PMC:3625153 / 13244-15529 JSONTXT

{"project":"2_test","denotations":[{"id":"23593491-8562304-88440238","span":{"begin":620,"end":622},"obj":"8562304"},{"id":"23593491-8562304-88440239","span":{"begin":1230,"end":1232},"obj":"8562304"},{"id":"23593491-8562304-88440240","span":{"begin":1401,"end":1403},"obj":"8562304"},{"id":"23593491-9458366-88440241","span":{"begin":1917,"end":1919},"obj":"9458366"},{"id":"T53271","span":{"begin":620,"end":622},"obj":"8562304"},{"id":"T79686","span":{"begin":1230,"end":1232},"obj":"8562304"},{"id":"T37090","span":{"begin":1401,"end":1403},"obj":"8562304"},{"id":"T72931","span":{"begin":1917,"end":1919},"obj":"9458366"}],"text":"Sequence analysis of the 5′-untranslated region of mussel ABCB genes\nThe computational analysis of the 5′-untranlasted regions of ABCB genes from two mytilid species M. galloproviancialis and M. californianus revealed the presence of several putative PKA-related regulatory elements within the ABCB promoter region ( Fig. 7 ). Amongst these, putative binding sites for the PKA-dependent CRE-BP transcription factor were found in both M. galloprovincialis (−76) and M. californianus (−96 and −170) ABCB promoter regions; binding sites for this transcription factor are also present within the human ABCB1 gene promoter ([28]; Fig. 7 ).\n10.1371/journal.pone.0061634.g007 Figure 7 Untranslated 5′ regulatory region of ABCB genes from the mussels M. galloprovincialis and M. californianus showing promoter elements.\nNumbering is relative to the transcription initiation site. Gene Bank Accession Numbers are FM999809 (M. galloprovincialis ABCB1) and EF52141 (M. californianus ABCB). Sequence of the human ABCB1 promoter in the same region is reported for comparison (GenBank Ac. Numb. NM000927). Furthermore, a more complete sequence analysis of the human ABCB1 promoter is reported by Scotto and Johnson [6] and Rohlff and Glazer [28]. Regulatory elements binding the AP-1 and AP-2 transcription factors, which occur in the human ABCB1 promoter and are regulated by PKA in a manner similar to CREB ([28] and reference therein), were also found in the 5′untranslated regions of both mussel ABCB gene sequences analysed ( Fig. 7 ). Moreover, binding sites for the Sp1 transcription factor were also found at position −35 (M. galloprovincialis), and at positions −55 and −177 (M. californianus) ( Fig. 7 ). Sp1 is a PKA-regulated nuclear transcription factor that binds to TATA-less promoters, including ABCB gene promoters, and it is primarily involved in constitutive rather than stress-induced gene regulation [6], [29]. Binding sites for the HSF transcription factor were found both in M. galloprovincialis (−95, −174) and M. californianus (−125) ABCB promoter regions ( Fig. 7 ). Finally, binding sites for SRF and NF-kB transcription factors were also found in ABCB promoter regions from both mussel species ( Fig. 7 ), consistent with reports for the human ABCB gene promoter [6]."}