PMC:3886832 / 37786-39452
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3886832","sourcedb":"PMC","sourceid":"3886832","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3886832","text":"5.1. Antibody Microarrays\nProteins can be separated and characterized via a number of different techniques (reviewed in [30]). One of these techniques is the use of antibody microarrays, which are similar to DNA microarrays, except that antibodies are printed on the chip instead of DNA probes [52]. Antibody microarrays were first used by Jamesdaniel et al. [53] to study hair cell damage in the inner ears of rats treated with cisplatin. This study identified the dynamic changes of 19 proteins that were either up- or downregulated and 15 of those were novel proteins that belong to either cell death or survival pathways [53]. Differential expression of proteins in the organ of Corti, lateral wall and modiolus of chinchilla showed enrichment of cell death pathways in both the sensory epithelia and modiolus [54]. Immunolabeling experiments confirmed the antibody microarray results by identifying the expression of E2F3 in nuclei and WSTF and FAK-p-Tyr577 in the stereocilia in the organ of Corti. Detection of FAK-p-Tyr577 supports the idea that post-translational modification of proteins can be identified using antibody microarrays, but cannot be detected using gene arrays [54]. It is necessary to have a large amount of tissue to study proteomics, and this problem was circumvented by an approach called “Subtractive Strategy Using Mouse Mutants” that can detect large number of proteins with only a small amount of starting material. Using the Pou4f3 mutant mouse model, the association of Pou4f3 in the inner ear with other genes, such as Notch1-4, gata3, p27Kip1, Eya1, S100a1, Tbx3, Shh, Fgfr3, Bmp5, Jag2 and Fkh10, were able to be identified [50].","divisions":[{"label":"Title","span":{"begin":0,"end":25}}],"tracks":[{"project":"2_test","denotations":[{"id":"24416530-16540098-69475191","span":{"begin":121,"end":123},"obj":"16540098"},{"id":"24416530-16540098-69475191","span":{"begin":121,"end":123},"obj":"16540098"},{"id":"24416530-18578524-69475192","span":{"begin":360,"end":362},"obj":"18578524"},{"id":"24416530-18578524-69475192","span":{"begin":360,"end":362},"obj":"18578524"},{"id":"24416530-18578524-69475193","span":{"begin":626,"end":628},"obj":"18578524"},{"id":"24416530-18578524-69475193","span":{"begin":626,"end":628},"obj":"18578524"},{"id":"24416530-21871588-69475194","span":{"begin":815,"end":817},"obj":"21871588"},{"id":"24416530-21871588-69475194","span":{"begin":815,"end":817},"obj":"21871588"},{"id":"24416530-21871588-69475195","span":{"begin":1186,"end":1188},"obj":"21871588"},{"id":"24416530-21871588-69475195","span":{"begin":1186,"end":1188},"obj":"21871588"},{"id":"24416530-16600193-69475196","span":{"begin":1662,"end":1664},"obj":"16600193"},{"id":"24416530-16600193-69475196","span":{"begin":1662,"end":1664},"obj":"16600193"},{"id":"T60137","span":{"begin":121,"end":123},"obj":"16540098"},{"id":"T33493","span":{"begin":121,"end":123},"obj":"16540098"},{"id":"T36001","span":{"begin":360,"end":362},"obj":"18578524"},{"id":"T95580","span":{"begin":360,"end":362},"obj":"18578524"},{"id":"T43472","span":{"begin":626,"end":628},"obj":"18578524"},{"id":"T7460","span":{"begin":626,"end":628},"obj":"18578524"},{"id":"T58784","span":{"begin":815,"end":817},"obj":"21871588"},{"id":"T22695","span":{"begin":815,"end":817},"obj":"21871588"},{"id":"T50863","span":{"begin":1186,"end":1188},"obj":"21871588"},{"id":"T85781","span":{"begin":1186,"end":1188},"obj":"21871588"},{"id":"T85150","span":{"begin":1662,"end":1664},"obj":"16600193"},{"id":"T95432","span":{"begin":1662,"end":1664},"obj":"16600193"}],"attributes":[{"subj":"24416530-16540098-69475191","pred":"source","obj":"2_test"},{"subj":"24416530-16540098-69475191","pred":"source","obj":"2_test"},{"subj":"24416530-18578524-69475192","pred":"source","obj":"2_test"},{"subj":"24416530-18578524-69475192","pred":"source","obj":"2_test"},{"subj":"24416530-18578524-69475193","pred":"source","obj":"2_test"},{"subj":"24416530-18578524-69475193","pred":"source","obj":"2_test"},{"subj":"24416530-21871588-69475194","pred":"source","obj":"2_test"},{"subj":"24416530-21871588-69475194","pred":"source","obj":"2_test"},{"subj":"24416530-21871588-69475195","pred":"source","obj":"2_test"},{"subj":"24416530-21871588-69475195","pred":"source","obj":"2_test"},{"subj":"24416530-16600193-69475196","pred":"source","obj":"2_test"},{"subj":"24416530-16600193-69475196","pred":"source","obj":"2_test"},{"subj":"T60137","pred":"source","obj":"2_test"},{"subj":"T33493","pred":"source","obj":"2_test"},{"subj":"T36001","pred":"source","obj":"2_test"},{"subj":"T95580","pred":"source","obj":"2_test"},{"subj":"T43472","pred":"source","obj":"2_test"},{"subj":"T7460","pred":"source","obj":"2_test"},{"subj":"T58784","pred":"source","obj":"2_test"},{"subj":"T22695","pred":"source","obj":"2_test"},{"subj":"T50863","pred":"source","obj":"2_test"},{"subj":"T85781","pred":"source","obj":"2_test"},{"subj":"T85150","pred":"source","obj":"2_test"},{"subj":"T95432","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#a7ec93","default":true}]}]}}