8808	punct	", ",B
R5917	T8810	T8808	conj	E,B
R5918	T8811	T8810	punct	", ",E
R5919	T8812	T8810	conj	AI,E
R5920	T8813	T8812	punct	", ",AI
R5921	T8814	T8812	cc	or,AI
R5922	T8815	T8812	conj	AII,AI
R5923	T8816	T8793	punct	.,show
R5924	T8818	T8819	det	The,significance
R5925	T8819	T8820	nsubj	significance,remains
R5926	T8821	T8819	prep	of,significance
R5927	T8822	T8823	det	this,peak
R5928	T8823	T8821	pobj	peak,of
R5929	T8824	T8825	npadvmod	triglyceride,rich
R5930	T8825	T8823	amod	rich,peak
R5931	T8826	T8820	acomp	unclear,remains
R5932	T8827	T8820	prep	at,remains
R5933	T8828	T8827	amod	present,at
R5934	T8829	T8820	punct	.,remains
R5935	T9063	T9064	compound	Liver,expression
R5936	T9064	T9066	nsubj	expression,change
R5937	T9065	T9064	compound	gene,expression
R5938	T9067	T9064	cc	and,expression
R5939	T9068	T9069	compound	enzyme,activity
R5940	T9069	T9064	conj	activity,expression
R5941	T9070	T9066	prep	in,change
R5942	T9071	T9072	compound	Abcg8,sterolin
R5943	T9072	T9074	npadvmod	sterolin,deficient
R5944	T9073	T9072	punct	/,sterolin
R5945	T9074	T9077	amod	deficient,mice
R5946	T9075	T9072	punct	-,sterolin
R5947	T9076	T9072	nummod	2,sterolin
R5948	T9077	T9070	pobj	mice,in
R5949	T9079	T9080	aux	To,investigate
R5950	T9080	T9081	advcl	investigate,performed
R5951	T9082	T9083	det	the,effects
R5952	T9083	T9080	dobj	effects,investigate
R5953	T9084	T9083	prep	of,effects
R5954	T9085	T9086	det	a,deficiency
R5955	T9086	T9084	pobj	deficiency,of
R5956	T9087	T9086	prep	of,deficiency
R5957	T9088	T9089	compound	Abcg8,sterolin
R5958	T9089	T9087	pobj	sterolin,of
R5959	T9090	T9089	punct	/,sterolin
R5960	T9091	T9089	punct	-,sterolin
R5961	T9092	T9089	nummod	2,sterolin
R5962	T9093	T9083	prep	on,effects
R5963	T9094	T9095	det	the,genes
R5964	T9095	T9093	pobj	genes,on
R5965	T9096	T9097	dep	that,regulate
R5966	T9097	T9095	relcl	regulate,genes
R5967	T9098	T9099	compound	sterol,metabolism
R5968	T9099	T9097	dobj	metabolism,regulate
R5969	T9100	T9081	punct	", ",performed
R5970	T9101	T9102	amod	quantitative,PCR
R5971	T9102	T9081	nsubjpass	PCR,performed
R5972	T9103	T9102	compound	RT,PCR
R5973	T9104	T9102	punct	-,PCR
R5974	T9105	T9081	auxpass	was,performed
R5975	T9106	T9081	advcl	looking,performed
R5976	T9107	T9106	prep	at,looking
R5977	T9108	T9109	det	the,levels
R5978	T9109	T9107	pobj	levels,at
R5979	T9110	T9109	compound	expression,levels
R5980	T9111	T9109	prep	of,levels
R5981	T9112	T9113	nmod	Abcg5,mRNA
R5982	T9113	T9111	pobj	mRNA,of
R5983	T9114	T9112	punct	", ",Abcg5
R5984	T9115	T9112	conj	Abcg8,Abcg5
R5985	T9116	T9115	punct	", ",Abcg8
R5986	T9117	T9115	conj	Hmgr,Abcg8
R5987	T9118	T9117	punct	", ",Hmgr
R5988	T9119	T9117	conj	Cyp7a1,Hmgr
R5989	T9120	T9119	punct	", ",Cyp7a1
R5990	T9121	T9119	conj	Abca1,Cyp7a1
R5991	T9122	T9121	punct	", ",Abca1
R5992	T9123	T9121	conj	Mdr2,Abca1
R5993	T9124	T9123	punct	", ",Mdr2
R5994	T9125	T9123	conj	Lxr,Mdr2
R5995	T9126	T9125	punct	", ",Lxr
R5996	T9127	T9128	compound	Srebp,1c
R5997	T9128	T9125	conj	1c,Lxr
R5998	T9129	T9128	punct	-,1c
R5999	T9130	T9128	punct	", ",1c
R6000	T9131	T9128	cc	and,1c
R6001	T9132	T9128	conj	Srebp,1c
R6002	T9133	T9132	punct	-,Srebp
R6003	T9134	T9132	nummod	2,Srebp
R6004	T9135	T9109	prep	in,levels
R6005	T9136	T9137	det	the,livers
R6006	T9137	T9135	pobj	livers,in
R6007	T9138	T9137	prep	of,livers
R6008	T9139	T9138	pobj	mice,of
R6009	T9140	T9139	acl	fed,mice
R6010	T9141	T9142	det	a,diet
R6011	T9142	T9140	dobj	diet,fed
R6012	T9143	T9142	amod	regular,diet
R6013	T9144	T9142	compound	chow,diet
R6014	T9145	T9146	punct	(,4a
R6015	T9146	T9081	parataxis	4a,performed
R6016	T9147	T9146	compound	Figure,4a
R6017	T9148	T9146	punct	),4a
R6018	T9149	T9081	punct	.,performed
R6019	T9151	T9152	mark	As,expected
R6020	T9152	T9153	advcl	expected,were
R6021	T9154	T9153	punct	", ",were
R6022	T9155	T9156	compound	Abcg8,mRNA
R6023	T9156	T9157	compound	mRNA,levels
R6024	T9157	T9153	nsubj	levels,were
R6025	T9158	T9157	compound	expression,levels
R6026	T9159	T9153	acomp	undetectable,were
R6027	T9160	T9153	prep	in,were
R6028	T9161	T9162	det	the,mice
R6029	T9162	T9160	pobj	mice,in
R6030	T9163	T9162	nmod	Abcg8,mice
R6031	T9164	T9163	punct	-,Abcg8
R6032	T9165	T9163	punct	/,Abcg8
R6033	T9166	T9163	punct	-,Abcg8
R6034	T9167	T9153	cc	and,were
R6035	T9168	T9169	auxpass	were,reduced
R6036	T9169	T9153	conj	reduced,were
R6037	T9170	T9169	prep	by,reduced
R6038	T9171	T9172	punct	~,50
R6039	T9172	T9173	nummod	50,%
R6040	T9173	T9170	pobj	%,by
R6041	T9174	T9169	prep	in,reduced
R6042	T9175	T9176	det	the,mice
R6043	T9176	T9174	pobj	mice,in
R6044	T9177	T9176	amod	heterozygous,mice
R6045	T9178	T9169	punct	", ",reduced
R6046	T9179	T9169	advcl	relative,reduced
R6047	T9180	T9179	prep	to,relative
R6048	T9181	T9182	amod	wild,type
R6049	T9182	T9184	compound	type,mice
R6050	T9183	T9182	punct	-,type
R6051	T9184	T9180	pobj	mice,to
R6052	T9185	T9153	punct	.,were
R6053	T9187	T9188	advmod	Interestingly,reduced
R6054	T9189	T9188	punct	", ",reduced
R6055	T9190	T9188	prep	by,reduced
R6056	T9191	T9192	amod	quantitative,PCR
R6057	T9192	T9190	pobj	PCR,by
R6058	T9193	T9192	compound	RT,PCR
R6059	T9194	T9192	punct	-,PCR
R6060	T9195	T9188	punct	", ",reduced
R6061	T9196	T9197	det	the,expression
R6062	T9197	T9188	nsubjpass	expression,reduced
R6063	T9198	T9197	compound	mRNA,expression
R6064	T9199	T9197	prep	of,expression
R6065	T9200	T9199	pobj	Abcg5,of
R6066	T9201	T9197	prep	in,expression
R6067	T9202	T9203	det	the,mice
R6068	T9203	T9201	pobj	mice,in
R6069	T9204	T9203	amod	knock,mice
R6070	T9205	T9204	punct	-,knock
R6071	T9206	T9204	prt	out,knock
R6072	T9207	T9188	auxpass	was,reduced
R6073	T9208	T9188	advmod	also,reduced
R6074	T9209	T9188	prep	by,reduced
R6075	T9210	T9211	amod	more,60
R6076	T9211	T9213	nummod	60,%
R6077	T9212	T9211	quantmod	than,60
R6078	T9213	T9209	pobj	%,by
R6079	T9214	T9188	prep	compared,reduced
R6080	T9215	T9214	prep	to,compared
R6081	T9216	T9217	det	the,mice
R6082	T9217	T9215	pobj	mice,to
R6083	T9218	T9219	amod	wild,type
R6084	T9219	T9217	compound	type,mice
R6085	T9220	T9219	punct	-,type
R6086	T9221	T9188	punct	", ",reduced
R6087	T9222	T9223	mark	although,noted
R6088	T9223	T9188	advcl	noted,reduced
R6089	T9224	T9225	det	no,changes
R6090	T9225	T9223	nsubjpass	changes,noted
R6091	T9226	T9223	auxpass	were,noted
R6092	T9227	T9223	prep	in,noted
R6093	T9228	T9229	det	the,mice
R6094	T9229	T9227	pobj	mice,in
R6095	T9230	T9229	amod	heterozygous,mice
R6096	T9231	T9188	punct	.,reduced
R6097	T9233	T9234	nsubjpass	Expression,decreased
R6098	T9235	T9233	prep	of,Expression
R6099	T9236	T9237	compound	HMG,CoA
R6100	T9237	T9239	compound	CoA,reductase
R6101	T9238	T9237	punct	-,CoA
R6102	T9239	T9240	compound	reductase,mRNA
R6103	T9240	T9235	pobj	mRNA,of
R6104	T9241	T9234	auxpass	was,decreased
R6105	T9242	T9234	prep	by,decreased
R6106	T9243	T9244	punct	~,50
R6107	T9244	T9245	nummod	50,%
R6108	T9245	T9242	pobj	%,by
R6109	T9246	T9245	cc	and,%
R6110	T9247	T9248	punct	~,80
R6111	T9248	T9249	nummod	80,%
R6112	T9249	T9245	conj	%,%
R6113	T9250	T9234	prep	in,decreased
R6114	T9251	T9252	det	the,mice
R6115	T9252	T9250	pobj	mice,in
R6116	T9253	T9252	nmod	heterozygote,mice
R6117	T9254	T9253	cc	and,heterozygote
R6118	T9255	T9253	conj	knockout,heterozygote
R6119	T9256	T9234	advmod	respectively,decreased
R6120	T9257	T9234	punct	", ",decreased
R6121	T9258	T9234	prep	in,decreased
R6122	T9259	T9258	pobj	keeping,in
R6123	T9260	T9259	prep	with,keeping
R6124	T9261	T9262	amod	limited,observations
R6125	T9262	T9260	pobj	observations,with
R6126	T9263	T9262	prep	in,observations
R6127	T9264	T9265	amod	human,patients
R6128	T9265	T9263	pobj	patients,in
R6129	T9266	T9265	prep	with,patients
R6130	T9267	T9268	det	this,disorder
R6131	T9268	T9266	pobj	disorder,with
R6132	T9269	T9270	punct	[,7
R6133	T9270	T9234	parataxis	7,decreased
R6134	T9271	T9270	punct	],7
R6135	T9272	T9234	punct	.,decreased
R6136	T9274	T9275	aux	To,verify
R6137	T9275	T9276	advcl	verify,analyzed
R6138	T9277	T9278	mark	whether,resulted
R6139	T9278	T9275	ccomp	resulted,verify
R6140	T9279	T9280	det	the,changes
R6141	T9280	T9278	nsubj	changes,resulted
R6142	T9281	T9280	compound	mRNA,changes
R6143	T9282	T9278	prep	in,resulted
R6144	T9283	T9282	pobj	alteration,in
R6145	T9284	T9283	prep	of,alteration
R6146	T9285	T9286	det	the,changes
R6147	T9286	T9284	pobj	changes,of
R6148	T9287	T9288	compound	enzyme,activity
R6149	T9288	T9286	compound	activity,changes
R6150	T9289	T9276	punct	", ",analyzed
R6151	T9290	T9291	compound	liver,samples
R6152	T9291	T9276	nsubjpass	samples,analyzed
R6153	T9292	T9276	auxpass	were,analyzed
R6154	T9293	T9276	prep	for,analyzed
R6155	T9294	T9295	compound	HMG,CoA
R6156	T9295	T9297	compound	CoA,reductase
R6157	T9296	T9295	punct	-,CoA
R6158	T9297	T9298	compound	reductase,activity
R6159	T9298	T9293	pobj	activity,for
R6160	T9299	T9298	cc	and,activity
R6161	T9300	T9301	compound	Cyp7a1,activity
R6162	T9301	T9298	conj	activity,activity
R6163	T9302	T9303	punct	(,4b
R6164	T9303	T9276	parataxis	4b,analyzed
R6165	T9304	T9303	compound	Figure,4b
R6166	T9305	T9303	punct	),4b
R6167	T9306	T9276	punct	.,analyzed
R6168	T9308	T9309	compound	HMG,CoA
R6169	T9309	T9311	compound	CoA,reductase
R6170	T9310	T9309	punct	-,CoA
R6171	T9311	T9312	compound	reductase,activity
R6172	T9312	T9313	nsubjpass	activity,reduced
R6173	T9314	T9313	auxpass	was,reduced
R6174	T9315	T9313	prep	by,reduced
R6175	T9316	T9317	nummod	30,%
R6176	T9317	T9315	pobj	%,by
R6177	T9318	T9317	cc	and,%
R6178	T9319	T9320	nummod	60,%
R6179	T9320	T9317	conj	%,%
R6180	T9321	T9313	prep	in,reduced
R6181	T9322	T9323	det	the,mice
R6182	T9323	T9321	pobj	mice,in
R6183	T9324	T9323	nmod	Abcg8,mice
R6184	T9325	T9324	punct	+,Abcg8
R6185	T9326	T9324	punct	/,Abcg8
R6186	T9327	T9324	punct	-,Abcg8
R6187	T9328	T9324	cc	and,Abcg8
R6188	T9329	T9324	conj	Abcg8,Abcg8
R6189	T9330	T9329	punct	-,Abcg8
R6190	T9331	T9329	punct	/,Abcg8
R6191	T9332	T9329	punct	-,Abcg8
R6192	T9333	T9313	punct	", ",reduced
R6193	T9334	T9313	advmod	respectively,reduced
R6194	T9335	T9336	punct	(,4b
R6195	T9336	T9313	parataxis	4b,reduced
R6196	T9337	T9336	compound	Figure,4b
R6197	T9338	T9336	punct	),4b
R6198	T9339	T9313	punct	", ",reduced
R6199	T9340	T9313	cc	and,reduced
R6200	T9341	T9342	advmod	thus,reflected
R6201	T9342	T9313	conj	reflected,reduced
R6202	T9343	T9344	det	the,changes
R6203	T9344	T9342	dobj	changes,reflected
R6204	T9345	T9344	prep	in,changes
R6205	T9346	T9347	compound	mRNA,expression
R6206	T9347	T9345	pobj	expression,in
R6207	T9348	T9313	punct	.,reduced
R6208	T9350	T9351	prep	In,decreased
R6209	T9352	T9350	pobj	contrast,In
R6210	T9353	T9351	punct	", ",decreased
R6211	T9354	T9355	mark	although,were
R6212	T9355	T9351	advcl	were,decreased
R6213	T9356	T9357	det	the,levels
R6214	T9357	T9355	nsubj	levels,were
R6215	T9358	T9359	compound	Cyp7a1,mRNA
R6216	T9359	T9357	compound	mRNA,levels
R6217	T9360	T9357	compound	expression,levels
R6218	T9361	T9362	advmod	essentially,unchanged
R6219	T9362	T9355	acomp	unchanged,were
R6220	T9363	T9355	prep	in,were
R6221	T9364	T9365	det	the,mouse
R6222	T9365	T9363	pobj	mouse,in
R6223	T9366	T9365	compound	knockout,mouse
R6224	T9367	T9351	punct	", ",decreased
R6225	T9368	T9369	compound	Cyp7a1,activity
R6226	T9369	T9351	nsubjpass	activity,decreased
R6227	T9370	T9351	auxpass	was,decreased
R6228	T9371	T9351	advmod	significantly,decreased
R6229	T9372	T9351	prep	by,decreased
R6230	T9373	T9374	nummod	37,%
R6231	T9374	T9372	pobj	%,by
R6232	T9375	T9376	punct	(,0.01
R6233	T9376	T9351	parataxis	0.01,decreased
R6234	T9377	T9376	nsubj	P,0.01
R6235	T9378	T9376	punct	<,0.01
R6236	T9379	T9376	punct	),0.01
R6237	T9380	T9351	punct	.,decreased
R6238	T9382	T9383	prep	In,decreased
R6239	T9384	T9385	det	the,mice
R6240	T9385	T9382	pobj	mice,In
R6241	T9386	T9385	amod	heterozygous,mice
R6242	T9387	T9383	punct	", ",decreased
R6243	T9388	T9389	preconj	both,mRNA
R6244	T9389	T9383	nsubjpass	mRNA,decreased
R6245	T9390	T9389	det	the,mRNA
R6246	T9391	T9389	cc	and,mRNA
R6247	T9392	T9389	conj	activity,mRNA
R6248	T9393	T9392	prep	of,activity
R6249	T9394	T9393	pobj	Cyp7a1,of
R6250	T9395	T9383	auxpass	were,decreased
R6251	T9396	T9383	punct	.,decreased
R6252	T9398	T9399	nsubjpass	Sitosterol,known
R6253	T9400	T9399	auxpass	is,known
R6254	T9401	T9402	aux	to,be
R6255	T9402	T9399	xcomp	be,known
R6256	T9403	T9404	det	a,inhibitor
R6257	T9404	T9402	attr	inhibitor,be
R6258	T9405	T9404	amod	direct,inhibitor
R6259	T9406	T9404	amod	competitive,inhibitor
R6260	T9407	T9404	prep	of,inhibitor
R6261	T9408	T9407	pobj	Cyp7a1,of
R6262	T9409	T9399	cc	and,known
R6263	T9410	T9411	nsubj	it,is
R6264	T9411	T9399	conj	is,known
R6265	T9412	T9411	acomp	likely,is
R6266	T9413	T9414	mark	that,are
R6267	T9414	T9411	ccomp	are,is
R6268	T9415	T9416	det	the,sterols
R6269	T9416	T9414	nsubj	sterols,are
R6270	T9417	T9416	amod	elevated,sterols
R6271	T9418	T9416	compound	plant,sterols
R6272	T9419	T9416	prep	in,sterols
R6273	T9420	T9421	det	the,liver
R6274	T9421	T9419	pobj	liver,in
R6275	T9422	T9414	acomp	responsible,are
R6276	T9423	T9422	prep	for,responsible
R6277	T9424	T9425	det	the,inhibition
R6278	T9425	T9423	pobj	inhibition,for
R6279	T9426	T9425	prep	in,inhibition
R6280	T9427	T9428	det	the,mouse
R6281	T9428	T9426	pobj	mouse,in
R6282	T9429	T9428	compound	knockout,mouse
R6283	T9430	T9431	punct	[,39
R6284	T9431	T9411	parataxis	39,is
R6285	T9432	T9431	punct	],39
R6286	T9433	T9411	punct	.,is
R6287	T9861	T9860	prep	of,Localization
R6288	T9862	T9863	compound	Abcg5,protein
R6289	T9863	T9861	pobj	protein,of
R6290	T9864	T9860	prep	in,Localization
R6291	T9865	T9866	nmod	Abcg8,knockout
R6292	T9866	T9870	compound	knockout,mice
R6293	T9867	T9865	punct	-,Abcg8
R6294	T9868	T9865	punct	/,Abcg8
R6295	T9869	T9865	punct	-,Abcg8
R6296	T9870	T9864	pobj	mice,in
R6297	T9872	T9873	aux	Does,result
R6298	T9874	T9875	det	the,loss
R6299	T9875	T9873	nsubj	loss,result
R6300	T9876	T9875	prep	of,loss
R6301	T9877	T9878	compound	Abcg8,sterolin
R6302	T9878	T9876	pobj	sterolin,of
R6303	T9879	T9878	punct	/,sterolin
R6304	T9880	T9878	punct	-,sterolin
R6305	T9881	T9878	nummod	2,sterolin
R6306	T9882	T9873	prep	in,result
R6307	T9883	T9882	pobj	loss,in
R6308	T9884	T9883	prep	of,loss
R6309	T9885	T9886	nmod	Abcg5,sterolin
R6310	T9886	T9888	nmod	sterolin,expression
R6311	T9887	T9886	punct	/,sterolin
R6312	T9888	T9884	pobj	expression,of
R6313	T9889	T9886	punct	-,sterolin
R6314	T9890	T9886	nummod	1,sterolin
R6315	T9891	T9873	punct	", ",result
R6316	T9892	T9893	mark	as,predicted
R6317	T9893	T9873	advcl	predicted,result
R6318	T9894	T9893	aux	might,predicted
R6319	T9895	T9893	auxpass	be,predicted
R6320	T9896	T9893	prep	from,predicted
R6321	T9897	T9898	det	the,studies
R6322	T9898	T9896	pobj	studies,from
R6323	T9899	T9898	amod	genetic,studies
R6324	T9900	T9896	cc	and,from
R6325	T9901	T9902	advmod	more,recently
R6326	T9902	T9903	advmod	recently,from
R6327	T9903	T9896	conj	from,from
R6328	T9904	T9905	det	the,studies
R6329	T9905	T9903	pobj	studies,from
R6330	T9906	T9907	advmod	in,vitro
R6331	T9907	T9905	amod	vitro,studies
R6332	T9908	T9907	cc	and,vitro
R6333	T9909	T9910	advmod	in,vivo
R6334	T9910	T9907	conj	vivo,vitro
R6335	T9911	T9905	compound	expression,studies
R6336	T9912	T9873	punct	?,result
R6337	T9914	T9915	det	A,antibody
R6338	T9915	T9917	nsubj	antibody,is
R6339	T9916	T9915	amod	robust,antibody
R6340	T9918	T9915	prep	to,antibody
R6341	T9919	T9920	compound	mouse,sterolin
R6342	T9920	T9918	pobj	sterolin,to
R6343	T9921	T9920	compound	Abcg8,sterolin
R6344	T9922	T9920	punct	/,sterolin
R6345	T9923	T9920	punct	-,sterolin
R6346	T9924	T9920	nummod	2,sterolin
R6347	T9925	T9917	neg	not,is
R6348	T9926	T9917	advmod	currently,is
R6349	T9927	T9917	acomp	available,is
R6350	T9928	T9917	punct	.,is
R6351	T9930	T9931	advmod	However,developed
R6352	T9932	T9931	punct	", ",developed
R6353	T9933	T9934	nummod	three,groups
R6354	T9934	T9931	nsubj	groups,developed
R6355	T9935	T9934	amod	separate,groups
R6356	T9936	T9931	aux	have,developed
R6357	T9937	T9938	nmod	rabbit,antibody
R6358	T9938	T9931	dobj	antibody,developed
R6359	T9939	T9938	amod	polyclonal,antibody
R6360	T9940	T9938	prep	to,antibody
R6361	T9941	T9942	compound	mouse,sterolin
R6362	T9942	T9940	pobj	sterolin,to
R6363	T9943	T9942	compound	Abcg5,sterolin
R6364	T9944	T9942	punct	/,sterolin
R6365	T9945	T9942	punct	-,sterolin
R6366	T9946	T9942	nummod	1,sterolin
R6367	T9947	T9931	punct	.,developed
R6368	T9949	T9950	det	These,antibodies
R6369	T9950	T9952	nsubjpass	antibodies,used
R6370	T9951	T9950	nummod	three,antibodies
R6371	T9953	T9952	auxpass	were,used
R6372	T9954	T9952	prep	in,used
R6373	T9955	T9956	compound	Western,blotting
R6374	T9956	T9954	pobj	blotting,in
R6375	T9957	T9956	cc	and,blotting
R6376	T9958	T9959	compound	immunohistochemistry,experiments
R6377	T9959	T9956	conj	experiments,blotting
R6378	T9960	T9952	prep	on,used
R6379	T9961	T9962	det	the,liver
R6380	T9962	T9960	pobj	liver,on
R6381	T9963	T9962	cc	and,liver
R6382	T9964	T9962	conj	intestine,liver
R6383	T9965	T9962	prep	of,liver
R6384	T9966	T9967	nmod	Abcg8,mice
R6385	T9967	T9965	pobj	mice,of
R6386	T9968	T9966	punct	-,Abcg8
R6387	T9969	T9966	punct	/,Abcg8
R6388	T9970	T9966	punct	-,Abcg8
R6389	T9971	T9952	punct	.,used
R6390	T9973	T9974	compound	Western,blotting
R6391	T9974	T9975	nsubj	blotting,showed
R6392	T9976	T9974	prep	of,blotting
R6393	T9977	T9978	nmod	liver,preparations
R6394	T9978	T9976	pobj	preparations,of
R6395	T9979	T9980	amod	total,membrane
R6396	T9980	T9978	compound	membrane,preparations
R6397	T9981	T9974	acl	using,blotting
R6398	T9982	T9983	det	the,antibodies
R6399	T9983	T9981	dobj	antibodies,using
R6400	T9984	T9983	nummod	three,antibodies
R6401	T9985	T9986	advmod	separately,developed
R6402	T9986	T9983	amod	developed,antibodies
R6403	T9987	T9986	punct	-,developed
R6404	T9988	T9989	compound	anti-Abcg5,sterolin
R6405	T9989	T9983	amod	sterolin,antibodies
R6406	T9990	T9989	punct	/,sterolin
R6407	T9991	T9989	punct	-,sterolin
R6408	T9992	T9989	nummod	1,sterolin
R6409	T9993	T9994	amod	different,results
R6410	T9994	T9975	dobj	results,showed
R6411	T9995	T9975	punct	.,showed
R6412	T9997	T9998	mark	As,published
R6413	T9998	T10002	advcl	published,exists
R6414	T9999	T9998	aux	has,published
R6415	T10000	T9998	auxpass	been,published
R6416	T10001	T9998	advmod	previously,published
R6417	T10003	T10002	punct	", ",exists
R6418	T10004	T10005	compound	Abcg5,sterolin
R6419	T10005	T10002	nsubj	sterolin,exists
R6420	T10006	T10005	punct	/,sterolin
R6421	T10007	T10005	punct	-,sterolin
R6422	T10008	T10005	nummod	1,sterolin
R6423	T10009	T10002	prep	in,exists
R6424	T10010	T10011	nummod	two,forms
R6425	T10011	T10009	pobj	forms,in
R6426	T10012	T10011	amod	separate,forms
R6427	T10013	T10011	punct	", ",forms
R6428	T10014	T10015	det	the,protein
R6429	T10015	T10011	appos	protein,forms
R6430	T10016	T10015	punct	',protein
R6431	T10017	T10015	nmod	immature,protein
R6432	T10018	T10015	punct	',protein
R6433	T10019	T10020	nummod	75,kDa
R6434	T10020	T10015	compound	kDa,protein
R6435	T10021	T10015	cc	and,protein
R6436	T10022	T10023	det	a,protein
R6437	T10023	T10015	conj	protein,protein
R6438	T10024	T10025	advmod	fully,glycosylated
R6439	T10025	T10023	amod	glycosylated,protein
R6440	T10026	T10023	punct	',protein
R6441	T10027	T10023	nmod	mature,protein
R6442	T10028	T10023	punct	',protein
R6443	T10029	T10030	nummod	93,kDa
R6444	T10030	T10023	compound	kDa,protein
R6445	T10031	T10032	punct	[,30
R6446	T10032	T10002	parataxis	30,exists
R6447	T10033	T10032	nummod	18,30
R6448	T10034	T10032	punct	",",30
R6449	T10035	T10032	punct	],30
R6450	T10036	T10002	punct	.,exists
R6451	T10038	T10039	advcl	Using,detected
R6452	T10040	T10041	det	the,antibody
R6453	T10041	T10038	dobj	antibody,Using
R6454	T10042	T10043	nmod	SC,peptide
R6455	T10043	T10041	compound	peptide,antibody
R6456	T10044	T10045	compound	anti-Abcg5,sterolin
R6457	T10045	T10043	amod	sterolin,peptide
R6458	T10046	T10045	punct	/,sterolin
R6459	T10047	T10045	punct	-,sterolin
R6460	T10048	T10045	nummod	1,sterolin
R6461	T10049	T10039	punct	", ",detected
R6462	T10050	T10051	det	a,band
R6463	T10051	T10039	nsubjpass	band,detected
R6464	T10052	T10053	nummod	75,kDa
R6465	T10053	T10051	compound	kDa,band
R6466	T10054	T10039	auxpass	is,detected
R6467	T10055	T10056	punct	(,5a
R6468	T10056	T10039	parataxis	5a,detected
R6469	T10057	T10056	compound	Figure,5a
R6470	T10058	T10056	punct	),5a
R6471	T10059	T10039	punct	.,detected
R6472	T10061	T10062	det	This,antibody
R6473	T10062	T10063	nsubj	antibody,detect
R6474	T10064	T10063	aux	does,detect
R6475	T10065	T10063	neg	not,detect
R6476	T10066	T10067	det	a,band
R6477	T10067	T10063	dobj	band,detect
R6478	T10068	T10067	punct	',band
R6479	T10069	T10067	amod	mature,band
R6480	T10070	T10067	punct	',band
R6481	T10071	T10072	nummod	93,kDa
R6482	T10072	T10067	compound	kDa,band
R6483	T10073	T10063	prep	in,detect
R6484	T10074	T10075	preconj	either,animals
R6485	T10075	T10073	pobj	animals,in
R6486	T10076	T10077	amod	wild,type
R6487	T10077	T10075	nmod	type,animals
R6488	T10078	T10077	punct	-,type
R6489	T10079	T10077	cc	or,type
R6490	T10080	T10077	conj	knockout,type
R6491	T10081	T10063	cc	nor,detect
R6492	T10082	T10063	conj	is,detect
R6493	T10083	T10084	det	the,band
R6494	T10084	T10082	nsubj	band,is
R6495	T10085	T10086	nummod	75,kDa
R6496	T10086	T10084	compound	kDa,band
R6497	T10087	T10082	acomp	sensitive,is
R6498	T10088	T10087	prep	to,sensitive
R6499	T10089	T10090	preconj	either,H
R6500	T10090	T10088	pobj	H,to
R6501	T10091	T10090	compound	Endo,H
R6502	T10092	T10090	punct	-,H
R6503	T10093	T10090	cc	or,H
R6504	T10094	T10090	conj	PNGaseF,H
R6505	T10095	T10096	punct	(,shows
R6506	T10096	T10082	parataxis	shows,is
R6507	T10097	T10098	amod	lower,panel
R6508	T10098	T10096	nsubj	panel,shows
R6509	T10099	T10100	amod	same,aliquots
R6510	T10100	T10096	dobj	aliquots,shows
R6511	T10101	T10100	acl	probed,aliquots
R6512	T10102	T10101	prep	with,probed
R6513	T10103	T10102	pobj	anti-transferrin,with
R6514	T10104	T10096	punct	),shows
R6515	T10105	T10082	punct	.,is
R6516	T10107	T10108	det	The,antibody
R6517	T10108	T10110	nsubj	antibody,detects
R6518	T10109	T10108	compound	AMC,antibody
R6519	T10111	T10112	det	the,form
R6520	T10112	T10110	dobj	form,detects
R6521	T10113	T10112	punct	',form
R6522	T10114	T10112	nmod	mature,form
R6523	T10115	T10112	punct	',form
R6524	T10116	T10112	prep	of,form
R6525	T10117	T10118	compound	Abcg5,sterolin
R6526	T10118	T10116	pobj	sterolin,of
R6527	T10119	T10118	punct	/,sterolin
R6528	T10120	T10118	punct	-,sterolin
R6529	T10121	T10118	nummod	1,sterolin
R6530	T10122	T10112	prep	in,form
R6531	T10123	T10124	amod	wild,type
R6532	T10124	T10126	compound	type,animals
R6533	T10125	T10124	punct	-,type
R6534	T10126	T10122	pobj	animals,in
R6535	T10127	T10126	cc	but,animals
R6536	T10128	T10127	neg	not,but
R6537	T10129	T10130	det	the,knockouts
R6538	T10130	T10126	conj	knockouts,animals
R6539	T10131	T10110	punct	.,detects
R6540	T10133	T10134	advmod	Interestingly,detected
R6541	T10135	T10134	punct	", ",detected
R6542	T10136	T10137	det	the,band
R6543	T10137	T10134	nsubjpass	band,detected
R6544	T10138	T10137	punct	',band
R6545	T10139	T10137	amod	immature,band
R6546	T10140	T10137	punct	',band
R6547	T10141	T10142	nummod	75,kDa
R6548	T10142	T10137	compound	kDa,band
R6549	T10143	T10134	auxpass	is,detected
R6550	T10144	T10134	prep	by,detected
R6551	T10145	T10146	det	this,antibody
R6552	T10146	T10144	pobj	antibody,by
R6553	T10147	T10134	cc	but,detected
R6554	T10148	T10149	det	the,band
R6555	T10149	T10150	nsubj	band,shifts
R6556	T10150	T10134	conj	shifts,detected
R6557	T10151	T10150	prep	with,shifts
R6558	T10152	T10153	nmod	Endo,H
R6559	T10153	T10155	nmod	H,treatment
R6560	T10154	T10153	punct	-,H
R6561	T10155	T10151	pobj	treatment,with
R6562	T10156	T10153	cc	and,H
R6563	T10157	T10158	compound	PNGase,F
R6564	T10158	T10153	conj	F,H
R6565	T10159	T10160	punct	(,5b
R6566	T10160	T10150	parataxis	5b,shifts
R6567	T10161	T10160	compound	Figure,5b
R6568	T10162	T10160	punct	),5b
R6569	T10163	T10150	punct	.,shifts
R6570	T10165	T10166	det	The,antibody
R6571	T10166	T10168	nsubj	antibody,detects
R6572	T10167	T10166	compound	UTSW,antibody
R6573	T10169	T10170	det	the,forms
R6574	T10170	T10168	dobj	forms,detects
R6575	T10171	T10170	punct	',forms
R6576	T10172	T10170	amod	mature,forms
R6577	T10173	T10172	punct	',mature
R6578	T10174	T10172	cc	and,mature
R6579	T10175	T10176	punct	',immature
R6580	T10176	T10172	conj	immature,mature
R6581	T10177	T10170	punct	',forms
R6582	T10178	T10170	prep	of,forms
R6583	T10179	T10180	compound	Abcg5,sterolin
R6584	T10180	T10178	pobj	sterolin,of
R6585	T10181	T10180	punct	/,sterolin
R6586	T10182	T10180	punct	-,sterolin
R6587	T10183	T10180	nummod	1,sterolin
R6588	T10184	T10168	prep	in,detects
R6589	T10185	T10186	amod	wild,type
R6590	T10186	T10188	compound	type,animals
R6591	T10187	T10186	punct	-,type
R6592	T10188	T10184	pobj	animals,in
R6593	T10189	T10168	cc	but,detects
R6594	T10190	T10168	conj	detects,detects
R6595	T10191	T10192	det	no,forms
R6596	T10192	T10190	dobj	forms,detects
R6597	T10193	T10190	prep	in,detects
R6598	T10194	T10195	det	the,mouse
R6599	T10195	T10193	pobj	mouse,in
R6600	T10196	T10195	compound	knockout,mouse
R6601	T10197	T10198	punct	(,5c
R6602	T10198	T10190	parataxis	5c,detects
R6603	T10199	T10198	compound	Figure,5c
R6604	T10200	T10198	punct	),5c
R6605	T10201	T10168	punct	.,detects
R6606	T10203	T10204	dep	What,hold
R6607	T10204	T10206	csubj	hold,is
R6608	T10205	T10204	aux	does,hold
R6609	T10207	T10204	acomp	true,hold
R6610	T10208	T10204	prep	for,hold
R6611	T10209	T10210	det	all,antibodies
R6612	T10210	T10208	pobj	antibodies,for
R6613	T10211	T10210	acl	used,antibodies
R6614	T10212	T10211	prep	against,used
R6615	T10213	T10214	compound	Abcg5,sterolin
R6616	T10214	T10212	pobj	sterolin,against
R6617	T10215	T10214	punct	/,sterolin
R6618	T10216	T10214	punct	-,sterolin
R6619	T10217	T10214	nummod	1,sterolin
R6620	T10218	T10219	det	the,detection
R6621	T10219	T10206	attr	detection,is
R6622	T10220	T10219	prep	of,detection
R6623	T10221	T10222	det	a,band
R6624	T10222	T10220	pobj	band,of
R6625	T10223	T10224	nummod	75,kDa
R6626	T10224	T10222	compound	kDa,band
R6627	T10225	T10206	punct	.,is
R6628	T10227	T10228	compound	Immunohistochemistry,experiments
R6629	T10228	T10229	nsubjpass	experiments,carried
R6630	T10230	T10229	auxpass	were,carried
R6631	T10231	T10229	prt	out,carried
R6632	T10232	T10229	advcl	using,carried
R6633	T10233	T10234	det	all,antibodies
R6634	T10234	T10232	dobj	antibodies,using
R6635	T10235	T10234	nummod	three,antibodies
R6636	T10236	T10229	punct	.,carried
R6637	T10238	T10239	prep	In,recognized
R6638	T10240	T10241	amod	acute,studies
R6639	T10241	T10238	pobj	studies,In
R6640	T10242	T10241	compound	transfection,studies
R6641	T10243	T10239	punct	", ",recognized
R6642	T10244	T10245	det	the,antibody
R6643	T10245	T10239	nsubj	antibody,recognized
R6644	T10246	T10245	compound	SC,antibody
R6645	T10247	T10248	amod	over-expressed,sterolin
R6646	T10248	T10239	dobj	sterolin,recognized
R6647	T10249	T10248	compound	mouse,sterolin
R6648	T10250	T10248	compound	Abcg5,sterolin
R6649	T10251	T10248	punct	/,sterolin
R6650	T10252	T10248	punct	-,sterolin
R6651	T10253	T10248	nummod	1,sterolin
R6652	T10254	T10248	prep	in,sterolin
R6653	T10255	T10256	nmod	COS,cells
R6654	T10256	T10254	pobj	cells,in
R6655	T10257	T10255	punct	-,COS
R6656	T10258	T10255	nummod	1,COS
R6657	T10259	T10248	punct	", ",sterolin
R6658	T10260	T10248	cc	but,sterolin
R6659	T10261	T10260	neg	not,but
R6660	T10262	T10263	compound	mouse,sterolin
R6661	T10263	T10248	conj	sterolin,sterolin
R6662	T10264	T10263	compound	Abcg8,sterolin
R6663	T10265	T10263	punct	/,sterolin
R6664	T10266	T10263	punct	-,sterolin
R6665	T10267	T10263	nummod	2,sterolin
R6666	T10268	T10269	punct	(,6c
R6667	T10269	T10239	parataxis	6c,recognized
R6668	T10270	T10269	nmod	Figure,6c
R6669	T10271	T10269	nmod	6b,6c
R6670	T10272	T10269	punct	",",6c
R6671	T10273	T10269	punct	),6c
R6672	T10274	T10239	punct	.,recognized
R6673	T10276	T10277	nsubj	Co-transfection,alter
R6674	T10278	T10276	prep	of,Co-transfection
R6675	T10279	T10280	nmod	COS,cells
R6676	T10280	T10278	pobj	cells,of
R6677	T10281	T10279	punct	-,COS
R6678	T10282	T10279	nummod	1,COS
R6679	T10283	T10276	prep	with,Co-transfection
R6680	T10284	T10285	preconj	both,Abcg5
R6681	T10285	T10286	nmod	Abcg5,cDNAs
R6682	T10286	T10283	pobj	cDNAs,with
R6683	T10287	T10285	cc	and,Abcg5
R6684	T10288	T10285	conj	Abcg8,Abcg5
R6685	T10289	T10277	aux	did,alter
R6686	T10290	T10277	neg	not,alter
R6687	T10291	T10292	det	the,pattern
R6688	T10292	T10277	dobj	pattern,alter
R6689	T10293	T10292	prep	of,pattern
R6690	T10294	T10293	pobj	immunofluorescence,of
R6691	T10295	T10277	punct	", ",alter
R6692	T10296	T10297	mark	although,are
R6693	T10297	T10277	advcl	are,alter
R6694	T10298	T10297	nsubj	we,are
R6695	T10299	T10297	neg	not,are
R6696	T10300	T10297	acomp	able,are
R6697	T10301	T10302	aux	to,confirm
R6698	T10302	T10300	xcomp	confirm,able
R6699	T10303	T10304	amod	simultaneous,expression
R6700	T10304	T10302	dobj	expression,confirm
R6701	T10305	T10304	prep	of,expression
R6702	T10306	T10305	pobj	Abcg8,of
R6703	T10307	T10304	prep	in,expression
R6704	T10308	T10307	pobj	cells,in
R6705	T10309	T10308	acl	expressing,cells
R6706	T10310	T10311	compound	Abcg5,sterolin
R6707	T10311	T10309	dobj	sterolin,expressing
R6708	T10312	T10311	punct	/,sterolin
R6709	T10313	T10311	punct	-,sterolin
R6710	T10314	T10311	nummod	1,sterolin
R6711	T10315	T10297	punct	", ",are
R6712	T10316	T10317	mark	as,have
R6713	T10317	T10297	advcl	have,are
R6714	T10318	T10317	nsubj	we,have
R6715	T10319	T10320	det	no,antibody
R6716	T10320	T10317	dobj	antibody,have
R6717	T10321	T10320	amod	suitable,antibody
R6718	T10322	T10320	prep	for,antibody
R6719	T10323	T10324	compound	Abcg8,sterolin
R6720	T10324	T10322	pobj	sterolin,for
R6721	T10325	T10324	punct	/,sterolin
R6722	T10326	T10324	punct	-,sterolin
R6723	T10327	T10324	nummod	2,sterolin
R6724	T10328	T10329	punct	(,6d
R6725	T10329	T10277	parataxis	6d,alter
R6726	T10330	T10329	compound	Figure,6d
R6727	T10331	T10329	punct	),6d
R6728	T10332	T10277	punct	.,alter
R6729	T10334	T10335	advmod	Nevertheless,indicates
R6730	T10336	T10335	punct	", ",indicates
R6731	T10337	T10338	det	this,experiment
R6732	T10338	T10335	nsubj	experiment,indicates
R6733	T10339	T10340	mark	that,recognize
R6734	T10340	T10335	ccomp	recognize,indicates
R6735	T10341	T10342	det	the,antibody
R6736	T10342	T10340	nsubj	antibody,recognize
R6737	T10343	T10342	compound	SC,antibody
R6738	T10344	T10340	aux	can,recognize
R6739	T10345	T10346	compound	Abcg5,sterolin
R6740	T10346	T10340	dobj	sterolin,recognize
R6741	T10347	T10346	punct	/,sterolin
R6742	T10348	T10346	punct	-,sterolin
R6743	T10349	T10346	nummod	1,sterolin
R6744	T10350	T10340	cc	but,recognize
R6745	T10351	T10352	aux	does,cross-react
R6746	T10352	T10340	conj	cross-react,recognize
R6747	T10353	T10352	neg	not,cross-react
R6748	T10354	T10352	prep	with,cross-react
R6749	T10355	T10356	compound	Abcg8,sterolin
R6750	T10356	T10354	pobj	sterolin,with
R6751	T10357	T10356	punct	/,sterolin
R6752	T10358	T10356	punct	-,sterolin
R6753	T10359	T10356	nummod	2,sterolin
R6754	T10360	T10335	punct	.,indicates
R6755	T10362	T10363	amod	Serial,sections
R6756	T10363	T10364	nsubjpass	sections,incubated
R6757	T10365	T10363	prep	of,sections
R6758	T10366	T10367	amod	intestinal,tissue
R6759	T10367	T10365	pobj	tissue,of
R6760	T10368	T10364	auxpass	were,incubated
R6761	T10369	T10364	prep	with,incubated
R6762	T10370	T10371	compound	anti-Abcg5,sterolin
R6763	T10371	T10369	pobj	sterolin,with
R6764	T10372	T10371	punct	/,sterolin
R6765	T10373	T10371	punct	-,sterolin
R6766	T10374	T10371	nummod	1,sterolin
R6767	T10375	T10376	aux	to,determine
R6768	T10376	T10364	advcl	determine,incubated
R6769	T10377	T10378	det	the,location
R6770	T10378	T10376	dobj	location,determine
R6771	T10379	T10378	amod	cellular,location
R6772	T10380	T10378	prep	of,location
R6773	T10381	T10382	compound	Abcg5,sterolin
R6774	T10382	T10380	pobj	sterolin,of
R6775	T10383	T10382	punct	/,sterolin
R6776	T10384	T10382	punct	-,sterolin
R6777	T10385	T10382	nummod	1,sterolin
R6778	T10386	T10364	punct	.,incubated
R6779	T10388	T10389	det	The,pattern
R6780	T10389	T10390	nsubj	pattern,is
R6781	T10391	T10389	prep	of,pattern
R6782	T10392	T10391	pobj	staining,of
R6783	T10393	T10389	prep	of,pattern
R6784	T10394	T10395	compound	Abcg5,sterolin
R6785	T10395	T10393	pobj	sterolin,of
R6786	T10396	T10395	punct	/,sterolin
R6787	T10397	T10395	punct	-,sterolin
R6788	T10398	T10395	nummod	1,sterolin
R6789	T10399	T10389	prep	in,pattern
R6790	T10400	T10401	det	the,sections
R6791	T10401	T10399	pobj	sections,in
R6792	T10402	T10401	amod	intestinal,sections
R6793	T10403	T10404	advmod	clearly,apical
R6794	T10404	T10390	acomp	apical,is
R6795	T10405	T10404	cc	and,apical
R6796	T10406	T10404	conj	localized,apical
R6797	T10407	T10406	prep	to,localized
R6798	T10408	T10409	det	the,villi
R6799	T10409	T10407	pobj	villi,to
R6800	T10410	T10409	prep	of,villi
R6801	T10411	T10412	det	the,enterocytes
R6802	T10412	T10410	pobj	enterocytes,of
R6803	T10413	T10414	punct	(,6i
R6804	T10414	T10390	parataxis	6i,is
R6805	T10415	T10414	nmod	Figure,6i
R6806	T10416	T10414	nmod	6g,6i
R6807	T10417	T10414	punct	",",6i
R6808	T10418	T10414	nmod	6h,6i
R6809	T10419	T10414	punct	",",6i
R6810	T10420	T10414	punct	),6i
R6811	T10421	T10390	punct	.,is
R6812	T10423	T10424	nsubj	Loss,seem
R6813	T10425	T10423	prep	of,Loss
R6814	T10426	T10427	compound	Abcg8,sterolin
R6815	T10427	T10425	pobj	sterolin,of
R6816	T10428	T10427	punct	/,sterolin
R6817	T10429	T10427	punct	-,sterolin
R6818	T10430	T10427	nummod	2,sterolin
R6819	T10431	T10424	aux	does,seem
R6820	T10432	T10424	neg	not,seem
R6821	T10433	T10434	aux	to,affect
R6822	T10434	T10424	xcomp	affect,seem
R6823	T10435	T10436	det	this,pattern
R6824	T10436	T10434	dobj	pattern,affect
R6825	T10437	T10436	prep	of,pattern
R6826	T10438	T10437	pobj	staining,of
R6827	T10439	T10424	punct	.,seem
R6828	T10441	T10442	aux	To,confirm
R6829	T10442	T10444	advcl	confirm,blocked
R6830	T10443	T10442	advmod	further,confirm
R6831	T10445	T10446	mark	that,recognizes
R6832	T10446	T10442	ccomp	recognizes,confirm
R6833	T10447	T10448	det	the,antibody
R6834	T10448	T10446	nsubj	antibody,recognizes
R6835	T10449	T10448	acl	used,antibody
R6836	T10450	T10451	compound	Abcg5,sterolin
R6837	T10451	T10446	dobj	sterolin,recognizes
R6838	T10452	T10451	punct	/,sterolin
R6839	T10453	T10451	punct	-,sterolin
R6840	T10454	T10451	nummod	1,sterolin
R6841	T10455	T10444	punct	;,blocked
R6842	T10456	T10457	det	this,pattern
R6843	T10457	T10444	nsubjpass	pattern,blocked
R6844	T10458	T10444	auxpass	is,blocked
R6845	T10459	T10460	mark	if,pre-incubated
R6846	T10460	T10444	advcl	pre-incubated,blocked
R6847	T10461	T10462	det	the,antibody
R6848	T10462	T10460	nsubjpass	antibody,pre-incubated
R6849	T10463	T10460	auxpass	is,pre-incubated
R6850	T10464	T10460	prep	with,pre-incubated
R6851	T10465	T10466	det	the,peptide
R6852	T10466	T10464	pobj	peptide,with
R6853	T10467	T10468	prep	to,raised
R6854	T10468	T10466	relcl	raised,peptide
R6855	T10469	T10467	pobj	which,to
R6856	T10470	T10468	nsubjpass	it,raised
R6857	T10471	T10468	auxpass	was,raised
R6858	T10472	T10473	punct	(,6f
R6859	T10473	T10444	parataxis	6f,blocked
R6860	T10474	T10473	compound	Figure,6f
R6861	T10475	T10473	punct	),6f
R6862	T10476	T10444	punct	.,blocked
R6863	T10478	T10479	aux	To,confirm
R6864	T10479	T10481	advcl	confirm,performed
R6865	T10480	T10479	advmod	further,confirm
R6866	T10482	T10483	mark	that,preserved
R6867	T10483	T10479	ccomp	preserved,confirm
R6868	T10484	T10485	nmod	Abcg5,sterolin
R6869	T10485	T10487	nmod	sterolin,expression
R6870	T10486	T10485	punct	/,sterolin
R6871	T10487	T10483	nsubjpass	expression,preserved
R6872	T10488	T10485	punct	-,sterolin
R6873	T10489	T10485	nummod	1,sterolin
R6874	T10490	T10483	auxpass	is,preserved
R6875	T10491	T10483	cc	and,preserved
R6876	T10492	T10493	aux	may,be
R6877	T10493	T10483	conj	be,preserved
R6878	T10494	T10493	acomp	apical,be
R6879	T10495	T10481	punct	", ",performed
R6880	T10496	T10481	nsubjpass	immunohistochemistry,performed
R6881	T10497	T10481	auxpass	was,performed
R6882	T10498	T10481	prep	in,performed
R6883	T10499	T10498	pobj	Amsterdam,in
R6884	T10500	T10481	advcl	using,performed
R6885	T10501	T10502	det	the,antibody
R6886	T10502	T10500	dobj	antibody,using
R6887	T10503	T10502	compound	AMC,antibody
R6888	T10504	T10505	aux	to,stain
R6889	T10505	T10500	advcl	stain,using
R6890	T10506	T10507	compound	liver,sections
R6891	T10507	T10505	dobj	sections,stain
R6892	T10508	T10507	prep	from,sections
R6893	T10509	T10510	amod	wild,type
R6894	T10510	T10512	nmod	type,mice
R6895	T10511	T10510	punct	-,type
R6896	T10512	T10508	pobj	mice,from
R6897	T10513	T10510	cc	and,type
R6898	T10514	T10510	conj	knockout,type
R6899	T10515	T10516	punct	[,30
R6900	T10516	T10481	parataxis	30,performed
R6901	T10517	T10516	punct	],30
R6902	T10518	T10481	punct	.,performed
R6903	T10520	T10521	prep	As,compared
R6904	T10522	T10523	det	a,control
R6905	T10523	T10520	pobj	control,As
R6906	T10524	T10521	punct	", ",compared
R6907	T10525	T10526	det	the,expression
R6908	T10526	T10521	nsubjpass	expression,compared
R6909	T10527	T10526	prep	of,expression
R6910	T10528	T10527	pobj	Abcb11,of
R6911	T10529	T10528	punct	", ",Abcb11
R6912	T10530	T10531	det	an,transporter
R6913	T10531	T10528	appos	transporter,Abcb11
R6914	T10532	T10531	compound	ABC,transporter
R6915	T10533	T10531	acl	known,transporter
R6916	T10534	T10535	aux	to,be
R6917	T10535	T10533	xcomp	be,known
R6918	T10536	T10535	acomp	responsible,be
R6919	T10537	T10536	prep	for,responsible
R6920	T10538	T10539	det	the,export
R6921	T10539	T10537	pobj	export,for
R6922	T10540	T10539	prep	of,export
R6923	T10541	T10542	compound	bile,salts
R6924	T10542	T10540	pobj	salts,of
R6925	T10543	T10521	punct	", ",compared
R6926	T10544	T10521	auxpass	was,compared
R6927	T10545	T10521	prep	to,compared
R6928	T10546	T10545	pobj	that,to
R6929	T10547	T10546	prep	of,that
R6930	T10548	T10549	compound	Abcg5,sterolin
R6931	T10549	T10547	pobj	sterolin,of
R6932	T10550	T10549	punct	/,sterolin
R6933	T10551	T10549	punct	-,sterolin
R6934	T10552	T10549	nummod	1,sterolin
R6935	T10553	T10554	punct	(,6k
R6936	T10554	T10521	parataxis	6k,compared
R6937	T10555	T10554	nmod	Figure,6k
R6938	T10556	T10554	nmod	6j,6k
R6939	T10557	T10554	punct	",",6k
R6940	T10558	T10554	punct	),6k
R6941	T10559	T10521	punct	.,compared
R6942	T10561	T10562	advmod	Again,localized
R6943	T10563	T10562	punct	", ",localized
R6944	T10564	T10565	det	both,proteins
R6945	T10565	T10562	nsubjpass	proteins,localized
R6946	T10566	T10562	auxpass	are,localized
R6947	T10567	T10562	advmod	apically,localized
R6948	T10568	T10562	prep	in,localized
R6949	T10569	T10570	compound	liver,sections
R6950	T10570	T10568	pobj	sections,in
R6951	T10571	T10570	prep	from,sections
R6952	T10572	T10573	amod	wild,type
R6953	T10573	T10575	compound	type,mice
R6954	T10574	T10573	punct	-,type
R6955	T10575	T10571	pobj	mice,from
R6956	T10576	T10562	cc	and,localized
R6957	T10577	T10578	det	this,pattern
R6958	T10578	T10579	nsubj	pattern,is
R6959	T10579	T10562	conj	is,localized
R6960	T10580	T10581	advmod	essentially,unperturbed
R6961	T10581	T10579	acomp	unperturbed,is
R6962	T10582	T10579	prep	in,is
R6963	T10583	T10582	pobj	liver,in
R6964	T10584	T10583	prep	from,liver
R6965	T10585	T10586	compound	knockout,mice
R6966	T10586	T10584	pobj	mice,from
R6967	T10587	T10579	punct	", ",is
R6968	T10588	T10589	mark	although,seems
R6969	T10589	T10579	advcl	seems,is
R6970	T10590	T10591	det	the,level
R6971	T10591	T10589	nsubj	level,seems
R6972	T10592	T10591	compound	expression,level
R6973	T10593	T10591	prep	of,level
R6974	T10594	T10595	compound	Abcg5,sterolin
R6975	T10595	T10593	pobj	sterolin,of
R6976	T10596	T10595	punct	/,sterolin
R6977	T10597	T10595	punct	-,sterolin
R6978	T10598	T10595	nummod	1,sterolin
R6979	T10599	T10600	aux	to,be
R6980	T10600	T10589	xcomp	be,seems
R6981	T10601	T10602	advmod	less,robust
R6982	T10602	T10600	acomp	robust,be
R6983	T10603	T10600	advmod	qualitatively,be
R6984	T10604	T10605	punct	(,6m
R6985	T10605	T10579	parataxis	6m,is
R6986	T10606	T10605	nmod	Figure,6m
R6987	T10607	T10605	nmod	6l,6m
R6988	T10608	T10605	punct	",",6m
R6989	T10609	T10605	punct	),6m
R6990	T10610	T10579	punct	.,is
R6991	T10612	T10613	aux	To,confirm
R6992	T10613	T10615	advcl	confirm,used
R6993	T10614	T10613	advmod	further,confirm
R6994	T10616	T10617	det	the,expression
R6995	T10617	T10613	dobj	expression,confirm
R6996	T10618	T10617	amod	apical,expression
R6997	T10619	T10617	prep	of,expression
R6998	T10620	T10621	compound	Abcg5,sterolin
R6999	T10621	T10619	pobj	sterolin,of
R7000	T10622	T10621	punct	/,sterolin
R7001	T10623	T10621	punct	-,sterolin
R7002	T10624	T10621	nummod	1,sterolin
R7003	T10625	T10617	prep	in,expression
R7004	T10626	T10627	det	the,mice
R7005	T10627	T10625	pobj	mice,in
R7006	T10628	T10627	compound	knockout,mice
R7007	T10629	T10615	punct	", ",used
R7008	T10630	T10631	det	the,antibody
R7009	T10631	T10615	nsubjpass	antibody,used
R7010	T10632	T10631	compound	UTSW,antibody
R7011	T10633	T10615	auxpass	was,used
R7012	T10634	T10635	aux	to,stain
R7013	T10635	T10615	advcl	stain,used
R7014	T10636	T10637	compound	liver,sections
R7015	T10637	T10635	dobj	sections,stain
R7016	T10638	T10637	prep	of,sections
R7017	T10639	T10640	amod	wild,type
R7018	T10640	T10642	nmod	type,mice
R7019	T10641	T10640	punct	-,type
R7020	T10642	T10638	pobj	mice,of
R7021	T10643	T10640	cc	and,type
R7022	T10644	T10640	conj	knockout,type
R7023	T10645	T10615	punct	.,used
R7024	T10647	T10648	advmod	Again,is
R7025	T10649	T10648	punct	", ",is
R7026	T10650	T10648	expl	there,is
R7027	T10651	T10652	amod	apical,expression
R7028	T10652	T10648	attr	expression,is
R7029	T10653	T10652	prep	of,expression
R7030	T10654	T10655	compound	Abcg5,sterolin
R7031	T10655	T10653	pobj	sterolin,of
R7032	T10656	T10655	punct	/,sterolin
R7033	T10657	T10655	punct	-,sterolin
R7034	T10658	T10655	nummod	1,sterolin
R7035	T10659	T10648	prep	in,is
R7036	T10660	T10661	preconj	both,type
R7037	T10661	T10664	nmod	type,livers
R7038	T10662	T10661	amod	wild,type
R7039	T10663	T10661	punct	-,type
R7040	T10664	T10659	pobj	livers,in
R7041	T10665	T10661	cc	and,type
R7042	T10666	T10661	conj	knockout,type
R7043	T10667	T10668	punct	(,7b
R7044	T10668	T10648	parataxis	7b,is
R7045	T10669	T10668	nmod	Figure,7b
R7046	T10670	T10668	nmod	7a,7b
R7047	T10671	T10668	punct	",",7b
R7048	T10672	T10668	punct	),7b
R7049	T10673	T10648	punct	.,is
R7050	T10961	T10962	amod	Biliary,secretion
R7051	T10963	T10962	prep	of,secretion
R7052	T10964	T10965	compound	bile,salts
R7053	T10965	T10963	pobj	salts,of
R7054	T10966	T10965	punct	", ",salts
R7055	T10967	T10965	conj	sterol,salts
R7056	T10968	T10967	cc	and,sterol
R7057	T10969	T10967	conj	phospholipids,sterol
R7058	T10970	T10962	prep	in,secretion
R7059	T10971	T10972	compound	Abcg8,sterolin
R7060	T10972	T10974	npadvmod	sterolin,deficient
R7061	T10973	T10972	punct	/,sterolin
R7062	T10974	T10977	amod	deficient,mice
R7063	T10975	T10972	punct	-,sterolin
R7064	T10976	T10972	nummod	2,sterolin
R7065	T10977	T10970	pobj	mice,in
R7066	T10979	T10980	amod	Sitosterolemic,individuals
R7067	T10980	T10981	nsubj	individuals,have
R7068	T10982	T10983	det	an,ability
R7069	T10983	T10981	dobj	ability,have
R7070	T10984	T10983	amod	impaired,ability
R7071	T10985	T10986	aux	to,secrete
R7072	T10986	T10983	acl	secrete,ability
R7073	T10987	T10986	dobj	cholesterol,secrete
R7074	T10988	T10987	cc	and,cholesterol
R7075	T10989	T10990	compound	plant,sterols
R7076	T10990	T10987	conj	sterols,cholesterol
R7077	T10991	T10986	prep	into,secrete
R7078	T10992	T10991	pobj	bile,into
R7079	T10993	T10994	punct	[,41
R7080	T10994	T10981	parataxis	41,have
R7081	T10995	T10994	nummod	40,41
R7082	T10996	T10994	punct	",",41
R7083	T10997	T10994	punct	],41
R7084	T10998	T10981	punct	.,have
R7085	T11000	T11001	nsubj	We,analyzed
R7086	T11002	T11003	det	the,mice
R7087	T11003	T11001	dobj	mice,analyzed
R7088	T11004	T11003	compound	knockout,mice
R7089	T11005	T11001	prep	for,analyzed
R7090	T11006	T11007	det	any,alterations
R7091	T11007	T11005	pobj	alterations,for
R7092	T11008	T11007	prep	in,alterations
R7093	T11009	T11010	amod	biliary,sterol
R7094	T11010	T11011	compound	sterol,handling
R7095	T11011	T11008	pobj	handling,in
R7096	T11012	T11001	punct	.,analyzed
R7097	T11014	T11015	nsubj	Analyses,showed
R7098	T11016	T11014	prep	of,Analyses
R7099	T11017	T11018	det	the,secretion
R7100	T11018	T11016	pobj	secretion,of
R7101	T11019	T11018	amod	initial,secretion
R7102	T11020	T11018	amod	basal,secretion
R7103	T11021	T11018	compound	bile,secretion
R7104	T11022	T11023	mark	that,reduced
R7105	T11023	T11015	ccomp	reduced,showed
R7106	T11024	T11025	det	the,contents
R7107	T11025	T11023	nsubjpass	contents,reduced
R7108	T11026	T11025	nmod	sterol,contents
R7109	T11027	T11026	cc	and,sterol
R7110	T11028	T11026	conj	phospholipid,sterol
R7111	T11029	T11025	prep	in,contents
R7112	T11030	T11031	nmod	Abcg8,mice
R7113	T11031	T11029	pobj	mice,in
R7114	T11032	T11030	punct	-,Abcg8
R7115	T11033	T11030	punct	/,Abcg8
R7116	T11034	T11030	punct	-,Abcg8
R7117	T11035	T11023	auxpass	were,reduced
R7118	T11036	T11037	mark	as,compared
R7119	T11037	T11023	advcl	compared,reduced
R7120	T11038	T11037	prep	to,compared
R7121	T11039	T11040	amod	wild,type
R7122	T11040	T11042	compound	type,mice
R7123	T11041	T11040	punct	-,type
R7124	T11042	T11038	pobj	mice,to
R7125	T11043	T11015	punct	", ",showed
R7126	T11044	T11045	punct	(,8c
R7127	T11045	T11015	parataxis	8c,showed
R7128	T11046	T11045	nmod	Figure,8c
R7129	T11047	T11045	nmod	8a,8c
R7130	T11048	T11045	punct	",",8c
R7131	T11049	T11045	nmod	8b,8c
R7132	T11050	T11045	punct	",",8c
R7133	T11051	T11045	punct	", ",8c
R7134	T11052	T11053	compound	bile,salt
R7135	T11053	T11054	compound	salt,secretion
R7136	T11054	T11055	nsubj	secretion,significant
R7137	T11055	T11058	amod	significant,0.01
R7138	T11056	T11055	neg	not,significant
R7139	T11057	T11055	advmod	statistically,significant
R7140	T11058	T11045	ccomp	0.01,8c
R7141	T11059	T11058	punct	", ",0.01
R7142	T11060	T11058	prep	for,0.01
R7143	T11061	T11062	compound	sterol,secretion
R7144	T11062	T11060	pobj	secretion,for
R7145	T11063	T11058	nsubj	P,0.01
R7146	T11064	T11058	punct	=,0.01
R7147	T11065	T11058	cc	and,0.01
R7148	T11066	T11067	prep	for,0.03
R7149	T11067	T11058	conj	0.03,0.01
R7150	T11068	T11069	compound	phospholipid,secretion
R7151	T11069	T11066	pobj	secretion,for
R7152	T11070	T11067	nsubj	P,0.03
R7153	T11071	T11067	punct	=,0.03
R7154	T11072	T11045	punct	),8c
R7155	T11073	T11015	punct	.,showed
R7156	T11075	T11076	aux	To,investigate
R7157	T11076	T11077	advcl	investigate,infused
R7158	T11078	T11079	mark	whether,restored
R7159	T11079	T11076	ccomp	restored,investigate
R7160	T11080	T11081	det	the,defect
R7161	T11081	T11079	nsubjpass	defect,restored
R7162	T11082	T11081	prep	in,defect
R7163	T11083	T11084	amod	biliary,secretion
R7164	T11084	T11082	pobj	secretion,in
R7165	T11085	T11084	compound	sterol,secretion
R7166	T11086	T11079	aux	could,restored
R7167	T11087	T11079	auxpass	be,restored
R7168	T11088	T11079	punct	(,restored
R7169	T11089	T11079	advmod	partly,restored
R7170	T11090	T11079	punct	),restored
R7171	T11091	T11079	prep	by,restored
R7172	T11092	T11093	amod	forced,secretion
R7173	T11093	T11091	pobj	secretion,by
R7174	T11094	T11095	amod	biliary,sterol
R7175	T11095	T11093	compound	sterol,secretion
R7176	T11096	T11077	punct	", ",infused
R7177	T11097	T11077	nsubj	we,infused
R7178	T11098	T11077	dobj	mice,infused
R7179	T11099	T11077	prep	with,infused
R7180	T11100	T11101	amod	stepwise,increasing
R7181	T11101	T11102	amod	increasing,doses
R7182	T11102	T11099	pobj	doses,with
R7183	T11103	T11102	prep	of,doses
R7184	T11104	T11105	amod	tauroursodeoxycholic,acid
R7185	T11105	T11103	pobj	acid,of
R7186	T11106	T11105	punct	(,acid
R7187	T11107	T11105	appos	TUDC,acid
R7188	T11108	T11077	punct	),infused
R7189	T11109	T11077	punct	.,infused
R7190	T11111	T11112	nsubjpass	Mice,depleted
R7191	T11113	T11112	auxpass	were,depleted
R7192	T11114	T11112	advmod	first,depleted
R7193	T11115	T11112	prep	of,depleted
R7194	T11116	T11117	poss	their,pools
R7195	T11117	T11115	pobj	pools,of
R7196	T11118	T11117	amod	endogenous,pools
R7197	T11119	T11120	compound	bile,salt
R7198	T11120	T11117	compound	salt,pools
R7199	T11121	T11112	prep	for,depleted
R7200	T11122	T11123	nummod	90,min
R7201	T11123	T11121	pobj	min,for
R7202	T11124	T11112	cc	and,depleted
R7203	T11125	T11126	advmod	subsequently,infused
R7204	T11126	T11112	conj	infused,depleted
R7205	T11127	T11126	prep	via,infused
R7206	T11128	T11129	det	the,vein
R7207	T11129	T11127	pobj	vein,via
R7208	T11130	T11129	amod	jugular,vein
R7209	T11131	T11126	prep	with,infused
R7210	T11132	T11133	amod	increasing,doses
R7211	T11133	T11131	pobj	doses,with
R7212	T11134	T11133	prep	of,doses
R7213	T11135	T11134	pobj	TUDC,of
R7214	T11136	T11112	punct	.,depleted
R7215	T11138	T11139	mark	As,shown
R7216	T11139	T11140	advcl	shown,was
R7217	T11141	T11139	prep	in,shown
R7218	T11142	T11143	compound	Figure,8d
R7219	T11143	T11141	pobj	8d,in
R7220	T11144	T11140	punct	", ",was
R7221	T11145	T11146	compound	bile,salt
R7222	T11146	T11147	compound	salt,secretion
R7223	T11147	T11140	nsubj	secretion,was
R7224	T11148	T11149	neg	no,different
R7225	T11149	T11140	acomp	different,was
R7226	T11150	T11140	prep	between,was
R7227	T11151	T11152	det	the,genotypes
R7228	T11152	T11150	pobj	genotypes,between
R7229	T11153	T11152	amod	different,genotypes
R7230	T11154	T11140	prep	during,was
R7231	T11155	T11156	det	the,depletion
R7232	T11156	T11154	pobj	depletion,during
R7233	T11157	T11156	cc	or,depletion
R7234	T11158	T11159	det	the,infusion
R7235	T11159	T11161	compound	infusion,phases
R7236	T11160	T11159	compound	TUDC,infusion
R7237	T11161	T11156	conj	phases,depletion
R7238	T11162	T11156	prep	of,depletion
R7239	T11163	T11164	det	the,experiment
R7240	T11164	T11162	pobj	experiment,of
R7241	T11165	T11140	punct	.,was
R7242	T11167	T11168	advmod	However,emerged
R7243	T11169	T11168	punct	", ",emerged
R7244	T11170	T11171	det	a,pattern
R7245	T11171	T11168	nsubj	pattern,emerged
R7246	T11172	T11171	amod	different,pattern
R7247	T11173	T11168	prep	for,emerged
R7248	T11174	T11175	det	the,secretion
R7249	T11175	T11173	pobj	secretion,for
R7250	T11176	T11175	prep	of,secretion
R7251	T11177	T11176	pobj	phospholipid,of
R7252	T11178	T11179	punct	(,8f
R7253	T11179	T11168	parataxis	8f,emerged
R7254	T11180	T11179	compound	Figure,8f
R7255	T11181	T11179	punct	),8f
R7256	T11182	T11168	punct	.,emerged
R7257	T11184	T11185	expl	There,was
R7258	T11186	T11187	det	no,difference
R7259	T11187	T11185	attr	difference,was
R7260	T11188	T11187	prep	between,difference
R7261	T11189	T11190	amod	wild,type
R7262	T11190	T11192	nmod	type,mice
R7263	T11191	T11190	punct	-,type
R7264	T11192	T11188	pobj	mice,between
R7265	T11193	T11190	cc	and,type
R7266	T11194	T11190	conj	heterozygous,type
R7267	T11195	T11185	punct	", ",was
R7268	T11196	T11185	cc	but,was
R7269	T11197	T11198	compound	knockout,mice
R7270	T11198	T11199	nsubj	mice,showed
R7271	T11199	T11185	conj	showed,was
R7272	T11200	T11201	det	a,trend
R7273	T11201	T11199	dobj	trend,showed
R7274	T11202	T11201	prep	of,trend
R7275	T11203	T11204	amod	lower,secretion
R7276	T11204	T11202	pobj	secretion,of
R7277	T11205	T11204	compound	phospholipid,secretion
R7278	T11206	T11199	prep	during,showed
R7279	T11207	T11208	preconj	both,phase
R7280	T11208	T11206	pobj	phase,during
R7281	T11209	T11208	det	the,phase
R7282	T11210	T11208	nmod	depletion,phase
R7283	T11211	T11210	cc	and,depletion
R7284	T11212	T11210	conj	infusion,depletion
R7285	T11213	T11199	punct	.,showed
R7286	T11215	T11216	advmod	Even,more
R7287	T11216	T11217	advmod	more,dramatic
R7288	T11217	T11218	acomp	dramatic,was
R7289	T11219	T11220	det	the,effect
R7290	T11220	T11218	nsubj	effect,was
R7291	T11221	T11220	prep	on,effect
R7292	T11222	T11223	amod	biliary,sterol
R7293	T11223	T11224	compound	sterol,secretion
R7294	T11224	T11221	pobj	secretion,on
R7295	T11225	T11226	mark	although,determined
R7296	T11226	T11218	advcl	determined,was
R7297	T11227	T11228	det	the,contents
R7298	T11228	T11226	nsubjpass	contents,determined
R7299	T11229	T11228	compound	sterol,contents
R7300	T11230	T11226	auxpass	were,determined
R7301	T11231	T11226	advmod	enzymatically,determined
R7302	T11232	T11226	cc	and,determined
R7303	T11233	T11234	det	no,distinction
R7304	T11234	T11235	nsubjpass	distinction,made
R7305	T11235	T11226	conj	made,determined
R7306	T11236	T11235	auxpass	is,made
R7307	T11237	T11235	prep	as,made
R7308	T11238	T11237	prep	to,as
R7309	T11239	T11240	mark	whether,is
R7310	T11240	T11238	pcomp	is,to
R7311	T11241	T11240	nsubj	this,is
R7312	T11242	T11240	attr	sitosterol,is
R7313	T11243	T11242	cc	or,sitosterol
R7314	T11244	T11242	conj	cholesterol,sitosterol
R7315	T11245	T11246	punct	(,8e
R7316	T11246	T11218	parataxis	8e,was
R7317	T11247	T11246	compound	Figure,8e
R7318	T11248	T11246	punct	", ",8e
R7319	T11249	T11246	cc	but,8e
R7320	T11250	T11246	conj	see,8e
R7321	T11251	T11250	advmod	below,see
R7322	T11252	T11246	punct	),8e
R7323	T11253	T11218	punct	.,was
R7324	T11255	T11256	prep	In,noted
R7325	T11257	T11258	det	the,mice
R7326	T11258	T11255	pobj	mice,In
R7327	T11259	T11258	compound	knockout,mice
R7328	T11260	T11256	punct	", ",noted
R7329	T11261	T11262	advmod	almost,secretion
R7330	T11262	T11256	nsubjpass	secretion,noted
R7331	T11263	T11262	det	no,secretion
R7332	T11264	T11262	amod	stimulated,secretion
R7333	T11265	T11262	compound	sterol,secretion
R7334	T11266	T11256	auxpass	was,noted
R7335	T11267	T11256	punct	", ",noted
R7336	T11268	T11256	prep	with,noted
R7337	T11269	T11270	det	an,phenotype
R7338	T11270	T11268	pobj	phenotype,with
R7339	T11271	T11270	amod	intermediate,phenotype
R7340	T11272	T11270	prep	in,phenotype
R7341	T11273	T11274	det	the,mice
R7342	T11274	T11272	pobj	mice,in
R7343	T11275	T11274	amod	heterozygous,mice
R7344	T11276	T11256	punct	.,noted
R7345	T11278	T11279	prep	In,increased
R7346	T11280	T11281	det	the,mice
R7347	T11281	T11278	pobj	mice,In
R7348	T11282	T11281	amod	heterozygous,mice
R7349	T11283	T11279	punct	", ",increased
R7350	T11284	T11285	compound	sterol,secretion
R7351	T11285	T11279	nsubj	secretion,increased
R7352	T11286	T11287	aux	to,reach
R7353	T11287	T11279	advcl	reach,increased
R7354	T11288	T11289	det	a,level
R7355	T11289	T11287	dobj	level,reach
R7356	T11290	T11289	amod	maximal,level
R7357	T11291	T11289	prep	of,level
R7358	T11292	T11293	quantmod	about,50
R7359	T11293	T11294	nummod	50,%
R7360	T11294	T11291	pobj	%,of
R7361	T11295	T11294	prep	of,%
R7362	T11296	T11297	det	the,type
R7363	T11297	T11295	pobj	type,of
R7364	T11298	T11297	amod	wild,type
R7365	T11299	T11279	advcl	suggesting,increased
R7366	T11300	T11301	mark	that,is
R7367	T11301	T11299	ccomp	is,suggesting
R7368	T11302	T11303	compound	Abcg8,sterolin
R7369	T11303	T11301	nsubj	sterolin,is
R7370	T11304	T11303	punct	/,sterolin
R7371	T11305	T11303	punct	-,sterolin
R7372	T11306	T11303	nummod	2,sterolin
R7373	T11307	T11308	det	a,step
R7374	T11308	T11301	attr	step,is
R7375	T11309	T11310	npadvmod	rate,limiting
R7376	T11310	T11308	amod	limiting,step
R7377	T11311	T11310	punct	-,limiting
R7378	T11312	T11308	prep	for,step
R7379	T11313	T11314	amod	biliary,secretion
R7380	T11314	T11312	pobj	secretion,for
R7381	T11315	T11314	punct	',secretion
R7382	T11316	T11314	nmod	cholesterol,secretion
R7383	T11317	T11314	punct	',secretion
R7384	T11318	T11279	punct	.,increased
R7385	T11320	T11321	prep	In,remained
R7386	T11322	T11323	det	the,mice
R7387	T11323	T11320	pobj	mice,In
R7388	T11324	T11323	compound	knockout,mice
R7389	T11325	T11321	punct	", ",remained
R7390	T11326	T11327	compound	sterol,secretion
R7391	T11327	T11321	nsubj	secretion,remained
R7392	T11328	T11321	oprd	at,remained
R7393	T11329	T11330	det	a,level
R7394	T11330	T11328	pobj	level,at
R7395	T11331	T11330	amod	constant,level
R7396	T11332	T11321	prep	during,remained
R7397	T11333	T11334	det	the,phase
R7398	T11334	T11332	pobj	phase,during
R7399	T11335	T11334	compound	depletion,phase
R7400	T11336	T11321	cc	and,remained
R7401	T11337	T11321	conj	increased,remained
R7402	T11338	T11337	advmod	minimally,increased
R7403	T11339	T11337	prep	upon,increased
R7404	T11340	T11339	pobj	infusion,upon
R7405	T11341	T11340	prep	of,infusion
R7406	T11342	T11341	pobj	TUDC,of
R7407	T11343	T11321	punct	.,remained
R7408	T11345	T11346	mark	Since,secreted
R7409	T11346	T11354	advcl	secreted,were
R7410	T11347	T11348	det	the,mice
R7411	T11348	T11346	nsubj	mice,secreted
R7412	T11349	T11348	nmod	Abcg8,mice
R7413	T11350	T11349	punct	-,Abcg8
R7414	T11351	T11349	punct	/,Abcg8
R7415	T11352	T11349	punct	-,Abcg8
R7416	T11353	T11346	advmod	still,secreted
R7417	T11355	T11356	det	some,sterols
R7418	T11356	T11346	dobj	sterols,secreted
R7419	T11357	T11354	nsubj	we,were
R7420	T11358	T11354	acomp	interested,were
R7421	T11359	T11358	prep	in,interested
R7422	T11360	T11361	det	which,species
R7423	T11361	T11364	dep	species,secreted
R7424	T11362	T11363	amod	biliary,sterol
R7425	T11363	T11361	compound	sterol,species
R7426	T11364	T11359	pcomp	secreted,in
R7427	T11365	T11364	aux	were,secreted
R7428	T11366	T11364	auxpass	being,secreted
R7429	T11367	T11354	punct	.,were
R7430	T11369	T11370	advmod	Therefore,collected
R7431	T11371	T11370	nsubjpass	bile,collected
R7432	T11372	T11370	auxpass	was,collected
R7433	T11373	T11370	prep	from,collected
R7434	T11374	T11373	pobj	mice,from
R7435	T11375	T11370	prep	during,collected
R7436	T11376	T11377	det	a,infusion
R7437	T11377	T11375	pobj	infusion,during
R7438	T11378	T11377	amod	constant,infusion
R7439	T11379	T11377	prep	of,infusion
R7440	T11380	T11379	pobj	TUDC,of
R7441	T11381	T11370	cc	and,collected
R7442	T11382	T11370	conj	analyzed,collected
R7443	T11383	T11382	prep	by,analyzed
R7444	T11384	T11383	pobj	GC,by
R7445	T11385	T11370	punct	.,collected
R7446	T11387	T11388	nsubjpass	Mice,depleted
R7447	T11389	T11388	auxpass	were,depleted
R7448	T11390	T11388	advmod	first,depleted
R7449	T11391	T11388	prep	of,depleted
R7450	T11392	T11393	poss	their,pools
R7451	T11393	T11391	pobj	pools,of
R7452	T11394	T11393	amod	endogenous,pools
R7453	T11395	T11396	compound	bile,acid
R7454	T11396	T11393	compound	acid,pools
R7455	T11397	T11388	prep	for,depleted
R7456	T11398	T11399	nummod	30,minutes
R7457	T11399	T11397	pobj	minutes,for
R7458	T11400	T11401	advmod	then,infused
R7459	T11401	T11388	dep	infused,depleted
R7460	T11402	T11401	prep	with,infused
R7461	T11403	T11404	det	a,dose
R7462	T11404	T11402	pobj	dose,with
R7463	T11405	T11404	amod	continuous,dose
R7464	T11406	T11404	prep	of,dose
R7465	T11407	T11406	pobj	TUDC,of
R7466	T11408	T11409	punct	(,nmol
R7467	T11409	T11388	parataxis	nmol,depleted
R7468	T11410	T11409	nummod	"1,200",nmol
R7469	T11411	T11412	punct	/,min
R7470	T11412	T11409	prep	min,nmol
R7471	T11413	T11414	punct	/,weight
R7472	T11414	T11409	prep	weight,nmol
R7473	T11415	T11416	compound	100,g
R7474	T11416	T11414	compound	g,weight
R7475	T11417	T11414	compound	body,weight
R7476	T11418	T11409	punct	),nmol
R7477	T11419	T11388	punct	.,depleted
R7478	T11421	T11422	det	The,mice
R7479	T11422	T11424	nsubj	mice,showed
R7480	T11423	T11422	compound	knockout,mice
R7481	T11425	T11426	det	a,ability
R7482	T11426	T11424	dobj	ability,showed
R7483	T11427	T11428	advmod	significantly,diminished
R7484	T11428	T11426	amod	diminished,ability
R7485	T11429	T11430	aux	to,secrete
R7486	T11430	T11426	acl	secrete,ability
R7487	T11431	T11430	dobj	cholesterol,secrete
R7488	T11432	T11424	punct	", ",showed
R7489	T11433	T11424	cc	yet,showed
R7490	T11434	T11435	advmod	still,maintained
R7491	T11435	T11424	conj	maintained,showed
R7492	T11436	T11437	det	the,ability
R7493	T11437	T11435	dobj	ability,maintained
R7494	T11438	T11439	aux	to,secrete
R7495	T11439	T11437	acl	secrete,ability
R7496	T11440	T11439	dobj	sitosterol,secrete
R7497	T11441	T11440	cc	and,sitosterol
R7498	T11442	T11440	conj	campesterol,sitosterol
R7499	T11443	T11424	prep	compared,showed
R7500	T11444	T11443	prep	to,compared
R7501	T11445	T11446	amod	wild,type
R7502	T11446	T11448	compound	type,mice
R7503	T11447	T11446	punct	-,type
R7504	T11448	T11444	pobj	mice,to
R7505	T11449	T11450	punct	(,Figure
R7506	T11450	T11424	parataxis	Figure,showed
R7507	T11451	T11450	nummod	9,Figure
R7508	T11452	T11450	punct	),Figure
R7509	T11453	T11424	punct	.,showed
R7510	T11455	T11456	advmod	Surprisingly,tended
R7511	T11457	T11456	punct	", ",tended
R7512	T11458	T11459	det	the,mice
R7513	T11459	T11456	nsubj	mice,tended
R7514	T11460	T11459	nmod	Abcg8,mice
R7515	T11461	T11460	punct	+,Abcg8
R7516	T11462	T11460	punct	/,Abcg8
R7517	T11463	T11460	punct	-,Abcg8
R7518	T11464	T11465	aux	to,show
R7519	T11465	T11456	xcomp	show,tended
R7520	T11466	T11467	det	an,ability
R7521	T11467	T11465	dobj	ability,show
R7522	T11468	T11467	amod	increased,ability
R7523	T11469	T11470	aux	to,secrete
R7524	T11470	T11467	acl	secrete,ability
R7525	T11471	T11472	det	all,sterols
R7526	T11472	T11470	dobj	sterols,secrete
R7527	T11473	T11470	prep	above,secrete
R7528	T11474	T11475	det	the,levels
R7529	T11475	T11473	pobj	levels,above
R7530	T11476	T11475	acl	seen,levels
R7531	T11477	T11476	prep	in,seen
R7532	T11478	T11479	det	the,mice
R7533	T11479	T11477	pobj	mice,in
R7534	T11480	T11481	amod	wild,type
R7535	T11481	T11479	compound	type,mice
R7536	T11482	T11481	punct	-,type
R7537	T11483	T11456	punct	", ",tended
R7538	T11484	T11485	mark	although,was
R7539	T11485	T11456	advcl	was,tended
R7540	T11486	T11485	nsubj	this,was
R7541	T11487	T11485	neg	not,was
R7542	T11488	T11489	advmod	statistically,significant
R7543	T11489	T11485	acomp	significant,was
R7544	T11490	T11456	punct	.,tended
R7548	T12567	T12568	det	The,mechanism
R7549	T12568	T12569	nsubjpass	mechanism,elucidated
R7550	T12570	T12571	prep	by,absorbed
R7551	T12571	T12568	relcl	absorbed,mechanism
R7552	T12572	T12570	pobj	which,by
R7553	T12573	T12574	amod	dietary,cholesterol
R7554	T12574	T12571	nsubjpass	cholesterol,absorbed
R7555	T12575	T12571	auxpass	is,absorbed
R7556	T12576	T12571	advmod	specifically,absorbed
R7557	T12577	T12571	cc	and,absorbed
R7558	T12578	T12579	amod	dietary,sterols
R7559	T12579	T12581	nsubj	sterols,excluded
R7560	T12580	T12579	compound	non-cholesterol,sterols
R7561	T12581	T12571	conj	excluded,absorbed
R7562	T12582	T12581	advmod	primarily,excluded
R7563	T12583	T12568	cc	or,mechanism
R7564	T12584	T12585	det	the,mechanism
R7565	T12585	T12568	conj	mechanism,mechanism
R7566	T12586	T12585	punct	(,mechanism
