PMC:3625153 / 30496-30925 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3625153","sourcedb":"PMC","sourceid":"3625153","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3625153","text":"cAMP assay\nHaemocytes were lysed in 65% ethanol for 1 h at 4°C. Lysates were centrifuged (2000×g for 15 min at 4°C), and the collected supernatants were dried under a stream of nitrogen. Contents of cAMP were assessed using the DetectX™ direct cyclic AMP enzyme immunoassay kit according to the manufacture's protocol. Total protein content was assessed using the Lowry method [55]. cAMP levels were expressed as pmol/mg protein.","divisions":[{"label":"Title","span":{"begin":0,"end":10}}],"tracks":[{"project":"2_test","denotations":[{"id":"23593491-14907713-88440278","span":{"begin":378,"end":380},"obj":"14907713"},{"id":"T51532","span":{"begin":378,"end":380},"obj":"14907713"}],"attributes":[{"subj":"23593491-14907713-88440278","pred":"source","obj":"2_test"},{"subj":"T51532","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93c4","default":true}]}]}}