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PMC:3589482 / 5913-8488 JSONTXT

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bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4551 2571-2572 -RRB- denotes )
T4550 2561-2571 NNP denotes Invitrogen
T4549 2560-2561 -LRB- denotes (
T4548 2555-2559 NNS denotes gels
T4547 2548-2554 NNP denotes NuPAGE
T4546 2539-2547 NNP denotes Bis-Tris
T4545 2537-2538 NN denotes %
T4544 2535-2537 CD denotes 12
T4543 2533-2534 CD denotes 4
T4542 2530-2532 IN denotes on
T4541 2520-2529 VBN denotes separated
T4540 2515-2519 VBD denotes were
T4539 2506-2514 NNS denotes proteins
T4538 2504-2505 , denotes ,
T4537 2503-2504 -RRB- denotes )
T4536 2500-2503 NNP denotes SDS
T4535 2498-2499 NN denotes %
T4534 2495-2498 CD denotes 0.1
T4533 2493-2494 , denotes ,
T4532 2481-2493 NN denotes deoxycholate
T4531 2479-2480 NN denotes %
T4530 2476-2479 CD denotes 0.5
T4529 2474-2475 , denotes ,
T4528 2469-2474 NN denotes NP-40
T4527 2467-2468 NN denotes %
T4526 2464-2467 CD denotes 0.5
T4525 2462-2463 , denotes ,
T4524 2457-2462 NNP denotes X-100
T4523 2450-2456 NNP denotes Triton
T4522 2449-2450 NN denotes %
T4521 2448-2449 CD denotes 1
T4520 2446-2447 , denotes ,
T4519 2442-2446 NNP denotes NaCl
T4518 2439-2441 NNP denotes mM
T4517 2435-2438 CD denotes 150
T4516 2433-2434 , denotes ,
T4515 2429-2433 NNP denotes Tris
T4514 2426-2428 NNP denotes mM
T4513 2423-2425 CD denotes 10
T4512 2422-2423 -LRB- denotes (
T4511 2415-2421 NN denotes buffer
T4510 2410-2414 NNP denotes RIPA
T4509 2407-2409 IN denotes in
T4508 2398-2406 VBN denotes prepared
T4239 2351-2352 . denotes .
T4238 2343-2351 CD denotes AF339731
T4237 2341-2342 . denotes .
T4236 2339-2341 NNP denotes No
T4235 2329-2338 NNP denotes Accession
T4234 2324-2328 IN denotes with
T4233 2319-2323 NNP denotes Bank
T4232 2314-2318 NNP denotes Data
T4231 2296-2313 NNP denotes GenBank/EMBL/DDBJ
T4230 2293-2295 IN denotes in
T4229 2283-2292 VBN denotes deposited
T4228 2279-2282 VBD denotes was
T4227 2270-2278 NN denotes sequence
T4226 2265-2269 DT denotes This
T4225 2263-2264 . denotes .
T4224 2262-2263 -RRB- denotes )
T4223 2250-2262 NNP denotes Perkin-Elmer
T4222 2249-2250 -LRB- denotes (
T4221 2239-2248 NN denotes sequencer
T4220 2234-2238 NNP denotes 373A
T4219 2231-2233 NNP denotes PE
T4218 2229-2230 DT denotes a
T4217 2225-2228 CC denotes and
T4216 2223-2224 -RRB- denotes )
T4215 2211-2223 NNP denotes Perkin-Elmer
T4214 2210-2211 -LRB- denotes (
T4213 2206-2209 NN denotes kit
T4212 2197-2205 NN denotes reaction
T4211 2191-2196 JJ denotes ready
T4210 2180-2190 VBG denotes sequencing
T4209 2174-2179 NN denotes cycle
T4208 2163-2173 NN denotes terminator
T4207 2159-2162 NN denotes dye
T4206 2153-2158 NNP denotes PRISM
T4205 2149-2152 NNP denotes ABI
T4204 2145-2148 DT denotes the
T4203 2139-2144 VBG denotes using
T4202 2128-2138 VBN denotes determined
T4201 2124-2127 VBD denotes was
T4200 2117-2123 NN denotes region
T4199 2108-2116 NN denotes flanking
T4198 2106-2107 NN denotes ′
T4197 2105-2106 CD denotes 5
T4196 2100-2104 NN denotes gene
T4195 2091-2099 NNP denotes SerpinB2
T4194 2084-2090 NN denotes murine
T4193 2081-2083 NN denotes bp
T4192 2076-2080 CD denotes 4480
T4191 2072-2075 DT denotes the
T4190 2069-2071 IN denotes of
T4189 2060-2068 NN denotes sequence
T4188 2049-2059 NN denotes nucleotide
T4187 2045-2048 DT denotes The
T4186 2043-2044 . denotes .