R7567	T12587	T12585	nmod	s,mechanism
R7568	T12588	T12585	punct	),mechanism
R7569	T12589	T12590	prep	by,secrete
R7570	T12590	T12585	relcl	secrete,mechanism
R7571	T12591	T12589	pobj	which,by
R7572	T12592	T12593	det	the,liver
R7573	T12593	T12590	nsubj	liver,secrete
R7574	T12594	T12590	aux	can,secrete
R7575	T12595	T12590	advmod	selectively,secrete
R7576	T12596	T12590	dobj	sterols,secrete
R7577	T12597	T12569	aux	has,elucidated
R7578	T12598	T12569	neg	not,elucidated
R7579	T12599	T12569	auxpass	been,elucidated
R7580	T12600	T12569	punct	.,elucidated
R7581	T12602	T12603	nsubj	Identification,led
R7582	T12604	T12602	prep	of,Identification
R7583	T12605	T12606	det	the,defect
R7584	T12606	T12604	pobj	defect,of
R7585	T12607	T12606	amod	genetic,defect
R7586	T12608	T12606	punct	(,defect
R7587	T12609	T12606	nmod	s,defect
R7588	T12610	T12606	punct	),defect
R7589	T12611	T12606	prep	in,defect
R7590	T12612	T12613	det	a,disorder
R7591	T12613	T12611	pobj	disorder,in
R7592	T12614	T12613	amod	rare,disorder
R7593	T12615	T12613	amod	human,disorder
R7594	T12616	T12613	punct	", ",disorder
R7595	T12617	T12613	appos	sitosterolemia,disorder
R7596	T12618	T12613	punct	", ",disorder
R7597	T12619	T12620	advmod	where,disrupted
R7598	T12620	T12613	relcl	disrupted,disorder
R7599	T12621	T12622	det	these,processes
R7600	T12622	T12620	nsubjpass	processes,disrupted
R7601	T12623	T12620	auxpass	are,disrupted
R7602	T12624	T12620	advmod	specifically,disrupted
R7603	T12625	T12603	punct	", ",led
R7604	T12626	T12603	aux	may,led
R7605	T12627	T12603	advmod	finally,led
R7606	T12628	T12603	aux	have,led
R7607	T12629	T12603	prep	to,led
R7608	T12630	T12631	det	the,identification
R7609	T12631	T12629	pobj	identification,to
R7610	T12632	T12631	prep	of,identification
R7611	T12633	T12634	det	the,transporters
R7612	T12634	T12632	pobj	transporters,of
R7613	T12635	T12634	punct	',transporters
R7614	T12636	T12634	punct	',transporters
R7615	T12637	T12634	amod	responsible,transporters
R7616	T12638	T12637	prep	for,responsible
R7617	T12639	T12640	det	these,processes
R7618	T12640	T12638	pobj	processes,for
R7619	T12641	T12603	punct	.,led
R7620	T12643	T12644	amod	Complete,defects
R7621	T12644	T12645	nsubj	defects,causes
R7622	T12646	T12644	prep	in,defects
R7623	T12647	T12648	quantmod	one,two
R7624	T12648	T12650	nummod	two,genes
R7625	T12649	T12648	quantmod	of,two
R7626	T12650	T12646	pobj	genes,in
R7627	T12651	T12650	punct	(,genes
R7628	T12652	T12650	cc	but,genes
R7629	T12653	T12652	neg	not,but
R7630	T12654	T12650	conj	both,genes
R7631	T12655	T12644	punct	),defects
R7632	T12656	T12644	punct	", ",defects
R7633	T12657	T12644	acl	organized,defects
R7634	T12658	T12657	prep	in,organized
R7635	T12659	T12660	det	a,configuration
R7636	T12660	T12658	pobj	configuration,in
R7637	T12661	T12660	nmod	head,configuration
R7638	T12662	T12661	punct	-,head
R7639	T12663	T12661	prep	to,head
R7640	T12664	T12663	punct	-,to
R7641	T12665	T12663	pobj	head,to
R7642	T12666	T12657	prep	at,organized
R7643	T12667	T12668	det	the,locus
R7644	T12668	T12666	pobj	locus,at
R7645	T12669	T12668	compound	STSL,locus
R7646	T12670	T12645	punct	", ",causes
R7647	T12671	T12645	dobj	sitosterolemia,causes
R7648	T12672	T12645	punct	.,causes
R7649	T12674	T12675	nsubj	We,report
R7650	T12676	T12677	det	a,model
R7651	T12677	T12675	dobj	model,report
R7652	T12678	T12677	compound	mouse,model
R7653	T12679	T12677	prep	of,model
R7654	T12680	T12679	pobj	sitosterolemia,of
R7655	T12681	T12677	punct	", ",model
R7656	T12682	T12677	prep	with,model
R7657	T12683	T12684	det	a,defect
R7658	T12684	T12682	pobj	defect,with
R7659	T12685	T12684	amod	selective,defect
R7660	T12686	T12685	punct	", ",selective
R7661	T12687	T12685	cc	but,selective
R7662	T12688	T12685	conj	complete,selective
R7663	T12689	T12684	punct	", ",defect
R7664	T12690	T12684	prep	in,defect
R7665	T12691	T12690	pobj	one,in
R7666	T12692	T12691	prep	of,one
R7667	T12693	T12694	det	these,genes
R7668	T12694	T12692	pobj	genes,of
R7669	T12695	T12691	punct	", ",one
R7670	T12696	T12691	appos	Abcg8,one
R7671	T12697	T12675	punct	.,report
R7672	T12699	T12700	det	This,mouse
R7673	T12700	T12701	nsubj	mouse,reflects
R7674	T12702	T12703	det	the,defects
R7675	T12703	T12701	dobj	defects,reflects
R7676	T12704	T12703	amod	known,defects
R7677	T12705	T12703	acl	described,defects
R7678	T12706	T12705	prep	in,described
R7679	T12707	T12708	amod	human,sitosterolemia
R7680	T12708	T12706	pobj	sitosterolemia,in
R7681	T12709	T12710	punct	[,42
R7682	T12710	T12701	parataxis	42,reflects
R7683	T12711	T12710	nummod	5,42
R7684	T12712	T12710	punct	",",42
R7685	T12713	T12710	nummod	7,42
R7686	T12714	T12710	punct	",",42
R7687	T12715	T12710	punct	],42
R7688	T12716	T12701	punct	.,reflects
R7689	T12718	T12719	nsubj	Plasma,have
R7690	T12720	T12718	cc	and,Plasma
R7691	T12721	T12722	det	all,tissues
R7692	T12722	T12718	conj	tissues,Plasma
R7693	T12723	T12722	punct	", ",tissues
R7694	T12724	T12722	advmod	apart,tissues
R7695	T12725	T12724	prep	from,apart
R7696	T12726	T12727	det	the,brain
R7697	T12727	T12725	pobj	brain,from
R7698	T12728	T12719	punct	", ",have
R7699	T12729	T12730	advmod	significantly,elevated
R7700	T12730	T12731	amod	elevated,levels
R7701	T12731	T12719	dobj	levels,have
R7702	T12732	T12731	compound	sitosterol,levels
R7703	T12733	T12719	punct	.,have
R7704	T12735	T12736	amod	Homozygous,mice
R7705	T12736	T12738	nsubj	mice,are
R7706	T12737	T12736	compound	knockout,mice
R7707	T12739	T12738	acomp	viable,are
R7708	T12740	T12739	punct	", ",viable
R7709	T12741	T12739	conj	fertile,viable
R7710	T12742	T12741	cc	and,fertile
R7711	T12743	T12741	conj	sitosterolemic,fertile
R7712	T12744	T12738	punct	.,are
R7713	T12746	T12747	advmod	However,seems
R7714	T12748	T12747	punct	", ",seems
R7715	T12749	T12747	nsubj	fertility,seems
R7716	T12750	T12751	aux	to,reduced
R7717	T12751	T12747	xcomp	reduced,seems
R7718	T12752	T12751	auxpass	be,reduced
R7719	T12753	T12754	advmod	when,bred
R7720	T12754	T12751	advcl	bred,reduced
R7721	T12755	T12756	amod	homozygous,mice
R7722	T12756	T12754	nsubjpass	mice,bred
R7723	T12757	T12754	auxpass	are,bred
R7724	T12758	T12754	advmod	together,bred
R7725	T12759	T12760	punct	(,S
R7726	T12760	T12751	meta	S,reduced
R7727	T12761	T12760	nmod	Patel,S
R7728	T12762	T12760	cc	and,S
R7729	T12763	T12760	conj	J,S
R7730	T12764	T12763	nmod	Oatis,J
R7731	T12765	T12763	punct	", ",J
R7732	T12766	T12767	amod	unpublished,observation
R7733	T12767	T12763	conj	observation,J
R7734	T12768	T12767	punct	),observation
R7735	T12769	T12747	punct	.,seems
R7736	T12771	T12772	det	This,model
R7737	T12772	T12773	nsubj	model,reflects
R7738	T12774	T12775	amod	many,changes
R7739	T12775	T12773	dobj	changes,reflects
R7740	T12776	T12775	amod	other,changes
R7741	T12777	T12775	amod	observed,changes
R7742	T12778	T12775	acl	described,changes
R7743	T12779	T12778	prep	in,described
R7744	T12780	T12781	amod	limited,studies
R7745	T12781	T12779	pobj	studies,in
R7746	T12782	T12781	prep	in,studies
R7747	T12783	T12782	pobj	humans,in
R7748	T12784	T12773	punct	.,reflects
R7749	T12786	T12787	prep	For,reported
R7750	T12788	T12786	pobj	example,For
R7751	T12789	T12787	punct	", ",reported
R7752	T12790	T12787	prep	in,reported
R7753	T12791	T12790	pobj	humans,in
R7754	T12792	T12787	punct	", ",reported
R7755	T12793	T12794	det	the,activity
R7756	T12794	T12787	nsubjpass	activity,reported
R7757	T12795	T12794	prep	of,activity
R7758	T12796	T12797	compound	HMG,CoA
R7759	T12797	T12799	compound	CoA,reductase
R7760	T12798	T12797	punct	-,CoA
R7761	T12799	T12795	pobj	reductase,of
R7762	T12800	T12799	cc	and,reductase
R7763	T12801	T12799	conj	CYP7a1,reductase
R7764	T12802	T12787	aux	have,reported
R7765	T12803	T12787	auxpass	been,reported
R7766	T12804	T12805	aux	to,be
R7767	T12805	T12787	xcomp	be,reported
R7768	T12806	T12805	acomp	low,be
R7769	T12807	T12808	punct	[,39
R7770	T12808	T12787	parataxis	39,reported
R7771	T12809	T12808	nummod	7,39
R7772	T12810	T12808	punct	",",39
R7773	T12811	T12808	punct	],39
R7774	T12812	T12787	punct	.,reported
R7775	T12814	T12815	prep	In,show
R7776	T12816	T12817	det	this,study
R7777	T12817	T12814	pobj	study,In
R7778	T12818	T12815	punct	", ",show
R7779	T12819	T12815	nsubj	we,show
R7780	T12820	T12821	mark	that,reduced
R7781	T12821	T12815	ccomp	reduced,show
R7782	T12822	T12823	det	the,mRNA
R7783	T12823	T12821	nsubjpass	mRNA,reduced
R7784	T12824	T12823	cc	and,mRNA
R7785	T12825	T12826	compound	enzyme,activities
R7786	T12826	T12823	conj	activities,mRNA
R7787	T12827	T12823	prep	in,mRNA
R7788	T12828	T12829	det	the,livers
R7789	T12829	T12827	pobj	livers,in
R7790	T12830	T12829	prep	from,livers
R7791	T12831	T12832	compound	knockout,animals
R7792	T12832	T12830	pobj	animals,from
R7793	T12833	T12821	auxpass	are,reduced
R7794	T12834	T12821	advmod	also,reduced
R7795	T12835	T12821	advmod	significantly,reduced
R7796	T12836	T12815	punct	.,show
R7797	T12838	T12839	advmod	Additionally,reduced
R7798	T12840	T12839	punct	", ",reduced
R7799	T12841	T12842	det	the,activity
R7800	T12842	T12839	nsubjpass	activity,reduced
R7801	T12843	T12842	prep	of,activity
R7802	T12844	T12845	det	the,enzyme
R7803	T12845	T12843	pobj	enzyme,of
R7804	T12846	T12847	npadvmod	rate,limiting
R7805	T12847	T12845	amod	limiting,enzyme
R7806	T12848	T12847	punct	-,limiting
R7807	T12849	T12845	prep	for,enzyme
R7808	T12850	T12851	compound	bile,acid
R7809	T12851	T12852	compound	acid,synthesis
R7810	T12852	T12849	pobj	synthesis,for
R7811	T12853	T12845	punct	", ",enzyme
R7812	T12854	T12845	appos	CYP7a1,enzyme
R7813	T12855	T12839	punct	", ",reduced
R7814	T12856	T12839	auxpass	is,reduced
R7815	T12857	T12839	punct	", ",reduced
R7816	T12858	T12859	mark	although,noted
R7817	T12859	T12839	advcl	noted,reduced
R7818	T12860	T12861	det	no,changes
R7819	T12861	T12859	nsubjpass	changes,noted
R7820	T12862	T12861	prep	at,changes
R7821	T12863	T12864	det	the,level
R7822	T12864	T12862	pobj	level,at
R7823	T12865	T12864	compound	mRNA,level
R7824	T12866	T12859	auxpass	are,noted
R7825	T12867	T12839	punct	.,reduced
R7826	T12869	T12870	nsubj	This,is
R7827	T12871	T12870	advmod	also,is
R7828	T12872	T12870	prep	in,is
R7829	T12873	T12872	pobj	keeping,in
R7830	T12874	T12873	prep	with,keeping
R7831	T12875	T12876	amod	previous,studies
R7832	T12876	T12874	pobj	studies,with
R7833	T12877	T12878	dep	that,show
R7834	T12878	T12876	relcl	show,studies
R7835	T12879	T12880	mark	that,is
R7836	T12880	T12878	ccomp	is,show
R7837	T12881	T12880	nsubj	sitosterol,is
R7838	T12882	T12883	det	a,inhibitor
R7839	T12883	T12880	attr	inhibitor,is
R7840	T12884	T12883	amod	direct,inhibitor
R7841	T12885	T12883	prep	of,inhibitor
R7842	T12886	T12887	det	this,enzyme
R7843	T12887	T12885	pobj	enzyme,of
R7844	T12888	T12889	advmod	in,vitro
R7845	T12889	T12880	advmod	vitro,is
R7846	T12890	T12891	punct	[,39
R7847	T12891	T12870	parataxis	39,is
R7848	T12892	T12891	punct	],39
R7849	T12893	T12870	punct	.,is
R7850	T12895	T12896	amod	Biliary,sterols
R7851	T12896	T12897	nsubj	sterols,were
R7852	T12898	T12896	prep	of,sterols
R7853	T12899	T12900	npadvmod	Abcg8,deficient
R7854	T12900	T12902	amod	deficient,mice
R7855	T12901	T12900	punct	-,deficient
R7856	T12902	T12898	pobj	mice,of
R7857	T12903	T12904	advmod	dramatically,different
R7858	T12904	T12897	acomp	different,were
R7859	T12905	T12897	prep	compared,were
R7860	T12906	T12905	prep	to,compared
R7861	T12907	T12908	amod	wild,type
R7862	T12908	T12910	compound	type,mice
R7863	T12909	T12908	punct	-,type
R7864	T12910	T12906	pobj	mice,to
R7865	T12911	T12897	punct	.,were
R7866	T12913	T12914	nsubj	Measurement,demonstrated
R7867	T12915	T12913	prep	of,Measurement
R7868	T12916	T12917	amod	biliary,sterol
R7869	T12917	T12918	compound	sterol,rates
R7870	T12918	T12915	pobj	rates,of
R7871	T12919	T12918	compound	secretion,rates
R7872	T12920	T12918	prep	in,rates
R7873	T12921	T12922	nmod	Abcg8,mice
R7874	T12922	T12920	pobj	mice,in
R7875	T12923	T12921	punct	-,Abcg8
R7876	T12924	T12921	punct	/,Abcg8
R7877	T12925	T12921	punct	-,Abcg8
R7878	T12926	T12927	det	a,failure
R7879	T12927	T12914	dobj	failure,demonstrated
R7880	T12928	T12929	aux	to,increase
R7881	T12929	T12927	acl	increase,failure
R7882	T12930	T12931	compound	sterol,secretion
R7883	T12931	T12929	dobj	secretion,increase
R7884	T12932	T12931	prep	into,secretion
R7885	T12933	T12932	pobj	bile,into
R7886	T12934	T12927	prep	despite,failure
R7887	T12935	T12936	amod	exogenous,infusion
R7888	T12936	T12934	pobj	infusion,despite
R7889	T12937	T12936	prep	of,infusion
R7890	T12938	T12939	compound	bile,acids
R7891	T12939	T12937	pobj	acids,of
R7892	T12940	T12914	punct	.,demonstrated
R7893	T12942	T12943	advmod	Furthermore,results
R7894	T12944	T12943	punct	", ",results
R7895	T12945	T12946	amod	complete,loss
R7896	T12946	T12943	nsubj	loss,results
R7897	T12947	T12946	prep	of,loss
R7898	T12948	T12949	compound	Abcg8,sterolin
R7899	T12949	T12947	pobj	sterolin,of
R7900	T12950	T12949	punct	/,sterolin
R7901	T12951	T12949	punct	-,sterolin
R7902	T12952	T12949	nummod	2,sterolin
R7903	T12953	T12943	prep	in,results
R7904	T12954	T12955	det	an,inability
R7905	T12955	T12953	pobj	inability,in
R7906	T12956	T12957	aux	to,secrete
R7907	T12957	T12955	acl	secrete,inability
R7908	T12958	T12957	dobj	cholesterol,secrete
R7909	T12959	T12957	prep	into,secrete
R7910	T12960	T12959	pobj	bile,into
R7911	T12961	T12943	punct	", ",results
R7912	T12962	T12963	mark	although,seemed
R7913	T12963	T12943	advcl	seemed,results
R7914	T12964	T12963	nsubj	secretion,seemed
R7915	T12965	T12964	prep	of,secretion
R7916	T12966	T12965	pobj	sitosterol,of
R7917	T12967	T12968	aux	to,preserved
R7918	T12968	T12963	xcomp	preserved,seemed
R7919	T12969	T12968	auxpass	be,preserved
R7920	T12970	T12943	punct	.,results
R7921	T12972	T12973	advmod	Arguably,considered
R7922	T12974	T12973	punct	", ",considered
R7923	T12975	T12976	mark	since,are
R7924	T12976	T12973	advcl	are,considered
R7925	T12977	T12978	det	the,pools
R7926	T12978	T12976	nsubj	pools,are
R7927	T12979	T12978	compound	body,pools
R7928	T12980	T12978	cc	and,pools
R7929	T12981	T12982	compound	plasma,sitosterol
R7930	T12982	T12983	compound	sitosterol,levels
R7931	T12983	T12978	conj	levels,pools
R7932	T12984	T12978	prep	in,pools
R7933	T12985	T12986	det	the,mice
R7934	T12986	T12984	pobj	mice,in
R7935	T12987	T12986	compound	knockout,mice
R7936	T12988	T12989	advmod	so,considerably
R7937	T12989	T12976	advmod	considerably,are
R7938	T12990	T12976	acomp	elevated,are
R7939	T12991	T12973	punct	", ",considered
R7940	T12992	T12973	advmod	perhaps,considered
R7941	T12993	T12994	det	the,levels
R7942	T12994	T12973	nsubjpass	levels,considered
R7943	T12995	T12996	amod	biliary,sitosterol
R7944	T12996	T12994	compound	sitosterol,levels
R7945	T12997	T12973	aux	could,considered
R7946	T12998	T12973	auxpass	be,considered
R7947	T12999	T13000	aux	to,be
R7948	T13000	T12973	xcomp	be,considered
R7949	T13001	T13002	advmod	inappropriately,low
R7950	T13002	T13000	acomp	low,be
R7951	T13003	T12973	punct	.,considered
R7952	T13005	T13006	prep	Despite,suggests
R7953	T13007	T13008	det	this,reservation
R7954	T13008	T13005	pobj	reservation,Despite
R7955	T13009	T13006	punct	", ",suggests
R7956	T13010	T13011	det	the,finding
R7957	T13011	T13006	nsubj	finding,suggests
R7958	T13012	T13013	mark	that,is
R7959	T13013	T13011	acl	is,finding
R7960	T13014	T13013	nsubj	sitosterol,is
R7961	T13015	T13013	acomp	present,is
R7962	T13016	T13015	prep	in,present
R7963	T13017	T13018	det	the,bile
R7964	T13018	T13016	pobj	bile,in
R7965	T13019	T13020	mark	that,secreted
R7966	T13020	T13006	ccomp	secreted,suggests
R7967	T13021	T13022	compound	plant,sterols
R7968	T13022	T13020	nsubjpass	sterols,secreted
R7969	T13023	T13020	aux	may,secreted
R7970	T13024	T13020	auxpass	be,secreted
R7971	T13025	T13020	advmod	independent,secreted
R7972	T13026	T13025	prep	of,independent
R7973	T13027	T13028	compound	Abcg8,sterolin
R7974	T13028	T13026	pobj	sterolin,of
R7975	T13029	T13028	punct	/,sterolin
R7976	T13030	T13028	punct	-,sterolin
R7977	T13031	T13028	nummod	2,sterolin
R7978	T13032	T13006	punct	.,suggests
R7979	T13034	T13035	det	The,ability
R7980	T13035	T13036	nsubjpass	ability,impaired
R7981	T13037	T13035	prep	of,ability
R7982	T13038	T13039	nmod	Abcg8,mice
R7983	T13039	T13037	pobj	mice,of
R7984	T13040	T13038	punct	-,Abcg8
R7985	T13041	T13038	punct	/,Abcg8
R7986	T13042	T13038	punct	-,Abcg8
R7987	T13043	T13044	aux	to,secrete
R7988	T13044	T13035	acl	secrete,ability
R7989	T13045	T13046	compound	bile,salt
R7990	T13046	T13044	dobj	salt,secrete
R7991	T13047	T13046	cc	and,salt
R7992	T13048	T13046	conj	phospholipid,salt
R7993	T13049	T13044	prep	into,secrete
R7994	T13050	T13049	pobj	bile,into
R7995	T13051	T13052	mark	as,compared
R7996	T13052	T13044	advcl	compared,secrete
R7997	T13053	T13052	prep	to,compared
R7998	T13054	T13055	amod	wild,type
R7999	T13055	T13057	compound	type,mice
R8000	T13056	T13055	punct	-,type
R8001	T13057	T13053	pobj	mice,to
R8002	T13058	T13036	auxpass	was,impaired
R8003	T13059	T13036	neg	not,impaired
R8004	T13060	T13036	advmod	significantly,impaired
R8005	T13061	T13036	punct	.,impaired
R8006	T13063	T13064	advmod	Collectively,suggest
R8007	T13065	T13066	det	these,data
R8008	T13066	T13064	nsubj	data,suggest
R8009	T13067	T13068	mark	that,is
R8010	T13068	T13064	ccomp	is,suggest
R8011	T13069	T13070	compound	Abcg8,sterolin
R8012	T13070	T13068	nsubj	sterolin,is
R8013	T13071	T13070	punct	/,sterolin
R8014	T13072	T13070	punct	-,sterolin
R8015	T13073	T13070	nummod	2,sterolin
R8016	T13074	T13068	acomp	necessary,is
R8017	T13075	T13074	prep	for,necessary
R8018	T13076	T13077	amod	hepatobiliary,secretion
R8019	T13077	T13075	pobj	secretion,for
R8020	T13078	T13077	compound	cholesterol,secretion
R8021	T13079	T13077	prep	in,secretion
R8022	T13080	T13079	pobj	mice,in
R8023	T13081	T13064	punct	.,suggest
R8024	T13083	T13084	nmod	Abcg8,mice
R8025	T13084	T13088	nsubj	mice,appeared
R8026	T13085	T13083	punct	-,Abcg8
R8027	T13086	T13083	punct	/,Abcg8
R8028	T13087	T13083	punct	-,Abcg8
R8029	T13089	T13088	oprd	normal,appeared
R8030	T13090	T13089	cc	and,normal
R8031	T13091	T13089	conj	healthy,normal
R8032	T13092	T13088	advcl	maintained,appeared
R8033	T13093	T13092	prep	on,maintained
R8034	T13094	T13095	det	a,diet
R8035	T13095	T13093	pobj	diet,on
R8036	T13096	T13095	amod	regular,diet
R8037	T13097	T13098	compound	rodent,chow
R8038	T13098	T13095	compound	chow,diet
R8039	T13099	T13088	punct	.,appeared
R8040	T13101	T13102	poss	Their,features
R8041	T13102	T13104	nsubj	features,are
R8042	T13103	T13102	amod	phenotypic,features
R8043	T13105	T13106	advmod	very,similar
R8044	T13106	T13104	acomp	similar,are
R8045	T13107	T13106	prep	to,similar
R8046	T13108	T13107	pobj	those,to
R8047	T13109	T13110	advmod	recently,reported
R8048	T13110	T13108	acl	reported,those
R8049	T13111	T13110	prep	for,reported
R8050	T13112	T13113	det	a,mouse
R8051	T13113	T13111	pobj	mouse,for
R8052	T13114	T13113	amod	deficient,mouse
R8053	T13115	T13114	prep	in,deficient
R8054	T13116	T13117	det	both,sterolins
R8055	T13117	T13115	pobj	sterolins,in
R8056	T13118	T13117	advmod	simultaneously,sterolins
R8057	T13119	T13120	punct	[,20
R8058	T13120	T13104	parataxis	20,are
R8059	T13121	T13120	nummod	7,20
R8060	T13122	T13120	punct	",",20
R8061	T13123	T13120	punct	],20
R8062	T13124	T13104	punct	.,are
R8063	T13126	T13127	nmod	Plasma,levels
R8064	T13127	T13132	nsubjpass	levels,increased
R8065	T13128	T13126	cc	and,Plasma
R8066	T13129	T13126	conj	hepatic,Plasma
R8067	T13130	T13131	compound	plant,sterol
R8068	T13131	T13127	compound	sterol,levels
R8069	T13133	T13127	prep	in,levels
R8070	T13134	T13133	pobj	Abcg8,in
R8071	T13135	T13134	punct	-,Abcg8
R8072	T13136	T13134	punct	/,Abcg8
R8073	T13137	T13134	punct	-,Abcg8
R8074	T13138	T13134	cc	and,Abcg8
R8075	T13139	T13140	compound	Abcg5,Abcg8
R8076	T13140	T13142	compound	Abcg8,knockout
R8077	T13141	T13140	punct	/,Abcg8
R8078	T13142	T13134	conj	knockout,Abcg8
R8079	T13143	T13132	auxpass	are,increased
R8080	T13144	T13132	advmod	similarly,increased
R8081	T13145	T13132	prep	with,increased
R8082	T13146	T13147	amod	marked,decreases
R8083	T13147	T13145	pobj	decreases,with
R8084	T13148	T13147	prep	in,decreases
R8085	T13149	T13150	compound	cholesterol,levels
R8086	T13150	T13148	pobj	levels,in
R8087	T13151	T13132	punct	.,increased
R8088	T13153	T13154	advmod	Interestingly,were
R8089	T13155	T13154	punct	", ",were
R8090	T13156	T13157	compound	plasma,triglyceride
R8091	T13157	T13158	compound	triglyceride,levels
R8092	T13158	T13154	nsubj	levels,were
R8093	T13159	T13158	prep	of,levels
R8094	T13160	T13161	det	the,mice
R8095	T13161	T13159	pobj	mice,of
R8096	T13162	T13163	nmod	Abcg8,-
R8097	T13163	T13161	punct	-,mice
R8098	T13164	T13163	punct	-,-
R8099	T13165	T13163	punct	/,-
R8100	T13166	T13167	advmod	slightly,higher
R8101	T13167	T13154	acomp	higher,were
R8102	T13168	T13167	prep	than,higher
R8103	T13169	T13170	amod	wild,type
R8104	T13170	T13172	compound	type,animals
R8105	T13171	T13170	punct	-,type
R8106	T13172	T13168	pobj	animals,than
R8107	T13173	T13154	cc	and,were
R8108	T13174	T13175	det	this,triglyceride
R8109	T13175	T13177	nsubjpass	triglyceride,carried
R8110	T13176	T13175	amod	increased,triglyceride
R8111	T13177	T13154	conj	carried,were
R8112	T13178	T13177	auxpass	is,carried
R8113	T13179	T13177	prep	in,carried
R8114	T13180	T13181	det	the,range
R8115	T13181	T13179	pobj	range,in
R8116	T13182	T13183	compound	LDL,fraction
R8117	T13183	T13181	compound	fraction,range
R8118	T13184	T13177	punct	", ",carried
R8119	T13185	T13186	mark	as,measured
R8120	T13186	T13177	advcl	measured,carried
R8121	T13187	T13186	prep	by,measured
R8122	T13188	T13187	pobj	FPLC,by
R8123	T13189	T13177	punct	.,carried
R8124	T13191	T13192	det	The,significance
R8125	T13192	T13193	nsubj	significance,is
R8126	T13194	T13192	prep	of,significance
R8127	T13195	T13194	pobj	this,of
R8128	T13196	T13193	neg	not,is
R8129	T13197	T13193	acomp	clear,is
R8130	T13198	T13193	punct	", ",is
R8131	T13199	T13200	mark	although,failed
R8132	T13200	T13193	advcl	failed,is
R8133	T13201	T13202	amod	preliminary,analyses
R8134	T13202	T13200	nsubj	analyses,failed
R8135	T13203	T13204	compound	SDS,PAGE
R8136	T13204	T13202	compound	PAGE,analyses
R8137	T13205	T13204	punct	-,PAGE
R8138	T13206	T13202	prep	of,analyses
R8139	T13207	T13208	predet	all,fractions
R8140	T13208	T13206	pobj	fractions,of
R8141	T13209	T13208	det	the,fractions
R8142	T13210	T13211	aux	to,reveal
R8143	T13211	T13200	xcomp	reveal,failed
R8144	T13212	T13213	det	any,differences
R8145	T13213	T13211	dobj	differences,reveal
R8146	T13214	T13213	prep	between,differences
R8147	T13215	T13216	amod	wild,type
R8148	T13216	T13218	nmod	type,samples
R8149	T13217	T13216	punct	-,type
R8150	T13218	T13214	pobj	samples,between
R8151	T13219	T13216	cc	and,type
R8152	T13220	T13216	conj	knockout,type
R8153	T13221	T13193	punct	.,is
R8154	T13223	T13224	amod	Hepatic,levels
R8155	T13224	T13225	nsubjpass	levels,reduced
R8156	T13226	T13224	prep	of,levels
R8157	T13227	T13228	npadvmod	GC,measured
R8158	T13228	T13230	amod	measured,cholesterol
R8159	T13229	T13228	punct	-,measured
R8160	T13230	T13226	pobj	cholesterol,of
R8161	T13231	T13225	auxpass	were,reduced
R8162	T13232	T13225	advmod	greatly,reduced
R8163	T13233	T13225	prep	in,reduced
R8164	T13234	T13235	nmod	Abcg8,-
R8165	T13235	T13238	punct	-,mice
R8166	T13236	T13235	punct	-,-
R8167	T13237	T13235	punct	/,-
R8168	T13238	T13233	pobj	mice,in
R8169	T13239	T13225	prep	compared,reduced
R8170	T13240	T13239	prep	to,compared
R8171	T13241	T13242	amod	wild,type
R8172	T13242	T13244	compound	type,mice
R8173	T13243	T13242	punct	-,type
R8174	T13244	T13240	pobj	mice,to
R8175	T13245	T13225	punct	", ",reduced
R8176	T13246	T13225	cc	yet,reduced
R8177	T13247	T13248	compound	mRNA,levels
R8178	T13248	T13249	nsubjpass	levels,reduced
R8179	T13249	T13225	conj	reduced,reduced
R8180	T13250	T13248	prep	of,levels
R8181	T13251	T13252	compound	HMG,CoA
R8182	T13252	T13254	compound	CoA,reductase
R8183	T13253	T13252	punct	-,CoA
R8184	T13254	T13250	pobj	reductase,of
R8185	T13255	T13248	cc	and,levels
R8186	T13256	T13257	compound	enzyme,activity
R8187	T13257	T13248	conj	activity,levels
R8188	T13258	T13249	auxpass	are,reduced
R8189	T13259	T13249	advmod	also,reduced
R8190	T13260	T13249	prep	without,reduced
R8191	T13261	T13262	det	any,change
R8192	T13262	T13260	pobj	change,without
R8193	T13263	T13262	amod	significant,change
R8194	T13264	T13262	prep	in,change
R8195	T13265	T13264	pobj	mRNA,in
R8196	T13266	T13265	prep	of,mRNA
R8197	T13267	T13268	det	the,Srebps
R8198	T13268	T13266	pobj	Srebps,of
R8199	T13269	T13249	punct	.,reduced
R8200	T13271	T13272	det	The,basis
R8201	T13272	T13273	nsubj	basis,is
R8202	T13274	T13272	prep	for,basis
R8203	T13275	T13274	pobj	this,for
R8204	T13276	T13273	neg	not,is
R8205	T13277	T13273	acomp	clear,is
R8206	T13278	T13273	prep	at,is
R8207	T13279	T13278	amod	present,at
R8208	T13280	T13273	punct	", ",is
R8209	T13281	T13282	mark	unless,leads
R8210	T13282	T13273	advcl	leads,is
R8211	T13283	T13284	det	the,increase
R8212	T13284	T13282	nsubj	increase,leads
R8213	T13285	T13284	prep	in,increase
R8214	T13286	T13287	amod	non-plant,sterols
R8215	T13287	T13285	pobj	sterols,in
R8216	T13288	T13282	prep	to,leads
R8217	T13289	T13290	det	a,suppression
R8218	T13290	T13288	pobj	suppression,to
R8219	T13291	T13290	prep	of,suppression
R8220	T13292	T13293	compound	SREBP,activation
R8221	T13293	T13291	pobj	activation,of
R8222	T13294	T13273	punct	.,is
R8223	T13296	T13297	nsubjpass	Sitosterol,shown
R8224	T13298	T13297	aux	has,shown
R8225	T13299	T13297	auxpass	been,shown
R8226	T13300	T13301	aux	to,inhibit
R8227	T13301	T13297	xcomp	inhibit,shown
R8228	T13302	T13301	advmod	directly,inhibit
R8229	T13303	T13304	compound	Cyp7a1,activity
R8230	T13304	T13301	dobj	activity,inhibit
R8231	T13305	T13306	advmod	in,vitro
R8232	T13306	T13301	advmod	vitro,inhibit
R8233	T13307	T13297	cc	and,shown
R8234	T13308	T13309	nsubj	we,presume
R8235	T13309	T13297	conj	presume,shown
R8236	T13310	T13311	nsubj	this,account
R8237	T13311	T13309	advcl	account,presume
R8238	T13312	T13311	aux	may,account
R8239	T13313	T13311	prep	for,account
R8240	T13314	T13315	det	the,activity
R8241	T13315	T13313	pobj	activity,for
R8242	T13316	T13315	amod	reduced,activity
R8243	T13317	T13315	compound	enzyme,activity
R8244	T13318	T13319	punct	[,39
R8245	T13319	T13309	parataxis	39,presume
R8246	T13320	T13319	punct	],39
R8247	T13321	T13309	punct	.,presume
R8248	T13323	T13324	prep	In,increased
R8249	T13325	T13326	amod	preliminary,studies
R8250	T13326	T13323	pobj	studies,In
R8251	T13327	T13324	punct	", ",increased
R8252	T13328	T13329	advmod	when,placed
R8253	T13329	T13324	advcl	placed,increased
R8254	T13330	T13331	compound	knockout,mice
R8255	T13331	T13329	nsubjpass	mice,placed
R8256	T13332	T13329	auxpass	are,placed
R8257	T13333	T13329	prep	on,placed
R8258	T13334	T13335	det	a,diet
R8259	T13335	T13333	pobj	diet,on
R8260	T13336	T13337	amod	low,sitosterol
R8261	T13337	T13335	compound	sitosterol,diet
R8262	T13338	T13324	punct	", ",increased
R8263	T13339	T13340	det	the,activity
R8264	T13340	T13324	nsubjpass	activity,increased
R8265	T13341	T13340	prep	of,activity
R8266	T13342	T13343	det	this,enzyme
R8267	T13343	T13341	pobj	enzyme,of
R8268	T13344	T13340	punct	", ",activity
R8269	T13345	T13346	advmod	as,as
R8270	T13346	T13340	cc	as,activity
R8271	T13347	T13346	advmod	well,as
R8272	T13348	T13349	det	the,levels
R8273	T13349	T13340	conj	levels,activity
R8274	T13350	T13349	compound	mRNA,levels
R8275	T13351	T13324	auxpass	are,increased
R8276	T13352	T13353	punct	(,E
R8277	T13353	T13324	meta	E,increased
R8278	T13354	T13353	nmod	Klett,E
R8279	T13355	T13353	cc	and,E
R8280	T13356	T13353	conj	S,E
R8281	T13357	T13356	nmod	Patel,S
R8282	T13358	T13356	punct	", ",S
R8283	T13359	T13360	amod	unpublished,observations
R8284	T13360	T13356	conj	observations,S
R8285	T13361	T13360	punct	),observations
R8286	T13362	T13324	punct	.,increased
R8287	T13364	T13365	det	Another,prediction
R8288	T13365	T13366	nsubj	prediction,is
R8289	T13367	T13365	punct	", ",prediction
R8290	T13368	T13365	prep	based,prediction
R8291	T13369	T13368	prep	upon,based
R8292	T13370	T13371	det	the,levels
R8293	T13371	T13369	pobj	levels,upon
R8294	T13372	T13371	nmod	enzyme,levels
R8295	T13373	T13372	cc	and,enzyme
R8296	T13374	T13372	conj	mRNA,enzyme
R8297	T13375	T13371	prep	in,levels
R8298	T13376	T13377	compound	knockout,mice
R8299	T13377	T13375	pobj	mice,in
R8300	T13378	T13366	punct	", ",is
R8301	T13379	T13380	mark	that,show
R8302	T13380	T13366	ccomp	show,is
R8303	T13381	T13382	mark	if,placed
R8304	T13382	T13380	advcl	placed,show
R8305	T13383	T13384	det	these,mice
R8306	T13384	T13382	nsubjpass	mice,placed
R8307	T13385	T13382	auxpass	are,placed
R8308	T13386	T13382	prep	on,placed
R8309	T13387	T13388	det	a,diet
R8310	T13388	T13386	pobj	diet,on
R8311	T13389	T13390	amod	high,cholesterol
R8312	T13390	T13388	nmod	cholesterol,diet
R8313	T13391	T13390	punct	/,cholesterol
R8314	T13392	T13393	amod	high,sitosterol
R8315	T13393	T13390	appos	sitosterol,cholesterol
R8316	T13394	T13380	punct	", ",show
R8317	T13395	T13380	nsubj	they,show
R8318	T13396	T13380	aux	may,show
R8319	T13397	T13398	amod	significant,accumulation
R8320	T13398	T13380	dobj	accumulation,show
R8321	T13399	T13398	prep	of,accumulation
R8322	T13400	T13401	det	both,sterols
R8323	T13401	T13399	pobj	sterols,of
R8324	T13402	T13398	prep	in,accumulation
R8325	T13403	T13404	det	the,body
R8326	T13404	T13402	pobj	body,in
R8327	T13405	T13366	punct	.,is
R8328	T13407	T13408	nsubj	This,be
R8329	T13409	T13408	aux	may,be
R8330	T13410	T13408	advmod	also,be
R8331	T13411	T13408	acomp	relevant,be
R8332	T13412	T13411	prep	to,relevant
R8333	T13413	T13414	det	the,disorder
R8334	T13414	T13412	pobj	disorder,to
R8335	T13415	T13414	amod	human,disorder
R8336	T13416	T13417	advmod	where,manifest
R8337	T13417	T13414	relcl	manifest,disorder
R8338	T13418	T13419	det	some,individuals
R8339	T13419	T13417	nsubj	individuals,manifest
R8340	T13420	T13421	punct	", ",all
R8341	T13421	T13419	parataxis	all,individuals
R8342	T13422	T13421	mark	although,all
R8343	T13423	T13421	neg	not,all
R8344	T13424	T13419	amod	affected,individuals
R8345	T13425	T13417	punct	", ",manifest
R8346	T13426	T13427	amod	premature,disease
R8347	T13427	T13417	dobj	disease,manifest
R8348	T13428	T13429	amod	coronary,artery
R8349	T13429	T13427	compound	artery,disease
R8350	T13430	T13408	punct	.,be
R8351	T13432	T13433	compound	Abcg8,sterolin
R8352	T13433	T13435	npadvmod	sterolin,deficient
R8353	T13434	T13433	punct	/,sterolin
R8354	T13435	T13438	amod	deficient,mice
R8355	T13436	T13433	punct	-,sterolin
R8356	T13437	T13433	nummod	2,sterolin
R8357	T13438	T13439	nsubj	mice,allow
R8358	T13440	T13439	advmod	also,allow
R8359	T13441	T13442	nsubj	us,examine
R8360	T13442	T13439	ccomp	examine,allow
R8361	T13443	T13442	aux	to,examine
R8362	T13444	T13445	det	the,role
R8363	T13445	T13442	dobj	role,examine
R8364	T13446	T13445	prep	of,role
R8365	T13447	T13448	det	this,protein
R8366	T13448	T13446	pobj	protein,of
R8367	T13449	T13445	prep	in,role
R8368	T13450	T13451	amod	biliary,sterol
R8369	T13451	T13452	compound	sterol,secretion
R8370	T13452	T13449	pobj	secretion,in
R8371	T13453	T13439	punct	.,allow
R8372	T13455	T13456	det	The,bile
R8373	T13456	T13457	nsubj	bile,is
R8374	T13458	T13459	npadvmod	sterol,poor
R8375	T13459	T13457	acomp	poor,is
R8376	T13460	T13457	cc	and,is
R8377	T13461	T13462	prep	upon,respond
R8378	T13462	T13457	conj	respond,is
R8379	T13463	T13461	pobj	stimulation,upon
R8380	T13464	T13463	prep	by,stimulation
R8381	T13465	T13464	pcomp	increasing,by
R8382	T13466	T13467	compound	bile,salt
R8383	T13467	T13468	compound	salt,excretion
R8384	T13468	T13465	dobj	excretion,increasing
R8385	T13469	T13462	punct	", ",respond
R8386	T13470	T13471	compound	Abcg8,sterolin
R8387	T13471	T13473	npadvmod	sterolin,deficient
R8388	T13472	T13471	punct	/,sterolin
R8389	T13473	T13476	amod	deficient,mice
R8390	T13474	T13471	punct	-,sterolin
R8391	T13475	T13471	nummod	2,sterolin
R8392	T13476	T13462	nsubj	mice,respond
R8393	T13477	T13462	aux	can,respond
R8394	T13478	T13462	neg	not,respond
R8395	T13479	T13462	punct	", ",respond
R8396	T13480	T13462	prep	in,respond
R8397	T13481	T13480	pobj	contrast,in
R8398	T13482	T13481	prep	to,contrast
R8399	T13483	T13484	det	the,mice
R8400	T13484	T13482	pobj	mice,to
R8401	T13485	T13486	amod	wild,type
R8402	T13486	T13484	compound	type,mice
R8403	T13487	T13486	punct	-,type
R8404	T13488	T13462	punct	.,respond
R8405	T13490	T13491	advmod	Thus,is
R8406	T13492	T13491	punct	", ",is
R8407	T13493	T13494	compound	Abcg8,sterolin
R8408	T13494	T13491	nsubj	sterolin,is
R8409	T13495	T13494	punct	/,sterolin
R8410	T13496	T13494	punct	-,sterolin
R8411	T13497	T13494	nummod	2,sterolin
R8412	T13498	T13491	acomp	necessary,is
R8413	T13499	T13498	prep	for,necessary
R8414	T13500	T13501	compound	cholesterol,secretion
R8415	T13501	T13499	pobj	secretion,for
R8416	T13502	T13498	cc	but,necessary
R8417	T13503	T13502	neg	not,but
R8418	T13504	T13498	conj	necessary,necessary
R8419	T13505	T13504	prep	for,necessary
R8420	T13506	T13507	compound	plant,sterol
R8421	T13507	T13508	compound	sterol,secretion
R8422	T13508	T13505	pobj	secretion,for
R8423	T13509	T13491	punct	.,is
R8424	T13511	T13512	nsubjpass	It,argued
R8425	T13513	T13512	aux	could,argued
R8426	T13514	T13512	auxpass	be,argued
R8427	T13515	T13516	mark	that,is
R8428	T13516	T13512	ccomp	is,argued
R8429	T13517	T13518	mark	although,is
R8430	T13518	T13516	advcl	is,is
R8431	T13519	T13520	det	the,output
R8432	T13520	T13518	nsubj	output,is
R8433	T13521	T13522	amod	biliary,sterol
R8434	T13522	T13520	compound	sterol,output
R8435	T13523	T13518	acomp	comparable,is
R8436	T13524	T13523	prep	to,comparable
R8437	T13525	T13524	pobj	that,to
R8438	T13526	T13525	acl	seen,that
R8439	T13527	T13526	prep	in,seen
R8440	T13528	T13529	amod	wild,type
R8441	T13529	T13531	compound	type,mice
R8442	T13530	T13529	punct	-,type
R8443	T13531	T13527	pobj	mice,in
R8444	T13532	T13516	mark	that,is
R8445	T13533	T13516	nsubj	this,is
R8446	T13534	T13535	advmod	inappropriately,low
R8447	T13535	T13516	acomp	low,is
R8448	T13536	T13512	punct	", ",argued
R8449	T13537	T13538	mark	since,are
R8450	T13538	T13512	advcl	are,argued
R8451	T13539	T13540	det	the,pools
R8452	T13540	T13538	nsubj	pools,are
R8453	T13541	T13540	nmod	tissue,pools
R8454	T13542	T13541	cc	and,tissue
R8455	T13543	T13541	conj	plasma,tissue
R8456	T13544	T13540	prep	of,pools
R8457	T13545	T13544	pobj	sitosterol,of
R8458	T13546	T13547	advmod	so,elevated
R8459	T13547	T13538	acomp	elevated,are
R8460	T13548	T13538	prep	in,are
R8461	T13549	T13550	det	the,mice
R8462	T13550	T13548	pobj	mice,in
R8463	T13551	T13550	compound	knockout,mice
R8464	T13552	T13512	punct	.,argued
R8465	T13554	T13555	prep	Despite,suggests
R8466	T13556	T13557	det	this,caveat
R8467	T13557	T13554	pobj	caveat,Despite
R8468	T13558	T13555	punct	", ",suggests
R8469	T13559	T13560	det	the,findings
R8470	T13560	T13555	nsubj	findings,suggests
R8471	T13561	T13560	prep	of,findings
R8472	T13562	T13561	pobj	sitosterol,of
R8473	T13563	T13562	prep	in,sitosterol
R8474	T13564	T13565	amod	significant,quantities
R8475	T13565	T13563	pobj	quantities,in
R8476	T13566	T13562	prep	in,sitosterol
R8477	T13567	T13568	det	the,bile
R8478	T13568	T13566	pobj	bile,in
R8479	T13569	T13570	mark	that,export
R8480	T13570	T13555	ccomp	export,suggests
R8481	T13571	T13570	nsubj	mechanisms,export
R8482	T13572	T13571	amod	other,mechanisms
R8483	T13573	T13572	prep	than,other
R8484	T13574	T13573	prep	via,than
R8485	T13575	T13576	det	the,sterolins
R8486	T13576	T13574	pobj	sterolins,via
R8487	T13577	T13570	aux	can,export
R8488	T13578	T13570	punct	',export
R8489	T13579	T13570	punct	',export
R8490	T13580	T13570	dobj	some,export
R8491	T13581	T13580	prep	of,some
R8492	T13582	T13583	det	these,sterols
R8493	T13583	T13581	pobj	sterols,of
R8494	T13584	T13555	punct	.,suggests
R8495	T13586	T13587	advmod	Interestingly,show
R8496	T13588	T13587	punct	", ",show
R8497	T13589	T13590	mark	although,seem
R8498	T13590	T13587	advcl	seem,show
R8499	T13591	T13590	nsubj	humans,seem
R8500	T13592	T13593	dep	who,are
R8501	T13593	T13591	relcl	are,humans
R8502	T13594	T13593	acomp	heterozygous,are
R8503	T13595	T13594	prep	for,heterozygous
R8504	T13596	T13597	amod	genetic,defects
R8505	T13597	T13595	pobj	defects,for
R8506	T13598	T13597	prep	in,defects
R8507	T13599	T13600	preconj	either,ABCG5
R8508	T13600	T13598	pobj	ABCG5,in
R8509	T13601	T13600	cc	or,ABCG5
R8510	T13602	T13600	conj	ABCG8,ABCG5
R8511	T13603	T13604	aux	to,be
R8512	T13604	T13590	xcomp	be,seem
R8513	T13605	T13606	advmod	phenotypically,normal
R8514	T13606	T13604	acomp	normal,be
R8515	T13607	T13587	punct	", ",show
R8516	T13608	T13587	nsubj	mice,show
R8517	T13609	T13608	amod	heterozygous,mice
R8518	T13610	T13609	prep	for,heterozygous
R8519	T13611	T13612	nmod	Abcg8,sterolin
R8520	T13612	T13614	nmod	sterolin,deficiency
R8521	T13613	T13612	punct	/,sterolin
R8522	T13614	T13610	pobj	deficiency,for
R8523	T13615	T13612	punct	-,sterolin
R8524	T13616	T13612	nummod	2,sterolin
R8525	T13617	T13618	mark	that,is
R8526	T13618	T13587	ccomp	is,show
R8527	T13619	T13620	compound	sterol,secretion
R8528	T13620	T13618	nsubj	secretion,is
R8529	T13621	T13618	acomp	impaired,is
R8530	T13622	T13621	punct	", ",impaired
R8531	T13623	T13621	cc	but,impaired
R8532	T13624	T13623	neg	not,but
R8533	T13625	T13621	conj	absent,impaired
R8534	T13626	T13587	punct	.,show
R8535	T13629	T13630	mark	that,show
R8536	T13630	T13628	ccomp	show,Note
R8537	T13631	T13630	prep	under,show
R8538	T13632	T13633	amod	steady,state
R8539	T13633	T13635	compound	state,conditions
R8540	T13634	T13633	punct	-,state
R8541	T13635	T13631	pobj	conditions,under
R8542	T13636	T13630	prep	on,show
R8543	T13637	T13638	det	a,diet
R8544	T13638	T13636	pobj	diet,on
R8545	T13639	T13640	compound	rodent,chow
R8546	T13640	T13638	compound	chow,diet
R8547	T13641	T13630	punct	", ",show
R8548	T13642	T13643	amod	heterozygous,animals
R8549	T13643	T13630	nsubj	animals,show
R8550	T13644	T13645	det	no,elevations
R8551	T13645	T13630	dobj	elevations,show
R8552	T13646	T13645	amod	significant,elevations
R8553	T13647	T13645	prep	in,elevations
R8554	T13648	T13649	nmod	plasma,sitosterol
R8555	T13649	T13652	compound	sitosterol,levels
R8556	T13650	T13648	cc	or,plasma
R8557	T13651	T13648	conj	tissue,plasma
R8558	T13652	T13647	pobj	levels,in
R8559	T13653	T13630	punct	", ",show
R8560	T13654	T13630	advcl	suggesting,show
R8561	T13655	T13656	mark	that,be
R8562	T13656	T13654	ccomp	be,suggesting
R8563	T13657	T13658	det	the,activity
R8564	T13658	T13656	nsubj	activity,be
R8565	T13659	T13658	prep	of,activity
R8566	T13660	T13661	det	these,proteins
R8567	T13661	T13659	pobj	proteins,of
R8568	T13662	T13656	aux	may,be
R8569	T13663	T13656	neg	not,be
R8570	T13664	T13665	npadvmod	rate,limiting
R8571	T13665	T13656	acomp	limiting,be
R8572	T13666	T13628	punct	.,Note
R8573	T13668	T13669	advmod	Furthermore,showed
R8574	T13670	T13669	punct	", ",showed
R8575	T13671	T13672	det	the,mice
R8576	T13672	T13669	nsubj	mice,showed
R8577	T13673	T13672	amod	heterozygous,mice
R8578	T13674	T13672	nmod	Abcg8,mice
R8579	T13675	T13674	punct	+,Abcg8
R8580	T13676	T13674	punct	/,Abcg8
R8581	T13677	T13674	punct	-,Abcg8
R8582	T13678	T13679	amod	higher,levels
R8583	T13679	T13669	dobj	levels,showed
R8584	T13680	T13679	prep	of,levels
R8585	T13681	T13682	amod	biliary,sitosterol
R8586	T13682	T13680	pobj	sitosterol,of
R8587	T13683	T13679	amod	relative,levels
R8588	T13684	T13683	prep	to,relative
R8589	T13685	T13686	det	the,mice
R8590	T13686	T13684	pobj	mice,to
R8591	T13687	T13688	amod	wild,type
R8592	T13688	T13686	compound	type,mice
R8593	T13689	T13688	punct	-,type
R8594	T13690	T13669	punct	.,showed
R8595	T13692	T13693	mark	While,reduced
R8596	T13693	T13702	advcl	reduced,is
R8597	T13694	T13695	det	the,rate
R8598	T13695	T13693	nsubjpass	rate,reduced
R8599	T13696	T13695	prep	of,rate
R8600	T13697	T13698	amod	total,secretion
R8601	T13698	T13696	pobj	secretion,of
R8602	T13699	T13700	amod	biliary,sterol
R8603	T13700	T13698	compound	sterol,secretion
R8604	T13701	T13693	auxpass	is,reduced
R8605	T13703	T13693	prep	compared,reduced
R8606	T13704	T13703	prep	to,compared
R8607	T13705	T13706	amod	wild,type
R8608	T13706	T13708	compound	type,mice
R8609	T13707	T13706	punct	-,type
R8610	T13708	T13704	pobj	mice,to
R8611	T13709	T13693	punct	", ",reduced
R8612	T13710	T13711	mark	since,are
R8613	T13711	T13693	advcl	are,reduced
R8614	T13712	T13713	det	the,mice
R8615	T13713	T13711	nsubj	mice,are
R8616	T13714	T13713	amod	heterozygous,mice
R8617	T13715	T13711	neg	not,are
R8618	T13716	T13711	acomp	sitosterolemic,are
R8619	T13717	T13702	punct	", ",is
R8620	T13718	T13702	preconj	either,is
R8621	T13719	T13720	det	the,activity
R8622	T13720	T13702	nsubj	activity,is
R8623	T13721	T13720	prep	of,activity
R8624	T13722	T13723	det	these,proteins
R8625	T13723	T13721	pobj	proteins,of
R8626	T13724	T13702	neg	not,is
R8627	T13725	T13726	npadvmod	rate,limiting
R8628	T13726	T13702	acomp	limiting,is
R8629	T13727	T13702	cc	or,is
R8630	T13728	T13729	amod	other,mechanisms
R8631	T13729	T13730	nsubj	mechanisms,compensate
R8632	T13730	T13702	conj	compensate,is
R8633	T13731	T13730	aux	can,compensate
R8634	T13732	T13730	prep	for,compensate
R8635	T13733	T13734	det	a,loss
R8636	T13734	T13732	pobj	loss,for
R8637	T13735	T13736	nummod	50,%
R8638	T13736	T13734	compound	%,loss
R8639	T13737	T13734	prep	of,loss
R8640	T13738	T13737	pobj	activity,of
R8641	T13739	T13730	punct	.,compensate
R8642	T13741	T13742	prep	On,negate
R8643	T13743	T13744	det	the,hand
R8644	T13744	T13741	pobj	hand,On
R8645	T13745	T13744	amod	other,hand
R8646	T13746	T13742	punct	", ",negate
R8647	T13747	T13748	mark	since,is
R8648	T13748	T13742	advcl	is,negate
R8649	T13749	T13748	nsubj	feeding,is
R8650	T13750	T13748	acomp	intermittent,is
R8651	T13751	T13742	punct	", ",negate
R8652	T13752	T13753	det	a,secretion
R8653	T13753	T13742	nsubj	secretion,negate
R8654	T13754	T13753	amod	slow,secretion
R8655	T13755	T13754	punct	", ",slow
R8656	T13756	T13754	cc	but,slow
R8657	T13757	T13754	conj	continuous,slow
R8658	T13758	T13753	prep	during,secretion
R8659	T13759	T13760	amod	post-prandial,periods
R8660	T13760	T13758	pobj	periods,during
R8661	T13761	T13742	aux	could,negate
R8662	T13762	T13742	advmod	easily,negate
R8663	T13763	T13764	det	any,increase
R8664	T13764	T13742	dobj	increase,negate
R8665	T13765	T13764	amod	mild,increase
R8666	T13766	T13764	amod	temporary,increase
R8667	T13767	T13764	prep	in,increase
R8668	T13768	T13769	nmod	tissue,levels
R8669	T13769	T13767	pobj	levels,in
R8670	T13770	T13768	cc	and,tissue
R8671	T13771	T13768	conj	plasma,tissue
R8672	T13772	T13773	compound	plant,sterol
R8673	T13773	T13769	compound	sterol,levels
R8674	T13774	T13764	prep	in,increase
R8675	T13775	T13776	amod	heterozygous,animals
R8676	T13776	T13774	pobj	animals,in
R8677	T13777	T13742	punct	.,negate
R8678	T13779	T13780	nsubj	This,be
R8679	T13781	T13780	aux	may,be
R8680	T13782	T13780	acomp	amenable,be
R8681	T13783	T13782	prep	to,amenable
R8682	T13784	T13783	pcomp	testing,to
R8683	T13785	T13784	prep	by,testing
R8684	T13786	T13785	pcomp	placing,by
R8685	T13787	T13788	det	these,animals
R8686	T13788	T13786	dobj	animals,placing
R8687	T13789	T13786	prep	on,placing
R8688	T13790	T13791	det	a,diet
R8689	T13791	T13789	pobj	diet,on
R8690	T13792	T13793	amod	high,sterol
R8691	T13793	T13791	compound	sterol,diet
R8692	T13794	T13793	compound	plant,sterol
R8693	T13795	T13786	cc	and,placing
R8694	T13796	T13786	conj	measuring,placing
R8695	T13797	T13798	det	the,levels
R8696	T13798	T13796	dobj	levels,measuring
R8697	T13799	T13800	compound	plasma,sterol
R8698	T13800	T13798	compound	sterol,levels
R8699	T13801	T13780	punct	.,be
R8700	T13803	T13804	advmod	Recently,reported
R8701	T13805	T13804	punct	", ",reported
R8702	T13806	T13804	nsubj	Kosters,reported
R8703	T13807	T13808	advmod	et,al.
R8704	T13808	T13806	advmod	al.,Kosters
R8705	T13809	T13810	mark	that,correlated
R8706	T13810	T13804	ccomp	correlated,reported
R8707	T13811	T13812	compound	Abcg5,Abcg8
R8708	T13812	T13814	compound	Abcg8,mRNA
R8709	T13813	T13812	punct	/,Abcg8
R8710	T13814	T13815	compound	mRNA,expression
R8711	T13815	T13810	nsubj	expression,correlated
R8712	T13816	T13815	prep	in,expression
R8713	T13817	T13818	det	a,variety
R8714	T13818	T13816	pobj	variety,in
R8715	T13819	T13818	prep	of,variety
R8716	T13820	T13821	amod	different,strains
R8717	T13821	T13819	pobj	strains,of
R8718	T13822	T13821	compound	mouse,strains
R8719	T13823	T13810	prep	with,correlated
R8720	T13824	T13825	amod	biliary,cholesterol
R8721	T13825	T13826	compound	cholesterol,secretion
R8722	T13826	T13827	compound	secretion,rates
R8723	T13827	T13823	pobj	rates,with
R8724	T13828	T13829	punct	[,30
R8725	T13829	T13804	parataxis	30,reported
R8726	T13830	T13829	punct	],30
R8727	T13831	T13804	punct	.,reported
R8728	T13833	T13834	prep	In,altered
R8729	T13835	T13836	poss	their,studies
R8730	T13836	T13833	pobj	studies,In
R8731	T13837	T13834	punct	", ",altered
R8732	T13838	T13839	mark	although,showed
R8733	T13839	T13834	advcl	showed,altered
R8734	T13840	T13841	npadvmod	diosgenin,fed
R8735	T13841	T13843	amod	fed,mice
R8736	T13842	T13841	punct	-,fed
R8737	T13843	T13839	nsubj	mice,showed
R8738	T13844	T13845	det	a,increase
R8739	T13845	T13839	dobj	increase,showed
R8740	T13846	T13845	amod	marked,increase
R8741	T13847	T13845	prep	in,increase
R8742	T13848	T13849	amod	biliary,output
R8743	T13849	T13847	pobj	output,in
R8744	T13850	T13849	compound	cholesterol,output
R8745	T13851	T13834	punct	", ",altered
R8746	T13852	T13853	compound	mRNA,levels
R8747	T13853	T13834	nsubjpass	levels,altered
R8748	T13854	T13853	prep	of,levels
R8749	T13855	T13854	pobj	Abcg5,of
R8750	T13856	T13855	cc	and,Abcg5
R8751	T13857	T13855	conj	Abcg8,Abcg5
R8752	T13858	T13834	auxpass	were,altered
R8753	T13859	T13834	neg	not,altered
R8754	T13860	T13834	punct	.,altered
R8755	T13862	T13863	advcl	Using,altered
R8756	T13864	T13865	compound	Western,blot
R8757	T13865	T13866	compound	blot,analyses
R8758	T13866	T13862	dobj	analyses,Using
R8759	T13867	T13863	punct	", ",altered
R8760	T13868	T13869	det	the,level
R8761	T13869	T13863	nsubjpass	level,altered
R8762	T13870	T13869	compound	protein,level
R8763	T13871	T13869	prep	of,level
R8764	T13872	T13871	pobj	Abcg5,of
R8765	T13873	T13863	auxpass	was,altered
R8766	T13874	T13863	advmod	also,altered
R8767	T13875	T13863	neg	not,altered
R8768	T13876	T13863	prep	by,altered
R8769	T13877	T13876	pobj	diosgenin,by
R8770	T13878	T13863	punct	", ",altered
R8771	T13879	T13880	mark	although,measured
R8772	T13880	T13863	advcl	measured,altered
R8773	T13881	T13880	nsubjpass	Abcg8,measured
R8774	T13882	T13880	auxpass	was,measured
R8775	T13883	T13880	neg	not,measured
R8776	T13884	T13863	punct	.,altered
R8777	T13886	T13887	nsubj	They,concluded
R8778	T13888	T13889	mark	that,be
R8779	T13889	T13887	ccomp	be,concluded
R8780	T13890	T13891	det	a,route
R8781	T13891	T13889	nsubj	route,be
R8782	T13892	T13891	amod	parallel,route
R8783	T13893	T13891	prep	for,route
R8784	T13894	T13895	amod	biliary,cholesterol
R8785	T13895	T13896	compound	cholesterol,secretion
R8786	T13896	T13893	pobj	secretion,for
R8787	T13897	T13889	aux	might,be
R8788	T13898	T13889	acomp	operational,be
R8789	T13899	T13889	punct	", ",be
R8790	T13900	T13889	advcl	independent,be
R8791	T13901	T13900	prep	of,independent
R8792	T13902	T13903	det	the,sterolins
R8793	T13903	T13901	pobj	sterolins,of
R8794	T13904	T13887	punct	.,concluded
R8795	T13906	T13907	mark	While,is
R8796	T13907	T13911	advcl	is,appear
R8797	T13908	T13909	amod	biliary,cholesterol
R8798	T13909	T13910	compound	cholesterol,secretion
R8799	T13910	T13907	nsubj	secretion,is
R8800	T13912	T13907	neg	not,is
R8801	T13913	T13914	advmod	completely,absent
R8802	T13914	T13907	acomp	absent,is
R8803	T13915	T13907	prep	in,is
R8804	T13916	T13917	det	the,mice
R8805	T13917	T13915	pobj	mice,in
R8806	T13918	T13919	npadvmod	Abcg8,deficient
R8807	T13919	T13917	amod	deficient,mice
R8808	T13920	T13919	punct	-,deficient
R8809	T13921	T13911	nsubj	it,appear
R8810	T13922	T13911	aux	would,appear
R8811	T13923	T13924	mark	that,plays
R8812	T13924	T13911	ccomp	plays,appear
R8813	T13925	T13924	nsubj	sterolin,plays
R8814	T13926	T13925	punct	-,sterolin
R8815	T13927	T13925	nummod	2,sterolin
R8816	T13928	T13929	det	a,role
R8817	T13929	T13924	dobj	role,plays
R8818	T13930	T13929	amod	major,role
R8819	T13931	T13924	prep	in,plays
R8820	T13932	T13933	det	this,process
R8821	T13933	T13931	pobj	process,in
R8822	T13934	T13911	punct	.,appear
R8823	T13936	T13937	prep	In,identified
R8824	T13938	T13939	det	a,screen
R8825	T13939	T13936	pobj	screen,In
R8826	T13940	T13939	amod	genetic,screen
R8827	T13941	T13939	prep	of,screen
R8828	T13942	T13943	compound	plasma,levels
R8829	T13943	T13941	pobj	levels,of
R8830	T13944	T13945	compound	plant,sterol
R8831	T13945	T13943	compound	sterol,levels
R8832	T13946	T13937	punct	", ",identified
R8833	T13947	T13937	nsubj	Sehayek,identified
R8834	T13948	T13949	advmod	et,al.
R8835	T13949	T13947	advmod	al.,Sehayek
R8836	T13950	T13951	nummod	three,loci
R8837	T13951	T13937	dobj	loci,identified
R8838	T13952	T13953	dep	that,be
R8839	T13953	T13951	relcl	be,loci
R8840	T13954	T13953	aux	may,be
R8841	T13955	T13953	acomp	responsible,be
R8842	T13956	T13955	prep	for,responsible
R8843	T13957	T13956	pcomp	controlling,for
R8844	T13958	T13959	compound	plasma,levels
R8845	T13959	T13957	dobj	levels,controlling
R8846	T13960	T13961	compound	plant,sterol
R8847	T13961	T13959	compound	sterol,levels
R8848	T13962	T13937	cc	and,identified
R8849	T13963	T13964	neg	not,one
R8850	T13964	T13965	nsubj	one,mapped
R8851	T13965	T13937	conj	mapped,identified
R8852	T13966	T13964	prep	of,one
R8853	T13967	T13968	det	these,loci
R8854	T13968	T13966	pobj	loci,of
R8855	T13969	T13965	prep	to,mapped
R8856	T13970	T13971	det	the,region
R8857	T13971	T13969	pobj	region,to
R8858	T13972	T13971	amod	murine,region
R8859	T13973	T13971	compound	STSL,region
R8860	T13974	T13975	punct	[,43
R8861	T13975	T13937	parataxis	43,identified
R8862	T13976	T13975	punct	],43
R8863	T13977	T13937	punct	.,identified
R8864	T13979	T13980	advmod	Thus,is
R8865	T13981	T13980	punct	", ",is
R8866	T13982	T13980	expl	there,is
R8867	T13983	T13980	attr	support,is
R8868	T13984	T13983	prep	for,support
R8869	T13985	T13986	advmod	at,four
R8870	T13986	T13988	nummod	four,loci
R8871	T13987	T13986	advmod	least,four
R8872	T13988	T13984	pobj	loci,for
R8873	T13989	T13990	dep	that,involved
R8874	T13990	T13988	relcl	involved,loci
R8875	T13991	T13990	aux	may,involved
R8876	T13992	T13990	auxpass	be,involved
R8877	T13993	T13990	prep	in,involved
R8878	T13994	T13993	pcomp	regulating,in
R8879	T13995	T13996	compound	plasma,levels
R8880	T13996	T13994	dobj	levels,regulating
R8881	T13997	T13998	compound	plant,sterol
R8882	T13998	T13996	compound	sterol,levels
R8883	T13999	T13980	punct	.,is
R8884	T14001	T14002	prep	To,is
R8885	T14003	T14001	pobj	date,To
R8886	T14004	T14005	det	the,locus
R8887	T14005	T14002	nsubj	locus,is
R8888	T14006	T14005	compound	STSL,locus
R8889	T14007	T14008	det	the,one
R8890	T14008	T14002	attr	one,is
R8891	T14009	T14008	amod	only,one
R8892	T14010	T14008	acl	proven,one
R8893	T14011	T14012	aux	to,involved
R8894	T14012	T14010	xcomp	involved,proven
R8895	T14013	T14012	auxpass	be,involved
R8896	T14014	T14012	prep	in,involved
R8897	T14015	T14016	amod	dietary,sterol
R8898	T14016	T14017	compound	sterol,trafficking
R8899	T14017	T14014	pobj	trafficking,in
R8900	T14018	T14002	cc	and,is
R8901	T14019	T14020	det	the,identity
R8902	T14020	T14021	nsubj	identity,remains
R8903	T14021	T14002	conj	remains,is
R8904	T14022	T14020	prep	of,identity
R8905	T14023	T14024	det	the,others
R8906	T14024	T14022	pobj	others,of
R8907	T14025	T14026	aux	to,elucidated
R8908	T14026	T14021	xcomp	elucidated,remains
R8909	T14027	T14026	auxpass	be,elucidated
R8910	T14028	T14002	punct	.,is
R8911	T14030	T14031	nsubj	We,proposed
R8912	T14032	T14030	punct	", ",We
R8913	T14033	T14034	advmod	as,as
R8914	T14034	T14030	cc	as,We
R8915	T14035	T14034	advmod	well,as
R8916	T14036	T14030	conj	others,We
R8917	T14037	T14031	punct	", ",proposed
R8918	T14038	T14031	aux	have,proposed
R8919	T14039	T14040	compound	Abcg5,sterolin
R8920	T14040	T14031	dobj	sterolin,proposed
R8921	T14041	T14040	punct	/,sterolin
R8922	T14042	T14040	punct	-,sterolin
R8923	T14043	T14040	nummod	1,sterolin
R8924	T14044	T14040	cc	and,sterolin
R8925	T14045	T14046	compound	Abcg8,sterolin
R8926	T14046	T14040	conj	sterolin,sterolin
R8927	T14047	T14046	punct	/,sterolin
R8928	T14048	T14046	punct	-,sterolin
R8929	T14049	T14046	nummod	2,sterolin
R8930	T14050	T14051	aux	to,function
R8931	T14051	T14031	xcomp	function,proposed
R8932	T14052	T14051	prep	as,function
R8933	T14053	T14054	amod	obligate,heterodimers
R8934	T14054	T14052	pobj	heterodimers,as
R8935	T14055	T14031	punct	.,proposed
R8936	T14057	T14058	advmod	Thus,is
R8937	T14059	T14058	csubj	knocking,is
R8938	T14060	T14059	prt	out,knocking
R8939	T14061	T14062	compound	Abcg8,sterolin
R8940	T14062	T14059	dobj	sterolin,knocking
R8941	T14063	T14062	punct	/,sterolin
R8942	T14064	T14062	punct	-,sterolin
R8943	T14065	T14062	nummod	2,sterolin
R8944	T14066	T14062	advmod	alone,sterolin
R8945	T14067	T14058	acomp	equivalent,is
R8946	T14068	T14067	prep	to,equivalent
R8947	T14069	T14070	det	a,loss
R8948	T14070	T14068	pobj	loss,to
R8949	T14071	T14070	amod	functional,loss
R8950	T14072	T14070	prep	of,loss
R8951	T14073	T14074	det	both,sterolins
R8952	T14074	T14072	pobj	sterolins,of
R8953	T14075	T14058	punct	.,is
R8954	T14077	T14078	nsubjpass	It,shown
R8955	T14079	T14078	aux	has,shown
R8956	T14080	T14078	auxpass	been,shown
R8957	T14081	T14082	mark	that,required
R8958	T14082	T14078	ccomp	required,shown
R8959	T14083	T14084	nmod	Abcg8,sterolin
R8960	T14084	T14086	nmod	sterolin,expression
R8961	T14085	T14084	punct	/,sterolin
R8962	T14086	T14082	nsubjpass	expression,required
R8963	T14087	T14084	punct	-,sterolin
R8964	T14088	T14084	nummod	2,sterolin
R8965	T14089	T14082	auxpass	is,required
R8966	T14090	T14082	prep	for,required
R8967	T14091	T14092	amod	correct,trafficking
R8968	T14092	T14090	pobj	trafficking,for
R8969	T14093	T14092	amod	apical,trafficking
R8970	T14094	T14092	prep	of,trafficking
R8971	T14095	T14096	compound	Abcg5,sterolin
R8972	T14096	T14094	pobj	sterolin,of
R8973	T14097	T14096	punct	/,sterolin
R8974	T14098	T14096	punct	-,sterolin
R8975	T14099	T14096	nummod	1,sterolin
R8976	T14100	T14092	prep	to,trafficking
R8977	T14101	T14102	det	the,surface
R8978	T14102	T14100	pobj	surface,to
R8979	T14103	T14102	amod	apical,surface
R8980	T14104	T14092	prep	in,trafficking
R8981	T14105	T14106	det	a,line
R8982	T14106	T14104	pobj	line,in
R8983	T14107	T14108	amod	polarized,cell
R8984	T14108	T14106	compound	cell,line
R8985	T14109	T14104	cc	and,in
R8986	T14110	T14111	advmod	more,recently
R8987	T14111	T14112	advmod	recently,vivo
R8988	T14112	T14104	conj	vivo,in
R8989	T14113	T14112	advmod	in,vivo
R8990	T14186	T14184	punct	/,-
R8991	T14114	T14092	prep	by,trafficking
R8992	T14187	T14173	punct	", ",exit
R8993	T14115	T14116	compound	over-expression,experiments
R8994	T14188	T14173	aux	does,exit
R8995	T14189	T14173	neg	not,exit
R8996	T14190	T14191	det	the,reticulum
R8997	T14116	T14114	pobj	experiments,by
R8998	T14191	T14173	dobj	reticulum,exit
R8999	T14192	T14191	amod	endoplasmic,reticulum
R9000	T14193	T14163	punct	.,appear
R9001	T14195	T14196	advmod	However,appears
R9002	T14117	T14118	punct	[,19
R9003	T14197	T14196	punct	", ",appears
R9004	T14198	T14196	prep	by,appears
R9005	T14118	T14078	parataxis	19,shown
R9006	T14199	T14198	pobj	immunohistochemistry,by
R9007	T14119	T14118	nummod	18,19
R9008	T14120	T14118	punct	",",19
R9009	T14200	T14196	nsubj	it,appears
R9010	T14201	T14202	mark	that,expressed
R9011	T14121	T14118	punct	],19
R9012	T14202	T14196	ccomp	expressed,appears
R9013	T14203	T14204	compound	Abcg5,sterolin
R9014	T14122	T14078	punct	.,shown
R9015	T14204	T14202	nsubjpass	sterolin,expressed
R9016	T14205	T14204	punct	/,sterolin
R9017	T14206	T14204	punct	-,sterolin
R9018	T14124	T14125	prep	In,obtained
R9019	T14207	T14204	nummod	1,sterolin
R9020	T14208	T14202	auxpass	is,expressed
R9021	T14209	T14202	advmod	apically,expressed
R9022	T14126	T14124	pobj	experiments,In
R9023	T14210	T14202	prep	in,expressed
R9024	T14211	T14212	det	the,mice
R9025	T14212	T14210	pobj	mice,in
R9026	T14213	T14214	npadvmod	Abcg8,deficient
R9027	T14127	T14126	acl	shown,experiments
R9028	T14214	T14212	amod	deficient,mice
R9029	T14215	T14214	punct	-,deficient
R9030	T14128	T14127	advmod	here,shown
R9031	T14216	T14202	advmod	regardless,expressed
R9032	T14217	T14216	prep	of,regardless
R9033	T14129	T14125	punct	", ",obtained
R9034	T14218	T14219	det	the,antibody
R9035	T14219	T14217	pobj	antibody,of
R9036	T14220	T14221	compound	anti-Abcg5,sterolin
R9037	T14221	T14219	amod	sterolin,antibody
R9038	T14130	T14125	advcl	using,obtained
R9039	T14222	T14221	punct	/,sterolin
R9040	T14223	T14221	punct	-,sterolin
R9041	T14224	T14221	nummod	1,sterolin
R9042	T14131	T14132	nummod	three,antibodies
R9043	T14225	T14219	acl	used,antibody
R9044	T14226	T14196	punct	.,appears
R9045	T14132	T14130	dobj	antibodies,using
R9046	T14228	T14229	advmod	Interestingly,found
R9047	T14133	T14134	advmod	separately,developed
R9048	T14230	T14229	punct	", ",found
R9049	T14231	T14229	prep	in,found
R9050	T14232	T14233	det	a,report
R9051	T14233	T14231	pobj	report,in
R9052	T14234	T14233	prep	of,report
R9053	T14235	T14236	compound	Abcg5,Abcg8
R9054	T14134	T14132	amod	developed,antibodies
R9055	T14236	T14238	compound	Abcg8,localization
R9056	T14237	T14236	punct	/,Abcg8
R9057	T14238	T14234	pobj	localization,of
R9058	T14135	T14136	compound	anti-Abcg5,sterolin
R9059	T14239	T14238	prep	in,localization
R9060	T14240	T14241	amod	canine,gallbladder
R9061	T14241	T14242	nmod	gallbladder,cells
R9062	T14136	T14132	amod	sterolin,antibodies
R9063	T14242	T14239	pobj	cells,in
R9064	T14243	T14242	amod	epithelial,cells
R9065	T14137	T14136	punct	/,sterolin
R9066	T14244	T14229	punct	", ",found
R9067	T14245	T14246	det	these,proteins
R9068	T14246	T14229	nsubjpass	proteins,found
R9069	T14138	T14136	punct	-,sterolin
R9070	T14247	T14246	nummod	two,proteins
R9071	T14248	T14229	auxpass	were,found
R9072	T14249	T14229	advmod	intracellularly,found
R9073	T14139	T14136	nummod	1,sterolin
R9074	T14250	T14229	prep	under,found
R9075	T14251	T14252	compound	baseline,conditions
R9076	T14252	T14250	pobj	conditions,under
R9077	T14140	T14125	punct	", ",obtained
R9078	T14253	T14254	punct	[,44
R9079	T14254	T14229	parataxis	44,found
R9080	T14141	T14125	nsubj	we,obtained
R9081	T14255	T14254	punct	],44
R9082	T14142	T14125	aux	have,obtained
R9083	T14256	T14229	punct	.,found
R9084	T14143	T14144	amod	inconsistent,data
R9085	T14258	T14259	cc	But,appeared
R9086	T14144	T14125	dobj	data,obtained
R9087	T14260	T14261	advmod	when,given
R9088	T14145	T14144	prep	regarding,data
R9089	T14261	T14259	advcl	given,appeared
R9090	T14262	T14263	nmod	liver,alpha
R9091	T14263	T14266	nmod	alpha,agonist
R9092	T14146	T14147	det	the,trafficking
R9093	T14264	T14263	nmod	X,alpha
R9094	T14265	T14263	nmod	receptor,alpha
R9095	T14147	T14145	pobj	trafficking,regarding
R9096	T14266	T14261	dobj	agonist,given
R9097	T14267	T14263	punct	(,alpha
R9098	T14148	T14147	prep	of,trafficking
R9099	T14268	T14263	appos	LXRα,alpha
R9100	T14269	T14266	punct	),agonist
R9101	T14270	T14259	punct	", ",appeared
R9102	T14271	T14272	compound	Abcg5,sterolin
R9103	T14272	T14259	nsubj	sterolin,appeared
R9104	T14273	T14272	punct	/,sterolin
R9105	T14149	T14150	compound	Abcg5,sterolin
R9106	T14274	T14272	punct	-,sterolin
R9107	T14275	T14272	nummod	1,sterolin
R9108	T14276	T14272	cc	and,sterolin
R9109	T14150	T14148	pobj	sterolin,of
R9110	T14277	T14278	compound	Abcg8,sterolin
R9111	T14278	T14272	conj	sterolin,sterolin
R9112	T14279	T14278	punct	/,sterolin
R9113	T14151	T14150	punct	/,sterolin
R9114	T14280	T14278	punct	-,sterolin
R9115	T14281	T14278	nummod	2,sterolin
R9116	T14282	T14283	aux	to,expressed
R9117	T14152	T14150	punct	-,sterolin
R9118	T14283	T14259	xcomp	expressed,appeared
R9119	T14284	T14283	auxpass	be,expressed
R9120	T14153	T14150	nummod	1,sterolin
R9121	T14285	T14283	prep	at,expressed
R9122	T14286	T14287	det	the,surface
R9123	T14287	T14285	pobj	surface,at
R9124	T14154	T14147	prep	in,trafficking
R9125	T14288	T14287	amod	apical,surface
R9126	T14289	T14259	punct	.,appeared
R9127	T14155	T14156	nmod	Abcg8,mice
R9128	T14156	T14154	pobj	mice,in
R9129	T14157	T14155	punct	-,Abcg8
R9130	T14158	T14155	punct	/,Abcg8
R9131	T14159	T14155	punct	-,Abcg8
R9132	T14291	T14292	prep	In,used
R9133	T14160	T14125	punct	.,obtained
R9134	T14293	T14294	det	these,studies
R9135	T14294	T14291	pobj	studies,In
R9136	T14295	T14296	det	the,antibody
R9137	T14162	T14163	prep	Based,appear
R9138	T14296	T14292	nsubjpass	antibody,used
R9139	T14297	T14296	nmod	UTSW,antibody
R9140	T14298	T14299	compound	anti-Abcg5,sterolin
R9141	T14164	T14162	prep	upon,Based
R9142	T14299	T14296	amod	sterolin,antibody
R9143	T14300	T14299	punct	/,sterolin
R9146	T14165	T14166	amod	classical,maturation
R9149	T14166	T14164	pobj	maturation,upon
R9150	T14306	T14307	nsubj	It,is
R9151	T14167	T14168	compound	N,glycosylation
R9152	T14308	T14307	acomp	apparent,is
R9153	T14309	T14307	prep	with,is
R9154	T14310	T14311	det	these,data
R9155	T14311	T14309	pobj	data,with
R9156	T14312	T14311	amod	conflicting,data
R9157	T14168	T14166	compound	glycosylation,maturation
R9158	T14313	T14314	mark	that,is
R9159	T14314	T14307	ccomp	is,is
R9160	T14315	T14316	det	the,trafficking
R9161	T14316	T14314	nsubj	trafficking,is
R9162	T14169	T14168	punct	-,glycosylation
R9163	T14317	T14316	prep	of,trafficking
R9164	T14318	T14319	det	these,transporters
R9165	T14170	T14163	nsubj	it,appear
R9166	T14319	T14317	pobj	transporters,of
R9167	T14320	T14314	neg	not,is
R9168	T14321	T14322	advmod	as,cut
R9169	T14171	T14163	aux	would,appear
R9170	T14322	T14314	acomp	cut,is
R9171	T14323	T14322	amod	clear,cut
R9172	T14324	T14322	punct	-,cut
R9173	T14172	T14173	mark	that,exit
R9174	T14325	T14307	punct	.,is
R9175	T14327	T14328	prep	In,showed
R9176	T14173	T14163	ccomp	exit,appear
R9177	T14329	T14330	det	a,paper
R9178	T14174	T14175	compound	Abcg5,sterolin
R9179	T14330	T14327	pobj	paper,In
R9180	T14331	T14330	amod	recent,paper
R9181	T14175	T14173	nsubj	sterolin,exit
R9182	T14332	T14328	punct	", ",showed
R9183	T14333	T14328	nsubj	Mangrivite,showed
R9184	T14176	T14175	punct	/,sterolin
R9185	T14334	T14335	advmod	et,al.
