PMC:3813744 / 10758-11758 JSONTXT

{"project":"NEUROSES","denotations":[{"id":"T174","span":{"begin":267,"end":271},"obj":"PATO_0000384"},{"id":"T175","span":{"begin":361,"end":365},"obj":"PATO_0000384"},{"id":"T176","span":{"begin":267,"end":271},"obj":"CHEBI_30780"},{"id":"T177","span":{"begin":361,"end":365},"obj":"CHEBI_30780"},{"id":"T178","span":{"begin":703,"end":711},"obj":"CHEBI_7507"},{"id":"T179","span":{"begin":934,"end":937},"obj":"PATO_0000308"},{"id":"T180","span":{"begin":939,"end":942},"obj":"CHEBI_84123"},{"id":"T181","span":{"begin":939,"end":942},"obj":"PATO_0000011"}],"text":"Breeding and genotyping\nThe 5-HT2CR KO and WT animals used in Experiment 2 were of a C57BL/6J background generated as previously described [16]. The original progeny of 5-HT2CR KO mice used here were a gift from L. Tecott and produced as described by [29]. Wild-type male mice were crossed with females heterozygous for the X-linked 5-HT2CR mutation generating male WT and KO offspring. Genotyping was achieved using PCR on tissue samples from ear punches. The wild-type allele was detected using primers of the 5-HT2CR gene sequences flanking the Neo insertion: m5h2c (5′-AGTTGATGTTCATCTCAGGTGGC-3′) and 3N2 (5′-GGGTCCTATAGATCGAGGTACC-3′). The mutant allele was detected using primers complimentary to neomycin resistance gene (Neo) sequences: NeoD (5′-CACCTTGCTCCTGCCGAGAAA-3′) and NeoH (5′-AGAAGGCGATAGAAGGCGATG-3′). Breeding animals had been backcrossed for more than 20 generations and the individuals used here were 10–24 weeks old (age-matched for genotype) at the beginning of the experiment."}

{"project":"2_test","denotations":[{"id":"24204954-22644128-87518370","span":{"begin":140,"end":142},"obj":"22644128"},{"id":"24204954-7700379-87518371","span":{"begin":252,"end":254},"obj":"7700379"},{"id":"T63954","span":{"begin":140,"end":142},"obj":"22644128"},{"id":"T63842","span":{"begin":252,"end":254},"obj":"7700379"}],"text":"Breeding and genotyping\nThe 5-HT2CR KO and WT animals used in Experiment 2 were of a C57BL/6J background generated as previously described [16]. The original progeny of 5-HT2CR KO mice used here were a gift from L. Tecott and produced as described by [29]. Wild-type male mice were crossed with females heterozygous for the X-linked 5-HT2CR mutation generating male WT and KO offspring. Genotyping was achieved using PCR on tissue samples from ear punches. The wild-type allele was detected using primers of the 5-HT2CR gene sequences flanking the Neo insertion: m5h2c (5′-AGTTGATGTTCATCTCAGGTGGC-3′) and 3N2 (5′-GGGTCCTATAGATCGAGGTACC-3′). The mutant allele was detected using primers complimentary to neomycin resistance gene (Neo) sequences: NeoD (5′-CACCTTGCTCCTGCCGAGAAA-3′) and NeoH (5′-AGAAGGCGATAGAAGGCGATG-3′). Breeding animals had been backcrossed for more than 20 generations and the individuals used here were 10–24 weeks old (age-matched for genotype) at the beginning of the experiment."}