PMC:3625153 / 8082-9948 JSONTXT

{"project":"2_test","denotations":[{"id":"23593491-2888864-88440236","span":{"begin":1134,"end":1136},"obj":"2888864"},{"id":"23593491-8391422-88440237","span":{"begin":1140,"end":1142},"obj":"8391422"},{"id":"T27426","span":{"begin":1134,"end":1136},"obj":"2888864"},{"id":"T85711","span":{"begin":1140,"end":1142},"obj":"8391422"}],"text":"Expression of the mussel ABCB gene product is affected by serotoninergic but not by adrenergic agonists\nHaemocytes from control M. galloprovincialis were treated with either noradrenaline (NOR) or serotonin (5-HT) as selective agonists of adrenergic (AR) and 5-HT receptors, respectively. NOR did not increase either cAMP levels or PKA activities, whereas 5-HT addition significantly reduced both endpoints ( Fig. 2A,B ). Accordingly, ABCB mRNA expression was not affected by NOR while it was significantly down-regulated by 5-HT ( Fig. 2C ).\n10.1371/journal.pone.0061634.g002 Figure 2 Effects of NOR and 5-HT on cAMP-related parameters and ABCB mRNA expression in mussel haemocytes incubated in vitro.\n(A) cAMP content; (B) relative PKA activities; (C) relative ABCB mRNA expression. All biological endpoints were assessed after 1 h of exposure to 1 µM NOR or 5-HT. Data are derived from three independent experiments (mean ± SEM); *p\u003c0.05 vs control. Basal PKA activity = 1.12±0.17 nmol/min/mg protein. The effects of 5-HT on mussel haemocytes were further evaluated in the presence of PROP, used as a 5-HT1 receptor antagonist [26], [27]. PROP significantly blocked the reduction in cAMP levels and PKA activities and the down-regulation of the ABCB gene product triggered by 5-HT ( Fig. 3A,B,C ).\n10.1371/journal.pone.0061634.g003 Figure 3 Effects of PROP pre-incubation on 5-HT modulated parameters in mussel haemocytes incubated in vitro.\n(A) cAMP content; (B) relative PKA activities; (C) relative ABCB mRNA expression; (D) relative 5-HT1 mRNA expression. All biological endpoints were assessed after 1 h of exposure to 1 µM 5-HT in the presence/absence of a 15-min pre-incubation with 100 µM PROP. Data are derived from three independent experiments (mean ± SEM); *p\u003c0.05 between pairs of samples. Basal PKA activity  =  1.12±0.17 nmol/min/mg protein.\n\nE"}