PMC:3813744 / 18277-21271 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3813744","sourcedb":"PMC","sourceid":"3813744","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3813744","text":"Experimental Designs and Statistical Analysis\nExperiment 1 assessed the effects of the 5-HT2CR antagonist SB242084 on reversal learning, perseverance and learned non-reward using a between-subjects design. After completing the spatial discrimination drug-free, animals were pair-matched for trials to criterion and randomly assigned to a drug and test condition. Animals subsequently completed one of a full reversal test, perseverance test, or learned non-reward test.\nExperiment 2 assessed 5-HT2CR KO mice on discrimination learning, reversal learning, perseverance and learned non-reward using a within-subjects design. All animals completed an initial spatial discrimination followed by a full reversal test. This was followed by a learned non-reward test and a perseverance test. The order of the perseverance and the learned non-reward tests was counterbalanced across the two genotypes.\nIn both Experiments 1 and 2, animals were allowed a maximum of 10 days (i.e., 250 trials) to reach criterion within each test condition. Animals failing to reach criterion on a test were assigned a trial-score of 250 for that test and not tested further. The dependent variables collected from each test condition were probe-trials, trials, correct responses and incorrect responses to criterion. To analyse performances in the early and late phases of learning, trials to criterion were broken down into 10-trial bins. Incorrect responses made before achieving 50% correct responses in a 10-trial bin were coded as early errors. Incorrect responses made once the animal achieved ≤50% correct responses in a 10-trial bin were coded as late errors.\nIn Experiment 1, the data was analysed by 2 (drug) × 3 (test condition) between-subject ANOVAs. Significant interactions were followed-up by separate ANOVAs or LSD post-hoc comparisons. In Experiment 2, a number of predominantly KO animals failed to complete criterion across all tests within 250 trials. Genotype differences in percent achieving criterion within each test was initially investigated by analysing the distribution of failing or passing through chi-square distribution analysis. Trials, correct and incorrect responses to criterion for each test was then analysed through one-way between-subject ANOVAs with genotype as the independent variable. Behavioural analyses only included animals attempting a given test.\nIn Experiment 3, animals were dosed with vehicle or 0.5 mg/kg of SB242084 15 min before testing. Hence, the novel-arm was introduced 30 min after drug treatment, as in Experiment 1. The 2×15 min test phases (pre- and post-change) were recorded and analysed using JWatcher (version 1.0). The proportion of time spent in each arm and the proportion of arm entries made into each arm was scored before and after the 45° change in response arm location. An arm-entry was scored once an animal had moved far enough into the arm that its hind paws were beyond the location of the insert between the central platform and the arm.","divisions":[{"label":"Title","span":{"begin":0,"end":45}}],"tracks":[]}