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{"project":"GO-CC","denotations":[{"id":"T3595","span":{"begin":64,"end":69},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"sentences","denotations":[{"id":"T3582","span":{"begin":71,"end":371},"obj":"Sentence"},{"id":"T3581","span":{"begin":0,"end":70},"obj":"Sentence"},{"id":"T43","span":{"begin":0,"end":70},"obj":"Sentence"},{"id":"T44","span":{"begin":71,"end":371},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"simple1","denotations":[{"id":"T3603","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3602","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3601","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3600","span":{"begin":265,"end":273},"obj":"Protein"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T3678","span":{"begin":238,"end":248},"obj":"Gene_expression"},{"id":"T3677","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3676","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3675","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3674","span":{"begin":265,"end":273},"obj":"Protein"}],"relations":[{"id":"R2694","pred":"themeOf","subj":"T3674","obj":"T3678"},{"id":"R2695","pred":"themeOf","subj":"T3675","obj":"T3678"},{"id":"R2696","pred":"themeOf","subj":"T3676","obj":"T3678"},{"id":"R2697","pred":"themeOf","subj":"T3677","obj":"T3678"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T3692","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3691","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3690","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3689","span":{"begin":265,"end":273},"obj":"Protein"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"DLUT931","denotations":[{"id":"T3684","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3683","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3682","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3681","span":{"begin":265,"end":273},"obj":"Protein"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T3719","span":{"begin":225,"end":260},"obj":"Gene_expression"},{"id":"T3718","span":{"begin":59,"end":69},"obj":"Entity"},{"id":"T3717","span":{"begin":99,"end":108},"obj":"Protein"},{"id":"T3716","span":{"begin":49,"end":58},"obj":"Protein"},{"id":"T3713","span":{"begin":300,"end":321},"obj":"Protein"},{"id":"T3712","span":{"begin":11,"end":28},"obj":"Protein"},{"id":"T3711","span":{"begin":326,"end":349},"obj":"Protein"},{"id":"T3710","span":{"begin":225,"end":237},"obj":"Protein"},{"id":"T3709","span":{"begin":139,"end":198},"obj":"Entity"},{"id":"T3708","span":{"begin":75,"end":89},"obj":"Entity"},{"id":"T3707","span":{"begin":265,"end":299},"obj":"Protein"}],"relations":[{"id":"R2698","pred":"themeOf","subj":"T3710","obj":"T3719"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T3699","span":{"begin":335,"end":348},"obj":"Protein"},{"id":"T3698","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3697","span":{"begin":307,"end":320},"obj":"Protein"},{"id":"T3696","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3695","span":{"begin":284,"end":297},"obj":"Protein"},{"id":"T3694","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3693","span":{"begin":265,"end":273},"obj":"Protein"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"testone","denotations":[{"id":"T3571","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3570","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3569","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3568","span":{"begin":265,"end":273},"obj":"Protein"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"test3","denotations":[{"id":"T3579","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3578","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3577","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3576","span":{"begin":265,"end":273},"obj":"Protein"},{"id":"T3575","span":{"begin":326,"end":333},"obj":"Protein"},{"id":"T3574","span":{"begin":300,"end":305},"obj":"Protein"},{"id":"T3573","span":{"begin":274,"end":282},"obj":"Protein"},{"id":"T3572","span":{"begin":265,"end":273},"obj":"Protein"}],"text":"The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}