T4185 2033-2043 NN denotes sequencing
T4184 2029-2032 NN denotes DNA
T4183 2025-2028 CC denotes and
T4182 2015-2024 NN denotes digestion
T4181 2008-2014 NN denotes enzyme
T4180 1996-2007 NN denotes restriction
T4179 1993-1995 IN denotes by
T4178 1984-1992 VBN denotes verified
T4177 1979-1983 VBD denotes were
T4176 1971-1978 NNS denotes inserts
T4175 1961-1970 VBN denotes Subcloned
T4174 1959-1960 . denotes .
T4173 1947-1959 NNS denotes orientations
T4172 1938-1946 JJ denotes opposite
T4171 1935-1937 IN denotes in
T4170 1928-1934 VBN denotes cloned
T4169 1926-1927 , denotes ,
T4168 1918-1926 NN denotes fragment
T4167 1912-1917 NNP denotes EcoRI
T4166 1904-1911 NNP denotes pDB9406
T4165 1900-1903 DT denotes the
T4164 1897-1899 IN denotes of
T4163 1888-1896 RB denotes upstream
T4162 1876-1887 RB denotes immediately
T4161 1868-1875 VBN denotes located
T4160 1866-1867 , denotes ,
T4159 1858-1866 NN denotes fragment
T4158 1850-1857 JJ denotes genomic
T4157 1844-1849 NNP denotes EcoRI
T4156 1841-1843 NN denotes kb
T4155 1837-1840 CD denotes 1.2
T4154 1835-1836 DT denotes a
T4153 1827-1834 VBP denotes contain
T4152 1816-1826 NN denotes pDB9402-42
T4151 1812-1815 CC denotes and
T4150 1801-1811 NN denotes pDB9402-41
T4149 1799-1800 : denotes ;
T4148 1795-1799 NN denotes site
T4147 1784-1794 NN denotes initiation
T4146 1770-1783 NN denotes transcription
T4145 1766-1769 DT denotes the
T4144 1757-1765 VBG denotes spanning
T4143 1748-1756 NN denotes fragment
T4142 1740-1747 JJ denotes genomic
T4141 1729-1739 NNP denotes EcoRI/SpeI
T4140 1726-1728 NN denotes kb
T4139 1722-1725 CD denotes 4.4
T4138 1720-1721 DT denotes a
T4137 1711-1719 VBZ denotes contains
T4136 1703-1710 JJ denotes pDB9406
T4135 1701-1702 . denotes .
T4134 1695-1701 NNP denotes Geneva
T4133 1692-1694 IN denotes of
T4132 1681-1691 NNP denotes University
T4131 1679-1680 , denotes ,
T4130 1674-1679 NNP denotes Belin
T4129 1664-1673 NNP denotes Dominique
T4128 1660-1663 NNP denotes Dr.
T4127 1657-1659 IN denotes by
T4126 1648-1656 VBN denotes provided
T4125 1641-1647 RB denotes kindly
T4124 1636-1640 VBD denotes were
T4123 1634-1635 , denotes ,
T4122 1628-1634 NN denotes strain
T4121 1622-1627 NN denotes mouse
T4552 2572-2573 , denotes ,
T4120 1618-1621 CD denotes 129
T4119 1616-1617 DT denotes a
T4118 1611-1615 IN denotes from
T4117 1602-1610 VBN denotes prepared
T4116 1594-1601 NN denotes library
T4115 1586-1593 JJ denotes genomic
T4114 1584-1585 -RRB- denotes )
T4113 1574-1584 NNP denotes Stratagene
T4112 1573-1574 -LRB- denotes (
T4111 1566-1572 NNP denotes λFIXII
T4110 1564-1565 DT denotes a
T4109 1559-1563 IN denotes from
T4108 1550-1558 VBD denotes isolated
T4107 1546-1549 NNP denotes DNA
T4106 1538-1545 JJ denotes genomic
T4105 1527-1537 VBG denotes containing
T4104 1516-1526 NNP denotes pDB9402-42
T4103 1512-1515 CC denotes and
T4102 1501-1511 NNP denotes pDB9402-41
T4101 1499-1500 , denotes ,
T4100 1492-1499 NNP denotes pDB9406
T4099 1483-1491 NNP denotes Plasmids
T4098 1481-1482 . denotes .
