PMC:3832756 / 11431-12424 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3832756","sourcedb":"PMC","sourceid":"3832756","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3832756","text":"We examined the effects of SFN on viability of four breast cancer cell lines: MDA MB 231, MCF-7, MDA MB 468 and SKBR-3. These cell lines have been chosen as they represent examples of different abnormalities in PI3K-Akt-mTOR-S6K1 signaling pathway (Table 1). MTT assay revealed that SFN inhibited viability of all cell lines in a dose-dependent manner (Fig. 1). Moreover, anti-proliferative activity of SFN was similar in the case of all four cell lines, regardless of their PI3K-Akt-mTOR-S6K1 status, with IC50 ranging from 19 μM (for MCF-7) to 25 μM (for SKBR-3) after 24 h of treatment (Table 1).\nFig. 1 Sulforaphane decreases viability of phenotypically different cells in a dose-dependent manner. MDA MB 231, MCF-7, MDA MB 468 and SKBR-3 cells were treated with DMSO (0) or different concentrations of SFN (5, 10, 20, 30 or 40 μM) for 24 h. Their viability was assayed by MTT method as described in “Materials and methods”. Each point is mean (±SE) of three experiments done in triplicate","divisions":[{"label":"label","span":{"begin":600,"end":606}}],"tracks":[]}