PMC:3907684 / 8162-9182 JSONTXT

{"project":"2_test","denotations":[{"id":"24490137-10096545-30594229","span":{"begin":434,"end":436},"obj":"10096545"},{"id":"24490137-10449618-30594230","span":{"begin":604,"end":605},"obj":"10449618"},{"id":"24490137-6259064-30594231","span":{"begin":606,"end":607},"obj":"6259064"},{"id":"24490137-16688717-30594232","span":{"begin":608,"end":610},"obj":"16688717"}],"text":"2.1 Sample preparation, DNA and RNA extraction\nEarly passages of NPC cell lines, HK1, C666, and NP460, and the X666 xenograft were utilized for this study. DNAs were extracted from cell lines and the xenograft using the QIAGEN DNA extraction kit according to manufacturer instructions. DNA genotyping of cell lines authenticated their origins. Real-time quantitative EBV DNA PCR was performed on cell lines, as previously described [29]. EBV DNA was only detected in C666 and no EBV DNA was detected in HK1 and NP460 cell lines (data not shown). All the cell lines were re-grown in standard conditions [6,8,10]. Cells were collected from three subsequent culture passages upon reaching ∼80% confluence, as described. Total RNA was extracted with TRIZOL reagent (Invitrogen, NY, USA) according to standard procedures. Integrity of RNA was checked by Agilent 2100 bioanalyzer using the RNA 6000 Nano total RNA assay, with RIN value \u003e8.9. RNA from the earliest passage of each cell line was selected for Solexa sequencing."}

{"project":"MyTest","denotations":[{"id":"24490137-10096545-30594229","span":{"begin":434,"end":436},"obj":"10096545"},{"id":"24490137-10449618-30594230","span":{"begin":604,"end":605},"obj":"10449618"},{"id":"24490137-6259064-30594231","span":{"begin":606,"end":607},"obj":"6259064"},{"id":"24490137-16688717-30594232","span":{"begin":608,"end":610},"obj":"16688717"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"2.1 Sample preparation, DNA and RNA extraction\nEarly passages of NPC cell lines, HK1, C666, and NP460, and the X666 xenograft were utilized for this study. DNAs were extracted from cell lines and the xenograft using the QIAGEN DNA extraction kit according to manufacturer instructions. DNA genotyping of cell lines authenticated their origins. Real-time quantitative EBV DNA PCR was performed on cell lines, as previously described [29]. EBV DNA was only detected in C666 and no EBV DNA was detected in HK1 and NP460 cell lines (data not shown). All the cell lines were re-grown in standard conditions [6,8,10]. Cells were collected from three subsequent culture passages upon reaching ∼80% confluence, as described. Total RNA was extracted with TRIZOL reagent (Invitrogen, NY, USA) according to standard procedures. Integrity of RNA was checked by Agilent 2100 bioanalyzer using the RNA 6000 Nano total RNA assay, with RIN value \u003e8.9. RNA from the earliest passage of each cell line was selected for Solexa sequencing."}