PMC:3728556 / 6085-7331 JSONTXT

{"project":"TEST0","denotations":[{"id":"23914152-112-120-639527","span":{"begin":120,"end":124},"obj":"[\"18551525\"]"}],"text":"Animals\nWfs1-deficient mice were generated by invalidating the 8th exon of the Wfs1 gene (for details, see Luuk et al., 2008). Experiments were performed in 3–4 months old male and female F2 hybrids [(129S6/SvEvTac × C57BL/6) × (129S6/SvEvTac × C57BL/6)]. Two separate batches of naïve animals were used for the FST and TST. For the gene expression measurements, naïve animals, taken directly from their home-cages, were used. For the monoamine measurement studies, both naïve mice and mice exposed to motility boxes were chosen. Breeding and genotyping were conducted in the Department of Physiology, University of Tartu. The animals were kept in groups of eight per cage at 22 ± 1°C in a room illuminated artificially from 7 am to 7 pm. Tap water and food pellets were freely available. The permission (No. 88, 25th of August, 2011) for the present study was given by the Estonian National Board of Animal Experiments in accordance with the European Communities Directive of 24 November 1986 (86/609/EEC). Behavioral experiments were carried out between 10:00 and 17:00. Wfs1-deficient homozygous mice were always used in parallel with their wild-type and heterozygous littermates and the animals were randomly divided into experimental groups."}

{"project":"0_colil","denotations":[{"id":"23914152-18551525-639527","span":{"begin":120,"end":124},"obj":"18551525"}],"text":"Animals\nWfs1-deficient mice were generated by invalidating the 8th exon of the Wfs1 gene (for details, see Luuk et al., 2008). Experiments were performed in 3–4 months old male and female F2 hybrids [(129S6/SvEvTac × C57BL/6) × (129S6/SvEvTac × C57BL/6)]. Two separate batches of naïve animals were used for the FST and TST. For the gene expression measurements, naïve animals, taken directly from their home-cages, were used. For the monoamine measurement studies, both naïve mice and mice exposed to motility boxes were chosen. Breeding and genotyping were conducted in the Department of Physiology, University of Tartu. The animals were kept in groups of eight per cage at 22 ± 1°C in a room illuminated artificially from 7 am to 7 pm. Tap water and food pellets were freely available. The permission (No. 88, 25th of August, 2011) for the present study was given by the Estonian National Board of Animal Experiments in accordance with the European Communities Directive of 24 November 1986 (86/609/EEC). Behavioral experiments were carried out between 10:00 and 17:00. Wfs1-deficient homozygous mice were always used in parallel with their wild-type and heterozygous littermates and the animals were randomly divided into experimental groups."}

{"project":"2_test","denotations":[{"id":"23914152-18551525-38522475","span":{"begin":120,"end":124},"obj":"18551525"}],"text":"Animals\nWfs1-deficient mice were generated by invalidating the 8th exon of the Wfs1 gene (for details, see Luuk et al., 2008). Experiments were performed in 3–4 months old male and female F2 hybrids [(129S6/SvEvTac × C57BL/6) × (129S6/SvEvTac × C57BL/6)]. Two separate batches of naïve animals were used for the FST and TST. For the gene expression measurements, naïve animals, taken directly from their home-cages, were used. For the monoamine measurement studies, both naïve mice and mice exposed to motility boxes were chosen. Breeding and genotyping were conducted in the Department of Physiology, University of Tartu. The animals were kept in groups of eight per cage at 22 ± 1°C in a room illuminated artificially from 7 am to 7 pm. Tap water and food pellets were freely available. The permission (No. 88, 25th of August, 2011) for the present study was given by the Estonian National Board of Animal Experiments in accordance with the European Communities Directive of 24 November 1986 (86/609/EEC). Behavioral experiments were carried out between 10:00 and 17:00. Wfs1-deficient homozygous mice were always used in parallel with their wild-type and heterozygous littermates and the animals were randomly divided into experimental groups."}

{"project":"NEUROSES","denotations":[{"id":"T207","span":{"begin":843,"end":850},"obj":"PATO_0000467"},{"id":"T208","span":{"begin":1213,"end":1220},"obj":"PATO_0001786"},{"id":"T195","span":{"begin":168,"end":171},"obj":"PATO_0000308"},{"id":"T196","span":{"begin":172,"end":176},"obj":"PATO_0000384"},{"id":"T197","span":{"begin":172,"end":176},"obj":"CHEBI_30780"},{"id":"T198","span":{"begin":181,"end":187},"obj":"PATO_0000383"},{"id":"T199","span":{"begin":404,"end":408},"obj":"CHEBI_75830"},{"id":"T200","span":{"begin":435,"end":444},"obj":"CHEBI_63534"},{"id":"T201","span":{"begin":435,"end":444},"obj":"CHEBI_25375"},{"id":"T202","span":{"begin":491,"end":498},"obj":"PATO_0002425"},{"id":"T203","span":{"begin":491,"end":498},"obj":"PATO_0001646"},{"id":"T204","span":{"begin":743,"end":748},"obj":"CHEBI_46629"},{"id":"T205","span":{"begin":743,"end":748},"obj":"CHEBI_15377"},{"id":"T206","span":{"begin":753,"end":757},"obj":"CHEBI_33290"}],"text":"Animals\nWfs1-deficient mice were generated by invalidating the 8th exon of the Wfs1 gene (for details, see Luuk et al., 2008). Experiments were performed in 3–4 months old male and female F2 hybrids [(129S6/SvEvTac × C57BL/6) × (129S6/SvEvTac × C57BL/6)]. Two separate batches of naïve animals were used for the FST and TST. For the gene expression measurements, naïve animals, taken directly from their home-cages, were used. For the monoamine measurement studies, both naïve mice and mice exposed to motility boxes were chosen. Breeding and genotyping were conducted in the Department of Physiology, University of Tartu. The animals were kept in groups of eight per cage at 22 ± 1°C in a room illuminated artificially from 7 am to 7 pm. Tap water and food pellets were freely available. The permission (No. 88, 25th of August, 2011) for the present study was given by the Estonian National Board of Animal Experiments in accordance with the European Communities Directive of 24 November 1986 (86/609/EEC). Behavioral experiments were carried out between 10:00 and 17:00. Wfs1-deficient homozygous mice were always used in parallel with their wild-type and heterozygous littermates and the animals were randomly divided into experimental groups."}