R9186	T14335	T14333	advmod	al.,Mangrivite
R9187	T14336	T14337	mark	that,was
R9188	T14177	T14175	punct	-,sterolin
R9189	T14178	T14175	nummod	1,sterolin
R9190	T14179	T14175	punct	", ",sterolin
R9191	T14337	T14328	ccomp	was,showed
R9192	T14338	T14339	det	the,sorting
R9193	T14180	T14175	prep	in,sterolin
R9194	T14339	T14337	nsubj	sorting,was
R9195	T14340	T14339	amod	apical,sorting
R9196	T14341	T14339	prep	of,sorting
R9197	T14181	T14182	det	the,mice
R9198	T14342	T14343	compound	rat,SPNT
R9199	T14343	T14341	pobj	SPNT,of
R9200	T14182	T14180	pobj	mice,in
R9201	T14344	T14339	prep	in,sorting
R9202	T14345	T14346	amod	polarized,cells
R9203	T14183	T14184	nmod	Abcg8,-
R9204	T14346	T14344	pobj	cells,in
R9205	T14347	T14346	amod	renal,cells
R9206	T14348	T14346	amod	epithelial,cells
R9207	T14349	T14337	acomp	independent,was
R9208	T14350	T14349	prep	of,independent
R9209	T14351	T14352	compound	N,glycosylation
R9210	T14352	T14350	pobj	glycosylation,of
R9211	T14184	T14182	punct	-,mice
R9212	T14353	T14352	punct	-,glycosylation
R9213	T14354	T14355	punct	[,45
R9214	T14185	T14184	punct	-,-
R9215	T14355	T14328	parataxis	45,showed
R9216	T14356	T14355	punct	],45
R9217	T14357	T14328	punct	.,showed
R9218	T14397	T14398	nsubj	We,have
R9219	T14359	T14360	advmod	Therefore,exclude
R9220	T14361	T14360	punct	", ",exclude
R9221	T14362	T14360	prep	at,exclude
R9222	T14363	T14364	det	this,time
R9223	T14399	T14400	det	no,assay
R9224	T14364	T14362	pobj	time,at
R9225	T14365	T14360	punct	", ",exclude
R9226	T14366	T14360	nsubj	we,exclude
R9227	T14367	T14360	aux	can,exclude
R9228	T14368	T14360	neg	neither,exclude
R9229	T14400	T14398	dobj	assay,have
R9230	T14369	T14370	det	the,possibility
R9231	T14370	T14360	dobj	possibility,exclude
R9232	T14401	T14400	amod	functional,assay
R9233	T14371	T14372	mark	that,is
R9234	T14372	T14370	acl	is,possibility
R9235	T14373	T14374	nmod	Abcg5,sterolin
R9236	T14402	T14400	prep	for,assay
R9237	T14374	T14376	nmod	sterolin,expression
R9238	T14375	T14374	punct	/,sterolin
R9239	T14376	T14372	nsubj	expression,is
R9240	T14403	T14404	det	these,proteins
R9241	T14377	T14374	punct	-,sterolin
R9242	T14378	T14374	nummod	1,sterolin
R9243	T14379	T14372	acomp	functional,is
R9244	T14404	T14402	pobj	proteins,for
R9245	T14380	T14372	prep	in,is
R9246	T14381	T14382	poss	our,mice
R9247	T14382	T14380	pobj	mice,in
R9248	T14405	T14398	prep	at,have
R9249	T14383	T14384	compound	Abcg8,sterolin
R9250	T14384	T14386	npadvmod	sterolin,deficient
R9251	T14385	T14384	punct	/,sterolin
R9252	T14406	T14405	amod	present,at
R9253	T14386	T14382	amod	deficient,mice
R9254	T14407	T14398	punct	.,have
R9255	T14387	T14384	punct	-,sterolin
R9256	T14388	T14384	nummod	2,sterolin
R9257	T14389	T14360	cc	nor,exclude
R9258	T14390	T14391	aux	can,rule
R9259	T14391	T14360	conj	rule,exclude
R9260	T14392	T14391	nsubj	we,rule
R9261	T14409	T14410	advmod	More,extensive
R9262	T14393	T14391	dobj	it,rule
R9263	T14394	T14391	prt	out,rule
R9264	T14395	T14360	punct	.,exclude
R9265	T14410	T14411	amod	extensive,experiments
R9266	T14411	T14413	nsubj	experiments,are
R9267	T14502	T14503	nsubjpass	It,argued
R9268	T14412	T14411	compound	fractionation,experiments
R9269	T14504	T14503	aux	has,argued
R9270	T14505	T14503	auxpass	been,argued
R9271	T14414	T14413	acomp	underway,are
R9272	T14506	T14507	mark	that,are
R9273	T14415	T14416	aux	to,determine
R9274	T14507	T14503	ccomp	are,argued
R9275	T14508	T14509	compound	Abcg5,sterolin
R9276	T14416	T14413	xcomp	determine,are
R9277	T14509	T14507	nsubj	sterolin,are
R9278	T14510	T14509	punct	/,sterolin
R9279	T14511	T14509	punct	-,sterolin
R9280	T14417	T14416	advmod	better,determine
R9281	T14512	T14509	nummod	1,sterolin
R9282	T14513	T14509	cc	and,sterolin
R9283	T14514	T14515	compound	Abcg8,sterolin
R9284	T14418	T14419	det	the,location
R9285	T14515	T14509	conj	sterolin,sterolin
R9286	T14516	T14515	punct	/,sterolin
R9287	T14517	T14515	punct	-,sterolin
R9288	T14419	T14416	dobj	location,determine
R9289	T14518	T14515	nummod	2,sterolin
R9290	T14519	T14507	acomp	dependent,are
R9291	T14520	T14519	prep	upon,dependent
R9292	T14420	T14419	amod	exact,location
R9293	T14521	T14522	det	each,other
R9294	T14522	T14520	pobj	other,upon
R9295	T14523	T14507	prep	for,are
R9296	T14421	T14419	amod	compartmental,location
R9297	T14524	T14523	pobj	secretion,for
R9298	T14525	T14524	prep	of,secretion
R9299	T14422	T14419	prep	of,location
R9300	T14526	T14527	amod	hepatic,sterols
R9301	T14527	T14525	pobj	sterols,of
R9302	T14528	T14524	prep	into,secretion
R9303	T14423	T14424	compound	Abcg5,sterolin
R9304	T14529	T14528	pobj	bile,into
R9305	T14530	T14531	punct	[,19
R9306	T14531	T14503	parataxis	19,argued
R9307	T14424	T14422	pobj	sterolin,of
R9308	T14532	T14531	punct	],19
R9309	T14533	T14503	punct	.,argued
R9310	T14535	T14536	prep	Given,appear
R9311	T14425	T14424	punct	/,sterolin
R9312	T14537	T14538	det	the,data
R9313	T14538	T14535	pobj	data,Given
R9314	T14539	T14538	acl	presented,data
R9315	T14426	T14424	punct	-,sterolin
R9316	T14540	T14539	advmod	here,presented
R9317	T14541	T14535	cc	and,Given
R9318	T14542	T14535	conj	from,Given
R9319	T14427	T14424	nummod	1,sterolin
R9320	T14543	T14544	det	the,mouse
R9321	T14544	T14542	pobj	mouse,from
R9322	T14545	T14546	npadvmod	Abcg5,deficient
R9323	T14428	T14419	prep	in,location
R9324	T14546	T14544	amod	deficient,mouse
R9325	T14547	T14546	punct	-,deficient
R9326	T14548	T14536	nsubj	this,appear
R9327	T14429	T14430	det	the,mice
R9328	T14549	T14536	aux	does,appear
R9329	T14550	T14536	neg	not,appear
R9330	T14551	T14552	aux	to,be
R9331	T14430	T14428	pobj	mice,in
R9332	T14552	T14536	xcomp	be,appear
R9333	T14553	T14554	det	the,case
R9334	T14554	T14552	attr	case,be
R9335	T14431	T14430	nmod	Abcg8,mice
R9336	T14555	T14536	punct	.,appear
R9337	T14432	T14431	punct	-,Abcg8
R9338	T14557	T14558	advmod	Perhaps,made
R9339	T14559	T14558	prep	in,made
R9340	T14433	T14431	punct	/,Abcg8
R9341	T14560	T14561	det	the,model
R9342	T14561	T14559	pobj	model,in
R9343	T14434	T14431	punct	-,Abcg8
R9344	T14562	T14563	advmod	previously,published
R9345	T14563	T14561	amod	published,model
R9346	T14564	T14565	det	a,state
R9347	T14435	T14413	punct	.,are
R9348	T14565	T14558	nsubjpass	state,made
R9349	T14566	T14565	amod	non-physiologic,state
R9350	T14567	T14558	aux	has,made
R9351	T14437	T14438	prep	In,described
R9352	T14568	T14558	auxpass	been,made
R9353	T14569	T14570	dep	that,generates
R9354	T14570	T14558	ccomp	generates,made
R9355	T14571	T14572	det	these,data
R9356	T14439	T14440	det	this,context
R9357	T14572	T14570	dobj	data,generates
R9358	T14573	T14558	punct	.,made
R9359	T14575	T14576	advcl	Taken,suggest
R9360	T14440	T14437	pobj	context,In
R9361	T14577	T14575	advmod	together,Taken
R9362	T14578	T14576	punct	", ",suggest
R9363	T14579	T14580	det	these,models
R9364	T14580	T14576	nsubj	models,suggest
R9365	T14581	T14580	compound	animal,models
R9366	T14441	T14438	punct	", ",described
R9367	T14582	T14583	mark	that,have
R9368	T14583	T14576	advcl	have,suggest
R9369	T14584	T14585	compound	Abcg5,sterolin
R9370	T14442	T14438	nsubj	Plösch,described
R9371	T14585	T14583	nsubj	sterolin,have
R9372	T14586	T14585	punct	/,sterolin
R9373	T14587	T14585	punct	-,sterolin
R9374	T14443	T14444	advmod	et,al.
R9375	T14588	T14585	nummod	1,sterolin
R9376	T14589	T14585	cc	and,sterolin
R9377	T14590	T14591	compound	Abcg8,sterolin
R9378	T14444	T14442	advmod	al.,Plösch
R9379	T14591	T14585	conj	sterolin,sterolin
R9380	T14445	T14438	aux	have,described
R9381	T14446	T14447	det	an,mouse
R9382	T14592	T14591	punct	/,sterolin
R9383	T14447	T14438	dobj	mouse,described
R9384	T14593	T14591	punct	-,sterolin
R9385	T14594	T14591	nummod	2,sterolin
R9386	T14448	T14449	compound	Abcg5,sterolin
R9387	T14595	T14596	amod	independent,function
R9388	T14596	T14583	dobj	function,have
R9389	T14597	T14598	advmod	in,vivo
R9390	T14449	T14451	npadvmod	sterolin,deficient
R9391	T14598	T14583	advmod	vivo,have
R9392	T14599	T14583	cc	or,have
R9393	T14600	T14601	mark	that,are
R9394	T14450	T14449	punct	/,sterolin
R9395	T14601	T14583	conj	are,have
R9396	T14602	T14601	expl	there,are
R9397	T14603	T14601	attr	proteins,are
R9398	T14451	T14447	amod	deficient,mouse
R9399	T14452	T14449	punct	-,sterolin
R9401	T14453	T14449	nummod	1,sterolin
R9404	T14454	T14455	dep	that,maintains
R9406	T14455	T14447	relcl	maintains,mouse
R9407	T14456	T14457	det	the,ability
R9408	T14457	T14455	dobj	ability,maintains
R9411	T14458	T14459	aux	to,secrete
R9416	T14459	T14457	acl	secrete,ability
R9417	T14460	T14461	amod	biliary,cholesterol
R9418	T14461	T14459	dobj	cholesterol,secrete
R9419	T14462	T14459	prep	to,secrete
R9420	T14463	T14464	det	the,extent
R9421	T14464	T14462	pobj	extent,to
R9422	T14465	T14464	amod	same,extent
R9423	T14466	T14464	prep	as,extent
R9424	T14467	T14468	det	the,mice
R9425	T14468	T14466	pobj	mice,as
R9426	T14469	T14470	amod	wild,type
R9427	T14470	T14468	compound	type,mice
R9428	T14471	T14470	punct	-,type
R9429	T14472	T14455	cc	and,maintains
R9430	T14473	T14455	punct	", ",maintains
R9431	T14474	T14475	advmod	when,fed
R9432	T14475	T14476	advcl	fed,had
R9433	T14476	T14455	conj	had,maintains
R9434	T14477	T14478	det	a,agonist
R9435	T14478	T14475	dobj	agonist,fed
R9436	T14479	T14478	compound	LXR,agonist
R9437	T14480	T14476	punct	", ",had
R9438	T14481	T14482	amod	higher,expression
R9439	T14482	T14476	dobj	expression,had
R9440	T14483	T14484	compound	Abcg8,mRNA
R9441	T14484	T14482	compound	mRNA,expression
R9442	T14485	T14476	cc	and,had
R9443	T14486	T14476	conj	tended,had
R9444	T14487	T14488	aux	to,secrete
R9445	T14488	T14486	xcomp	secrete,tended
R9446	T14489	T14490	amod	more,cholesterol
R9447	T14490	T14488	dobj	cholesterol,secrete
R9448	T14491	T14490	amod	biliary,cholesterol
R9449	T14492	T14490	prep	than,cholesterol
R9450	T14493	T14494	amod	wild,type
R9451	T14494	T14496	compound	type,mice
R9452	T14495	T14494	punct	-,type
R9453	T14496	T14492	pobj	mice,than
R9454	T14497	T14498	punct	[,46
R9455	T14498	T14438	parataxis	46,described
R9456	T14499	T14498	punct	],46
R9457	T14500	T14438	punct	.,described
R9458	T14680	T14681	det	The,findings
R9459	T14681	T14683	nsubj	findings,are
R9460	T14682	T14681	amod	major,findings
R9461	T14684	T14681	prep	in,findings
R9462	T14685	T14686	det	this,study
R9463	T14686	T14684	pobj	study,in
R9464	T14687	T14683	punct	: ,are
R9465	T14688	T14689	punct	(,1
R9466	T14689	T14690	meta	1,is
R9467	T14690	T14683	ccomp	is,are
R9468	T14691	T14689	punct	),1
R9469	T14692	T14690	nsubj	disruption,is
R9470	T14693	T14692	prep	of,disruption
R9471	T14694	T14695	advmod	only,one
R9472	T14695	T14693	pobj	one,of
R9473	T14696	T14695	prep	of,one
R9474	T14697	T14698	det	the,genes
R9475	T14698	T14696	pobj	genes,of
R9476	T14699	T14698	nummod	two,genes
R9477	T14700	T14698	acl	comprising,genes
R9478	T14701	T14702	det	the,locus
R9479	T14702	T14700	dobj	locus,comprising
R9480	T14703	T14702	compound	STSL,locus
R9481	T14704	T14690	acomp	necessary,is
R9482	T14705	T14704	cc	and,necessary
R9483	T14706	T14704	conj	sufficient,necessary
R9484	T14707	T14708	aux	to,cause
R9485	T14708	T14706	xcomp	cause,sufficient
R9486	T14709	T14708	dobj	sitosterolemia,cause
R9487	T14710	T14690	punct	", ",is
R9488	T14711	T14712	punct	(,2
R9489	T14712	T14713	meta	2,leads
R9490	T14713	T14690	conj	leads,is
R9491	T14714	T14712	punct	),2
R9492	T14715	T14713	mark	that,leads
R9493	T14716	T14717	det	the,loss
R9494	T14717	T14713	nsubj	loss,leads
R9495	T14718	T14717	prep	of,loss
R9496	T14719	T14720	compound	Abcg8,sterolin
R9497	T14720	T14718	pobj	sterolin,of
R9498	T14721	T14720	punct	/,sterolin
R9499	T14722	T14720	punct	-,sterolin
R9500	T14723	T14720	nummod	2,sterolin
R9501	T14724	T14713	prep	to,leads
R9502	T14725	T14726	det	the,loss
R9503	T14726	T14724	pobj	loss,to
R9504	T14727	T14726	prep	of,loss
R9505	T14728	T14729	amod	biliary,secretion
R9506	T14729	T14727	pobj	secretion,of
R9507	T14730	T14729	compound	cholesterol,secretion
R9508	T14731	T14713	cc	and,leads
R9509	T14732	T14733	punct	(,3
R9510	T14733	T14734	meta	3,appears
R9511	T14734	T14713	conj	appears,leads
R9512	T14735	T14733	punct	),3
R9513	T14736	T14737	compound	Abcg5,sterolin
R9514	T14737	T14734	nsubj	sterolin,appears
R9515	T14738	T14737	punct	/,sterolin
R9516	T14739	T14737	punct	-,sterolin
R9517	T14740	T14737	nummod	1,sterolin
R9518	T14741	T14742	aux	to,expressed
R9519	T14742	T14734	xcomp	expressed,appears
R9520	T14743	T14742	auxpass	be,expressed
R9521	T14744	T14742	advmod	still,expressed
R9522	T14745	T14742	advmod	apically,expressed
R9523	T14746	T14742	punct	-,expressed
R9524	T14747	T14742	prep	despite,expressed
R9525	T14748	T14749	det	the,absence
R9526	T14749	T14747	pobj	absence,despite
R9527	T14750	T14749	prep	of,absence
R9528	T14751	T14752	compound	Abcg8,sterolin
R9529	T14752	T14750	pobj	sterolin,of
R9530	T14753	T14752	punct	/,sterolin
R9531	T14754	T14752	punct	-,sterolin
R9532	T14755	T14752	nummod	2,sterolin
R9533	T14756	T14683	punct	.,are
R9534	T14758	T14759	det	These,data
R9535	T14759	T14760	nsubj	data,support
R9536	T14761	T14760	advmod	strongly,support
R9537	T14762	T14763	det	the,role
R9538	T14763	T14760	dobj	role,support
R9539	T14764	T14763	amod	direct,role
R9540	T14765	T14763	prep	of,role
R9541	T14766	T14767	compound	Abcg8,sterolin
R9542	T14767	T14765	pobj	sterolin,of
R9543	T14768	T14767	punct	/,sterolin
R9544	T14769	T14767	punct	-,sterolin
R9545	T14770	T14767	nummod	2,sterolin
R9546	T14771	T14763	prep	as,role
R9547	T14772	T14773	det	a,player
R9548	T14773	T14771	pobj	player,as
R9549	T14774	T14773	amod	key,player
R9550	T14775	T14773	prep	in,player
R9551	T14776	T14777	amod	biliary,cholesterol
R9552	T14777	T14778	compound	cholesterol,secretion
R9553	T14778	T14775	pobj	secretion,in
R9554	T14779	T14760	punct	", ",support
R9555	T14780	T14781	mark	although,preserved
R9556	T14781	T14760	advcl	preserved,support
R9557	T14782	T14783	amod	biliary,sitosterol
R9558	T14783	T14784	compound	sitosterol,secretion
R9559	T14784	T14781	nsubjpass	secretion,preserved
R9560	T14785	T14781	auxpass	was,preserved
R9561	T14786	T14781	advmod	apparently,preserved
R9562	T14787	T14760	punct	.,support
R9563	T14789	T14790	det	These,data
R9564	T14790	T14791	nsubj	data,suggest
R9565	T14792	T14793	mark	that,are
R9566	T14793	T14791	ccomp	are,suggest
R9567	T14794	T14793	expl	there,are
R9568	T14795	T14793	attr	mechanisms,are
R9569	T14796	T14795	amod	other,mechanisms
R9570	T14797	T14796	prep	than,other
R9571	T14798	T14799	compound	Abcg8,sterolin
R9572	T14799	T14797	pobj	sterolin,than
R9573	T14800	T14799	punct	/,sterolin
R9574	T14801	T14799	punct	-,sterolin
R9575	T14802	T14799	nummod	2,sterolin
R9576	T14803	T14804	dep	that,allow
R9577	T14804	T14795	relcl	allow,mechanisms
R9578	T14805	T14804	prep	for,allow
R9579	T14806	T14807	det	the,secretion
R9580	T14807	T14805	pobj	secretion,for
R9581	T14808	T14807	prep	of,secretion
R9582	T14809	T14808	pobj	sitosterol,of
R9583	T14810	T14807	prep	into,secretion
R9584	T14811	T14810	pobj	bile,into
R9585	T14812	T14791	punct	.,suggest
R9589	T14817	T14814	prep	for,history
R9590	T14818	T14819	det	this,paper
R9591	T14819	T14817	pobj	paper,for
R9592	T14820	T14816	aux	can,accessed
R9593	T14821	T14816	auxpass	be,accessed
R9594	T14822	T14816	advmod	here,accessed
R9595	T14823	T14816	punct	:,accessed
R1	T258	T261	dep	model,results
R3	T262	T258	prep	of,model
R4	T263	T262	pobj	sitosterolemia,of
R5	T264	T261	punct	: ,results
R6	T265	T261	nsubj	absence,results
R7	T266	T265	prep	of,absence
R8	T267	T268	compound	Abcg8,sterolin
R9	T268	T266	pobj	sterolin,of
R11	T270	T268	punct	-,sterolin
R12	T271	T268	nummod	2,sterolin
R13	T272	T261	prep	in,results
R14	T273	T272	pobj	failure,in
R15	T274	T275	aux	to,secrete
R16	T275	T273	acl	secrete,failure
R17	T276	T277	amod	biliary,cholesterol
R18	T277	T275	dobj	cholesterol,secrete
R19	T282	T283	nsubj	Mutations,result
R20	T284	T282	prep	in,Mutations
R21	T285	T284	pobj	either,in
R22	T286	T285	prep	of,either
R23	T287	T288	nummod	two,genes
R24	T288	T286	pobj	genes,of
R25	T289	T288	acl	comprising,genes
R26	T290	T291	det	the,locus
R27	T291	T289	dobj	locus,comprising
R29	T293	T285	punct	", ",either
R30	T294	T295	npadvmod	ATP,binding
R31	T295	T297	amod	binding,cassette
R33	T297	T298	nmod	cassette,transporters
R34	T298	T303	compound	transporters,ABCG5
R39	T303	T285	appos	ABCG5,either
R40	T304	T303	punct	(,ABCG5
R41	T305	T303	acl	encoding,ABCG5
R42	T306	T305	dobj	sterolin,encoding
R43	T307	T306	punct	-,sterolin
R44	T308	T306	nummod	1,sterolin
R45	T309	T303	punct	),ABCG5
R46	T310	T303	cc	and,ABCG5
R47	T311	T303	conj	ABCG8,ABCG5
R48	T312	T311	punct	(,ABCG8
R49	T313	T311	acl	encoding,ABCG8
R50	T314	T313	dobj	sterolin,encoding
R51	T315	T314	punct	-,sterolin
R52	T316	T314	nummod	2,sterolin
R53	T317	T311	punct	),ABCG8
R54	T318	T283	punct	", ",result
R55	T319	T283	prep	in,result
R56	T320	T319	pobj	sitosterolemia,in
R57	T321	T320	punct	", ",sitosterolemia
R58	T322	T323	det	a,disorder
R59	T323	T320	appos	disorder,sitosterolemia
R63	T327	T323	prep	of,disorder
R64	T328	T329	compound	sterol,trafficking
R65	T329	T327	pobj	trafficking,of
R66	T330	T323	acl	characterized,disorder
R67	T331	T330	agent	by,characterized
R68	T332	T333	amod	increased,levels
R69	T333	T331	pobj	levels,by
R73	T337	T283	punct	.,result
R74	T339	T340	prep	Based,hypothesized
R75	T341	T339	prep	upon,Based
R76	T342	T343	det	the,genetics
R77	T343	T341	pobj	genetics,upon
R78	T344	T343	prep	of,genetics
R79	T345	T344	pobj	sitosterolemia,of
R80	T346	T340	punct	", ",hypothesized
R81	T347	T348	compound	ABCG5,sterolin
R82	T348	T340	nsubjpass	sterolin,hypothesized
R84	T350	T348	punct	-,sterolin
R85	T351	T348	nummod	1,sterolin
R86	T352	T348	cc	and,sterolin
R87	T353	T354	compound	ABCG8,sterolin
R88	T354	T348	conj	sterolin,sterolin
R90	T356	T354	punct	-,sterolin
R91	T357	T354	nummod	2,sterolin
R92	T358	T340	auxpass	are,hypothesized
R93	T359	T360	aux	to,function
R94	T360	T340	xcomp	function,hypothesized
R95	T361	T360	prep	as,function
R96	T362	T363	amod	obligate,heterodimers
R97	T363	T361	pobj	heterodimers,as
R98	T364	T340	punct	.,hypothesized
R99	T366	T367	det	No,difference
R100	T367	T369	nsubjpass	difference,described
R102	T370	T369	aux	has,described
R103	T371	T369	advmod	yet,described
R104	T372	T369	auxpass	been,described
R105	T373	T369	prep	in,described
R106	T374	T373	pobj	humans,in
R107	T375	T374	prep	with,humans
R108	T376	T377	amod	complete,defects
R109	T377	T375	pobj	defects,with
R110	T378	T377	prep	in,defects
R111	T379	T380	preconj	either,ABCG5
R112	T380	T378	pobj	ABCG5,in
R113	T381	T380	cc	or,ABCG5
R114	T382	T380	conj	ABCG8,ABCG5
R115	T383	T369	punct	.,described
R116	T385	T386	det	These,proteins
R117	T386	T387	nsubj	proteins,are
R118	T388	T387	punct	", ",are
R119	T389	T387	prep	based,are
R120	T390	T389	prep	upon,based
R121	T391	T392	det	the,defects
R122	T392	T390	pobj	defects,upon
R123	T393	T392	prep	in,defects
R124	T394	T393	pobj	humans,in
R125	T395	T387	punct	", ",are
R126	T396	T387	acomp	responsible,are
R127	T397	T396	prep	for,responsible
R128	T398	T397	pcomp	regulating,for
R129	T399	T400	amod	dietary,entry
R130	T400	T398	dobj	entry,regulating
R132	T402	T400	cc	and,entry
R133	T403	T404	amod	biliary,secretion
R134	T404	T400	conj	secretion,entry
R136	T406	T387	punct	.,are
R137	T410	T411	prep	In,created
R138	T412	T410	pobj	order,In
R139	T413	T414	aux	to,mimic
R140	T414	T412	acl	mimic,order
R141	T415	T416	det	the,disease
R142	T416	T414	dobj	disease,mimic
R144	T418	T411	punct	", ",created
R145	T419	T411	nsubj	we,created
R146	T420	T411	punct	", ",created
R147	T421	T411	prep	by,created
R148	T422	T423	det	a,disruption
R149	T423	T421	pobj	disruption,by
R151	T425	T411	punct	", ",created
R152	T426	T427	det	a,model
R153	T427	T411	dobj	model,created
R155	T429	T427	prep	of,model
R156	T430	T429	pobj	sitosterolemia,of
R157	T431	T427	acl	resulting,model
R158	T432	T431	prep	in,resulting
R159	T433	T434	nmod	Abcg8,sterolin
R160	T434	T436	nmod	sterolin,deficiency
R162	T436	T432	pobj	deficiency,in
R165	T439	T436	advmod	alone,deficiency
R166	T440	T411	punct	.,created
R167	T442	T443	amod	Homozygous,mice
R168	T443	T445	nsubj	mice,are
R170	T446	T445	acomp	viable,are
R171	T447	T445	cc	and,are
R172	T448	T445	conj	exhibit,are
R173	T449	T448	dobj	sitosterolemia,exhibit
R174	T450	T445	punct	.,are
R175	T454	T455	nsubj	Mice,have
R176	T456	T454	amod	deficient,Mice
R177	T457	T454	prep	in,Mice
R178	T458	T457	pobj	Abcg8,in
R179	T459	T460	advmod	significantly,increased
R180	T460	T461	amod	increased,plasma
R181	T461	T455	dobj	plasma,have
R182	T462	T461	cc	and,plasma
R183	T463	T464	compound	tissue,sterol
R184	T464	T466	compound	sterol,levels
R186	T466	T461	conj	levels,plasma
R187	T467	T461	punct	(,plasma
R188	T468	T461	appos	sitosterol,plasma
R189	T469	T468	cc	and,sitosterol
R190	T470	T468	conj	campesterol,sitosterol
R191	T471	T461	punct	),plasma
R192	T472	T461	amod	consistent,plasma
R193	T473	T472	prep	with,consistent
R194	T474	T473	pobj	sitosterolemia,with
R195	T475	T455	punct	.,have
R196	T477	T478	advmod	Interestingly,expressed
R197	T479	T478	punct	", ",expressed
R198	T480	T481	compound	Abcg5,sterolin
R199	T481	T478	nsubjpass	sterolin,expressed
R201	T483	T481	punct	-,sterolin
R202	T484	T481	nummod	1,sterolin
R203	T485	T478	auxpass	was,expressed
R204	T486	T478	prep	in,expressed
R205	T487	T488	preconj	both,liver
R206	T488	T486	pobj	liver,in
R207	T489	T488	cc	and,liver
R208	T490	T488	conj	intestine,liver
R209	T491	T478	prep	in,expressed
R210	T492	T493	compound	Abcg8,sterolin
R211	T493	T495	npadvmod	sterolin,deficient
R213	T495	T498	amod	deficient,mice
R216	T498	T491	pobj	mice,in
R217	T499	T478	cc	and,expressed
R218	T500	T478	conj	continued,expressed
R219	T501	T502	aux	to,show
R220	T502	T500	xcomp	show,continued
R221	T503	T504	det	an,expression
R222	T504	T502	dobj	expression,show
R224	T506	T478	punct	.,expressed
R225	T508	T509	advmod	Remarkably,had
R226	T510	T509	punct	", ",had
R227	T511	T512	npadvmod	Abcg8,deficient
R228	T512	T513	amod	deficient,mice
R229	T513	T509	nsubj	mice,had
R230	T514	T515	det	an,ability
R231	T515	T509	dobj	ability,had
R233	T517	T518	aux	to,secrete
R234	T518	T515	acl	secrete,ability
R235	T519	T518	dobj	cholesterol,secrete
R236	T520	T518	prep	into,secrete
R237	T521	T520	pobj	bile,into
R238	T522	T509	punct	", ",had
R239	T523	T509	cc	but,had
R240	T524	T525	advmod	still,maintained
R241	T525	T509	conj	maintained,had
R242	T526	T527	det	the,ability
R243	T527	T525	dobj	ability,maintained
R244	T528	T529	aux	to,secrete
R245	T529	T527	acl	secrete,ability
R246	T530	T529	dobj	sitosterol,secrete
R247	T531	T509	punct	.,had
R248	T533	T534	nsubj	We,report
R249	T535	T534	advmod	also,report
R250	T536	T537	det	an,phenotype
R251	T537	T534	dobj	phenotype,report
R253	T539	T537	prep	in,phenotype
R254	T540	T541	det	the,mice
R255	T541	T539	pobj	mice,in
R261	T547	T548	dep	that,are
R262	T548	T541	relcl	are,mice
R263	T549	T548	neg	not,are
R264	T550	T548	acomp	sitosterolemic,are
R265	T551	T548	punct	", ",are
R266	T552	T548	cc	but,are
R267	T553	T548	conj	have,are
R268	T554	T555	det	a,level
R269	T555	T553	dobj	level,have
R271	T557	T555	prep	of,level
R272	T558	T559	amod	biliary,secretion
R273	T559	T557	pobj	secretion,of
R275	T561	T553	advcl	relative,have
R276	T562	T561	prep	to,relative
R277	T563	T564	amod	wild,type
R278	T564	T566	compound	type,mice
R280	T566	T562	pobj	mice,to
R281	T567	T534	punct	.,report
R282	T571	T572	det	These,data
R283	T572	T573	nsubj	data,indicate
R284	T574	T575	mark	that,is
R285	T575	T573	advcl	is,indicate
R287	T577	T575	nsubj	sterolin,is
R288	T578	T577	punct	/,sterolin
R291	T581	T575	acomp	necessary,is
R292	T582	T581	prep	for,necessary
R293	T583	T584	amod	biliary,secretion
R294	T584	T582	pobj	secretion,for
R296	T586	T575	cc	and,is
R297	T587	T588	mark	that,has
R298	T588	T575	conj	has,is
R302	T592	T590	pobj	sterolin,of
R303	T593	T592	punct	/,sterolin
R306	T596	T597	det	a,effect
R307	T597	T588	dobj	effect,has
R310	T600	T597	prep	upon,effect
R311	T601	T602	amod	biliary,secretion
R312	T602	T600	pobj	secretion,upon
R314	T604	T597	prep	than,effect
R315	T605	T604	pobj	sitosterol,than
R316	T606	T573	punct	.,indicate
R317	T608	T609	mark	Since,preserved
R318	T609	T614	advcl	preserved,suggests
R320	T611	T609	nsubjpass	secretion,preserved
R321	T612	T611	compound	sitosterol,secretion
R323	T615	T609	punct	", ",preserved
R324	T616	T617	mark	although,elevated
R325	T617	T609	advcl	elevated,preserved
R327	T619	T617	prep	in,elevated
R328	T620	T621	det	the,mice
R329	T621	T619	pobj	mice,in
R331	T623	T614	punct	", ",suggests
R332	T624	T625	det	this,observation
R333	T625	T614	nsubj	observation,suggests
R334	T626	T627	mark	that,be
R335	T627	T614	ccomp	be,suggests
R341	T633	T631	pobj	sterolin,by
R342	T634	T633	punct	/,sterolin
R346	T638	T627	acomp	responsible,be
R347	T639	T638	prep	for,responsible
R348	T640	T641	poss	its,secretion
R349	T641	T639	pobj	secretion,for
R350	T642	T641	prep	into,secretion
R351	T643	T642	pobj	bile,into
R352	T644	T614	punct	.,suggests
R353	T257	T258	det	A,model
R359	T1078	T1079	nsubj	Absorption,is
R360	T1080	T1078	prep	of,Absorption
R361	T1081	T1082	amod	dietary,cholesterol
R362	T1082	T1080	pobj	cholesterol,of
R363	T1083	T1078	prep	from,Absorption
R364	T1084	T1085	det	the,intestine
R365	T1085	T1083	pobj	intestine,from
R366	T1086	T1087	det	an,part
R367	T1087	T1079	attr	part,is
R368	T1088	T1087	amod	important,part
R369	T1089	T1087	prep	of,part
R370	T1090	T1091	compound	cholesterol,homeostasis
R371	T1091	T1089	pobj	homeostasis,of
R372	T1092	T1079	cc	and,is
R373	T1093	T1079	conj	represents,is
R374	T1094	T1095	det	the,step
R375	T1095	T1093	dobj	step,represents
R376	T1096	T1095	amod	initial,step
R377	T1097	T1098	dep	that,allows
R378	T1098	T1095	relcl	allows,step
R379	T1099	T1100	amod	dietary,cholesterol
R380	T1100	T1101	nsubj	cholesterol,exert
R381	T1101	T1098	ccomp	exert,allows
R382	T1102	T1101	aux	to,exert
R383	T1103	T1104	poss	its,effects
R384	T1104	T1101	dobj	effects,exert
R385	T1105	T1104	amod	metabolic,effects
R386	T1106	T1079	punct	.,is
R387	T1108	T1109	det	A,diet
R388	T1109	T1112	nsubj	diet,contains
R389	T1110	T1109	amod	typical,diet
R390	T1111	T1109	compound	western,diet
R391	T1113	T1114	advmod	relatively,equal
R392	T1114	T1115	amod	equal,amounts
R393	T1115	T1112	dobj	amounts,contains
R394	T1116	T1115	prep	of,amounts
R395	T1117	T1118	nmod	cholesterol,sterols
R396	T1118	T1116	pobj	sterols,of
R397	T1119	T1117	cc	and,cholesterol
R398	T1120	T1117	conj	non-cholesterol,cholesterol
R399	T1121	T1118	punct	", ",sterols
R400	T1122	T1123	advmod	mainly,sterols
R401	T1123	T1118	appos	sterols,sterols
R402	T1124	T1123	compound	plant,sterols
R403	T1125	T1115	punct	", ",amounts
R404	T1126	T1127	prep	of,absorbed
R405	T1127	T1115	relcl	absorbed,amounts
R406	T1128	T1126	pobj	which,of
R407	T1129	T1130	quantmod	about,55
R408	T1130	T1131	nummod	55,%
R409	T1131	T1127	nsubjpass	%,absorbed
R410	T1132	T1131	prep	of,%
R411	T1133	T1134	det	the,cholesterol
R412	T1134	T1132	pobj	cholesterol,of
R413	T1135	T1134	amod	dietary,cholesterol
R414	T1136	T1127	auxpass	is,absorbed
R415	T1137	T1127	cc	and,absorbed
R416	T1138	T1127	conj	retained,absorbed
R417	T1139	T1138	prep	compared,retained
R418	T1140	T1139	prep	to,compared
R419	T1141	T1142	punct	~,1
R420	T1142	T1143	nummod	1,%
R421	T1143	T1140	pobj	%,to
R422	T1144	T1143	prep	of,%
R423	T1145	T1146	det	the,sterols
R424	T1146	T1144	pobj	sterols,of
R425	T1147	T1146	amod	dietary,sterols
R426	T1148	T1146	amod	non-cholesterol,sterols
R427	T1149	T1150	punct	[,1
R428	T1150	T1112	parataxis	1,contains
R429	T1151	T1152	punct	-,3
R430	T1152	T1150	prep	3,1
R431	T1153	T1150	punct	],1
R432	T1154	T1112	punct	.,contains
R433	T1156	T1157	nsubj	Schoenheimer,recognized
R434	T1158	T1159	amod	more,75
R435	T1159	T1161	nummod	75,years
R436	T1160	T1159	quantmod	than,75
R437	T1161	T1162	npadvmod	years,ago
R438	T1162	T1157	advmod	ago,recognized
R439	T1163	T1164	mark	that,absorbed
R440	T1164	T1157	ccomp	absorbed,recognized
R441	T1165	T1166	advmod	only,cholesterol
R442	T1166	T1164	nsubjpass	cholesterol,absorbed
R443	T1167	T1166	punct	", ",cholesterol
R444	T1168	T1166	cc	not,cholesterol
R445	T1169	T1170	amod	non-cholesterol,sterols
R446	T1170	T1166	conj	sterols,cholesterol
R447	T1171	T1164	punct	", ",absorbed
R448	T1172	T1164	auxpass	is,absorbed
R449	T1173	T1164	prep	in,absorbed
R450	T1174	T1175	det	the,intestine
R451	T1175	T1173	pobj	intestine,in
R452	T1176	T1164	punct	", ",absorbed
R453	T1177	T1178	mark	although,elucidated
R454	T1178	T1164	advcl	elucidated,absorbed
R455	T1179	T1180	det	the,mechanisms
R456	T1180	T1178	nsubjpass	mechanisms,elucidated
R457	T1181	T1180	amod	exact,mechanisms
R458	T1182	T1180	amod	molecular,mechanisms
R459	T1183	T1184	prep	by,occurs
R460	T1184	T1180	relcl	occurs,mechanisms
R461	T1185	T1183	pobj	which,by
R462	T1186	T1187	amod	preferential,absorption
R463	T1187	T1184	nsubj	absorption,occurs
R464	T1188	T1187	compound	cholesterol,absorption
R465	T1189	T1178	aux	has,elucidated
R466	T1190	T1178	neg	not,elucidated
R467	T1191	T1178	auxpass	been,elucidated
R468	T1192	T1178	advmod	fully,elucidated
R469	T1193	T1194	punct	[,4
R470	T1194	T1178	parataxis	4,elucidated
R471	T1195	T1194	punct	],4
R472	T1196	T1157	punct	.,recognized
R473	T1198	T1199	advmod	Similarly,excrete
R474	T1200	T1199	punct	", ",excrete
R475	T1201	T1202	mark	although,secretes
R476	T1202	T1199	advcl	secretes,excrete
R477	T1203	T1204	det	the,liver
R478	T1204	T1202	nsubj	liver,secretes
R479	T1205	T1206	amod	free,cholesterol
R480	T1206	T1202	dobj	cholesterol,secretes
R481	T1207	T1202	prep	into,secretes
R482	T1208	T1207	pobj	bile,into
R483	T1209	T1199	punct	", ",excrete
R484	T1210	T1199	nsubj	it,excrete
R485	T1211	T1199	aux	can,excrete
R486	T1212	T1199	advmod	preferentially,excrete
R487	T1213	T1214	amod	non-cholesterol,sterols
R488	T1214	T1199	dobj	sterols,excrete
R489	T1215	T1199	prep	into,excrete
R490	T1216	T1215	pobj	bile,into
R491	T1217	T1199	cc	and,excrete
R492	T1218	T1219	det	the,mechanism
R493	T1219	T1220	nsubj	mechanism,has
R494	T1220	T1199	conj	has,excrete
R495	T1221	T1219	punct	(,mechanism
R496	T1222	T1219	nmod	s,mechanism
R497	T1223	T1219	punct	),mechanism
R498	T1224	T1219	prep	of,mechanism
R499	T1225	T1226	det	this,process
R500	T1226	T1224	pobj	process,of
R501	T1227	T1220	advmod	yet,has
R502	T1228	T1229	aux	to,elucidated
R503	T1229	T1220	xcomp	elucidated,has
R504	T1230	T1229	auxpass	be,elucidated
R505	T1231	T1232	advmod	as,well
R506	T1232	T1229	advmod	well,elucidated
R507	T1233	T1220	punct	.,has
R508	T1235	T1236	advmod	Only,recently
R509	T1236	T1237	advmod	recently,revealed
R510	T1238	T1237	punct	", ",revealed
R511	T1239	T1237	prep	through,revealed
R512	T1240	T1241	det	the,study
R513	T1241	T1239	pobj	study,through
R514	T1242	T1241	prep	of,study
R515	T1243	T1244	det	a,disease
R516	T1244	T1242	pobj	disease,of
R517	T1245	T1244	amod	rare,disease
R518	T1246	T1244	amod	human,disease
R519	T1247	T1237	punct	", ",revealed
R520	T1248	T1237	aux	have,revealed
R521	T1249	T1237	nsubjpass	clues,revealed
R522	T1250	T1249	prep	to,clues
R523	T1251	T1252	det	this,process
R524	T1252	T1250	pobj	process,to
R525	T1253	T1237	auxpass	been,revealed
R526	T1254	T1237	punct	.,revealed
R527	T1256	T1257	nsubj	Sitosterolemia,is
R528	T1258	T1256	punct	(,Sitosterolemia
R529	T1259	T1256	appos	MIM,Sitosterolemia
R530	T1260	T1259	nummod	210250,MIM
R531	T1261	T1259	punct	", ",MIM
R532	T1262	T1263	advmod	also,known
R533	T1263	T1259	acl	known,MIM
R534	T1264	T1263	prep	as,known
R535	T1265	T1264	pobj	phytosterolemia,as
R536	T1266	T1256	punct	),Sitosterolemia
R537	T1267	T1268	det	a,disorder
R539	T1269	T1268	amod	rare,disorder
R540	T1270	T1268	amod	autosomal,disorder
R541	T1271	T1268	punct	", ",disorder
R542	T1272	T1273	advmod	recessively,inherited
R543	T1273	T1268	amod	inherited,disorder
R544	T1274	T1268	acl	characterized,disorder
R545	T1275	T1274	agent	by,characterized
R546	T1276	T1277	amod	elevated,levels
R548	T1278	T1277	nmod	blood,levels
R549	T1279	T1278	cc	and,blood
R550	T1280	T1278	conj	tissue,blood
R551	T1281	T1282	compound	plant,sterol
R552	T1282	T1277	compound	sterol,levels
R553	T1283	T1284	punct	[,5
R554	T1284	T1257	parataxis	5,is
R555	T1285	T1284	punct	],5
R556	T1286	T1257	punct	.,is
R557	T1288	T1289	amod	Affected,individuals
R558	T1289	T1290	nsubj	individuals,develop
R559	T1291	T1290	aux	can,develop
R560	T1292	T1293	nmod	tendon,xanthomas
R561	T1293	T1290	dative	xanthomas,develop
R562	T1294	T1292	cc	and,tendon
R563	T1295	T1294	punct	/,and
R564	T1296	T1294	cc	or,and
R565	T1297	T1292	conj	tuberous,tendon
R566	T1298	T1293	punct	", ",xanthomas
R567	T1299	T1300	amod	hemolytic,episodes
R568	T1300	T1293	conj	episodes,xanthomas
R569	T1301	T1300	punct	", ",episodes
R570	T1302	T1300	conj	arthralgias,episodes
R571	T1303	T1302	punct	", ",arthralgias
R572	T1304	T1302	conj	arthritis,arthralgias
R573	T1305	T1304	punct	", ",arthritis
R574	T1306	T1304	cc	and,arthritis
R575	T1307	T1308	amod	premature,atherosclerosis
R576	T1308	T1304	conj	atherosclerosis,arthritis
R577	T1309	T1290	punct	[,develop
R578	T1310	T1311	nummod	6,7
R579	T1311	T1290	dobj	7,develop
R580	T1312	T1311	punct	",",7
R581	T1313	T1290	punct	],develop
R582	T1314	T1290	punct	.,develop
R583	T1316	T1317	amod	Sitosterolemic,patients
R584	T1317	T1318	nsubj	patients,have
R585	T1319	T1320	advmod	diagnostically,elevated
R586	T1320	T1321	amod	elevated,levels
R587	T1321	T1318	dobj	levels,have
R588	T1322	T1323	compound	plasma,sterol
R589	T1323	T1321	compound	sterol,levels
R590	T1324	T1323	compound	plant,sterol
R591	T1325	T1321	punct	(,levels
R592	T1326	T1327	prep	for,sitosterol
R593	T1327	T1321	appos	sitosterol,levels
R594	T1328	T1326	pobj	example,for
R595	T1329	T1327	punct	", ",sitosterol
R596	T1330	T1327	punct	", ",sitosterol
R597	T1331	T1327	conj	campesterol,sitosterol
R598	T1332	T1331	punct	", ",campesterol
R599	T1333	T1331	conj	stigmasterol,campesterol
R600	T1334	T1333	punct	", ",stigmasterol
R601	T1335	T1333	cc	and,stigmasterol
R602	T1336	T1333	conj	avenosterol,stigmasterol
R603	T1337	T1336	punct	", ",avenosterol
R604	T1338	T1336	conj	etc.,avenosterol
R605	T1339	T1321	punct	),levels
R606	T1340	T1321	cc	and,levels
R607	T1341	T1342	poss	their,metabolites
R608	T1342	T1321	conj	metabolites,levels
R609	T1343	T1344	advmod	5,saturated
R610	T1344	T1342	amod	saturated,metabolites
R611	T1345	T1344	punct	-,saturated
R612	T1346	T1342	compound	stanol,metabolites
R613	T1347	T1342	punct	", ",metabolites
R614	T1348	T1349	advmod	particularly,sitostanol
R615	T1349	T1342	appos	sitostanol,metabolites
R616	T1350	T1342	punct	", ",metabolites
R617	T1351	T1342	prep	with,metabolites
R618	T1352	T1353	amod	normal,levels
R620	T1354	T1352	cc	or,normal
R621	T1355	T1356	advmod	only,moderately
R622	T1356	T1357	advmod	moderately,increased
R623	T1357	T1352	conj	increased,normal
R624	T1358	T1353	compound	cholesterol,levels
R625	T1359	T1318	punct	.,have
R626	T1361	T1362	amod	Clinical,studies
R627	T1362	T1363	nsubj	studies,shown
R628	T1364	T1363	aux	have,shown
R629	T1365	T1366	mark	that,hyperabsorb
R630	T1366	T1363	ccomp	hyperabsorb,shown
R631	T1367	T1368	amod	affected,individuals
R632	T1368	T1366	nsubj	individuals,hyperabsorb
R633	T1369	T1370	det	all,sterols
R634	T1370	T1366	dobj	sterols,hyperabsorb
R635	T1371	T1370	amod	dietary,sterols
R636	T1372	T1373	punct	(,lost
R637	T1373	T1370	parataxis	lost,sterols
R638	T1374	T1373	advmod	thus,lost
R639	T1375	T1373	aux	have,lost
R640	T1376	T1377	det	the,ability
R641	T1377	T1373	dobj	ability,lost
R642	T1378	T1379	aux	to,restrict
R643	T1379	T1377	acl	restrict,ability
R644	T1380	T1381	det	the,species
R645	T1381	T1379	dobj	species,restrict
R646	T1382	T1381	compound	sterol,species
R647	T1383	T1381	acl	absorbed,species
R648	T1384	T1373	punct	),lost
R649	T1385	T1366	cc	and,hyperabsorb
R650	T1386	T1387	aux	have,lost
R651	T1387	T1366	conj	lost,hyperabsorb
R652	T1388	T1389	det	the,ability
R653	T1389	T1387	dobj	ability,lost
R654	T1390	T1391	aux	to,excrete
R655	T1391	T1389	acl	excrete,ability
R656	T1392	T1391	dobj	sterols,excrete
R657	T1393	T1391	prep	into,excrete
R658	T1394	T1393	pobj	bile,into
R659	T1395	T1396	punct	[,9
R660	T1396	T1363	parataxis	9,shown
R661	T1397	T1396	nummod	8,9
R662	T1398	T1396	punct	",",9
R663	T1399	T1396	punct	],9
R664	T1400	T1363	punct	.,shown
R665	T1402	T1403	nsubj	Infusion,leads
R666	T1404	T1402	prep	of,Infusion
R667	T1405	T1404	pobj	sitosterol,of
R668	T1406	T1405	cc	and,sitosterol
R669	T1407	T1405	conj	cholesterol,sitosterol
R670	T1408	T1402	prep	into,Infusion
R671	T1409	T1410	amod	normal,individuals
R672	T1410	T1408	pobj	individuals,into
R673	T1411	T1403	prep	to,leads
R674	T1412	T1413	det	a,excretion
R675	T1413	T1411	pobj	excretion,to
R676	T1414	T1413	amod	rapid,excretion
R677	T1415	T1414	cc	and,rapid
R678	T1416	T1414	conj	preferential,rapid
R679	T1417	T1413	prep	of,excretion
R680	T1418	T1417	pobj	sitosterol,of
R681	T1419	T1413	prep	into,excretion
R682	T1420	T1419	pobj	bile,into
R683	T1421	T1403	punct	", ",leads
R684	T1422	T1403	cc	but,leads
R685	T1423	T1424	prep	in,is
R687	T1425	T1426	amod	sitosterolemic,individuals
R688	T1426	T1423	pobj	individuals,in
R689	T1427	T1428	amod	biliary,sterol
R690	T1428	T1429	compound	sterol,secretion
R691	T1429	T1424	nsubj	secretion,is
R692	T1430	T1431	advmod	almost,absent
R693	T1431	T1424	acomp	absent,is
R694	T1432	T1433	punct	[,9
R695	T1433	T1424	parataxis	9,is
R696	T1434	T1433	nummod	7,9
R697	T1435	T1433	punct	",",9
R698	T1436	T1433	punct	],9
R699	T1437	T1424	punct	.,is
R700	T1439	T1440	amod	Previous,studies
R701	T1440	T1441	nsubj	studies,suggested
R702	T1442	T1440	prep	on,studies
R703	T1443	T1442	pobj	sitosterolemia,on
R704	T1444	T1441	aux	have,suggested
R705	T1445	T1446	det	a,defect
R706	T1446	T1441	dobj	defect,suggested
R707	T1447	T1446	acl	involving,defect
R708	T1448	T1449	det	a,transporter
R709	T1449	T1447	dobj	transporter,involving
R710	T1450	T1449	amod	putative,transporter
R711	T1451	T1449	nmod	sterol,transporter
R712	T1452	T1449	punct	',transporter
R713	T1453	T1449	punct	',transporter
R714	T1454	T1449	acl	expressed,transporter
R715	T1455	T1454	prep	in,expressed
R716	T1456	T1457	det	the,border
R717	T1457	T1455	pobj	border,in
R718	T1458	T1457	amod	intestinal,border
R719	T1459	T1457	compound	brush,border
R720	T1460	T1457	cc	and,border
R721	T1461	T1460	punct	/,and
R722	T1462	T1460	cc	or,and
R723	T1463	T1464	det	the,membrane
R724	T1464	T1457	conj	membrane,border
R725	T1465	T1464	amod	hepatic,membrane
R726	T1466	T1464	amod	canalicular,membrane
R727	T1467	T1468	punct	[,11
R728	T1468	T1441	parataxis	11,suggested
R729	T1469	T1468	nummod	10,11
R730	T1470	T1468	punct	",",11
R731	T1471	T1468	punct	],11
R732	T1472	T1441	punct	.,suggested
R733	T1474	T1475	amod	Genetic,analyses
R734	T1475	T1476	nsubj	analyses,allowed
R735	T1477	T1475	prep	of,analyses
R736	T1478	T1479	compound	sitosterolemia,pedigrees
R737	T1479	T1477	pobj	pedigrees,of
R738	T1480	T1481	det	the,mapping
R739	T1481	T1476	dobj	mapping,allowed
R740	T1482	T1481	prep	of,mapping
R741	T1483	T1484	det	the,locus
R742	T1484	T1482	pobj	locus,of
R743	T1485	T1484	compound	STSL,locus
R744	T1486	T1481	prep	to,mapping
R745	T1487	T1488	amod	human,2p21
R746	T1488	T1486	pobj	2p21,to
R747	T1489	T1488	compound	chromosome,2p21
R748	T1490	T1488	punct	", ",2p21
R749	T1491	T1488	prep	between,2p21
R750	T1492	T1491	pobj	D2S2294,between
R751	T1493	T1492	cc	and,D2S2294
R752	T1494	T1492	conj	D2S2298,D2S2294
R753	T1495	T1496	punct	[,13
R754	T1496	T1476	parataxis	13,allowed
R755	T1497	T1496	nummod	12,13
R756	T1498	T1496	punct	",",13
R757	T1499	T1496	punct	],13
R758	T1500	T1476	punct	.,allowed
R759	T1502	T1503	prep	By,identified
R760	T1504	T1502	pcomp	using,By
R761	T1505	T1506	amod	positional,cloning
R762	T1506	T1507	compound	cloning,procedures
R763	T1507	T1504	dobj	procedures,using
R764	T1508	T1502	cc	or,By
R765	T1509	T1502	conj	by,By
R766	T1510	T1509	pcomp	screening,by
R767	T1511	T1510	prep	for,screening
R768	T1512	T1511	pobj	genes,for
R769	T1513	T1512	acl	induced,genes
R770	T1514	T1513	agent	by,induced
R771	T1515	T1514	pobj	exposure,by
R772	T1516	T1515	prep	to,exposure
R773	T1517	T1518	compound	LXR,agonists
R774	T1518	T1516	pobj	agonists,to
R775	T1519	T1503	punct	", ",identified
R776	T1520	T1521	nummod	two,groups
R777	T1521	T1503	nsubj	groups,identified
R778	T1522	T1523	cc	not,one
R779	T1523	T1524	nummod	one,genes
R780	T1524	T1503	dobj	genes,identified
R781	T1525	T1523	cc	but,one
R782	T1526	T1523	conj	two,one
R783	T1527	T1524	punct	", ",genes
R784	T1528	T1524	appos	ABCG5,genes
R785	T1529	T1528	cc	and,ABCG5
R786	T1530	T1528	conj	ABCG8,ABCG5
R787	T1531	T1524	punct	", ",genes
R788	T1532	T1533	dep	which,encode
R789	T1533	T1524	relcl	encode,genes
R790	T1534	T1535	det	the,sterolin
R791	T1535	T1533	dobj	sterolin,encode
R792	T1536	T1535	compound	proteins,sterolin
R793	T1537	T1535	punct	-,sterolin
R794	T1538	T1535	nummod	1,sterolin
R795	T1539	T1535	cc	and,sterolin
R796	T1540	T1535	conj	sterolin,sterolin
R797	T1541	T1540	punct	-,sterolin
R798	T1542	T1540	nummod	2,sterolin
R799	T1543	T1535	punct	", ",sterolin
R800	T1544	T1545	dep	mutations,cause
R801	T1545	T1535	relcl	cause,sterolin
R802	T1546	T1544	prep	of,mutations
R803	T1547	T1546	pobj	which,of
R804	T1548	T1545	dobj	sitosterolemia,cause
R805	T1549	T1550	punct	[,14
R806	T1550	T1503	parataxis	14,identified
R807	T1551	T1552	punct	-,16
R808	T1552	T1550	prep	16,14
R809	T1553	T1550	punct	],14
R810	T1554	T1503	punct	.,identified
R811	T1556	T1557	advmod	Interestingly,is
R812	T1558	T1557	punct	", ",is
R813	T1559	T1560	amod	complete,mutation
R814	T1560	T1557	nsubj	mutation,is
R815	T1561	T1560	prep	in,mutation
R816	T1562	T1563	preconj	either,ABCG5
R817	T1563	T1561	pobj	ABCG5,in
R818	T1564	T1563	advmod	alone,ABCG5
R819	T1565	T1563	cc	or,ABCG5
R820	T1566	T1563	conj	ABCG8,ABCG5
R821	T1567	T1566	advmod	alone,ABCG8
R822	T1568	T1569	preconj	not,necessary
R823	T1569	T1557	acomp	necessary,is
R824	T1570	T1568	advmod	only,not
R825	T1571	T1569	punct	", ",necessary
R826	T1572	T1569	cc	but,necessary
R827	T1573	T1569	conj	sufficient,necessary
R828	T1574	T1575	aux	to,cause
R829	T1575	T1573	xcomp	cause,sufficient
R830	T1576	T1577	det	the,disease
R831	T1577	T1575	dobj	disease,cause
R832	T1578	T1579	punct	[,14
R833	T1579	T1557	parataxis	14,is
R834	T1580	T1579	punct	],14
R835	T1581	T1557	punct	.,is
R836	T1583	T1584	prep	To,identified
R837	T1585	T1583	pobj	date,To
R838	T1586	T1584	punct	", ",identified
R839	T1587	T1588	det	no,patient
R840	T1588	T1584	nsubjpass	patient,identified
R841	T1589	T1588	prep	with,patient
R842	T1590	T1589	pobj	sitosterolemia,with
R843	T1591	T1584	aux	has,identified
R844	T1592	T1584	auxpass	been,identified
R845	T1593	T1584	prep	with,identified
R846	T1594	T1593	pobj	mutations,with
R847	T1595	T1594	prep	in,mutations
R848	T1596	T1597	det	both,genes
R849	T1597	T1595	pobj	genes,in
R850	T1598	T1584	punct	.,identified
R851	T1600	T1601	prep	Based,hypothesized
R852	T1602	T1600	prep	upon,Based
R853	T1603	T1604	det	these,data
R854	T1604	T1602	pobj	data,upon
R855	T1605	T1604	punct	", ",data
R856	T1606	T1604	cc	and,data
R857	T1607	T1608	det	the,similarity
R858	T1608	T1604	conj	similarity,data
R859	T1609	T1608	prep	of,similarity
R860	T1610	T1611	det	the,phenotype
R861	T1611	T1609	pobj	phenotype,of
R862	T1612	T1611	amod	clinical,phenotype
R863	T1613	T1612	cc	and,clinical
R864	T1614	T1612	conj	biochemical,clinical
R865	T1615	T1601	punct	", ",hypothesized
R866	T1616	T1601	nsubjpass	sterolin,hypothesized
R867	T1617	T1616	punct	-,sterolin
R868	T1618	T1616	nummod	1,sterolin
R869	T1619	T1616	cc	and,sterolin
R870	T1620	T1616	conj	sterolin,sterolin
R871	T1621	T1620	punct	-,sterolin
R872	T1622	T1620	nummod	2,sterolin
R873	T1623	T1601	auxpass	are,hypothesized
R874	T1624	T1625	aux	to,function
R875	T1625	T1601	xcomp	function,hypothesized
R876	T1626	T1625	prep	as,function
R877	T1627	T1628	amod	obligate,heterodimers
R878	T1628	T1626	pobj	heterodimers,as
R879	T1629	T1601	punct	.,hypothesized
R880	T1631	T1632	nsubjpass	This,supported
R881	T1633	T1632	auxpass	is,supported
R882	T1634	T1632	advmod	also,supported
R883	T1635	T1632	agent	by,supported
R884	T1636	T1637	det	the,fact
R885	T1637	T1635	pobj	fact,by
R886	T1638	T1639	mark	that,is
R887	T1639	T1637	acl	is,fact
R888	T1640	T1639	nsubj	each,is
R889	T1641	T1640	prep	of,each
R890	T1642	T1643	det	these,proteins
R891	T1643	T1641	pobj	proteins,of
R892	T1644	T1645	det	a,transporter
R893	T1645	T1639	attr	transporter,is
R894	T1646	T1645	punct	',transporter
R895	T1647	T1645	compound	half,transporter
R896	T1648	T1645	punct	-,transporter
R897	T1649	T1645	punct	',transporter
R898	T1650	T1645	punct	", ",transporter
R899	T1651	T1645	acl	containing,transporter
R900	T1652	T1653	nummod	six,domains
R901	T1653	T1651	dobj	domains,containing
R902	T1654	T1653	compound	transmembrane,domains
R903	T1655	T1653	punct	", ",domains
R904	T1656	T1653	cc	and,domains
R905	T1657	T1656	neg	not,and
R906	T1658	T1659	det	the,transmembrane
R907	T1659	T1653	conj	transmembrane,domains
R908	T1660	T1659	amod	classical,transmembrane
R909	T1661	T1659	nummod	12,transmembrane
R910	T1662	T1659	amod	characteristic,transmembrane
R911	T1663	T1662	prep	of,characteristic
R912	T1664	T1665	amod	functional,transporters
R913	T1665	T1663	pobj	transporters,of
R914	T1666	T1665	compound	ABC,transporters
R915	T1667	T1632	punct	.,supported
R916	T1669	T1670	det	The,function
R917	T1670	T1672	nsubj	function,remains
R918	T1671	T1670	amod	exact,function
R919	T1673	T1670	prep	of,function
R920	T1674	T1675	det	these,proteins
R921	T1675	T1673	pobj	proteins,of
R922	T1676	T1675	nummod	two,proteins
R923	T1677	T1672	advmod	still,remains
R924	T1678	T1672	acomp	unknown,remains
R925	T1679	T1672	cc	but,remains
R926	T1680	T1681	amod	recent,studies
R927	T1681	T1682	nsubj	studies,shown
R928	T1682	T1672	conj	shown,remains
R929	T1683	T1682	aux	have,shown
R930	T1684	T1685	mark	that,results
R931	T1685	T1682	ccomp	results,shown
R932	T1686	T1685	nsubj	overexpression,results
R933	T1687	T1686	prep	of,overexpression
R934	T1688	T1689	compound	ABCG5,ABCG8
R935	T1689	T1687	pobj	ABCG8,of
R936	T1690	T1689	punct	/,ABCG8
R937	T1691	T1686	prep	in,overexpression
R938	T1692	T1693	det	a,model
R939	T1693	T1691	pobj	model,in
R940	T1694	T1695	amod	transgenic,mouse
R941	T1695	T1693	compound	mouse,model
R942	T1696	T1685	prep	in,results
R943	T1697	T1698	det	an,concentration
R944	T1698	T1696	pobj	concentration,in
R945	T1699	T1698	amod	increased,concentration
R946	T1700	T1701	amod	biliary,cholesterol
R947	T1701	T1698	compound	cholesterol,concentration
R948	T1702	T1698	cc	and,concentration
R949	T1703	T1704	det	a,reduction
R950	T1704	T1698	conj	reduction,concentration
R951	T1705	T1706	nummod	50,%
R952	T1706	T1704	compound	%,reduction
R953	T1707	T1704	prep	in,reduction
R954	T1708	T1709	det	the,absorption
R955	T1709	T1707	pobj	absorption,in
R956	T1710	T1709	amod	fractional,absorption
R957	T1711	T1709	prep	of,absorption
R958	T1712	T1713	amod	dietary,cholesterol
R959	T1713	T1711	pobj	cholesterol,of
R960	T1714	T1715	punct	[,17
R961	T1715	T1682	parataxis	17,shown
R962	T1716	T1715	punct	],17
R963	T1717	T1682	punct	.,shown
R964	T1719	T1720	nsubjpass	It,suggested
R965	T1721	T1720	aux	has,suggested
R966	T1722	T1720	auxpass	been,suggested
R967	T1723	T1724	mark	that,act
R968	T1724	T1720	ccomp	act,suggested
R969	T1725	T1724	nsubj	ABCG5,act
R970	T1726	T1725	cc	and,ABCG5
R971	T1727	T1725	conj	ABCG8,ABCG5
R972	T1728	T1724	prep	as,act
R973	T1729	T1730	amod	mutual,chaperones
R974	T1730	T1728	pobj	chaperones,as
R975	T1731	T1730	prep	for,chaperones
R976	T1732	T1733	preconj	both,maturation
R977	T1733	T1731	pobj	maturation,for
R978	T1734	T1733	cc	and,maturation
R979	T1735	T1736	amod	correct,targeting
R980	T1736	T1733	conj	targeting,maturation
R981	T1737	T1736	amod	apical,targeting
R982	T1738	T1720	punct	.,suggested
R983	T1740	T1741	nsubjpass	Co-expression,required
R984	T1742	T1740	prep	of,Co-expression
R985	T1743	T1742	pobj	both,of
R986	T1744	T1741	auxpass	was,required
R987	T1745	T1741	prep	for,required
R988	T1746	T1747	amod	apical,trafficking
R989	T1747	T1745	pobj	trafficking,for
R990	T1748	T1747	prep	in,trafficking
R991	T1749	T1750	det	a,line
R992	T1750	T1748	pobj	line,in
R993	T1751	T1750	amod	polarized,line
R994	T1752	T1750	compound	cell,line
R995	T1753	T1748	punct	", ",in
R996	T1754	T1748	cc	as,in
R997	T1755	T1754	advmod	well,as
R998	T1756	T1757	advmod	in,vivo
R999	T1757	T1748	conj	vivo,in
R1000	T1758	T1741	advcl	using,required
R1001	T1759	T1760	nmod	Abcg5,Abcg8
R1002	T1760	T1762	nmod	Abcg8,knockout
R1003	T1761	T1760	punct	/,Abcg8
R1004	T1762	T1765	compound	knockout,mice
R1005	T1763	T1762	advmod	double,knockout
R1006	T1764	T1762	punct	-,knockout
R1007	T1765	T1758	dobj	mice,using
R1008	T1766	T1767	punct	[,19
R1009	T1767	T1741	parataxis	19,required
R1010	T1768	T1767	nummod	18,19
R1011	T1769	T1767	punct	",",19
R1012	T1770	T1767	punct	],19
R1013	T1771	T1741	punct	.,required
R1014	T1773	T1774	advmod	However,developed
R1015	T1775	T1774	punct	", ",developed
R1016	T1776	T1777	det	no,assay
R1017	T1837	T1838	preconj	both,Abcg5
R1018	T1777	T1774	nsubjpass	assay,developed
R1019	T1838	T1836	dobj	Abcg5,disrupting
R1020	T1778	T1777	amod	functional,assay
R1021	T1839	T1838	cc	and,Abcg5
R1022	T1840	T1838	conj	Abcg8,Abcg5
R1023	T1779	T1774	aux	has,developed
R1024	T1841	T1836	advmod	simultaneously,disrupting
R1025	T1842	T1835	prep	in,results
R1026	T1843	T1844	det	a,phenotype
R1027	T1780	T1774	advmod	yet,developed
R1028	T1844	T1842	pobj	phenotype,in
R1029	T1845	T1844	amod	similar,phenotype
R1030	T1846	T1845	prep	to,similar
R1031	T1781	T1774	auxpass	been,developed
R1032	T1847	T1846	pobj	that,to
R1033	T1848	T1847	prep	of,that
R1034	T1849	T1850	det	the,disease
R1035	T1850	T1848	pobj	disease,of
R1036	T1851	T1850	amod	human,disease
R1037	T1782	T1783	aux	to,demonstrate
R1038	T1852	T1853	punct	[,20
R1039	T1853	T1835	parataxis	20,results
R1040	T1854	T1853	punct	],20
R1041	T1783	T1774	advcl	demonstrate,developed
R1042	T1855	T1835	punct	.,results
R1043	T1857	T1858	advmod	However,is
R1044	T1784	T1785	mark	whether,function
R1045	T1859	T1858	punct	", ",is
R1046	T1785	T1783	advcl	function,demonstrate
R1047	T1860	T1858	nsubj	it,is
R1048	T1861	T1858	acomp	important,is
R1049	T1862	T1863	aux	to,point
R1050	T1786	T1785	cc	or,function
R1051	T1863	T1858	xcomp	point,is
R1052	T1864	T1863	prt	out,point
R1053	T1865	T1866	mark	that,caused
R1054	T1787	T1785	neg	not,function
R1055	T1866	T1863	ccomp	caused,point
R1056	T1867	T1868	det	the,disease
R1057	T1868	T1866	nsubjpass	disease,caused
R1058	T1788	T1789	det	the,transporters
R1059	T1789	T1785	nsubj	transporters,function
R1060	T1869	T1868	amod	human,disease
R1061	T1870	T1866	auxpass	is,caused
R1062	T1871	T1866	agent	by,caused
R1063	T1872	T1873	amod	complete,mutation
R1064	T1873	T1871	pobj	mutation,by
R1065	T1790	T1789	nummod	two,transporters
R1066	T1874	T1873	prep	in,mutation
R1067	T1875	T1874	pobj	one,in
R1068	T1876	T1875	cc	or,one
R1069	T1791	T1789	compound	half,transporters
R1070	T1877	T1878	det	the,other
R1071	T1878	T1879	nmod	other,gene
R1072	T1879	T1875	conj	gene,one
R1073	T1792	T1789	punct	-,transporters
R1074	T1880	T1875	punct	", ",one
R1075	T1881	T1875	cc	but,one
R1076	T1793	T1785	prep	as,function
R1077	T1882	T1881	neg	not,but
R1078	T1794	T1795	nmod	homo,heterodimers
R1079	T1883	T1875	conj	both,one
R1080	T1795	T1793	pobj	heterodimers,as
R1081	T1884	T1883	advmod	simultaneously,both
R1082	T1885	T1858	punct	.,is
R1083	T1796	T1795	punct	-,heterodimers
R1084	T1887	T1888	nsubj	We,hypothesized
R1085	T1889	T1890	mark	that,result
R1086	T1890	T1888	ccomp	result,hypothesized
R1087	T1797	T1795	cc	or,heterodimers
R1088	T1891	T1890	nsubj	disruption,result
R1089	T1892	T1891	prep	of,disruption
R1090	T1893	T1894	advmod	only,one
R1091	T1798	T1785	cc	and,function
R1092	T1894	T1892	pobj	one,of
R1093	T1895	T1894	prep	of,one
R1094	T1896	T1897	det	the,transporters
R1095	T1799	T1800	mark	whether,pump
R1096	T1897	T1895	pobj	transporters,of
R1097	T1898	T1897	compound	half,transporters
R1098	T1800	T1785	conj	pump,function
R1099	T1899	T1897	punct	-,transporters
R1100	T1900	T1890	aux	would,result
R1101	T1901	T1890	prep	in,result
R1102	T1801	T1800	nsubj	they,pump
R1103	T1902	T1901	pobj	sitosterolemia,in
R1104	T1903	T1890	cc	and,result
R1105	T1802	T1800	advmod	selectively,pump
R1106	T1904	T1905	amod	selective,loss
R1107	T1905	T1906	nsubj	loss,show
R1108	T1906	T1890	conj	show,result
R1109	T1803	T1800	dobj	sterols,pump
R1110	T1907	T1905	prep	of,loss
R1111	T1908	T1909	det	either,sterolin
R1112	T1909	T1907	pobj	sterolin,of
R1113	T1804	T1774	punct	.,developed
R1114	T1806	T1807	advmod	Likewise,has
R1115	T1910	T1906	aux	may,show
R1116	T1911	T1912	det	a,effect
R1117	T1912	T1906	dobj	effect,show
R1118	T1913	T1912	amod	differential,effect
R1119	T1914	T1912	prep	on,effect
R1120	T1808	T1807	punct	", ",has
R1121	T1915	T1916	det	the,secretion
R1122	T1916	T1914	pobj	secretion,on
R1123	T1917	T1916	amod	biliary,secretion
R1124	T1809	T1810	det	the,localization
R1125	T1918	T1916	prep	of,secretion
R1126	T1919	T1918	pobj	sterols,of
R1127	T1920	T1916	cc	or,secretion
R1128	T1810	T1807	nsubj	localization,has
R1129	T1921	T1922	amod	intestinal,absorption
R1130	T1922	T1916	conj	absorption,secretion
R1131	T1923	T1922	prep	of,absorption
R1132	T1811	T1810	amod	cellular,localization
R1133	T1924	T1923	pobj	sterols,of
R1134	T1925	T1888	punct	.,hypothesized
R1135	T1812	T1810	prep	of,localization
R1136	T1927	T1928	det	These,studies
R1137	T1928	T1929	nsubj	studies,are
R1138	T1930	T1929	acomp	difficult,are
R1139	T1931	T1932	aux	to,test
R1140	T1813	T1814	det	these,proteins
R1141	T1932	T1930	advcl	test,difficult
R1142	T1933	T1932	prep	in,test
R1143	T1934	T1933	pobj	humans,in
R1144	T1814	T1812	pobj	proteins,of
R1145	T1935	T1929	punct	", ",are
R1146	T1936	T1937	mark	as,are
R1147	T1937	T1929	advcl	are,are
R1148	T1815	T1814	nummod	two,proteins
R1149	T1938	T1939	det	the,organs
R1150	T1939	T1937	nsubj	organs,are
R1151	T1940	T1939	compound	target,organs
R1152	T1816	T1807	advmod	yet,has
R1153	T1941	T1942	advmod	relatively,inaccessible
R1154	T1942	T1937	acomp	inaccessible,are
R1155	T1817	T1818	aux	to,shown
R1156	T1818	T1807	xcomp	shown,has
R1157	T1819	T1818	auxpass	be,shown
R1158	T1943	T1929	punct	.,are
R1159	T1820	T1818	prep	in,shown
R1160	T1945	T1946	prep	In,report
R1161	T1821	T1822	det	a,model
R1162	T1947	T1945	pobj	order,In
R1163	T1948	T1949	aux	to,test
R1164	T1949	T1947	acl	test,order
R1165	T1822	T1820	pobj	model,in
R1166	T1950	T1951	det	this,hypothesis
R1167	T1951	T1949	dobj	hypothesis,test
R1168	T1823	T1822	amod	deficient,model
R1169	T1952	T1946	punct	", ",report
R1170	T1953	T1946	nsubj	we,report
R1171	T1824	T1823	prep	in,deficient
R1172	T1954	T1955	det	a,model
R1173	T1955	T1946	dobj	model,report
R1174	T1956	T1955	compound	mouse,model
R1175	T1825	T1826	preconj	either,Abcg5
R1176	T1957	T1958	dep	that,has
R1177	T1958	T1955	relcl	has,model
R1178	T1959	T1960	det	a,mutation
R1179	T1826	T1824	pobj	Abcg5,in
R1180	T1960	T1958	dobj	mutation,has
R1181	T1961	T1960	amod	selective,mutation
R1182	T1962	T1960	amod	genetic,mutation
R1183	T1827	T1826	cc	or,Abcg5
R1184	T1963	T1960	prep	of,mutation
R1185	T1964	T1963	pobj	Abcg8,of
R1186	T1965	T1946	punct	.,report
R1187	T1828	T1826	conj	Abcg8,Abcg5
R1188	T1967	T1968	nsubj	Mice,exhibit
R1189	T1969	T1967	amod	homozygous,Mice
R1190	T1970	T1969	prep	for,homozygous
R1191	T1971	T1972	compound	Abcg8,loss
R1192	T1829	T1826	advmod	alone,Abcg5
R1193	T1830	T1807	punct	.,has
R1194	T1972	T1970	pobj	loss,for
R1195	T1973	T1968	dobj	many,exhibit
R1196	T1974	T1973	prep	of,many
R1197	T1832	T1833	det	A,model
R1198	T1975	T1976	det	the,characteristics
R1199	T1976	T1974	pobj	characteristics,of
R1200	T1977	T1976	prep	of,characteristics
R1201	T1978	T1979	det	the,disease
R1202	T1833	T1835	nsubj	model,results
R1203	T1979	T1977	pobj	disease,of
R1204	T1980	T1979	amod	human,disease
R1205	T1834	T1833	compound	mouse,model
R1206	T1981	T1979	appos	sitosterolemia,disease
R1207	T1982	T1968	punct	.,exhibit
R1208	T1836	T1833	acl	disrupting,model
R1209	T1984	T1985	prep	In,report
R1210	T1986	T1984	pobj	addition,In
R1211	T1987	T1985	punct	", ",report
R1212	T1988	T1985	nsubj	we,report
R1213	T1989	T1985	dobj	studies,report
R1214	T1990	T1989	prep	of,studies
R1215	T1991	T1992	amod	biliary,secretion
R1216	T1992	T1990	pobj	secretion,of
R1217	T1993	T1992	compound	sterol,secretion
R1218	T1994	T1995	dep	that,show
R1219	T1995	T1989	relcl	show,studies
R1220	T1996	T1997	mark	that,secrete
R1221	T1997	T1995	ccomp	secrete,show
R1222	T1998	T1997	prep	despite,secrete
R1223	T1999	T2000	amod	forced,secretion
R1224	T2000	T1998	pobj	secretion,despite
R1225	T2001	T1997	punct	", ",secrete
R1226	T2002	T2003	npadvmod	Abcg8,deficient
R1227	T2003	T2005	amod	deficient,animals
R1228	T2004	T2003	punct	-,deficient
R1229	T2005	T1997	nsubj	animals,secrete
R1230	T2006	T1997	aux	can,secrete
R1231	T2007	T1997	neg	not,secrete
R1232	T2008	T1997	dobj	cholesterol,secrete
R1233	T2009	T1997	prep	into,secrete
R1234	T2010	T2009	pobj	bile,into
R1235	T2011	T1997	punct	", ",secrete
R1236	T2012	T2013	mark	although,seemed
R1237	T2013	T1997	advcl	seemed,secrete
R1238	T2014	T2015	compound	sitosterol,secretion
R1239	T2015	T2013	nsubj	secretion,seemed
R1240	T2016	T2013	oprd	unaffected,seemed
R1241	T2017	T1985	punct	.,report
R1242	T2220	T2221	compound	Targeting,vector
R1243	T2221	T2222	compound	vector,construction
R1244	T2223	T2222	cc	and,construction
R1245	T2224	T2222	conj	generation,construction
R1246	T2225	T2224	prep	of,generation
R1247	T2226	T2227	compound	knockout,mice
R1248	T2227	T2225	pobj	mice,of
R1249	T2229	T2230	det	A,library
R1250	T2230	T2239	nsubjpass	library,screened
R1251	T2231	T2232	npadvmod	mouse,genomic
R1252	T2232	T2230	amod	genomic,library
R1253	T2233	T2234	amod	bacterial,chromosome
R1254	T2234	T2230	nmod	chromosome,library
R1255	T2235	T2234	amod	artificial,chromosome
R1256	T2236	T2234	punct	(,chromosome
R1257	T2237	T2234	appos	BAC,chromosome
R1258	T2238	T2230	punct	),library
R1259	T2240	T2241	punct	(,Huntsville
R1260	T2241	T2230	parataxis	Huntsville,library
R1261	T2242	T2241	dep	CitbCJ7,Huntsville
R1262	T2243	T2241	punct	", ",Huntsville
R1263	T2244	T2245	compound	ES,cell
R1264	T2245	T2246	compound	cell,129Sv
R1265	T2246	T2241	dep	129Sv,Huntsville
R1266	T2247	T2246	compound	line,129Sv
R1267	T2248	T2246	punct	/,129Sv
R1268	T2249	T2241	punct	", ",Huntsville
R1269	T2250	T2251	compound	Research,Genetics
R1270	T2251	T2241	dep	Genetics,Huntsville
R1271	T2252	T2251	punct	", ",Genetics
R1272	T2253	T2251	amod	Inc.,Genetics
R1273	T2254	T2241	punct	", ",Huntsville
R1274	T2255	T2241	punct	", ",Huntsville
R1275	T2256	T2241	npadvmod	AL,Huntsville
R1276	T2257	T2241	punct	", ",Huntsville
R1277	T2258	T2241	npadvmod	USA,Huntsville