T4097 1472-1481 NN denotes digestion
T4096 1468-1471 NNP denotes III
T4095 1456-1467 NN denotes exonuclease
T4063 1228-1236 NN denotes sequence
T4062 1224-1227 NNP denotes DNA
T4061 1220-1223 NNP denotes The
T4060 1211-1219 NNP denotes Analysis
T4059 1202-1210 NNP denotes Sequence
T3794 1195-1196 . denotes .
T3793 1194-1195 -RRB- denotes )
T3792 1184-1194 NNPS denotes Biosystems
T3791 1176-1183 NNP denotes Applied
T3790 1175-1176 -LRB- denotes (
T3789 1173-1174 -RRB- denotes )
T3788 1160-1173 NNP denotes Mm00607939_s1
T3787 1159-1160 -LRB- denotes (
T3786 1151-1158 JJ denotes β-actin
T3785 1147-1150 CC denotes and
T3784 1145-1146 -RRB- denotes )
T3783 1132-1145 NNP denotes Mm00843434_s1
T3782 1131-1132 -LRB- denotes (
T3781 1125-1130 NNP denotes Cebpb
T3780 1123-1124 , denotes ,
T3779 1122-1123 -RRB- denotes )
T3778 1109-1122 NNP denotes Mm00440905_m1
T3777 1108-1109 -LRB- denotes (
T3776 1090-1107 NNP denotes Serpinb2/SerpinB2
T3775 1086-1089 IN denotes for
T3774 1078-1085 NNS denotes primers
T3773 1074-1077 NNP denotes 20X
T3772 1063-1073 NNP denotes Expression
T3771 1058-1062 NNP denotes Gene
T3770 1056-1057 NNP denotes ®
T3769 1050-1056 NNP denotes TaqMan
T3768 1044-1049 VBG denotes using
T3767 1034-1043 VBN denotes performed
T3766 1030-1033 VBD denotes was
T3765 1025-1029 NNP denotes qPCR
T3764 1023-1024 . denotes .
T3763 1022-1023 -RRB- denotes )
T3762 1012-1022 NNPS denotes Biosystems
T3761 1004-1011 NNP denotes Applied
T3760 1003-1004 -LRB- denotes (
T3759 994-1002 NNP denotes Reagents
T3758 980-993 NNP denotes Transcription
T3757 972-979 VB denotes Reverse
T3756 970-971 NNP denotes ®
T3755 964-970 NNP denotes TaqMan
T3754 958-963 VBG denotes using
T3753 946-957 VBN denotes transcribed
T3752 938-945 JJ denotes reverse
T3751 934-937 VBD denotes was
T3750 930-933 NNP denotes RNA
T3749 924-929 JJ denotes total
T3748 921-923 IN denotes of
T3747 918-920 NN denotes µg
T3746 916-917 CD denotes 1
T3745 914-915 , denotes ,
T3744 905-914 NN denotes synthesis
T3743 900-904 NNP denotes cDNA
T3742 896-899 IN denotes For
T3741 894-895 . denotes .
T3740 889-894 NNS denotes cells
T3739 884-888 IN denotes from
T3738 880-883 NNP denotes RNA
T3737 874-879 JJ denotes total
T3736 866-873 VB denotes isolate
T3735 863-865 TO denotes to
T3734 858-862 VBN denotes used
T3733 854-857 VBD denotes was
T3732 852-853 -RRB- denotes )
T3731 846-852 NNP denotes Qiagen
T3730 845-846 -LRB- denotes (
T3729 841-844 NNP denotes Kit
T3728 836-840 NNP denotes Mini
T3727 829-835 NNP denotes RNeasy
T3726 825-828 DT denotes The
T3725 823-824 -RRB- denotes )
T3724 819-823 NNP denotes qPCR
T3723 818-819 -LRB- denotes (
T3722 814-817 NNP denotes PCR
T3721 801-813 JJ denotes Quantitative
T3720 791-800 JJ denotes Real-time
T4094 1453-1455 IN denotes by
T4093 1442-1452 VBN denotes introduced
T4092 1432-1441 NNS denotes deletions
T4091 1421-1431 VBG denotes containing
T4090 1410-1420 NN denotes pDB9402-42
T4089 1406-1409 CC denotes and
T4088 1395-1405 NN denotes pDB9402-41
T4087 1390-1394 IN denotes from
T4086 1381-1389 VBN denotes prepared
T4085 1372-1380 NNS denotes plasmids
T4084 1369-1371 TO denotes to
T4083 1360-1368 NN denotes addition
T4082 1357-1359 IN denotes in
T4081 1355-1356 , denotes ,
T4080 1345-1355 NNP denotes pDB9402-42
T4079 1341-1344 CC denotes and
T4078 1330-1340 NNP denotes pDB9402-41
T4077 1328-1329 , denotes ,
T4076 1321-1328 NNP denotes pDB9406
T4075 1312-1320 NNS denotes plasmids
T4074 1302-1311 JJ denotes pUC-based
T4073 1298-1301 DT denotes the
T4072 1287-1297 VBG denotes sequencing
T4071 1284-1286 IN denotes by
T4070 1273-1283 VBN denotes determined
T4069 1269-1272 VBD denotes was
T4068 1260-1268 NN denotes promoter
T4067 1251-1259 NNP denotes SerpinB2
T4066 1244-1250 NN denotes murine
T4065 1240-1243 DT denotes the
T4064 1237-1239 IN denotes of
T3561 788-789 . denotes .
