PMC:3625153 / 30927-31395 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3625153","sourcedb":"PMC","sourceid":"3625153","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3625153","text":"PKA activity assay\nHaemocytes were lysed in cold PKA extraction buffer containing 25 mM Tris-HCl pH 7.4, 0.5 mM EDTA, 0.5 mM EGTA, 10 mM β-mercaptoethanol and 50-fold diluted proteinase inhibitor cocktail. Supernatants were assayed for PKA activity using the non-radioactive PepTag PKA assay kit with dye-labeled Kemptide as a substrate according to manufacturer's protocol. Results were normalized to the total protein content and expressed as fold change vs control.","divisions":[{"label":"Title","span":{"begin":0,"end":18}}],"tracks":[]}