R1278	T2259	T2241	punct	),Huntsville
R1279	T2260	T2239	auxpass	was,screened
R1280	T2261	T2239	prep	by,screened
R1281	T2262	T2261	pcomp	using,by
R1282	T2263	T2262	dobj	primers,using
R1283	T2264	T2263	acl	designed,primers
R1284	T2265	T2264	prep	from,designed
R1285	T2266	T2267	det	the,sequences
R1286	T2267	T2265	pobj	sequences,from
R1287	T2268	T2267	prep	of,sequences
R1288	T2269	T2270	nmod	mouse,cDNA
R1289	T2270	T2268	pobj	cDNA,of
R1290	T2271	T2270	nmod	Abcg5,cDNA
R1291	T2272	T2271	cc	and,Abcg5
R1292	T2273	T2271	conj	Abcg8,Abcg5
R1293	T2274	T2275	mark	as,reported
R1294	T2275	T2262	advcl	reported,using
R1295	T2276	T2275	advmod	previously,reported
R1296	T2277	T2278	punct	[,21
R1297	T2278	T2239	parataxis	21,screened
R1298	T2279	T2278	punct	],21
R1299	T2280	T2239	punct	.,screened
R1300	T2282	T2283	det	A,clone
R1301	T2283	T2286	nsubjpass	clone,used
R1302	T2284	T2283	amod	positive,clone
R1303	T2285	T2283	compound	BAC,clone
R1304	T2287	T2286	auxpass	was,used
R1305	T2288	T2286	prep	as,used
R1306	T2289	T2290	det	a,template
R1307	T2290	T2288	pobj	template,as
R1308	T2291	T2292	aux	to,amplify
R1309	T2292	T2286	advcl	amplify,used
R1310	T2293	T2294	amod	genomic,fragments
R1311	T2294	T2292	dobj	fragments,amplify
R1312	T2295	T2294	compound	DNA,fragments
R1313	T2296	T2294	prep	of,fragments
R1314	T2297	T2296	pobj	Abcg8,of
R1315	T2298	T2286	punct	.,used
R1316	T2300	T2301	amod	Long,fragment
R1317	T2301	T2303	nsubjpass	fragment,performed
R1318	T2302	T2301	punct	-,fragment
R1319	T2304	T2305	compound	polymerase,reaction
R1320	T2305	T2301	appos	reaction,fragment
R1321	T2306	T2305	compound	chain,reaction
R1322	T2307	T2305	punct	(,reaction
R1323	T2308	T2305	appos	PCR,reaction
R1324	T2309	T2303	punct	),performed
R1325	T2310	T2303	auxpass	was,performed
R1326	T2311	T2303	advcl	using,performed
R1327	T2312	T2313	nmod	Expanded,system
R1328	T2313	T2317	compound	system,kit
R1329	T2314	T2315	amod	Long,PCR
R1330	T2315	T2313	compound	PCR,system
R1331	T2316	T2315	compound	Template,PCR
R1332	T2317	T2311	dobj	kit,using
R1333	T2318	T2319	punct	(,Science
R1334	T2319	T2317	parataxis	Science,kit
R1335	T2320	T2319	compound	Roche,Science
R1336	T2321	T2319	compound	Applied,Science
R1337	T2322	T2319	punct	", ",Science
R1338	T2323	T2319	npadvmod	Indianapolis,Science
R1339	T2324	T2319	punct	", ",Science
R1340	T2325	T2319	npadvmod	IA,Science
R1341	T2326	T2319	punct	", ",Science
R1342	T2327	T2319	npadvmod	USA,Science
R1343	T2328	T2319	punct	),Science
R1344	T2329	T2303	punct	.,performed
R1345	T2331	T2332	det	An,fragment
R1346	T2332	T2342	nsubjpass	fragment,inserted
R1347	T2333	T2334	advmod	approximately,4.5
R1348	T2334	T2335	nummod	4.5,kb
R1349	T2335	T2332	nmod	kb,fragment
R1350	T2336	T2332	punct	',fragment
R1351	T2337	T2338	amod	long,arm
R1352	T2338	T2332	nmod	arm,fragment
R1353	T2339	T2338	punct	-,arm
R1354	T2340	T2332	punct	',fragment
R1355	T2341	T2332	amod	genomic,fragment
R1356	T2343	T2332	acl	containing,fragment
R1357	T2344	T2345	amod	partial,exon
R1358	T2345	T2343	dobj	exon,containing
R1359	T2346	T2345	nummod	1,exon
R1360	T2347	T2345	prep	to,exon
R1361	T2348	T2349	amod	partial,exon
R1362	T2349	T2347	pobj	exon,to
R1363	T2350	T2349	nummod	3,exon
R1364	T2351	T2342	auxpass	was,inserted
R1365	T2352	T2342	prep	into,inserted
R1366	T2353	T2354	det	the,A
R1367	T2354	T2352	pobj	A,into
R1368	T2355	T2354	nmod	Pml,A
R1369	T2356	T2355	nummod	I,Pml
R1370	T2357	T2358	npadvmod	restriction,cloning
R1371	T2358	T2354	amod	cloning,A
R1372	T2359	T2358	punct	-,cloning
R1373	T2360	T2354	compound	site,A
R1374	T2361	T2354	prep	of,A
R1375	T2362	T2363	compound	OSDUPDEL,vector
R1376	T2363	T2361	pobj	vector,of
R1377	T2364	T2342	punct	.,inserted
R1378	T2366	T2367	det	The,fragment
R1379	T2367	T2374	nsubjpass	fragment,cloned
R1380	T2368	T2367	punct	',fragment
R1381	T2369	T2370	nmod	short,arm
R1382	T2370	T2367	nmod	arm,fragment
R1383	T2371	T2370	punct	-,arm
R1384	T2372	T2367	punct	',fragment
R1385	T2373	T2367	amod	genomic,fragment
R1386	T2375	T2367	punct	", ",fragment
R1387	T2376	T2367	acl	containing,fragment
R1388	T2377	T2378	amod	partial,exon
R1389	T2378	T2376	dobj	exon,containing
R1390	T2379	T2378	nummod	4,exon
R1391	T2380	T2378	prep	to,exon
R1392	T2381	T2382	amod	partial,exon
R1393	T2382	T2380	pobj	exon,to
R1394	T2383	T2382	nummod	6,exon
R1395	T2384	T2374	punct	", ",cloned
R1396	T2385	T2374	auxpass	was,cloned
R1397	T2386	T2374	prep	into,cloned
R1398	T2387	T2388	det	the,Kpn
R1399	T2388	T2386	pobj	Kpn,into
R1400	T2389	T2390	compound	Not,I
R1401	T2390	T2388	compound	I,Kpn
R1402	T2391	T2388	punct	-,Kpn
R1403	T2392	T2388	nummod	I,Kpn
R1404	T2393	T2388	prep	of,Kpn
R1405	T2394	T2395	amod	cloning,B
R1406	T2395	T2393	pobj	B,of
R1407	T2396	T2395	compound	site,B
R1408	T2397	T2374	punct	.,cloned
R1409	T2399	T2400	amod	Homologous,targeting
R1410	T2400	T2401	nsubj	targeting,result
R1411	T2402	T2401	aux	would,result
R1412	T2403	T2401	prep	in,result
R1413	T2404	T2405	amod	complete,intron
R1414	T2405	T2403	pobj	intron,in
R1415	T2406	T2405	nummod	3,intron
R1416	T2407	T2405	punct	", ",intron
R1417	T2408	T2409	amod	partial,exon
R1418	T2409	T2405	conj	exon,intron
R1419	T2410	T2409	nummod	3,exon
R1420	T2411	T2409	cc	and,exon
R1421	T2412	T2413	amod	partial,exon
R1422	T2413	T2414	nmod	exon,replacement
R1423	T2414	T2409	conj	replacement,exon
R1424	T2415	T2413	nummod	4,exon
R1425	T2416	T2405	prep	by,intron
R1426	T2417	T2418	det	the,cassette
R1427	T2418	T2416	pobj	cassette,by
R1428	T2419	T2418	nmod	neomycin,cassette
R1429	T2420	T2419	punct	-,neomycin
R1430	T2421	T2419	amod	resistance,neomycin
R1431	T2422	T2401	punct	", ",result
R1432	T2423	T2401	advcl	resulting,result
R1433	T2424	T2423	prep	in,resulting
R1434	T2425	T2426	det	the,disruption
R1435	T2426	T2424	pobj	disruption,in
R1436	T2427	T2426	prep	of,disruption
R1437	T2428	T2429	det	the,motif
R1438	T2429	T2427	pobj	motif,of
R1439	T2430	T2431	compound	ABC,A
R1440	T2431	T2429	compound	A,motif
R1441	T2432	T2431	compound	Walker,A
R1442	T2433	T2434	punct	(,1a
R1443	T2434	T2401	parataxis	1a,result
R1444	T2435	T2434	compound	Figure,1a
R1445	T2436	T2434	punct	),1a
R1446	T2437	T2401	punct	.,result
R1447	T2439	T2440	compound	ES,cells
R1448	T2440	T2441	nsubjpass	cells,electroporated
R1449	T2442	T2443	punct	(,line
R1451	T2444	T2445	nummod	129,SvEvTac
R1452	T2445	T2443	compound	SvEvTac,line
R1453	T2446	T2445	punct	/,SvEvTac
R1454	T2447	T2443	punct	-,line
R1455	T2448	T2443	compound	cell,line
R1456	T2449	T2443	punct	),line
R1457	T2450	T2441	auxpass	were,electroporated
R1458	T2451	T2441	prep	with,electroporated
R1459	T2452	T2453	det	the,DNA
R1460	T2453	T2451	pobj	DNA,with
R1461	T2454	T2455	amod	linearized,vector
R1462	T2455	T2453	compound	vector,DNA
R1463	T2456	T2455	compound	targeting,vector
R1464	T2457	T2441	cc	and,electroporated
R1465	T2458	T2441	conj	cultured,electroporated
R1466	T2459	T2458	prep	on,cultured
R1467	T2460	T2461	amod	sub-confluent,fibroblasts
R1468	T2461	T2459	pobj	fibroblasts,on
R1469	T2462	T2461	amod	embryonic,fibroblasts
R1470	T2463	T2441	punct	.,electroporated
R1471	T2465	T2466	amod	Transfected,colonies
R1472	T2466	T2468	nsubjpass	colonies,selected
R1473	T2467	T2466	compound	cell,colonies
R1474	T2469	T2468	auxpass	were,selected
R1475	T2470	T2468	agent	by,selected
R1476	T2471	T2470	pcomp	culturing,by
R1477	T2472	T2471	dobj	cells,culturing
R1478	T2473	T2471	prep	in,culturing
R1479	T2474	T2473	pobj	medium,in
R1480	T2475	T2474	acl	containing,medium
R1481	T2476	T2477	nummod	200,μg
R1482	T2477	T2478	nmod	μg,G418
R1483	T2478	T2475	dobj	G418,containing
R1484	T2479	T2480	punct	/,mL
R1485	T2480	T2477	prep	mL,μg
R1486	T2481	T2482	punct	(,Technologies
R1487	T2482	T2478	parataxis	Technologies,G418
R1488	T2483	T2482	compound	Life,Technologies
R1489	T2484	T2482	punct	", ",Technologies
R1490	T2485	T2482	npadvmod	Rockville,Technologies
R1491	T2486	T2482	punct	", ",Technologies
R1492	T2487	T2482	npadvmod	MD,Technologies
R1493	T2488	T2482	punct	", ",Technologies
R1494	T2489	T2482	npadvmod	USA,Technologies
R1495	T2490	T2482	punct	),Technologies
R1496	T2491	T2468	punct	.,selected
R1497	T2493	T2494	det	The,cells
R1498	T2494	T2496	nsubjpass	cells,screened
R1499	T2495	T2494	compound	ES,cells
R1500	T2497	T2496	auxpass	were,screened
R1501	T2498	T2496	prep	for,screened
R1502	T2499	T2500	amod	homologous,recombination
R1503	T2500	T2498	pobj	recombination,for
R1504	T2501	T2496	prep	by,screened
R1505	T2502	T2501	pobj	PCR,by
R1506	T2503	T2496	prep	with,screened
R1507	T2504	T2505	det	the,primer
R1508	T2505	T2503	pobj	primer,with
R1509	T2506	T2505	amod	forward,primer
R1510	T2507	T2505	punct	", ",primer
R1511	T2508	T2505	appos	neoF,primer
R1512	T2509	T2508	punct	(,neoF
R1513	T2510	T2511	nummod	5,GGGTCGTTTGTTCGGATCAA
R1514	T2511	T2508	appos	GGGTCGTTTGTTCGGATCAA,neoF
R1515	T2512	T2511	punct	',GGGTCGTTTGTTCGGATCAA
R1516	T2513	T2511	punct	-,GGGTCGTTTGTTCGGATCAA
R1517	T2514	T2511	punct	-,GGGTCGTTTGTTCGGATCAA
R1518	T2515	T2511	nummod	3,GGGTCGTTTGTTCGGATCAA
R1519	T2516	T2511	punct	',GGGTCGTTTGTTCGGATCAA
R1520	T2517	T2508	punct	),neoF
R1521	T2518	T2508	prep	from,neoF
R1522	T2519	T2520	compound	neo,cassette
R1523	T2520	T2518	pobj	cassette,from
R1524	T2521	T2505	punct	", ",primer
R1525	T2522	T2505	cc	and,primer
R1526	T2523	T2524	amod	reverse,primer
R1527	T2524	T2525	compound	primer,6R
R1528	T2525	T2505	conj	6R,primer
R1529	T2526	T2525	compound	intron,6R
R1530	T2527	T2525	punct	(,6R
R1531	T2528	T2529	nummod	5,ACCAGTTGGTCCTAGCTCGA
R1532	T2529	T2525	appos	ACCAGTTGGTCCTAGCTCGA,6R
R1533	T2530	T2528	punct	',5
R1534	T2531	T2529	punct	-,ACCAGTTGGTCCTAGCTCGA
R1535	T2532	T2529	punct	-,ACCAGTTGGTCCTAGCTCGA
R1536	T2533	T2529	nummod	3,ACCAGTTGGTCCTAGCTCGA
R1537	T2534	T2529	punct	',ACCAGTTGGTCCTAGCTCGA
R1538	T2535	T2525	punct	),6R
R1539	T2536	T2525	punct	", ",6R
R1540	T2537	T2538	dep	which,located
R1541	T2538	T2525	relcl	located,6R
R1542	T2539	T2538	auxpass	is,located
R1543	T2540	T2538	prep	outside,located
R1544	T2541	T2542	det	the,construct
R1545	T2542	T2540	pobj	construct,outside
R1546	T2543	T2542	amod	targeting,construct
R1547	T2544	T2538	prep	in,located
R1548	T2545	T2546	compound	mouse,intron
R1549	T2546	T2544	pobj	intron,in
R1550	T2547	T2546	compound	Abcg8,intron
R1551	T2548	T2546	nummod	6,intron
R1552	T2549	T2496	punct	.,screened
R1553	T2551	T2552	advmod	Positively,targeted
R1554	T2552	T2553	amod	targeted,clones
R1555	T2553	T2556	nsubjpass	clones,confirmed
R1556	T2554	T2555	compound	ES,cell
R1557	T2555	T2553	compound	cell,clones
R1558	T2557	T2556	auxpass	were,confirmed
R1559	T2558	T2556	prep	by,confirmed
R1560	T2559	T2560	compound	Southern,blotting
R1561	T2560	T2558	pobj	blotting,by
R1562	T2561	T2556	punct	", ",confirmed
R1563	T2562	T2556	advcl	using,confirmed
R1564	T2563	T2564	compound	ES,cell
R1565	T2564	T2565	compound	cell,DNA
R1566	T2565	T2562	dobj	DNA,using
R1567	T2566	T2565	acl	digested,DNA
R1568	T2567	T2566	prep	by,digested
R1569	T2568	T2567	pobj	BamHI,by
R1570	T2569	T2568	cc	and,BamHI
R1571	T2570	T2571	det	a,probe
R1572	T2571	T2568	conj	probe,BamHI
R1573	T2572	T2571	acl	comprising,probe
R1574	T2573	T2572	prep	of,comprising
R1575	T2574	T2575	det	a,fragment
R1576	T2575	T2573	pobj	fragment,of
R1577	T2576	T2577	nummod	562,bp
R1578	T2577	T2575	compound	bp,fragment
R1579	T2578	T2575	compound	PCR,fragment
R1580	T2579	T2575	prep	from,fragment
R1581	T2580	T2581	amod	partial,intron
R1582	T2581	T2579	pobj	intron,from
R1583	T2582	T2581	nummod	2,intron
R1584	T2583	T2581	cc	and,intron
R1585	T2584	T2581	conj	exon,intron
R1586	T2585	T2584	nummod	3,exon
R1587	T2586	T2581	prep	of,intron
R1588	T2587	T2588	compound	Abcg8,gene
R1589	T2588	T2586	pobj	gene,of
R1590	T2589	T2556	punct	.,confirmed
R1591	T2591	T2592	advmod	Positively,targeted
R1592	T2592	T2593	amod	targeted,cells
R1593	T2593	T2595	nsubjpass	cells,microinjected
R1594	T2594	T2593	compound	ES,cells
R1595	T2596	T2595	auxpass	were,microinjected
R1596	T2597	T2595	prep	into,microinjected
R1597	T2598	T2599	compound	C57BL6,blastocysts
R1598	T2599	T2597	pobj	blastocysts,into
R1599	T2600	T2595	cc	and,microinjected
R1600	T2601	T2595	conj	transplanted,microinjected
R1601	T2602	T2601	prep	into,transplanted
R1602	T2603	T2604	amod	pseudopregnant,recipients
R1603	T2604	T2602	pobj	recipients,into
R1604	T2605	T2595	punct	.,microinjected
R1605	T2607	T2608	nummod	Five,mice
R1606	T2608	T2611	nsubjpass	mice,isolated
R1607	T2609	T2610	advmod	highly,chimeric
R1608	T2610	T2608	amod	chimeric,mice
R1609	T2612	T2613	punct	(,males
R1611	T2614	T2615	amod	agouti,color
R1612	T2615	T2613	dep	color,males
R1613	T2616	T2615	compound	coat,color
R1614	T2617	T2613	punct	", ",males
R1615	T2618	T2613	nummod	three,males
R1616	T2619	T2613	cc	and,males
R1617	T2620	T2621	nummod	two,females
R1618	T2621	T2613	conj	females,males
R1619	T2622	T2613	punct	),males
R1620	T2623	T2611	auxpass	were,isolated
R1621	T2624	T2611	cc	and,isolated
R1622	T2625	T2611	conj	bred,isolated
R1623	T2626	T2625	prep	with,bred
R1624	T2627	T2628	compound	C57BL,6J
R1625	T2628	T2630	compound	6J,mice
R1626	T2629	T2628	punct	/,6J
R1627	T2630	T2626	pobj	mice,with
R1628	T2631	T2632	aux	to,generate
R1629	T2632	T2625	advcl	generate,bred
R1630	T2633	T2634	compound	germ,line
R1631	T2634	T2636	compound	line,transmission
R1632	T2635	T2634	punct	-,line
R1633	T2636	T2637	npadvmod	transmission,heterozygous
R1634	T2637	T2638	amod	heterozygous,mice
R1635	T2638	T2632	dobj	mice,generate
R1636	T2639	T2638	prep	of,mice
R1637	T2640	T2641	compound	Abcg8,gene
R1638	T2641	T2642	compound	gene,disruption
R1639	T2642	T2639	pobj	disruption,of
R1640	T2643	T2611	punct	.,isolated
R1641	T2645	T2646	amod	Heterozygous,mice
R1642	T2646	T2648	nsubjpass	mice,crossed
R1643	T2647	T2646	compound	offspring,mice
R1644	T2649	T2648	auxpass	were,crossed
R1645	T2650	T2648	advmod	back,crossed
R1646	T2651	T2648	punct	-,crossed
R1647	T2652	T2648	prep	to,crossed
R1648	T2653	T2654	compound	C57BL,6J
R1649	T2654	T2656	compound	6J,mice
R1650	T2655	T2654	punct	/,6J
R1651	T2656	T2652	pobj	mice,to
R1652	T2657	T2658	punct	(,5
R1653	T2658	T2656	parataxis	5,mice
R1654	T2659	T2658	nsubj	n,5
R1655	T2660	T2658	punct	>,5
R1656	T2661	T2658	punct	),5
R1657	T2662	T2663	aux	to,produce
R1658	T2663	T2648	advcl	produce,crossed
R1659	T2664	T2665	amod	disrupted,line
R1660	T2665	T2663	dobj	line,produce
R1661	T2666	T2648	cc	and,crossed
R1662	T2667	T2648	conj	inter-crossed,crossed
R1663	T2668	T2669	aux	to,generate
R1664	T2669	T2667	advcl	generate,inter-crossed
R1665	T2670	T2671	compound	knockout,mice
R1666	T2671	T2669	dobj	mice,generate
R1667	T2672	T2648	punct	.,crossed
R1668	T2716	T2715	cc	and,Animals
R1669	T2717	T2715	conj	diets,Animals
R1670	T2719	T2720	det	All,mice
R1671	T2720	T2721	nsubjpass	mice,maintained
R1672	T2722	T2721	auxpass	were,maintained
R1673	T2723	T2721	prep	on,maintained
R1674	T2724	T2725	det	a,chow
R1675	T2725	T2723	pobj	chow,on
R1676	T2726	T2725	amod	standard,chow
R1677	T2727	T2725	compound	rodent,chow
R1678	T2728	T2725	punct	(,chow
R1679	T2729	T2730	compound	Harlan,Teklad
R1680	T2730	T2731	compound	Teklad,LM
R1681	T2731	T2725	appos	LM,chow
R1682	T2732	T2733	compound	mouse,rat
R1683	T2733	T2731	compound	rat,LM
R1684	T2734	T2733	punct	/,rat
R1685	T2735	T2731	compound	diet,LM
R1686	T2736	T2731	punct	-,LM
R1687	T2737	T2731	nummod	485,LM
R1688	T2738	T2725	punct	),chow
R1689	T2739	T2740	dep	which,contained
R1690	T2740	T2725	relcl	contained,chow
R1691	T2741	T2742	nummod	61,mg
R1692	T2742	T2743	nmod	mg,cholesterol
R1693	T2743	T2740	dobj	cholesterol,contained
R1694	T2744	T2745	punct	/,kg
R1695	T2745	T2742	prep	kg,mg
R1696	T2746	T2743	punct	", ",cholesterol
R1697	T2747	T2748	nummod	31,mg
R1698	T2748	T2749	nmod	mg,campesterol
R1699	T2749	T2743	conj	campesterol,cholesterol
R1700	T2750	T2751	punct	/,kg
R1701	T2751	T2748	prep	kg,mg
R1702	T2752	T2749	punct	", ",campesterol
R1703	T2753	T2749	cc	and,campesterol
R1704	T2754	T2755	nummod	105,mg
R1705	T2755	T2756	nmod	mg,sitosterol
R1706	T2756	T2749	conj	sitosterol,campesterol
R1707	T2757	T2758	punct	/,kg
R1708	T2758	T2755	prep	kg,mg
R1709	T2759	T2721	punct	", ",maintained
R1710	T2760	T2721	conj	given,maintained
R1711	T2761	T2762	amod	free,access
R1712	T2762	T2760	dobj	access,given
R1713	T2763	T2762	prep	to,access
R1714	T2764	T2763	pobj	water,to
R1715	T2765	T2760	cc	and,given
R1716	T2766	T2760	conj	maintained,given
R1717	T2767	T2766	prep	at,maintained
R1718	T2768	T2769	nummod	25,°C
R1719	T2769	T2767	pobj	°C,at
R1720	T2770	T2766	prep	with,maintained
R1721	T2771	T2772	det	a,cycle
R1722	T2772	T2770	pobj	cycle,with
R1723	T2773	T2774	nummod	12,h
R1724	T2774	T2772	nmod	h,cycle
R1725	T2775	T2774	punct	-,h
R1726	T2776	T2774	amod	light,h
R1727	T2777	T2774	punct	", ",h
R1728	T2778	T2779	nummod	12,h
R1729	T2779	T2774	appos	h,h
R1730	T2780	T2779	punct	-,h
R1731	T2781	T2779	amod	dark,h
R1732	T2782	T2721	punct	.,maintained
R1733	T2784	T2785	det	All,animals
R1734	T2785	T2786	nsubjpass	animals,housed
R1735	T2787	T2786	auxpass	were,housed
R1736	T2788	T2786	prep	in,housed
R1737	T2789	T2790	det	the,facilities
R1738	T2790	T2788	pobj	facilities,in
R1739	T2791	T2790	prep	for,facilities
R1740	T2792	T2793	compound	laboratory,animals
R1741	T2793	T2791	pobj	animals,for
R1742	T2794	T2790	acl	provided,facilities
R1743	T2795	T2794	agent	by,provided
R1744	T2796	T2797	det	the,Division
R1745	T2797	T2795	pobj	Division,by
R1746	T2798	T2797	prep	of,Division
R1747	T2799	T2800	compound	Laboratory,Animal
R1748	T2800	T2801	compound	Animal,Resources
R1749	T2801	T2798	pobj	Resources,of
R1750	T2802	T2797	appos	ACLAM,Division
R1751	T2803	T2786	punct	.,housed
R1752	T2805	T2806	det	The,Committee
R1753	T2806	T2813	nsubj	Committee,approved
R1754	T2807	T2808	nmod	Institutional,Animal
R1755	T2808	T2806	nmod	Animal,Committee
R1756	T2809	T2808	appos	Care,Animal
R1757	T2810	T2809	cc	and,Care
R1758	T2811	T2809	conj	Research,Care
R1759	T2812	T2806	compound	Advisory,Committee
R1760	T2814	T2806	prep	at,Committee
R1761	T2815	T2816	compound	Medical,University
R1762	T2816	T2814	pobj	University,at
R1763	T2817	T2816	prep	of,University
R1764	T2818	T2819	compound	South,Carolina
R1765	T2819	T2817	pobj	Carolina,of
R1766	T2820	T2821	det	all,experiments
R1767	T2821	T2813	dobj	experiments,approved
R1768	T2822	T2813	punct	.,approved
R1769	T2919	T2918	prep	by,Genotyping
R1770	T2920	T2919	pobj	PCR,by
R1771	T2922	T2923	compound	Mouse,tail
R1772	T2923	T2924	compound	tail,DNA
R1773	T2924	T2925	nsubjpass	DNA,isolated
R1774	T2926	T2925	auxpass	was,isolated
R1775	T2927	T2925	cc	and,isolated
R1776	T2928	T2925	conj	purified,isolated
R1777	T2929	T2928	prep	by,purified
R1778	T2930	T2929	pcomp	cutting,by
R1779	T2931	T2932	advmod	approximately,0.5
R1780	T2932	T2933	nummod	0.5,cm
R1781	T2933	T2930	dobj	cm,cutting
R1782	T2934	T2933	prep	of,cm
R1783	T2935	T2936	compound	mouse,tail
R1784	T2936	T2934	pobj	tail,of
R1785	T2937	T2930	cc	and,cutting
R1786	T2938	T2930	conj	digesting,cutting
R1787	T2939	T2938	dobj	it,digesting
R1788	T2940	T2938	prep	in,digesting
R1789	T2941	T2942	nummod	500,μl
R1790	T2942	T2943	compound	μl,buffer
R1791	T2943	T2940	pobj	buffer,in
R1792	T2944	T2943	compound	lysis,buffer
R1793	T2945	T2946	punct	(,HCl
R1794	T2946	T2943	parataxis	HCl,buffer
R1795	T2947	T2948	nummod	50,mM
R1796	T2948	T2946	compound	mM,HCl
R1797	T2949	T2946	compound	Tris,HCl
R1798	T2950	T2946	punct	-,HCl
R1799	T2951	T2946	punct	", ",HCl
R1800	T2952	T2946	npadvmod	pH,HCl
R1801	T2953	T2952	nummod	8.0,pH
R1802	T2954	T2946	punct	", ",HCl
R1803	T2955	T2956	nummod	100,mM
R1804	T2956	T2957	compound	mM,EDTA
R1805	T2957	T2946	appos	EDTA,HCl
R1806	T2958	T2946	punct	", ",HCl
R1807	T2959	T2960	nummod	125,mM
R1808	T2960	T2961	compound	mM,NaCl
R1809	T2961	T2946	appos	NaCl,HCl
R1810	T2962	T2946	punct	", ",HCl
R1811	T2963	T2964	nummod	1,%
R1812	T2964	T2965	compound	%,SDS
R1813	T2965	T2946	appos	SDS,HCl
R1814	T2966	T2946	punct	", ",HCl
R1815	T2967	T2968	nummod	200,μg
R1816	T2968	T2969	compound	μg,K
R1817	T2969	T2946	appos	K,HCl
R1818	T2970	T2969	compound	Proteinase,K
R1819	T2971	T2946	punct	),HCl
R1820	T2972	T2938	prep	with,digesting
R1821	T2973	T2972	pobj	rocking,with
R1822	T2974	T2973	advmod	overnight,rocking
R1823	T2975	T2973	prep	at,rocking
R1824	T2976	T2977	nummod	55,°C
R1825	T2977	T2975	pobj	°C,at
R1826	T2978	T2925	punct	.,isolated
R1827	T2980	T2981	nummod	200,μl
R1828	T2981	T2982	nmod	μl,NaCl
R1829	T2982	T2984	nsubjpass	NaCl,added
R1830	T2983	T2982	amod	saturated,NaCl
R1831	T2985	T2984	auxpass	was,added
R1832	T2986	T2984	prep	into,added
R1833	T2987	T2988	amod	digested,solution
R1834	T2988	T2986	pobj	solution,into
R1835	T2989	T2984	prep	with,added
R1836	T2990	T2991	amod	vigorous,shaking
R1837	T2991	T2989	pobj	shaking,with
R1838	T2992	T2991	prep	for,shaking
R1839	T2993	T2994	quantmod	at,60
R1840	T2994	T2996	nummod	60,seconds
R1841	T2995	T2993	advmod	least,at
R1842	T2996	T2992	pobj	seconds,for
R1843	T2997	T2984	punct	.,added
R1844	T2999	T3000	amod	Mixed,solution
R1845	T3000	T3001	nsubjpass	solution,spun
R1846	T3002	T3001	auxpass	was,spun
R1847	T3003	T3001	prt	down,spun
R1848	T3004	T3001	prep	at,spun
R1849	T3005	T3006	amod	maximum,speed
R1850	T3006	T3004	pobj	speed,at
R1851	T3007	T3001	prep	in,spun
R1852	T3008	T3009	amod	top,centrifuge
R1853	T3009	T3007	pobj	centrifuge,in
R1854	T3010	T3009	compound	micro,centrifuge
R1855	T3011	T3009	punct	-,centrifuge
R1856	T3012	T3001	prep	for,spun
R1857	T3013	T3014	nummod	20,min
R1858	T3014	T3012	pobj	min,for
R1859	T3015	T3001	punct	.,spun
R1860	T3017	T3018	det	The,supernatant
R1861	T3018	T3019	nsubjpass	supernatant,transferred
R1862	T3020	T3019	auxpass	was,transferred
R1863	T3021	T3019	prep	to,transferred
R1864	T3022	T3023	det	a,tube
R1865	T3023	T3021	pobj	tube,to
R1866	T3024	T3023	amod	new,tube
R1867	T3025	T3019	prep	with,transferred
R1868	T3026	T3027	amod	equal,EtOH
R1869	T3027	T3025	pobj	EtOH,with
R1870	T3028	T3027	nmod	volume,EtOH
R1871	T3029	T3030	nummod	100,%
R1872	T3030	T3027	compound	%,EtOH
R1873	T3031	T3019	cc	and,transferred
R1874	T3032	T3019	conj	mixed,transferred
R1875	T3033	T3032	prep	by,mixed
R1876	T3034	T3033	pobj	inversion,by
R1877	T3035	T3019	punct	.,transferred
R1878	T3037	T3038	det	The,pellet
R1879	T3038	T3040	nsubjpass	pellet,washed
R1880	T3039	T3038	compound	DNA,pellet
R1881	T3041	T3040	auxpass	was,washed
R1882	T3042	T3040	prep	in,washed
R1883	T3043	T3044	nummod	70,%
R1884	T3044	T3045	compound	%,EtOH
R1885	T3045	T3042	pobj	EtOH,in
R1886	T3046	T3040	cc	and,washed
R1887	T3047	T3040	conj	resuspended,washed
R1888	T3048	T3047	prep	in,resuspended
R1889	T3049	T3050	quantmod	100,150
R1890	T3050	T3052	nummod	150,μl
R1891	T3051	T3050	punct	–,150
R1892	T3052	T3053	compound	μl,buffer
R1893	T3053	T3048	pobj	buffer,in
R1894	T3054	T3053	compound	TE,buffer
R1895	T3055	T3040	punct	.,washed
R1896	T3057	T3058	compound	UV,spectrophotometry
R1897	T3058	T3059	nsubjpass	spectrophotometry,used
R1898	T3060	T3058	cc	and,spectrophotometry
R1899	T3061	T3058	conj	electrophoresis,spectrophotometry
R1900	T3062	T3059	auxpass	were,used
R1901	T3063	T3064	aux	to,analyze
R1902	T3064	T3059	advcl	analyze,used
R1903	T3065	T3066	det	the,quality
R1904	T3066	T3064	dobj	quality,analyze
R1905	T3067	T3066	cc	and,quality
R1906	T3068	T3066	conj	quantity,quality
R1907	T3069	T3066	prep	of,quality
R1908	T3070	T3071	det	the,DNA
R1909	T3071	T3069	pobj	DNA,of
R1910	T3072	T3071	amod	genomic,DNA
R1911	T3073	T3059	punct	.,used
R1912	T3075	T3076	nsubjpass	Genotyping,performed
R1913	T3077	T3076	auxpass	was,performed
R1914	T3078	T3076	agent	by,performed
R1915	T3079	T3080	amod	multiplex,reaction
R1916	T3080	T3078	pobj	reaction,by
R1917	T3081	T3080	compound	PCR,reaction
R1918	T3082	T3076	advcl	using,performed
R1919	T3083	T3084	nummod	three,primers
R1920	T3084	T3082	dobj	primers,using
R1921	T3085	T3084	amod	separate,primers
R1922	T3086	T3084	punct	(,primers
R1923	T3087	T3088	nummod	two,primers
R1924	T3088	T3084	appos	primers,primers
R1925	T3089	T3088	nmod	Abcg8,primers
R1926	T3090	T3091	npadvmod	gene,specific
R1927	T3091	T3088	amod	specific,primers
R1928	T3092	T3088	punct	", ",primers
R1929	T3093	T3094	nmod	mg8,in3F
R1930	T3094	T3096	nmod	in3F,CCCAATGATGAATGAGACGCT
R1931	T3095	T3094	punct	-,in3F
R1932	T3096	T3088	conj	CCCAATGATGAATGAGACGCT,primers
R1933	T3097	T3096	nummod	5,CCCAATGATGAATGAGACGCT
R1934	T3098	T3097	punct	',5
R1935	T3099	T3096	punct	-,CCCAATGATGAATGAGACGCT
R1936	T3100	T3096	punct	-,CCCAATGATGAATGAGACGCT
R1937	T3101	T3096	nummod	3,CCCAATGATGAATGAGACGCT
R1938	T3102	T3096	punct	',CCCAATGATGAATGAGACGCT
R1939	T3103	T3096	punct	", ",CCCAATGATGAATGAGACGCT
R1940	T3104	T3105	nmod	mg8,R9
R1941	T3105	T3107	nmod	R9,TTTGTCACGCTGGGCCTGG
R1942	T3106	T3105	punct	-,R9
R1943	T3107	T3096	appos	TTTGTCACGCTGGGCCTGG,CCCAATGATGAATGAGACGCT
R1944	T3108	T3107	nummod	5,TTTGTCACGCTGGGCCTGG
R1945	T3109	T3108	punct	',5
R1946	T3110	T3107	punct	-,TTTGTCACGCTGGGCCTGG
R1947	T3111	T3107	punct	-,TTTGTCACGCTGGGCCTGG
R1948	T3112	T3107	nummod	3,TTTGTCACGCTGGGCCTGG
R1949	T3113	T3107	punct	',TTTGTCACGCTGGGCCTGG
R1950	T3114	T3096	cc	and,CCCAATGATGAATGAGACGCT
R1951	T3115	T3096	conj	neoF,CCCAATGATGAATGAGACGCT
R1952	T3116	T3115	prep	for,neoF
R1953	T3117	T3116	pobj	identification,for
R1954	T3118	T3117	prep	of,identification
R1955	T3119	T3120	compound	Abcg8,status
R1956	T3120	T3118	pobj	status,of
R1957	T3121	T3120	compound	target,status
R1958	T3122	T3076	punct	),performed
R1959	T3123	T3076	punct	.,performed
R1960	T3125	T3126	det	The,reaction
R1961	T3126	T3128	nsubj	reaction,consisted
R1962	T3127	T3126	compound	PCR,reaction
R1963	T3129	T3128	prep	of,consisted
R1964	T3130	T3131	nmod	1x,buffer
R1965	T3131	T3133	nmod	buffer,μM
R1966	T3132	T3131	nmod	PCR,buffer
R1967	T3133	T3129	pobj	μM,of
R1968	T3134	T3135	punct	(,MgCl2
R1969	T3135	T3131	parataxis	MgCl2,buffer
R1970	T3136	T3137	nummod	1.5,mM
R1971	T3137	T3135	compound	mM,MgCl2
R1972	T3138	T3135	punct	", ",MgCl2
R1973	T3139	T3140	nummod	16,mM
R1974	T3140	T3141	nmod	mM,2SO4
R1975	T3141	T3135	appos	2SO4,MgCl2
R1976	T3142	T3143	punct	(,NH4
R1977	T3143	T3141	parataxis	NH4,2SO4
R1978	T3144	T3143	punct	),NH4
R1979	T3145	T3135	punct	", ",MgCl2
R1980	T3146	T3147	nummod	0.1,mM
R1981	T3147	T3148	compound	mM,dNTPs
R1982	T3148	T3135	appos	dNTPs,MgCl2
R1983	T3149	T3135	punct	", ",MgCl2
R1984	T3150	T3151	nummod	67,mM
R1985	T3151	T3152	compound	mM,HCl
R1986	T3152	T3135	appos	HCl,MgCl2
R1987	T3153	T3152	compound	Tris,HCl
R1988	T3154	T3152	punct	-,HCl
R1989	T3155	T3156	punct	(,pH
R1990	T3156	T3152	parataxis	pH,HCl
R1991	T3157	T3156	nummod	8.8,pH
R1992	T3158	T3156	punct	),pH
R1993	T3159	T3135	punct	),MgCl2
R1994	T3160	T3133	nummod	1,μM
R1995	T3161	T3133	prep	of,μM
R1996	T3162	T3163	det	each,primer
R1997	T3163	T3161	pobj	primer,of
R1998	T3164	T3133	punct	", ",μM
R1999	T3165	T3133	conj	water,μM
R2000	T3166	T3165	cc	and,water
R2001	T3167	T3168	nummod	1.0,units
R2002	T3168	T3165	conj	units,water
R2003	T3169	T3168	prep	of,units
R2004	T3170	T3171	compound	Taq,polymerase
R2005	T3171	T3169	pobj	polymerase,of
R2006	T3172	T3128	punct	.,consisted
R2007	T3174	T3175	det	The,reactions
R2008	T3175	T3176	nsubjpass	reactions,cycled
R2009	T3177	T3176	auxpass	were,cycled
R2010	T3178	T3179	mark	as,follows
R2011	T3179	T3176	advcl	follows,cycled
R2012	T3180	T3176	punct	: ,cycled
R2013	T3181	T3182	nummod	94,°C
R2014	T3182	T3176	dep	°C,cycled
R2015	T3183	T3182	prep	for,°C
R2016	T3184	T3185	nummod	30,sec
R2017	T3185	T3183	pobj	sec,for
R2018	T3186	T3182	punct	", ",°C
R2019	T3187	T3188	nummod	60,°C
R2020	T3188	T3182	appos	°C,°C
R2021	T3189	T3188	prep	for,°C
R2022	T3190	T3191	nummod	30,sec
R2023	T3191	T3189	pobj	sec,for
R2024	T3192	T3182	punct	", ",°C
R2025	T3193	T3194	nummod	72,°C
R2026	T3194	T3182	appos	°C,°C
R2027	T3195	T3194	prep	for,°C
R2028	T3196	T3197	nummod	1,min
R2029	T3197	T3195	pobj	min,for
R2030	T3198	T3182	prep	for,°C
R2031	T3199	T3200	nummod	35,cycles
R2032	T3200	T3198	pobj	cycles,for
R2033	T3201	T3176	punct	.,cycled
R2034	T3203	T3204	det	The,products
R2035	T3204	T3206	nsubjpass	products,identified
R2036	T3205	T3204	compound	PCR,products
R2037	T3207	T3206	auxpass	were,identified
R2038	T3208	T3206	prep	by,identified
R2039	T3209	T3208	pobj	electrophoresis,by
R2040	T3210	T3206	punct	.,identified
R2041	T3239	T3240	compound	Southern,blot
R2042	T3240	T3241	compound	blot,analysis
R2043	T3243	T3244	aux	To,confirm
R2044	T3244	T3245	advcl	confirm,digested
R2045	T3246	T3247	compound	PCR,results
R2046	T3247	T3244	dobj	results,confirm
R2047	T3248	T3245	punct	", ",digested
R2048	T3249	T3245	nsubjpass	DNAs,digested
R2049	T3250	T3245	auxpass	were,digested
R2050	T3251	T3245	prep	with,digested
R2051	T3252	T3253	compound	Bam,HI
R2052	T3253	T3251	pobj	HI,with
R2053	T3254	T3245	punct	", ",digested
R2054	T3255	T3245	conj	subjected,digested
R2055	T3256	T3255	prep	to,subjected
R2056	T3257	T3256	pobj	electrophoresis,to
R2057	T3258	T3255	prep	in,subjected
R2058	T3259	T3260	det	a,gel
R2059	T3260	T3258	pobj	gel,in
R2060	T3261	T3262	nummod	0.6,%
R2061	T3262	T3260	compound	%,gel
R2062	T3263	T3260	compound	agarose,gel
R2063	T3264	T3255	cc	and,subjected
R2064	T3265	T3255	conj	transferred,subjected
R2065	T3266	T3265	prep	to,transferred
R2066	T3267	T3268	compound	nylon,membrane
R2067	T3268	T3266	pobj	membrane,to
R2068	T3269	T3245	punct	.,digested
R2069	T3271	T3272	compound	Southern,blot
R2070	T3272	T3273	compound	blot,filters
R2071	T3273	T3274	nsubjpass	filters,hybridized
R2072	T3275	T3274	auxpass	were,hybridized
R2073	T3276	T3274	prep	with,hybridized
R2074	T3277	T3278	det	a,fragment
R2075	T3278	T3276	pobj	fragment,with
R2076	T3279	T3280	nummod	562,bp
R2077	T3280	T3278	compound	bp,fragment
R2078	T3281	T3278	compound	PCR,fragment
R2079	T3282	T3278	prep	from,fragment
R2080	T3283	T3284	amod	partial,intron
R2081	T3284	T3282	pobj	intron,from
R2082	T3285	T3284	nummod	2,intron
R2083	T3286	T3284	cc	and,intron
R2084	T3287	T3284	conj	exon,intron
R2085	T3288	T3287	nummod	3,exon
R2086	T3289	T3284	prep	of,intron
R2087	T3290	T3291	nmod	Abcg8,probe
R2089	T3292	T3293	npadvmod	32P,labeled
R2090	T3293	T3291	amod	labeled,probe
R2091	T3294	T3293	punct	-,labeled
R2092	T3295	T3293	advmod	randomly,labeled
R2093	T3296	T3293	punct	-,labeled
R2094	T3297	T3298	mark	as,described
R2095	T3298	T3274	advcl	described,hybridized
R2096	T3299	T3298	advmod	previously,described
R2097	T3300	T3301	punct	[,22
R2098	T3301	T3274	parataxis	22,hybridized
R2099	T3302	T3301	punct	],22
R2100	T3303	T3274	punct	.,hybridized
R2101	T3344	T3345	compound	Northern,blot
R2102	T3345	T3346	compound	blot,analysis
R2103	T3348	T3349	nsubjpass	Isolation,performed
R2104	T3350	T3348	prep	of,Isolation
R2105	T3351	T3352	npadvmod	liver,intestinal
R2106	T3352	T3354	amod	intestinal,RNA
R2107	T3353	T3352	cc	and,intestinal
R2108	T3354	T3350	pobj	RNA,of
R2109	T3355	T3354	amod	total,RNA
R2110	T3356	T3354	prep	from,RNA
R2111	T3357	T3358	nmod	Abcg8,mice
R2112	T3358	T3356	pobj	mice,from
R2113	T3359	T3357	punct	+,Abcg8
R2114	T3360	T3357	punct	/,Abcg8
R2115	T3361	T3357	punct	+,Abcg8
R2116	T3362	T3357	punct	", ",Abcg8
R2117	T3363	T3357	conj	Abcg8,Abcg8
R2118	T3364	T3363	punct	+,Abcg8
R2119	T3365	T3363	punct	/,Abcg8
R2120	T3366	T3363	punct	-,Abcg8
R2121	T3367	T3363	cc	and,Abcg8
R2122	T3368	T3363	conj	Abcg8,Abcg8
R2123	T3369	T3368	punct	-,Abcg8
R2124	T3370	T3368	punct	/,Abcg8
R2125	T3371	T3368	punct	-,Abcg8
R2126	T3372	T3348	cc	and,Isolation
R2127	T3373	T3374	compound	Northern,blot
R2128	T3374	T3375	compound	blot,analyses
R2129	T3375	T3348	conj	analyses,Isolation
R2130	T3376	T3349	auxpass	were,performed
R2131	T3377	T3378	mark	as,described
R2132	T3378	T3349	advcl	described,performed
R2133	T3379	T3378	advmod	previously,described
R2134	T3380	T3381	punct	[,23
R2135	T3381	T3349	parataxis	23,performed
R2136	T3382	T3381	punct	],23
R2137	T3383	T3349	punct	.,performed
R2138	T3385	T3386	det	The,probe
R2139	T3386	T3387	nsubj	probe,was
R2140	T3388	T3386	prep	for,probe
R2141	T3389	T3388	pobj	Abcg5,for
R2142	T3390	T3391	nummod	1964,bp
R2143	T3391	T3387	attr	bp,was
R2144	T3392	T3393	punct	(,AH011511
R2145	T3393	T3387	parataxis	AH011511,was
R2146	T3394	T3395	nmod	nt,136
R2147	T3395	T3393	dep	136,AH011511
R2148	T3396	T3397	punct	–,2099
R2149	T3397	T3395	prep	2099,136
R2150	T3398	T3393	punct	", ",AH011511
R2151	T3399	T3393	compound	GeneBank™,AH011511
R2152	T3400	T3393	compound	accession,AH011511
R2153	T3401	T3393	compound	no.,AH011511
R2154	T3402	T3393	punct	),AH011511
R2155	T3403	T3387	cc	and,was
R2156	T3404	T3405	det	the,probe
R2157	T3405	T3406	nsubj	probe,was
R2158	T3406	T3387	conj	was,was
R2159	T3407	T3405	prep	for,probe
R2160	T3408	T3407	pobj	Abcg8,for
R2161	T3409	T3410	nummod	2019,bp
R2162	T3410	T3406	attr	bp,was
R2163	T3411	T3412	punct	(,AH011518
R2164	T3412	T3406	parataxis	AH011518,was
R2165	T3413	T3414	nmod	nt,103
R2166	T3414	T3412	dep	103,AH011518
R2167	T3415	T3416	punct	–,2126
R2168	T3416	T3414	prep	2126,103
R2169	T3417	T3412	punct	", ",AH011518
R2170	T3418	T3412	compound	GeneBank™,AH011518
R2171	T3419	T3412	compound	accession,AH011518
R2172	T3420	T3412	compound	no.,AH011518
R2173	T3421	T3412	punct	),AH011518
R2174	T3422	T3406	punct	.,was
R2175	T3461	T3462	amod	Reverse,Transcription
R2176	T3462	T3463	nmod	Transcription,PCR
R2177	T3464	T3462	punct	(,Transcription
R2178	T3465	T3462	appos	RT,Transcription
R2179	T3466	T3463	punct	),PCR
R2180	T3467	T3463	punct	-,PCR
R2181	T3469	T3470	aux	To,confirm
R2182	T3470	T3471	advcl	confirm,performed
R2183	T3472	T3473	mark	that,was
R2184	T3473	T3470	ccomp	was,confirm
R2185	T3474	T3473	expl	there,was
R2186	T3475	T3476	det	no,splicing
R2187	T3476	T3473	attr	splicing,was
R2188	T3477	T3476	amod	alternative,splicing
R2189	T3478	T3476	prep	of,splicing
R2190	T3479	T3480	det	the,allele
R2191	T3480	T3478	pobj	allele,of
R2192	T3481	T3480	amod	disrupted,allele
R2193	T3482	T3471	punct	", ",performed
R2194	T3483	T3484	compound	RT,PCR
R2195	T3484	T3471	nsubjpass	PCR,performed
R2196	T3485	T3484	punct	-,PCR
R2197	T3486	T3471	auxpass	was,performed
R2198	T3487	T3471	prep	on,performed
R2199	T3488	T3487	pobj	cDNAs,on
R2200	T3489	T3488	prep	of,cDNAs
R2201	T3490	T3491	amod	pooled,liver
R2202	T3491	T3489	pobj	liver,of
R2203	T3492	T3491	compound	mouse,liver
R2204	T3493	T3471	punct	.,performed
R2205	T3495	T3496	advmod	Briefly,transcribed
R2206	T3497	T3496	punct	", ",transcribed
R2207	T3498	T3496	nsubjpass	samples,transcribed
R2208	T3499	T3498	prep	of,samples
R2209	T3500	T3501	amod	total,RNA
R2210	T3501	T3499	pobj	RNA,of
R2211	T3502	T3503	punct	(,μg
R2212	T3503	T3501	parataxis	μg,RNA
R2213	T3504	T3503	nummod	0.5,μg
R2214	T3505	T3503	punct	),μg
R2215	T3506	T3501	prep	from,RNA
R2216	T3507	T3508	amod	pooled,livers
R2217	T3508	T3506	pobj	livers,from
R2218	T3509	T3508	compound	mouse,livers
R2219	T3510	T3511	punct	(,4
R2220	T3511	T3498	parataxis	4,samples
R2221	T3512	T3511	nsubj	n,4
R2222	T3513	T3511	punct	=,4
R2223	T3514	T3511	punct	),4
R2224	T3515	T3496	auxpass	were,transcribed
R2225	T3516	T3496	advmod	reverse,transcribed
R2226	T3517	T3496	prep	according,transcribed
R2227	T3518	T3517	prep	to,according
R2228	T3519	T3520	det	the,System
R2230	T3521	T3520	nmod	SuperScript™,System
R2231	T3522	T3523	amod	First,Strand
R2232	T3523	T3525	compound	Strand,Synthesis
R2233	T3524	T3523	punct	-,Strand
R2234	T3525	T3520	compound	Synthesis,System
R2235	T3526	T3527	punct	(,Invitrogen
R2236	T3527	T3520	parataxis	Invitrogen,System
R2237	T3528	T3527	punct	", ",Invitrogen
R2238	T3529	T3527	npadvmod	Carlsbad,Invitrogen
R2239	T3530	T3527	punct	", ",Invitrogen
R2240	T3531	T3527	npadvmod	CA,Invitrogen
R2241	T3532	T3527	punct	", ",Invitrogen
R2242	T3533	T3527	npadvmod	USA,Invitrogen
R2243	T3534	T3527	punct	),Invitrogen
R2244	T3535	T3496	advcl	using,transcribed
R2245	T3536	T3537	amod	random,hexamers
R2246	T3537	T3535	dobj	hexamers,using
R2247	T3538	T3535	prep	in,using
R2248	T3539	T3540	det	a,volume
R2249	T3540	T3538	pobj	volume,in
R2250	T3541	T3540	amod	final,volume
R2251	T3542	T3543	amod	total,reaction
R2252	T3543	T3540	compound	reaction,volume
R2253	T3544	T3540	prep	of,volume
R2254	T3545	T3546	nummod	20,μl
R2255	T3546	T3544	pobj	μl,of
R2256	T3547	T3496	punct	.,transcribed
R2257	T3549	T3550	compound	RT,PCR
R2258	T3550	T3552	nsubjpass	PCR,performed
R2259	T3551	T3550	punct	-,PCR
R2260	T3553	T3552	auxpass	was,performed
R2261	T3554	T3552	advcl	using,performed
R2262	T3555	T3554	dobj	primers,using
R2263	T3556	T3555	acl	located,primers
R2264	T3557	T3556	prep	outside,located
R2265	T3558	T3557	prep	of,outside
R2266	T3559	T3560	det	the,region
R2267	T3560	T3558	pobj	region,of
R2268	T3561	T3560	amod	targeted,region
R2269	T3562	T3555	punct	: ,primers
R2270	T3563	T3555	appos	F1,primers
R2271	T3564	T3563	punct	(,F1
R2272	T3565	T3563	appos	CCTCAGCTGGTGAGGAGGTG,F1
R2273	T3566	T3563	punct	),F1
R2274	T3567	T3563	prep	with,F1
R2275	T3568	T3567	pobj	R1,with
R2276	T3569	T3568	punct	(,R1
R2277	T3570	T3568	appos	GATGGAGAAGGTGAAGTTGCC,R1
R2278	T3571	T3563	punct	),F1
R2279	T3572	T3563	punct	", ",F1
R2280	T3573	T3563	conj	F2,F1
R2281	T3574	T3573	punct	(,F2
R2282	T3575	T3573	appos	ATTTCCAATGACTTCCGGGAC,F2
R2283	T3576	T3573	punct	),F2
R2284	T3577	T3573	prep	with,F2
R2285	T3578	T3577	pobj	R1,with
R2286	T3579	T3573	cc	and,F2
R2287	T3580	T3573	conj	F3,F2
R2288	T3581	T3580	punct	(,F3
R2289	T3582	T3580	appos	CTGGAAGACGGGCTGTACACT,F3
R2290	T3583	T3580	punct	),F3
R2291	T3584	T3580	prep	with,F3
R2292	T3585	T3584	pobj	R1,with
R2293	T3586	T3554	punct	", ",using
R2294	T3587	T3588	aux	to,produce
R2295	T3588	T3554	advcl	produce,using
R2296	T3589	T3590	amod	expected,lengths
R2297	T3590	T3588	dobj	lengths,produce
R2298	T3591	T3590	compound	product,lengths
R2299	T3592	T3590	prep	of,lengths
R2300	T3593	T3594	nummod	1449,bp
R2301	T3594	T3592	pobj	bp,of
R2302	T3595	T3593	punct	", ",1449
R2303	T3596	T3593	conj	654,1449
R2304	T3597	T3596	cc	and,654
R2305	T3598	T3596	conj	429,654
R2306	T3599	T3594	advmod	respectively,bp
R2307	T3600	T3588	prep	in,produce
R2308	T3601	T3602	amod	wild,type
R2309	T3602	T3604	compound	type,cDNA
R2310	T3603	T3602	punct	-,type
R2311	T3604	T3600	pobj	cDNA,in
R2312	T3605	T3552	punct	.,performed
R2315	T3689	T3690	amod	Quantitative,PCR
R2316	T3691	T3690	compound	RT,PCR
R2317	T3692	T3690	punct	-,PCR
R2318	T3694	T3695	nsubjpass	Primers,based
R2319	T3696	T3695	auxpass	were,based
R2320	T3697	T3695	prep	upon,based
R2321	T3698	T3699	advmod	previously,published
R2322	T3699	T3700	amod	published,sets
R2323	T3700	T3697	pobj	sets,upon
R2324	T3701	T3700	compound	primer,sets
R2325	T3702	T3703	punct	[,24
R2326	T3703	T3695	parataxis	24,based
R2327	T3704	T3705	punct	-,26
R2328	T3705	T3703	prep	26,24
R2329	T3706	T3703	punct	],24
R2330	T3707	T3695	cc	or,based
R2331	T3708	T3695	conj	designed,based
R2332	T3709	T3708	advcl	using,designed
R2333	T3710	T3709	dobj	MacVector,using
R2334	T3711	T3708	punct	", ",designed
R2335	T3712	T3713	dep	which,designed
R2336	T3713	T3708	conj	designed,designed
R2337	T3714	T3713	auxpass	were,designed
R2338	T3715	T3713	prep	from,designed
R2339	T3716	T3717	preconj	either,mRNA
R2340	T3717	T3715	pobj	mRNA,from
R2341	T3718	T3717	cc	or,mRNA
R2342	T3719	T3717	conj	cDNA,mRNA
R2343	T3720	T3721	aux	to,avoid
R2344	T3721	T3713	advcl	avoid,designed
R2345	T3722	T3723	det	the,amplification
R2346	T3723	T3721	dobj	amplification,avoid
R2347	T3724	T3723	prep	of,amplification
R2348	T3725	T3726	advmod	potentially,contaminating
R2349	T3726	T3727	amod	contaminating,DNA
R2350	T3727	T3724	pobj	DNA,of
R2351	T3728	T3727	amod	genomic,DNA
R2352	T3729	T3723	prep	in,amplification
R2353	T3730	T3731	det	the,sample
R2354	T3731	T3729	pobj	sample,in
R2355	T3732	T3731	amod	total,sample
R2356	T3733	T3731	compound	RNA,sample
R2357	T3734	T3735	punct	(,Table
R2358	T3735	T3713	parataxis	Table,designed
R2359	T3736	T3735	nummod	1,Table
R2360	T3737	T3735	punct	),Table
R2361	T3738	T3695	punct	.,based
R2362	T3740	T3741	nsubjpass	Samples,transcribed
R2363	T3742	T3740	prep	of,Samples
R2364	T3743	T3744	amod	total,RNA
R2365	T3744	T3742	pobj	RNA,of
R2366	T3745	T3746	punct	(,μg
R2367	T3746	T3744	parataxis	μg,RNA
R2368	T3747	T3746	nummod	0.5,μg
R2369	T3748	T3746	punct	),μg
R2370	T3749	T3744	prep	from,RNA
R2371	T3750	T3751	amod	pooled,livers
R2372	T3751	T3749	pobj	livers,from
R2373	T3752	T3751	compound	mouse,livers
R2374	T3753	T3754	punct	(,4
R2375	T3754	T3740	parataxis	4,Samples
R2376	T3755	T3754	nsubj	n,4
R2377	T3756	T3754	punct	=,4
R2378	T3757	T3754	punct	),4
R2379	T3758	T3741	auxpass	were,transcribed
R2380	T3759	T3741	advmod	reverse,transcribed
R2381	T3760	T3741	prep	according,transcribed
R2382	T3761	T3760	prep	to,according
R2383	T3762	T3763	det	the,System
R2384	T3763	T3761	pobj	System,to
R2385	T3764	T3763	nmod	SuperScript™,System
R2386	T3765	T3766	amod	First,Strand
R2387	T3766	T3768	compound	Strand,Synthesis
R2388	T3767	T3766	punct	-,Strand
R2389	T3768	T3763	compound	Synthesis,System
R2390	T3769	T3770	punct	(,Invitrogen
R2391	T3770	T3763	parataxis	Invitrogen,System
R2392	T3771	T3770	punct	", ",Invitrogen
R2393	T3772	T3770	npadvmod	Carlsbad,Invitrogen
R2394	T3773	T3770	punct	", ",Invitrogen
R2395	T3774	T3770	npadvmod	CA,Invitrogen
R2396	T3775	T3770	punct	", ",Invitrogen
R2397	T3776	T3770	npadvmod	USA,Invitrogen
R2398	T3777	T3770	punct	),Invitrogen
R2399	T3778	T3741	advcl	using,transcribed
R2400	T3779	T3780	amod	random,hexamers
R2401	T3780	T3778	dobj	hexamers,using
R2402	T3781	T3780	prep	with,hexamers
R2403	T3782	T3783	det	a,volume
R2404	T3783	T3781	pobj	volume,with
R2405	T3784	T3783	amod	final,volume
R2406	T3785	T3786	amod	total,reaction
R2407	T3786	T3783	compound	reaction,volume
R2408	T3787	T3783	prep	of,volume
R2409	T3788	T3789	nummod	20,μl
R2410	T3789	T3787	pobj	μl,of
R2411	T3790	T3741	punct	.,transcribed
R2412	T3792	T3793	amod	Quantitative,PCR
R2413	T3793	T3796	nsubjpass	PCR,performed
R2414	T3794	T3793	compound	RT,PCR
R2415	T3795	T3793	punct	-,PCR
R2416	T3797	T3796	auxpass	was,performed
R2417	T3798	T3796	prep	on,performed
R2418	T3799	T3800	det	a,System
R2419	T3800	T3798	pobj	System,on
R2420	T3801	T3802	nmod	PE,Biosystems
R2421	T3802	T3800	nmod	Biosystems,System
R2422	T3803	T3800	nmod	GeneAmp®,System
R2423	T3804	T3800	nummod	5700,System
R2424	T3805	T3806	compound	sequence,Detection
R2425	T3806	T3800	compound	Detection,System
R2426	T3807	T3808	punct	(,City
R2427	T3808	T3800	parataxis	City,System
R2428	T3809	T3808	compound	Forest,City
R2429	T3810	T3808	punct	", ",City
R2430	T3811	T3808	npadvmod	CA,City
R2431	T3812	T3808	punct	", ",City
R2432	T3813	T3808	npadvmod	USA,City
R2433	T3814	T3808	punct	),City
R2434	T3815	T3796	punct	.,performed
R2435	T3817	T3818	amod	Standard,volume
R2436	T3818	T3820	nsubj	volume,was
R2437	T3819	T3818	compound	reaction,volume
R2438	T3821	T3822	nummod	10,μl
R2439	T3822	T3820	attr	μl,was
R2440	T3823	T3822	acl	containing,μl
R2441	T3824	T3825	nummod	1,mix
R2442	T3825	T3823	dobj	mix,containing
R2443	T3826	T3824	punct	×,1
R2444	T3827	T3825	compound	QuantiTect,mix
R2445	T3828	T3825	compound	SYBR,mix
R2446	T3829	T3825	compound	Green,mix
R2447	T3830	T3825	compound	PCR,mix
R2448	T3831	T3825	compound	master,mix
R2449	T3832	T3833	punct	(,Qiagen
R2450	T3833	T3822	parataxis	Qiagen,μl
R2451	T3834	T3833	punct	", ",Qiagen
R2452	T3835	T3833	npadvmod	Valencia,Qiagen
R2453	T3836	T3833	punct	", ",Qiagen
R2454	T3837	T3833	npadvmod	CA,Qiagen
R2455	T3838	T3833	punct	", ",Qiagen
R2456	T3839	T3833	npadvmod	USA,Qiagen
R2457	T3840	T3833	punct	),Qiagen
R2458	T3841	T3822	punct	", ",μl
R2459	T3842	T3843	nummod	0.002,U
R2460	T3843	T3844	compound	U,enzyme
R2461	T3844	T3822	conj	enzyme,μl
R2462	T3845	T3844	compound	AmpErase,enzyme
R2463	T3846	T3844	compound	UNG,enzyme
R2464	T3847	T3848	punct	(,Biosystems
R2465	T3848	T3844	parataxis	Biosystems,enzyme
R2466	T3849	T3848	compound	PE,Biosystems
R2467	T3850	T3848	punct	", ",Biosystems
R2468	T3851	T3852	compound	Forest,City
R2469	T3852	T3848	npadvmod	City,Biosystems
R2470	T3853	T3848	punct	", ",Biosystems
R2471	T3854	T3848	npadvmod	CA,Biosystems
R2472	T3855	T3848	punct	", ",Biosystems
R2473	T3856	T3848	npadvmod	USA,Biosystems
R2474	T3857	T3848	punct	),Biosystems
R2475	T3858	T3844	punct	", ",enzyme
R2476	T3859	T3860	nummod	0.7,μl
R2477	T3860	T3844	conj	μl,enzyme
R2478	T3861	T3860	prep	of,μl
R2479	T3862	T3863	compound	cDNA,template
R2480	T3863	T3861	pobj	template,of
R2481	T3864	T3860	prep	from,μl
R2482	T3865	T3866	amod	reverse,transcription
R2483	T3866	T3867	compound	transcription,reaction
R2484	T3867	T3864	pobj	reaction,from
R2485	T3868	T3860	prep	as,μl
R2486	T3869	T3868	pcomp	above,as
R2487	T3870	T3860	cc	and,μl
R2488	T3871	T3872	quantmod	100,500
R2489	T3872	T3874	nummod	500,nM
R2490	T3873	T3872	punct	–,500
R2491	T3874	T3860	conj	nM,μl
R2492	T3875	T3874	prep	of,nM
R2493	T3876	T3877	compound	oligonucleotide,primers
R2494	T3877	T3875	pobj	primers,of
R2495	T3878	T3820	punct	.,was
R2496	T3880	T3881	amod	Initial,steps
R2497	T3881	T3882	nsubj	steps,were
R2498	T3883	T3881	prep	of,steps
R2499	T3884	T3885	compound	RT,PCR
R2500	T3885	T3883	pobj	PCR,of
R2501	T3886	T3885	punct	-,PCR
R2502	T3887	T3888	nummod	two,minutes
R2503	T3888	T3882	attr	minutes,were
R2504	T3889	T3888	prep	at,minutes
R2505	T3890	T3891	nummod	50,°C
R2506	T3891	T3889	pobj	°C,at
R2507	T3892	T3888	prep	for,minutes
R2508	T3893	T3894	compound	UNG,activation
R2509	T3894	T3892	pobj	activation,for
R2510	T3895	T3888	punct	", ",minutes
R2511	T3896	T3888	acl	followed,minutes
R2512	T3897	T3896	agent	by,followed
R2513	T3898	T3899	det	a,hold
R2514	T3899	T3897	pobj	hold,by
R2515	T3900	T3901	nummod	15,minute
R2516	T3901	T3899	compound	minute,hold
R2517	T3902	T3899	prep	at,hold
R2518	T3903	T3904	nummod	95,°C
R2519	T3904	T3902	pobj	°C,at
R2520	T3905	T3882	punct	.,were
R2521	T3907	T3908	nsubj	Cycles,consisted
R2522	T3909	T3910	punct	(,40
R2523	T3910	T3907	parataxis	40,Cycles
R2524	T3911	T3910	nsubj	n,40
R2525	T3912	T3910	punct	=,40
R2526	T3913	T3910	punct	),40
R2527	T3914	T3908	prep	of,consisted
R2528	T3915	T3916	det	a,melt
R2529	T3916	T3914	pobj	melt,of
R2530	T3917	T3918	nummod	15,second
R2531	T3918	T3916	nmod	second,melt
R2532	T3919	T3916	prep	at,melt
R2533	T3920	T3921	nummod	95,°C
R2534	T3921	T3919	pobj	°C,at
R2535	T3922	T3916	punct	", ",melt
R2536	T3923	T3916	acl	followed,melt
R2537	T3924	T3923	agent	by,followed
R2538	T3925	T3926	det	a,minute
R2539	T3926	T3924	pobj	minute,by
R2540	T3927	T3926	nummod	1,minute
R2541	T3928	T3929	compound	annealing,extension
R2542	T3929	T3926	appos	extension,minute
R2543	T3930	T3929	punct	/,extension
R2544	T3931	T3926	prep	at,minute
R2545	T3932	T3933	nummod	60,°C
R2546	T3933	T3931	pobj	°C,at
R2547	T3934	T3908	punct	.,consisted
R2548	T3936	T3937	det	The,step
R2549	T3937	T3939	nsubj	step,was
R2550	T3938	T3937	amod	final,step
R2551	T3940	T3941	det	a,incubation
R2552	T3941	T3939	attr	incubation,was
R2553	T3942	T3943	nummod	60,°C
R2554	T3943	T3941	compound	°C,incubation
R2555	T3944	T3941	prep	for,incubation
R2556	T3945	T3946	nummod	1,minute
R2557	T3946	T3944	pobj	minute,for
R2558	T3947	T3939	punct	.,was
R2559	T3949	T3950	prep	At,heated
R2560	T3951	T3952	det	the,end
R2561	T3952	T3949	pobj	end,At
R2562	T3953	T3952	prep	of,end
R2563	T3954	T3955	det	the,run
R2564	T3955	T3953	pobj	run,of
R2565	T3956	T3950	punct	", ",heated
R2566	T3957	T3950	nsubjpass	samples,heated
R2567	T3958	T3950	auxpass	were,heated
R2568	T3959	T3950	prep	to,heated
R2569	T3960	T3961	nummod	95,°C
R2570	T3961	T3959	pobj	°C,to
R2571	T3962	T3950	prep	with,heated
R2572	T3963	T3964	det	a,time
R2573	T3964	T3962	pobj	time,with
R2574	T3965	T3964	compound	ramp,time
R2575	T3966	T3964	prep	of,time
R2576	T3967	T3968	nummod	20,minutes
R2577	T3968	T3966	pobj	minutes,of
R2578	T3969	T3970	aux	to,construct
R2579	T3970	T3950	advcl	construct,heated
R2580	T3971	T3972	compound	dissociation,curves
R2581	T3972	T3970	dobj	curves,construct
R2582	T3973	T3974	aux	to,ensure
R2583	T3974	T3970	advcl	ensure,construct
R2584	T3975	T3976	mark	that,obtained
R2585	T3976	T3974	ccomp	obtained,ensure
R2586	T3977	T3978	amod	single,products
R2587	T3978	T3976	nsubjpass	products,obtained
R2588	T3979	T3978	compound	PCR,products
R2589	T3980	T3976	auxpass	were,obtained
R2590	T3981	T3950	punct	.,heated
R2591	T3983	T3984	det	All,reactions
R2592	T3984	T3985	nsubjpass	reactions,performed
R2593	T3986	T3985	auxpass	were,performed
R2594	T3987	T3985	prep	in,performed
R2595	T3988	T3987	pobj	triplicate,in
R2596	T3989	T3985	punct	.,performed
R2597	T3991	T3992	nmod	Threshold,cycle
R2598	T3992	T3993	nmod	cycle,analysis
R2599	T3993	T3997	nsubjpass	analysis,set
R2600	T3994	T3992	punct	(,cycle
R2601	T3995	T3992	appos	CT,cycle
R2602	T3996	T3993	punct	),analysis
R2603	T3998	T3993	prep	of,analysis
R2604	T3999	T4000	det	all,samples
R2605	T4000	T3998	pobj	samples,of
R2606	T4001	T3997	auxpass	was,set
R2607	T4002	T3997	prep	at,set
R2608	T4003	T4004	nummod	0.5,units
R2609	T4004	T4002	pobj	units,at
R2610	T4005	T4006	amod	relative,fluorescence
R2611	T4006	T4004	compound	fluorescence,units
R2612	T4007	T3997	punct	.,set
R2613	T4009	T4010	det	The,quantities
R2614	T4010	T4012	nsubjpass	quantities,normalized
R2615	T4011	T4010	amod	relative,quantities
R2616	T4013	T4010	prep	of,quantities
R2617	T4014	T4013	pobj	message,of
R2618	T4015	T4010	prep	of,quantities
R2619	T4016	T4015	pobj	genes,of
R2620	T4017	T4016	prep	of,genes
R2621	T4018	T4017	pobj	interest,of
R2622	T4019	T4010	prep	from,quantities
R2623	T4020	T4021	det	the,samples
R2624	T4021	T4019	pobj	samples,from
R2625	T4022	T4023	compound	mouse,liver
R2626	T4023	T4021	compound	liver,samples
R2627	T4024	T4021	acl	used,samples
R2628	T4025	T4024	prep	in,used
R2629	T4026	T4027	amod	real,time
R2630	T4027	T4029	compound	time,PCR
R2631	T4028	T4027	punct	-,time
R2632	T4029	T4025	pobj	PCR,in
R2633	T4030	T4029	compound	RT,PCR
R2634	T4031	T4029	punct	-,PCR
R2635	T4032	T4012	auxpass	were,normalized
R2636	T4033	T4012	prep	to,normalized
R2637	T4034	T4033	pobj	cyclophilin,to
R2638	T4035	T4036	aux	to,compensate
R2639	T4036	T4012	advcl	compensate,normalized
R2640	T4037	T4036	prep	for,compensate
R2641	T4038	T4037	pobj	variations,for
R2642	T4039	T4038	prep	in,variations
R2643	T4040	T4041	compound	input,RNA
R2644	T4041	T4042	compound	RNA,amounts
R2645	T4042	T4039	pobj	amounts,in
R2646	T4043	T4012	punct	.,normalized
R2647	T4045	T4046	det	The,data
R2648	T4046	T4047	nsubjpass	data,analyzed
R2649	T4048	T4047	auxpass	were,analyzed
R2650	T4049	T4047	advcl	using,analyzed
R2651	T4050	T4051	det	the,method
R2652	T4051	T4049	dobj	method,using
R2653	T4052	T4051	amod	comparative,method
R2654	T4053	T4054	compound	threshold,cycle
R2655	T4054	T4051	compound	cycle,method
R2656	T4055	T4051	punct	(,method
R2657	T4056	T4051	appos	CT,method
R2658	T4057	T4047	punct	),analyzed
R2659	T4058	T4047	punct	.,analyzed
R2660	T4060	T4061	advmod	Briefly,averaged
R2661	T4062	T4061	punct	", ",averaged
R2662	T4063	T4064	det	the,values
R2663	T4064	T4061	nsubjpass	values,averaged
R2664	T4065	T4064	compound	CT,values
R2665	T4066	T4061	auxpass	are,averaged
R2666	T4067	T4061	punct	", ",averaged
R2667	T4068	T4061	prep	from,averaged
R2668	T4069	T4070	det	the,triplicates
R2669	T4070	T4068	pobj	triplicates,from
R2670	T4071	T4070	acl	defining,triplicates
R2671	T4072	T4073	det	a,value
R2672	T4073	T4071	dobj	value,defining
R2673	T4074	T4075	compound	Δ,CT
R2674	T4075	T4073	compound	CT,value
R2675	T4076	T4075	punct	-,CT
R2676	T4077	T4073	acl	calculated,value
R2677	T4078	T4077	prep	by,calculated
R2678	T4079	T4078	pcomp	taking,by
R2679	T4080	T4081	det	the,CT
R2680	T4081	T4079	dobj	CT,taking
R2681	T4082	T4081	amod	average,CT
R2682	T4083	T4081	prep	of,CT
R2683	T4084	T4085	det	the,gene
R2684	T4085	T4083	pobj	gene,of
R2685	T4086	T4085	prep	of,gene
R2686	T4087	T4086	pobj	interest,of
R2687	T4088	T4079	cc	and,taking
R2688	T4089	T4079	conj	subtracting,taking
R2689	T4090	T4089	dobj	it,subtracting
R2690	T4091	T4089	prep	from,subtracting
R2691	T4092	T4093	det	the,CT
R2692	T4093	T4091	pobj	CT,from
R2693	T4094	T4093	amod	average,CT
R2694	T4095	T4093	prep	of,CT
R2695	T4096	T4095	pobj	cyclophilin,of
R2696	T4097	T4061	punct	.,averaged
R2697	T4099	T4100	det	The,CT
R2698	T4100	T4103	nsubjpass	CT,calculated
R2699	T4101	T4100	compound	ΔΔ,CT
R2700	T4102	T4100	punct	-,CT
R2701	T4104	T4103	auxpass	was,calculated
R2702	T4105	T4103	prep	by,calculated
R2703	T4106	T4105	pcomp	subtracting,by
R2704	T4107	T4108	det	the,values
R2706	T4109	T4108	amod	average,values
R2707	T4110	T4111	nmod	Δ,CT
R2708	T4111	T4108	nmod	CT,values
R2709	T4112	T4111	punct	-,CT
R2710	T4113	T4114	punct	(,calibrator
R2711	T4114	T4111	parataxis	calibrator,CT
R2712	T4115	T4114	punct	),calibrator
R2713	T4116	T4106	prep	from,subtracting
R2714	T4117	T4118	det	the,CT
R2715	T4118	T4116	pobj	CT,from
R2716	T4119	T4118	compound	Δ,CT
R2717	T4120	T4118	punct	-,CT
R2718	T4121	T4122	punct	(,sample
R2719	T4122	T4118	parataxis	sample,CT
R2720	T4123	T4122	punct	),sample
R2721	T4124	T4103	punct	.,calculated
R2722	T4126	T4127	det	The,quantification
R2723	T4127	T4129	nsubjpass	quantification,calculated
R2724	T4128	T4127	amod	relative,quantification
R2725	T4130	T4129	auxpass	was,calculated
R2726	T4131	T4129	advmod	then,calculated
R2727	T4132	T4129	agent	by,calculated
R2728	T4133	T4134	det	the,CT
R2729	T4134	T4132	pobj	CT,by
R2730	T4135	T4134	nmod	expression,CT
R2731	T4136	T4134	nummod	2,CT
R2732	T4137	T4134	punct	-,CT
R2733	T4138	T4134	nmod	AverageΔΔ,CT
R2734	T4139	T4134	punct	-,CT
R2735	T4140	T4129	punct	.,calculated
R2736	T4142	T4143	det	The,quantity
R2737	T4143	T4145	nsubjpass	quantity,expressed
R2738	T4144	T4143	compound	mRNA,quantity
R2739	T4146	T4143	prep	for,quantity
R2740	T4147	T4148	det	the,calibrator
R2741	T4148	T4146	pobj	calibrator,for
R2742	T4149	T4150	punct	(,type
R2743	T4150	T4148	parataxis	type,calibrator
R2744	T4151	T4150	amod	wild,type
R2745	T4152	T4150	punct	),type
R2746	T4153	T4145	auxpass	was,expressed
R2747	T4154	T4145	prep	as,expressed
R2748	T4155	T4154	pobj	1,as
R2749	T4156	T4145	cc	and,expressed
R2750	T4157	T4158	det	all,quantities
R2751	T4158	T4160	nsubjpass	quantities,expressed
R2752	T4159	T4158	amod	other,quantities
R2753	T4160	T4145	conj	expressed,expressed
R2754	T4161	T4160	auxpass	were,expressed
R2755	T4162	T4160	prep	as,expressed
R2756	T4163	T4164	det	a,difference
R2757	T4164	T4162	pobj	difference,as
R2758	T4165	T4164	compound	fold,difference
R2759	T4166	T4164	amod	relative,difference
R2760	T4167	T4166	prep	to,relative
R2761	T4168	T4169	det	the,calibrator
R2762	T4169	T4167	pobj	calibrator,to
R2763	T4170	T4171	punct	(,type
R2764	T4171	T4169	parataxis	type,calibrator
R2765	T4172	T4171	amod	wild,type
R2766	T4173	T4171	punct	),type
R2767	T4174	T4160	punct	.,expressed
R2768	T4217	T4218	compound	Plasma,lipid
R2769	T4218	T4219	compound	lipid,analysis
R2770	T4220	T4219	prep	by,analysis
R2771	T4221	T4222	amod	fast,protein
R2772	T4222	T4223	compound	protein,chromatography
R2773	T4223	T4220	pobj	chromatography,by
R2774	T4224	T4223	compound	liquid,chromatography
R2775	T4226	T4227	prep	Following,collected
R2776	T4228	T4229	nummod	16,hrs
R2777	T4229	T4226	pobj	hrs,Following
R2778	T4230	T4229	prep	of,hrs
R2779	T4231	T4230	pobj	fasting,of
R2780	T4232	T4227	punct	", ",collected
R2781	T4233	T4234	compound	blood,samples
R2782	T4234	T4227	nsubjpass	samples,collected
R2783	T4235	T4236	punct	(,3
R2784	T4236	T4234	parataxis	3,samples
R2785	T4237	T4236	nsubj	n,3
R2786	T4238	T4236	punct	=,3
R2787	T4239	T4236	punct	),3
R2788	T4240	T4234	prep	from,samples
R2789	T4241	T4240	pobj	each,from
R2790	T4242	T4241	prep	of,each
R2791	T4243	T4244	det	the,mice
R2792	T4244	T4242	pobj	mice,of
R2793	T4245	T4244	nmod	Abcg8,mice
R2794	T4246	T4245	punct	+,Abcg8
R2795	T4247	T4245	punct	/,Abcg8
R2796	T4248	T4245	punct	+,Abcg8
R2797	T4249	T4245	punct	", ",Abcg8
R2798	T4250	T4245	conj	Abcg8,Abcg8
R2799	T4251	T4250	punct	+,Abcg8
R2800	T4252	T4250	punct	/,Abcg8
R2801	T4253	T4250	punct	-,Abcg8
R2802	T4254	T4250	cc	and,Abcg8
R2803	T4255	T4250	conj	Abcg8,Abcg8
R2804	T4256	T4255	punct	-,Abcg8
R2805	T4257	T4255	punct	/,Abcg8
R2806	T4258	T4255	punct	-,Abcg8
R2807	T4259	T4227	auxpass	were,collected
R2808	T4260	T4227	prep	from,collected
R2809	T4261	T4262	det	the,plexus
R2810	T4262	T4260	pobj	plexus,from
R2811	T4263	T4264	amod	retro,orbital
R2812	T4264	T4262	amod	orbital,plexus
R2813	T4265	T4264	punct	-,orbital
R2814	T4266	T4262	compound	venous,plexus
R2815	T4267	T4227	advcl	using,collected
R2816	T4268	T4269	amod	heparinized,tubes
R2817	T4269	T4267	dobj	tubes,using
R2818	T4270	T4269	amod	capillary,tubes
R2819	T4271	T4227	prep	under,collected
R2820	T4272	T4273	compound	isoflurane,anesthesia
R2821	T4273	T4271	pobj	anesthesia,under
R2822	T4274	T4227	punct	", ",collected
R2823	T4275	T4227	cc	and,collected
R2824	T4276	T4227	conj	placed,collected
R2825	T4277	T4276	prep	into,placed
R2826	T4278	T4279	amod	precooled,tubes
R2827	T4279	T4277	pobj	tubes,into
R2828	T4280	T4279	acl	containing,tubes
R2829	T4281	T4282	nummod	10,μl
R2830	T4282	T4280	dobj	μl,containing
R2831	T4283	T4282	prep	of,μl
R2832	T4284	T4285	nummod	0.5,M
R2833	T4285	T4286	compound	M,EDTA
R2834	T4286	T4283	pobj	EDTA,of
R2835	T4287	T4227	punct	.,collected
R2836	T4289	T4290	amod	Equal,volumes
R2837	T4290	T4291	nsubjpass	volumes,pooled
R2838	T4292	T4290	prep	of,volumes
R2839	T4293	T4292	pobj	plasma,of
R2840	T4294	T4293	prep	from,plasma
R2841	T4295	T4296	det	each,genotype
R2842	T4296	T4294	pobj	genotype,from
R2843	T4297	T4291	auxpass	were,pooled
R2844	T4298	T4291	cc	and,pooled
R2845	T4299	T4300	nummod	100,μL
R2846	T4300	T4301	nsubjpass	μL,injected
R2847	T4301	T4291	conj	injected,pooled
R2848	T4302	T4300	prep	of,μL
R2849	T4303	T4304	det	the,sample
R2850	T4304	T4302	pobj	sample,of
R2851	T4305	T4304	amod	pooled,sample
R2852	T4306	T4301	auxpass	were,injected
R2853	T4307	T4301	cc	and,injected
R2854	T4308	T4301	conj	fractionated,injected
R2855	T4309	T4308	prep	by,fractionated
R2856	T4310	T4311	amod	fast,protein
R2857	T4311	T4312	compound	protein,chromatography
R2858	T4312	T4309	pobj	chromatography,by
R2859	T4313	T4312	compound	liquid,chromatography
R2860	T4314	T4312	punct	(,chromatography
R2861	T4315	T4312	appos	FPLC,chromatography
R2862	T4316	T4308	punct	),fractionated
R2863	T4317	T4308	advcl	using,fractionated
R2864	T4318	T4319	det	a,column
R2865	T4319	T4317	dobj	column,using
R2866	T4320	T4319	nmod	Superose,column
R2867	T4321	T4320	nummod	6,Superose
R2868	T4322	T4319	nmod	HR10,column
R2869	T4323	T4322	punct	/,HR10
R2870	T4324	T4322	nummod	30,HR10
R2871	T4325	T4326	punct	(,Inc.
R2872	T4326	T4319	parataxis	Inc.,column
R2873	T4327	T4326	compound	Pharmacia,Inc.
R2874	T4328	T4326	compound	Biotech,Inc.
R2875	T4329	T4326	punct	", ",Inc.
R2876	T4330	T4326	npadvmod	Piscataway,Inc.
R2877	T4331	T4326	punct	", ",Inc.
R2878	T4332	T4326	npadvmod	NJ,Inc.
R2879	T4333	T4326	punct	", ",Inc.
R2880	T4334	T4326	npadvmod	USA,Inc.
R2881	T4335	T4326	punct	),Inc.
