PMC:3807239 / 26514-27318 JSONTXT

{"project":"2_test","denotations":[{"id":"24163754-18079988-112081902","span":{"begin":789,"end":790},"obj":"18079988"},{"id":"24163754-18079988-112081902","span":{"begin":789,"end":790},"obj":"18079988"},{"id":"24163754-19933824-112081903","span":{"begin":791,"end":793},"obj":"19933824"},{"id":"24163754-19933824-112081903","span":{"begin":791,"end":793},"obj":"19933824"},{"id":"24163754-9822382-112081904","span":{"begin":794,"end":796},"obj":"9822382"},{"id":"24163754-9822382-112081904","span":{"begin":794,"end":796},"obj":"9822382"},{"id":"24163754-10430607-112081904","span":{"begin":794,"end":796},"obj":"10430607"},{"id":"24163754-10430607-112081904","span":{"begin":794,"end":796},"obj":"10430607"},{"id":"24163754-16609010-112081904","span":{"begin":794,"end":796},"obj":"16609010"},{"id":"24163754-16609010-112081904","span":{"begin":794,"end":796},"obj":"16609010"},{"id":"24163754-18079988-69479706","span":{"begin":789,"end":790},"obj":"18079988"},{"id":"24163754-18079988-69479706","span":{"begin":789,"end":790},"obj":"18079988"},{"id":"24163754-19933824-69479707","span":{"begin":791,"end":793},"obj":"19933824"},{"id":"24163754-19933824-69479707","span":{"begin":791,"end":793},"obj":"19933824"},{"id":"24163754-9822382-69479708","span":{"begin":794,"end":796},"obj":"9822382"},{"id":"24163754-9822382-69479708","span":{"begin":794,"end":796},"obj":"9822382"},{"id":"24163754-10430607-69479709","span":{"begin":797,"end":799},"obj":"10430607"},{"id":"24163754-10430607-69479709","span":{"begin":797,"end":799},"obj":"10430607"},{"id":"24163754-16609010-69479710","span":{"begin":800,"end":802},"obj":"16609010"},{"id":"24163754-16609010-69479710","span":{"begin":800,"end":802},"obj":"16609010"}],"text":"6.1. HCT-116 Cell Lines\nTwo cell lines of HCT-116 (wt-p53 and null-p53) were treated with three drugs: 5-fluorouracil (5-FU), oxaliplatin (OX) and irinotecan (CPT-11). The total RNAs were isolated from the six treated cell lines and two non-treated cell lines (serving as controls) and tested with miRCURY LNA MicroRNA Array v7.5.0 (Exiqon Inc.). For HCT-116 (null-p53), the control sample was tested three times, and the CPT-11 treated sample was tested twice. For HCT-116 (wt-p53), both the control sample and the 5-FU treated sample were tested twice. A total of 359 miRNAs were profiled for each of the 13 cell lines. A total of 37 miRNAs were randomly selected and further tested using TaqMan-based qRT-PCR on an ABI 7500HT instrument (Applied Biosystems Inc., Foster City, CA, USA) [9,47,55,56,57]."}