PMC:3654953 / 11693-12315 JSONTXT

{"project":"2_test","denotations":[{"id":"23634874-23010432-81641078","span":{"begin":225,"end":227},"obj":"23010432"},{"id":"T59593","span":{"begin":225,"end":227},"obj":"23010432"}],"text":"Genomic DNA was extracted by standard methods from peripheral blood samples (I-2, II-2, II-4, II-6, II-7, III-1, III-3) and from skin fibroblasts (II-2, II-4). Whole-exome and Sanger’s sequencing were performed as described [11]. Total RNA was isolated from fibroblasts (RNeasy kit, Qiagen) and then transcribed to cDNA (Cloned AMV first-strand cDNA synthesis kit, Invitrogen). Quantitative Real-time PCR (QRtPCR) was assayed on an ABI Prism 7000 apparatus (Applied Biosystems). Additional file 2 reports primers and conditions for PCR amplifications of relevant exons of human GFAP and HDAC6 and for QRtPCR of HDAC6 cDNA."}