R2882	T4336	T4301	cc	and,injected
R2883	T4337	T4301	conj	analyzed,injected
R2884	T4338	T4339	mark	as,described
R2885	T4339	T4337	advcl	described,analyzed
R2886	T4340	T4341	punct	[,27
R2887	T4341	T4301	parataxis	27,injected
R2888	T4342	T4341	punct	],27
R2889	T4343	T4301	punct	.,injected
R2890	T4462	T4463	compound	Sterol,composition
R2891	T4464	T4463	prep	of,composition
R2892	T4465	T4464	pobj	plasma,of
R2893	T4466	T4465	cc	and,plasma
R2894	T4467	T4465	conj	tissues,plasma
R2895	T4469	T4470	prep	Following,collected
R2896	T4471	T4472	det	a,fast
R2897	T4472	T4469	pobj	fast,Following
R2898	T4473	T4474	nummod	four,hour
R2899	T4474	T4472	compound	hour,fast
R2900	T4475	T4474	punct	-,hour
R2901	T4476	T4472	prep	of,fast
R2902	T4477	T4478	nummod	12,week
R2903	T4478	T4480	npadvmod	week,old
R2904	T4479	T4478	punct	-,week
R2905	T4480	T4482	amod	old,mice
R2906	T4481	T4480	punct	-,old
R2907	T4482	T4476	pobj	mice,of
R2908	T4483	T4482	acl	fed,mice
R2909	T4484	T4483	prep	on,fed
R2910	T4485	T4486	amod	regular,diet
R2911	T4486	T4484	pobj	diet,on
R2912	T4487	T4488	compound	rodent,chow
R2913	T4488	T4486	compound	chow,diet
R2914	T4489	T4470	punct	;,collected
R2915	T4490	T4470	nsubjpass	blood,collected
R2916	T4491	T4490	cc	and,blood
R2917	T4492	T4490	conj	tissues,blood
R2918	T4493	T4470	auxpass	were,collected
R2919	T4494	T4470	prep	under,collected
R2920	T4495	T4496	compound	isofluorane,anesthesia
R2921	T4496	T4494	pobj	anesthesia,under
R2922	T4497	T4470	prep	from,collected
R2923	T4498	T4497	pobj	each,from
R2924	T4499	T4498	prep	of,each
R2925	T4500	T4501	det	the,mice
R2926	T4501	T4499	pobj	mice,of
R2927	T4502	T4501	nmod	Abcg8,mice
R2928	T4503	T4502	punct	+,Abcg8
R2929	T4504	T4502	punct	/,Abcg8
R2930	T4505	T4502	punct	+,Abcg8
R2931	T4506	T4502	punct	", ",Abcg8
R2932	T4507	T4502	conj	Abcg8,Abcg8
R2933	T4508	T4507	punct	+,Abcg8
R2934	T4509	T4507	punct	/,Abcg8
R2935	T4510	T4507	punct	-,Abcg8
R2936	T4511	T4507	cc	and,Abcg8
R2937	T4512	T4507	conj	Abcg8,Abcg8
R2938	T4513	T4512	punct	-,Abcg8
R2939	T4514	T4512	punct	/,Abcg8
R2940	T4515	T4512	punct	-,Abcg8
R2941	T4516	T4517	punct	(,3
R2942	T4517	T4501	parataxis	3,mice
R2943	T4518	T4517	nsubj	n,3
R2944	T4519	T4517	punct	=,3
R2945	T4520	T4517	prep	for,3
R2946	T4521	T4522	det	each,group
R2947	T4522	T4520	pobj	group,for
R2948	T4523	T4517	punct	),3
R2949	T4524	T4470	punct	.,collected
R2950	T4526	T4527	nsubjpass	Blood,collected
R2951	T4528	T4527	auxpass	was,collected
R2952	T4529	T4527	prep	from,collected
R2953	T4530	T4531	det	the,plexus
R2954	T4531	T4529	pobj	plexus,from
R2955	T4532	T4533	amod	retro,orbital
R2956	T4533	T4531	amod	orbital,plexus
R2957	T4534	T4533	punct	-,orbital
R2958	T4535	T4531	compound	venous,plexus
R2959	T4536	T4527	prep	by,collected
R2960	T4537	T4538	amod	heparinized,tube
R2961	T4538	T4536	pobj	tube,by
R2962	T4539	T4538	compound	glass,tube
R2963	T4540	T4527	punct	.,collected
R2964	T4542	T4543	det	The,animals
R2965	T4543	T4544	nsubjpass	animals,sacrificed
R2966	T4545	T4544	auxpass	were,sacrificed
R2967	T4546	T4544	prep	by,sacrificed
R2968	T4547	T4548	amod	cervical,dislocation
R2969	T4548	T4546	pobj	dislocation,by
R2970	T4549	T4544	prep	for,sacrificed
R2971	T4550	T4551	compound	tissue,collection
R2972	T4551	T4549	pobj	collection,for
R2973	T4552	T4544	punct	.,sacrificed
R2974	T4554	T4555	nsubjpass	Plasma,saponified
R2975	T4556	T4557	punct	(,μl
R2976	T4557	T4554	parataxis	μl,Plasma
R2977	T4558	T4557	nummod	100,μl
R2978	T4559	T4557	punct	),μl
R2979	T4560	T4555	auxpass	was,saponified
R2980	T4561	T4555	prep	in,saponified
R2981	T4562	T4563	nummod	1,N
R2982	T4563	T4564	compound	N,NaOH
R2983	T4564	T4561	pobj	NaOH,in
R2984	T4565	T4555	prep	for,saponified
R2985	T4566	T4567	nummod	one,hour
R2986	T4567	T4565	pobj	hour,for
R2987	T4568	T4555	punct	", ",saponified
R2988	T4569	T4570	nummod	1.5,mL
R2989	T4570	T4571	compound	mL,water
R2990	T4571	T4572	nsubj	water,added
R2991	T4572	T4555	conj	added,saponified
R2992	T4573	T4572	cc	and,added
R2993	T4574	T4575	nsubj	sterols,extracted
R2994	T4575	T4572	conj	extracted,added
R2995	T4576	T4575	prep	with,extracted
R2996	T4577	T4578	nummod	three,portions
R2997	T4578	T4576	pobj	portions,with
R2998	T4579	T4578	amod	sequential,portions
R2999	T4580	T4578	prep	of,portions
R3000	T4581	T4582	nummod	1.5,mL
R3001	T4582	T4583	compound	mL,acetate
R3002	T4583	T4580	pobj	acetate,of
R3003	T4584	T4583	compound	ethyl,acetate
R3004	T4585	T4583	punct	", ",acetate
R3005	T4586	T4583	acl	containing,acetate
R3006	T4587	T4588	nummod	70,μg
R3007	T4588	T4589	compound	μg,cholestane
R3008	T4589	T4586	dobj	cholestane,containing
R3009	T4590	T4589	compound	5α,cholestane
R3010	T4591	T4589	punct	-,cholestane
R3011	T4592	T4589	prep	as,cholestane
R3012	T4593	T4594	det	an,standard
R3013	T4594	T4592	pobj	standard,as
R3014	T4595	T4594	amod	internal,standard
R3015	T4596	T4575	punct	", ",extracted
R3016	T4597	T4575	conj	pooled,extracted
R3017	T4598	T4597	cc	and,pooled
R3018	T4599	T4597	conj	dried,pooled
R3019	T4600	T4575	punct	.,extracted
R3020	T4602	T4603	compound	Mouse,tissues
R3021	T4603	T4604	nsubjpass	tissues,weighed
R3022	T4605	T4603	punct	(,tissues
R3023	T4606	T4603	appos	liver,tissues
R3024	T4607	T4606	punct	", ",liver
R3025	T4608	T4606	conj	spleen,liver
R3026	T4609	T4608	cc	and,spleen
R3027	T4610	T4608	conj	brain,spleen
R3028	T4611	T4604	punct	),weighed
R3029	T4612	T4604	auxpass	were,weighed
R3030	T4613	T4604	punct	", ",weighed
R3031	T4614	T4604	dep	homogenized,weighed
R3032	T4615	T4614	prep	in,homogenized
R3033	T4616	T4617	nummod	1,mL
R3034	T4617	T4615	pobj	mL,in
R3035	T4618	T4619	compound	phosphate,buffer
R3036	T4619	T4620	compound	buffer,saline
R3037	T4620	T4617	appos	saline,mL
R3038	T4621	T4620	punct	(,saline
R3039	T4622	T4620	appos	PBS,saline
R3040	T4623	T4617	punct	),mL
R3041	T4624	T4617	prep	with,mL
R3042	T4625	T4626	det	a,homogenizer
R3043	T4626	T4624	pobj	homogenizer,with
R3044	T4627	T4626	compound	dounce,homogenizer
R3045	T4628	T4629	punct	(,strokes
R3046	T4629	T4626	parataxis	strokes,homogenizer
R3047	T4630	T4629	nummod	15,strokes
R3048	T4631	T4629	prep	at,strokes
R3049	T4632	T4633	nummod	500,rpm
R3050	T4633	T4631	pobj	rpm,at
R3051	T4634	T4629	punct	),strokes
R3052	T4635	T4626	cc	and,homogenizer
R3053	T4636	T4637	det	a,aliquot
R3054	T4637	T4626	conj	aliquot,homogenizer
R3055	T4638	T4637	amod	small,aliquot
R3056	T4639	T4637	prep	of,aliquot
R3057	T4640	T4641	det	the,homogenate
R3058	T4641	T4639	pobj	homogenate,of
R3059	T4642	T4641	amod	whole,homogenate
R3060	T4643	T4641	acl	assayed,homogenate
R3061	T4644	T4643	prep	for,assayed
R3062	T4645	T4646	compound	protein,concentration
R3063	T4646	T4644	pobj	concentration,for
R3064	T4647	T4604	punct	.,weighed
R3065	T4649	T4650	nsubjpass	Aliquots,extracted
R3066	T4651	T4649	prep	for,Aliquots
R3067	T4652	T4653	compound	sterol,analysis
R3068	T4653	T4651	pobj	analysis,for
R3069	T4654	T4649	prep	from,Aliquots
R3070	T4655	T4656	amod	whole,homogenates
R3071	T4656	T4654	pobj	homogenates,from
R3072	T4657	T4656	compound	tissue,homogenates
R3073	T4658	T4650	auxpass	were,extracted
R3074	T4659	T4660	mark	as,described
R3075	T4660	T4650	advcl	described,extracted
R3076	T4661	T4660	advmod	above,described
R3077	T4662	T4650	punct	.,extracted
R3078	T4664	T4665	amod	Dried,samples
R3079	T4665	T4667	nsubjpass	samples,derivatized
R3080	T4666	T4665	compound	sterol,samples
R3081	T4668	T4667	auxpass	were,derivatized
R3082	T4669	T4667	prep	as,derivatized
R3083	T4670	T4669	pobj	trimethylsilylethers,as
R3084	T4671	T4667	punct	", ",derivatized
R3085	T4672	T4667	dep	redissolved,derivatized
R3086	T4673	T4672	prep	in,redissolved
R3087	T4674	T4675	nummod	10,μL
R3088	T4675	T4676	compound	μL,hexane
R3089	T4676	T4673	pobj	hexane,in
R3090	T4677	T4667	cc	and,derivatized
R3091	T4678	T4679	nummod	2,μL
R3092	T4679	T4680	compound	μL,samples
R3093	T4680	T4681	nsubjpass	samples,injected
R3094	T4681	T4667	conj	injected,derivatized
R3095	T4682	T4681	auxpass	were,injected
R3096	T4683	T4681	prep	into,injected
R3097	T4684	T4685	det	a,column
R3098	T4685	T4683	pobj	column,into
R3099	T4686	T4685	amod	capillary,column
R3100	T4687	T4688	punct	(,length
R3101	T4688	T4685	parataxis	length,column
R3102	T4689	T4690	nummod	26,m
R3103	T4690	T4688	compound	m,length
R3104	T4691	T4688	punct	", ",length
R3105	T4692	T4693	nummod	0.32,mm
R3106	T4693	T4694	compound	mm,ID
R3107	T4694	T4688	appos	ID,length
R3108	T4695	T4688	punct	", ",length
R3109	T4696	T4697	nummod	0.45,mm
R3110	T4697	T4698	compound	mm,OD
R3111	T4698	T4688	appos	OD,length
R3112	T4699	T4688	punct	),length
R3113	T4700	T4685	acl	coated,column
R3114	T4701	T4700	prep	with,coated
R3115	T4702	T4703	amod	liquid,CB
R3116	T4703	T4701	pobj	CB,with
R3117	T4704	T4703	nmod	CpWAX,CB
R3118	T4705	T4703	nummod	57,CB
R3119	T4706	T4707	punct	(,Chrompak
R3120	T4707	T4703	parataxis	Chrompak,CB
R3121	T4708	T4707	punct	", ",Chrompak
R3122	T4709	T4707	npadvmod	Bridgewater,Chrompak
R3123	T4710	T4707	punct	", ",Chrompak
R3124	T4711	T4707	npadvmod	NJ,Chrompak
R3125	T4712	T4707	punct	", ",Chrompak
R3126	T4713	T4707	npadvmod	USA,Chrompak
R3127	T4714	T4707	punct	),Chrompak
R3128	T4715	T4685	prep	in,column
R3129	T4716	T4717	det	a,Chromatograph
R3130	T4717	T4715	pobj	Chromatograph,in
R3131	T4718	T4719	compound	Hewlett,Packard
R3132	T4719	T4717	compound	Packard,Chromatograph
R3133	T4720	T4719	punct	-,Packard
R3134	T4721	T4717	compound	Gas,Chromatograph
R3135	T4722	T4717	acl	equipped,Chromatograph
R3136	T4723	T4722	prep	with,equipped
R3137	T4724	T4725	det	a,detector
R3138	T4725	T4723	pobj	detector,with
R3139	T4726	T4727	compound	flame,ionization
R3140	T4727	T4725	compound	ionization,detector
R3141	T4728	T4729	punct	[,28
R3142	T4729	T4681	parataxis	28,injected
R3143	T4730	T4729	punct	],28
R3144	T4731	T4681	punct	.,injected
R3145	T4733	T4734	nsubjpass	Concentrations,reported
R3146	T4735	T4733	prep	of,Concentrations
R3147	T4736	T4737	nmod	plasma,sterols
R3148	T4737	T4735	pobj	sterols,of
R3149	T4738	T4736	cc	and,plasma
R3150	T4739	T4736	conj	tissue,plasma
R3151	T4740	T4734	auxpass	were,reported
R3152	T4741	T4734	prep	as,reported
R3153	T4742	T4743	nmod	mg,tissue
R3154	T4743	T4741	pobj	tissue,as
R3155	T4744	T4745	punct	/,dL
R3156	T4745	T4742	prep	dL,mg
R3157	T4746	T4742	cc	and,mg
R3158	T4747	T4742	conj	μg,mg
R3159	T4748	T4749	punct	/,g
R3160	T4749	T4747	prep	g,μg
R3161	T4750	T4751	amod	wet,weight
R3162	T4751	T4743	compound	weight,tissue
R3163	T4752	T4734	punct	", ",reported
R3164	T4753	T4734	advmod	respectively,reported
R3165	T4754	T4734	punct	.,reported
R3166	T4782	T4783	amod	Polyclonal,production
R3167	T4784	T4783	compound	antibody,production
R3168	T4786	T4787	nummod	Three,antibodies
R3170	T4788	T4787	amod	separate,antibodies
R3171	T4789	T4787	amod	polyclonal,antibodies
R3172	T4790	T4791	amod	anti-Abcg5,sterolin
R3173	T4791	T4787	nmod	sterolin,antibodies
R3174	T4792	T4791	punct	/,sterolin
R3175	T4793	T4791	punct	-,sterolin
R3176	T4794	T4791	nummod	1,sterolin
R3177	T4796	T4795	aux	have,raised
R3178	T4797	T4795	auxpass	been,raised
R3179	T4798	T4799	mark	as,described
R3180	T4799	T4795	advcl	described,raised
R3181	T4800	T4799	advmod	previously,described
R3182	T4801	T4802	punct	[,30
R3183	T4802	T4795	parataxis	30,raised
R3184	T4803	T4802	nummod	20,30
R3185	T4804	T4802	punct	",",30
R3186	T4805	T4802	nummod	29,30
R3187	T4806	T4802	punct	",",30
R3188	T4807	T4802	punct	],30
R3189	T4808	T4795	punct	.,raised
R3190	T4810	T4811	prep	In,labelled
R3191	T4812	T4810	pobj	experiments,In
R3192	T4813	T4811	punct	", ",labelled
R3193	T4814	T4815	amod	anti-Abcg5,sterolin
R3194	T4815	T4817	nmod	sterolin,antibodies
R3195	T4816	T4815	punct	/,sterolin
R3196	T4817	T4811	nsubjpass	antibodies,labelled
R3197	T4818	T4815	punct	-,sterolin
R3198	T4819	T4815	nummod	1,sterolin
R3199	T4820	T4811	auxpass	are,labelled
R3200	T4821	T4822	mark	as,follows
R3201	T4822	T4811	advcl	follows,labelled
R3202	T4823	T4811	punct	:,labelled
R3203	T4825	T4826	meta	1,SC
R3204	T4827	T4825	punct	),1
R3205	T4828	T4826	punct	–,SC
R3206	T4829	T4826	prep	from,SC
R3207	T4830	T4831	det	the,group
R3208	T4831	T4829	pobj	group,from
R3209	T4832	T4831	compound	Patel,group
R3210	T4833	T4826	punct	", ",SC
R3211	T4834	T4835	meta	2,AMC
R3212	T4835	T4826	conj	AMC,SC
R3213	T4836	T4834	punct	),2
R3214	T4837	T4835	punct	–,AMC
R3215	T4838	T4835	prep	from,AMC
R3216	T4839	T4840	det	the,group
R3217	T4840	T4838	pobj	group,from
R3218	T4841	T4842	compound	AMC,Center
R3219	T4842	T4840	compound	Center,group
R3220	T4843	T4842	compound	Liver,Center
R3221	T4844	T4835	punct	", ",AMC
R3222	T4845	T4835	cc	and,AMC
R3223	T4846	T4847	meta	3,UTSW
R3224	T4847	T4835	conj	UTSW,AMC
R3225	T4848	T4846	punct	),3
R3226	T4849	T4847	punct	–,UTSW
R3227	T4850	T4847	prep	from,UTSW
R3228	T4851	T4852	det	the,group
R3229	T4852	T4850	pobj	group,from
R3230	T4853	T4852	compound	Hobbs,group
R3231	T4854	T4847	punct	.,UTSW
R3232	T4905	T4906	compound	Membrane,protein
R3233	T4906	T4907	compound	protein,preparations
R3234	T4909	T4910	amod	Total,membranes
R3235	T4910	T4911	nsubjpass	membranes,prepared
R3236	T4912	T4911	auxpass	were,prepared
R3237	T4913	T4911	prep	by,prepared
R3238	T4914	T4913	pcomp	taking,by
R3239	T4915	T4916	advmod	approximately,500
R3240	T4916	T4917	nummod	500,mg
R3241	T4917	T4914	dobj	mg,taking
R3242	T4918	T4917	prep	of,mg
R3243	T4919	T4920	compound	liver,tissue
R3244	T4920	T4918	pobj	tissue,of
R3245	T4921	T4920	acl	cut,tissue
R3246	T4922	T4921	prep	into,cut
R3247	T4923	T4924	amod	small,pieces
R3248	T4924	T4922	pobj	pieces,into
R3249	T4925	T4921	cc	and,cut
R3250	T4926	T4921	conj	homogenized,cut
R3251	T4927	T4926	prep	in,homogenized
R3252	T4928	T4929	npadvmod	ice,cold
R3253	T4929	T4930	amod	cold,buffer
R3254	T4930	T4927	pobj	buffer,in
R3255	T4931	T4930	compound	lysis,buffer
R3256	T4932	T4933	punct	(,Tris
R3257	T4933	T4930	parataxis	Tris,buffer
R3258	T4934	T4935	nummod	5,mM
R3259	T4935	T4933	compound	mM,Tris
R3260	T4936	T4933	punct	-,Tris
R3261	T4937	T4933	npadvmod	pH,Tris
R3262	T4938	T4937	nummod	7.5,pH
R3263	T4939	T4933	punct	", ",Tris
R3264	T4940	T4941	nummod	250,mM
R3265	T4941	T4942	compound	mM,sucrose
R3266	T4942	T4933	appos	sucrose,Tris
R3267	T4943	T4933	prep	with,Tris
R3268	T4944	T4945	compound	protease,inhibitors
R3269	T4945	T4943	pobj	inhibitors,with
R3270	T4946	T4933	punct	),Tris
R3271	T4947	T4926	agent	by,homogenized
R3272	T4948	T4949	det	a,homogenizer
R3273	T4949	T4947	pobj	homogenizer,by
R3274	T4950	T4949	compound	dounce,homogenizer
R3275	T4951	T4949	prep	times,homogenizer
R3276	T4952	T4953	nummod	10,strokes
R3277	T4953	T4951	pobj	strokes,times
R3278	T4954	T4911	punct	.,prepared
R3279	T4956	T4957	nsubjpass	Samples,subjected
R3280	T4958	T4957	auxpass	were,subjected
R3281	T4959	T4957	advmod	then,subjected
R3282	T4960	T4957	prep	to,subjected
R3283	T4961	T4960	pobj	centrifugation,to
R3284	T4962	T4961	punct	–,centrifugation
R3285	T4963	T4964	nummod	1300,g
R3286	T4964	T4961	npadvmod	g,centrifugation
R3287	T4965	T4964	prep	for,g
R3288	T4966	T4967	nummod	10,minutes
R3289	T4967	T4965	pobj	minutes,for
R3290	T4968	T4964	prep	at,g
R3291	T4969	T4970	nummod	4,°C
R3292	T4970	T4968	pobj	°C,at
R3293	T4971	T4957	punct	.,subjected
R3294	T4973	T4974	det	The,supernant
R3295	T4974	T4975	nsubjpass	supernant,saved
R3296	T4976	T4975	auxpass	was,saved
R3297	T4977	T4975	cc	and,saved
R3298	T4978	T4979	det	the,pellet
R3299	T4979	T4980	nsubjpass	pellet,resuspended
R3300	T4980	T4975	conj	resuspended,saved
R3301	T4981	T4980	auxpass	was,resuspended
R3302	T4982	T4980	cc	and,resuspended
R3303	T4983	T4984	advmod	again,subjected
R3304	T4984	T4980	conj	subjected,resuspended
R3305	T4985	T4984	prep	to,subjected
R3306	T4986	T4987	compound	dounce,homogenization
R3307	T4987	T4985	pobj	homogenization,to
R3308	T4988	T4987	prep	times,homogenization
R3309	T4989	T4990	nummod	10,strokes
R3310	T4990	T4988	pobj	strokes,times
R3311	T4991	T4992	advmod	then,centrifuged
R3312	T4992	T4984	dep	centrifuged,subjected
R3313	T4993	T4994	nummod	1000,g
R3314	T4994	T4992	npadvmod	g,centrifuged
R3315	T4995	T4992	prep	for,centrifuged
R3316	T4996	T4997	nummod	10,minutes
R3317	T4997	T4995	pobj	minutes,for
R3318	T4998	T4992	prep	at,centrifuged
R3319	T4999	T5000	nummod	4,°C
R3320	T5000	T4998	pobj	°C,at
R3321	T5001	T4980	punct	.,resuspended
R3322	T5003	T5004	det	This,step
R3323	T5004	T5005	nsubjpass	step,repeated
R3324	T5006	T5005	auxpass	was,repeated
R3325	T5007	T5008	advmod	twice,time
R3326	T5008	T5005	npadvmod	time,repeated
R3327	T5009	T5008	det	each,time
R3328	T5010	T5005	prep	with,repeated
R3329	T5011	T5012	det	the,supernant
R3330	T5012	T5013	nsubjpass	supernant,collected
R3331	T5013	T5010	pcomp	collected,with
R3332	T5014	T5013	auxpass	being,collected
R3333	T5015	T5013	cc	and,collected
R3334	T5016	T5013	conj	kept,collected
R3335	T5017	T5016	oprd	on,kept
R3336	T5018	T5017	pobj	ice,on
R3337	T5019	T5005	punct	.,repeated
R3338	T5021	T5022	det	The,supernants
R3339	T5022	T5023	nsubjpass	supernants,pooled
R3340	T5024	T5023	auxpass	were,pooled
R3341	T5025	T5023	advmod	then,pooled
R3342	T5026	T5023	cc	and,pooled
R3343	T5027	T5023	conj	subjected,pooled
R3344	T5028	T5027	prep	to,subjected
R3345	T5029	T5028	pobj	ultracentrifugation,to
R3346	T5030	T5029	prep	at,ultracentrifugation
R3347	T5031	T5032	nummod	"100,000",g
R3348	T5032	T5030	pobj	g,at
R3349	T5033	T5027	prep	for,subjected
R3350	T5034	T5035	nummod	60,minutes
R3351	T5035	T5033	pobj	minutes,for
R3352	T5036	T5027	prep	at,subjected
R3353	T5037	T5038	nummod	4,°C
R3354	T5038	T5036	pobj	°C,at
R3355	T5039	T5023	punct	.,pooled
R3356	T5041	T5042	det	The,supernant
R3357	T5042	T5043	nsubjpass	supernant,removed
R3358	T5044	T5043	auxpass	was,removed
R3359	T5045	T5043	cc	and,removed
R3360	T5046	T5047	det	the,pellet
R3361	T5047	T5048	nsubjpass	pellet,resuspended
R3362	T5048	T5043	conj	resuspended,removed
R3363	T5049	T5048	auxpass	was,resuspended
R3364	T5050	T5048	prep	in,resuspended
R3365	T5051	T5052	compound	lysis,buffer
R3366	T5052	T5050	pobj	buffer,in
R3367	T5053	T5048	punct	.,resuspended
R3368	T5055	T5056	compound	Plasma,membranes
R3369	T5056	T5057	nsubjpass	membranes,prepared
R3370	T5058	T5057	auxpass	were,prepared
R3371	T5059	T5060	mark	as,described
R3372	T5060	T5057	advcl	described,prepared
R3373	T5061	T5060	advmod	previously,described
R3374	T5062	T5063	punct	[,31
R3375	T5063	T5057	parataxis	31,prepared
R3376	T5064	T5063	punct	],31
R3377	T5065	T5057	punct	.,prepared
R3378	T5067	T5068	compound	Protein,sample
R3379	T5068	T5069	compound	sample,concentrations
R3380	T5069	T5070	nsubjpass	concentrations,determined
R3381	T5071	T5070	auxpass	were,determined
R3382	T5072	T5070	advmod	then,determined
R3383	T5073	T5070	agent	by,determined
R3384	T5074	T5075	nmod	Bio,Rad
R3385	T5075	T5077	nmod	Rad,assay
R3386	T5076	T5075	punct	-,Rad
R3387	T5077	T5073	pobj	assay,by
R3388	T5078	T5079	npadvmod	protein,colormetric
R3389	T5079	T5077	amod	colormetric,assay
R3390	T5080	T5070	prep	per,determined
R3391	T5081	T5082	poss	manufacturer,protocol
R3392	T5082	T5080	pobj	protocol,per
R3393	T5083	T5081	case	's,manufacturer
R3394	T5084	T5085	punct	(,BioRad
R3395	T5085	T5082	parataxis	BioRad,protocol
R3396	T5086	T5085	punct	", ",BioRad
R3397	T5087	T5085	npadvmod	Hercules,BioRad
R3398	T5088	T5085	punct	", ",BioRad
R3399	T5089	T5085	npadvmod	CA,BioRad
R3400	T5090	T5085	punct	", ",BioRad
R3401	T5091	T5085	npadvmod	USA,BioRad
R3402	T5092	T5085	punct	),BioRad
R3403	T5093	T5070	punct	.,determined
R3404	T5095	T5096	nummod	50,μg
R3405	T5096	T5097	nsubjpass	μg,resolved
R3406	T5098	T5096	prep	of,μg
R3407	T5099	T5100	compound	membrane,proteins
R3408	T5100	T5098	pobj	proteins,of
R3409	T5101	T5097	auxpass	were,resolved
R3410	T5102	T5097	advmod	then,resolved
R3411	T5103	T5097	prep	on,resolved
R3412	T5104	T5105	nummod	7.5,%
R3413	T5105	T5106	compound	%,gel
R3414	T5106	T5103	pobj	gel,on
R3415	T5107	T5106	compound	SDS,gel
R3416	T5108	T5106	compound	acrylamide,gel
R3417	T5109	T5097	punct	.,resolved
R3418	T5168	T5169	nsubjpass	Proteins,transferred
R3419	T5170	T5168	acl	resolved,Proteins
R3420	T5171	T5170	agent	by,resolved
R3421	T5172	T5173	compound	SDS,PAGE
R3422	T5173	T5171	pobj	PAGE,by
R3423	T5174	T5173	punct	-,PAGE
R3424	T5175	T5169	auxpass	were,transferred
R3425	T5176	T5169	prep	to,transferred
R3426	T5177	T5178	compound	nitrocellulose,membranes
R3427	T5178	T5176	pobj	membranes,to
R3428	T5179	T5169	punct	.,transferred
R3429	T5181	T5182	nsubjpass	Membranes,blocked
R3430	T5183	T5182	auxpass	were,blocked
R3431	T5184	T5182	advmod	then,blocked
R3432	T5185	T5182	prep	for,blocked
R3433	T5186	T5187	nummod	one,hour
R3434	T5187	T5185	pobj	hour,for
R3435	T5188	T5182	prep	in,blocked
R3436	T5189	T5190	nummod	5,%
R3437	T5190	T5191	nmod	%,milk
R3438	T5191	T5188	pobj	milk,in
R3439	T5192	T5191	amod	dry,milk
R3440	T5193	T5182	prep	in,blocked
R3441	T5194	T5195	compound	PBS,T
R3442	T5195	T5193	pobj	T,in
R3443	T5196	T5195	punct	-,T
R3444	T5197	T5195	punct	(,T
R3445	T5198	T5199	npadvmod	Phosphate,Buffered
R3446	T5199	T5200	amod	Buffered,saline
R3447	T5200	T5195	appos	saline,T
R3448	T5201	T5200	cc	and,saline
R3449	T5202	T5203	nummod	0.1,%
R3450	T5203	T5204	compound	%,Tween
R3451	T5204	T5200	conj	Tween,saline
R3452	T5205	T5204	nummod	20,Tween
R3453	T5206	T5182	punct	),blocked
R3454	T5207	T5182	punct	.,blocked
R3455	T5209	T5210	nsubjpass	Blots,probed
R3456	T5211	T5210	auxpass	were,probed
R3457	T5212	T5210	advmod	then,probed
R3458	T5213	T5210	prep	with,probed
R3459	T5214	T5215	amod	primary,antibody
R3460	T5215	T5213	pobj	antibody,with
R3461	T5216	T5215	prep	against,antibody
R3462	T5217	T5216	pobj	Abcg5,against
R3463	T5218	T5210	prep	in,probed
R3464	T5219	T5220	nummod	5,%
R3465	T5220	T5221	compound	%,milk
R3466	T5221	T5218	pobj	milk,in
R3467	T5222	T5210	prep	in,probed
R3468	T5223	T5224	compound	PBS,T
R3469	T5224	T5222	pobj	T,in
R3470	T5225	T5224	punct	-,T
R3471	T5226	T5210	advmod	overnight,probed
R3472	T5227	T5210	prep	at,probed
R3473	T5228	T5229	nummod	4,°C
R3474	T5229	T5227	pobj	°C,at
R3475	T5230	T5210	punct	.,probed
R3476	T5232	T5233	det	The,blot
R3477	T5233	T5234	nsubjpass	blot,washed
R3478	T5235	T5234	auxpass	was,washed
R3479	T5236	T5234	advmod	then,washed
R3480	T5237	T5238	nummod	three,times
R3481	T5238	T5234	npadvmod	times,washed
R3482	T5239	T5234	prep	for,washed
R3483	T5240	T5241	nummod	five,minutes
R3484	T5241	T5239	pobj	minutes,for
R3485	T5242	T5234	prep	in,washed
R3486	T5243	T5244	compound	TBS,T
R3487	T5244	T5242	pobj	T,in
R3488	T5245	T5244	punct	-,T
R3489	T5246	T5244	punct	(,T
R3490	T5247	T5248	npadvmod	Tris,Buffered
R3491	T5248	T5249	amod	Buffered,Saline
R3492	T5249	T5244	appos	Saline,T
R3493	T5250	T5249	punct	/,Saline
R3494	T5251	T5252	nummod	0.1,%
R3495	T5252	T5253	compound	%,20
R3496	T5253	T5249	appos	20,Saline
R3497	T5254	T5253	compound	Tween,20
R3498	T5255	T5253	punct	-,20
R3499	T5256	T5234	punct	),washed
R3500	T5257	T5234	prep	with,washed
R3501	T5258	T5259	nummod	150,mM
R3502	T5259	T5260	compound	mM,NaCl
R3503	T5260	T5257	pobj	NaCl,with
R3504	T5261	T5234	punct	", ",washed
R3505	T5262	T5234	cc	and,washed
R3506	T5263	T5264	advmod	secondarily,stained
R3507	T5264	T5234	conj	stained,washed
R3508	T5265	T5264	prep	with,stained
R3509	T5266	T5267	compound	goat,anti-rabbit
R3510	T5267	T5269	npadvmod	anti-rabbit,conjugated
R3511	T5268	T5267	punct	-,anti-rabbit
R3512	T5269	T5270	amod	conjugated,dilution
R3513	T5270	T5265	pobj	dilution,with
R3514	T5271	T5270	nmod	HRP,dilution
R3515	T5272	T5270	nummod	1,dilution
R3516	T5273	T5274	punct	:,"10,000"
R3517	T5274	T5272	prep	"10,000",1
R3518	T5275	T5264	prep	in,stained
R3519	T5276	T5277	nummod	5,%
R3520	T5277	T5278	compound	%,milk
R3521	T5278	T5275	pobj	milk,in
R3522	T5279	T5264	prep	in,stained
R3523	T5280	T5281	compound	PBS,T
R3524	T5281	T5279	pobj	T,in
R3525	T5282	T5281	punct	-,T
R3526	T5283	T5264	prep	for,stained
R3527	T5284	T5285	nummod	one,hour
R3528	T5285	T5283	pobj	hour,for
R3529	T5286	T5234	punct	.,washed
R3530	T5288	T5289	det	The,blot
R3531	T5289	T5290	nsubjpass	blot,washed
R3532	T5291	T5290	auxpass	was,washed
R3533	T5292	T5290	advmod	again,washed
R3534	T5293	T5294	nummod	three,times
R3535	T5294	T5290	npadvmod	times,washed
R3536	T5295	T5290	prep	for,washed
R3537	T5296	T5297	nummod	five,minutes
R3538	T5297	T5295	pobj	minutes,for
R3539	T5298	T5290	prep	in,washed
R3540	T5299	T5300	compound	TBS,T
R3541	T5300	T5298	pobj	T,in
R3542	T5301	T5300	punct	-,T
R3543	T5302	T5290	prep	with,washed
R3544	T5303	T5304	nummod	150,mM
R3545	T5304	T5305	compound	mM,NaCl
R3546	T5305	T5302	pobj	NaCl,with
R3547	T5306	T5307	advmod	then,covered
R3548	T5307	T5290	dep	covered,washed
R3549	T5308	T5307	prep	in,covered
R3550	T5309	T5310	compound	Western,Lightning®
R3551	T5310	T5311	compound	Lightning®,Plus
R3552	T5311	T5308	pobj	Plus,in
R3553	T5312	T5311	compound	Chemiluminescence,Plus
R3554	T5313	T5311	compound	Reagent,Plus
R3555	T5314	T5315	punct	(,Sciences
R3556	T5315	T5311	parataxis	Sciences,Plus
R3557	T5316	T5315	compound	PerkinElmer,Sciences
R3558	T5317	T5315	compound	Life,Sciences
R3559	T5318	T5315	punct	", ",Sciences
R3560	T5319	T5315	amod	Inc.,Sciences
R3561	T5320	T5315	npadvmod	Boston,Sciences
R3562	T5321	T5315	punct	", ",Sciences
R3563	T5322	T5315	npadvmod	MA,Sciences
R3564	T5323	T5315	punct	", ",Sciences
R3565	T5324	T5315	npadvmod	USA,Sciences
R3566	T5325	T5315	punct	),Sciences
R3567	T5326	T5290	punct	", ",washed
R3568	T5327	T5290	conj	wrapped,washed
R3569	T5328	T5327	prep	in,wrapped
R3570	T5329	T5328	pobj	plastic,in
R3571	T5330	T5327	punct	", ",wrapped
R3572	T5331	T5327	conj	exposed,wrapped
R3573	T5332	T5331	prep	to,exposed
R3574	T5333	T5332	pobj	film,to
R3575	T5334	T5331	cc	and,exposed
R3576	T5335	T5331	conj	developed,exposed
R3577	T5336	T5290	punct	.,washed
R3578	T5424	T5425	amod	Immunohistochemical,analysis
R3579	T5427	T5428	advmod	Snap,frozen
R3580	T5428	T5430	amod	frozen,tissue
R3581	T5429	T5428	punct	-,frozen
R3582	T5430	T5434	nsubjpass	tissue,cut
R3583	T5431	T5430	nmod	liver,tissue
R3584	T5432	T5431	cc	and,liver
R3585	T5433	T5431	conj	intestine,liver
R3586	T5435	T5434	auxpass	were,cut
R3587	T5436	T5437	aux	to,produce
R3588	T5437	T5434	advcl	produce,cut
R3589	T5438	T5439	nummod	8,μm
R3590	T5439	T5440	npadvmod	μm,thick
R3591	T5440	T5441	amod	thick,sections
R3592	T5441	T5437	dobj	sections,produce
R3593	T5442	T5441	amod	frozen,sections
R3594	T5443	T5434	punct	", ",cut
R3595	T5444	T5445	dep	air,dried
R3596	T5445	T5434	conj	dried,cut
R3597	T5446	T5445	punct	-,dried
R3598	T5447	T5445	prep	for,dried
R3599	T5448	T5449	nummod	30,minutes
R3600	T5449	T5447	pobj	minutes,for
R3601	T5450	T5445	prep	onto,dried
R3602	T5451	T5452	compound	glass,slides
R3603	T5452	T5450	pobj	slides,onto
R3604	T5453	T5445	cc	and,dried
R3605	T5454	T5445	conj	stored,dried
R3606	T5455	T5454	prep	at,stored
R3607	T5456	T5457	punct	-,80
R3608	T5457	T5458	nummod	80,°C
R3609	T5458	T5455	pobj	°C,at
R3610	T5459	T5460	mark	until,required
R3611	T5460	T5454	advcl	required,stored
R3612	T5461	T5434	punct	.,cut
R3613	T5463	T5464	det	The,slides
R3614	T5464	T5465	nsubjpass	slides,stained
R3615	T5466	T5465	auxpass	were,stained
R3616	T5467	T5465	prep	with,stained
R3617	T5468	T5467	pobj	hematoxylin,with
R3618	T5469	T5465	punct	", ",stained
R3619	T5470	T5465	conj	rinsed,stained
R3620	T5471	T5472	nummod	three,times
R3621	T5472	T5470	npadvmod	times,rinsed
R3622	T5473	T5470	prep	with,rinsed
R3623	T5474	T5473	pobj	PBS,with
R3624	T5475	T5470	punct	", ",rinsed
R3625	T5476	T5470	conj	fixed,rinsed
R3626	T5477	T5476	prep	for,fixed
R3627	T5478	T5479	nummod	10,min
R3628	T5479	T5477	pobj	min,for
R3629	T5480	T5476	prep	with,fixed
R3630	T5481	T5482	amod	cooled,methanol
R3631	T5482	T5480	pobj	methanol,with
R3632	T5483	T5476	prep	at,fixed
R3633	T5484	T5485	punct	-,20
R3634	T5485	T5486	nummod	20,°C
R3635	T5486	T5483	pobj	°C,at
R3636	T5487	T5476	cc	and,fixed
R3637	T5488	T5489	advmod	then,rinsed
R3638	T5489	T5476	conj	rinsed,fixed
R3639	T5490	T5491	nummod	three,times
R3640	T5491	T5489	npadvmod	times,rinsed
R3641	T5492	T5489	prep	with,rinsed
R3642	T5493	T5492	pobj	PBS,with
R3643	T5494	T5465	punct	.,stained
R3644	T5496	T5497	nsubjpass	Slides,blocked
R3645	T5498	T5496	prep	for,Slides
R3646	T5499	T5500	compound	antibody,staining
R3647	T5500	T5498	pobj	staining,for
R3648	T5501	T5497	auxpass	were,blocked
R3649	T5502	T5497	advmod	initially,blocked
R3650	T5503	T5497	prep	in,blocked
R3651	T5504	T5505	nmod	PBS,M
R3652	T5505	T5508	nmod	M,serum
R3653	T5506	T5505	punct	/,M
R3654	T5507	T5505	nummod	0.1,M
R3655	T5508	T5503	pobj	serum,in
R3656	T5509	T5510	nmod	glycine,%
R3657	T5510	T5508	compound	%,serum
R3658	T5511	T5510	punct	/,%
R3659	T5512	T5510	nummod	10,%
R3660	T5513	T5508	compound	goat,serum
R3661	T5514	T5497	prep	for,blocked
R3662	T5515	T5516	nummod	60,min
R3663	T5516	T5514	pobj	min,for
R3664	T5517	T5497	prep	at,blocked
R3665	T5518	T5519	compound	room,temperature
R3666	T5519	T5517	pobj	temperature,at
R3667	T5520	T5521	advmod	then,incubated
R3668	T5521	T5497	dep	incubated,blocked
R3669	T5522	T5521	prep	with,incubated
R3670	T5523	T5524	amod	anti-sterolin,antibody
R3671	T5524	T5522	pobj	antibody,with
R3672	T5525	T5523	punct	-,anti-sterolin
R3673	T5526	T5523	advmod	1,anti-sterolin
R3674	T5527	T5528	punct	(,dilution
R3675	T5528	T5524	parataxis	dilution,antibody
R3676	T5529	T5528	nummod	1,dilution
R3677	T5530	T5531	punct	:,15
R3678	T5531	T5529	prep	15,1
R3679	T5532	T5528	punct	),dilution
R3680	T5533	T5521	advmod	overnight,incubated
R3681	T5534	T5521	prep	at,incubated
R3682	T5535	T5536	nummod	4,°C
R3683	T5536	T5534	pobj	°C,at
R3684	T5537	T5497	punct	.,blocked
R3685	T5539	T5540	det	The,slides
R3686	T5540	T5541	nsubj	slides,washed
R3687	T5542	T5541	aux	were,washed
R3688	T5543	T5541	prep	with,washed
R3689	T5544	T5545	nummod	1,PBS
R3690	T5545	T5543	pobj	PBS,with
R3691	T5546	T5544	punct	×,1
R3692	T5547	T5541	cc	and,washed
R3693	T5548	T5541	conj	incubated,washed
R3694	T5549	T5548	prep	with,incubated
R3695	T5550	T5551	amod	secondary,antibody
R3696	T5551	T5549	pobj	antibody,with
R3697	T5552	T5553	punct	(,dilution
R3698	T5553	T5551	parataxis	dilution,antibody
R3699	T5554	T5555	compound	goat,anti-rabbit
R3700	T5555	T5557	compound	anti-rabbit,IgG
R3701	T5556	T5555	punct	-,anti-rabbit
R3702	T5557	T5553	dep	IgG,dilution
R3703	T5558	T5557	acl	conjugated,IgG
R3704	T5559	T5558	prep	with,conjugated
R3705	T5560	T5559	pobj	rhodamine,with
R3706	T5561	T5553	punct	", ",dilution
R3707	T5562	T5553	nummod	1,dilution
R3708	T5563	T5564	punct	:,1000
R3709	T5564	T5562	prep	1000,1
R3710	T5565	T5553	punct	),dilution
R3711	T5566	T5548	prep	for,incubated
R3712	T5567	T5568	nummod	30,min
R3713	T5568	T5566	pobj	min,for
R3714	T5569	T5548	prep	at,incubated
R3715	T5570	T5571	compound	room,temperature
R3716	T5571	T5569	pobj	temperature,at
R3717	T5572	T5541	punct	", ",washed
R3718	T5573	T5541	dep	washed,washed
R3719	T5574	T5573	cc	and,washed
R3720	T5575	T5576	advmod	then,observed
R3721	T5576	T5573	conj	observed,washed
R3722	T5577	T5576	prep	under,observed
R3723	T5578	T5579	det	an,microscope
R3724	T5579	T5577	pobj	microscope,under
R3725	T5580	T5579	nmod	Olympus,microscope
R3726	T5581	T5579	nmod	BX,microscope
R3727	T5582	T5581	punct	-,BX
R3728	T5583	T5581	nummod	5,BX
R3729	T5584	T5579	amod	confocal,microscope
R3730	T5585	T5579	prep	with,microscope
R3731	T5586	T5585	pobj	Fluoview,with
R3732	T5587	T5541	punct	.,washed
R3733	T5589	T5590	det	The,sections
R3734	T5590	T5591	nsubjpass	sections,fixed
R3735	T5592	T5590	acl	stained,sections
R3736	T5593	T5592	prep	at,stained
R3737	T5594	T5595	det	the,Center
R3738	T5595	T5593	pobj	Center,at
R3739	T5596	T5595	compound	AMC,Center
R3740	T5597	T5595	compound	Liver,Center
R3741	T5598	T5591	auxpass	were,fixed
R3742	T5599	T5591	prep	in,fixed
R3743	T5600	T5601	nummod	100,%
R3744	T5601	T5602	compound	%,acetone
R3745	T5602	T5599	pobj	acetone,in
R3746	T5603	T5591	prep	for,fixed
R3747	T5604	T5605	nummod	30,min
R3748	T5605	T5603	pobj	min,for
R3749	T5606	T5591	prep	at,fixed
R3750	T5607	T5608	compound	room,temperature
R3751	T5608	T5606	pobj	temperature,at
R3752	T5609	T5591	punct	", ",fixed
R3753	T5610	T5591	dep	blocked,fixed
R3754	T5611	T5610	prep	in,blocked
R3755	T5612	T5613	det	a,solution
R3756	T5613	T5611	pobj	solution,in
R3757	T5614	T5613	prep	of,solution
R3758	T5615	T5616	nmod	PBS,%
R3759	T5616	T5614	pobj	%,of
R3760	T5617	T5616	punct	/,%
R3761	T5618	T5616	nummod	0.05,%
R3762	T5619	T5620	nmod	Tween,%
R3763	T5620	T5616	appos	%,%
R3764	T5621	T5619	punct	-,Tween
R3765	T5622	T5619	nummod	20,Tween
R3766	T5623	T5620	punct	/,%
R3767	T5624	T5620	nummod	5,%
R3768	T5625	T5626	compound	goat,serum
R3769	T5626	T5616	appos	serum,%
R3770	T5627	T5626	punct	(,serum
R3771	T5628	T5626	appos	GS,serum
R3772	T5629	T5626	punct	),serum
R3773	T5630	T5610	prep	for,blocked
R3774	T5631	T5632	nummod	one,hour
R3775	T5632	T5630	pobj	hour,for
R3776	T5633	T5591	punct	", ",fixed
R3777	T5634	T5635	advmod	then,incubated
R3778	T5635	T5591	dep	incubated,fixed
R3779	T5636	T5635	prep	with,incubated
R3780	T5637	T5636	pobj	Anti-sterolin,with
R3781	T5638	T5637	punct	-,Anti-sterolin
R3782	T5639	T5637	nummod	1,Anti-sterolin
R3783	T5640	T5641	punct	(,dilution
R3784	T5641	T5637	parataxis	dilution,Anti-sterolin
R3785	T5642	T5641	nummod	1,dilution
R3786	T5643	T5644	punct	:,40
R3787	T5644	T5642	prep	40,1
R3788	T5645	T5641	punct	),dilution
R3789	T5646	T5637	cc	and,Anti-sterolin
R3790	T5647	T5637	conj	anti-Bsep,Anti-sterolin
R3791	T5648	T5649	punct	(,dilution
R3792	T5649	T5647	parataxis	dilution,anti-Bsep
R3793	T5650	T5649	nummod	1,dilution
R3794	T5651	T5652	punct	:,200
R3795	T5652	T5650	prep	200,1
R3796	T5653	T5649	punct	),dilution
R3797	T5654	T5637	acl	diluted,Anti-sterolin
R3798	T5655	T5654	prep	in,diluted
R3799	T5656	T5657	compound	PBS,GS
R3800	T5657	T5655	pobj	GS,in
R3801	T5658	T5657	punct	/,GS
R3802	T5659	T5657	compound	Tween,GS
R3803	T5660	T5657	punct	/,GS
R3804	T5661	T5654	punct	", ",diluted
R3805	T5662	T5654	prep	for,diluted
R3806	T5663	T5664	nummod	one,hour
R3807	T5664	T5662	pobj	hour,for
R3808	T5665	T5591	punct	.,fixed
R3809	T5667	T5668	advmod	Thereafter,washed
R3810	T5669	T5668	punct	", ",washed
R3811	T5670	T5668	nsubjpass	slides,washed
R3812	T5671	T5668	auxpass	were,washed
R3813	T5672	T5673	nummod	3,min
R3814	T5673	T5668	npadvmod	min,washed
R3815	T5674	T5672	punct	×,3
R3816	T5675	T5673	nummod	10,min
R3817	T5676	T5668	prep	in,washed
R3818	T5677	T5678	compound	PBS,Tween
R3819	T5678	T5676	pobj	Tween,in
R3820	T5679	T5678	punct	/,Tween
R3821	T5680	T5668	cc	and,washed
R3822	T5681	T5682	det	the,antibody
R3823	T5682	T5684	nsubjpass	antibody,applied
R3824	T5683	T5682	amod	secondary,antibody
R3825	T5684	T5668	conj	applied,washed
R3826	T5685	T5682	punct	(,antibody
R3827	T5686	T5687	compound	goat,anti-rabbit
R3828	T5687	T5689	compound	anti-rabbit,Alexa
R3829	T5688	T5687	punct	-,anti-rabbit
R3830	T5689	T5682	appos	Alexa,antibody
R3831	T5690	T5689	nummod	488,Alexa
R3832	T5691	T5684	punct	),applied
R3833	T5692	T5684	auxpass	was,applied
R3834	T5693	T5684	prep	in,applied
R3835	T5694	T5695	nummod	1,dilution
R3836	T5695	T5693	pobj	dilution,in
R3837	T5696	T5697	punct	:,1000
R3838	T5697	T5694	prep	1000,1
R3839	T5698	T5684	prep	in,applied
R3840	T5699	T5700	compound	PBST,GS
R3841	T5700	T5698	pobj	GS,in
R3842	T5701	T5700	punct	/,GS
R3843	T5702	T5684	prep	for,applied
R3844	T5703	T5704	nummod	one,hour
R3845	T5704	T5702	pobj	hour,for
R3846	T5705	T5684	prep	at,applied
R3847	T5706	T5707	compound	room,temperature
R3848	T5707	T5705	pobj	temperature,at
R3849	T5708	T5684	punct	.,applied
R3850	T5710	T5711	prep	After,mounted
R3851	T5712	T5710	pcomp	washing,After
R3852	T5713	T5712	prep	for,washing
R3853	T5714	T5715	nummod	3,min
R3854	T5715	T5713	pobj	min,for
R3855	T5716	T5714	punct	×,3
R3856	T5717	T5715	nummod	10,min
R3857	T5718	T5719	det	the,sections
R3858	T5719	T5711	nsubjpass	sections,mounted
R3859	T5720	T5711	auxpass	were,mounted
R3860	T5721	T5711	prep	in,mounted
R3861	T5722	T5723	compound	Dapi,Dapco
R3862	T5723	T5725	compound	Dapco,solution
R3863	T5724	T5723	punct	/,Dapco
R3864	T5725	T5721	pobj	solution,in
R3865	T5726	T5711	punct	.,mounted
R3866	T5728	T5729	compound	Fluorescence,microscopy
R3867	T5729	T5730	nsubjpass	microscopy,done
R3868	T5731	T5730	auxpass	was,done
R3869	T5732	T5730	prep	with,done
R3870	T5733	T5734	det	a,microscope
R3871	T5734	T5732	pobj	microscope,with
R3872	T5735	T5734	compound	Leica,microscope
R3873	T5736	T5737	compound	DM,RA2
R3874	T5737	T5734	compound	RA2,microscope
R3875	T5738	T5734	acl	equipped,microscope
R3876	T5739	T5738	prep	with,equipped
R3877	T5740	T5741	det	a,camera
R3878	T5741	T5739	pobj	camera,with
R3879	T5742	T5741	compound	Leica,camera
R3880	T5743	T5741	compound	DC350F,camera
R3881	T5744	T5741	compound	photo,camera
R3882	T5745	T5730	punct	.,done
R3883	T5747	T5748	prep	For,used
R3884	T5749	T5750	compound	Dapi,staining
R3885	T5750	T5747	pobj	staining,For
R3886	T5751	T5748	punct	", ",used
R3887	T5752	T5753	nummod	359,nm
R3888	T5753	T5748	nsubjpass	nm,used
R3889	T5754	T5755	punct	(,exposure
R3890	T5755	T5753	parataxis	exposure,nm
R3891	T5756	T5757	nummod	30.38,ms
R3892	T5757	T5755	compound	ms,exposure
R3893	T5758	T5755	punct	),exposure
R3894	T5759	T5748	auxpass	was,used
R3895	T5760	T5748	cc	and,used
R3896	T5761	T5762	prep	for,used
R3897	T5762	T5748	conj	used,used
R3898	T5763	T5764	nmod	Abcg5,staining
R3899	T5764	T5761	pobj	staining,for
R3900	T5765	T5763	cc	and,Abcg5
R3901	T5766	T5763	conj	Bsep,Abcg5
R3902	T5767	T5762	punct	", ",used
R3903	T5768	T5769	nummod	514,nm
R3904	T5769	T5762	nsubjpass	nm,used
R3905	T5770	T5771	punct	(,exposure
R3906	T5771	T5769	parataxis	exposure,nm
R3907	T5772	T5771	prep	for,exposure
R3908	T5773	T5772	pobj	both,for
R3909	T5774	T5771	punct	: ,exposure
R3910	T5775	T5776	nummod	1654.34,ms
R3911	T5776	T5771	compound	ms,exposure
R3912	T5777	T5771	punct	),exposure
R3913	T5778	T5762	auxpass	was,used
R3914	T5779	T5762	cc	and,used
R3915	T5780	T5781	det	the,pictures
R3916	T5781	T5782	nsubjpass	pictures,analyzed
R3917	T5782	T5762	conj	analyzed,used
R3918	T5783	T5782	auxpass	were,analyzed
R3919	T5784	T5782	cc	and,analyzed
R3920	T5785	T5782	conj	overlaid,analyzed
R3921	T5786	T5785	advcl	using,overlaid
R3922	T5787	T5788	compound	FW4000,software
R3923	T5788	T5786	dobj	software,using
R3924	T5789	T5790	punct	(,Leica
R3925	T5790	T5788	parataxis	Leica,software
R3926	T5791	T5790	punct	),Leica
R3927	T5792	T5782	punct	.,analyzed
R3928	T5901	T5900	cc	and,Transfection
R3929	T5902	T5900	conj	immunostaining,Transfection
R3930	T5903	T5900	prep	of,Transfection
R3931	T5904	T5905	nmod	COS,cells
R3932	T5905	T5903	pobj	cells,of
R3933	T5906	T5904	punct	-,COS
R3934	T5907	T5904	nummod	1,COS
R3935	T5909	T5910	nmod	COS,cells
R3936	T5910	T5913	nsubjpass	cells,transfected
R3937	T5911	T5909	punct	-,COS
R3938	T5912	T5909	nummod	1,COS
R3939	T5914	T5913	auxpass	were,transfected
R3940	T5915	T5913	advmod	transiently,transfected
R3941	T5916	T5913	prep	with,transfected
R3942	T5917	T5918	nmod	pCMV,mouse
R3943	T5918	T5920	nmod	mouse,Abcg5
R3944	T5919	T5918	punct	-,mouse
R3945	T5920	T5921	nmod	Abcg5,constructs
R3946	T5921	T5916	pobj	constructs,with
R3947	T5922	T5920	cc	and,Abcg5
R3948	T5923	T5924	compound	pCMV,mouse
R3949	T5924	T5926	compound	mouse,Abcg8
R3950	T5925	T5924	punct	-,mouse
R3951	T5926	T5920	conj	Abcg8,Abcg5
R3952	T5927	T5921	compound	expression,constructs
R3953	T5928	T5913	advcl	using,transfected
R3954	T5929	T5930	det	the,reagent
R3955	T5930	T5928	dobj	reagent,using
R3956	T5931	T5930	compound	Qiagen,reagent
R3957	T5932	T5930	compound	SuperFect,reagent
R3958	T5933	T5913	prep	per,transfected
R3959	T5934	T5933	pobj	protocol,per
R3960	T5935	T5936	punct	(,Valencia
R3961	T5936	T5913	parataxis	Valencia,transfected
R3962	T5937	T5936	punct	", ",Valencia
R3963	T5938	T5936	npadvmod	CA,Valencia
R3964	T5939	T5936	punct	", ",Valencia
R3965	T5940	T5936	npadvmod	USA,Valencia
R3966	T5941	T5936	punct	),Valencia
R3967	T5942	T5913	punct	.,transfected
R3968	T5944	T5945	advmod	Briefly,seeded
R3969	T5946	T5945	punct	", ",seeded
R3970	T5947	T5948	nmod	COS,cells
R3971	T5948	T5945	nsubjpass	cells,seeded
R3972	T5949	T5947	punct	-,COS
R3973	T5950	T5947	nummod	1,COS
R3974	T5951	T5945	auxpass	were,seeded
R3975	T5952	T5945	prep	onto,seeded
R3976	T5953	T5954	det	a,plate
R3977	T5954	T5952	pobj	plate,onto
R3978	T5955	T5956	nummod	six,well
R3979	T5956	T5954	compound	well,plate
R3980	T5957	T5956	punct	-,well
R3981	T5958	T5959	det	the,day
R3982	T5959	T5945	npadvmod	day,seeded
R3983	T5960	T5961	amod	prior,to
R3984	T5961	T5959	prep	to,day
R3985	T5962	T5961	pobj	transfection,to
R3986	T5963	T5964	aux	to,generate
R3987	T5964	T5945	advcl	generate,seeded
R3988	T5965	T5966	nummod	60,%
R3989	T5966	T5967	compound	%,confluence
R3990	T5967	T5964	dobj	confluence,generate
R3991	T5968	T5969	advmod	when,grown
R3992	T5969	T5964	advcl	grown,generate
R3993	T5970	T5969	prep	at,grown
R3994	T5971	T5972	nummod	37,°C
R3995	T5972	T5970	pobj	°C,at
R3996	T5973	T5972	cc	and,°C
R3997	T5974	T5975	nummod	5,%
R3998	T5975	T5976	compound	%,CO2
R3999	T5976	T5972	conj	CO2,°C
R4000	T5977	T5969	prep	in,grown
R4001	T5978	T5977	pobj	DMEM,in
R4002	T5979	T5978	acl	supplemented,DMEM
R4003	T5980	T5979	prep	with,supplemented
R4004	T5981	T5982	nummod	10,%
R4005	T5982	T5983	compound	%,FBS
R4006	T5983	T5980	pobj	FBS,with
R4007	T5984	T5983	punct	", ",FBS
R4008	T5985	T5986	nummod	100,U
R4009	T5986	T5987	nmod	U,penicillin
R4010	T5987	T5983	conj	penicillin,FBS
R4011	T5988	T5989	punct	/,ml
R4012	T5989	T5986	prep	ml,U
R4013	T5990	T5987	punct	", ",penicillin
R4014	T5991	T5992	nummod	100,μg
R4015	T5992	T5993	nmod	μg,streptomycin
R4016	T5993	T5987	conj	streptomycin,penicillin
R4017	T5994	T5995	punct	/,ml
R4018	T5995	T5992	prep	ml,μg
R4019	T5996	T5993	cc	and,streptomycin
R4020	T5997	T5998	nummod	1,glutamine
R4021	T5998	T5993	conj	glutamine,streptomycin
R4022	T5999	T5997	punct	×,1
R4023	T6000	T5945	punct	.,seeded
R4024	T6002	T6003	prep	On,mixed
R4025	T6004	T6005	det	the,day
R4026	T6005	T6002	pobj	day,On
R4027	T6006	T6005	prep	of,day
R4028	T6007	T6006	pobj	transfection,of
R4029	T6008	T6003	punct	", ",mixed
R4030	T6009	T6010	nummod	2,μg
R4031	T6010	T6003	nsubjpass	μg,mixed
R4032	T6011	T6010	prep	of,μg
R4033	T6012	T6011	pobj	DNA,of
R4034	T6013	T6003	auxpass	was,mixed
R4035	T6014	T6003	prep	with,mixed
R4036	T6015	T6016	compound	Qiagen,SuperFect
R4037	T6016	T6017	compound	SuperFect,reagent
R4038	T6017	T6014	pobj	reagent,with
R4039	T6018	T6003	cc	and,mixed
R4040	T6019	T6003	conj	incubated,mixed
R4041	T6020	T6019	prep	for,incubated
R4042	T6021	T6022	nummod	10,minutes
R4043	T6022	T6020	pobj	minutes,for
R4044	T6023	T6003	punct	.,mixed
R4045	T6025	T6026	det	This,mixture
R4046	T6026	T6027	nsubjpass	mixture,added
R4047	T6028	T6027	auxpass	was,added
R4048	T6029	T6027	advmod	then,added
R4049	T6030	T6027	prep	to,added
R4050	T6031	T6032	npadvmod	serum,free
R4051	T6032	T6034	amod	free,media
R4052	T6033	T6032	punct	-,free
R4053	T6034	T6030	pobj	media,to
R4054	T6035	T6027	cc	and,added
R4055	T6036	T6027	conj	placed,added
R4056	T6037	T6036	prep	on,placed
R4057	T6038	T6039	det	the,cells
R4058	T6039	T6037	pobj	cells,on
R4059	T6040	T6041	aux	to,incubate
R4060	T6041	T6036	advcl	incubate,placed
R4061	T6042	T6041	prep	for,incubate
R4062	T6043	T6044	nummod	two,hours
R4063	T6044	T6042	pobj	hours,for
R4064	T6045	T6027	punct	.,added
R4065	T6047	T6048	det	The,cells
R4066	T6048	T6049	nsubjpass	cells,washed
R4067	T6050	T6049	auxpass	were,washed
R4068	T6051	T6049	advmod	once,washed
R4069	T6052	T6049	prep	with,washed
R4070	T6053	T6054	compound	1x,PBS
R4071	T6054	T6052	pobj	PBS,with
R4072	T6055	T6049	cc	and,washed
R4073	T6056	T6057	amod	fresh,media
R4074	T6057	T6058	nsubjpass	media,added
R4075	T6058	T6049	conj	added,washed
R4076	T6059	T6058	auxpass	was,added
R4077	T6060	T6058	punct	.,added
R4078	T6062	T6063	det	The,cells
R4079	T6063	T6065	nsubjpass	cells,incubated
R4080	T6064	T6063	amod	transfected,cells
R4081	T6066	T6065	auxpass	were,incubated
R4082	T6067	T6065	advmod	then,incubated
R4083	T6068	T6065	prep	for,incubated
R4084	T6069	T6070	nummod	48,hours
R4085	T6070	T6068	pobj	hours,for
R4086	T6071	T6072	aux	to,ensure
R4087	T6072	T6065	advcl	ensure,incubated
R4088	T6073	T6072	dobj	expression,ensure
R4089	T6074	T6065	cc	and,incubated
R4090	T6075	T6076	advmod	subsequently,fixed
R4091	T6076	T6065	conj	fixed,incubated
R4092	T6077	T6076	prep	with,fixed
R4093	T6078	T6079	det	a,mixture
R4094	T6079	T6077	pobj	mixture,with
R4095	T6080	T6079	nummod	1,mixture
R4096	T6081	T6082	punct	:,1
R4097	T6082	T6080	prep	1,1
R4098	T6083	T6079	prep	of,mixture
R4099	T6084	T6083	pobj	acetone,of
R4100	T6085	T6086	punct	:,methanol
R4101	T6086	T6084	prep	methanol,acetone
R4102	T6087	T6065	punct	.,incubated
R4103	T6089	T6090	prep	In,blocked
R4104	T6091	T6089	pobj	preparation,In
R4105	T6092	T6091	prep	for,preparation
R4106	T6093	T6092	pobj	immunostaining,for
R4107	T6094	T6090	punct	", ",blocked
R4108	T6095	T6090	nsubjpass	cells,blocked
R4109	T6096	T6090	auxpass	were,blocked
R4110	T6097	T6090	prep	with,blocked
R4111	T6098	T6099	nummod	2,%
R4112	T6099	T6100	compound	%,serum
R4113	T6100	T6097	pobj	serum,with
R4114	T6101	T6100	compound	goat,serum
R4115	T6102	T6090	prep	in,blocked
R4116	T6103	T6102	pobj	PBS,in
R4117	T6104	T6090	prep	for,blocked
R4118	T6105	T6106	nummod	30,minutes
R4119	T6106	T6104	pobj	minutes,for
R4120	T6107	T6090	punct	", ",blocked
R4121	T6108	T6109	advmod	then,incubated
R4122	T6109	T6090	conj	incubated,blocked
R4123	T6110	T6109	prep	with,incubated
R4124	T6111	T6112	nummod	1,dilution
R4125	T6112	T6110	pobj	dilution,with
R4126	T6113	T6114	punct	:,50
R4127	T6114	T6111	prep	50,1
R4128	T6115	T6112	prep	of,dilution
R4129	T6116	T6117	amod	primary,antibody
R4130	T6117	T6118	compound	antibody,anti-Abcg5
R4131	T6118	T6115	pobj	anti-Abcg5,of
R4132	T6119	T6109	prep	in,incubated
R4133	T6120	T6121	nummod	2,%
R4134	T6121	T6122	compound	%,PBS
R4135	T6122	T6119	pobj	PBS,in
R4136	T6123	T6124	compound	goat,serum
R4137	T6124	T6122	compound	serum,PBS
R4138	T6125	T6122	punct	-,PBS
R4139	T6126	T6109	prep	for,incubated
R4140	T6127	T6128	nummod	1.5,hours
R4141	T6128	T6126	pobj	hours,for
R4142	T6129	T6109	punct	", ",incubated
R4143	T6130	T6109	cc	and,incubated
R4144	T6131	T6132	advmod	subsequently,incubated
R4145	T6132	T6109	conj	incubated,incubated
R4146	T6133	T6132	prep	with,incubated
R4147	T6134	T6135	amod	secondary,antibody
R4148	T6135	T6133	pobj	antibody,with
R4149	T6136	T6135	compound	rhodamine,antibody
R4150	T6137	T6135	compound	anti-rabbit,antibody
R4151	T6138	T6135	compound	IgG,antibody
R4152	T6139	T6132	prep	for,incubated
R4153	T6140	T6141	nummod	30,minutes
R4154	T6141	T6139	pobj	minutes,for
R4155	T6142	T6090	punct	.,blocked
R4156	T6144	T6145	nsubjpass	Cells,observed
R4157	T6146	T6145	auxpass	were,observed
R4158	T6147	T6145	advmod	then,observed
R4159	T6148	T6145	prep	under,observed
R4160	T6149	T6150	det	an,microscope
R4161	T6150	T6148	pobj	microscope,under
R4162	T6151	T6150	nmod	Olympus,microscope
R4163	T6152	T6150	nmod	BX,microscope
R4164	T6153	T6152	punct	-,BX
R4165	T6154	T6152	nummod	5,BX
R4166	T6155	T6150	amod	confocal,microscope
R4167	T6156	T6150	prep	with,microscope
R4168	T6157	T6156	pobj	Fluoview,with
R4169	T6158	T6145	punct	.,observed
R4170	T6247	T6248	amod	Dynamic,lipid
R4171	T6249	T6248	amod	biliary,lipid
R4172	T6250	T6248	appos	collection,lipid
R4173	T6251	T6250	cc	and,collection
R4174	T6252	T6250	conj	analysis,collection
R4175	T6254	T6255	nsubjpass	Mice,anaesthetized
R4176	T6256	T6255	auxpass	were,anaesthetized
R4177	T6257	T6255	prep	by,anaesthetized
R4178	T6258	T6259	amod	intraperitoneal,injection
R4179	T6259	T6257	pobj	injection,by
R4180	T6260	T6259	prep	of,injection
R4181	T6261	T6262	nummod	1,mL
R4182	T6262	T6263	nmod	mL,fluanisone
R4183	T6263	T6260	pobj	fluanisone,of
R4184	T6264	T6265	punct	/,kg
R4185	T6265	T6262	prep	kg,mL
R4186	T6266	T6263	compound	fentanyl,fluanisone
R4187	T6267	T6263	punct	/,fluanisone
R4188	T6268	T6263	cc	and,fluanisone
R4189	T6269	T6270	nummod	10,mg
R4190	T6270	T6271	nmod	mg,Diazepam
R4191	T6271	T6263	conj	Diazepam,fluanisone
R4192	T6272	T6273	punct	/,kg
R4193	T6273	T6270	prep	kg,mg
R4194	T6274	T6255	punct	.,anaesthetized
R4195	T6276	T6277	det	The,abdomen
R4196	T6277	T6278	nsubjpass	abdomen,opened
R4197	T6279	T6278	auxpass	was,opened
R4198	T6280	T6278	cc	and,opened
R4199	T6281	T6278	punct	", ",opened
R4200	T6282	T6283	prep	after,cannulated
R4201	T6283	T6278	conj	cannulated,opened
R4202	T6284	T6285	amod	distal,ligation
R4203	T6285	T6282	pobj	ligation,after
R4204	T6286	T6285	prep	of,ligation
R4205	T6287	T6288	det	the,duct
R4206	T6288	T6286	pobj	duct,of
R4207	T6289	T6288	compound	bile,duct
R4208	T6290	T6283	punct	", ",cannulated
R4209	T6291	T6292	det	the,gallbladder
R4210	T6292	T6283	nsubjpass	gallbladder,cannulated
R4211	T6293	T6283	auxpass	was,cannulated
R4212	T6294	T6283	punct	.,cannulated
R4213	T6296	T6297	compound	Bile,sampling
R4214	T6297	T6298	nsubj	sampling,started
R4215	T6299	T6300	advmod	directly,after
R4216	T6300	T6298	prep	after,started
R4217	T6301	T6300	pobj	cannulation,after
R4218	T6302	T6298	cc	and,started
R4219	T6303	T6304	auxpass	was,collected
R4220	T6304	T6298	conj	collected,started
R4221	T6305	T6304	prep	for,collected
R4222	T6306	T6307	nummod	10,min
R4223	T6307	T6305	pobj	min,for
R4224	T6308	T6298	punct	.,started
R4225	T6310	T6311	compound	Bile,flow
R4226	T6311	T6312	nsubjpass	flow,determined
R4227	T6313	T6312	auxpass	was,determined
R4228	T6314	T6312	advmod	gravimetrically,determined
R4229	T6315	T6312	punct	.,determined
R4230	T6317	T6318	amod	Biliary,bile
R4231	T6318	T6319	nmod	bile,concentrations
R4232	T6319	T6325	nsubjpass	concentrations,determined
R4233	T6320	T6319	nmod	salt,concentrations
R4234	T6321	T6320	punct	", ",salt
R4235	T6322	T6320	conj	sterol,salt
R4236	T6323	T6322	cc	and,sterol
R4237	T6324	T6322	conj	phospholipid,sterol
R4238	T6326	T6325	auxpass	were,determined
R4239	T6327	T6328	mark	as,described
R4240	T6328	T6325	advcl	described,determined
R4241	T6329	T6328	advmod	previously,described
R4242	T6330	T6331	punct	[,32
R4243	T6331	T6325	parataxis	32,determined
R4244	T6332	T6331	punct	],32
R4245	T6333	T6325	punct	.,determined
R4246	T6335	T6336	aux	To,determine
R4247	T6336	T6337	advcl	determine,diverted
R4248	T6338	T6339	amod	maximal,rates
R4249	T6339	T6336	dobj	rates,determine
R4250	T6340	T6339	compound	secretion,rates
R4251	T6341	T6339	prep	of,rates
R4252	T6342	T6343	amod	biliary,lipid
R4253	T6343	T6344	compound	lipid,secretion
R4254	T6344	T6341	pobj	secretion,of
R4255	T6345	T6337	punct	", ",diverted
R4256	T6346	T6337	nsubjpass	bile,diverted
R4257	T6347	T6337	auxpass	was,diverted
R4258	T6348	T6337	prep	for,diverted
R4259	T6349	T6350	nummod	90,min
R4260	T6350	T6348	pobj	min,for
R4261	T6351	T6352	aux	to,deplete
R4262	T6352	T6337	advcl	deplete,diverted
R4263	T6353	T6354	det	the,pool
R4264	T6354	T6352	dobj	pool,deplete
R4265	T6355	T6354	amod	endogenous,pool
R4266	T6356	T6357	compound	bile,salt
R4267	T6357	T6354	compound	salt,pool
R4268	T6358	T6337	punct	.,diverted
R4269	T6360	T6361	advmod	Subsequently,infused
R4270	T6362	T6361	punct	", ",infused
R4271	T6363	T6361	nsubjpass	tauroursodeoxycholate,infused
R4272	T6364	T6365	punct	(,Co.
R4273	T6365	T6363	parataxis	Co.,tauroursodeoxycholate
R4274	T6366	T6365	compound	Sigma,Co.
R4275	T6367	T6365	compound	Chemical,Co.
R4276	T6368	T6365	punct	", ",Co.
R4277	T6369	T6365	npadvmod	MO,Co.
R4278	T6370	T6365	punct	", ",Co.
R4279	T6371	T6365	npadvmod	USA,Co.
R4280	T6372	T6365	punct	),Co.
R4281	T6373	T6363	acl	dissolved,tauroursodeoxycholate
R4282	T6374	T6373	prep	in,dissolved
R4283	T6375	T6374	pobj	PBS,in
R4284	T6376	T6361	auxpass	was,infused
R4285	T6377	T6361	prep	into,infused
R4286	T6378	T6379	det	the,vein
R4287	T6379	T6377	pobj	vein,into
R4288	T6380	T6379	amod	jugular,vein
R4289	T6381	T6361	prep	in,infused
R4290	T6382	T6383	amod	stepwise,rates
R4291	T6383	T6381	pobj	rates,in
R4292	T6384	T6383	amod	increasing,rates
R4293	T6385	T6361	prep	from,infused
R4294	T6386	T6387	quantmod	600,"1,800"
R4295	T6387	T6389	nummod	"1,800",nmol
R4296	T6388	T6387	punct	–,"1,800"
R4297	T6389	T6385	pobj	nmol,from
R4298	T6390	T6391	punct	/,min
R4299	T6391	T6389	prep	min,nmol
R4300	T6392	T6393	punct	/,weight
R4301	T6393	T6389	prep	weight,nmol
R4302	T6394	T6395	nummod	100,g
R4303	T6395	T6393	compound	g,weight
R4304	T6396	T6393	compound	body,weight
R4305	T6397	T6361	punct	.,infused
R4306	T6399	T6400	nsubjpass	Bile,collected
R4307	T6401	T6400	auxpass	was,collected
R4308	T6402	T6400	prep	in,collected
R4309	T6403	T6404	nummod	10,min
R4310	T6404	T6405	compound	min,fractions
R4311	T6405	T6402	pobj	fractions,in
R4312	T6406	T6400	cc	and,collected
R4313	T6407	T6400	conj	analyzed,collected
R4314	T6408	T6407	prep	for,analyzed
R4315	T6409	T6410	nmod	bile,content
R4316	T6410	T6408	pobj	content,for
R4317	T6411	T6410	nmod	salt,content
R4318	T6412	T6411	cc	and,salt
R4319	T6413	T6411	conj	lipid,salt
R4320	T6414	T6400	punct	.,collected
R4321	T6416	T6417	prep	In,diverted
R4322	T6418	T6419	amod	separate,experiments
R4323	T6419	T6416	pobj	experiments,In
R4324	T6420	T6417	punct	", ",diverted
R4325	T6421	T6417	prep	following,diverted
R4326	T6422	T6423	amod	distal,ligation
R4327	T6423	T6421	pobj	ligation,following
R4328	T6424	T6423	prep	of,ligation
R4329	T6425	T6426	det	the,duct
R4330	T6426	T6424	pobj	duct,of
R4331	T6427	T6426	compound	bile,duct
R4332	T6428	T6423	cc	and,ligation
R4333	T6429	T6423	conj	cannulation,ligation
R4334	T6430	T6429	prep	of,cannulation
R4335	T6431	T6430	pobj	gallbladder,of
R4336	T6432	T6417	punct	", ",diverted
R4337	T6433	T6417	nsubjpass	bile,diverted
R4338	T6434	T6417	auxpass	was,diverted
R4339	T6435	T6417	prep	for,diverted
R4340	T6436	T6437	nummod	30,minutes
R4341	T6437	T6435	pobj	minutes,for
R4342	T6438	T6439	mark	while,infused
R4343	T6439	T6417	advcl	infused,diverted
R4344	T6440	T6439	nsubjpass	mice,infused
R4345	T6441	T6439	auxpass	were,infused
R4346	T6442	T6439	prep	with,infused
R4347	T6443	T6444	amod	sterile,PBS
R4348	T6444	T6442	pobj	PBS,with
R4349	T6445	T6417	punct	.,diverted
R4350	T6447	T6448	advmod	Subsequently,infused
R4351	T6449	T6448	punct	", ",infused
R4352	T6450	T6451	det	the,mice
R4353	T6451	T6448	nsubjpass	mice,infused
R4354	T6452	T6448	auxpass	were,infused
R4355	T6453	T6448	advmod	continuously,infused
R4356	T6454	T6448	prep	with,infused
R4357	T6455	T6456	nummod	"1,200",nmol
R4358	T6456	T6454	pobj	nmol,with
R4359	T6457	T6458	punct	/,min
R4360	T6458	T6456	prep	min,nmol
R4361	T6459	T6460	punct	/,weight
R4362	T6460	T6456	prep	weight,nmol
R4363	T6461	T6462	nummod	100,g
R4364	T6462	T6460	compound	g,weight
R4365	T6463	T6460	compound	body,weight
R4366	T6464	T6456	prep	of,nmol
R4367	T6465	T6464	pobj	tauroursodeoxycholate,of
R4368	T6466	T6448	cc	and,infused
R4369	T6467	T6468	nummod	two,fractions
R4370	T6468	T6472	nsubjpass	fractions,collected
R4371	T6469	T6470	nummod	30,minute
R4372	T6470	T6468	compound	minute,fractions
R4373	T6471	T6470	punct	-,minute
R4374	T6472	T6448	conj	collected,infused
R4375	T6473	T6472	auxpass	were,collected
R4376	T6474	T6448	punct	.,infused
R4377	T6476	T6477	compound	Sterol,analyses
R4378	T6477	T6478	nsubjpass	analyses,performed
R4379	T6479	T6477	prep	of,analyses
R4380	T6480	T6479	pobj	bile,of
R4381	T6481	T6478	auxpass	was,performed
R4382	T6482	T6478	prep	by,performed
R4383	T6483	T6484	compound	GC,analysis
R4384	T6484	T6482	pobj	analysis,by
R4385	T6485	T6486	mark	as,described
R4386	T6486	T6478	advcl	described,performed
R4387	T6487	T6486	advmod	above,described
R4388	T6488	T6478	punct	.,performed
R4389	T6687	T6686	prep	of,Assay
R4390	T6688	T6689	compound	HMG,CoA
R4391	T6689	T6687	pobj	CoA,of
R4392	T6690	T6689	punct	-,CoA
R4393	T6691	T6686	cc	and,Assay
R4394	T6692	T6693	nmod	cholesterol,enzyme
R4395	T6693	T6698	compound	enzyme,activity
R4396	T6694	T6695	nummod	7,α
R4397	T6695	T6696	compound	α,hydroxylase
R4398	T6696	T6693	compound	hydroxylase,enzyme
R4399	T6697	T6696	punct	-,hydroxylase
R4400	T6698	T6686	conj	activity,Assay
R4401	T6700	T6701	compound	Mouse,liver
R4402	T6701	T6702	compound	liver,microsomes
R4403	T6702	T6703	nsubjpass	microsomes,prepared
R4404	T6704	T6703	auxpass	were,prepared
R4405	T6705	T6703	prep	by,prepared
R4406	T6706	T6707	amod	differential,centrifugation
R4407	T6707	T6705	pobj	centrifugation,by
R4408	T6708	T6709	punct	[,33
R4409	T6709	T6703	parataxis	33,prepared
R4410	T6710	T6709	punct	],33
R4411	T6711	T6703	punct	.,prepared
R4412	T6713	T6714	advmod	Briefly,homogenized
R4413	T6715	T6714	punct	", ",homogenized
R4414	T6716	T6717	quantmod	0.1,0.2
R4415	T6717	T6719	nummod	0.2,g
R4416	T6718	T6717	punct	–,0.2
R4417	T6719	T6720	compound	g,liver
R4418	T6720	T6714	nsubjpass	liver,homogenized
R4419	T6721	T6714	auxpass	was,homogenized
R4420	T6722	T6714	prep	in,homogenized
R4421	T6723	T6724	det	a,homogenizer
R4422	T6724	T6722	pobj	homogenizer,in
R4423	T6725	T6726	compound	Potter,Elvjhem
R4424	T6726	T6724	compound	Elvjhem,homogenizer
R4425	T6727	T6726	punct	-,Elvjhem
R4426	T6728	T6724	prep	with,homogenizer
R4427	T6729	T6730	nummod	five,volumes
R4428	T6730	T6728	pobj	volumes,with
R4429	T6731	T6730	prep	of,volumes
R4430	T6732	T6731	pobj	buffer,of
R4431	T6733	T6732	punct	(,buffer
R4432	T6734	T6735	nummod	0.25,M
R4433	T6735	T6736	compound	M,sucrose
R4434	T6736	T6732	appos	sucrose,buffer
R4435	T6737	T6736	punct	", ",sucrose
R4436	T6738	T6739	nummod	0.1,M
R4437	T6739	T6740	compound	M,Tris
R4438	T6740	T6736	appos	Tris,sucrose
R4439	T6741	T6736	punct	", ",sucrose
R4440	T6742	T6743	nummod	0.1,mM
R4441	T6743	T6744	compound	mM,EDTA
R4442	T6744	T6736	appos	EDTA,sucrose
R4443	T6745	T6744	compound	disodium,EDTA
R4444	T6746	T6736	punct	", ",sucrose
R4445	T6747	T6748	nummod	0.1,mM
R4446	T6748	T6749	compound	mM,DTT
R4447	T6749	T6736	appos	DTT,sucrose
R4448	T6750	T6736	punct	", ",sucrose
R4449	T6751	T6736	npadvmod	pH,sucrose
R4450	T6752	T6751	nummod	7.4,pH
R4451	T6753	T6714	punct	),homogenized
R4452	T6754	T6714	punct	.,homogenized
R4453	T6756	T6757	nsubjpass	Microsomes,isolated
R4454	T6758	T6757	auxpass	were,isolated
R4455	T6759	T6757	prep	by,isolated
R4456	T6760	T6761	amod	differential,centrifugation
R4457	T6761	T6759	pobj	centrifugation,by
R4458	T6762	T6763	punct	(,g
R4459	T6763	T6761	parataxis	g,centrifugation
R4460	T6764	T6763	nummod	"10,000",g
R4461	T6765	T6763	prep	to,g
R4462	T6766	T6767	nummod	"100,000",g
R4463	T6767	T6765	pobj	g,to
R4464	T6768	T6763	punct	),g
R4465	T6769	T6757	punct	.,isolated
R4466	T6771	T6772	det	The,pellets
R4467	T6772	T6773	nsubjpass	pellets,washed
R4468	T6774	T6773	auxpass	were,washed
R4469	T6775	T6773	cc	and,washed
R4470	T6776	T6773	conj	resuspended,washed
R4471	T6777	T6776	prep	in,resuspended
R4472	T6778	T6779	compound	storage,buffer
R4473	T6779	T6777	pobj	buffer,in
R4474	T6780	T6781	punct	(,phosphate
R4475	T6781	T6779	parataxis	phosphate,buffer
R4476	T6782	T6783	nummod	0.1,M
R4477	T6783	T6781	compound	M,phosphate
R4478	T6784	T6781	compound	dipotassium,phosphate
R4479	T6785	T6781	punct	", ",phosphate
R4480	T6786	T6787	nummod	0.05,M
R4481	T6787	T6788	compound	M,KCl
R4482	T6788	T6781	appos	KCl,phosphate
R4483	T6789	T6781	punct	", ",phosphate
R4484	T6790	T6791	nummod	1,mM
R4485	T6791	T6792	compound	mM,DTT
R4486	T6792	T6781	appos	DTT,phosphate
R4487	T6793	T6781	punct	", ",phosphate
R4488	T6794	T6795	nummod	5,mM
R4489	T6795	T6796	compound	mM,EDTA
R4490	T6796	T6781	appos	EDTA,phosphate
R4491	T6797	T6796	compound	disodium,EDTA
R4492	T6798	T6781	punct	", ",phosphate
R4493	T6799	T6800	nummod	20,%
R4494	T6800	T6801	compound	%,glycerol
R4495	T6801	T6781	appos	glycerol,phosphate
R4496	T6802	T6781	punct	", ",phosphate
R4497	T6803	T6781	npadvmod	pH,phosphate
R4498	T6804	T6803	nummod	7.4,pH
R4499	T6805	T6781	punct	),phosphate
R4500	T6806	T6776	prep	at,resuspended
R4501	T6807	T6808	quantmod	25,30
R4502	T6808	T6810	nummod	30,mg
R4503	T6809	T6808	punct	–,30
R4504	T6810	T6811	compound	mg,protein
R4505	T6811	T6806	pobj	protein,at
R4506	T6812	T6813	punct	/,ml
R4507	T6813	T6811	prep	ml,protein
R4508	T6814	T6773	punct	.,washed
R4509	T6816	T6817	compound	Protein,concentrations
R4510	T6817	T6818	nsubjpass	concentrations,determined
R4511	T6819	T6818	auxpass	were,determined
R4512	T6820	T6818	prep	according,determined
R4513	T6821	T6820	prep	to,according
R4514	T6822	T6823	det	the,method
R4515	T6823	T6821	pobj	method,to
R4516	T6824	T6823	prep	of,method
R4517	T6825	T6824	pobj	Lowry,of
R4518	T6826	T6827	advmod	et,al.
