PMC:3724992 / 2480-3305 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"The human TAP-deficient T2 cell line, BB7.2 cell line producing mAb against HLA-A2, human glioma cell line U251 and U87 (HLA-A2+), human breast cancer cell line MCF-7 (HLA-A2+) were purchased from the American Type Culture Collection (Manassas, VA). Cells were cultured in RPMI-1640 medium containing 10 % FBS (Gibco, with endotoxin level ≤ 10 EU/ml), penicillin (200 U/ml), and streptomycin (100 μg/ml). All cell lines mentioned previously were kept at 37 °C in a humidified atmosphere containing 5 % CO2. HLA-A*0201/Kb transgenic (Tg) mice, 8–12 weeks-old, were purchased from The Jackson Laboratory (USA). Mice were bred and maintained in specific pathogen-free (SPF) facilities. Animal experiments were performed in accordance with the guidelines of the Animal Care and Use Committee of Third Military Medical University.","tracks":[]}