PMC:3873243 / 50857-51587 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3873243","sourcedb":"PMC","sourceid":"3873243","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3873243","text":"Mitochondrial translation assay\nzMitochondrial translation assays were performed as described previously [58], [59]. Briefly, fibroblasts were cultured with cycloheximide to inhibit cytoplasmic translation and mtDNA-encoded proteins were labeled with a 2-hour pulse of 35S-methionine/35S-cysteine (EXPRE35S35S Protein Labeling Mix; Perkin Elmer Life Sciences) prior to washing and a chase with cold methionine for 0 to 24 hours. Mitochondria were then isolated and translation products were analyzed by SDS-PAGE and autoradiography. HEK293 cells were cultured and labeled in the same way, except that labeling was done for 1 hour, emetine was used instead of cycloheximide and Tran35S-Label (MP Biomedicals) was used for labeling.","divisions":[{"label":"Title","span":{"begin":0,"end":31}}],"tracks":[{"project":"2_test","denotations":[{"id":"24385928-19348897-86086215","span":{"begin":106,"end":108},"obj":"19348897"},{"id":"24385928-1334369-86086216","span":{"begin":112,"end":114},"obj":"1334369"},{"id":"T78392","span":{"begin":106,"end":108},"obj":"19348897"},{"id":"T33713","span":{"begin":112,"end":114},"obj":"1334369"}],"attributes":[{"subj":"24385928-19348897-86086215","pred":"source","obj":"2_test"},{"subj":"24385928-1334369-86086216","pred":"source","obj":"2_test"},{"subj":"T78392","pred":"source","obj":"2_test"},{"subj":"T33713","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#9394ec","default":true}]}]}}