PMC:3724992 / 15637-16464 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"PBMCs from four HLA-A2.1+ donors were stimulated with synthetic peptides with the previously published method for CTL induction [19]. Of four tested, neuritin13–21, neuritin121–129 and neuritin4–12 peptides were able to elicit neuritin-specific CTLs, which could lyse target cells expressing neuritin and HLA-A2.1 (Fig. 3).\nFig. 3 Specific lysis of CTLs against target cells. Target cells were incubated with 51Cr (100 μCi per 1 × 106 cells) for 2 h in a 37 °C water bath. After incubation with 51Cr, target cells were washed three times with PBS, resuspended in RPMI-1640 medium, and mixed with effector cells at a 25:1, 50:1 or 100:1 of effector to target (E/T) ratio. After a 4-h incubation, the supernatants were harvested, and the amount of released 51Cr was measured with a gamma counter. Compared with controls, P \u003c 0.05","divisions":[{"label":"label","span":{"begin":324,"end":330}}],"tracks":[{"project":"2_test","denotations":[{"id":"23754640-18714399-63236740","span":{"begin":129,"end":131},"obj":"18714399"}],"attributes":[{"subj":"23754640-18714399-63236740","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93a1","default":true}]}]}}