PMC:3625153 / 9947-11276 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3625153","sourcedb":"PMC","sourceid":"3625153","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3625153","text":"Expression of a mussel 5-HT1 gene product is up-regulated by 5-HT\nAs shown in Fig. 4A , a 1-hour incubation with 5-HT in vitro also induced a significant up-regulation of a transcript encoding a putative mussel 5-HT1 receptor (5-HT1) in haemocytes, while cell pre-treated with PROP significantly blocked the 5-HT effect on its receptor. In agreement, also haemocytes from mussels exposed in vivo to FX showed a significant up-regulation the 5-HT1 transcript ( Fig. 4B ). This effect was not observed in mussels exposed to the mixture FX+PROP ( Fig. 4B ).\n10.1371/journal.pone.0061634.g004 Figure 4 Relative mRNA levels of a 5-HT1 receptor are affected by 5-HT or FX exposure.\n(A) Relative 5-HT1 mRNA expression assessed in haemocytes incubated in vitro for 1 h with 1 µM 5-HT in the presence/absence of a 15-min pre-incubation with 100 µM PROP. Data are derived from three independent experiments (mean ± SEM); *p\u003c0.05 between pairs of samples. (B) Relative 5-HT1 mRNA expression evaluated in haemocytes of mussels exposed in vivo for 7 days to FX (0.3 ng/L), PROP (0.3 ng/L) or to the mixture FX+PROP (0.3 ng/L+0.3 ng/L). A group of control (water) exposed mussels were included in the analysis. Data are reported as mean ± SEM. Different superscripts letters indicate statistically significant differences (p\u003c0.05, N = 5).\n\nT","divisions":[{"label":"Title","span":{"begin":0,"end":65}},{"label":"Figure caption","span":{"begin":556,"end":1328}},{"label":"Title","span":{"begin":600,"end":677}}],"tracks":[]}