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{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T3590","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3589","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3588","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3587","span":{"begin":299,"end":307},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T3680","span":{"begin":360,"end":367},"obj":"http://www.uniprot.org/uniprot/P60709"},{"id":"T3679","span":{"begin":334,"end":339},"obj":"http://www.uniprot.org/uniprot/P28033"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"GO-BP","denotations":[{"id":"T3594","span":{"begin":267,"end":282},"obj":"http://purl.obolibrary.org/obo/GO_0010467"},{"id":"T3593","span":{"begin":189,"end":202},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T3592","span":{"begin":181,"end":202},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T3591","span":{"begin":114,"end":123},"obj":"http://purl.obolibrary.org/obo/GO_0009058"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"GO-CC","denotations":[{"id":"T3595","span":{"begin":98,"end":103},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"sentences","denotations":[{"id":"T3582","span":{"begin":105,"end":405},"obj":"Sentence"},{"id":"T3581","span":{"begin":34,"end":104},"obj":"Sentence"},{"id":"T3580","span":{"begin":0,"end":33},"obj":"Sentence"},{"id":"T42","span":{"begin":0,"end":33},"obj":"Sentence"},{"id":"T43","span":{"begin":34,"end":104},"obj":"Sentence"},{"id":"T44","span":{"begin":105,"end":405},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"simple1","denotations":[{"id":"T3603","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3602","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3601","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3600","span":{"begin":299,"end":307},"obj":"Protein"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T3678","span":{"begin":272,"end":282},"obj":"Gene_expression"},{"id":"T3677","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3676","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3675","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3674","span":{"begin":299,"end":307},"obj":"Protein"}],"relations":[{"id":"R2694","pred":"themeOf","subj":"T3674","obj":"T3678"},{"id":"R2695","pred":"themeOf","subj":"T3675","obj":"T3678"},{"id":"R2696","pred":"themeOf","subj":"T3676","obj":"T3678"},{"id":"R2697","pred":"themeOf","subj":"T3677","obj":"T3678"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T3692","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3691","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3690","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3689","span":{"begin":299,"end":307},"obj":"Protein"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"DLUT931","denotations":[{"id":"T3684","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3683","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3682","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3681","span":{"begin":299,"end":307},"obj":"Protein"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T3719","span":{"begin":259,"end":294},"obj":"Gene_expression"},{"id":"T3718","span":{"begin":93,"end":103},"obj":"Entity"},{"id":"T3717","span":{"begin":133,"end":142},"obj":"Protein"},{"id":"T3716","span":{"begin":83,"end":92},"obj":"Protein"},{"id":"T3715","span":{"begin":28,"end":32},"obj":"Protein"},{"id":"T3714","span":{"begin":0,"end":33},"obj":"Protein"},{"id":"T3713","span":{"begin":334,"end":355},"obj":"Protein"},{"id":"T3712","span":{"begin":45,"end":62},"obj":"Protein"},{"id":"T3711","span":{"begin":360,"end":383},"obj":"Protein"},{"id":"T3710","span":{"begin":259,"end":271},"obj":"Protein"},{"id":"T3709","span":{"begin":173,"end":232},"obj":"Entity"},{"id":"T3708","span":{"begin":109,"end":123},"obj":"Entity"},{"id":"T3707","span":{"begin":299,"end":333},"obj":"Protein"}],"relations":[{"id":"R2698","pred":"themeOf","subj":"T3710","obj":"T3719"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T3699","span":{"begin":369,"end":382},"obj":"Protein"},{"id":"T3698","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3697","span":{"begin":341,"end":354},"obj":"Protein"},{"id":"T3696","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3695","span":{"begin":318,"end":331},"obj":"Protein"},{"id":"T3694","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3693","span":{"begin":299,"end":307},"obj":"Protein"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"testone","denotations":[{"id":"T3571","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3570","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3569","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3568","span":{"begin":299,"end":307},"obj":"Protein"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}

{"project":"test3","denotations":[{"id":"T3579","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3578","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3577","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3576","span":{"begin":299,"end":307},"obj":"Protein"},{"id":"T3575","span":{"begin":360,"end":367},"obj":"Protein"},{"id":"T3574","span":{"begin":334,"end":339},"obj":"Protein"},{"id":"T3573","span":{"begin":308,"end":316},"obj":"Protein"},{"id":"T3572","span":{"begin":299,"end":307},"obj":"Protein"}],"text":"Real-time Quantitative PCR (qPCR)\nThe RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells. For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems)."}