PMC:3724992 / 6001-6927 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"RT-PCR was used to analyze the expression of neuritin mRNA in cell lines. Total RNA was isolated from tumor cell lines using Tripure Isolation Regent Kit (Progema). Synthesis of cDNA was performed with 2 μg of total RNA with the aid of a reverse transcriptase Kit (Progema) and oligo(dT) primers. Two microliters RT product was amplified with PCR by using TaqDNA polymerase (Sangon, Shanghai) using standard procedures. The forward and reverse primer sequences were as follows: neuritin: sense primer, 5′-GTG CGA TGC AGT CTT TAA GTT-3′; anti-sense primer, 5′-GGG CTT TTC AGA CTG TTT GTT-3′; GAPDH: sense primer, 5′-GCA CCG TCA AGG CTG AGA AC-3′; antisense primer, 5′-ATG GTG GTG AAG ACG CCA GT-3′. Thirty amplification cycles were run: 1 min at 94 °C; 1 min at 60 °C; and 1 min at 72 °C. Cycling was ceased with a final extension of 10 min at 72 °C. RT-PCR products were then run on a gel and visualized with ethidium bromide.","tracks":[]}