PMC:3724992 / 8637-9310 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"Induction of peptide-specific CTL with synthetic peptides\nBriefly, DCs were loaded with different peptides at a final concentration of 100 μg/ml for 4 h and were then irradiated with 20 Gy, which prevented all outgrowths in the control cultures. Autologous T cells were restimulated every 7 days with the previously mentioned peptide-pulsed DCs to generate peptide-specific CTLs. Recombinant interleukin 2 (IL-2) at a concentration of 20 U/ml was added to the culture medium on day 3 after every stimulation. Cytotoxic T lymphocyte activity was then assessed on day 23 by a 4 h 51Cr release assay. Effectors generated from negative peptide-pulsed DCs were used as controls.","divisions":[{"label":"title","span":{"begin":0,"end":57}}],"tracks":[]}