R4519	T6827	T6825	advmod	al.,Lowry
R4520	T6828	T6829	punct	[,34
R4521	T6829	T6818	parataxis	34,determined
R4522	T6830	T6829	punct	],34
R4523	T6831	T6818	punct	.,determined
R4524	T6833	T6834	det	The,activity
R4525	T6834	T6835	nsubjpass	activity,measured
R4526	T6836	T6834	prep	of,activity
R4527	T6837	T6838	nmod	cholesterol,hydroxylase
R4528	T6838	T6836	pobj	hydroxylase,of
R4529	T6839	T6840	nummod	7,α
R4530	T6840	T6838	compound	α,hydroxylase
R4531	T6841	T6838	punct	-,hydroxylase
R4532	T6842	T6843	punct	(,EC
R4533	T6843	T6838	parataxis	EC,hydroxylase
R4534	T6844	T6843	nummod	1.14.12.17,EC
R4535	T6845	T6843	punct	),EC
R4536	T6846	T6835	auxpass	was,measured
R4537	T6847	T6835	prep	by,measured
R4538	T6848	T6849	det	an,method
R4539	T6849	T6847	pobj	method,by
R4540	T6850	T6851	compound	isotope,incorporation
R4541	T6851	T6849	compound	incorporation,method
R4542	T6852	T6835	prep	according,measured
R4543	T6853	T6852	prep	to,according
R4544	T6854	T6853	pobj	Shefer,to
R4545	T6855	T6856	advmod	et,al.
R4546	T6856	T6854	advmod	al.,Shefer
R4547	T6857	T6858	punct	[,33
R4548	T6858	T6854	parataxis	33,Shefer
R4549	T6859	T6858	punct	],33
R4550	T6860	T6854	prep	with,Shefer
R4551	T6861	T6862	det	some,modifications
R4552	T6862	T6860	pobj	modifications,with
R4553	T6863	T6835	punct	.,measured
R4554	T6865	T6866	det	The,mixture
R4555	T6866	T6868	nsubj	mixture,consisted
R4556	T6867	T6866	compound	reaction,mixture
R4557	T6869	T6870	punct	(,ml
R4558	T6870	T6866	parataxis	ml,mixture
R4559	T6871	T6872	amod	final,volume
R4560	T6872	T6870	dep	volume,ml
R4561	T6873	T6870	nummod	0.5,ml
R4562	T6874	T6870	punct	),ml
R4563	T6875	T6868	prep	of,consisted
R4564	T6876	T6877	compound	potassium,phosphate
R4565	T6877	T6878	compound	phosphate,buffer
R4566	T6878	T6875	pobj	buffer,of
R4567	T6879	T6880	punct	(,K2HPO4
R4568	T6880	T6878	parataxis	K2HPO4,buffer
R4569	T6881	T6882	nummod	100,mM
R4570	T6882	T6880	compound	mM,K2HPO4
R4571	T6883	T6880	punct	", ",K2HPO4
R4572	T6884	T6885	nummod	0.1,mM
R4573	T6885	T6886	compound	mM,EDTA
R4574	T6886	T6880	appos	EDTA,K2HPO4
R4575	T6887	T6880	punct	", ",K2HPO4
R4576	T6888	T6889	nummod	5,mM
R4577	T6889	T6890	compound	mM,DTT
R4578	T6890	T6880	appos	DTT,K2HPO4
R4579	T6891	T6880	punct	", ",K2HPO4
R4580	T6892	T6893	nummod	30,mM
R4581	T6893	T6894	compound	mM,nicotinamide
R4582	T6894	T6880	appos	nicotinamide,K2HPO4
R4583	T6895	T6880	punct	", ",K2HPO4
R4584	T6896	T6880	npadvmod	pH,K2HPO4
R4585	T6897	T6896	nummod	7.4,pH
R4586	T6898	T6880	punct	),K2HPO4
R4587	T6899	T6878	punct	", ",buffer
R4588	T6900	T6901	punct	[,14C
R4589	T6901	T6878	parataxis	14C,buffer
R4590	T6902	T6901	punct	],14C
R4591	T6903	T6878	punct	-,buffer
R4592	T6904	T6878	appos	cholesterol,buffer
R4593	T6905	T6906	punct	(,dpm
R4594	T6906	T6904	parataxis	dpm,cholesterol
R4595	T6907	T6908	quantmod	5,105
R4596	T6908	T6906	nummod	105,dpm
R4597	T6909	T6908	punct	×,105
R4598	T6910	T6906	punct	),dpm
R4599	T6911	T6878	acl	solubilized,buffer
R4600	T6912	T6911	prep	in,solubilized
R4601	T6913	T6914	nummod	50,μl
R4602	T6914	T6912	pobj	μl,in
R4603	T6915	T6914	prep	of,μl
R4604	T6916	T6917	nummod	25,%
R4605	T6917	T6918	nmod	%,cyclodextrin
R4607	T6919	T6920	punct	(,vol
R4608	T6920	T6917	parataxis	vol,%
R4609	T6921	T6920	compound	wt,vol
R4610	T6922	T6920	punct	/,vol
R4611	T6923	T6920	punct	),vol
R4612	T6924	T6918	compound	β,cyclodextrin
R4613	T6925	T6918	punct	-,cyclodextrin
R4614	T6926	T6927	punct	(,%
R4615	T6927	T6918	parataxis	%,cyclodextrin
R4616	T6928	T6929	amod	final,concentration
R4617	T6929	T6927	dep	concentration,%
R4618	T6930	T6927	nummod	0.8,%
R4619	T6931	T6927	punct	),%
R4620	T6932	T6914	punct	", ",μl
R4621	T6933	T6914	cc	and,μl
R4622	T6934	T6935	quantmod	50,200
R4623	T6935	T6937	nummod	200,μg
R4624	T6936	T6935	punct	–,200
R4625	T6937	T6914	conj	μg,μl
R4626	T6938	T6937	prep	of,μg
R4627	T6939	T6940	amod	microsomal,protein
R4628	T6940	T6938	pobj	protein,of
R4629	T6941	T6868	punct	.,consisted
R4630	T6943	T6944	det	The,reaction
R4631	T6944	T6945	nsubjpass	reaction,initiated
R4632	T6946	T6945	auxpass	was,initiated
R4633	T6947	T6945	prep	by,initiated
R4634	T6948	T6949	det	the,addition
R4635	T6949	T6947	pobj	addition,by
R4636	T6950	T6949	prep	of,addition
R4637	T6951	T6950	pobj	NADPH,of
R4638	T6952	T6951	cc	or,NADPH
R4639	T6953	T6954	det	an,system
R4640	T6954	T6951	conj	system,NADPH
R4641	T6955	T6956	npadvmod	NADPH,generating
R4642	T6956	T6954	amod	generating,system
R4643	T6957	T6956	punct	-,generating
R4644	T6958	T6954	punct	(,system
R4645	T6959	T6960	nummod	3.4,mM
R4646	T6960	T6961	compound	mM,NADP+
R4647	T6961	T6954	appos	NADP+,system
R4648	T6962	T6961	punct	", ",NADP+
R4649	T6963	T6964	nummod	30,mM
R4650	T6964	T6965	nmod	mM,phosphate
R4651	T6965	T6961	appos	phosphate,NADP+
R4652	T6966	T6965	nmod	glucose,phosphate
R4653	T6967	T6966	punct	-,glucose
R4654	T6968	T6966	nummod	6,glucose
R4655	T6969	T6965	punct	-,phosphate
R4656	T6970	T6961	punct	", ",NADP+
R4657	T6971	T6972	nummod	0.3,U
R4658	T6972	T6961	appos	U,NADP+
R4659	T6973	T6972	prep	of,U
R4660	T6974	T6975	nmod	glucose,phosphate
R4661	T6975	T6979	compound	phosphate,dehydrogenase
R4662	T6976	T6974	punct	-,glucose
R4663	T6977	T6974	nummod	6,glucose
R4664	T6978	T6975	punct	-,phosphate
R4665	T6979	T6973	pobj	dehydrogenase,of
R4666	T6980	T6945	punct	),initiated
R4667	T6981	T6945	cc	and,initiated
R4668	T6982	T6945	conj	continued,initiated
R4669	T6983	T6982	prep	for,continued
R4670	T6984	T6985	nummod	15,min
R4671	T6985	T6983	pobj	min,for
R4672	T6986	T6982	prep	at,continued
R4673	T6987	T6988	nummod	37,°C
R4674	T6988	T6986	pobj	°C,at
R4675	T6989	T6945	punct	.,initiated
R4676	T6991	T6992	det	The,reaction
R4677	T6992	T6993	nsubjpass	reaction,stopped
R4678	T6994	T6993	auxpass	was,stopped
R4679	T6995	T6993	prep	with,stopped
R4680	T6996	T6997	nummod	0.5,ml
R4681	T6997	T6995	pobj	ml,with
R4682	T6998	T6997	prep	of,ml
R4683	T6999	T7000	nummod	1,N
R4684	T7000	T7001	compound	N,KOH
R4685	T7001	T6998	pobj	KOH,of
R4686	T7002	T6997	punct	", ",ml
R4687	T7003	T7004	nummod	5,μg
R4688	T7004	T6997	conj	μg,ml
R4689	T7005	T7004	prep	of,μg
R4690	T7006	T7007	amod	butylated,hydroxytoluene
R4691	T7007	T7005	pobj	hydroxytoluene,of
R4692	T7008	T7004	punct	", ",μg
R4693	T7009	T7004	cc	and,μg
R4694	T7010	T7011	nummod	10,μl
R4695	T7011	T7004	conj	μl,μg
R4696	T7012	T7011	prep	of,μl
R4697	T7013	T7014	amod	ethanolic,hydroxide
R4698	T7014	T7012	pobj	hydroxide,of
R4699	T7015	T7014	compound	potassium,hydroxide
R4700	T7016	T6993	punct	.,stopped
R4701	T7018	T7019	punct	[,3H
R4702	T7019	T7020	parataxis	3H,added
R4703	T7021	T7019	punct	],3H
R4704	T7022	T7023	nummod	7,α
R4705	T7023	T7024	compound	α,hydroxycholesterol
R4706	T7024	T7020	nsubjpass	hydroxycholesterol,added
R4707	T7025	T7024	punct	-,hydroxycholesterol
R4708	T7026	T7027	punct	(,μg
R4709	T7027	T7024	parataxis	μg,hydroxycholesterol
R4710	T7028	T7029	quantmod	1,104
R4711	T7029	T7027	nummod	104,μg
R4712	T7030	T7029	punct	×,104
R4713	T7031	T7027	nmod	dpm,μg
R4714	T7032	T7027	punct	/,μg
R4715	T7033	T7027	nummod	5,μg
R4716	T7034	T7027	punct	),μg
R4717	T7035	T7020	auxpass	was,added
R4718	T7036	T7020	prep	as,added
R4719	T7037	T7038	det	an,standard
R4720	T7038	T7036	pobj	standard,as
R4721	T7039	T7040	amod	internal,recovery
R4722	T7040	T7038	compound	recovery,standard
R4723	T7041	T7020	punct	.,added
R4724	T7043	T7044	prep	After,extracted
R4725	T7045	T7043	pobj	saponification,After
R4726	T7046	T7045	prep	at,saponification
R4727	T7047	T7048	nummod	37,°C
R4728	T7048	T7046	pobj	°C,at
R4729	T7049	T7045	prep	for,saponification
R4730	T7050	T7051	nummod	one,hour
R4731	T7051	T7049	pobj	hour,for
R4732	T7052	T7044	punct	", ",extracted
R4733	T7053	T7044	nsubjpass	sterols,extracted
R4734	T7054	T7044	auxpass	were,extracted
R4735	T7055	T7044	advmod	twice,extracted
R4736	T7056	T7044	prep	with,extracted
R4737	T7057	T7058	nummod	3,ml
R4738	T7058	T7056	pobj	ml,with
R4739	T7059	T7058	prep	of,ml
R4740	T7060	T7061	compound	n,hexane
R4741	T7061	T7059	pobj	hexane,of
R4742	T7062	T7061	punct	-,hexane
R4743	T7063	T7044	cc	and,extracted
R4744	T7064	T7065	det	the,extracts
R4745	T7065	T7066	nsubjpass	extracts,evaporated
R4746	T7066	T7044	conj	evaporated,extracted
R4747	T7067	T7066	auxpass	were,evaporated
R4748	T7068	T7066	prep	to,evaporated
R4749	T7069	T7068	pobj	dryness,to
R4750	T7070	T7066	prep	under,evaporated
R4751	T7071	T7070	pobj	nitrogen,under
R4752	T7072	T7044	punct	.,extracted
R4753	T7074	T7075	det	The,residue
R4754	T7075	T7076	nsubjpass	residue,dissolved
R4755	T7077	T7076	auxpass	was,dissolved
R4756	T7078	T7076	prep	in,dissolved
R4757	T7079	T7080	nummod	0.3,ml
R4758	T7080	T7078	pobj	ml,in
R4759	T7081	T7080	prep	of,ml
R4760	T7082	T7083	compound	n,hexane
R4761	T7083	T7081	pobj	hexane,of
R4762	T7084	T7083	punct	-,hexane
R4763	T7085	T7083	punct	:,hexane
R4764	T7086	T7087	nummod	2,propanol
R4765	T7087	T7083	appos	propanol,hexane
R4766	T7088	T7087	punct	-,propanol
R4767	T7089	T7090	punct	(,vol
R4768	T7090	T7080	parataxis	vol,ml
R4769	T7091	T7090	nummod	97.3,vol
R4770	T7092	T7090	compound	vol,vol
R4771	T7093	T7090	punct	/,vol
R4772	T7094	T7090	punct	),vol
R4773	T7095	T7076	cc	and,dissolved
R4774	T7096	T7076	conj	applied,dissolved
R4775	T7097	T7096	prep	to,applied
R4776	T7098	T7099	det	a,column
R4777	T7099	T7097	pobj	column,to
R4778	T7100	T7099	compound	silica,column
R4779	T7101	T7102	punct	(,pak
R4780	T7102	T7099	parataxis	pak,column
R4781	T7103	T7104	nummod	500,mg
R4782	T7104	T7102	dep	mg,pak
R4783	T7105	T7102	punct	;,pak
R4784	T7106	T7102	compound	sep,pak
R4785	T7107	T7102	punct	-,pak
R4786	T7108	T7102	punct	", ",pak
R4787	T7109	T7102	prep	by,pak
R4788	T7110	T7111	nummod	4,ml
R4789	T7111	T7109	pobj	ml,by
R4790	T7112	T7111	prep	of,ml
R4791	T7113	T7114	compound	n,hexane
R4792	T7114	T7112	pobj	hexane,of
R4793	T7115	T7114	punct	-,hexane
R4794	T7116	T7114	punct	:,hexane
R4795	T7117	T7118	nummod	2,propanol
R4796	T7118	T7114	appos	propanol,hexane
R4797	T7119	T7118	punct	-,propanol
R4798	T7120	T7121	punct	(,vol
R4799	T7121	T7111	parataxis	vol,ml
R4800	T7122	T7121	nummod	97.3,vol
R4801	T7123	T7121	compound	vol,vol
R4802	T7124	T7121	punct	/,vol
R4803	T7125	T7121	punct	),vol
R4804	T7126	T7102	punct	),pak
R4805	T7127	T7076	punct	.,dissolved
R4806	T7129	T7130	nummod	7,α
R4807	T7130	T7131	compound	α,hydroxycholesterol
R4808	T7131	T7133	nsubjpass	hydroxycholesterol,eluted
R4809	T7132	T7131	punct	-,hydroxycholesterol
R4810	T7134	T7133	auxpass	was,eluted
R4811	T7135	T7133	prep	with,eluted
R4812	T7136	T7137	nummod	3,ml
R4813	T7137	T7135	pobj	ml,with
R4814	T7138	T7137	prep	of,ml
R4815	T7139	T7140	compound	n,hexane
R4816	T7140	T7138	pobj	hexane,of
R4817	T7141	T7140	punct	-,hexane
R4818	T7142	T7140	punct	:,hexane
R4819	T7143	T7144	nummod	2,propanol
R4820	T7144	T7140	appos	propanol,hexane
R4821	T7145	T7144	punct	-,propanol
R4822	T7146	T7147	punct	(,vol
R4823	T7147	T7137	parataxis	vol,ml
R4824	T7148	T7147	dep	80,vol
R4825	T7149	T7150	punct	:,20
R4826	T7150	T7148	prep	20,80
R4827	T7151	T7152	punct	:,vol
R4828	T7152	T7147	prep	vol,vol
R4829	T7153	T7147	punct	),vol
R4830	T7154	T7133	cc	and,eluted
R4831	T7155	T7156	advmod	further,isolated
R4832	T7156	T7133	conj	isolated,eluted
R4833	T7157	T7156	prep	by,isolated
R4834	T7158	T7157	pobj	TLC,by
R4835	T7159	T7156	prep	on,isolated
R4836	T7160	T7161	compound	silica,gel
R4837	T7161	T7162	compound	gel,plates
R4838	T7162	T7159	pobj	plates,on
R4839	T7163	T7164	punct	(,Science
R4840	T7164	T7156	parataxis	Science,isolated
R4841	T7165	T7166	compound	Silica,gel
R4842	T7166	T7164	dep	gel,Science
R4843	T7167	T7166	nummod	60,gel
R4844	T7168	T7164	punct	", ",Science
R4845	T7169	T7164	compound	EM,Science
R4846	T7170	T7164	punct	", ",Science
R4847	T7171	T7164	npadvmod	Gibbestown,Science
R4848	T7172	T7164	punct	", ",Science
R4849	T7173	T7164	npadvmod	NJ,Science
R4850	T7174	T7164	punct	", ",Science
R4851	T7175	T7164	npadvmod	USA,Science
R4852	T7176	T7164	punct	),Science
R4853	T7177	T7156	prep	with,isolated
R4854	T7178	T7179	compound	diethyl,ether
R4855	T7179	T7177	pobj	ether,with
R4856	T7180	T7156	punct	", ",isolated
R4857	T7181	T7156	cc	and,isolated
R4858	T7182	T7156	conj	quantified,isolated
R4859	T7183	T7182	prep	by,quantified
R4860	T7184	T7185	compound	liquid,counting
R4861	T7185	T7183	pobj	counting,by
R4862	T7186	T7185	compound	scintillation,counting
R4863	T7187	T7182	advcl	using,quantified
R4864	T7188	T7187	dobj	Ecolume,using
R4865	T7189	T7190	punct	(,Radiochemicals
R4866	T7190	T7188	parataxis	Radiochemicals,Ecolume
R4867	T7191	T7190	compound	ICN,Radiochemicals
R4868	T7192	T7190	npadvmod	Irvine,Radiochemicals
R4869	T7193	T7190	punct	", ",Radiochemicals
R4870	T7194	T7190	npadvmod	CA,Radiochemicals
R4871	T7195	T7190	punct	", ",Radiochemicals
R4872	T7196	T7190	npadvmod	USA,Radiochemicals
R4873	T7197	T7190	punct	),Radiochemicals
R4874	T7198	T7133	punct	.,eluted
R4875	T7200	T7201	nsubjpass	Assays,carried
R4876	T7202	T7201	auxpass	were,carried
R4877	T7203	T7201	prt	out,carried
R4878	T7204	T7201	prep	in,carried
R4879	T7205	T7204	pobj	duplicate,in
R4880	T7206	T7201	prep	with,carried
R4881	T7207	T7206	pobj	correction,with
R4882	T7208	T7207	prep	for,correction
R4883	T7209	T7210	compound	zero,time
R4884	T7210	T7212	compound	time,controls
R4885	T7211	T7210	punct	-,time
R4886	T7212	T7208	pobj	controls,for
R4887	T7213	T7201	punct	.,carried
R4888	T7215	T7216	nmod	HMG,CoA
R4889	T7216	T7218	nmod	CoA,reductase
R4890	T7217	T7216	punct	-,CoA
R4891	T7218	T7219	nmod	reductase,activity
R4892	T7219	T7224	nsubjpass	activity,determined
R4893	T7220	T7221	punct	(,EC
R4894	T7221	T7218	parataxis	EC,reductase
R4895	T7222	T7221	nummod	1.1.1.3.4,EC
R4896	T7223	T7221	punct	),EC
R4897	T7225	T7224	auxpass	was,determined
R4898	T7226	T7224	prep	according,determined
R4899	T7227	T7226	prep	to,according
R4900	T7228	T7229	det	the,method
R4901	T7229	T7227	pobj	method,to
R4902	T7230	T7229	prep	of,method
R4903	T7231	T7230	pobj	Xu,of
R4904	T7232	T7233	advmod	et,al.
R4905	T7233	T7231	advmod	al.,Xu
R4906	T7234	T7235	punct	[,35
R4907	T7235	T7231	parataxis	35,Xu
R4908	T7236	T7235	punct	],35
R4909	T7237	T7224	prep	with,determined
R4910	T7238	T7237	pobj	modifications,with
R4911	T7239	T7240	mark	as,described
R4912	T7240	T7224	advcl	described,determined
R4913	T7241	T7242	punct	[,36
R4914	T7242	T7240	parataxis	36,described
R4915	T7243	T7242	punct	],36
R4916	T7244	T7224	punct	.,determined
R4917	T7248	T7249	amod	Statistical,analysis
R4918	T7251	T7252	nsubjpass	Data,shown
R4919	T7253	T7252	auxpass	are,shown
R4920	T7254	T7252	prep	as,shown
R4921	T7255	T7256	nmod	means,SD
R4922	T7256	T7254	pobj	SD,as
R4923	T7257	T7256	punct	±,SD
R4924	T7258	T7252	punct	.,shown
R4925	T7260	T7261	det	The,Student
R4926	T7261	T7262	poss	Student,test
R4927	T7262	T7265	nsubjpass	test,used
R4928	T7263	T7261	case	's,Student
R4929	T7264	T7262	compound	t,test
R4930	T7266	T7265	auxpass	was,used
R4931	T7267	T7268	aux	to,determine
R4932	T7268	T7265	advcl	determine,used
R4933	T7269	T7270	det	the,significance
R4934	T7270	T7268	dobj	significance,determine
R4935	T7271	T7270	amod	statistical,significance
R4936	T7272	T7270	prep	of,significance
R4937	T7273	T7272	pobj	differences,of
R4938	T7274	T7273	prep	between,differences
R4939	T7275	T7276	det	the,groups
R4940	T7276	T7274	pobj	groups,between
R4941	T7277	T7276	prep	of,groups
R4942	T7278	T7277	pobj	animals,of
R4943	T7279	T7265	punct	.,used
R4944	T7281	T7282	nsubjpass	Significance,set
R4945	T7283	T7282	auxpass	was,set
R4946	T7284	T7282	prep	at,set
R4947	T7285	T7286	compound	P,values
R4948	T7286	T7284	pobj	values,at
R4949	T7287	T7288	punct	<,0.05
R4950	T7288	T7286	amod	0.05,values
R4951	T7289	T7282	punct	.,set
R4954	T7461	T7460	prep	of,Isolation
R4955	T7462	T7463	compound	Abcg8,sterolin
R4956	T7463	T7465	npadvmod	sterolin,deficient
R4957	T7464	T7463	punct	/,sterolin
R4958	T7465	T7468	amod	deficient,mice
R4959	T7466	T7463	punct	-,sterolin
R4960	T7467	T7463	nummod	2,sterolin
R4961	T7468	T7461	pobj	mice,of
R4962	T7470	T7471	det	The,construct
R4963	T7471	T7473	nsubj	construct,results
R4964	T7472	T7471	amod	targeting,construct
R4965	T7474	T7475	punct	(,1a
R4966	T7475	T7471	parataxis	1a,construct
R4967	T7476	T7475	compound	Figure,1a
R4968	T7477	T7475	punct	),1a
R4969	T7478	T7473	prep	in,results
R4970	T7479	T7480	det	the,disruption
R4971	T7480	T7478	pobj	disruption,in
R4972	T7481	T7480	amod	potential,disruption
R4973	T7482	T7480	prep	of,disruption
R223	T505	T504	amod	apical,expression
R232	T516	T515	amod	impaired,ability
R252	T538	T537	amod	intermediate,phenotype
R256	T542	T541	amod	heterozygous,mice
R257	T543	T541	nmod	Abcg8,mice
R258	T544	T543	punct	+,Abcg8
R259	T545	T543	punct	/,Abcg8
R260	T546	T543	punct	-,Abcg8
R270	T556	T555	amod	decreased,level
R274	T560	T559	compound	sterol,secretion
R279	T565	T564	punct	-,type
R286	T576	T577	compound	Abcg8,sterolin
R289	T579	T577	punct	-,sterolin
R290	T580	T577	nummod	2,sterolin
R295	T585	T584	compound	sterol,secretion
R299	T589	T588	nsubj	loss,has
R300	T590	T589	prep	of,loss
R301	T591	T592	compound	Abcg8,sterolin
R304	T594	T592	punct	-,sterolin
R305	T595	T592	nummod	2,sterolin
R308	T598	T599	advmod	more,profound
R309	T599	T597	amod	profound,effect
R313	T603	T602	compound	cholesterol,secretion
R319	T610	T611	amod	biliary,secretion
R322	T613	T609	auxpass	is,preserved
R326	T618	T617	neg	not,elevated
R330	T622	T621	amod	sitosterolemic,mice
R336	T628	T627	nsubj	mechanisms,be
R337	T629	T628	amod	other,mechanisms
R338	T630	T629	prep	than,other
R339	T631	T630	prep	by,than
R340	T632	T633	compound	Abcg8,sterolin
R343	T635	T633	punct	-,sterolin
R344	T636	T633	nummod	2,sterolin
R345	T637	T627	aux	may,be
R9586	T14813	T14814	det	The,history
R9587	T14814	T14816	nsubjpass	history,accessed
R9588	T14815	T14814	amod	pre-publication,history
R3169	T4787	T4795	nsubjpass	antibodies,raised
R2088	T3291	T3289	pobj	probe,of
R2229	T3520	T3518	pobj	System,to
R2	T260	T258	compound	mouse,model
R10	T269	T268	punct	/,sterolin
R28	T292	T291	compound	STSL,locus
R32	T296	T295	punct	-,binding
R35	T299	T297	punct	(,cassette
R36	T300	T297	appos	ABC,cassette
R37	T301	T298	punct	),transporters
R38	T302	T298	punct	-,transporters
R60	T324	T323	amod	rare,disorder
R61	T325	T326	amod	autosomal,recessive
R62	T326	T323	amod	recessive,disorder
R70	T334	T333	compound	plasma,levels
R71	T335	T336	compound	plant,sterol
R72	T336	T333	compound	sterol,levels
R83	T349	T348	punct	/,sterolin
R89	T355	T354	punct	/,sterolin
R101	T368	T367	amod	phenotypic,difference
R131	T401	T400	compound	sterol,entry
R135	T405	T404	compound	sterol,secretion
R143	T417	T416	amod	human,disease
R150	T424	T423	amod	targeted,disruption
R154	T428	T427	compound	mouse,model
R161	T435	T434	punct	/,sterolin
R163	T437	T434	punct	-,sterolin
R164	T438	T434	nummod	2,sterolin
R169	T444	T443	compound	knockout,mice
R185	T465	T464	compound	plant,sterol
R200	T482	T481	punct	/,sterolin
R212	T494	T493	punct	/,sterolin
R214	T496	T493	punct	-,sterolin
R215	T497	T493	nummod	2,sterolin
R1450	T2443	T2440	parataxis	line,cells
R1610	T2613	T2608	parataxis	males,mice
R2705	T4108	T4106	dobj	values,subtracting
R4606	T6918	T6915	pobj	cyclodextrin,of
R538	T1268	T1257	attr	disorder,is
R547	T1277	T1275	pobj	levels,by
R619	T1353	T1351	pobj	levels,with
R686	T1424	T1403	conj	is,leads
R9144	T14301	T14299	punct	-,sterolin
R9145	T14302	T14299	nummod	1,sterolin
R9147	T14303	T14292	auxpass	was,used
R9148	T14304	T14292	punct	.,used
R9400	T14604	T14603	amod	other,proteins
R9402	T14605	T14604	prep	than,other
R9403	T14606	T14607	det	the,sterolins
R9405	T14607	T14605	pobj	sterolins,than
R9409	T14608	T14609	dep	that,secrete
R9410	T14609	T14603	relcl	secrete,proteins
R9412	T14610	T14609	aux	can,secrete
R9413	T14611	T14612	amod	biliary,sterols
R9414	T14612	T14609	dobj	sterols,secrete
R9415	T14613	T14576	punct	.,suggest

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T159	2-7	NCBITaxon:10088	denotes	mouse
T160	44-49	PR_EXT:000003573	denotes	Abcg8
T161	50-60	PR_EXT:000003573	denotes	sterolin-2
T162	83-90	GO:0046903	denotes	secrete
T163	91-98	UBERON:0002294	denotes	biliary
T164	99-110	CHEBI:16113	denotes	cholesterol
T165	132-141	SO_EXT:sequence_alteration_entity_or_process	denotes	Mutations
T166	159-164	SO_EXT:0000704	denotes	genes
T167	192-195	CHEBI_EXT:ATP	denotes	ATP
T168	196-203	CHEMINF_GO_EXT:chemical_binding_or_bond_formation	denotes	binding
T169	219-231	GO_EXT:biological_mover_or_transporter	denotes	transporters
T170	232-237	PR_EXT:000003572	denotes	ABCG5
T171	239-247	SO_EXT:sequence_coding_function	denotes	encoding
T172	248-258	PR_EXT:000003572	denotes	sterolin-1
T173	264-269	PR_EXT:000003573	denotes	ABCG8
T174	271-279	SO_EXT:sequence_coding_function	denotes	encoding
T175	280-290	PR_EXT:000003573	denotes	sterolin-2
T176	326-335	GO:0030849	denotes	autosomal
T177	358-364	CHEBI:15889	denotes	sterol
T178	358-376	GO:0015918	denotes	sterol trafficking
T179	404-410	UBERON:0001969	denotes	plasma
T180	411-416	NCBITaxon:3193	denotes	plant
T181	417-423	CHEBI:15889	denotes	sterol
T182	447-455	OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process	denotes	genetics
T183	475-480	PR_EXT:000003572	denotes	ABCG5
T184	481-491	PR_EXT:000003572	denotes	sterolin-1
T185	496-501	PR_EXT:000003573	denotes	ABCG8
T186	502-512	PR_EXT:000003573	denotes	sterolin-2
T187	560-566	GO_EXT:dimer_macromolecular_complex	denotes	dimers
T188	619-625	NCBITaxon:9606	denotes	humans
T189	658-663	PR_EXT:000003572	denotes	ABCG5
T190	667-672	PR_EXT:000003573	denotes	ABCG8
T191	680-688	CHEBI_PR_EXT:protein	denotes	proteins
T192	716-722	NCBITaxon:9606	denotes	humans
T193	744-754	GO:0065007	denotes	regulating
T194	763-769	CHEBI:15889	denotes	sterol
T195	763-775	GO:0035376	denotes	sterol entry
T196	780-787	UBERON:0002294	denotes	biliary
T197	788-794	CHEBI:15889	denotes	sterol
T198	795-804	GO_EXT:secretion_entity_or_process	denotes	secretion
T199	837-842	NCBITaxon:9606	denotes	human
T200	892-897	NCBITaxon:10088	denotes	mouse
T201	935-940	PR_EXT:000003573	denotes	Abcg8
T202	941-951	PR_EXT:000003573	denotes	sterolin-2
T203	990-994	NCBITaxon:10088	denotes	mice
T204	1043-1047	NCBITaxon:10088	denotes	Mice
T205	1061-1066	PR_EXT:000003573	denotes	Abcg8
T206	1096-1102	UBERON:0001969	denotes	plasma
T207	1107-1113	UBERON:0000479	denotes	tissue
T208	1114-1119	NCBITaxon:3193	denotes	plant
T209	1120-1126	CHEBI:15889	denotes	sterol
T210	1135-1145	CHEBI:27693	denotes	sitosterol
T211	1150-1161	CHEBI:28623	denotes	campesterol
T212	1210-1215	PR_EXT:000003572	denotes	Abcg5
T213	1216-1226	PR_EXT:000003572	denotes	sterolin-1
T214	1231-1240	GO:0010467	denotes	expressed
T215	1249-1254	UBERON:0002107	denotes	liver
T216	1259-1268	UBERON:0000160	denotes	intestine
T217	1272-1277	PR_EXT:000003573	denotes	Abcg8
T218	1278-1288	PR_EXT:000003573	denotes	sterolin-2
T219	1299-1303	NCBITaxon:10088	denotes	mice
T220	1329-1335	GO:0045177	denotes	apical
T221	1336-1346	GO:0010467	denotes	expression
T222	1360-1365	PR_EXT:000003573	denotes	Abcg8
T223	1376-1380	NCBITaxon:10088	denotes	mice
T224	1408-1415	GO:0046903	denotes	secrete
T225	1416-1427	CHEBI:16113	denotes	cholesterol
T226	1433-1437	UBERON:0001970	denotes	bile
T227	1475-1482	GO:0046903	denotes	secrete
T228	1483-1493	CHEBI:27693	denotes	sitosterol
T229	1556-1561	PR_EXT:000003573	denotes	Abcg8
T230	1561-1562	SO_EXT:normal_or_wild_type_or_present	denotes	+
T231	1563-1564	SO_EXT:sequence_nullness_or_absence	denotes	-
T232	1565-1569	NCBITaxon:10088	denotes	mice
T233	1629-1636	UBERON:0002294	denotes	biliary
T234	1637-1643	CHEBI:15889	denotes	sterol
T235	1644-1653	GO_EXT:secretion_entity_or_process	denotes	secretion
T236	1666-1675	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T237	1676-1680	NCBITaxon:10088	denotes	mice
T238	1719-1724	PR_EXT:000003573	denotes	Abcg8
T239	1725-1735	PR_EXT:000003573	denotes	sterolin-2
T240	1753-1760	UBERON:0002294	denotes	biliary
T241	1761-1767	CHEBI:15889	denotes	sterol
T242	1768-1777	GO_EXT:secretion_entity_or_process	denotes	secretion
T243	1795-1800	PR_EXT:000003573	denotes	Abcg8
T244	1801-1811	PR_EXT:000003573	denotes	sterolin-2
T245	1844-1851	UBERON:0002294	denotes	biliary
T246	1852-1863	CHEBI:16113	denotes	cholesterol
T247	1864-1873	GO_EXT:secretion_entity_or_process	denotes	secretion
T248	1879-1889	CHEBI:27693	denotes	sitosterol
T249	1897-1904	UBERON:0002294	denotes	biliary
T250	1905-1915	CHEBI:27693	denotes	sitosterol
T251	1916-1925	GO_EXT:secretion_entity_or_process	denotes	secretion
T252	1984-1988	NCBITaxon:10088	denotes	mice
T253	2046-2051	PR_EXT:000003573	denotes	Abcg8
T254	2052-2062	PR_EXT:000003573	denotes	sterolin-2
T255	2090-2099	GO_EXT:secretion_entity_or_process	denotes	secretion
T256	2105-2109	UBERON:0001970	denotes	bile
T911	2123-2136	_FRAGMENT	denotes	Absorption of
T912	2145-2161	_FRAGMENT	denotes	cholesterol from
T913	2166-2175	GO:0030299	denotes	intestine
T914	2145-2156	CHEBI:16113	denotes	cholesterol
T915	2166-2175	UBERON:0000160	denotes	intestine
T916	2200-2211	CHEBI:16113	denotes	cholesterol
T917	2200-2223	GO:0042632	denotes	cholesterol homeostasis
T918	2276-2287	CHEBI:16113	denotes	cholesterol
T919	2301-2310	GO:0008152	denotes	metabolic
T920	2380-2391	CHEBI:16113	denotes	cholesterol
T921	2400-2411	CHEBI:16113	denotes	cholesterol
T922	2412-2419	CHEBI:15889	denotes	sterols
T923	2428-2433	NCBITaxon:3193	denotes	plant
T924	2434-2441	CHEBI:15889	denotes	sterols
T925	2477-2488	CHEBI:16113	denotes	cholesterol
T926	2549-2560	CHEBI:16113	denotes	cholesterol
T927	2561-2568	CHEBI:15889	denotes	sterols
T928	2633-2644	CHEBI:16113	denotes	cholesterol
T929	2654-2665	CHEBI:16113	denotes	cholesterol
T930	2666-2673	CHEBI:15889	denotes	sterols
T931	2694-2703	UBERON:0000160	denotes	intestine
T932	2724-2733	CHEBI_EXT:polyatomic_entity_or_group	denotes	molecular
T933	2767-2778	CHEBI:16113	denotes	cholesterol
T934	2856-2861	UBERON:0002107	denotes	liver
T935	2862-2870	GO:0046903	denotes	secretes
T936	2876-2887	CHEBI:16113	denotes	cholesterol
T937	2893-2897	UBERON:0001970	denotes	bile
T938	2921-2928	GO:0007588	denotes	excrete
T939	2933-2944	CHEBI:16113	denotes	cholesterol
T940	2945-2952	CHEBI:15889	denotes	sterols
T941	2958-2962	UBERON:0001970	denotes	bile
T942	3077-3082	NCBITaxon:9606	denotes	human
T943	3203-3212	GO:0030849	denotes	autosomal
T944	3271-3276	UBERON:0000178	denotes	blood
T945	3281-3287	UBERON:0000479	denotes	tissue
T946	3288-3293	NCBITaxon:3193	denotes	plant
T947	3294-3300	CHEBI:15889	denotes	sterol
T948	3322-3333	NCBITaxon:1	denotes	individuals
T949	3346-3352	UBERON:0000043	denotes	tendon
T950	3360-3368	UBERON:4200132	denotes	tuberous
T951	3514-3520	UBERON:0001969	denotes	plasma
T952	3521-3526	NCBITaxon:3193	denotes	plant
T953	3527-3533	CHEBI:15889	denotes	sterol
T954	3555-3565	CHEBI:27693	denotes	sitosterol
T955	3567-3578	CHEBI:28623	denotes	campesterol
T956	3580-3592	CHEBI:28824	denotes	stigmasterol
T957	3646-3657	CHEBI_EXT:25212	denotes	metabolites
T958	3725-3736	CHEBI:16113	denotes	cholesterol
T959	3787-3798	NCBITaxon:1	denotes	individuals
T960	3823-3830	CHEBI:15889	denotes	sterols
T961	3875-3881	CHEBI:15889	denotes	sterol
T962	3929-3936	GO:0007588	denotes	excrete
T963	3937-3944	CHEBI:15889	denotes	sterols
T964	3950-3954	UBERON:0001970	denotes	bile
T965	3974-3984	CHEBI:27693	denotes	sitosterol
T966	3989-4000	CHEBI:16113	denotes	cholesterol
T967	4013-4024	NCBITaxon:1	denotes	individuals
T968	4059-4068	GO:0007588	denotes	excretion
T969	4072-4082	CHEBI:27693	denotes	sitosterol
T970	4088-4092	UBERON:0001970	denotes	bile
T971	4116-4127	NCBITaxon:1	denotes	individuals
T972	4128-4135	UBERON:0002294	denotes	biliary
T973	4136-4142	CHEBI:15889	denotes	sterol
T974	4143-4152	GO_EXT:secretion_entity_or_process	denotes	secretion
T975	4257-4263	CHEBI:15889	denotes	sterol
T976	4257-4263	_FRAGMENT	denotes	sterol
T977	4265-4276	GO_EXT:0015248	denotes	transporter
T978	4265-4276	GO_EXT:biological_mover_or_transporter	denotes	transporter
T979	4278-4287	GO:0010467	denotes	expressed
T980	4295-4318	UBERON:0012427	denotes	intestinal brush border
T981	4330-4349	UBERON:0001283	denotes	hepatic canalicular
T982	4368-4375	SO_EXT:0000704	denotes	Genetic
T983	4454-4459	NCBITaxon:9606	denotes	human
T984	4460-4470	GO_SO_EXT:chromosome	denotes	chromosome
T985	4534-4541	SO_EXT:sequence_cloning_process	denotes	cloning
T986	4573-4578	SO_EXT:0000704	denotes	genes
T987	4602-4605	PR_EXT:liver_X_receptor	denotes	LXR
T988	4602-4614	CHEBI_EXT:86029	denotes	LXR agonists
T989	4606-4614	CHEBI_GO_EXT:agonist	denotes	agonists
T990	4654-4659	SO_EXT:0000704	denotes	genes
T991	4661-4666	PR_EXT:000003572	denotes	ABCG5
T992	4671-4676	PR_EXT:000003573	denotes	ABCG8
T993	4684-4690	SO_EXT:sequence_coding_function	denotes	encode
T994	4695-4703	CHEBI_PR_EXT:protein	denotes	proteins
T995	4704-4714	PR_EXT:000003572	denotes	sterolin-1
T996	4719-4729	PR_EXT:000003573	denotes	sterolin-2
T997	4731-4740	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T998	4804-4812	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T999	4823-4828	PR_EXT:000003572	denotes	ABCG5
T1000	4838-4843	PR_EXT:000003573	denotes	ABCG8
T1001	4980-4989	SO_EXT:sequence_alteration_entity_or_process	denotes	mutations
T1002	4998-5003	SO_EXT:0000704	denotes	genes
T1003	5067-5078	CHEBI_EXT:biochemical	denotes	biochemical
T1004	5090-5100	PR_EXT:000003572	denotes	sterolin-1
T1005	5105-5115	PR_EXT:000003573	denotes	sterolin-2
T1006	5163-5169	GO_EXT:dimer_macromolecular_complex	denotes	dimers
T1007	5225-5233	CHEBI_PR_EXT:protein	denotes	proteins
T1008	5245-5256	GO_EXT:biological_mover_or_transporter	denotes	transporter
T1009	5279-5287	GO:0016020	denotes	membrane
T1010	5288-5295	SO_EXT:0000417	denotes	domains
T1011	5322-5335	SO_EXT:0001077	denotes	transmembrane
T1012	5327-5335	GO:0016020	denotes	membrane
T1013	5369-5381	GO_EXT:biological_mover_or_transporter	denotes	transporters
T1014	5415-5423	CHEBI_PR_EXT:protein	denotes	proteins
T1015	5485-5495	GO:0010467	denotes	expression
T1016	5499-5504	PR_EXT:000003572	denotes	ABCG5
T1017	5505-5510	PR_EXT:000003573	denotes	ABCG8
T1018	5516-5526	SO_EXT:transgenic_entity	denotes	transgenic
T1019	5527-5532	NCBITaxon:10088	denotes	mouse
T1020	5563-5570	UBERON:0002294	denotes	biliary
T1021	5571-5582	CHEBI:16113	denotes	cholesterol
T1022	5657-5668	CHEBI:16113	denotes	cholesterol
T1023	5702-5707	PR_EXT:000003572	denotes	ABCG5
T1024	5712-5717	PR_EXT:000003573	denotes	ABCG8
T1025	5752-5762	GO_PATO_EXT:biological_maturation	denotes	maturation
T1026	5775-5781	GO:0045177	denotes	apical
T1027	5782-5791	GO_EXT:biological_routing_or_transport	denotes	targeting
T1028	5796-5806	GO:0010467	denotes	expression
T1029	5832-5838	GO:0045177	denotes	apical
T1030	5839-5850	GO_EXT:biological_routing_or_transport	denotes	trafficking
T1031	5866-5870	CL_GO_EXT:cell	denotes	cell
T1032	5899-5904	PR_EXT:000003572	denotes	Abcg5
T1033	5905-5910	PR_EXT:000003573	denotes	Abcg8
T1034	5927-5931	NCBITaxon:10088	denotes	mice
T1035	6036-6048	GO_EXT:biological_mover_or_transporter	denotes	transporters
T1036	6076-6082	GO_EXT:dimer_macromolecular_complex	denotes	dimers
T1037	6117-6124	CHEBI:15889	denotes	sterols
T1038	6140-6148	CL_GO_EXT:cell	denotes	cellular
T1039	6149-6161	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T1040	6175-6183	CHEBI_PR_EXT:protein	denotes	proteins
T1041	6235-6240	PR_EXT:000003572	denotes	Abcg5
T1042	6244-6249	PR_EXT:000003573	denotes	Abcg8
T1043	6259-6264	NCBITaxon:10088	denotes	mouse
T1044	6287-6292	PR_EXT:000003572	denotes	Abcg5
T1045	6297-6302	PR_EXT:000003573	denotes	Abcg8
T1046	6364-6369	NCBITaxon:9606	denotes	human
T1047	6431-6436	NCBITaxon:9606	denotes	human
T1048	6467-6475	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T1049	6496-6500	SO_EXT:0000704	denotes	gene
T1050	6587-6599	GO_EXT:biological_mover_or_transporter	denotes	transporters
T1051	6660-6668	PR_EXT:sterolin	denotes	sterolin
T1052	6707-6714	UBERON:0002294	denotes	biliary
T1053	6715-6724	GO_EXT:secretion_entity_or_process	denotes	secretion
T1054	6728-6735	CHEBI:15889	denotes	sterols
T1055	6739-6749	UBERON:0000160	denotes	intestinal
T1056	6739-6760	GO:0050892	denotes	intestinal absorption
T1057	6764-6771	CHEBI:15889	denotes	sterols
T1058	6812-6818	NCBITaxon:9606	denotes	humans
T1059	6834-6840	UBERON:0000062	denotes	organs
T1060	6916-6921	NCBITaxon:10088	denotes	mouse
T1061	6949-6956	SO_EXT:0000704	denotes	genetic
T1062	6957-6965	SO_EXT:sequence_alteration_entity_or_process	denotes	mutation
T1063	6969-6974	PR_EXT:000003573	denotes	Abcg8
T1064	6976-6980	NCBITaxon:10088	denotes	Mice
T1065	6996-7001	PR_EXT:000003573	denotes	Abcg8
T1066	7050-7055	NCBITaxon:9606	denotes	human
T1067	7114-7121	UBERON:0002294	denotes	biliary
T1068	7122-7128	CHEBI:15889	denotes	sterol
T1069	7129-7138	GO_EXT:secretion_entity_or_process	denotes	secretion
T1070	7169-7178	GO_EXT:secretion_entity_or_process	denotes	secretion
T1071	7180-7185	PR_EXT:000003573	denotes	Abcg8
T1072	7196-7203	NCBITaxon:33208	denotes	animals
T1073	7211-7218	GO:0046903	denotes	secrete
T1074	7219-7230	CHEBI:16113	denotes	cholesterol
T1075	7236-7240	UBERON:0001970	denotes	bile
T1076	7251-7261	CHEBI:27693	denotes	sitosterol
T1077	7262-7271	GO_EXT:secretion_entity_or_process	denotes	secretion
T2126	7301-7317	SO_EXT:0001644	denotes	Targeting vector
T2127	7358-7362	NCBITaxon:10088	denotes	mice
T2128	7365-7370	NCBITaxon:10088	denotes	mouse
T2129	7371-7378	SO_EXT:0001026	denotes	genomic
T2130	7379-7388	NCBITaxon:2	denotes	bacterial
T2131	7379-7410	SO_EXT:0000153	denotes	bacterial artificial chromosome
T2132	7400-7410	GO_SO_EXT:chromosome	denotes	chromosome
T2133	7412-7415	SO_EXT:0000153	denotes	BAC
T2134	7435-7442	CL:0002322	denotes	ES cell
T2135	7438-7442	CL_GO_EXT:cell	denotes	cell
T2136	7464-7472	OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process	denotes	Genetics
T2137	7523-7530	SO_EXT:0000112	denotes	primers
T2138	7549-7558	SO_EXT:biological_sequence	denotes	sequences
T2139	7562-7567	NCBITaxon:10088	denotes	mouse
T2140	7568-7573	PR_EXT:000003572	denotes	Abcg5
T2141	7578-7583	PR_EXT:000003573	denotes	Abcg8
T2142	7584-7588	SO_EXT:cDNA	denotes	cDNA
T2143	7629-7632	SO_EXT:0000153	denotes	BAC
T2144	7633-7638	SO_EXT:sequence_cloned_entity	denotes	clone
T2145	7673-7684	SO_EXT:genomic_DNA	denotes	genomic DNA
T2146	7681-7684	CHEBI_SO_EXT:DNA	denotes	DNA
T2147	7698-7703	PR_EXT:000003573	denotes	Abcg8
T2148	7719-7729	GO_EXT:polymerase	denotes	polymerase
T2149	7879-7880	CHEBI_SO_EXT:base	denotes	b
T2150	7892-7899	SO_EXT:0001026	denotes	genomic
T2151	7928-7932	SO_EXT:0000147	denotes	exon
T2152	7946-7950	SO_EXT:0000147	denotes	exon
T2153	7957-7965	SO_EXT:sequence_insertion_process	denotes	inserted
T2154	7993-8000	SO_EXT:sequence_cloning_process	denotes	cloning
T2155	8020-8026	SO_EXT:0000440	denotes	vector
T2156	8044-8051	SO_EXT:0001026	denotes	genomic
T2157	8081-8085	SO_EXT:0000147	denotes	exon
T2158	8099-8103	SO_EXT:0000147	denotes	exon
T2159	8111-8117	SO_EXT:sequence_cloning_process	denotes	cloned
T2160	8142-8149	SO_EXT:sequence_cloning_process	denotes	cloning
T2161	8204-8210	SO_EXT:0000188	denotes	intron
T2162	8222-8226	SO_EXT:0000147	denotes	exon
T2163	8241-8245	SO_EXT:0000147	denotes	exon
T2164	8248-8259	SO_EXT:sequence_substitution_process	denotes	replacement
T2165	8267-8275	CHEBI:7507	denotes	neomycin
T2166	8287-8295	SO_EXT:0005853	denotes	cassette
T2167	8345-8350	SO_EXT:sequence_or_structure_motif	denotes	motif
T2168	8364-8372	CL:0002322	denotes	ES cells
T2169	8367-8372	CL_GO_EXT:cell	denotes	cells
T2170	8386-8390	CL_GO_EXT:cell	denotes	cell
T2171	8426-8436	SO_EXT:sequence_linearization	denotes	linearized
T2172	8437-8453	SO_EXT:0001644	denotes	targeting vector
T2173	8454-8457	CHEBI_SO_EXT:DNA	denotes	DNA
T2174	8488-8497	UBERON:0000922	denotes	embryonic
T2175	8498-8509	CL:0000057	denotes	fibroblasts
T2176	8511-8522	GO:0009294	denotes	Transfected
T2177	8523-8527	CL_GO_EXT:cell	denotes	cell
T2178	8564-8569	CL_GO_EXT:cell	denotes	cells
T2179	8601-8605	CHEBI:42768	denotes	G418
T2180	8651-8659	CL:0002322	denotes	ES cells
T2181	8654-8659	CL_GO_EXT:cell	denotes	cells
T2182	8689-8702	GO_SO_EXT:sequence_rearrangement_process	denotes	recombination
T2183	8719-8733	SO_EXT:0000121	denotes	forward primer
T2184	8774-8777	CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase	denotes	neo
T2185	8778-8786	SO_EXT:0005853	denotes	cassette
T2186	8792-8806	SO_EXT:0000132	denotes	reverse primer
T2187	8807-8813	SO_EXT:0000188	denotes	intron
T2188	8886-8895	SO_EXT:engineered_biological_sequence	denotes	construct
T2189	8899-8904	NCBITaxon:10088	denotes	mouse
T2190	8905-8910	PR_EXT:000003573	denotes	Abcg8
T2191	8911-8917	SO_EXT:0000188	denotes	intron
T2192	8941-8948	CL:0002322	denotes	ES cell
T2193	8944-8948	CL_GO_EXT:cell	denotes	cell
T2194	8949-8955	SO_EXT:sequence_cloned_entity	denotes	clones
T2195	8999-9006	CL:0002322	denotes	ES cell
T2196	9002-9006	CL_GO_EXT:cell	denotes	cell
T2197	9007-9010	CHEBI_SO_EXT:DNA	denotes	DNA
T2198	9023-9028	PR_EXT:P23940	denotes	BamHI
T2199	9035-9040	CHEBI_SO_EXT:molecular_probe	denotes	probe
T2200	9061-9063	SO_EXT:0000028	denotes	bp
T2201	9090-9096	SO_EXT:0000188	denotes	intron
T2202	9103-9107	SO_EXT:0000147	denotes	exon
T2203	9113-9118	PR_EXT:000003573	denotes	Abcg8
T2204	9119-9123	SO_EXT:0000704	denotes	gene
T2205	9145-9153	CL:0002322	denotes	ES cells
T2206	9148-9153	CL_GO_EXT:cell	denotes	cells
T2207	9185-9196	UBERON:0000358	denotes	blastocysts
T2208	9267-9271	NCBITaxon:10088	denotes	mice
T2209	9280-9284	UBERON:0010166	denotes	coat
T2210	9298-9303	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T2211	9312-9319	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T2212	9339-9343	GO_EXT:breeding	denotes	bred
T2213	9358-9362	NCBITaxon:10088	denotes	mice
T2214	9411-9415	NCBITaxon:10088	denotes	mice
T2215	9419-9424	PR_EXT:000003573	denotes	Abcg8
T2216	9425-9429	SO_EXT:0000704	denotes	gene
T2217	9465-9469	NCBITaxon:10088	denotes	mice
T2218	9500-9504	NCBITaxon:10088	denotes	mice
T2219	9578-9582	NCBITaxon:10088	denotes	mice
T2701	9585-9592	NCBITaxon:33208	denotes	Animals
T2702	9607-9611	NCBITaxon:10088	denotes	mice
T2703	9642-9648	NCBITaxon:9989	denotes	rodent
T2704	9649-9653	CHEBI_EXT:33290	denotes	chow
T2705	9669-9674	NCBITaxon:10088	denotes	mouse
T2706	9675-9678	NCBITaxon:10114	denotes	rat
T2707	9717-9728	CHEBI:16113	denotes	cholesterol
T2708	9739-9750	CHEBI:28623	denotes	campesterol
T2709	9766-9776	CHEBI:27693	denotes	sitosterol
T2710	9799-9804	CHEBI:15377	denotes	water
T2711	9868-9875	NCBITaxon:33208	denotes	animals
T2712	9921-9928	NCBITaxon:33208	denotes	animals
T2713	9968-9974	NCBITaxon:33208	denotes	Animal
T2714	10010-10016	NCBITaxon:33208	denotes	Animal
T2879	10139-10144	NCBITaxon:10088	denotes	Mouse
T2880	10145-10149	UBERON:0002415	denotes	tail
T2881	10150-10153	CHEBI_SO_EXT:DNA	denotes	DNA
T2882	10215-10220	NCBITaxon:10088	denotes	mouse
T2883	10221-10225	UBERON:0002415	denotes	tail
T2884	10253-10258	GO:0019835	denotes	lysis
T2885	10259-10265	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T2886	10273-10277	CHEBI:9754	denotes	Tris
T2887	10278-10281	CHEBI:17883	denotes	HCl
T2888	10297-10301	CHEBI_EXT:EDTA	denotes	EDTA
T2889	10310-10314	CHEBI:26710	denotes	NaCl
T2890	10319-10322	CHEBI:8984	denotes	SDS
T2891	10331-10341	GO_EXT:0008233	denotes	Proteinase
T2892	10394-10398	CHEBI:26710	denotes	NaCl
T2893	10423-10431	CHEBI:75958	denotes	solution
T2894	10485-10493	CHEBI:75958	denotes	solution
T2895	10581-10592	GO_EXT:biological_movement_or_translocation_process	denotes	transferred
T2896	10630-10634	CHEBI:16236	denotes	EtOH
T2897	10663-10666	CHEBI_SO_EXT:DNA	denotes	DNA
T2898	10692-10696	CHEBI:16236	denotes	EtOH
T2899	10730-10736	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T2900	10832-10843	SO_EXT:genomic_DNA	denotes	genomic DNA
T2901	10840-10843	CHEBI_SO_EXT:DNA	denotes	DNA
T2902	10917-10924	SO_EXT:0000112	denotes	primers
T2903	10930-10935	PR_EXT:000003573	denotes	Abcg8
T2904	10936-10940	SO_EXT:0000704	denotes	gene
T2905	10950-10957	SO_EXT:0000112	denotes	primers
T2906	11061-11066	PR_EXT:000003573	denotes	Abcg8
T2907	11120-11126	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T2908	11135-11140	CHEBI:6636	denotes	MgCl2
T2909	11148-11157	CHEBI:62946	denotes	(NH4)2SO4
T2910	11166-11171	CHEBI_EXT:dNTP	denotes	dNTPs
T2911	11179-11183	CHEBI:9754	denotes	Tris
T2912	11184-11187	CHEBI:17883	denotes	HCl
T2913	11210-11216	SO_EXT:0000112	denotes	primer
T2914	11218-11223	CHEBI:15377	denotes	water
T2915	11241-11244	NCBITaxon:271	denotes	Taq
T2916	11245-11255	GO_EXT:polymerase	denotes	polymerase
T2917	11363-11375	SO_EXT:0000006	denotes	PCR products
T3226	11460-11464	CHEBI_SO_EXT:DNA	denotes	DNAs
T3227	11484-11490	PR_EXT:P23940	denotes	Bam HI
T3228	11531-11538	CHEBI:2511	denotes	agarose
T3229	11547-11558	GO_EXT:biological_movement_or_translocation_process	denotes	transferred
T3230	11562-11567	CHEBI:25614	denotes	nylon
T3231	11605-11615	GO:0097617	denotes	hybridized
T3232	11627-11629	SO_EXT:0000028	denotes	bp
T3233	11656-11662	SO_EXT:0000188	denotes	intron
T3234	11669-11673	SO_EXT:0000147	denotes	exon
T3235	11679-11684	PR_EXT:000003573	denotes	Abcg8
T3236	11685-11688	CHEBI:37972	denotes	32P
T3237	11698-11705	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T3238	11706-11711	CHEBI_SO_EXT:molecular_probe	denotes	probe
T3323	11779-11784	UBERON:0002107	denotes	liver
T3324	11789-11799	UBERON:0000160	denotes	intestinal
T3325	11806-11809	CHEBI_SO_EXT:RNA	denotes	RNA
T3326	11815-11820	PR_EXT:000003573	denotes	Abcg8
T3327	11820-11821	SO_EXT:normal_or_wild_type_or_present	denotes	+
T3328	11822-11823	SO_EXT:normal_or_wild_type_or_present	denotes	+
T3329	11825-11830	PR_EXT:000003573	denotes	Abcg8
T3330	11830-11831	SO_EXT:normal_or_wild_type_or_present	denotes	+
T3331	11832-11833	SO_EXT:sequence_nullness_or_absence	denotes	-
T3332	11838-11843	PR_EXT:000003573	denotes	Abcg8
T3333	11843-11844	SO_EXT:sequence_nullness_or_absence	denotes	-
T3334	11845-11846	SO_EXT:sequence_nullness_or_absence	denotes	-
T3335	11847-11851	NCBITaxon:10088	denotes	mice
T3336	11928-11933	CHEBI_SO_EXT:molecular_probe	denotes	probe
T3337	11938-11943	PR_EXT:000003572	denotes	Abcg5
T3338	11953-11955	SO_EXT:0000028	denotes	bp
T3339	11957-11959	CHEBI_SO_EXT:nucleotide	denotes	nt
T3340	12012-12017	CHEBI_SO_EXT:molecular_probe	denotes	probe
T3341	12022-12027	PR_EXT:000003573	denotes	Abcg8
T3342	12037-12039	SO_EXT:0000028	denotes	bp
T3343	12041-12043	CHEBI:25613	denotes	nt
T3443	12090-12111	GO:0001171	denotes	Reverse Transcription
T3444	12113-12115	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3445	12150-12170	GO:0000380	denotes	alternative splicing
T3446	12188-12194	SO_EXT:0001023	denotes	allele
T3447	12196-12198	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3448	12220-12225	SO_EXT:cDNA	denotes	cDNAs
T3449	12236-12241	NCBITaxon:10088	denotes	mouse
T3450	12242-12247	UBERON:0002107	denotes	liver
T3451	12275-12278	CHEBI_SO_EXT:RNA	denotes	RNA
T3452	12300-12305	NCBITaxon:10088	denotes	mouse
T3453	12306-12312	UBERON:0002107	denotes	livers
T3454	12326-12345	GO:0001171	denotes	reverse transcribed
T3455	12451-12459	CHEBI_SO_EXT:hexanucleotide	denotes	hexamers
T3456	12503-12505	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3457	12530-12537	SO_EXT:0000112	denotes	primers
T3458	12769-12771	SO_EXT:0000028	denotes	bp
T3459	12788-12797	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T3460	12798-12802	SO_EXT:cDNA	denotes	cDNA
T3648	12818-12820	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3649	12825-12832	SO_EXT:0000112	denotes	Primers
T3650	12870-12876	SO_EXT:0000112	denotes	primer
T3651	12951-12955	CHEBI_SO_EXT:mRNA	denotes	mRNA
T3652	12959-12963	SO_EXT:cDNA	denotes	cDNA
T3653	12977-12993	_FRAGMENT	denotes	amplification of
T3654	13028-13031	GO:0006277	denotes	DNA
T3655	13020-13031	SO_EXT:genomic_DNA	denotes	genomic DNA
T3656	13028-13031	CHEBI_SO_EXT:DNA	denotes	DNA
T3657	13045-13048	CHEBI_SO_EXT:RNA	denotes	RNA
T3658	13084-13087	CHEBI_SO_EXT:RNA	denotes	RNA
T3659	13109-13114	NCBITaxon:10088	denotes	mouse
T3660	13115-13121	UBERON:0002107	denotes	livers
T3661	13135-13154	GO:0001171	denotes	reverse transcribed
T3662	13260-13268	CHEBI_SO_EXT:hexanucleotide	denotes	hexamers
T3663	13327-13329	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3664	13381-13389	SO_EXT:biological_sequence	denotes	sequence
T3665	13492-13502	CHEBI:51461	denotes	SYBR Green
T3666	13514-13517	CHEBI:60004	denotes	mix
T3667	13564-13567	GO_PR_EXT:uracil_DNA_glycosylase	denotes	UNG
T3668	13568-13574	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T3669	13624-13628	SO_EXT:cDNA	denotes	cDNA
T3670	13643-13664	GO:0001171	denotes	reverse transcription
T3671	13701-13716	CHEBI_SO_EXT:oligonucleotide	denotes	oligonucleotide
T3672	13717-13724	SO_EXT:0000112	denotes	primers
T3673	13743-13745	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3674	13779-13782	GO_PR_EXT:uracil_DNA_glycosylase	denotes	UNG
T3675	13874-13878	GO:0032508	denotes	melt
T3676	13911-13920	GO:0097617	denotes	annealing
T3677	14128-14140	SO_EXT:0000006	denotes	PCR products
T3678	14316-14323	SO_EXT:0000673	denotes	message
T3679	14316-14323	CHEBI:33695	denotes	message
T3680	14327-14332	SO_EXT:0000704	denotes	genes
T3681	14354-14359	NCBITaxon:10088	denotes	mouse
T3682	14360-14365	UBERON:0002107	denotes	liver
T3683	14392-14394	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T3684	14468-14471	CHEBI_SO_EXT:RNA	denotes	RNA
T3685	14678-14682	SO_EXT:0000704	denotes	gene
T3686	14935-14939	CHEBI_SO_EXT:mRNA	denotes	mRNA
T3687	14969-14978	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T3688	15088-15097	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T4195	15101-15107	UBERON:0001969	denotes	Plasma
T4196	15108-15113	CHEBI:18059	denotes	lipid
T4197	15131-15138	CHEBI_PR_EXT:protein	denotes	protein
T4198	15190-15195	UBERON:0000178	denotes	blood
T4199	15229-15234	PR_EXT:000003573	denotes	Abcg8
T4200	15234-15235	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4201	15236-15237	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4202	15239-15244	PR_EXT:000003573	denotes	Abcg8
T4203	15244-15245	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4204	15246-15247	SO_EXT:sequence_nullness_or_absence	denotes	-
T4205	15252-15257	PR_EXT:000003573	denotes	Abcg8
T4206	15257-15258	SO_EXT:sequence_nullness_or_absence	denotes	-
T4207	15259-15260	SO_EXT:sequence_nullness_or_absence	denotes	-
T4208	15261-15265	NCBITaxon:10088	denotes	mice
T4209	15296-15303	UBERON:0001697	denotes	orbital
T4210	15304-15317	UBERON:0001593	denotes	venous plexus
T4211	15358-15368	CHEBI:6015	denotes	isoflurane
T4212	15369-15379	CHEBI_EXT:anaesthetic_process_or_quality	denotes	anesthesia
T4213	15439-15443	CHEBI_EXT:EDTA	denotes	EDTA
T4214	15462-15468	UBERON:0001969	denotes	plasma
T4215	15479-15487	SO_EXT:genotype_or_entity_with_genotype	denotes	genotype
T4216	15571-15578	CHEBI_PR_EXT:protein	denotes	protein
T4411	15721-15727	CHEBI:15889	denotes	Sterol
T4412	15743-15749	UBERON:0001969	denotes	plasma
T4413	15754-15761	UBERON:0000479	denotes	tissues
T4414	15804-15808	NCBITaxon:10088	denotes	mice
T4415	15809-15812	GO:0007631	denotes	fed
T4416	15824-15830	NCBITaxon:9989	denotes	rodent
T4417	15831-15835	CHEBI_EXT:33290	denotes	chow
T4418	15842-15847	UBERON:0000178	denotes	blood
T4419	15852-15859	UBERON:0000479	denotes	tissues
T4420	15881-15892	CHEBI:6015	denotes	isofluorane
T4421	15893-15903	CHEBI_EXT:anaesthetic_process_or_quality	denotes	anesthesia
T4422	15921-15926	PR_EXT:000003573	denotes	Abcg8
T4423	15926-15927	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4424	15928-15929	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4425	15931-15936	PR_EXT:000003573	denotes	Abcg8
T4426	15936-15937	SO_EXT:normal_or_wild_type_or_present	denotes	+
T4427	15938-15939	SO_EXT:sequence_nullness_or_absence	denotes	-
T4428	15944-15949	PR_EXT:000003573	denotes	Abcg8
T4429	15949-15950	SO_EXT:sequence_nullness_or_absence	denotes	-
T4430	15951-15952	SO_EXT:sequence_nullness_or_absence	denotes	-
T4431	15953-15957	NCBITaxon:10088	denotes	mice
T4432	15982-15987	UBERON:0000178	denotes	Blood
T4433	16017-16024	UBERON:0001697	denotes	orbital
T4434	16025-16038	UBERON:0001593	denotes	venous plexus
T4435	16070-16077	NCBITaxon:33208	denotes	animals
T4436	16083-16093	GO_EXT:killing	denotes	sacrificed
T4437	16097-16105	UBERON:0005434	denotes	cervical
T4438	16122-16128	UBERON:0000479	denotes	tissue
T4439	16141-16147	UBERON:0001969	denotes	Plasma
T4440	16179-16183	CHEBI:32145	denotes	NaOH
T4441	16205-16210	CHEBI:15377	denotes	water
T4442	16221-16228	CHEBI:15889	denotes	sterols
T4443	16280-16293	CHEBI:27750	denotes	ethyl acetate
T4444	16312-16325	CHEBI:35515	denotes	5α-cholestane
T4445	16369-16374	NCBITaxon:10088	denotes	Mouse
T4446	16375-16382	UBERON:0000479	denotes	tissues
T4447	16384-16389	UBERON:0002107	denotes	liver
T4448	16391-16397	UBERON:0002106	denotes	spleen
T4449	16402-16407	UBERON:0000955	denotes	brain
T4450	16443-16452	CHEBI_EXT:phosphate	denotes	phosphate
T4451	16453-16459	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T4452	16460-16466	CHEBI_EXT:saline_solution	denotes	saline
T4453	16579-16586	CHEBI_PR_EXT:protein	denotes	protein
T4454	16615-16621	CHEBI:15889	denotes	sterol
T4455	16642-16648	UBERON:0000479	denotes	tissue
T4456	16702-16708	CHEBI:15889	denotes	sterol
T4457	16780-16786	CHEBI:29021	denotes	hexane
T4458	17049-17055	UBERON:0001969	denotes	plasma
T4459	17060-17066	UBERON:0000479	denotes	tissue
T4460	17067-17074	CHEBI:15889	denotes	sterols
T4461	17118-17124	UBERON:0000479	denotes	tissue
T4773	17152-17160	GO:0042571	denotes	antibody
T4774	17152-17171	GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production	denotes	antibody production
T4775	17203-17208	PR_EXT:000003572	denotes	Abcg5
T4776	17209-17219	PR_EXT:000003572	denotes	sterolin-1
T4777	17220-17230	GO:0042571	denotes	antibodies
T4778	17305-17310	PR_EXT:000003572	denotes	Abcg5
T4779	17311-17321	PR_EXT:000003572	denotes	sterolin-1
T4780	17322-17332	GO:0042571	denotes	antibodies
T4781	17410-17415	UBERON:0002107	denotes	Liver
T4886	17467-17475	GO:0016020	denotes	Membrane
T4887	17476-17483	CHEBI_PR_EXT:protein	denotes	protein
T4888	17503-17512	GO:0016020	denotes	membranes
T4889	17561-17566	UBERON:0002107	denotes	liver
T4890	17567-17573	UBERON:0000479	denotes	tissue
T4891	17624-17629	GO:0019835	denotes	lysis
T4892	17630-17636	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T4893	17643-17647	CHEBI:9754	denotes	Tris
T4894	17662-17669	CHEBI:17992	denotes	sucrose
T4895	17675-17694	CHEBI_GO_EXT:peptidase_or_protease_or_proteinase_inhibitor	denotes	protease inhibitors
T4896	18236-18241	GO:0019835	denotes	lysis
T4897	18242-18248	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T4898	18250-18266	GO:0005886	denotes	Plasma membranes
T4899	18311-18318	CHEBI_PR_EXT:protein	denotes	Protein
T4900	18373-18380	CHEBI_PR_EXT:protein	denotes	protein
T4901	18465-18473	GO:0016020	denotes	membrane
T4902	18474-18482	CHEBI_PR_EXT:protein	denotes	proteins
T4903	18510-18513	CHEBI:8984	denotes	SDS
T4904	18514-18524	CHEBI:28619	denotes	acrylamide
T5142	18546-18554	CHEBI_PR_EXT:protein	denotes	Proteins
T5143	18567-18570	CHEBI:8984	denotes	SDS
T5144	18581-18592	GO_EXT:biological_movement_or_translocation_process	denotes	transferred
T5145	18596-18610	CHEBI:53325	denotes	nitrocellulose
T5146	18688-18697	CHEBI_EXT:phosphate	denotes	Phosphate
T5147	18698-18706	CHEBI_CHMO_EXT:buffer_process	denotes	Buffered
T5148	18707-18713	CHEBI_EXT:saline_solution	denotes	saline
T5149	18723-18731	CHEBI:53424	denotes	Tween 20
T5150	18770-18778	GO:0042571	denotes	antibody
T5151	18787-18792	PR_EXT:000003572	denotes	Abcg5
T5152	18811-18812	CHEBI:53424	denotes	T
T5153	18892-18893	CHEBI:53424	denotes	T
T5154	18895-18899	CHEBI:9754	denotes	Tris
T5155	18900-18908	CHEBI_CHMO_EXT:buffer_process	denotes	Buffered
T5156	18909-18915	CHEBI_EXT:saline_solution	denotes	Saline
T5157	18921-18929	CHEBI:53424	denotes	Tween-20
T5158	18943-18947	CHEBI:26710	denotes	NaCl
T5159	18978-18982	NCBITaxon:9925	denotes	goat
T5160	18988-18994	NCBITaxon:9986	denotes	rabbit
T5161	18995-19005	MOP:0000779	denotes	conjugated
T5162	19046-19047	CHEBI:53424	denotes	T
T5163	19124-19125	CHEBI:53424	denotes	T
T5164	19138-19142	CHEBI:26710	denotes	NaCl
T5165	19196-19203	CHEBI_EXT:33893	denotes	Reagent
T5389	19353-19358	UBERON:0002107	denotes	liver
T5390	19363-19372	UBERON:0000160	denotes	intestine
T5391	19373-19379	UBERON:0000479	denotes	tissue
T5392	19536-19547	CHEBI:51686	denotes	hematoxylin
T5393	19607-19615	CHEBI:17790	denotes	methanol
T5394	19674-19682	GO:0042571	denotes	antibody
T5395	19728-19735	CHEBI_SO_EXT:glycine	denotes	glycine
T5396	19740-19744	NCBITaxon:9925	denotes	goat
T5397	19745-19750	UBERON:0001977	denotes	serum
T5398	19807-19817	PR_EXT:000003572	denotes	sterolin-1
T5399	19818-19826	GO:0042571	denotes	antibody
T5400	19926-19934	GO:0042571	denotes	antibody
T5401	19936-19940	NCBITaxon:9925	denotes	goat
T5402	19946-19952	NCBITaxon:9986	denotes	rabbit
T5403	19953-19956	GO:0071735	denotes	IgG
T5404	19957-19967	MOP:0000779	denotes	conjugated
T5405	20147-20152	UBERON:0002107	denotes	Liver
T5406	20179-20186	CHEBI:15347	denotes	acetone
T5407	20232-20240	CHEBI:75958	denotes	solution
T5408	20254-20262	CHEBI:53424	denotes	Tween-20
T5409	20266-20270	NCBITaxon:9925	denotes	goat
T5410	20271-20276	UBERON:0001977	denotes	serum
T5411	20321-20331	PR_EXT:000003572	denotes	sterolin-1
T5412	20357-20361	PR_EXT:000003549	denotes	Bsep
T5413	20394-20399	CHEBI:53422	denotes	Tween
T5414	20467-20472	CHEBI:53422	denotes	Tween
T5415	20491-20499	GO:0042571	denotes	antibody
T5416	20501-20505	NCBITaxon:9925	denotes	goat
T5417	20511-20517	NCBITaxon:9986	denotes	rabbit
T5418	20518-20527	CHEBI:52661	denotes	Alexa 488
T5419	20663-20667	CHEBI:51231	denotes	Dapi
T5420	20674-20682	CHEBI:75958	denotes	solution
T5421	20795-20799	CHEBI:51231	denotes	Dapi
T5422	20854-20859	PR_EXT:000003572	denotes	Abcg5
T5423	20864-20868	PR_EXT:000003549	denotes	Bsep
T5860	21003-21015	GO:0009294	denotes	Transfection
T5861	21044-21049	CL_GO_EXT:cell	denotes	cells
T5862	21056-21061	CL_GO_EXT:cell	denotes	cells
T5863	21079-21090	GO:0009294	denotes	transfected
T5864	21101-21106	NCBITaxon:10088	denotes	mouse
T5865	21107-21112	PR_EXT:000003572	denotes	Abcg5
T5866	21122-21127	NCBITaxon:10088	denotes	mouse
T5867	21128-21133	PR_EXT:000003573	denotes	Abcg8
T5868	21134-21144	GO:0010467	denotes	expression
T5869	21145-21155	SO_EXT:engineered_biological_sequence	denotes	constructs
T5870	21183-21190	CHEBI_EXT:33893	denotes	reagent
T5871	21240-21245	CL_GO_EXT:cell	denotes	cells
T5872	21297-21309	GO:0009294	denotes	transfection
T5873	21342-21347	GO:0040007	denotes	grown
T5874	21363-21366	CHEBI:16526	denotes	CO2
T5875	21411-21421	CHEBI:17334	denotes	penicillin
T5876	21433-21445	CHEBI:17076	denotes	streptomycin
T5877	21454-21463	CHEBI_SO_EXT:glutamine	denotes	glutamine
T5878	21479-21491	GO:0009294	denotes	transfection
T5879	21501-21504	CHEBI_SO_EXT:DNA	denotes	DNA
T5880	21537-21544	CHEBI_EXT:33893	denotes	reagent
T5881	21580-21587	CHEBI:60004	denotes	mixture
T5882	21641-21646	CL_GO_EXT:cell	denotes	cells
T5883	21678-21683	CL_GO_EXT:cell	denotes	cells
T5884	21744-21755	GO:0009294	denotes	transfected
T5885	21756-21761	CL_GO_EXT:cell	denotes	cells
T5886	21805-21815	GO:0010467	denotes	expression
T5887	21850-21857	CHEBI:60004	denotes	mixture
T5888	21861-21868	CHEBI:15347	denotes	acetone
T5889	21869-21877	CHEBI:17790	denotes	methanol
T5890	21914-21919	CL_GO_EXT:cell	denotes	cells
T5891	21941-21945	NCBITaxon:9925	denotes	goat
T5892	21946-21951	UBERON:0001977	denotes	serum
T5893	22020-22028	GO:0042571	denotes	antibody
T5894	22034-22039	PR_EXT:000003572	denotes	Abcg5
T5895	22046-22050	NCBITaxon:9925	denotes	goat
T5896	22051-22056	UBERON:0001977	denotes	serum
T5897	22133-22139	NCBITaxon:9986	denotes	rabbit
T5898	22140-22152	GO:0071736	denotes	IgG antibody
T5899	22169-22174	CL_GO_EXT:cell	denotes	Cells
T6208	22260-22267	UBERON:0002294	denotes	biliary
T6209	22268-22273	CHEBI:18059	denotes	lipid
T6210	22298-22302	NCBITaxon:10088	denotes	Mice
T6211	22308-22321	CHEBI_EXT:anaesthetic_process	denotes	anaesthetized
T6212	22330-22340	UBERON:0001179	denotes	peritoneal
T6213	22362-22370	CHEBI:119915	denotes	fentanyl
T6214	22395-22403	CHEBI:49575	denotes	Diazepam
T6215	22409-22416	UBERON:0000916	denotes	abdomen
T6216	22462-22471	UBERON:0002394	denotes	bile duct
T6217	22477-22488	UBERON:0002110	denotes	gallbladder
T6218	22505-22509	UBERON:0001970	denotes	Bile
T6219	22584-22588	UBERON:0001970	denotes	Bile
T6220	22626-22633	UBERON:0002294	denotes	Biliary
T6221	22634-22638	UBERON:0001970	denotes	bile
T6222	22634-22643	CHEBI:22868	denotes	bile salt
T6223	22645-22651	CHEBI:15889	denotes	sterol
T6224	22656-22668	CHEBI:16247	denotes	phospholipid
T6225	22751-22760	GO_EXT:secretion_entity_or_process	denotes	secretion
T6226	22770-22777	UBERON:0002294	denotes	biliary
T6227	22778-22783	CHEBI:18059	denotes	lipid
T6228	22784-22793	GO_EXT:secretion_entity_or_process	denotes	secretion
T6229	22795-22799	UBERON:0001970	denotes	bile
T6230	22850-22854	UBERON:0001970	denotes	bile
T6231	22850-22859	CHEBI:22868	denotes	bile salt
T6232	22909-22917	CHEBI_EXT:chemical_substance_or_polyatomic_entity	denotes	Chemical
T6233	22970-22982	UBERON:0004711	denotes	jugular vein
T6234	23042-23046	UBERON_EXT:body	denotes	body
T6235	23055-23059	UBERON:0001970	denotes	Bile
T6236	23111-23115	UBERON:0001970	denotes	bile
T6237	23111-23120	CHEBI:22868	denotes	bile salt
T6238	23125-23130	CHEBI:18059	denotes	lipid
T6239	23198-23207	UBERON:0002394	denotes	bile duct
T6240	23227-23238	UBERON:0002110	denotes	gallbladder
T6241	23240-23244	UBERON:0001970	denotes	bile
T6242	23279-23283	NCBITaxon:10088	denotes	mice
T6243	23333-23337	NCBITaxon:10088	denotes	mice
T6244	23390-23394	UBERON_EXT:body	denotes	body
T6245	23471-23477	CHEBI:15889	denotes	Sterol
T6246	23490-23494	UBERON:0001970	denotes	bile
T6623	23554-23561	CHEBI:11814	denotes	HMG-CoA
T6624	23566-23577	CHEBI:16113	denotes	cholesterol
T6625	23566-23592	GO_PR_EXT:cholesterol_7_alpha_hydroxylase	denotes	cholesterol 7α-hydroxylase
T6626	23593-23599	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T6627	23609-23614	NCBITaxon:10088	denotes	Mouse
T6628	23615-23620	UBERON:0002107	denotes	liver
T6629	23702-23707	UBERON:0002107	denotes	liver
T6630	23777-23783	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T6631	23792-23799	CHEBI:17992	denotes	sucrose
T6632	23807-23811	CHEBI:9754	denotes	Tris
T6633	23820-23833	CHEBI:64734	denotes	disodium EDTA
T6634	23842-23845	CHEBI:18320	denotes	DTT
T6635	23988-23994	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T6636	24002-24023	CHEBI:32031	denotes	dipotassium phosphate
T6637	24032-24035	CHEBI:32588	denotes	KCl
T6638	24042-24045	CHEBI:18320	denotes	DTT
T6639	24052-24065	CHEBI:64734	denotes	disodium EDTA
T6640	24071-24079	CHEBI:17754	denotes	glycerol
T6641	24101-24108	CHEBI_PR_EXT:protein	denotes	protein
T6642	24113-24120	CHEBI_PR_EXT:protein	denotes	Protein
T6643	24214-24225	CHEBI:16113	denotes	cholesterol
T6644	24214-24240	GO_PR_EXT:cholesterol_7_alpha_hydroxylase	denotes	cholesterol 7α-hydroxylase
T6645	24375-24382	CHEBI:60004	denotes	mixture
T6646	24418-24437	CHEBI_EXT:potassium_phosphate	denotes	potassium phosphate
T6647	24438-24444	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T6648	24453-24459	CHEBI:32031	denotes	K2HPO4
T6649	24468-24472	CHEBI_EXT:EDTA	denotes	EDTA
T6650	24479-24482	CHEBI:18320	denotes	DTT
T6651	24490-24502	CHEBI:17154	denotes	nicotinamide
T6652	24514-24517	CHEBI:36927	denotes	14C
T6653	24519-24530	CHEBI:16113	denotes	cholesterol
T6654	24582-24596	CHEBI:495055	denotes	β-cyclodextrin
T6655	24653-24660	CHEBI_PR_EXT:protein	denotes	protein
T6656	24708-24713	CHEBI_EXT:NADPH	denotes	NADPH
T6657	24720-24725	CHEBI_EXT:NADPH	denotes	NADPH
T6658	24720-24736	GO:0006741	denotes	NADPH-generating
T6659	24752-24757	CHEBI:18009	denotes	NADP+
T6660	24765-24784	CHEBI:14314	denotes	glucose-6-phosphate
T6661	24795-24814	CHEBI:14314	denotes	glucose-6-phosphate
T6662	24795-24828	GO_PR_EXT:glucose_6_phosphate_dehydrogenase	denotes	glucose-6-phosphate dehydrogenase
T6663	24908-24911	CHEBI:32035	denotes	KOH
T6664	24921-24930	MOP:0000370	denotes	butylated
T6665	24931-24945	CHEBI:24751	denotes	hydroxytoluene
T6666	24960-24969	CHEBI:16236	denotes	ethanolic
T6667	24970-24989	CHEBI:32035	denotes	potassium hydroxide
T6668	24992-24994	CHEBI:29238	denotes	3H
T6669	24996-25017	CHEBI:17500	denotes	7α-hydroxycholesterol
T6670	25124-25131	CHEBI:15889	denotes	sterols
T6671	25166-25174	CHEBI:29021	denotes	n-hexane
T6672	25225-25233	CHEBI_EXT:nitrogen	denotes	nitrogen
T6673	25274-25282	CHEBI:29021	denotes	n-hexane
T6674	25283-25293	CHEBI:17824	denotes	2-propanol
T6675	25326-25332	CHEBI:30563	denotes	silica
T6676	25369-25377	CHEBI:29021	denotes	n-hexane
T6677	25378-25388	CHEBI:17824	denotes	2-propanol
T6678	25406-25427	CHEBI:17500	denotes	7α-hydroxycholesterol
T6679	25452-25460	CHEBI:29021	denotes	n-hexane
T6680	25461-25471	CHEBI:17824	denotes	2-propanol
T6681	25519-25525	CHEBI:30563	denotes	silica
T6682	25538-25544	CHEBI:30563	denotes	Silica
T6683	25591-25604	CHEBI:35702	denotes	diethyl ether
T6684	25783-25790	CHEBI:11814	denotes	HMG-CoA
T6685	25783-25800	GO_PR_EXT:hydroxymethylglutaryl_CoA_reductase	denotes	HMG-CoA reductase
T7247	26081-26088	NCBITaxon:33208	denotes	animals
T7381	26153-26158	PR_EXT:000003573	denotes	Abcg8
T7382	26159-26169	PR_EXT:000003573	denotes	sterolin-2
T7383	26180-26184	NCBITaxon:10088	denotes	mice
T7384	26199-26208	SO_EXT:engineered_biological_sequence	denotes	construct
T7385	26267-26275	GO:0008380	denotes	splicing
T7386	26301-26317	SO_EXT:coding_sequence	denotes	coding sequences
T7387	26328-26333	SO_EXT:0000147	denotes	exons
T7388	26349-26356	CL:0002322	denotes	ES cell
T7389	26352-26356	CL_GO_EXT:cell	denotes	cell
T7390	26382-26392	SO_EXT:sequence_linearization	denotes	linearized
T7391	26402-26409	SO_EXT:0000155	denotes	plasmid
T7392	26410-26413	CHEBI_SO_EXT:DNA	denotes	DNA
T7393	26430-26441	GO_SO_EXT:sequence_rearrangement_process	denotes	recombinant
T7394	26442-26444	CL:0002322	denotes	ES
T7395	26445-26451	SO_EXT:sequence_cloned_entity	denotes	clones
T7396	26463-26465	CL:0002322	denotes	ES
T7397	26466-26472	SO_EXT:sequence_cloned_entity	denotes	clones
T7398	26500-26510	UBERON:0000358	denotes	Blastocyst
T7399	26563-26567	NCBITaxon:10088	denotes	mice
T7400	26575-26580	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	males
T7401	26586-26593	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	females
