PMC:3832756 / 16995-18596
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3832756","sourcedb":"PMC","sourceid":"3832756","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3832756","text":"Recently, we reported that SFN inhibited mTOR-S6K1 signaling and translation process in PC-3 prostate and SKBR-3 breast cancer cell lines [30]. Results presented in previous subsections of this work suggest that SFN inhibits mTOR signaling in other breast cancer cells. As mTOR is a master activator of mRNA translation, we investigated the impact of SFN on protein synthesis in MDA MB 231, MCF-7 and MDA MB 468 cells. We measured [3H]-leucine incorporation in control, DMSO-treated cells and cells exposed to 10, 20 or 40 μM SFN for 3 h. As demonstrated in Fig. 5, SFN inhibited [3H]-leucine incorporation in a dose-dependent manner. Protein synthesis in MDA MB 231 cells measured after 3-h exposure to 10, 20 or 40 μM SFN dropped to about 75, 67 and 44 % of the level seen in control cells, respectively. Similarly, in MCF-7 translation decreased to about 60, 40 and 20 % and in MDA MB 468 to 78, 40 and 20 % of control cells level upon treatment with 10, 20 or 40 μM SFN, respectively (Fig. 5).\nFig. 5 SFN inhibits protein synthesis in human breast cancer cells. MDA MB 231, MCF-7, MDA MB 468 were treated with various concentrations of SFN for 3 h in the presence of a protein precursor, [3H] –leucine. Cells were harvested, and radioactivity of TCA-precipitable material was estimated as described in “Materials and methods”. Results shown are mean ± SE of two independent experiments performed in duplicate (MDA MB 231 and MCF-7) or in triplicate (MDA MB 468), *significantly different (P \u003c 0.01) compared with DMSO-treated control by one-way ANOVA followed by Dunnett’s multiple comparison test","divisions":[{"label":"label","span":{"begin":998,"end":1004}}],"tracks":[{"project":"2_test","denotations":[{"id":"23389114-22640870-29182878","span":{"begin":139,"end":141},"obj":"22640870"}],"attributes":[{"subj":"23389114-22640870-29182878","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"23389114-22640870-29182878","span":{"begin":139,"end":141},"obj":"22640870"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"23389114-22640870-29182878","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#9397ec","default":true},{"id":"MyTest","color":"#b1ec93"}]}]}}