PMC:3724992 / 16531-17393 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"To determine whether the peptides induced effectors recognized target cells in an HLA-A2-restricted manner, the mAbs against HLA-A2 were used to block recognition by effectors. Our results showed that the anti-HLA-A2 antibody could significantly eliminate the cytotoxicity of the effectors against neuritin and HLA-A2 positive cells (Fig. 4), which implied that the induced effectors lysed target cells in an HLA-A2-restricted manner.\nFig. 4 Inhibited recognition of induced cells by anti-HLA-A2 antibody. Target cells were incubated with 100 μl anti-HLA-A2 antibody (functionally blocking mAb, from BB7.2 cell hybridoma supernate) for 1 h at 4 °C. The cytotoxic activities of CTLs were determined against target cells at various E/T ratios using 51Cr release assay. Experiments performed in triplicate showed consistent results. Compared with controls, P \u003c 0.05","divisions":[{"label":"label","span":{"begin":435,"end":441}}],"tracks":[]}