T7402	26600-26604	PATO_UBERON_EXT:male_or_bearer_of_maleness	denotes	male
T7403	26613-26619	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	female
T7404	26640-26642	CL:0002322	denotes	ES
T7405	26643-26648	SO_EXT:sequence_cloned_entity	denotes	clone
T7406	26687-26693	PATO_UBERON_EXT:female_or_bearer_of_femaleness	denotes	female
T7407	26749-26753	GO_EXT:breeding	denotes	bred
T7408	26795-26799	NCBITaxon:10088	denotes	mice
T7409	26851-26859	GO_EXT:breeding	denotes	Breeding
T7410	26873-26880	NCBITaxon:33208	denotes	animals
T7411	26888-26897	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T7412	26936-26940	NCBITaxon:10088	denotes	mice
T7413	27074-27078	NCBITaxon:10088	denotes	mice
T7414	27100-27104	CHEBI_SO_EXT:mRNA	denotes	mRNA
T7415	27109-27114	PR_EXT:000003573	denotes	Abcg8
T7416	27116-27126	GO:0010467	denotes	expression
T7417	27130-27135	PR_EXT:000003572	denotes	Abcg5
T7418	27198-27218	GO:0000380	denotes	alternative splicing
T7419	27228-27241	_FRAGMENT	denotes	production of
T7420	27290-27297	GO:0006412	denotes	protein
T7421	27263-27272	SO_EXT:sequence_truncation_process	denotes	truncated
T7422	27273-27278	PR_EXT:000003573	denotes	Abcg8
T7423	27279-27289	PR_EXT:000003573	denotes	sterolin-2
T7424	27290-27297	CHEBI_PR_EXT:protein	denotes	protein
T7425	27332-27337	PR_EXT:000003572	denotes	Abcg5
T7426	27338-27348	PR_EXT:000003572	denotes	sterolin-1
T7427	27350-27352	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T7428	27374-27378	SO_EXT:cDNA	denotes	cDNA
T7429	27379-27398	GO:0001171	denotes	reverse transcribed
T7430	27410-27415	UBERON:0002107	denotes	liver
T7431	27416-27419	CHEBI_SO_EXT:RNA	denotes	RNA
T7432	27425-27430	PR_EXT:000003573	denotes	Abcg8
T7433	27441-27445	NCBITaxon:10088	denotes	mice
T7434	27450-27454	CHEBI_SO_EXT:mRNA	denotes	mRNA
T7435	27459-27464	PR_EXT:000003573	denotes	Abcg8
T7436	27465-27475	PR_EXT:000003573	denotes	sterolin-2
T7437	27504-27513	SO_EXT:biological_sequence	denotes	sequences
T7438	27514-27524	SO_EXT:sequence_downstreamness	denotes	downstream
T7439	27528-27532	SO_EXT:0000147	denotes	exon
T7440	27542-27547	PR_EXT:000003573	denotes	Abcg8
T7441	27558-27562	NCBITaxon:10088	denotes	mice
T7442	27572-27579	SO_EXT:0000112	denotes	primers
T7443	27596-27601	SO_EXT:0000147	denotes	exons
T7444	27612-27617	SO_EXT:0000147	denotes	exons
T7445	27630-27635	SO_EXT:0000147	denotes	exons
T7446	27691-27712	GO:0000380	denotes	alternatively spliced
T7447	27691-27720	SO_EXT:1001187	denotes	alternatively spliced message
T7448	27713-27720	CHEBI:33695	denotes	message
T7449	27739-27743	SO_EXT:sequence_coding_function	denotes	code
T7450	27750-27759	SO_EXT:sequence_truncation_process	denotes	truncated
T7451	27760-27767	CHEBI_PR_EXT:protein	denotes	protein
T7452	27797-27802	PR_EXT:000003573	denotes	Abcg8
T7453	27813-27817	NCBITaxon:10088	denotes	mice
T7454	27823-27831	GO:0016020	denotes	membrane
T7455	27841-27848	SO_EXT:0000417	denotes	domains
T7456	27852-27857	PR_EXT:000003573	denotes	Abcg8
T7457	27858-27868	PR_EXT:000003573	denotes	sterolin-2
T7458	27873-27880	SO_EXT:sequence_coding_function	denotes	encoded
T7459	27884-27889	SO_EXT:0000147	denotes	exons
T7989	27897-27903	CHEBI:15889	denotes	Sterol
T7990	27914-27919	PR_EXT:000003573	denotes	Abcg8
T7991	27920-27930	PR_EXT:000003573	denotes	sterolin-2
T7992	27941-27948	NCBITaxon:33208	denotes	animals
T7993	27958-27963	NCBITaxon:3193	denotes	plant
T7994	27964-27971	CHEBI:15889	denotes	sterols
T7995	27975-27981	UBERON:0000479	denotes	tissue
T7996	27986-27992	UBERON:0001969	denotes	plasma
T7997	28027-28033	CHEBI:15889	denotes	Sterol
T7998	28096-28102	UBERON:0001969	denotes	plasma
T7999	28107-28114	UBERON:0000479	denotes	tissues
T8000	28122-28127	PR_EXT:000003573	denotes	Abcg8
T8001	28127-28128	SO_EXT:sequence_nullness_or_absence	denotes	-
T8002	28129-28130	SO_EXT:sequence_nullness_or_absence	denotes	-
T8003	28131-28135	NCBITaxon:10088	denotes	mice
T8004	28187-28193	UBERON:0000479	denotes	tissue
T8005	28210-28220	SO_EXT:wild_type_entity_or_quality	denotes	wild types
T8006	28251-28257	UBERON:0001969	denotes	Plasma
T8007	28258-28269	CHEBI:16113	denotes	cholesterol
T8008	28312-28316	NCBITaxon:10088	denotes	mice
T8009	28383-28392	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8010	28393-28397	NCBITaxon:10088	denotes	mice
T8011	28427-28432	NCBITaxon:3193	denotes	Plant
T8012	28433-28440	CHEBI:15889	denotes	sterols
T8013	28469-28478	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8014	28496-28500	NCBITaxon:10088	denotes	mice
T8015	28537-28542	PR_EXT:000003573	denotes	Abcg8
T8016	28542-28543	SO_EXT:sequence_nullness_or_absence	denotes	-
T8017	28544-28545	SO_EXT:sequence_nullness_or_absence	denotes	-
T8018	28546-28550	NCBITaxon:10088	denotes	mice
T8019	28552-28558	UBERON:0001969	denotes	Plasma
T8020	28559-28570	CHEBI:28623	denotes	campesterol
T8021	28575-28585	CHEBI:27693	denotes	sitosterol
T8022	28627-28632	PR_EXT:000003573	denotes	Abcg8
T8023	28632-28633	SO_EXT:sequence_nullness_or_absence	denotes	-
T8024	28634-28635	SO_EXT:sequence_nullness_or_absence	denotes	-
T8025	28636-28640	NCBITaxon:10088	denotes	mice
T8026	28653-28662	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8027	28663-28667	NCBITaxon:10088	denotes	mice
T8028	28805-28811	UBERON:0001969	denotes	plasma
T8029	28812-28817	NCBITaxon:3193	denotes	plant
T8030	28818-28824	CHEBI:15889	denotes	sterol
T8031	28857-28866	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8032	28867-28871	NCBITaxon:10088	denotes	mice
T8033	28873-28878	UBERON:0002107	denotes	Liver
T8034	28879-28890	CHEBI:16113	denotes	cholesterol
T8035	28906-28911	PR_EXT:000003573	denotes	Abcg8
T8036	28911-28912	SO_EXT:sequence_nullness_or_absence	denotes	-
T8037	28913-28914	SO_EXT:sequence_nullness_or_absence	denotes	-
T8038	28915-28919	NCBITaxon:10088	denotes	mice
T8039	28952-28961	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8040	28962-28967	UBERON:0002107	denotes	liver
T8041	28996-29002	UBERON:0000479	denotes	tissue
T8042	29038-29044	UBERON:0000479	denotes	tissue
T8043	29073-29084	CHEBI:16113	denotes	cholesterol
T8044	29131-29142	CHEBI:28623	denotes	campesterol
T8045	29169-29179	CHEBI:27693	denotes	sitosterol
T8046	29227-29233	UBERON:0000479	denotes	tissue
T8047	29252-29257	PR_EXT:000003573	denotes	Abcg8
T8048	29257-29258	SO_EXT:sequence_nullness_or_absence	denotes	-
T8049	29259-29260	SO_EXT:sequence_nullness_or_absence	denotes	-
T8050	29270-29274	NCBITaxon:10088	denotes	mice
T8051	29287-29296	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8052	29297-29301	NCBITaxon:10088	denotes	mice
T8053	29346-29352	UBERON:0000479	denotes	tissue
T8054	29419-29424	UBERON:0002107	denotes	liver
T8055	29425-29436	CHEBI:16113	denotes	cholesterol
T8056	29440-29445	NCBITaxon:3193	denotes	plant
T8057	29446-29452	CHEBI:15889	denotes	sterol
T8058	29470-29475	PR_EXT:000003573	denotes	Abcg8
T8059	29475-29476	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8060	29477-29478	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8061	29483-29488	PR_EXT:000003573	denotes	Abcg8
T8062	29488-29489	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8063	29490-29491	SO_EXT:sequence_nullness_or_absence	denotes	-
T8064	29492-29496	NCBITaxon:10088	denotes	mice
T8065	29498-29504	UBERON:0002106	denotes	Spleen
T8066	29505-29511	CHEBI:15889	denotes	sterol
T8067	29535-29540	UBERON:0002107	denotes	liver
T8068	29591-29596	UBERON:0000955	denotes	brain
T8069	29597-29603	CHEBI:15889	denotes	sterol
T8070	29660-29679	UBERON:0000120	denotes	blood brain barrier
T8071	29703-29711	_FRAGMENT	denotes	entry of
T8072	29718-29725	GO:0035376	denotes	sterols
T8073	29718-29725	CHEBI:15889	denotes	sterols
T8074	29781-29786	NCBITaxon:3193	denotes	plant
T8075	29787-29794	CHEBI:15889	denotes	sterols
T8076	29802-29808	UBERON:0000955	denotes	brains
T8077	29823-29830	NCBITaxon:33208	denotes	animals
T8078	29843-29848	UBERON:0000178	denotes	blood
T8079	29870-29876	UBERON:0000479	denotes	tissue
T8080	29933-29939	UBERON:0000479	denotes	tissue
T8081	29940-29945	NCBITaxon:3193	denotes	plant
T8082	29946-29953	CHEBI:15889	denotes	sterols
T8083	29976-29982	UBERON:0002107	denotes	livers
T8084	29986-29991	PR_EXT:000003573	denotes	Abcg8
T8085	29991-29992	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8086	29993-29994	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8087	29996-29997	SO_EXT:normal_or_wild_type_or_present	denotes	+
T8088	29998-29999	SO_EXT:sequence_nullness_or_absence	denotes	-
T8089	30004-30005	SO_EXT:sequence_nullness_or_absence	denotes	-
T8090	30006-30007	SO_EXT:sequence_nullness_or_absence	denotes	-
T8091	30008-30012	NCBITaxon:10088	denotes	mice
T8092	30047-30069	CHEBI:17002	denotes	esterified cholesterol
T8093	30133-30143	CHEBI:27693	denotes	sitosterol
T8094	30147-30158	CHEBI:28623	denotes	campesterol
T8095	30246-30252	UBERON:0000479	denotes	tissue
T8096	30253-30259	CHEBI:15889	denotes	sterol
T8097	30278-30285	CHEBI:15889	denotes	sterols
T8099	30323-30336	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatically
T8100	30363-30370	CHEBI:15889	denotes	sterols
T8101	30376-30389	CHEBI:17855	denotes	triglycerides
T8102	30408-30414	UBERON:0001969	denotes	plasma
T8103	30435-30442	NCBITaxon:33208	denotes	animals
T8104	30443-30446	GO:0007631	denotes	fed
T8105	30457-30461	CHEBI_EXT:33290	denotes	chow
T8106	30517-30523	CHEBI:15889	denotes	sterol
T8107	30568-30573	PR_EXT:000003573	denotes	Abcg8
T8108	30573-30574	SO_EXT:sequence_nullness_or_absence	denotes	-
T8109	30575-30576	SO_EXT:sequence_nullness_or_absence	denotes	-
T8110	30586-30590	NCBITaxon:10088	denotes	mice
T8111	30618-30630	CHEBI:17855	denotes	triglyceride
T8112	30644-30659	CHEBI_GO_EXT:low_density_lipoprotein_particle	denotes	LDL lipoprotein
T8113	30682-30691	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T8114	30709-30713	NCBITaxon:10088	denotes	mice
T8115	30841-30848	NCBITaxon:33208	denotes	animals
T8116	30856-30862	UBERON:0001969	denotes	plasma
T8117	30863-30875	CHEBI:17855	denotes	triglyceride
T8118	30890-30895	PR_EXT:000003573	denotes	Abcg8
T8119	30895-30896	SO_EXT:sequence_nullness_or_absence	denotes	-
T8120	30897-30898	SO_EXT:sequence_nullness_or_absence	denotes	-
T8121	30899-30903	NCBITaxon:10088	denotes	mice
T8122	30958-30967	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T8123	31033-31042	SO_EXT:genotype_or_entity_with_genotype	denotes	genotypes
T8124	31069-31077	CHEBI_PR_EXT:protein	denotes	proteins
T8125	31149-31164	CHEBI:39015	denotes	apolipoproteins
T8126	31149-31166	PR_EXT:000004145	denotes	apolipoproteins B
T8127	31149-31164	_FRAGMENT	denotes	apolipoproteins
T8128	31168-31169	PR_EXT:000004155	denotes	E
T8129	31171-31173	PR_EXT:000004140	denotes	AI
T8130	31178-31181	PR_EXT:000004142	denotes	AII
T8131	31208-31220	CHEBI:17855	denotes	triglyceride
T8978	31260-31265	UBERON:0002107	denotes	Liver
T8979	31266-31270	SO_EXT:0000704	denotes	gene
T8980	31266-31281	GO:0010467	denotes	gene expression
T8981	31286-31292	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T8982	31312-31317	PR_EXT:000003573	denotes	Abcg8
T8983	31318-31328	PR_EXT:000003573	denotes	sterolin-2
T8984	31339-31343	NCBITaxon:10088	denotes	mice
T8985	31390-31395	PR_EXT:000003573	denotes	Abcg8
T8986	31396-31406	PR_EXT:000003573	denotes	sterolin-2
T8987	31414-31419	SO_EXT:0000704	denotes	genes
T8988	31425-31433	GO:0065007	denotes	regulate
T8989	31434-31440	CHEBI:15889	denotes	sterol
T8990	31434-31451	GO:0016125	denotes	sterol metabolism
T8991	31466-31468	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T8992	31502-31512	GO:0010467	denotes	expression
T8993	31523-31528	PR_EXT:000003572	denotes	Abcg5
T8994	31530-31535	PR_EXT:000003573	denotes	Abcg8
T8995	31537-31541	GO_PR_EXT:hydroxymethylglutaryl_CoA_reductase	denotes	Hmgr
T8996	31543-31549	PR_EXT:000006148	denotes	Cyp7a1
T8997	31551-31556	PR_EXT:000003537	denotes	Abca1
T8998	31558-31562	PR_EXT:P21440	denotes	Mdr2
T8999	31564-31567	PR_EXT:liver_X_receptor	denotes	Lxr
T9000	31569-31574	PR_EXT:000028988	denotes	Srebp
T9001	31583-31590	PR_EXT:000015612	denotes	Srebp-2
T9002	31591-31595	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9003	31603-31609	UBERON:0002107	denotes	livers
T9004	31613-31617	NCBITaxon:10088	denotes	mice
T9005	31618-31621	GO:0007631	denotes	fed
T9006	31632-31636	CHEBI_EXT:33290	denotes	chow
T9007	31668-31673	PR_EXT:000003573	denotes	Abcg8
T9008	31674-31678	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9009	31679-31689	GO:0010467	denotes	expression
T9010	31722-31727	PR_EXT:000003573	denotes	Abcg8
T9011	31727-31728	SO_EXT:sequence_nullness_or_absence	denotes	-
T9012	31729-31730	SO_EXT:sequence_nullness_or_absence	denotes	-
T9013	31731-31735	NCBITaxon:10088	denotes	mice
T9014	31781-31785	NCBITaxon:10088	denotes	mice
T9015	31799-31808	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9016	31809-31813	NCBITaxon:10088	denotes	mice
T9017	31846-31848	GO_EXT:reverse_transcription_or_reverse_transcriptase	denotes	RT
T9018	31858-31862	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9019	31863-31873	GO:0010467	denotes	expression
T9020	31877-31882	PR_EXT:000003572	denotes	Abcg5
T9021	31900-31904	NCBITaxon:10088	denotes	mice
T9022	31955-31964	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9023	31965-31969	NCBITaxon:10088	denotes	mice
T9024	32022-32026	NCBITaxon:10088	denotes	mice
T9025	32028-32038	GO:0010467	denotes	Expression
T9026	32042-32049	CHEBI:11814	denotes	HMG-CoA
T9027	32042-32059	GO_PR_EXT:hydroxymethylglutaryl_CoA_reductase	denotes	HMG-CoA reductase
T9028	32060-32064	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9029	32129-32133	NCBITaxon:10088	denotes	mice
T9030	32188-32193	NCBITaxon:9606	denotes	human
T9031	32249-32253	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9032	32292-32298	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T9033	32317-32322	UBERON:0002107	denotes	liver
T9034	32349-32356	CHEBI:11814	denotes	HMG-CoA
T9035	32349-32366	GO_PR_EXT:hydroxymethylglutaryl_CoA_reductase	denotes	HMG-CoA reductase
T9036	32380-32386	PR_EXT:000006148	denotes	Cyp7a1
T9037	32409-32416	CHEBI:11814	denotes	HMG-CoA
T9038	32409-32426	GO_PR_EXT:hydroxymethylglutaryl_CoA_reductase	denotes	HMG-CoA reductase
T9039	32470-32475	PR_EXT:000003573	denotes	Abcg8
T9040	32475-32476	SO_EXT:normal_or_wild_type_or_present	denotes	+
T9041	32477-32478	SO_EXT:sequence_nullness_or_absence	denotes	-
T9042	32483-32488	PR_EXT:000003573	denotes	Abcg8
T9043	32488-32489	SO_EXT:sequence_nullness_or_absence	denotes	-
T9044	32490-32491	SO_EXT:sequence_nullness_or_absence	denotes	-
T9045	32492-32496	NCBITaxon:10088	denotes	mice
T9046	32558-32562	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9047	32563-32573	GO:0010467	denotes	expression
T9048	32601-32607	PR_EXT:000006148	denotes	Cyp7a1
T9049	32608-32612	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9050	32613-32623	GO:0010467	denotes	expression
T9051	32674-32679	NCBITaxon:10088	denotes	mouse
T9052	32681-32687	PR_EXT:000006148	denotes	Cyp7a1
T9053	32764-32768	NCBITaxon:10088	denotes	mice
T9054	32779-32783	CHEBI_SO_EXT:mRNA	denotes	mRNA
T9055	32800-32806	PR_EXT:000006148	denotes	Cyp7a1
T9056	32823-32833	CHEBI:27693	denotes	Sitosterol
T9057	32870-32879	CHEBI_EXT:35222	denotes	inhibitor
T9058	32883-32889	PR_EXT:000006148	denotes	Cyp7a1
T9059	32925-32930	NCBITaxon:3193	denotes	plant
T9060	32931-32938	CHEBI:15889	denotes	sterols
T9061	32946-32951	UBERON:0002107	denotes	liver
T9062	33003-33008	NCBITaxon:10088	denotes	mouse
T9700	33016-33028	GO_PATO_EXT:biological_localization_process_or_quality	denotes	Localization
T9701	33032-33037	PR_EXT:000003572	denotes	Abcg5
T9702	33038-33045	CHEBI_PR_EXT:protein	denotes	protein
T9703	33049-33054	PR_EXT:000003573	denotes	Abcg8
T9704	33054-33055	SO_EXT:sequence_nullness_or_absence	denotes	-
T9705	33056-33057	SO_EXT:sequence_nullness_or_absence	denotes	-
T9706	33067-33071	NCBITaxon:10088	denotes	mice
T9707	33089-33094	PR_EXT:000003573	denotes	Abcg8
T9708	33095-33105	PR_EXT:000003573	denotes	sterolin-2
T9709	33124-33129	PR_EXT:000003572	denotes	Abcg5
T9710	33130-33140	PR_EXT:000003572	denotes	sterolin-1
T9711	33141-33151	GO:0010467	denotes	expression
T9712	33184-33191	SO_EXT:0000704	denotes	genetic
T9713	33248-33258	GO:0010467	denotes	expression
T9714	33277-33285	GO:0042571	denotes	antibody
T9715	33289-33294	NCBITaxon:10088	denotes	mouse
T9716	33295-33300	PR_EXT:000003573	denotes	Abcg8
T9717	33301-33311	PR_EXT:000003573	denotes	sterolin-2
T9718	33386-33392	NCBITaxon:9986	denotes	rabbit
T9719	33404-33412	GO:0042571	denotes	antibody
T9720	33416-33421	NCBITaxon:10088	denotes	mouse
T9721	33422-33427	PR_EXT:000003572	denotes	Abcg5
T9722	33428-33438	PR_EXT:000003572	denotes	sterolin-1
T9723	33452-33462	GO:0042571	denotes	antibodies
T9724	33537-33542	UBERON:0002107	denotes	liver
T9725	33547-33556	UBERON:0000160	denotes	intestine
T9726	33560-33565	PR_EXT:000003573	denotes	Abcg8
T9727	33565-33566	SO_EXT:sequence_nullness_or_absence	denotes	-
T9728	33567-33568	SO_EXT:sequence_nullness_or_absence	denotes	-
T9729	33569-33573	NCBITaxon:10088	denotes	mice
T9730	33595-33600	UBERON:0002107	denotes	liver
T9731	33607-33615	GO:0016020	denotes	membrane
T9732	33671-33676	PR_EXT:000003572	denotes	Abcg5
T9733	33677-33687	PR_EXT:000003572	denotes	sterolin-1
T9734	33688-33698	GO:0042571	denotes	antibodies
T9735	33759-33764	PR_EXT:000003572	denotes	Abcg5
T9736	33765-33775	PR_EXT:000003572	denotes	sterolin-1
T9737	33828-33835	CHEBI_PR_EXT:protein	denotes	protein
T9738	33848-33860	GO_MOP_EXT:glycosylation	denotes	glycosylated
T9739	33877-33884	CHEBI_PR_EXT:protein	denotes	protein
T9740	33912-33917	PR_EXT:000003572	denotes	Abcg5
T9741	33918-33928	PR_EXT:000003572	denotes	sterolin-1
T9742	33929-33936	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T9743	33937-33945	GO:0042571	denotes	antibody
T9744	33991-33999	GO:0042571	denotes	antibody
T9745	34049-34058	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9746	34071-34078	NCBITaxon:33208	denotes	animals
T9747	34122-34128	PR_EXT:000007078	denotes	Endo-H
T9748	34190-34201	PR_EXT:000016261	denotes	transferrin
T9749	34212-34220	GO:0042571	denotes	antibody
T9750	34250-34255	PR_EXT:000003572	denotes	Abcg5
T9751	34256-34266	PR_EXT:000003572	denotes	sterolin-1
T9752	34270-34279	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9753	34280-34287	NCBITaxon:33208	denotes	animals
T9754	34373-34381	GO:0042571	denotes	antibody
T9755	34407-34413	PR_EXT:000007078	denotes	Endo-H
T9756	34418-34424	PR_EXT:000011196	denotes	PNGase
T9757	34418-34424	GO_EXT:0000224	denotes	PNGase
T9758	34459-34467	GO:0042571	denotes	antibody
T9759	34513-34518	PR_EXT:000003572	denotes	Abcg5
T9760	34519-34529	PR_EXT:000003572	denotes	sterolin-1
T9761	34533-34542	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9762	34543-34550	NCBITaxon:33208	denotes	animals
T9763	34588-34593	NCBITaxon:10088	denotes	mouse
T9764	34635-34645	GO:0042571	denotes	antibodies
T9765	34659-34664	PR_EXT:000003572	denotes	Abcg5
T9766	34665-34675	PR_EXT:000003572	denotes	sterolin-1
T9767	34777-34787	GO:0042571	denotes	antibodies
T9768	34798-34810	GO:0009294	denotes	transfection
T9769	34827-34835	GO:0042571	denotes	antibody
T9770	34852-34861	GO:0010467	denotes	expressed
T9771	34862-34867	NCBITaxon:10088	denotes	mouse
T9772	34868-34873	PR_EXT:000003572	denotes	Abcg5
T9773	34874-34884	PR_EXT:000003572	denotes	sterolin-1
T9774	34894-34899	CL_GO_EXT:cell	denotes	cells
T9775	34909-34914	NCBITaxon:10088	denotes	mouse
T9776	34915-34920	PR_EXT:000003573	denotes	Abcg8
T9777	34921-34931	PR_EXT:000003573	denotes	sterolin-2
T9778	34951-34963	GO:0009294	denotes	transfection
T9779	34973-34978	CL_GO_EXT:cell	denotes	cells
T9780	34989-34994	PR_EXT:000003572	denotes	Abcg5
T9781	34999-35004	PR_EXT:000003573	denotes	Abcg8
T9782	35005-35010	SO_EXT:cDNA	denotes	cDNAs
T9783	35109-35119	GO:0010467	denotes	expression
T9784	35123-35128	PR_EXT:000003573	denotes	Abcg8
T9785	35132-35137	CL_GO_EXT:cell	denotes	cells
T9786	35138-35148	GO:0010467	denotes	expressing
T9787	35149-35154	PR_EXT:000003572	denotes	Abcg5
T9788	35155-35165	PR_EXT:000003572	denotes	sterolin-1
T9789	35190-35198	GO:0042571	denotes	antibody
T9790	35203-35208	PR_EXT:000003573	denotes	Abcg8
T9791	35209-35219	PR_EXT:000003573	denotes	sterolin-2
T9792	35285-35293	GO:0042571	denotes	antibody
T9793	35308-35313	PR_EXT:000003572	denotes	Abcg5
T9794	35314-35324	PR_EXT:000003572	denotes	sterolin-1
T9795	35355-35360	PR_EXT:000003573	denotes	Abcg8
T9796	35361-35371	PR_EXT:000003573	denotes	sterolin-2
T9797	35392-35402	UBERON:0000160	denotes	intestinal
T9798	35403-35409	UBERON:0000479	denotes	tissue
T9799	35435-35440	PR_EXT:000003572	denotes	Abcg5
T9800	35441-35451	PR_EXT:000003572	denotes	sterolin-1
T9801	35469-35477	CL_GO_EXT:cell	denotes	cellular
T9802	35490-35495	PR_EXT:000003572	denotes	Abcg5
T9803	35496-35506	PR_EXT:000003572	denotes	sterolin-1
T9804	35535-35540	PR_EXT:000003572	denotes	Abcg5
T9805	35541-35551	PR_EXT:000003572	denotes	sterolin-1
T9806	35559-35569	UBERON:0000160	denotes	intestinal
T9807	35590-35596	GO:0045177	denotes	apical
T9808	35601-35610	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T9809	35618-35623	GO_UBERON_EXT:villus	denotes	villi
T9810	35631-35642	CL:0000584	denotes	enterocytes
T9811	35670-35675	PR_EXT:000003573	denotes	Abcg8
T9812	35676-35686	PR_EXT:000003573	denotes	sterolin-2
T9813	35765-35773	GO:0042571	denotes	antibody
T9814	35790-35795	PR_EXT:000003572	denotes	Abcg5
T9815	35796-35806	PR_EXT:000003572	denotes	sterolin-1
T9816	35839-35847	GO:0042571	denotes	antibody
T9817	35874-35881	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	peptide
T9818	35942-35947	PR_EXT:000003572	denotes	Abcg5
T9819	35948-35958	PR_EXT:000003572	denotes	sterolin-1
T9820	35959-35969	GO:0010467	denotes	expression
T9821	35994-36000	GO:0045177	denotes	apical
T9822	36064-36072	GO:0042571	denotes	antibody
T9823	36082-36087	UBERON:0002107	denotes	liver
T9824	36102-36111	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9825	36125-36129	NCBITaxon:10088	denotes	mice
T9826	36154-36164	GO:0010467	denotes	expression
T9827	36168-36174	PR_EXT:000003549	denotes	Abcb11
T9828	36183-36194	GO_EXT:biological_mover_or_transporter	denotes	transporter
T9829	36227-36233	GO_RO_EXT:biological_export	denotes	export
T9830	36237-36241	UBERON:0001970	denotes	bile
T9831	36237-36247	CHEBI:22868	denotes	bile salts
T9832	36273-36278	PR_EXT:000003572	denotes	Abcg5
T9833	36279-36289	PR_EXT:000003572	denotes	sterolin-1
T9834	36318-36326	CHEBI_PR_EXT:protein	denotes	proteins
T9835	36331-36339	GO:0045177	denotes	apically
T9836	36340-36349	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localized
T9837	36353-36358	UBERON:0002107	denotes	liver
T9838	36373-36382	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9839	36383-36387	NCBITaxon:10088	denotes	mice
T9840	36435-36440	UBERON:0002107	denotes	liver
T9841	36455-36459	NCBITaxon:10088	denotes	mice
T9842	36474-36484	GO:0010467	denotes	expression
T9843	36494-36499	PR_EXT:000003572	denotes	Abcg5
T9844	36500-36510	PR_EXT:000003572	denotes	sterolin-1
T9845	36588-36594	GO:0045177	denotes	apical
T9846	36595-36605	GO:0010467	denotes	expression
T9847	36609-36614	PR_EXT:000003572	denotes	Abcg5
T9848	36615-36625	PR_EXT:000003572	denotes	sterolin-1
T9849	36642-36646	NCBITaxon:10088	denotes	mice
T9850	36657-36665	GO:0042571	denotes	antibody
T9851	36684-36689	UBERON:0002107	denotes	liver
T9852	36702-36711	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9853	36725-36729	NCBITaxon:10088	denotes	mice
T9854	36747-36753	GO:0045177	denotes	apical
T9855	36754-36764	GO:0010467	denotes	expression
T9856	36768-36773	PR_EXT:000003572	denotes	Abcg5
T9857	36774-36784	PR_EXT:000003572	denotes	sterolin-1
T9858	36793-36802	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T9859	36816-36822	UBERON:0002107	denotes	livers
T10843	36840-36847	UBERON:0002294	denotes	Biliary
T10844	36848-36857	GO_EXT:secretion_entity_or_process	denotes	secretion
T10845	36861-36865	UBERON:0001970	denotes	bile
T10846	36861-36871	CHEBI:22868	denotes	bile salts
T10847	36873-36879	CHEBI:15889	denotes	sterol
T10848	36884-36897	CHEBI:16247	denotes	phospholipids
T10849	36901-36906	PR_EXT:000003573	denotes	Abcg8
T10850	36907-36917	PR_EXT:000003573	denotes	sterolin-2
T10851	36928-36932	NCBITaxon:10088	denotes	mice
T10852	36948-36959	NCBITaxon:1	denotes	individuals
T10853	36988-36995	GO:0046903	denotes	secrete
T10854	36996-37007	CHEBI:16113	denotes	cholesterol
T10855	37012-37017	NCBITaxon:3193	denotes	plant
T10856	37018-37025	CHEBI:15889	denotes	sterols
T10857	37031-37035	UBERON:0001970	denotes	bile
T10858	37070-37074	NCBITaxon:10088	denotes	mice
T10859	37098-37105	UBERON:0002294	denotes	biliary
T10860	37106-37112	CHEBI:15889	denotes	sterol
T10861	37153-37157	UBERON:0001970	denotes	bile
T10862	37158-37167	GO_EXT:secretion_entity_or_process	denotes	secretion
T10863	37184-37190	CHEBI:15889	denotes	sterol
T10864	37195-37207	CHEBI:16247	denotes	phospholipid
T10865	37220-37225	PR_EXT:000003573	denotes	Abcg8
T10866	37225-37226	SO_EXT:sequence_nullness_or_absence	denotes	-
T10867	37227-37228	SO_EXT:sequence_nullness_or_absence	denotes	-
T10868	37229-37233	NCBITaxon:10088	denotes	mice
T10869	37262-37271	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T10870	37272-37276	NCBITaxon:10088	denotes	mice
T10871	37296-37300	UBERON:0001970	denotes	bile
T10872	37296-37305	CHEBI:22868	denotes	bile salt
T10873	37306-37315	GO_EXT:secretion_entity_or_process	denotes	secretion
T10874	37351-37357	CHEBI:15889	denotes	sterol
T10875	37358-37367	GO_EXT:secretion_entity_or_process	denotes	secretion
T10876	37385-37397	CHEBI:16247	denotes	phospholipid
T10877	37398-37407	GO_EXT:secretion_entity_or_process	denotes	secretion
T10878	37456-37463	UBERON:0002294	denotes	biliary
T10879	37464-37470	CHEBI:15889	denotes	sterol
T10880	37471-37480	GO_EXT:secretion_entity_or_process	denotes	secretion
T10881	37518-37525	UBERON:0002294	denotes	biliary
T10882	37526-37532	CHEBI:15889	denotes	sterol
T10883	37533-37542	GO_EXT:secretion_entity_or_process	denotes	secretion
T10884	37555-37559	NCBITaxon:10088	denotes	mice
T10885	37594-37619	CHEBI:80774	denotes	tauroursodeoxycholic acid
T10886	37621-37625	CHEBI:80774	denotes	TUDC
T10887	37628-37632	NCBITaxon:10088	denotes	Mice
T10888	37673-37677	UBERON:0001970	denotes	bile
T10889	37673-37682	CHEBI:22868	denotes	bile salt
T10890	37733-37745	UBERON:0004711	denotes	jugular vein
T10891	37771-37775	CHEBI:80774	denotes	TUDC
T10892	37800-37804	UBERON:0001970	denotes	bile
T10893	37800-37809	CHEBI:22868	denotes	bile salt
T10894	37810-37819	GO_EXT:secretion_entity_or_process	denotes	secretion
T10895	37859-37868	SO_EXT:genotype_or_entity_with_genotype	denotes	genotypes
T10896	37897-37901	CHEBI:80774	denotes	TUDC
T10897	37982-37991	GO_EXT:secretion_entity_or_process	denotes	secretion
T10898	37995-38007	CHEBI:16247	denotes	phospholipid
T10899	38053-38062	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T10900	38080-38084	NCBITaxon:10088	denotes	mice
T10901	38099-38103	NCBITaxon:10088	denotes	mice
T10902	38128-38140	CHEBI:16247	denotes	phospholipid
T10903	38141-38150	GO_EXT:secretion_entity_or_process	denotes	secretion
T10904	38234-38241	UBERON:0002294	denotes	biliary
T10905	38242-38248	CHEBI:15889	denotes	sterol
T10906	38249-38258	GO_EXT:secretion_entity_or_process	denotes	secretion
T10907	38272-38278	CHEBI:15889	denotes	sterol
T10908	38304-38317	CHEBI_GO_SO_EXT:enzyme	denotes	enzymatically
T10909	38367-38377	CHEBI:27693	denotes	sitosterol
T10910	38381-38392	CHEBI:16113	denotes	cholesterol
T10911	38437-38441	NCBITaxon:10088	denotes	mice
T10912	38464-38470	CHEBI:15889	denotes	sterol
T10913	38471-38480	GO_EXT:secretion_entity_or_process	denotes	secretion
T10914	38543-38547	NCBITaxon:10088	denotes	mice
T10915	38569-38573	NCBITaxon:10088	denotes	mice
T10916	38575-38581	CHEBI:15889	denotes	sterol
T10917	38582-38591	GO_EXT:secretion_entity_or_process	denotes	secretion
T10918	38647-38656	SO_EXT:wild_type_entity_or_quality	denotes	wild type
T10919	38673-38678	PR_EXT:000003573	denotes	Abcg8
T10920	38679-38689	PR_EXT:000003573	denotes	sterolin-2
T10921	38718-38725	UBERON:0002294	denotes	biliary
T10922	38727-38738	CHEBI:16113	denotes	cholesterol
T10923	38740-38749	GO_EXT:secretion_entity_or_process	denotes	secretion
T10924	38767-38771	NCBITaxon:10088	denotes	mice
T10925	38773-38779	CHEBI:15889	denotes	sterol
T10926	38780-38789	GO_EXT:secretion_entity_or_process	denotes	secretion
T10927	38887-38891	CHEBI:80774	denotes	TUDC
T10928	38903-38908	PR_EXT:000003573	denotes	Abcg8
T10929	38908-38909	SO_EXT:sequence_nullness_or_absence	denotes	-
T10930	38910-38911	SO_EXT:sequence_nullness_or_absence	denotes	-
T10931	38912-38916	NCBITaxon:10088	denotes	mice
T10932	38923-38931	GO:0046903	denotes	secreted
T10933	38937-38944	CHEBI:15889	denotes	sterols
T10934	38973-38980	UBERON:0002294	denotes	biliary
T10935	38981-38987	CHEBI:15889	denotes	sterol
T10936	39007-39015	GO:0046903	denotes	secreted
T10937	39027-39031	UBERON:0001970	denotes	bile
T10938	39051-39055	NCBITaxon:10088	denotes	mice
T10939	39086-39090	CHEBI:80774	denotes	TUDC
T10940	39111-39115	NCBITaxon:10088	denotes	Mice
T10941	39156-39160	UBERON:0001970	denotes	bile
T10942	39156-39165	CHEBI:3098	denotes	bile acid
T10943	39226-39230	CHEBI:80774	denotes	TUDC
T10944	39253-39257	UBERON_EXT:body	denotes	body
T10945	39280-39284	NCBITaxon:10088	denotes	mice
T10946	39330-39337	GO:0046903	denotes	secrete
T10947	39338-39349	CHEBI:16113	denotes	cholesterol
T10948	39387-39394	GO:0046903	denotes	secrete
T10949	39395-39405	CHEBI:27693	denotes	sitosterol
T10950	39410-39421	CHEBI:28623	denotes	campesterol
T10951	39434-39443	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T10952	39444-39448	NCBITaxon:10088	denotes	mice
T10953	39479-39484	PR_EXT:000003573	denotes	Abcg8
T10954	39484-39485	SO_EXT:normal_or_wild_type_or_present	denotes	+
T10955	39486-39487	SO_EXT:sequence_nullness_or_absence	denotes	-
T10956	39488-39492	NCBITaxon:10088	denotes	mice
T10957	39532-39539	GO:0046903	denotes	secrete
T10958	39544-39551	CHEBI:15889	denotes	sterols
T10959	39581-39590	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T10960	39591-39595	NCBITaxon:10088	denotes	mice
T12142	39689-39700	CHEBI:16113	denotes	cholesterol
T12143	39742-39753	CHEBI:16113	denotes	cholesterol
T12144	39754-39761	CHEBI:15889	denotes	sterols
T12145	39814-39819	UBERON:0002107	denotes	liver
T12146	39836-39843	GO:0046903	denotes	secrete
T12147	39844-39851	CHEBI:15889	denotes	sterols
T12148	39899-39906	SO_EXT:0000704	denotes	genetic
T12149	39927-39932	NCBITaxon:9606	denotes	human
T12150	40060-40072	GO_EXT:biological_mover_or_transporter	denotes	transporters
T12151	40138-40143	SO_EXT:0000704	denotes	genes
T12152	40256-40261	NCBITaxon:10088	denotes	mouse
T12153	40342-40347	SO_EXT:0000704	denotes	genes
T12154	40349-40354	PR_EXT:000003573	denotes	Abcg8
T12155	40361-40366	NCBITaxon:10088	denotes	mouse
T12156	40407-40412	NCBITaxon:9606	denotes	human
T12157	40438-40444	UBERON:0001969	denotes	Plasma
T12158	40453-40460	UBERON:0000479	denotes	tissues
T12159	40477-40482	UBERON:0000955	denotes	brain
T12160	40512-40522	CHEBI:27693	denotes	sitosterol
T12161	40551-40555	NCBITaxon:10088	denotes	mice
T12162	40651-40655	NCBITaxon:10088	denotes	mice
T12163	40660-40664	GO_EXT:breeding	denotes	bred
T12164	40802-40808	NCBITaxon:9606	denotes	humans
T12165	40826-40832	NCBITaxon:9606	denotes	humans
T12166	40850-40857	CHEBI:11814	denotes	HMG-CoA
T12167	40850-40867	GO_PR_EXT:hydroxymethylglutaryl_CoA_reductase	denotes	HMG-CoA reductase
T12168	40872-40878	PR_EXT:000006148	denotes	CYP7a1
T12169	40948-40952	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12170	40957-40963	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T12171	40982-40988	UBERON:0002107	denotes	livers
T12172	41003-41010	NCBITaxon:33208	denotes	animals
T12173	41091-41097	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T12174	41102-41106	UBERON:0001970	denotes	bile
T12175	41102-41111	CHEBI:3098	denotes	bile acid
T12176	41102-41121	GO:0006699	denotes	bile acid synthesis
T12177	41123-41129	PR_EXT:000006148	denotes	CYP7a1
T12178	41170-41174	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12179	41253-41263	CHEBI:27693	denotes	sitosterol
T12180	41276-41288	_FRAGMENT	denotes	inhibitor of
T12181	41294-41300	CHEBI_GO_EXT:enzyme_inhibitor	denotes	enzyme
T12182	41294-41300	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T12183	41316-41323	UBERON:0002294	denotes	Biliary
T12184	41324-41331	CHEBI:15889	denotes	sterols
T12185	41335-41340	PR_EXT:000003573	denotes	Abcg8
T12186	41351-41355	NCBITaxon:10088	denotes	mice
T12187	41396-41405	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12188	41406-41410	NCBITaxon:10088	denotes	mice
T12189	41427-41434	UBERON:0002294	denotes	biliary
T12190	41435-41441	CHEBI:15889	denotes	sterol
T12191	41442-41451	GO_EXT:secretion_entity_or_process	denotes	secretion
T12192	41461-41466	PR_EXT:000003573	denotes	Abcg8
T12193	41466-41467	SO_EXT:sequence_nullness_or_absence	denotes	-
T12194	41468-41469	SO_EXT:sequence_nullness_or_absence	denotes	-
T12195	41470-41474	NCBITaxon:10088	denotes	mice
T12196	41510-41516	CHEBI:15889	denotes	sterol
T12197	41517-41526	GO_EXT:secretion_entity_or_process	denotes	secretion
T12198	41532-41536	UBERON:0001970	denotes	bile
T12199	41567-41571	UBERON:0001970	denotes	bile
T12200	41567-41577	CHEBI:3098	denotes	bile acids
T12201	41609-41614	PR_EXT:000003573	denotes	Abcg8
T12202	41615-41625	PR_EXT:000003573	denotes	sterolin-2
T12203	41653-41660	GO:0046903	denotes	secrete
T12204	41661-41672	CHEBI:16113	denotes	cholesterol
T12205	41678-41682	UBERON:0001970	denotes	bile
T12206	41693-41702	GO_EXT:secretion_entity_or_process	denotes	secretion
T12207	41706-41716	CHEBI:27693	denotes	sitosterol
T12208	41761-41765	UBERON_EXT:body	denotes	body
T12209	41776-41782	UBERON:0001969	denotes	plasma
T12210	41783-41793	CHEBI:27693	denotes	sitosterol
T12211	41817-41821	NCBITaxon:10088	denotes	mice
T12212	41864-41871	UBERON:0002294	denotes	biliary
T12213	41872-41882	CHEBI:27693	denotes	sitosterol
T12214	41980-41990	CHEBI:27693	denotes	sitosterol
T12215	42009-42013	UBERON:0001970	denotes	bile
T12216	42028-42033	NCBITaxon:3193	denotes	plant
T12217	42034-42041	CHEBI:15889	denotes	sterols
T12218	42049-42057	GO:0046903	denotes	secreted
T12219	42073-42078	PR_EXT:000003573	denotes	Abcg8
T12220	42079-42089	PR_EXT:000003573	denotes	sterolin-2
T12221	42106-42111	PR_EXT:000003573	denotes	Abcg8
T12222	42111-42112	SO_EXT:sequence_nullness_or_absence	denotes	-
T12223	42113-42114	SO_EXT:sequence_nullness_or_absence	denotes	-
T12224	42115-42119	NCBITaxon:10088	denotes	mice
T12225	42123-42130	GO:0046903	denotes	secrete
T12226	42131-42135	UBERON:0001970	denotes	bile
T12227	42131-42140	CHEBI:22868	denotes	bile salt
T12228	42145-42157	CHEBI:16247	denotes	phospholipid
T12229	42163-42167	UBERON:0001970	denotes	bile
T12230	42183-42192	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12231	42193-42197	NCBITaxon:10088	denotes	mice
T12232	42267-42272	PR_EXT:000003573	denotes	Abcg8
T12233	42273-42283	PR_EXT:000003573	denotes	sterolin-2
T12234	42301-42314	UBERON:0002423	denotes	hepatobiliary
T12235	42315-42326	CHEBI:16113	denotes	cholesterol
T12236	42327-42336	GO_EXT:secretion_entity_or_process	denotes	secretion
T12237	42340-42344	NCBITaxon:10088	denotes	mice
T12238	42346-42351	PR_EXT:000003573	denotes	Abcg8
T12239	42351-42352	SO_EXT:sequence_nullness_or_absence	denotes	-
T12240	42353-42354	SO_EXT:sequence_nullness_or_absence	denotes	-
T12241	42355-42359	NCBITaxon:10088	denotes	mice
T12242	42412-42418	NCBITaxon:9989	denotes	rodent
T12243	42419-42423	CHEBI_EXT:33290	denotes	chow
T12244	42506-42511	NCBITaxon:10088	denotes	mouse
T12245	42530-42539	PR_EXT:sterolin	denotes	sterolins
T12246	42563-42569	UBERON:0001969	denotes	Plasma
T12247	42574-42581	UBERON:0002107	denotes	hepatic
T12248	42582-42587	NCBITaxon:3193	denotes	plant
T12249	42588-42594	CHEBI:15889	denotes	sterol
T12250	42605-42610	PR_EXT:000003573	denotes	Abcg8
T12251	42610-42611	SO_EXT:sequence_nullness_or_absence	denotes	-
T12252	42612-42613	SO_EXT:sequence_nullness_or_absence	denotes	-
T12253	42618-42623	PR_EXT:000003572	denotes	Abcg5
T12254	42624-42629	PR_EXT:000003573	denotes	Abcg8
T12255	42688-42699	CHEBI:16113	denotes	cholesterol
T12256	42723-42729	UBERON:0001969	denotes	plasma
T12257	42730-42742	CHEBI:17855	denotes	triglyceride
T12258	42757-42762	PR_EXT:000003573	denotes	Abcg8
T12259	42762-42763	SO_EXT:sequence_nullness_or_absence	denotes	-
T12260	42764-42765	SO_EXT:sequence_nullness_or_absence	denotes	-
T12261	42766-42770	NCBITaxon:10088	denotes	mice
T12262	42797-42806	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12263	42807-42814	NCBITaxon:33208	denotes	animals
T12264	42834-42846	CHEBI:17855	denotes	triglyceride
T12265	42865-42868	CHEBI_GO_EXT:low_density_lipoprotein_particle	denotes	LDL
T12266	42966-42969	CHEBI:8984	denotes	SDS
T12267	43046-43055	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12268	43078-43085	UBERON:0002107	denotes	Hepatic
T12269	43108-43119	CHEBI:16113	denotes	cholesterol
T12270	43144-43149	PR_EXT:000003573	denotes	Abcg8
T12271	43149-43150	SO_EXT:sequence_nullness_or_absence	denotes	-
T12272	43151-43152	SO_EXT:sequence_nullness_or_absence	denotes	-
T12273	43153-43157	NCBITaxon:10088	denotes	mice
T12274	43170-43179	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12275	43180-43184	NCBITaxon:10088	denotes	mice
T12276	43190-43194	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12277	43205-43212	CHEBI:11814	denotes	HMG-CoA
T12278	43205-43222	GO_PR_EXT:hydroxymethylglutaryl_CoA_reductase	denotes	HMG-CoA reductase
T12279	43227-43233	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T12280	43294-43298	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12281	43306-43312	PR_EXT:000028988	denotes	Srebps
T12282	43385-43390	NCBITaxon:3193	denotes	plant
T12283	43391-43398	CHEBI:15889	denotes	sterols
T12284	43425-43430	PR_EXT:000028988	denotes	SREBP
T12285	43443-43453	CHEBI:27693	denotes	Sitosterol
T12286	43489-43495	PR_EXT:000006148	denotes	Cyp7a1
T12287	43562-43568	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T12288	43622-43626	NCBITaxon:10088	denotes	mice
T12289	43647-43657	CHEBI:27693	denotes	sitosterol
T12290	43685-43691	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T12291	43708-43712	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12292	43818-43824	CHEBI_GO_SO_EXT:enzyme	denotes	enzyme
T12293	43829-43833	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12294	43853-43857	NCBITaxon:10088	denotes	mice
T12295	43876-43880	NCBITaxon:10088	denotes	mice
T12296	43902-43913	CHEBI:16113	denotes	cholesterol
T12297	43919-43929	CHEBI:27693	denotes	sitosterol
T12298	43983-43990	CHEBI:15889	denotes	sterols
T12299	43998-44002	UBERON_EXT:body	denotes	body
T12300	44037-44042	NCBITaxon:9606	denotes	human
T12301	44090-44101	NCBITaxon:1	denotes	individuals
T12302	44122-44137	UBERON:0001621	denotes	coronary artery
T12303	44147-44152	PR_EXT:000003573	denotes	Abcg8
T12304	44153-44163	PR_EXT:000003573	denotes	sterolin-2
T12305	44174-44178	NCBITaxon:10088	denotes	mice
T12306	44221-44228	CHEBI_PR_EXT:protein	denotes	protein
T12307	44232-44239	UBERON:0002294	denotes	biliary
T12309	44247-44256	GO_EXT:secretion_entity_or_process	denotes	secretion
T12310	44262-44266	UBERON:0001970	denotes	bile
T12311	44270-44276	CHEBI:15889	denotes	sterol
T12312	44317-44321	UBERON:0001970	denotes	bile
T12313	44317-44326	CHEBI:22868	denotes	bile salt
T12314	44327-44336	GO:0007588	denotes	excretion
T12315	44338-44343	PR_EXT:000003573	denotes	Abcg8
T12316	44344-44354	PR_EXT:000003573	denotes	sterolin-2
T12317	44365-44369	NCBITaxon:10088	denotes	mice
T12318	44377-44384	GO_EXT:reaction_or_response	denotes	respond
T12319	44405-44414	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12320	44415-44419	NCBITaxon:10088	denotes	mice
T12321	44427-44432	PR_EXT:000003573	denotes	Abcg8
T12322	44433-44443	PR_EXT:000003573	denotes	sterolin-2
T12323	44461-44472	CHEBI:16113	denotes	cholesterol
T12324	44473-44482	GO_EXT:secretion_entity_or_process	denotes	secretion
T12325	44505-44510	NCBITaxon:3193	denotes	plant
T12326	44511-44517	CHEBI:15889	denotes	sterol
T12327	44518-44527	GO_EXT:secretion_entity_or_process	denotes	secretion
T12328	44566-44573	UBERON:0002294	denotes	biliary
T12329	44574-44580	CHEBI:15889	denotes	sterol
T12330	44618-44627	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12331	44628-44632	NCBITaxon:10088	denotes	mice
T12332	44677-44683	UBERON:0000479	denotes	tissue
T12333	44688-44694	UBERON:0001969	denotes	plasma
T12334	44704-44714	CHEBI:27693	denotes	sitosterol
T12335	44747-44751	NCBITaxon:10088	denotes	mice
T12336	44790-44800	CHEBI:27693	denotes	sitosterol
T12337	44834-44838	UBERON:0001970	denotes	bile
T12338	44883-44892	PR_EXT:sterolin	denotes	sterolins
T12339	44898-44904	GO_RO_EXT:biological_export	denotes	export
T12340	44920-44927	CHEBI:15889	denotes	sterols
T12341	44953-44959	NCBITaxon:9606	denotes	humans
T12342	44985-44992	SO_EXT:0000704	denotes	genetic
T12343	45011-45016	PR_EXT:000003572	denotes	ABCG5
T12344	45020-45025	PR_EXT:000003573	denotes	ABCG8
T12345	45060-45064	NCBITaxon:10088	denotes	mice
T12346	45082-45087	PR_EXT:000003573	denotes	Abcg8
T12347	45088-45098	PR_EXT:000003573	denotes	sterolin-2
T12348	45120-45126	CHEBI:15889	denotes	sterol
T12349	45127-45136	GO_EXT:secretion_entity_or_process	denotes	secretion
T12350	45211-45217	NCBITaxon:9989	denotes	rodent
T12351	45218-45222	CHEBI_EXT:33290	denotes	chow
T12352	45242-45249	NCBITaxon:33208	denotes	animals
T12353	45284-45290	UBERON:0001969	denotes	plasma
T12354	45294-45300	UBERON:0000479	denotes	tissue
T12355	45301-45311	CHEBI:27693	denotes	sitosterol
T12356	45358-45366	CHEBI_PR_EXT:protein	denotes	proteins
T12357	45423-45428	PR_EXT:000003573	denotes	Abcg8
T12358	45428-45429	SO_EXT:normal_or_wild_type_or_present	denotes	+
T12359	45430-45431	SO_EXT:sequence_nullness_or_absence	denotes	-
T12360	45432-45436	NCBITaxon:10088	denotes	mice
T12361	45461-45468	UBERON:0002294	denotes	biliary
T12362	45469-45479	CHEBI:27693	denotes	sitosterol
T12363	45496-45505	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12364	45506-45510	NCBITaxon:10088	denotes	mice
T12365	45536-45543	UBERON:0002294	denotes	biliary
T12366	45544-45550	CHEBI:15889	denotes	sterol
T12367	45551-45560	GO_EXT:secretion_entity_or_process	denotes	secretion
T12368	45584-45593	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12369	45594-45598	NCBITaxon:10088	denotes	mice
T12370	45623-45627	NCBITaxon:10088	denotes	mice
T12371	45681-45689	CHEBI_PR_EXT:protein	denotes	proteins
T12372	45799-45806	GO:0007631	denotes	feeding
T12373	45847-45856	GO_EXT:secretion_entity_or_process	denotes	secretion
T12374	45937-45943	UBERON:0000479	denotes	tissue
T12375	45948-45954	UBERON:0001969	denotes	plasma
T12376	45955-45960	NCBITaxon:3193	denotes	plant
T12377	45961-45967	CHEBI:15889	denotes	sterol
T12378	45991-45998	NCBITaxon:33208	denotes	animals
T12379	46049-46056	NCBITaxon:33208	denotes	animals
T12380	46067-46072	NCBITaxon:3193	denotes	plant
T12381	46073-46079	CHEBI:15889	denotes	sterol
T12382	46103-46109	UBERON:0001969	denotes	plasma
T12383	46110-46116	CHEBI:15889	denotes	sterol
T12384	46164-46169	PR_EXT:000003572	denotes	Abcg5
T12385	46170-46175	PR_EXT:000003573	denotes	Abcg8
T12386	46176-46180	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12387	46181-46191	GO:0010467	denotes	expression
T12388	46218-46223	NCBITaxon:10088	denotes	mouse
T12389	46248-46255	UBERON:0002294	denotes	biliary
T12390	46256-46267	CHEBI:16113	denotes	cholesterol
T12391	46268-46277	GO_EXT:secretion_entity_or_process	denotes	secretion
T12392	46317-46326	CHEBI:4629	denotes	diosgenin
T12393	46327-46330	GO:0007631	denotes	fed
T12394	46331-46335	NCBITaxon:10088	denotes	mice
T12395	46364-46371	UBERON:0002294	denotes	biliary
T12396	46372-46383	CHEBI:16113	denotes	cholesterol
T12397	46392-46396	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12398	46407-46412	PR_EXT:000003572	denotes	Abcg5
T12399	46417-46422	PR_EXT:000003573	denotes	Abcg8
T12400	46474-46481	CHEBI_PR_EXT:protein	denotes	protein
T12401	46491-46496	PR_EXT:000003572	denotes	Abcg5
T12402	46521-46530	CHEBI:4629	denotes	diosgenin
T12403	46541-46546	PR_EXT:000003573	denotes	Abcg8
T12404	46606-46613	UBERON:0002294	denotes	biliary
T12405	46614-46625	CHEBI:16113	denotes	cholesterol
T12406	46626-46635	GO_EXT:secretion_entity_or_process	denotes	secretion
T12407	46694-46701	UBERON:0002294	denotes	biliary
T12408	46702-46713	CHEBI:16113	denotes	cholesterol
T12409	46714-46723	GO_EXT:secretion_entity_or_process	denotes	secretion
T12410	46756-46761	PR_EXT:000003573	denotes	Abcg8
T12411	46772-46776	NCBITaxon:10088	denotes	mice
T12412	46798-46808	PR_EXT:000003573	denotes	sterolin-2
T12413	46850-46857	SO_EXT:0000704	denotes	genetic
T12414	46868-46874	UBERON:0001969	denotes	plasma
T12415	46875-46880	NCBITaxon:3193	denotes	plant
T12416	46881-46887	CHEBI:15889	denotes	sterol
T12417	46961-46972	GO:0065007	denotes	controlling
T12418	46973-46979	UBERON:0001969	denotes	plasma
T12419	46980-46985	NCBITaxon:3193	denotes	plant
T12420	46986-46992	CHEBI:15889	denotes	sterol
T12421	47040-47046	NCBITaxon:39107	denotes	murine
T12422	47135-47145	GO:0065007	denotes	regulating
T12423	47146-47152	UBERON:0001969	denotes	plasma
T12424	47153-47158	NCBITaxon:3193	denotes	plant
T12425	47159-47165	CHEBI:15889	denotes	sterol
T12426	47246-47252	CHEBI:15889	denotes	sterol
T12427	47246-47264	GO:0015918	denotes	sterol trafficking
T12428	47359-47364	PR_EXT:000003572	denotes	Abcg5
T12429	47365-47375	PR_EXT:000003572	denotes	sterolin-1
T12430	47380-47385	PR_EXT:000003573	denotes	Abcg8
T12431	47386-47396	PR_EXT:000003573	denotes	sterolin-2
T12432	47427-47433	GO_EXT:dimer_macromolecular_complex	denotes	dimers
T12433	47453-47458	PR_EXT:000003573	denotes	Abcg8
T12434	47459-47469	PR_EXT:000003573	denotes	sterolin-2
T12435	47519-47528	PR_EXT:sterolin	denotes	sterolins
T12436	47553-47558	PR_EXT:000003573	denotes	Abcg8
T12437	47559-47569	PR_EXT:000003573	denotes	sterolin-2
T12438	47570-47580	GO:0010467	denotes	expression
T12439	47605-47611	GO:0045177	denotes	apical
T12440	47612-47623	GO_EXT:biological_routing_or_transport	denotes	trafficking
T12441	47627-47632	PR_EXT:000003572	denotes	Abcg5
T12442	47633-47643	PR_EXT:000003572	denotes	sterolin-1
T12443	47651-47657	GO:0045177	denotes	apical
T12444	47658-47665	GO:0009986	denotes	surface
T12445	47681-47685	CL_GO_EXT:cell	denotes	cell
T12446	47725-47735	GO:0010467	denotes	expression
T12447	47822-47827	PR_EXT:000003572	denotes	Abcg5
T12448	47828-47838	PR_EXT:000003572	denotes	sterolin-1
T12449	47839-47849	GO:0042571	denotes	antibodies
T12450	47900-47911	GO_EXT:biological_routing_or_transport	denotes	trafficking
T12451	47915-47920	PR_EXT:000003572	denotes	Abcg5
T12452	47921-47931	PR_EXT:000003572	denotes	sterolin-1
T12453	47935-47940	PR_EXT:000003573	denotes	Abcg8
T12454	47940-47941	SO_EXT:sequence_nullness_or_absence	denotes	-
T12455	47942-47943	SO_EXT:sequence_nullness_or_absence	denotes	-
T12456	47944-47948	NCBITaxon:10088	denotes	mice
T12457	47971-47986	MOP:0002162	denotes	N-glycosylation
T12458	47973-47986	GO_MOP_EXT:glycosylation	denotes	glycosylation
T12459	47987-47997	GO_PATO_EXT:biological_maturation	denotes	maturation
T12460	48019-48024	PR_EXT:000003572	denotes	Abcg5
T12461	48025-48035	PR_EXT:000003572	denotes	sterolin-1
T12462	48044-48049	PR_EXT:000003573	denotes	Abcg8
T12463	48049-48050	SO_EXT:sequence_nullness_or_absence	denotes	-
T12464	48051-48052	SO_EXT:sequence_nullness_or_absence	denotes	-
T12465	48053-48057	NCBITaxon:10088	denotes	mice
T12466	48077-48098	GO:0005783	denotes	endoplasmic reticulum
T12467	48149-48154	PR_EXT:000003572	denotes	Abcg5
T12468	48155-48165	PR_EXT:000003572	denotes	sterolin-1
T12469	48169-48177	GO:0045177	denotes	apically
T12470	48178-48187	GO:0010467	denotes	expressed
T12471	48195-48200	PR_EXT:000003573	denotes	Abcg8
T12472	48211-48215	NCBITaxon:10088	denotes	mice
T12473	48239-48244	PR_EXT:000003572	denotes	Abcg5
T12474	48245-48255	PR_EXT:000003572	denotes	sterolin-1
T12475	48256-48264	GO:0042571	denotes	antibody
T12476	48301-48306	PR_EXT:000003572	denotes	Abcg5
T12477	48307-48312	PR_EXT:000003573	denotes	Abcg8
T12478	48313-48325	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T12479	48329-48335	NCBITaxon:9608	denotes	canine
T12480	48336-48358	UBERON:0002029	denotes	gallbladder epithelial
T12481	48336-48364	CL:1000415	denotes	gallbladder epithelial cells
T12482	48359-48364	CL_GO_EXT:cell	denotes	cells
T12483	48376-48384	CHEBI_PR_EXT:protein	denotes	proteins
T12484	48396-48411	GO:0005622	denotes	intracellularly
T12485	48401-48411	CL_GO_EXT:cell	denotes	cellularly
T12486	48459-48464	UBERON:0002107	denotes	liver
T12487	48459-48481	PR_EXT:000011395	denotes	liver X receptor alpha
T12488	48459-48475	_FRAGMENT	denotes	liver X receptor
T12489	48489-48496	CHEBI_EXT:86029	denotes	agonist
T12490	48467-48475	GO_EXT:0004872	denotes	receptor
T12491	48483-48487	PR_EXT:000011395	denotes	LXRα
T12492	48489-48496	CHEBI_GO_EXT:agonist	denotes	agonist
T12493	48498-48503	PR_EXT:000003572	denotes	Abcg5
T12494	48504-48514	PR_EXT:000003572	denotes	sterolin-1
T12495	48519-48524	PR_EXT:000003573	denotes	Abcg8
T12496	48525-48535	PR_EXT:000003573	denotes	sterolin-2
T12497	48551-48560	GO:0010467	denotes	expressed
T12498	48568-48574	GO:0045177	denotes	apical
T12499	48575-48582	GO:0009986	denotes	surface
T12500	48615-48620	PR_EXT:000003572	denotes	Abcg5
T12501	48621-48631	PR_EXT:000003572	denotes	sterolin-1
T12502	48632-48640	GO:0042571	denotes	antibody
T12503	48703-48714	GO_EXT:biological_routing_or_transport	denotes	trafficking
T12504	48724-48736	GO_EXT:biological_mover_or_transporter	denotes	transporters
T12505	48811-48817	GO:0045177	denotes	apical
T12506	48829-48832	NCBITaxon:10114	denotes	rat
T12507	48833-48837	PR_EXT:000015052	denotes	SPNT
T12508	48851-48867	UBERON:0004819	denotes	renal epithelial
T12509	48851-48873	CL:0002518	denotes	renal epithelial cells
T12510	48868-48873	CL_GO_EXT:cell	denotes	cells
T12511	48893-48908	MOP:0002162	denotes	N-glycosylation
T12512	48895-48908	GO_MOP_EXT:glycosylation	denotes	glycosylation
T12513	48984-48989	PR_EXT:000003572	denotes	Abcg5
T12514	48990-49000	PR_EXT:000003572	denotes	sterolin-1
T12515	49001-49011	GO:0010467	denotes	expression
T12516	49033-49038	PR_EXT:000003573	denotes	Abcg8
T12517	49039-49049	PR_EXT:000003573	denotes	sterolin-2
T12518	49060-49064	NCBITaxon:10088	denotes	mice
T12519	49127-49135	CHEBI_PR_EXT:protein	denotes	proteins
T12520	49258-49263	PR_EXT:000003572	denotes	Abcg5
T12521	49264-49274	PR_EXT:000003572	denotes	sterolin-1
T12522	49282-49287	PR_EXT:000003573	denotes	Abcg8
T12523	49287-49288	SO_EXT:sequence_nullness_or_absence	denotes	-
T12524	49289-49290	SO_EXT:sequence_nullness_or_absence	denotes	-
T12525	49291-49295	NCBITaxon:10088	denotes	mice
T12526	49346-49351	PR_EXT:000003572	denotes	Abcg5
T12527	49352-49362	PR_EXT:000003572	denotes	sterolin-1
T12528	49373-49378	NCBITaxon:10088	denotes	mouse
T12529	49409-49416	GO:0046903	denotes	secrete
T12530	49417-49424	UBERON:0002294	denotes	biliary
T12531	49425-49436	CHEBI:16113	denotes	cholesterol
T12532	49463-49472	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12533	49473-49477	NCBITaxon:10088	denotes	mice
T12534	49488-49491	GO:0007631	denotes	fed
T12535	49494-49497	PR_EXT:liver_X_receptor	denotes	LXR
T12536	49494-49505	CHEBI_EXT:86029	denotes	LXR agonist
T12537	49498-49505	CHEBI_GO_EXT:agonist	denotes	agonist
T12538	49518-49523	PR_EXT:000003573	denotes	Abcg8
T12539	49524-49528	CHEBI_SO_EXT:mRNA	denotes	mRNA
T12540	49529-49539	GO:0010467	denotes	expression
T12541	49554-49561	GO:0046903	denotes	secrete
T12542	49567-49574	UBERON:0002294	denotes	biliary
T12543	49575-49586	CHEBI:16113	denotes	cholesterol
T12544	49592-49601	SO_EXT:wild_type_entity_or_quality	denotes	wild-type
T12545	49602-49606	NCBITaxon:10088	denotes	mice
T12546	49637-49642	PR_EXT:000003572	denotes	Abcg5
T12547	49643-49653	PR_EXT:000003572	denotes	sterolin-1
T12548	49658-49663	PR_EXT:000003573	denotes	Abcg8
T12549	49664-49674	PR_EXT:000003573	denotes	sterolin-2
T12550	49709-49718	GO_EXT:secretion_entity_or_process	denotes	secretion
T12551	49722-49729	UBERON:0002107	denotes	hepatic
T12552	49730-49737	CHEBI:15889	denotes	sterols
T12553	49743-49747	UBERON:0001970	denotes	bile
T12554	49797-49802	PR_EXT:000003572	denotes	Abcg5
T12555	49813-49818	NCBITaxon:10088	denotes	mouse
T12556	49904-49915	GO_PATO_EXT:physiological_process_or_quality	denotes	physiologic
T12557	49985-49991	NCBITaxon:33208	denotes	animal
T12558	50012-50017	PR_EXT:000003572	denotes	Abcg5
T12559	50018-50028	PR_EXT:000003572	denotes	sterolin-1
T12560	50033-50038	PR_EXT:000003573	denotes	Abcg8
T12561	50039-50049	PR_EXT:000003573	denotes	sterolin-2
T12562	50102-50110	CHEBI_PR_EXT:protein	denotes	proteins
T12563	50126-50135	PR_EXT:sterolin	denotes	sterolins
T12564	50145-50152	GO:0046903	denotes	secrete
T12565	50153-50160	UBERON:0002294	denotes	biliary
T12566	50161-50168	CHEBI:15889	denotes	sterols
T14655	50259-50264	SO_EXT:0000704	denotes	genes
T14656	50365-50370	PR_EXT:000003573	denotes	Abcg8
T14657	50371-50381	PR_EXT:000003573	denotes	sterolin-2
T14658	50403-50410	UBERON:0002294	denotes	biliary
T14659	50411-50422	CHEBI:16113	denotes	cholesterol
T14660	50423-50432	GO_EXT:secretion_entity_or_process	denotes	secretion
T14661	50441-50446	PR_EXT:000003572	denotes	Abcg5
T14662	50447-50457	PR_EXT:000003572	denotes	sterolin-1
T14663	50478-50486	GO:0045177	denotes	apically
T14664	50487-50496	GO:0010467	denotes	expressed
T14665	50520-50525	PR_EXT:000003573	denotes	Abcg8
T14666	50526-50536	PR_EXT:000003573	denotes	sterolin-2
T14667	50585-50590	PR_EXT:000003573	denotes	Abcg8
T14668	50591-50601	PR_EXT:000003573	denotes	sterolin-2
T14669	50621-50628	UBERON:0002294	denotes	biliary
T14670	50629-50640	CHEBI:16113	denotes	cholesterol
T14671	50641-50650	GO_EXT:secretion_entity_or_process	denotes	secretion
T14672	50661-50668	UBERON:0002294	denotes	biliary
T14673	50669-50679	CHEBI:27693	denotes	sitosterol
T14674	50680-50689	GO_EXT:secretion_entity_or_process	denotes	secretion
T14675	50772-50777	PR_EXT:000003573	denotes	Abcg8
T14676	50778-50788	PR_EXT:000003573	denotes	sterolin-2
T14677	50808-50817	GO_EXT:secretion_entity_or_process	denotes	secretion
T14678	50821-50831	CHEBI:27693	denotes	sitosterol
T14679	50837-50841	UBERON:0001970	denotes	bile
T8098	30305-30312	CHEBI:15889	denotes	sterols
T12308	44240-44246	CHEBI:15889	denotes	sterol
R4953	T7420	T7419	_lexicallyChainedTo	protein,production of
R5280	T8072	T8071	_lexicallyChainedTo	sterols,entry of
R5281	T8128	T8127	_lexicallyChainedTo	E,apolipoproteins
R5282	T8129	T8127	_lexicallyChainedTo	AI,apolipoproteins
R5283	T8130	T8127	_lexicallyChainedTo	AII,apolipoproteins
R7546	T12181	T12180	_lexicallyChainedTo	enzyme,inhibitor of
R7547	T12489	T12488	_lexicallyChainedTo	agonist,liver X receptor
R356	T912	T911	_lexicallyChainedTo	cholesterol from,Absorption of
R357	T913	T912	_lexicallyChainedTo	intestine,cholesterol from
R358	T977	T976	_lexicallyChainedTo	transporter,sterol
R2314	T3654	T3653	_lexicallyChainedTo	DNA,amplification of

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id	Subject	Object	Predicate	Lexical cue
T79	2-7	NCBITaxon:10088	denotes	mouse
T80	44-49	PR:000003573	denotes	Abcg8
T81	50-60	PR:000003573	denotes	sterolin-2
T82	83-90	GO:0046903	denotes	secrete
T83	91-98	UBERON:0002294	denotes	biliary
T84	99-110	CHEBI:16113	denotes	cholesterol
T85	159-164	SO:0000704	denotes	genes
T86	232-237	PR:000003572	denotes	ABCG5
T87	248-258	PR:000003572	denotes	sterolin-1
T88	264-269	PR:000003573	denotes	ABCG8
T89	280-290	PR:000003573	denotes	sterolin-2
T90	326-335	GO:0030849	denotes	autosomal
T91	358-364	CHEBI:15889	denotes	sterol
T92	358-376	GO:0015918	denotes	sterol trafficking
T93	404-410	UBERON:0001969	denotes	plasma
T94	411-416	NCBITaxon:3193	denotes	plant
T95	417-423	CHEBI:15889	denotes	sterol
T96	475-480	PR:000003572	denotes	ABCG5
T97	481-491	PR:000003572	denotes	sterolin-1
T98	496-501	PR:000003573	denotes	ABCG8
T99	502-512	PR:000003573	denotes	sterolin-2
T100	619-625	NCBITaxon:9606	denotes	humans
T101	658-663	PR:000003572	denotes	ABCG5
T102	667-672	PR:000003573	denotes	ABCG8
T103	716-722	NCBITaxon:9606	denotes	humans
T104	744-754	GO:0065007	denotes	regulating
T105	763-769	CHEBI:15889	denotes	sterol
T106	763-775	GO:0035376	denotes	sterol entry
T107	780-787	UBERON:0002294	denotes	biliary
T108	788-794	CHEBI:15889	denotes	sterol
T109	837-842	NCBITaxon:9606	denotes	human
T110	892-897	NCBITaxon:10088	denotes	mouse
T111	935-940	PR:000003573	denotes	Abcg8
T112	941-951	PR:000003573	denotes	sterolin-2
T113	990-994	NCBITaxon:10088	denotes	mice
T114	1043-1047	NCBITaxon:10088	denotes	Mice
T115	1061-1066	PR:000003573	denotes	Abcg8
T116	1096-1102	UBERON:0001969	denotes	plasma
T117	1107-1113	UBERON:0000479	denotes	tissue
T118	1114-1119	NCBITaxon:3193	denotes	plant
T119	1120-1126	CHEBI:15889	denotes	sterol
T120	1135-1145	CHEBI:27693	denotes	sitosterol
T121	1150-1161	CHEBI:28623	denotes	campesterol
T122	1210-1215	PR:000003572	denotes	Abcg5
T123	1216-1226	PR:000003572	denotes	sterolin-1
T124	1231-1240	GO:0010467	denotes	expressed
T125	1249-1254	UBERON:0002107	denotes	liver
T126	1259-1268	UBERON:0000160	denotes	intestine
T127	1272-1277	PR:000003573	denotes	Abcg8
T128	1278-1288	PR:000003573	denotes	sterolin-2
T129	1299-1303	NCBITaxon:10088	denotes	mice
T130	1329-1335	GO:0045177	denotes	apical
T131	1336-1346	GO:0010467	denotes	expression
T132	1360-1365	PR:000003573	denotes	Abcg8
T133	1376-1380	NCBITaxon:10088	denotes	mice
T134	1408-1415	GO:0046903	denotes	secrete
T135	1416-1427	CHEBI:16113	denotes	cholesterol
T136	1433-1437	UBERON:0001970	denotes	bile
T137	1475-1482	GO:0046903	denotes	secrete
T138	1483-1493	CHEBI:27693	denotes	sitosterol
T139	1556-1561	PR:000003573	denotes	Abcg8
T140	1565-1569	NCBITaxon:10088	denotes	mice
T141	1629-1636	UBERON:0002294	denotes	biliary
T142	1637-1643	CHEBI:15889	denotes	sterol
T143	1676-1680	NCBITaxon:10088	denotes	mice
T144	1719-1724	PR:000003573	denotes	Abcg8
T145	1725-1735	PR:000003573	denotes	sterolin-2
T146	1753-1760	UBERON:0002294	denotes	biliary
T147	1761-1767	CHEBI:15889	denotes	sterol
T148	1795-1800	PR:000003573	denotes	Abcg8
T149	1801-1811	PR:000003573	denotes	sterolin-2
T150	1844-1851	UBERON:0002294	denotes	biliary
T151	1852-1863	CHEBI:16113	denotes	cholesterol
T152	1879-1889	CHEBI:27693	denotes	sitosterol
T153	1897-1904	UBERON:0002294	denotes	biliary
T154	1905-1915	CHEBI:27693	denotes	sitosterol
T155	1984-1988	NCBITaxon:10088	denotes	mice
T156	2046-2051	PR:000003573	denotes	Abcg8
T157	2052-2062	PR:000003573	denotes	sterolin-2
T158	2105-2109	UBERON:0001970	denotes	bile
T782	2123-2136	_FRAGMENT	denotes	Absorption of
T783	2145-2161	_FRAGMENT	denotes	cholesterol from
T784	2166-2175	GO:0030299	denotes	intestine
T785	2145-2156	CHEBI:16113	denotes	cholesterol
T786	2166-2175	UBERON:0000160	denotes	intestine
T787	2200-2211	CHEBI:16113	denotes	cholesterol
T788	2200-2223	GO:0042632	denotes	cholesterol homeostasis
T789	2276-2287	CHEBI:16113	denotes	cholesterol
T790	2301-2310	GO:0008152	denotes	metabolic
T791	2380-2391	CHEBI:16113	denotes	cholesterol
T792	2400-2411	CHEBI:16113	denotes	cholesterol
T793	2412-2419	CHEBI:15889	denotes	sterols
T794	2428-2433	NCBITaxon:3193	denotes	plant
T795	2434-2441	CHEBI:15889	denotes	sterols
T796	2477-2488	CHEBI:16113	denotes	cholesterol
T797	2549-2560	CHEBI:16113	denotes	cholesterol
T798	2561-2568	CHEBI:15889	denotes	sterols
T799	2633-2644	CHEBI:16113	denotes	cholesterol
T800	2654-2665	CHEBI:16113	denotes	cholesterol
T801	2666-2673	CHEBI:15889	denotes	sterols
T802	2694-2703	UBERON:0000160	denotes	intestine
T803	2767-2778	CHEBI:16113	denotes	cholesterol
T804	2856-2861	UBERON:0002107	denotes	liver
T805	2862-2870	GO:0046903	denotes	secretes
T806	2876-2887	CHEBI:16113	denotes	cholesterol
T807	2893-2897	UBERON:0001970	denotes	bile
T808	2921-2928	GO:0007588	denotes	excrete
T809	2933-2944	CHEBI:16113	denotes	cholesterol
T810	2945-2952	CHEBI:15889	denotes	sterols
T811	2958-2962	UBERON:0001970	denotes	bile
T812	3077-3082	NCBITaxon:9606	denotes	human
T813	3203-3212	GO:0030849	denotes	autosomal
T814	3271-3276	UBERON:0000178	denotes	blood
T815	3281-3287	UBERON:0000479	denotes	tissue
T816	3288-3293	NCBITaxon:3193	denotes	plant
T817	3294-3300	CHEBI:15889	denotes	sterol
T818	3322-3333	NCBITaxon:1	denotes	individuals
T819	3346-3352	UBERON:0000043	denotes	tendon
T820	3360-3368	UBERON:4200132	denotes	tuberous
T821	3514-3520	UBERON:0001969	denotes	plasma
T822	3521-3526	NCBITaxon:3193	denotes	plant
T823	3527-3533	CHEBI:15889	denotes	sterol
T824	3555-3565	CHEBI:27693	denotes	sitosterol
T825	3567-3578	CHEBI:28623	denotes	campesterol
T826	3580-3592	CHEBI:28824	denotes	stigmasterol
T827	3646-3657	CHEBI:25212	denotes	metabolites
T828	3725-3736	CHEBI:16113	denotes	cholesterol
T829	3787-3798	NCBITaxon:1	denotes	individuals
T830	3823-3830	CHEBI:15889	denotes	sterols
T831	3875-3881	CHEBI:15889	denotes	sterol
T832	3929-3936	GO:0007588	denotes	excrete
T833	3937-3944	CHEBI:15889	denotes	sterols
T834	3950-3954	UBERON:0001970	denotes	bile
T835	3974-3984	CHEBI:27693	denotes	sitosterol
T836	3989-4000	CHEBI:16113	denotes	cholesterol
T837	4013-4024	NCBITaxon:1	denotes	individuals
T838	4059-4068	GO:0007588	denotes	excretion
T839	4072-4082	CHEBI:27693	denotes	sitosterol
T840	4088-4092	UBERON:0001970	denotes	bile
T841	4116-4127	NCBITaxon:1	denotes	individuals
T842	4128-4135	UBERON:0002294	denotes	biliary
T843	4136-4142	CHEBI:15889	denotes	sterol
T844	4257-4263	CHEBI:15889	denotes	sterol
T845	4278-4287	GO:0010467	denotes	expressed
T846	4295-4318	UBERON:0012427	denotes	intestinal brush border
T847	4330-4349	UBERON:0001283	denotes	hepatic canalicular
T848	4368-4375	SO:0000704	denotes	Genetic
T849	4454-4459	NCBITaxon:9606	denotes	human
T850	4573-4578	SO:0000704	denotes	genes
T851	4602-4614	CHEBI:86029	denotes	LXR agonists
T852	4654-4659	SO:0000704	denotes	genes
T853	4661-4666	PR:000003572	denotes	ABCG5
T854	4671-4676	PR:000003573	denotes	ABCG8
T855	4704-4714	PR:000003572	denotes	sterolin-1
T856	4719-4729	PR:000003573	denotes	sterolin-2
