PMC:3724992 / 5073-5960 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"Measurement of the peptide/HLA-A*0201 complex stability\nBriefly, T2 cells (106/ml) were incubated overnight with the candidate peptides, respectively, at a concentration of 20 μg/ml in serum-free medium supplemented with β2-microglobulin at a concentration of 3 μg/ml at 37 °C.\nThereafter, they were washed four times to remove free peptides, incubated with Brefeldin A (10 lg/ml) for 1 h to block cell surface expression of newly synthesized HLA-A2.1 molecules, washed and incubated at 37 °C for 0, 2, 4, 6, or 8 h. Subsequently, cells were stained with anti-HLA-A2 antibody from BB7.2 cells to evaluate the HLA-A2.1 molecule expression. For each time point, peptide induced HLA-A*0201 expression was evaluated by the formula mentioned above. Dissociation complex50 (DC50) was defined as the time required for the loss of 50 % of the HLA-A*0201/peptide complexes stabilized at time = 0.","divisions":[{"label":"title","span":{"begin":0,"end":55}},{"label":"p","span":{"begin":56,"end":277}}],"tracks":[]}