PMC:3724992 / 13449-14329 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"Expression of neuritin in target cells\nThe expression of neuritin mRNA and protein in cell lines in this study was analyzed by RT-PCR and Western blot. As shown in Fig. 1, neuritin mRNA and protein were detected in U251 and U87 cell lines. However, neuritin mRNA and protein could not be detected in MCF-7 and autologous lymphocytes.\nFig. 1 Expression of neuritin in different target cells. Total RNA was isolated from tumor cell lines using Tripure Isolation Regent Kit. Two microliters RT product was amplified with PCR by using TaqDNA polymerase (using standard procedures). RT-PCR products were then run on a gel and visualized with ethidium bromide. For Western blot analysis, proteins in the cell extracts were separated by SDS-PAGE and were then analyzed with anti-neuritin MAb (antibodies-online company). 1 U251 cells; 2 U87 cells; 3 MCF-7 cells; 4 autologous lymphocytes","divisions":[{"label":"title","span":{"begin":0,"end":38}},{"label":"label","span":{"begin":334,"end":340}}],"tracks":[]}