T857	4823-4828	PR:000003572	denotes	ABCG5
T858	4838-4843	PR:000003573	denotes	ABCG8
T859	4998-5003	SO:0000704	denotes	genes
T860	5090-5100	PR:000003572	denotes	sterolin-1
T861	5105-5115	PR:000003573	denotes	sterolin-2
T862	5279-5287	GO:0016020	denotes	membrane
T863	5288-5295	SO:0000417	denotes	domains
T864	5322-5335	SO:0001077	denotes	transmembrane
T865	5327-5335	GO:0016020	denotes	membrane
T866	5485-5495	GO:0010467	denotes	expression
T867	5499-5504	PR:000003572	denotes	ABCG5
T868	5505-5510	PR:000003573	denotes	ABCG8
T869	5527-5532	NCBITaxon:10088	denotes	mouse
T870	5563-5570	UBERON:0002294	denotes	biliary
T871	5571-5582	CHEBI:16113	denotes	cholesterol
T872	5657-5668	CHEBI:16113	denotes	cholesterol
T873	5702-5707	PR:000003572	denotes	ABCG5
T874	5712-5717	PR:000003573	denotes	ABCG8
T875	5775-5781	GO:0045177	denotes	apical
T876	5796-5806	GO:0010467	denotes	expression
T877	5832-5838	GO:0045177	denotes	apical
T878	5899-5904	PR:000003572	denotes	Abcg5
T879	5905-5910	PR:000003573	denotes	Abcg8
T880	5927-5931	NCBITaxon:10088	denotes	mice
T881	6117-6124	CHEBI:15889	denotes	sterols
T882	6235-6240	PR:000003572	denotes	Abcg5
T883	6244-6249	PR:000003573	denotes	Abcg8
T884	6259-6264	NCBITaxon:10088	denotes	mouse
T885	6287-6292	PR:000003572	denotes	Abcg5
T886	6297-6302	PR:000003573	denotes	Abcg8
T887	6364-6369	NCBITaxon:9606	denotes	human
T888	6431-6436	NCBITaxon:9606	denotes	human
T889	6496-6500	SO:0000704	denotes	gene
T890	6707-6714	UBERON:0002294	denotes	biliary
T891	6728-6735	CHEBI:15889	denotes	sterols
T892	6739-6749	UBERON:0000160	denotes	intestinal
T893	6739-6760	GO:0050892	denotes	intestinal absorption
T894	6764-6771	CHEBI:15889	denotes	sterols
T895	6812-6818	NCBITaxon:9606	denotes	humans
T896	6834-6840	UBERON:0000062	denotes	organs
T897	6916-6921	NCBITaxon:10088	denotes	mouse
T898	6949-6956	SO:0000704	denotes	genetic
T899	6969-6974	PR:000003573	denotes	Abcg8
T900	6976-6980	NCBITaxon:10088	denotes	Mice
T901	6996-7001	PR:000003573	denotes	Abcg8
T902	7050-7055	NCBITaxon:9606	denotes	human
T903	7114-7121	UBERON:0002294	denotes	biliary
T904	7122-7128	CHEBI:15889	denotes	sterol
T905	7180-7185	PR:000003573	denotes	Abcg8
T906	7196-7203	NCBITaxon:33208	denotes	animals
T907	7211-7218	GO:0046903	denotes	secrete
T908	7219-7230	CHEBI:16113	denotes	cholesterol
T909	7236-7240	UBERON:0001970	denotes	bile
T910	7251-7261	CHEBI:27693	denotes	sitosterol
T2066	7301-7317	SO:0001644	denotes	Targeting vector
T2067	7358-7362	NCBITaxon:10088	denotes	mice
T2068	7365-7370	NCBITaxon:10088	denotes	mouse
T2069	7371-7378	SO:0001026	denotes	genomic
T2070	7379-7388	NCBITaxon:2	denotes	bacterial
T2071	7379-7410	SO:0000153	denotes	bacterial artificial chromosome
T2072	7412-7415	SO:0000153	denotes	BAC
T2073	7435-7442	CL:0002322	denotes	ES cell
T2074	7523-7530	SO:0000112	denotes	primers
T2075	7562-7567	NCBITaxon:10088	denotes	mouse
T2076	7568-7573	PR:000003572	denotes	Abcg5
T2077	7578-7583	PR:000003573	denotes	Abcg8
T2078	7629-7632	SO:0000153	denotes	BAC
T2079	7673-7680	SO:0001026	denotes	genomic
T2080	7698-7703	PR:000003573	denotes	Abcg8
T2081	7892-7899	SO:0001026	denotes	genomic
T2082	7928-7932	SO:0000147	denotes	exon
T2083	7946-7950	SO:0000147	denotes	exon
T2084	8020-8026	SO:0000440	denotes	vector
T2085	8044-8051	SO:0001026	denotes	genomic
T2086	8081-8085	SO:0000147	denotes	exon
T2087	8099-8103	SO:0000147	denotes	exon
T2088	8204-8210	SO:0000188	denotes	intron
T2089	8222-8226	SO:0000147	denotes	exon
T2090	8241-8245	SO:0000147	denotes	exon
T2091	8267-8275	CHEBI:7507	denotes	neomycin
T2092	8287-8295	SO:0005853	denotes	cassette
T2093	8364-8372	CL:0002322	denotes	ES cells
T2094	8437-8453	SO:0001644	denotes	targeting vector
T2095	8488-8497	UBERON:0000922	denotes	embryonic
T2096	8498-8509	CL:0000057	denotes	fibroblasts
T2097	8511-8522	GO:0009294	denotes	Transfected
T2098	8601-8605	CHEBI:42768	denotes	G418
T2099	8651-8659	CL:0002322	denotes	ES cells
T2100	8719-8733	SO:0000121	denotes	forward primer
T2101	8778-8786	SO:0005853	denotes	cassette
T2102	8792-8806	SO:0000132	denotes	reverse primer
T2103	8807-8813	SO:0000188	denotes	intron
T2104	8899-8904	NCBITaxon:10088	denotes	mouse
T2105	8905-8910	PR:000003573	denotes	Abcg8
T2106	8911-8917	SO:0000188	denotes	intron
T2107	8941-8948	CL:0002322	denotes	ES cell
T2108	8999-9006	CL:0002322	denotes	ES cell
T2109	9023-9028	PR:P23940	denotes	BamHI
T2110	9061-9063	SO:0000028	denotes	bp
T2111	9090-9096	SO:0000188	denotes	intron
T2112	9103-9107	SO:0000147	denotes	exon
T2113	9113-9118	PR:000003573	denotes	Abcg8
T2114	9119-9123	SO:0000704	denotes	gene
T2115	9145-9153	CL:0002322	denotes	ES cells
T2116	9185-9196	UBERON:0000358	denotes	blastocysts
T2117	9267-9271	NCBITaxon:10088	denotes	mice
T2118	9280-9284	UBERON:0010166	denotes	coat
T2119	9358-9362	NCBITaxon:10088	denotes	mice
T2120	9411-9415	NCBITaxon:10088	denotes	mice
T2121	9419-9424	PR:000003573	denotes	Abcg8
T2122	9425-9429	SO:0000704	denotes	gene
T2123	9465-9469	NCBITaxon:10088	denotes	mice
T2124	9500-9504	NCBITaxon:10088	denotes	mice
T2125	9578-9582	NCBITaxon:10088	denotes	mice
T2687	9585-9592	NCBITaxon:33208	denotes	Animals
T2688	9607-9611	NCBITaxon:10088	denotes	mice
T2689	9642-9648	NCBITaxon:9989	denotes	rodent
T2690	9649-9653	CHEBI:33290	denotes	chow
T2691	9669-9674	NCBITaxon:10088	denotes	mouse
T2692	9675-9678	NCBITaxon:10114	denotes	rat
T2693	9717-9728	CHEBI:16113	denotes	cholesterol
T2694	9739-9750	CHEBI:28623	denotes	campesterol
T2695	9766-9776	CHEBI:27693	denotes	sitosterol
T2696	9799-9804	CHEBI:15377	denotes	water
T2697	9868-9875	NCBITaxon:33208	denotes	animals
T2698	9921-9928	NCBITaxon:33208	denotes	animals
T2699	9968-9974	NCBITaxon:33208	denotes	Animal
T2700	10010-10016	NCBITaxon:33208	denotes	Animal
T2851	10139-10144	NCBITaxon:10088	denotes	Mouse
T2852	10145-10149	UBERON:0002415	denotes	tail
T2853	10215-10220	NCBITaxon:10088	denotes	mouse
T2854	10221-10225	UBERON:0002415	denotes	tail
T2855	10253-10258	GO:0019835	denotes	lysis
T2856	10273-10277	CHEBI:9754	denotes	Tris
T2857	10278-10281	CHEBI:17883	denotes	HCl
T2858	10310-10314	CHEBI:26710	denotes	NaCl
T2859	10319-10322	CHEBI:8984	denotes	SDS
T2860	10394-10398	CHEBI:26710	denotes	NaCl
T2861	10423-10431	CHEBI:75958	denotes	solution
T2862	10485-10493	CHEBI:75958	denotes	solution
T2863	10630-10634	CHEBI:16236	denotes	EtOH
T2864	10692-10696	CHEBI:16236	denotes	EtOH
T2865	10832-10839	SO:0001026	denotes	genomic
T2866	10917-10924	SO:0000112	denotes	primers
T2867	10930-10935	PR:000003573	denotes	Abcg8
T2868	10936-10940	SO:0000704	denotes	gene
T2869	10950-10957	SO:0000112	denotes	primers
T2870	11061-11066	PR:000003573	denotes	Abcg8
T2871	11135-11140	CHEBI:6636	denotes	MgCl2
T2872	11148-11157	CHEBI:62946	denotes	(NH4)2SO4
T2873	11179-11183	CHEBI:9754	denotes	Tris
T2874	11184-11187	CHEBI:17883	denotes	HCl
T2875	11210-11216	SO:0000112	denotes	primer
T2876	11218-11223	CHEBI:15377	denotes	water
T2877	11241-11244	NCBITaxon:271	denotes	Taq
T2878	11363-11375	SO:0000006	denotes	PCR products
T3217	11484-11490	PR:P23940	denotes	Bam HI
T3218	11531-11538	CHEBI:2511	denotes	agarose
T3219	11562-11567	CHEBI:25614	denotes	nylon
T3220	11605-11615	GO:0097617	denotes	hybridized
T3221	11627-11629	SO:0000028	denotes	bp
T3222	11656-11662	SO:0000188	denotes	intron
T3223	11669-11673	SO:0000147	denotes	exon
T3224	11679-11684	PR:000003573	denotes	Abcg8
T3225	11685-11688	CHEBI:37972	denotes	32P
T3312	11779-11784	UBERON:0002107	denotes	liver
T3313	11789-11799	UBERON:0000160	denotes	intestinal
T3314	11815-11820	PR:000003573	denotes	Abcg8
T3315	11825-11830	PR:000003573	denotes	Abcg8
T3316	11838-11843	PR:000003573	denotes	Abcg8
T3317	11847-11851	NCBITaxon:10088	denotes	mice
T3318	11938-11943	PR:000003572	denotes	Abcg5
T3319	11953-11955	SO:0000028	denotes	bp
T3320	12022-12027	PR:000003573	denotes	Abcg8
T3321	12037-12039	SO:0000028	denotes	bp
T3322	12041-12043	CHEBI:25613	denotes	nt
T3433	12090-12111	GO:0001171	denotes	Reverse Transcription
T3434	12150-12170	GO:0000380	denotes	alternative splicing
T3435	12188-12194	SO:0001023	denotes	allele
T3436	12236-12241	NCBITaxon:10088	denotes	mouse
T3437	12242-12247	UBERON:0002107	denotes	liver
T3438	12300-12305	NCBITaxon:10088	denotes	mouse
T3439	12306-12312	UBERON:0002107	denotes	livers
T3440	12326-12345	GO:0001171	denotes	reverse transcribed
T3441	12530-12537	SO:0000112	denotes	primers
T3442	12769-12771	SO:0000028	denotes	bp
T3627	12825-12832	SO:0000112	denotes	Primers
T3628	12870-12876	SO:0000112	denotes	primer
T3629	12977-12993	_FRAGMENT	denotes	amplification of
T3630	13028-13031	GO:0006277	denotes	DNA
T3631	13020-13027	SO:0001026	denotes	genomic
T3632	13109-13114	NCBITaxon:10088	denotes	mouse
T3633	13115-13121	UBERON:0002107	denotes	livers
T3634	13135-13154	GO:0001171	denotes	reverse transcribed
T3635	13492-13502	CHEBI:51461	denotes	SYBR Green
T3636	13514-13517	CHEBI:60004	denotes	mix
T3637	13643-13664	GO:0001171	denotes	reverse transcription
T3638	13717-13724	SO:0000112	denotes	primers
T3639	13874-13878	GO:0032508	denotes	melt
T3640	13911-13920	GO:0097617	denotes	annealing
T3641	14128-14140	SO:0000006	denotes	PCR products
T3642	14316-14323	SO:0000673	denotes	message
T3643	14316-14323	CHEBI:33695	denotes	message
T3644	14327-14332	SO:0000704	denotes	genes
T3645	14354-14359	NCBITaxon:10088	denotes	mouse
T3646	14360-14365	UBERON:0002107	denotes	liver
T3647	14678-14682	SO:0000704	denotes	gene
T4184	15101-15107	UBERON:0001969	denotes	Plasma
T4185	15108-15113	CHEBI:18059	denotes	lipid
T4186	15190-15195	UBERON:0000178	denotes	blood
T4187	15229-15234	PR:000003573	denotes	Abcg8
T4188	15239-15244	PR:000003573	denotes	Abcg8
T4189	15252-15257	PR:000003573	denotes	Abcg8
T4190	15261-15265	NCBITaxon:10088	denotes	mice
T4191	15296-15303	UBERON:0001697	denotes	orbital
T4192	15304-15317	UBERON:0001593	denotes	venous plexus
T4193	15358-15368	CHEBI:6015	denotes	isoflurane
T4194	15462-15468	UBERON:0001969	denotes	plasma
T4372	15721-15727	CHEBI:15889	denotes	Sterol
T4373	15743-15749	UBERON:0001969	denotes	plasma
T4374	15754-15761	UBERON:0000479	denotes	tissues
T4375	15804-15808	NCBITaxon:10088	denotes	mice
T4376	15809-15812	GO:0007631	denotes	fed
T4377	15824-15830	NCBITaxon:9989	denotes	rodent
T4378	15831-15835	CHEBI:33290	denotes	chow
T4379	15842-15847	UBERON:0000178	denotes	blood
T4380	15852-15859	UBERON:0000479	denotes	tissues
T4381	15881-15892	CHEBI:6015	denotes	isofluorane
T4382	15921-15926	PR:000003573	denotes	Abcg8
T4383	15931-15936	PR:000003573	denotes	Abcg8
T4384	15944-15949	PR:000003573	denotes	Abcg8
T4385	15953-15957	NCBITaxon:10088	denotes	mice
T4386	15982-15987	UBERON:0000178	denotes	Blood
T4387	16017-16024	UBERON:0001697	denotes	orbital
T4388	16025-16038	UBERON:0001593	denotes	venous plexus
T4389	16070-16077	NCBITaxon:33208	denotes	animals
T4390	16097-16105	UBERON:0005434	denotes	cervical
T4391	16122-16128	UBERON:0000479	denotes	tissue
T4392	16141-16147	UBERON:0001969	denotes	Plasma
T4393	16179-16183	CHEBI:32145	denotes	NaOH
T4394	16205-16210	CHEBI:15377	denotes	water
T4395	16221-16228	CHEBI:15889	denotes	sterols
T4396	16280-16293	CHEBI:27750	denotes	ethyl acetate
T4397	16312-16325	CHEBI:35515	denotes	5α-cholestane
T4398	16369-16374	NCBITaxon:10088	denotes	Mouse
T4399	16375-16382	UBERON:0000479	denotes	tissues
T4400	16384-16389	UBERON:0002107	denotes	liver
T4401	16391-16397	UBERON:0002106	denotes	spleen
T4402	16402-16407	UBERON:0000955	denotes	brain
T4403	16615-16621	CHEBI:15889	denotes	sterol
T4404	16642-16648	UBERON:0000479	denotes	tissue
T4405	16702-16708	CHEBI:15889	denotes	sterol
T4406	16780-16786	CHEBI:29021	denotes	hexane
T4407	17049-17055	UBERON:0001969	denotes	plasma
T4408	17060-17066	UBERON:0000479	denotes	tissue
T4409	17067-17074	CHEBI:15889	denotes	sterols
T4410	17118-17124	UBERON:0000479	denotes	tissue
T4765	17152-17160	GO:0042571	denotes	antibody
T4766	17203-17208	PR:000003572	denotes	Abcg5
T4767	17209-17219	PR:000003572	denotes	sterolin-1
T4768	17220-17230	GO:0042571	denotes	antibodies
T4769	17305-17310	PR:000003572	denotes	Abcg5
T4770	17311-17321	PR:000003572	denotes	sterolin-1
T4771	17322-17332	GO:0042571	denotes	antibodies
T4772	17410-17415	UBERON:0002107	denotes	Liver
T4874	17467-17475	GO:0016020	denotes	Membrane
T4875	17503-17512	GO:0016020	denotes	membranes
T4876	17561-17566	UBERON:0002107	denotes	liver
T4877	17567-17573	UBERON:0000479	denotes	tissue
T4878	17624-17629	GO:0019835	denotes	lysis
T4879	17643-17647	CHEBI:9754	denotes	Tris
T4880	17662-17669	CHEBI:17992	denotes	sucrose
T4881	18236-18241	GO:0019835	denotes	lysis
T4882	18250-18266	GO:0005886	denotes	Plasma membranes
T4883	18465-18473	GO:0016020	denotes	membrane
T4884	18510-18513	CHEBI:8984	denotes	SDS
T4885	18514-18524	CHEBI:28619	denotes	acrylamide
T5125	18567-18570	CHEBI:8984	denotes	SDS
T5126	18596-18610	CHEBI:53325	denotes	nitrocellulose
T5127	18723-18731	CHEBI:53424	denotes	Tween 20
T5128	18770-18778	GO:0042571	denotes	antibody
T5129	18787-18792	PR:000003572	denotes	Abcg5
T5130	18811-18812	CHEBI:53424	denotes	T
T5131	18892-18893	CHEBI:53424	denotes	T
T5132	18895-18899	CHEBI:9754	denotes	Tris
T5133	18921-18929	CHEBI:53424	denotes	Tween-20
T5134	18943-18947	CHEBI:26710	denotes	NaCl
T5135	18978-18982	NCBITaxon:9925	denotes	goat
T5136	18988-18994	NCBITaxon:9986	denotes	rabbit
T5137	18995-19005	MOP:0000779	denotes	conjugated
T5138	19046-19047	CHEBI:53424	denotes	T
T5139	19124-19125	CHEBI:53424	denotes	T
T5140	19138-19142	CHEBI:26710	denotes	NaCl
T5141	19196-19203	CHEBI:33893	denotes	Reagent
T5355	19353-19358	UBERON:0002107	denotes	liver
T5356	19363-19372	UBERON:0000160	denotes	intestine
T5357	19373-19379	UBERON:0000479	denotes	tissue
T5358	19536-19547	CHEBI:51686	denotes	hematoxylin
T5359	19607-19615	CHEBI:17790	denotes	methanol
T5360	19674-19682	GO:0042571	denotes	antibody
T5361	19740-19744	NCBITaxon:9925	denotes	goat
T5362	19745-19750	UBERON:0001977	denotes	serum
T5363	19807-19817	PR:000003572	denotes	sterolin-1
T5364	19818-19826	GO:0042571	denotes	antibody
T5365	19926-19934	GO:0042571	denotes	antibody
T5366	19936-19940	NCBITaxon:9925	denotes	goat
T5367	19946-19952	NCBITaxon:9986	denotes	rabbit
T5368	19953-19956	GO:0071735	denotes	IgG
T5369	19957-19967	MOP:0000779	denotes	conjugated
T5370	20147-20152	UBERON:0002107	denotes	Liver
T5371	20179-20186	CHEBI:15347	denotes	acetone
T5372	20232-20240	CHEBI:75958	denotes	solution
T5373	20254-20262	CHEBI:53424	denotes	Tween-20
T5374	20266-20270	NCBITaxon:9925	denotes	goat
T5375	20271-20276	UBERON:0001977	denotes	serum
T5376	20321-20331	PR:000003572	denotes	sterolin-1
T5377	20357-20361	PR:000003549	denotes	Bsep
T5378	20394-20399	CHEBI:53422	denotes	Tween
T5379	20467-20472	CHEBI:53422	denotes	Tween
T5380	20491-20499	GO:0042571	denotes	antibody
T5381	20501-20505	NCBITaxon:9925	denotes	goat
T5382	20511-20517	NCBITaxon:9986	denotes	rabbit
T5383	20518-20527	CHEBI:52661	denotes	Alexa 488
T5384	20663-20667	CHEBI:51231	denotes	Dapi
T5385	20674-20682	CHEBI:75958	denotes	solution
T5386	20795-20799	CHEBI:51231	denotes	Dapi
T5387	20854-20859	PR:000003572	denotes	Abcg5
T5388	20864-20868	PR:000003549	denotes	Bsep
T5831	21003-21015	GO:0009294	denotes	Transfection
T5832	21079-21090	GO:0009294	denotes	transfected
T5833	21101-21106	NCBITaxon:10088	denotes	mouse
T5834	21107-21112	PR:000003572	denotes	Abcg5
T5835	21122-21127	NCBITaxon:10088	denotes	mouse
T5836	21128-21133	PR:000003573	denotes	Abcg8
T5837	21134-21144	GO:0010467	denotes	expression
T5838	21183-21190	CHEBI:33893	denotes	reagent
T5839	21297-21309	GO:0009294	denotes	transfection
T5840	21342-21347	GO:0040007	denotes	grown
T5841	21363-21366	CHEBI:16526	denotes	CO2
T5842	21411-21421	CHEBI:17334	denotes	penicillin
T5843	21433-21445	CHEBI:17076	denotes	streptomycin
T5844	21479-21491	GO:0009294	denotes	transfection
T5845	21537-21544	CHEBI:33893	denotes	reagent
T5846	21580-21587	CHEBI:60004	denotes	mixture
T5847	21744-21755	GO:0009294	denotes	transfected
T5848	21805-21815	GO:0010467	denotes	expression
T5849	21850-21857	CHEBI:60004	denotes	mixture
T5850	21861-21868	CHEBI:15347	denotes	acetone
T5851	21869-21877	CHEBI:17790	denotes	methanol
T5852	21941-21945	NCBITaxon:9925	denotes	goat
T5853	21946-21951	UBERON:0001977	denotes	serum
T5854	22020-22028	GO:0042571	denotes	antibody
T5855	22034-22039	PR:000003572	denotes	Abcg5
T5856	22046-22050	NCBITaxon:9925	denotes	goat
T5857	22051-22056	UBERON:0001977	denotes	serum
T5858	22133-22139	NCBITaxon:9986	denotes	rabbit
T5859	22140-22152	GO:0071736	denotes	IgG antibody
T6175	22260-22267	UBERON:0002294	denotes	biliary
T6176	22268-22273	CHEBI:18059	denotes	lipid
T6177	22298-22302	NCBITaxon:10088	denotes	Mice
T6178	22330-22340	UBERON:0001179	denotes	peritoneal
T6179	22362-22370	CHEBI:119915	denotes	fentanyl
T6180	22395-22403	CHEBI:49575	denotes	Diazepam
T6181	22409-22416	UBERON:0000916	denotes	abdomen
T6182	22462-22471	UBERON:0002394	denotes	bile duct
T6183	22477-22488	UBERON:0002110	denotes	gallbladder
T6184	22505-22509	UBERON:0001970	denotes	Bile
T6185	22584-22588	UBERON:0001970	denotes	Bile
T6186	22626-22633	UBERON:0002294	denotes	Biliary
T6187	22634-22638	UBERON:0001970	denotes	bile
T6188	22634-22643	CHEBI:22868	denotes	bile salt
T6189	22645-22651	CHEBI:15889	denotes	sterol
T6190	22656-22668	CHEBI:16247	denotes	phospholipid
T6191	22770-22777	UBERON:0002294	denotes	biliary
T6192	22778-22783	CHEBI:18059	denotes	lipid
T6193	22795-22799	UBERON:0001970	denotes	bile
T6194	22850-22854	UBERON:0001970	denotes	bile
T6195	22850-22859	CHEBI:22868	denotes	bile salt
T6196	22970-22982	UBERON:0004711	denotes	jugular vein
T6197	23055-23059	UBERON:0001970	denotes	Bile
T6198	23111-23115	UBERON:0001970	denotes	bile
T6199	23111-23120	CHEBI:22868	denotes	bile salt
T6200	23125-23130	CHEBI:18059	denotes	lipid
T6201	23198-23207	UBERON:0002394	denotes	bile duct
T6202	23227-23238	UBERON:0002110	denotes	gallbladder
T6203	23240-23244	UBERON:0001970	denotes	bile
T6204	23279-23283	NCBITaxon:10088	denotes	mice
T6205	23333-23337	NCBITaxon:10088	denotes	mice
T6206	23471-23477	CHEBI:15889	denotes	Sterol
T6207	23490-23494	UBERON:0001970	denotes	bile
T6576	23554-23561	CHEBI:11814	denotes	HMG-CoA
T6577	23566-23577	CHEBI:16113	denotes	cholesterol
T6578	23609-23614	NCBITaxon:10088	denotes	Mouse
T6579	23615-23620	UBERON:0002107	denotes	liver
T6580	23702-23707	UBERON:0002107	denotes	liver
T6581	23792-23799	CHEBI:17992	denotes	sucrose
T6582	23807-23811	CHEBI:9754	denotes	Tris
T6583	23820-23833	CHEBI:64734	denotes	disodium EDTA
T6584	23842-23845	CHEBI:18320	denotes	DTT
T6585	24002-24023	CHEBI:32031	denotes	dipotassium phosphate
T6586	24032-24035	CHEBI:32588	denotes	KCl
T6587	24042-24045	CHEBI:18320	denotes	DTT
T6588	24052-24065	CHEBI:64734	denotes	disodium EDTA
T6589	24071-24079	CHEBI:17754	denotes	glycerol
T6590	24214-24225	CHEBI:16113	denotes	cholesterol
T6591	24375-24382	CHEBI:60004	denotes	mixture
T6592	24453-24459	CHEBI:32031	denotes	K2HPO4
T6593	24479-24482	CHEBI:18320	denotes	DTT
T6594	24490-24502	CHEBI:17154	denotes	nicotinamide
T6595	24514-24517	CHEBI:36927	denotes	14C
T6596	24519-24530	CHEBI:16113	denotes	cholesterol
T6597	24582-24596	CHEBI:495055	denotes	β-cyclodextrin
T6598	24720-24736	GO:0006741	denotes	NADPH-generating
T6599	24752-24757	CHEBI:18009	denotes	NADP+
T6600	24765-24784	CHEBI:14314	denotes	glucose-6-phosphate
T6601	24795-24814	CHEBI:14314	denotes	glucose-6-phosphate
T6602	24908-24911	CHEBI:32035	denotes	KOH
T6603	24921-24930	MOP:0000370	denotes	butylated
T6604	24931-24945	CHEBI:24751	denotes	hydroxytoluene
T6605	24960-24969	CHEBI:16236	denotes	ethanolic
T6606	24970-24989	CHEBI:32035	denotes	potassium hydroxide
T6607	24992-24994	CHEBI:29238	denotes	3H
T6608	24996-25017	CHEBI:17500	denotes	7α-hydroxycholesterol
T6609	25124-25131	CHEBI:15889	denotes	sterols
T6610	25166-25174	CHEBI:29021	denotes	n-hexane
T6611	25274-25282	CHEBI:29021	denotes	n-hexane
T6612	25283-25293	CHEBI:17824	denotes	2-propanol
T6613	25326-25332	CHEBI:30563	denotes	silica
T6614	25369-25377	CHEBI:29021	denotes	n-hexane
T6615	25378-25388	CHEBI:17824	denotes	2-propanol
T6616	25406-25427	CHEBI:17500	denotes	7α-hydroxycholesterol
T6617	25452-25460	CHEBI:29021	denotes	n-hexane
T6618	25461-25471	CHEBI:17824	denotes	2-propanol
T6619	25519-25525	CHEBI:30563	denotes	silica
T6620	25538-25544	CHEBI:30563	denotes	Silica
T6621	25591-25604	CHEBI:35702	denotes	diethyl ether
T6622	25783-25790	CHEBI:11814	denotes	HMG-CoA
T7246	26081-26088	NCBITaxon:33208	denotes	animals
T7332	26153-26158	PR:000003573	denotes	Abcg8
T7333	26159-26169	PR:000003573	denotes	sterolin-2
T7334	26180-26184	NCBITaxon:10088	denotes	mice
T7335	26267-26275	GO:0008380	denotes	splicing
T7336	26328-26333	SO:0000147	denotes	exons
T7337	26349-26356	CL:0002322	denotes	ES cell
T7338	26402-26409	SO:0000155	denotes	plasmid
T7339	26442-26444	CL:0002322	denotes	ES
T7340	26463-26465	CL:0002322	denotes	ES
T7341	26500-26510	UBERON:0000358	denotes	Blastocyst
T7342	26563-26567	NCBITaxon:10088	denotes	mice
T7343	26640-26642	CL:0002322	denotes	ES
T7344	26795-26799	NCBITaxon:10088	denotes	mice
T7345	26873-26880	NCBITaxon:33208	denotes	animals
T7346	26936-26940	NCBITaxon:10088	denotes	mice
T7347	27074-27078	NCBITaxon:10088	denotes	mice
T7348	27109-27114	PR:000003573	denotes	Abcg8
T7349	27116-27126	GO:0010467	denotes	expression
T7350	27130-27135	PR:000003572	denotes	Abcg5
T7351	27198-27218	GO:0000380	denotes	alternative splicing
T7352	27228-27241	_FRAGMENT	denotes	production of
T7353	27290-27297	GO:0006412	denotes	protein
T7354	27273-27278	PR:000003573	denotes	Abcg8
T7355	27279-27289	PR:000003573	denotes	sterolin-2
T7356	27332-27337	PR:000003572	denotes	Abcg5
T7357	27338-27348	PR:000003572	denotes	sterolin-1
T7358	27379-27398	GO:0001171	denotes	reverse transcribed
T7359	27410-27415	UBERON:0002107	denotes	liver
T7360	27425-27430	PR:000003573	denotes	Abcg8
T7361	27441-27445	NCBITaxon:10088	denotes	mice
T7362	27459-27464	PR:000003573	denotes	Abcg8
T7363	27465-27475	PR:000003573	denotes	sterolin-2
T7364	27528-27532	SO:0000147	denotes	exon
T7365	27542-27547	PR:000003573	denotes	Abcg8
T7366	27558-27562	NCBITaxon:10088	denotes	mice
T7367	27572-27579	SO:0000112	denotes	primers
T7368	27596-27601	SO:0000147	denotes	exons
T7369	27612-27617	SO:0000147	denotes	exons
T7370	27630-27635	SO:0000147	denotes	exons
T7371	27691-27712	GO:0000380	denotes	alternatively spliced
T7372	27691-27720	SO:1001187	denotes	alternatively spliced message
T7373	27713-27720	CHEBI:33695	denotes	message
T7374	27797-27802	PR:000003573	denotes	Abcg8
T7375	27813-27817	NCBITaxon:10088	denotes	mice
T7376	27823-27831	GO:0016020	denotes	membrane
T7377	27841-27848	SO:0000417	denotes	domains
T7378	27852-27857	PR:000003573	denotes	Abcg8
T7379	27858-27868	PR:000003573	denotes	sterolin-2
T7380	27884-27889	SO:0000147	denotes	exons
T7883	27897-27903	CHEBI:15889	denotes	Sterol
T7884	27914-27919	PR:000003573	denotes	Abcg8
T7885	27920-27930	PR:000003573	denotes	sterolin-2
T7886	27941-27948	NCBITaxon:33208	denotes	animals
T7887	27958-27963	NCBITaxon:3193	denotes	plant
T7888	27964-27971	CHEBI:15889	denotes	sterols
T7889	27975-27981	UBERON:0000479	denotes	tissue
T7890	27986-27992	UBERON:0001969	denotes	plasma
T7891	28027-28033	CHEBI:15889	denotes	Sterol
T7892	28096-28102	UBERON:0001969	denotes	plasma
T7893	28107-28114	UBERON:0000479	denotes	tissues
T7894	28122-28127	PR:000003573	denotes	Abcg8
T7895	28131-28135	NCBITaxon:10088	denotes	mice
T7896	28187-28193	UBERON:0000479	denotes	tissue
T7897	28251-28257	UBERON:0001969	denotes	Plasma
T7898	28258-28269	CHEBI:16113	denotes	cholesterol
T7899	28312-28316	NCBITaxon:10088	denotes	mice
T7900	28393-28397	NCBITaxon:10088	denotes	mice
T7901	28427-28432	NCBITaxon:3193	denotes	Plant
T7902	28433-28440	CHEBI:15889	denotes	sterols
T7903	28496-28500	NCBITaxon:10088	denotes	mice
T7904	28537-28542	PR:000003573	denotes	Abcg8
T7905	28546-28550	NCBITaxon:10088	denotes	mice
T7906	28552-28558	UBERON:0001969	denotes	Plasma
T7907	28559-28570	CHEBI:28623	denotes	campesterol
T7908	28575-28585	CHEBI:27693	denotes	sitosterol
T7909	28627-28632	PR:000003573	denotes	Abcg8
T7910	28636-28640	NCBITaxon:10088	denotes	mice
T7911	28663-28667	NCBITaxon:10088	denotes	mice
T7912	28805-28811	UBERON:0001969	denotes	plasma
T7913	28812-28817	NCBITaxon:3193	denotes	plant
T7914	28818-28824	CHEBI:15889	denotes	sterol
T7915	28867-28871	NCBITaxon:10088	denotes	mice
T7916	28873-28878	UBERON:0002107	denotes	Liver
T7917	28879-28890	CHEBI:16113	denotes	cholesterol
T7918	28906-28911	PR:000003573	denotes	Abcg8
T7919	28915-28919	NCBITaxon:10088	denotes	mice
T7920	28962-28967	UBERON:0002107	denotes	liver
T7921	28996-29002	UBERON:0000479	denotes	tissue
T7922	29038-29044	UBERON:0000479	denotes	tissue
T7923	29073-29084	CHEBI:16113	denotes	cholesterol
T7924	29131-29142	CHEBI:28623	denotes	campesterol
T7925	29169-29179	CHEBI:27693	denotes	sitosterol
T7926	29227-29233	UBERON:0000479	denotes	tissue
T7927	29252-29257	PR:000003573	denotes	Abcg8
T7928	29270-29274	NCBITaxon:10088	denotes	mice
T7929	29297-29301	NCBITaxon:10088	denotes	mice
T7930	29346-29352	UBERON:0000479	denotes	tissue
T7931	29419-29424	UBERON:0002107	denotes	liver
T7932	29425-29436	CHEBI:16113	denotes	cholesterol
T7933	29440-29445	NCBITaxon:3193	denotes	plant
T7934	29446-29452	CHEBI:15889	denotes	sterol
T7935	29470-29475	PR:000003573	denotes	Abcg8
T7936	29483-29488	PR:000003573	denotes	Abcg8
T7937	29492-29496	NCBITaxon:10088	denotes	mice
T7938	29498-29504	UBERON:0002106	denotes	Spleen
T7939	29505-29511	CHEBI:15889	denotes	sterol
T7940	29535-29540	UBERON:0002107	denotes	liver
T7941	29591-29596	UBERON:0000955	denotes	brain
T7942	29597-29603	CHEBI:15889	denotes	sterol
T7943	29660-29679	UBERON:0000120	denotes	blood brain barrier
T7944	29703-29711	_FRAGMENT	denotes	entry of
T7945	29718-29725	GO:0035376	denotes	sterols
T7946	29718-29725	CHEBI:15889	denotes	sterols
T7947	29781-29786	NCBITaxon:3193	denotes	plant
T7948	29787-29794	CHEBI:15889	denotes	sterols
T7949	29802-29808	UBERON:0000955	denotes	brains
T7950	29823-29830	NCBITaxon:33208	denotes	animals
T7951	29843-29848	UBERON:0000178	denotes	blood
T7952	29870-29876	UBERON:0000479	denotes	tissue
T7953	29933-29939	UBERON:0000479	denotes	tissue
T7954	29940-29945	NCBITaxon:3193	denotes	plant
T7955	29946-29953	CHEBI:15889	denotes	sterols
T7956	29976-29982	UBERON:0002107	denotes	livers
T7957	29986-29991	PR:000003573	denotes	Abcg8
T7958	30008-30012	NCBITaxon:10088	denotes	mice
T7959	30047-30069	CHEBI:17002	denotes	esterified cholesterol
T7960	30133-30143	CHEBI:27693	denotes	sitosterol
T7961	30147-30158	CHEBI:28623	denotes	campesterol
T7962	30246-30252	UBERON:0000479	denotes	tissue
T7963	30253-30259	CHEBI:15889	denotes	sterol
T7964	30278-30285	CHEBI:15889	denotes	sterols
T7965	30305-30312	CHEBI:15889	denotes	sterols
T7966	30363-30370	CHEBI:15889	denotes	sterols
T7967	30376-30389	CHEBI:17855	denotes	triglycerides
T7968	30408-30414	UBERON:0001969	denotes	plasma
T7969	30435-30442	NCBITaxon:33208	denotes	animals
T7970	30443-30446	GO:0007631	denotes	fed
T7971	30457-30461	CHEBI:33290	denotes	chow
T7972	30517-30523	CHEBI:15889	denotes	sterol
T7973	30568-30573	PR:000003573	denotes	Abcg8
T7974	30586-30590	NCBITaxon:10088	denotes	mice
T7975	30618-30630	CHEBI:17855	denotes	triglyceride
T7976	30709-30713	NCBITaxon:10088	denotes	mice
T7977	30841-30848	NCBITaxon:33208	denotes	animals
T7978	30856-30862	UBERON:0001969	denotes	plasma
T7979	30863-30875	CHEBI:17855	denotes	triglyceride
T7980	30890-30895	PR:000003573	denotes	Abcg8
T7981	30899-30903	NCBITaxon:10088	denotes	mice
T7982	31149-31164	CHEBI:39015	denotes	apolipoproteins
T7983	31149-31166	PR:000004145	denotes	apolipoproteins B
T7984	31149-31164	_FRAGMENT	denotes	apolipoproteins
T7985	31168-31169	PR:000004155	denotes	E
T7986	31171-31173	PR:000004140	denotes	AI
T7987	31178-31181	PR:000004142	denotes	AII
T7988	31208-31220	CHEBI:17855	denotes	triglyceride
T8918	31260-31265	UBERON:0002107	denotes	Liver
T8919	31266-31270	SO:0000704	denotes	gene
T8920	31266-31281	GO:0010467	denotes	gene expression
T8921	31312-31317	PR:000003573	denotes	Abcg8
T8922	31318-31328	PR:000003573	denotes	sterolin-2
T8923	31339-31343	NCBITaxon:10088	denotes	mice
T8924	31390-31395	PR:000003573	denotes	Abcg8
T8925	31396-31406	PR:000003573	denotes	sterolin-2
T8926	31414-31419	SO:0000704	denotes	genes
T8927	31425-31433	GO:0065007	denotes	regulate
T8928	31434-31440	CHEBI:15889	denotes	sterol
T8929	31434-31451	GO:0016125	denotes	sterol metabolism
T8930	31502-31512	GO:0010467	denotes	expression
T8931	31523-31528	PR:000003572	denotes	Abcg5
T8932	31530-31535	PR:000003573	denotes	Abcg8
T8933	31543-31549	PR:000006148	denotes	Cyp7a1
T8934	31551-31556	PR:000003537	denotes	Abca1
T8935	31558-31562	PR:P21440	denotes	Mdr2
T8936	31569-31574	PR:000028988	denotes	Srebp
T8937	31583-31590	PR:000015612	denotes	Srebp-2
T8938	31603-31609	UBERON:0002107	denotes	livers
T8939	31613-31617	NCBITaxon:10088	denotes	mice
T8940	31618-31621	GO:0007631	denotes	fed
T8941	31632-31636	CHEBI:33290	denotes	chow
T8942	31668-31673	PR:000003573	denotes	Abcg8
T8943	31679-31689	GO:0010467	denotes	expression
T8944	31722-31727	PR:000003573	denotes	Abcg8
T8945	31731-31735	NCBITaxon:10088	denotes	mice
T8946	31781-31785	NCBITaxon:10088	denotes	mice
T8947	31809-31813	NCBITaxon:10088	denotes	mice
T8948	31863-31873	GO:0010467	denotes	expression
T8949	31877-31882	PR:000003572	denotes	Abcg5
T8950	31900-31904	NCBITaxon:10088	denotes	mice
T8951	31965-31969	NCBITaxon:10088	denotes	mice
T8952	32022-32026	NCBITaxon:10088	denotes	mice
T8953	32028-32038	GO:0010467	denotes	Expression
T8954	32042-32049	CHEBI:11814	denotes	HMG-CoA
T8955	32129-32133	NCBITaxon:10088	denotes	mice
T8956	32188-32193	NCBITaxon:9606	denotes	human
T8957	32317-32322	UBERON:0002107	denotes	liver
T8958	32349-32356	CHEBI:11814	denotes	HMG-CoA
T8959	32380-32386	PR:000006148	denotes	Cyp7a1
T8960	32409-32416	CHEBI:11814	denotes	HMG-CoA
T8961	32470-32475	PR:000003573	denotes	Abcg8
T8962	32483-32488	PR:000003573	denotes	Abcg8
T8963	32492-32496	NCBITaxon:10088	denotes	mice
T8964	32563-32573	GO:0010467	denotes	expression
T8965	32601-32607	PR:000006148	denotes	Cyp7a1
T8966	32613-32623	GO:0010467	denotes	expression
T8967	32674-32679	NCBITaxon:10088	denotes	mouse
T8968	32681-32687	PR:000006148	denotes	Cyp7a1
T8969	32764-32768	NCBITaxon:10088	denotes	mice
T8970	32800-32806	PR:000006148	denotes	Cyp7a1
T8971	32823-32833	CHEBI:27693	denotes	Sitosterol
T8972	32870-32879	CHEBI:35222	denotes	inhibitor
T8973	32883-32889	PR:000006148	denotes	Cyp7a1
T8974	32925-32930	NCBITaxon:3193	denotes	plant
T8975	32931-32938	CHEBI:15889	denotes	sterols
T8976	32946-32951	UBERON:0002107	denotes	liver
T8977	33003-33008	NCBITaxon:10088	denotes	mouse
T10840	39532-39539	GO:0046903	denotes	secrete
T9570	33032-33037	PR:000003572	denotes	Abcg5
T9571	33049-33054	PR:000003573	denotes	Abcg8
T9572	33067-33071	NCBITaxon:10088	denotes	mice
T9573	33089-33094	PR:000003573	denotes	Abcg8
T9574	33095-33105	PR:000003573	denotes	sterolin-2
T9575	33124-33129	PR:000003572	denotes	Abcg5
T9576	33130-33140	PR:000003572	denotes	sterolin-1
T9577	33141-33151	GO:0010467	denotes	expression
T9578	33184-33191	SO:0000704	denotes	genetic
T9579	33248-33258	GO:0010467	denotes	expression
T9580	33277-33285	GO:0042571	denotes	antibody
T9581	33289-33294	NCBITaxon:10088	denotes	mouse
T9582	33295-33300	PR:000003573	denotes	Abcg8
T9583	33301-33311	PR:000003573	denotes	sterolin-2
T9584	33386-33392	NCBITaxon:9986	denotes	rabbit
T9585	33404-33412	GO:0042571	denotes	antibody
T9586	33416-33421	NCBITaxon:10088	denotes	mouse
T9587	33422-33427	PR:000003572	denotes	Abcg5
T9588	33428-33438	PR:000003572	denotes	sterolin-1
T9589	33452-33462	GO:0042571	denotes	antibodies
T9590	33537-33542	UBERON:0002107	denotes	liver
T9591	33547-33556	UBERON:0000160	denotes	intestine
T9592	33560-33565	PR:000003573	denotes	Abcg8
T9593	33569-33573	NCBITaxon:10088	denotes	mice
T9594	33595-33600	UBERON:0002107	denotes	liver
T9595	33607-33615	GO:0016020	denotes	membrane
T9596	33671-33676	PR:000003572	denotes	Abcg5
T9597	33677-33687	PR:000003572	denotes	sterolin-1
T9598	33688-33698	GO:0042571	denotes	antibodies
T9599	33759-33764	PR:000003572	denotes	Abcg5
T9600	33765-33775	PR:000003572	denotes	sterolin-1
T9601	33912-33917	PR:000003572	denotes	Abcg5
T9602	33918-33928	PR:000003572	denotes	sterolin-1
T9603	33937-33945	GO:0042571	denotes	antibody
T9604	33991-33999	GO:0042571	denotes	antibody
T9605	34071-34078	NCBITaxon:33208	denotes	animals
T9606	34122-34128	PR:000007078	denotes	Endo-H
T9607	34190-34201	PR:000016261	denotes	transferrin
T9608	34212-34220	GO:0042571	denotes	antibody
T9609	34250-34255	PR:000003572	denotes	Abcg5
T9610	34256-34266	PR:000003572	denotes	sterolin-1
T9611	34280-34287	NCBITaxon:33208	denotes	animals
T9612	34373-34381	GO:0042571	denotes	antibody
T9613	34407-34413	PR:000007078	denotes	Endo-H
T9614	34418-34424	PR:000011196	denotes	PNGase
T9615	34459-34467	GO:0042571	denotes	antibody
T9616	34513-34518	PR:000003572	denotes	Abcg5
T9617	34519-34529	PR:000003572	denotes	sterolin-1
T9618	34543-34550	NCBITaxon:33208	denotes	animals
T9619	34588-34593	NCBITaxon:10088	denotes	mouse
T9620	34635-34645	GO:0042571	denotes	antibodies
T9621	34659-34664	PR:000003572	denotes	Abcg5
T9622	34665-34675	PR:000003572	denotes	sterolin-1
T9623	34777-34787	GO:0042571	denotes	antibodies
T9624	34798-34810	GO:0009294	denotes	transfection
T9625	34827-34835	GO:0042571	denotes	antibody
T9626	34852-34861	GO:0010467	denotes	expressed
T9627	34862-34867	NCBITaxon:10088	denotes	mouse
T9628	34868-34873	PR:000003572	denotes	Abcg5
T9629	34874-34884	PR:000003572	denotes	sterolin-1
T9630	34909-34914	NCBITaxon:10088	denotes	mouse
T9631	34915-34920	PR:000003573	denotes	Abcg8
T9632	34921-34931	PR:000003573	denotes	sterolin-2
T9633	34951-34963	GO:0009294	denotes	transfection
T9634	34989-34994	PR:000003572	denotes	Abcg5
T9635	34999-35004	PR:000003573	denotes	Abcg8
T9636	35109-35119	GO:0010467	denotes	expression
T9637	35123-35128	PR:000003573	denotes	Abcg8
T9638	35138-35148	GO:0010467	denotes	expressing
T9639	35149-35154	PR:000003572	denotes	Abcg5
T9640	35155-35165	PR:000003572	denotes	sterolin-1
T9641	35190-35198	GO:0042571	denotes	antibody
T9642	35203-35208	PR:000003573	denotes	Abcg8
T9643	35209-35219	PR:000003573	denotes	sterolin-2
T9644	35285-35293	GO:0042571	denotes	antibody
T9645	35308-35313	PR:000003572	denotes	Abcg5
T9646	35314-35324	PR:000003572	denotes	sterolin-1
T9647	35355-35360	PR:000003573	denotes	Abcg8
T9648	35361-35371	PR:000003573	denotes	sterolin-2
T9649	35392-35402	UBERON:0000160	denotes	intestinal
T9650	35403-35409	UBERON:0000479	denotes	tissue
T9651	35435-35440	PR:000003572	denotes	Abcg5
T9652	35441-35451	PR:000003572	denotes	sterolin-1
T9653	35490-35495	PR:000003572	denotes	Abcg5
T9654	35496-35506	PR:000003572	denotes	sterolin-1
T9655	35535-35540	PR:000003572	denotes	Abcg5
T9656	35541-35551	PR:000003572	denotes	sterolin-1
T9657	35559-35569	UBERON:0000160	denotes	intestinal
T9658	35590-35596	GO:0045177	denotes	apical
T9659	35631-35642	CL:0000584	denotes	enterocytes
T9660	35670-35675	PR:000003573	denotes	Abcg8
T9661	35676-35686	PR:000003573	denotes	sterolin-2
T9662	35765-35773	GO:0042571	denotes	antibody
T9663	35790-35795	PR:000003572	denotes	Abcg5
T9664	35796-35806	PR:000003572	denotes	sterolin-1
T9665	35839-35847	GO:0042571	denotes	antibody
T9666	35942-35947	PR:000003572	denotes	Abcg5
T9667	35948-35958	PR:000003572	denotes	sterolin-1
T9668	35959-35969	GO:0010467	denotes	expression
T9669	35994-36000	GO:0045177	denotes	apical
T9670	36064-36072	GO:0042571	denotes	antibody
T9671	36082-36087	UBERON:0002107	denotes	liver
T9672	36125-36129	NCBITaxon:10088	denotes	mice
T9673	36154-36164	GO:0010467	denotes	expression
T9674	36168-36174	PR:000003549	denotes	Abcb11
T9675	36237-36241	UBERON:0001970	denotes	bile
T9676	36237-36247	CHEBI:22868	denotes	bile salts
T9677	36273-36278	PR:000003572	denotes	Abcg5
T9678	36279-36289	PR:000003572	denotes	sterolin-1
T9679	36331-36339	GO:0045177	denotes	apically
T9680	36353-36358	UBERON:0002107	denotes	liver
T9681	36383-36387	NCBITaxon:10088	denotes	mice
T9682	36435-36440	UBERON:0002107	denotes	liver
T9683	36455-36459	NCBITaxon:10088	denotes	mice
T9684	36474-36484	GO:0010467	denotes	expression
T9685	36494-36499	PR:000003572	denotes	Abcg5
T9686	36500-36510	PR:000003572	denotes	sterolin-1
T9687	36588-36594	GO:0045177	denotes	apical
T9688	36595-36605	GO:0010467	denotes	expression
T9689	36609-36614	PR:000003572	denotes	Abcg5
T9690	36615-36625	PR:000003572	denotes	sterolin-1
T9691	36642-36646	NCBITaxon:10088	denotes	mice
T9692	36657-36665	GO:0042571	denotes	antibody
T9693	36684-36689	UBERON:0002107	denotes	liver
T9694	36725-36729	NCBITaxon:10088	denotes	mice
T9695	36747-36753	GO:0045177	denotes	apical
T9696	36754-36764	GO:0010467	denotes	expression
T9697	36768-36773	PR:000003572	denotes	Abcg5
T9698	36774-36784	PR:000003572	denotes	sterolin-1
T9699	36816-36822	UBERON:0002107	denotes	livers
T10754	36840-36847	UBERON:0002294	denotes	Biliary
T10755	36861-36865	UBERON:0001970	denotes	bile
T10756	36861-36871	CHEBI:22868	denotes	bile salts
T10757	36873-36879	CHEBI:15889	denotes	sterol
T10758	36884-36897	CHEBI:16247	denotes	phospholipids
T10759	36901-36906	PR:000003573	denotes	Abcg8
T10760	36907-36917	PR:000003573	denotes	sterolin-2
T10761	36928-36932	NCBITaxon:10088	denotes	mice
T10762	36948-36959	NCBITaxon:1	denotes	individuals
T10763	36988-36995	GO:0046903	denotes	secrete
T10764	36996-37007	CHEBI:16113	denotes	cholesterol
T10765	37012-37017	NCBITaxon:3193	denotes	plant
T10766	37018-37025	CHEBI:15889	denotes	sterols
T10767	37031-37035	UBERON:0001970	denotes	bile
T10768	37070-37074	NCBITaxon:10088	denotes	mice
T10769	37098-37105	UBERON:0002294	denotes	biliary
T10770	37106-37112	CHEBI:15889	denotes	sterol
T10771	37153-37157	UBERON:0001970	denotes	bile
T10772	37184-37190	CHEBI:15889	denotes	sterol
T10773	37195-37207	CHEBI:16247	denotes	phospholipid
T10774	37220-37225	PR:000003573	denotes	Abcg8
T10775	37229-37233	NCBITaxon:10088	denotes	mice
T10776	37272-37276	NCBITaxon:10088	denotes	mice
T10777	37296-37300	UBERON:0001970	denotes	bile
T10778	37296-37305	CHEBI:22868	denotes	bile salt
T10779	37351-37357	CHEBI:15889	denotes	sterol
T10780	37385-37397	CHEBI:16247	denotes	phospholipid
T10781	37456-37463	UBERON:0002294	denotes	biliary
T10782	37464-37470	CHEBI:15889	denotes	sterol
T10783	37518-37525	UBERON:0002294	denotes	biliary
T10784	37526-37532	CHEBI:15889	denotes	sterol
T10785	37555-37559	NCBITaxon:10088	denotes	mice
T10786	37594-37619	CHEBI:80774	denotes	tauroursodeoxycholic acid
T10787	37621-37625	CHEBI:80774	denotes	TUDC
T10788	37628-37632	NCBITaxon:10088	denotes	Mice
T10789	37673-37677	UBERON:0001970	denotes	bile
T10790	37673-37682	CHEBI:22868	denotes	bile salt
T10791	37733-37745	UBERON:0004711	denotes	jugular vein
T10792	37771-37775	CHEBI:80774	denotes	TUDC
T10793	37800-37804	UBERON:0001970	denotes	bile
T10794	37800-37809	CHEBI:22868	denotes	bile salt
T10795	37897-37901	CHEBI:80774	denotes	TUDC
T10796	37995-38007	CHEBI:16247	denotes	phospholipid
T10797	38080-38084	NCBITaxon:10088	denotes	mice
T10798	38099-38103	NCBITaxon:10088	denotes	mice
T10799	38128-38140	CHEBI:16247	denotes	phospholipid
T10800	38234-38241	UBERON:0002294	denotes	biliary
T10801	38242-38248	CHEBI:15889	denotes	sterol
T10802	38272-38278	CHEBI:15889	denotes	sterol
T10803	38367-38377	CHEBI:27693	denotes	sitosterol
T10804	38381-38392	CHEBI:16113	denotes	cholesterol
T10805	38437-38441	NCBITaxon:10088	denotes	mice
T10806	38464-38470	CHEBI:15889	denotes	sterol
T10807	38543-38547	NCBITaxon:10088	denotes	mice
T10808	38569-38573	NCBITaxon:10088	denotes	mice
T10809	38575-38581	CHEBI:15889	denotes	sterol
T10810	38673-38678	PR:000003573	denotes	Abcg8
T10811	38679-38689	PR:000003573	denotes	sterolin-2
T10812	38718-38725	UBERON:0002294	denotes	biliary
T10813	38727-38738	CHEBI:16113	denotes	cholesterol
T10814	38767-38771	NCBITaxon:10088	denotes	mice
T10815	38773-38779	CHEBI:15889	denotes	sterol
T10816	38887-38891	CHEBI:80774	denotes	TUDC
T10817	38903-38908	PR:000003573	denotes	Abcg8
T10818	38912-38916	NCBITaxon:10088	denotes	mice
T10819	38923-38931	GO:0046903	denotes	secreted
T10820	38937-38944	CHEBI:15889	denotes	sterols
T10821	38973-38980	UBERON:0002294	denotes	biliary
T10822	38981-38987	CHEBI:15889	denotes	sterol
T10823	39007-39015	GO:0046903	denotes	secreted
T10824	39027-39031	UBERON:0001970	denotes	bile
T10825	39051-39055	NCBITaxon:10088	denotes	mice
T10826	39086-39090	CHEBI:80774	denotes	TUDC
T10827	39111-39115	NCBITaxon:10088	denotes	Mice
T10828	39156-39160	UBERON:0001970	denotes	bile
T10829	39156-39165	CHEBI:3098	denotes	bile acid
T10830	39226-39230	CHEBI:80774	denotes	TUDC
T10831	39280-39284	NCBITaxon:10088	denotes	mice
T10832	39330-39337	GO:0046903	denotes	secrete
T10833	39338-39349	CHEBI:16113	denotes	cholesterol
T10834	39387-39394	GO:0046903	denotes	secrete
T10835	39395-39405	CHEBI:27693	denotes	sitosterol
T10836	39410-39421	CHEBI:28623	denotes	campesterol
T10837	39444-39448	NCBITaxon:10088	denotes	mice
T10838	39479-39484	PR:000003573	denotes	Abcg8
T10839	39488-39492	NCBITaxon:10088	denotes	mice
T10841	39544-39551	CHEBI:15889	denotes	sterols
T10842	39591-39595	NCBITaxon:10088	denotes	mice
T11818	39689-39700	CHEBI:16113	denotes	cholesterol
T11819	39742-39753	CHEBI:16113	denotes	cholesterol
T11820	39754-39761	CHEBI:15889	denotes	sterols
T11821	39814-39819	UBERON:0002107	denotes	liver
T11822	39836-39843	GO:0046903	denotes	secrete
T11823	39844-39851	CHEBI:15889	denotes	sterols
T11824	39899-39906	SO:0000704	denotes	genetic
T11825	39927-39932	NCBITaxon:9606	denotes	human
T11826	40138-40143	SO:0000704	denotes	genes
T11827	40256-40261	NCBITaxon:10088	denotes	mouse
T11828	40342-40347	SO:0000704	denotes	genes
T11829	40349-40354	PR:000003573	denotes	Abcg8
T11830	40361-40366	NCBITaxon:10088	denotes	mouse
T11831	40407-40412	NCBITaxon:9606	denotes	human
T11832	40438-40444	UBERON:0001969	denotes	Plasma
T11833	40453-40460	UBERON:0000479	denotes	tissues
T11834	40477-40482	UBERON:0000955	denotes	brain
T11835	40512-40522	CHEBI:27693	denotes	sitosterol
T11836	40551-40555	NCBITaxon:10088	denotes	mice
T11837	40651-40655	NCBITaxon:10088	denotes	mice
T11838	40802-40808	NCBITaxon:9606	denotes	humans
T11839	40826-40832	NCBITaxon:9606	denotes	humans
T11840	40850-40857	CHEBI:11814	denotes	HMG-CoA
T11841	40872-40878	PR:000006148	denotes	CYP7a1
T11842	40982-40988	UBERON:0002107	denotes	livers
T11843	41003-41010	NCBITaxon:33208	denotes	animals
T11844	41102-41106	UBERON:0001970	denotes	bile
T11845	41102-41111	CHEBI:3098	denotes	bile acid
T11846	41102-41121	GO:0006699	denotes	bile acid synthesis
T11847	41123-41129	PR:000006148	denotes	CYP7a1
T11848	41253-41263	CHEBI:27693	denotes	sitosterol
T11849	41316-41323	UBERON:0002294	denotes	Biliary
T11850	41324-41331	CHEBI:15889	denotes	sterols
T11851	41335-41340	PR:000003573	denotes	Abcg8
T11852	41351-41355	NCBITaxon:10088	denotes	mice
T11853	41406-41410	NCBITaxon:10088	denotes	mice
T11854	41427-41434	UBERON:0002294	denotes	biliary
T11855	41435-41441	CHEBI:15889	denotes	sterol
T11856	41461-41466	PR:000003573	denotes	Abcg8
T11857	41470-41474	NCBITaxon:10088	denotes	mice
T11858	41510-41516	CHEBI:15889	denotes	sterol
T11859	41532-41536	UBERON:0001970	denotes	bile
T11860	41567-41571	UBERON:0001970	denotes	bile
T11861	41567-41577	CHEBI:3098	denotes	bile acids
T11862	41609-41614	PR:000003573	denotes	Abcg8
T11863	41615-41625	PR:000003573	denotes	sterolin-2
T11864	41653-41660	GO:0046903	denotes	secrete
T11865	41661-41672	CHEBI:16113	denotes	cholesterol
T11866	41678-41682	UBERON:0001970	denotes	bile
T11867	41706-41716	CHEBI:27693	denotes	sitosterol
T11868	41776-41782	UBERON:0001969	denotes	plasma
T11869	41783-41793	CHEBI:27693	denotes	sitosterol
T11870	41817-41821	NCBITaxon:10088	denotes	mice
T11871	41864-41871	UBERON:0002294	denotes	biliary
T11872	41872-41882	CHEBI:27693	denotes	sitosterol
T11873	41980-41990	CHEBI:27693	denotes	sitosterol
T11874	42009-42013	UBERON:0001970	denotes	bile
T11875	42028-42033	NCBITaxon:3193	denotes	plant
T11876	42034-42041	CHEBI:15889	denotes	sterols
T11877	42049-42057	GO:0046903	denotes	secreted
T11878	42073-42078	PR:000003573	denotes	Abcg8
T11879	42079-42089	PR:000003573	denotes	sterolin-2
T11880	42106-42111	PR:000003573	denotes	Abcg8
T11881	42115-42119	NCBITaxon:10088	denotes	mice
T11882	42123-42130	GO:0046903	denotes	secrete
T11883	42131-42135	UBERON:0001970	denotes	bile
T11884	42131-42140	CHEBI:22868	denotes	bile salt
T11885	42145-42157	CHEBI:16247	denotes	phospholipid
T11886	42163-42167	UBERON:0001970	denotes	bile
T11887	42193-42197	NCBITaxon:10088	denotes	mice
T11888	42267-42272	PR:000003573	denotes	Abcg8
T11889	42273-42283	PR:000003573	denotes	sterolin-2
T11890	42301-42314	UBERON:0002423	denotes	hepatobiliary
T11891	42315-42326	CHEBI:16113	denotes	cholesterol
T11892	42340-42344	NCBITaxon:10088	denotes	mice
T11893	42346-42351	PR:000003573	denotes	Abcg8
T11894	42355-42359	NCBITaxon:10088	denotes	mice
T11895	42412-42418	NCBITaxon:9989	denotes	rodent
T11896	42419-42423	CHEBI:33290	denotes	chow
T11897	42506-42511	NCBITaxon:10088	denotes	mouse
T11898	42563-42569	UBERON:0001969	denotes	Plasma
T11899	42574-42581	UBERON:0002107	denotes	hepatic
T11900	42582-42587	NCBITaxon:3193	denotes	plant
T11901	42588-42594	CHEBI:15889	denotes	sterol
T11902	42605-42610	PR:000003573	denotes	Abcg8
T11903	42618-42623	PR:000003572	denotes	Abcg5
T11904	42624-42629	PR:000003573	denotes	Abcg8
T11905	42688-42699	CHEBI:16113	denotes	cholesterol
T11906	42723-42729	UBERON:0001969	denotes	plasma
T11907	42730-42742	CHEBI:17855	denotes	triglyceride
T11908	42757-42762	PR:000003573	denotes	Abcg8
T11909	42766-42770	NCBITaxon:10088	denotes	mice
T11910	42807-42814	NCBITaxon:33208	denotes	animals
T11911	42834-42846	CHEBI:17855	denotes	triglyceride
T11912	42966-42969	CHEBI:8984	denotes	SDS
T11913	43078-43085	UBERON:0002107	denotes	Hepatic
T11914	43108-43119	CHEBI:16113	denotes	cholesterol
T11915	43144-43149	PR:000003573	denotes	Abcg8
T11916	43153-43157	NCBITaxon:10088	denotes	mice
T11917	43180-43184	NCBITaxon:10088	denotes	mice
T11918	43205-43212	CHEBI:11814	denotes	HMG-CoA
T11919	43306-43312	PR:000028988	denotes	Srebps
T11920	43385-43390	NCBITaxon:3193	denotes	plant
T11921	43391-43398	CHEBI:15889	denotes	sterols
T11922	43425-43430	PR:000028988	denotes	SREBP
T11923	43443-43453	CHEBI:27693	denotes	Sitosterol
T11924	43489-43495	PR:000006148	denotes	Cyp7a1
T11925	43622-43626	NCBITaxon:10088	denotes	mice
T11926	43647-43657	CHEBI:27693	denotes	sitosterol
T11927	43853-43857	NCBITaxon:10088	denotes	mice
T11928	43876-43880	NCBITaxon:10088	denotes	mice
T11929	43902-43913	CHEBI:16113	denotes	cholesterol
T11930	43919-43929	CHEBI:27693	denotes	sitosterol
T11931	43983-43990	CHEBI:15889	denotes	sterols
T11932	44037-44042	NCBITaxon:9606	denotes	human
T11933	44090-44101	NCBITaxon:1	denotes	individuals
T11934	44122-44137	UBERON:0001621	denotes	coronary artery
T11935	44147-44152	PR:000003573	denotes	Abcg8
T11936	44153-44163	PR:000003573	denotes	sterolin-2
T11937	44174-44178	NCBITaxon:10088	denotes	mice
T11938	44232-44239	UBERON:0002294	denotes	biliary
T11939	44240-44246	CHEBI:15889	denotes	sterol
T11940	44262-44266	UBERON:0001970	denotes	bile
T11941	44270-44276	CHEBI:15889	denotes	sterol
T11942	44317-44321	UBERON:0001970	denotes	bile
T11943	44317-44326	CHEBI:22868	denotes	bile salt
T11944	44327-44336	GO:0007588	denotes	excretion
T11945	44338-44343	PR:000003573	denotes	Abcg8
T11946	44344-44354	PR:000003573	denotes	sterolin-2
T11947	44365-44369	NCBITaxon:10088	denotes	mice
T11948	44415-44419	NCBITaxon:10088	denotes	mice
T11949	44427-44432	PR:000003573	denotes	Abcg8
T11950	44433-44443	PR:000003573	denotes	sterolin-2
T11951	44461-44472	CHEBI:16113	denotes	cholesterol
T11952	44505-44510	NCBITaxon:3193	denotes	plant
T11953	44511-44517	CHEBI:15889	denotes	sterol
T11954	44566-44573	UBERON:0002294	denotes	biliary
T11955	44574-44580	CHEBI:15889	denotes	sterol
T11956	44628-44632	NCBITaxon:10088	denotes	mice
T11957	44677-44683	UBERON:0000479	denotes	tissue
T11958	44688-44694	UBERON:0001969	denotes	plasma
T11959	44704-44714	CHEBI:27693	denotes	sitosterol
T11960	44747-44751	NCBITaxon:10088	denotes	mice
T11961	44790-44800	CHEBI:27693	denotes	sitosterol
T11962	44834-44838	UBERON:0001970	denotes	bile
T11963	44920-44927	CHEBI:15889	denotes	sterols
T11964	44953-44959	NCBITaxon:9606	denotes	humans
T11965	44985-44992	SO:0000704	denotes	genetic
T11966	45011-45016	PR:000003572	denotes	ABCG5
T11967	45020-45025	PR:000003573	denotes	ABCG8
T11968	45060-45064	NCBITaxon:10088	denotes	mice
T11969	45082-45087	PR:000003573	denotes	Abcg8
T11970	45088-45098	PR:000003573	denotes	sterolin-2
T11971	45120-45126	CHEBI:15889	denotes	sterol
T11972	45211-45217	NCBITaxon:9989	denotes	rodent
T11973	45218-45222	CHEBI:33290	denotes	chow
T11974	45242-45249	NCBITaxon:33208	denotes	animals
T11975	45284-45290	UBERON:0001969	denotes	plasma
T11976	45294-45300	UBERON:0000479	denotes	tissue
T11977	45301-45311	CHEBI:27693	denotes	sitosterol
T11978	45423-45428	PR:000003573	denotes	Abcg8
T11979	45432-45436	NCBITaxon:10088	denotes	mice
T11980	45461-45468	UBERON:0002294	denotes	biliary
T11981	45469-45479	CHEBI:27693	denotes	sitosterol
T11982	45506-45510	NCBITaxon:10088	denotes	mice
T11983	45536-45543	UBERON:0002294	denotes	biliary
T11984	45544-45550	CHEBI:15889	denotes	sterol
T11985	45594-45598	NCBITaxon:10088	denotes	mice
T11986	45623-45627	NCBITaxon:10088	denotes	mice
T11987	45799-45806	GO:0007631	denotes	feeding
T11988	45937-45943	UBERON:0000479	denotes	tissue
T11989	45948-45954	UBERON:0001969	denotes	plasma
T11990	45955-45960	NCBITaxon:3193	denotes	plant
T11991	45961-45967	CHEBI:15889	denotes	sterol
T11992	45991-45998	NCBITaxon:33208	denotes	animals
T11993	46049-46056	NCBITaxon:33208	denotes	animals
T11994	46067-46072	NCBITaxon:3193	denotes	plant
T11995	46073-46079	CHEBI:15889	denotes	sterol
T11996	46103-46109	UBERON:0001969	denotes	plasma
T11997	46110-46116	CHEBI:15889	denotes	sterol
T11998	46164-46169	PR:000003572	denotes	Abcg5
T11999	46170-46175	PR:000003573	denotes	Abcg8
T12000	46181-46191	GO:0010467	denotes	expression
T12001	46218-46223	NCBITaxon:10088	denotes	mouse
T12002	46248-46255	UBERON:0002294	denotes	biliary
T12003	46256-46267	CHEBI:16113	denotes	cholesterol
T12004	46317-46326	CHEBI:4629	denotes	diosgenin
T12005	46327-46330	GO:0007631	denotes	fed
T12006	46331-46335	NCBITaxon:10088	denotes	mice
T12007	46364-46371	UBERON:0002294	denotes	biliary
T12008	46372-46383	CHEBI:16113	denotes	cholesterol
T12009	46407-46412	PR:000003572	denotes	Abcg5
T12010	46417-46422	PR:000003573	denotes	Abcg8
T12011	46491-46496	PR:000003572	denotes	Abcg5
T12012	46521-46530	CHEBI:4629	denotes	diosgenin
T12013	46541-46546	PR:000003573	denotes	Abcg8
T12014	46606-46613	UBERON:0002294	denotes	biliary
T12015	46614-46625	CHEBI:16113	denotes	cholesterol
T12016	46694-46701	UBERON:0002294	denotes	biliary
T12017	46702-46713	CHEBI:16113	denotes	cholesterol
T12018	46756-46761	PR:000003573	denotes	Abcg8
T12019	46772-46776	NCBITaxon:10088	denotes	mice
T12020	46798-46808	PR:000003573	denotes	sterolin-2
T12021	46850-46857	SO:0000704	denotes	genetic
T12022	46868-46874	UBERON:0001969	denotes	plasma
T12023	46875-46880	NCBITaxon:3193	denotes	plant
T12024	46881-46887	CHEBI:15889	denotes	sterol
T12025	46961-46972	GO:0065007	denotes	controlling
T12026	46973-46979	UBERON:0001969	denotes	plasma
T12027	46980-46985	NCBITaxon:3193	denotes	plant
T12028	46986-46992	CHEBI:15889	denotes	sterol
T12029	47040-47046	NCBITaxon:39107	denotes	murine
T12030	47135-47145	GO:0065007	denotes	regulating
T12031	47146-47152	UBERON:0001969	denotes	plasma
T12032	47153-47158	NCBITaxon:3193	denotes	plant
T12033	47159-47165	CHEBI:15889	denotes	sterol
T12034	47246-47252	CHEBI:15889	denotes	sterol
T12035	47246-47264	GO:0015918	denotes	sterol trafficking
T12036	47359-47364	PR:000003572	denotes	Abcg5
T12037	47365-47375	PR:000003572	denotes	sterolin-1
T12038	47380-47385	PR:000003573	denotes	Abcg8
T12039	47386-47396	PR:000003573	denotes	sterolin-2
T12040	47453-47458	PR:000003573	denotes	Abcg8
T12041	47459-47469	PR:000003573	denotes	sterolin-2
T12042	47553-47558	PR:000003573	denotes	Abcg8
T12043	47559-47569	PR:000003573	denotes	sterolin-2
T12044	47570-47580	GO:0010467	denotes	expression
T12045	47605-47611	GO:0045177	denotes	apical
T12046	47627-47632	PR:000003572	denotes	Abcg5
T12047	47633-47643	PR:000003572	denotes	sterolin-1
T12048	47651-47657	GO:0045177	denotes	apical
T12049	47658-47665	GO:0009986	denotes	surface
T12050	47725-47735	GO:0010467	denotes	expression
T12051	47822-47827	PR:000003572	denotes	Abcg5
T12052	47828-47838	PR:000003572	denotes	sterolin-1
T12053	47839-47849	GO:0042571	denotes	antibodies
T12054	47915-47920	PR:000003572	denotes	Abcg5
T12055	47921-47931	PR:000003572	denotes	sterolin-1
T12056	47935-47940	PR:000003573	denotes	Abcg8
T12057	47944-47948	NCBITaxon:10088	denotes	mice
T12058	47971-47986	MOP:0002162	denotes	N-glycosylation
T12066	48169-48177	GO:0045177	denotes	apically
T12067	48178-48187	GO:0010467	denotes	expressed
T12068	48195-48200	PR:000003573	denotes	Abcg8
T12069	48211-48215	NCBITaxon:10088	denotes	mice
T12070	48239-48244	PR:000003572	denotes	Abcg5
T12071	48245-48255	PR:000003572	denotes	sterolin-1
T12072	48256-48264	GO:0042571	denotes	antibody
T12073	48301-48306	PR:000003572	denotes	Abcg5
T12074	48307-48312	PR:000003573	denotes	Abcg8
T12075	48329-48335	NCBITaxon:9608	denotes	canine
T12076	48336-48358	UBERON:0002029	denotes	gallbladder epithelial
T12077	48336-48364	CL:1000415	denotes	gallbladder epithelial cells
T12078	48396-48411	GO:0005622	denotes	intracellularly
T12079	48459-48464	UBERON:0002107	denotes	liver
T12080	48459-48481	PR:000011395	denotes	liver X receptor alpha
T12081	48459-48475	_FRAGMENT	denotes	liver X receptor
T12082	48489-48496	CHEBI:86029	denotes	agonist
T12083	48483-48487	PR:000011395	denotes	LXRα
T12084	48498-48503	PR:000003572	denotes	Abcg5
T12085	48504-48514	PR:000003572	denotes	sterolin-1
T12086	48519-48524	PR:000003573	denotes	Abcg8
T12087	48525-48535	PR:000003573	denotes	sterolin-2
T12088	48551-48560	GO:0010467	denotes	expressed
T12089	48568-48574	GO:0045177	denotes	apical
T12090	48575-48582	GO:0009986	denotes	surface
T12091	48615-48620	PR:000003572	denotes	Abcg5
T12092	48621-48631	PR:000003572	denotes	sterolin-1
T12093	48632-48640	GO:0042571	denotes	antibody
T12094	48811-48817	GO:0045177	denotes	apical
T12095	48829-48832	NCBITaxon:10114	denotes	rat
T12096	48833-48837	PR:000015052	denotes	SPNT
T12097	48851-48867	UBERON:0004819	denotes	renal epithelial
T12098	48851-48873	CL:0002518	denotes	renal epithelial cells
T12099	48893-48908	MOP:0002162	denotes	N-glycosylation
T12100	48984-48989	PR:000003572	denotes	Abcg5
T12101	48990-49000	PR:000003572	denotes	sterolin-1
T12102	49001-49011	GO:0010467	denotes	expression
T12103	49033-49038	PR:000003573	denotes	Abcg8
T12104	49039-49049	PR:000003573	denotes	sterolin-2
T12105	49060-49064	NCBITaxon:10088	denotes	mice
T12106	49258-49263	PR:000003572	denotes	Abcg5
T12107	49264-49274	PR:000003572	denotes	sterolin-1
T12108	49282-49287	PR:000003573	denotes	Abcg8
T12109	49291-49295	NCBITaxon:10088	denotes	mice
T12110	49346-49351	PR:000003572	denotes	Abcg5
T12111	49352-49362	PR:000003572	denotes	sterolin-1
T12112	49373-49378	NCBITaxon:10088	denotes	mouse
T12113	49409-49416	GO:0046903	denotes	secrete
T12114	49417-49424	UBERON:0002294	denotes	biliary
T12115	49425-49436	CHEBI:16113	denotes	cholesterol
T12116	49473-49477	NCBITaxon:10088	denotes	mice
T12117	49488-49491	GO:0007631	denotes	fed
T12118	49494-49505	CHEBI:86029	denotes	LXR agonist
T12119	49518-49523	PR:000003573	denotes	Abcg8
T12120	49529-49539	GO:0010467	denotes	expression
T12121	49554-49561	GO:0046903	denotes	secrete
T12122	49567-49574	UBERON:0002294	denotes	biliary
T12123	49575-49586	CHEBI:16113	denotes	cholesterol
T12124	49602-49606	NCBITaxon:10088	denotes	mice
T12125	49637-49642	PR:000003572	denotes	Abcg5
T12126	49643-49653	PR:000003572	denotes	sterolin-1
T12127	49658-49663	PR:000003573	denotes	Abcg8
T12128	49664-49674	PR:000003573	denotes	sterolin-2
T12129	49722-49729	UBERON:0002107	denotes	hepatic
T12130	49730-49737	CHEBI:15889	denotes	sterols
T12131	49743-49747	UBERON:0001970	denotes	bile
T12132	49797-49802	PR:000003572	denotes	Abcg5
T12133	49813-49818	NCBITaxon:10088	denotes	mouse
T12134	49985-49991	NCBITaxon:33208	denotes	animal
T12135	50012-50017	PR:000003572	denotes	Abcg5
T12136	50018-50028	PR:000003572	denotes	sterolin-1
T12137	50033-50038	PR:000003573	denotes	Abcg8
T12138	50039-50049	PR:000003573	denotes	sterolin-2
T12139	50145-50152	GO:0046903	denotes	secrete
T12140	50153-50160	UBERON:0002294	denotes	biliary
T12141	50161-50168	CHEBI:15889	denotes	sterols
T14634	50259-50264	SO:0000704	denotes	genes
T14635	50365-50370	PR:000003573	denotes	Abcg8
T14636	50371-50381	PR:000003573	denotes	sterolin-2
T14637	50403-50410	UBERON:0002294	denotes	biliary
T14638	50411-50422	CHEBI:16113	denotes	cholesterol
T14639	50441-50446	PR:000003572	denotes	Abcg5
T14640	50447-50457	PR:000003572	denotes	sterolin-1
T14641	50478-50486	GO:0045177	denotes	apically
T14642	50487-50496	GO:0010467	denotes	expressed
T14643	50520-50525	PR:000003573	denotes	Abcg8
T14644	50526-50536	PR:000003573	denotes	sterolin-2
T14645	50585-50590	PR:000003573	denotes	Abcg8
T14646	50591-50601	PR:000003573	denotes	sterolin-2
T14647	50621-50628	UBERON:0002294	denotes	biliary
T14648	50629-50640	CHEBI:16113	denotes	cholesterol
T14649	50661-50668	UBERON:0002294	denotes	biliary
T14650	50669-50679	CHEBI:27693	denotes	sitosterol
T14651	50772-50777	PR:000003573	denotes	Abcg8
T14652	50778-50788	PR:000003573	denotes	sterolin-2
T14653	50821-50831	CHEBI:27693	denotes	sitosterol
T14654	50837-50841	UBERON:0001970	denotes	bile
T12059	48019-48024	PR:000003572	denotes	Abcg5
T12060	48025-48035	PR:000003572	denotes	sterolin-1
T12061	48044-48049	PR:000003573	denotes	Abcg8
T12062	48053-48057	NCBITaxon:10088	denotes	mice
T12063	48077-48098	GO:0005783	denotes	endoplasmic reticulum
T12064	48149-48154	PR:000003572	denotes	Abcg5
T12065	48155-48165	PR:000003572	denotes	sterolin-1
R354	T783	T782	_lexicallyChainedTo	cholesterol from,Absorption of
R355	T784	T783	_lexicallyChainedTo	intestine,cholesterol from
R2313	T3630	T3629	_lexicallyChainedTo	DNA,amplification of
R4952	T7353	T7352	_lexicallyChainedTo	protein,production of
R5276	T7945	T7944	_lexicallyChainedTo	sterols,entry of
R5277	T7985	T7984	_lexicallyChainedTo	E,apolipoproteins
R5278	T7986	T7984	_lexicallyChainedTo	AI,apolipoproteins
R5279	T7987	T7984	_lexicallyChainedTo	AII,apolipoproteins
R7545	T12082	T12081	_lexicallyChainedTo	agonist,liver X receptor

2_test

Id	Subject	Object	Predicate	Lexical cue
15040800-659760-12037272	2570-2571	659760	denotes	1
15040800-6486101-12037272	2570-2571	6486101	denotes	1
15040800-8077852-12037272	2570-2571	8077852	denotes	1
15040800-4360855-12037273	3309-3310	4360855	denotes	5
15040800-1246333-12037274	3455-3456	1246333	denotes	6
15040800-1431587-12037275	3457-3458	1431587	denotes	7
15040800-2600539-12037276	3956-3957	2600539	denotes	8
15040800-1911714-12037277	3958-3959	1911714	denotes	9
15040800-1431587-12037278	4171-4172	1431587	denotes	7
15040800-1911714-12037279	4173-4174	1911714	denotes	9
15040800-9610773-12037280	4360-4362	9610773	denotes	10
15040800-9727073-12037281	4363-4365	9727073	denotes	11
15040800-11378826-12037282	4506-4508	11378826	denotes	12
15040800-11378825-12037283	4509-4511	11378825	denotes	13
15040800-11099417-12037284	4772-4774	11099417	denotes	14
15040800-11452359-12037284	4772-4774	11452359	denotes	14
15040800-11138003-12037284	4772-4774	11138003	denotes	14
15040800-11099417-12037285	4910-4912	11099417	denotes	14
15040800-12208868-12037286	5670-5672	12208868	denotes	17
15040800-12208867-12037287	5933-5935	12208867	denotes	18
15040800-14504269-12037288	5936-5938	14504269	denotes	19
15040800-12444248-12037289	6379-6381	12444248	denotes	20
15040800-11907139-12037290	7613-7615	11907139	denotes	21
15040800-1195397-12037291	11737-11739	1195397	denotes	22
15040800-10600799-12037292	11919-11921	10600799	denotes	23
15040800-12105210-12037293	12883-12885	12105210	denotes	24
15040800-12097330-12037293	12883-12885	12097330	denotes	24
15040800-11717426-12037293	12883-12885	11717426	denotes	24
15040800-10359567-12037294	15715-15717	10359567	denotes	27
15040800-3357417-12037295	17026-17028	3357417	denotes	28
15040800-12444248-12037296	17273-17275	12444248	denotes	20
15040800-12783625-12037297	17276-17278	12783625	denotes	29
15040800-12763362-12037298	17279-17281	12763362	denotes	30
15040800-6699096-12037299	18306-18308	6699096	denotes	31
15040800-8869755-12037300	22725-22727	8869755	denotes	32
15040800-14907713-12037301	24193-24195	14907713	denotes	34
15040800-7706454-12037302	25878-25880	7706454	denotes	35
15040800-4811219-12037303	25915-25917	4811219	denotes	36
15040800-6854150-12037304	30209-30211	6854150	denotes	37
15040800-7119575-12037305	30212-30214	7119575	denotes	38
15040800-1431587-12037306	32223-32224	1431587	denotes	7
15040800-3143743-12037307	33010-33012	3143743	denotes	39
15040800-12208867-12037308	33886-33888	12208867	denotes	18
15040800-12763362-12037309	33889-33891	12763362	denotes	30
15040800-12763362-12037310	36131-36133	12763362	denotes	30
15040800-5441548-12037311	37037-37039	5441548	denotes	40
15040800-6768564-12037312	37040-37042	6768564	denotes	41
15040800-4360855-12037313	40429-40430	4360855	denotes	5
15040800-1431587-12037314	40431-40432	1431587	denotes	7
15040800-1431587-12037315	40909-40910	1431587	denotes	7
15040800-3143743-12037316	40911-40913	3143743	denotes	39
15040800-3143743-12037317	41311-41313	3143743	denotes	39
15040800-1431587-12037318	42556-42557	1431587	denotes	7
15040800-12444248-12037319	42558-42560	12444248	denotes	20
15040800-3143743-12037320	43579-43581	3143743	denotes	39
15040800-12763362-12037321	46285-46287	12763362	denotes	30
15040800-12446833-12037322	47060-47062	12446833	denotes	43
15040800-12208867-12037323	47749-47751	12208867	denotes	18
15040800-14504269-12037324	47752-47754	14504269	denotes	19
15040800-12878214-12037325	48439-48441	12878214	denotes	44
15040800-12636174-12037326	48910-48912	12636174	denotes	45
15040800-14699507-12037327	49608-49610	14699507	denotes	46
15040800-14504269-12037328	49749-49751	14504269	denotes	19

PMC:394351 JSONTXT

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craft-sa-dev

craft-ca-core-ex-dev

craft-ca-core-dev

2_test

PMC:394351 JSON TXT