T3560 783-788 NNP denotes Sigma
T3559 778-782 IN denotes from
T3558 769-777 VBN denotes obtained
T3557 765-768 VBD denotes was
T3556 763-764 -RRB- denotes )
T3555 758-763 NNP denotes Re595
T3554 751-757 NNP denotes Strain
T3553 741-750 NN denotes minnesota
T3552 730-740 NN denotes Salmonella
T3551 729-730 -LRB- denotes (
T3550 727-728 -RRB- denotes )
T3549 724-727 NNP denotes LPS
T3548 723-724 -LRB- denotes (
T3547 704-722 NN denotes lipopolysaccharide
T3546 694-703 JJ denotes Bacterial
T3545 692-693 . denotes .
T3544 691-692 -RRB- denotes )
T3543 686-691 NNP denotes Lonza
T3542 685-686 -LRB- denotes (
T3541 681-684 NNP denotes Kit
T3540 671-680 NNP denotes Detection
T3539 661-670 NNP denotes MycoAlert
T3538 657-660 DT denotes the
T3537 653-656 CC denotes and
T3536 651-652 -RRB- denotes )
T3535 646-651 NNP denotes Sigma
T3534 645-646 -LRB- denotes (
T3533 639-644 CD denotes 33258
T3532 633-638 VB denotes stain
T3531 625-632 NNP denotes Hoechst
T3530 619-624 VBG denotes using
T3529 610-618 NN denotes staining
T3528 602-609 JJ denotes nuclear
T3527 599-601 IN denotes by
T3526 589-598 NN denotes infection
T3525 578-588 NNP denotes Mycoplasma
T3524 570-577 VB denotes exclude
T3523 567-569 TO denotes to
T3522 559-566 VBN denotes checked
T3521 549-558 RB denotes routinely
T3520 544-548 VBD denotes were
T3519 535-543 NNS denotes cultures
T3518 531-534 DT denotes All
T3517 529-530 . denotes .
T3516 523-529 NN denotes method
T3515 513-522 NN denotes exclusion
T3514 509-512 NN denotes dye
T3513 507-508 -RRB- denotes )
T3512 502-507 NNP denotes Sigma
T3511 501-502 -LRB- denotes (
T3477 285-290 NNS denotes media
T3476 276-284 CD denotes DMEM/F12
T3475 274-275 NN denotes %
T3474 272-274 CD denotes 50
T3473 269-271 IN denotes in
T3472 258-268 VBD denotes maintained
T3471 254-257 CC denotes and
T3470 252-253 , denotes ,
T3469 247-252 RB denotes later
T3468 242-246 NNS denotes days
T3467 240-241 CD denotes 5
T3466 238-239 CD denotes 3
T3465 231-237 NN denotes lavage
T3464 220-230 JJ denotes peritoneal
T3463 217-219 IN denotes by
T3462 208-216 VBN denotes followed
T3461 202-207 NN denotes broth
T3460 187-201 NN denotes thioglycollate
T3459 185-186 NN denotes %
T3458 184-185 CD denotes 3
T3457 179-183 IN denotes with
T3456 174-178 NNS denotes mice
T3455 166-173 NNP denotes C57BL/6
T3454 156-165 VBG denotes injecting
T3453 153-155 IN denotes by
T3452 144-152 VBN denotes obtained
T3451 139-143 VBD denotes were
T3450 127-138 NNS denotes macrophages
T3449 116-126 JJ denotes peritoneal
T3448 108-115 JJ denotes Primary
T3447 106-107 . denotes .
T3446 105-106 -RRB- denotes )
T3445 98-105 NNP denotes Cellgro
T3444 97-98 -LRB- denotes (
T3443 88-96 NN denotes solution
T3442 54-87 JJ denotes penicillin/streptomycin/glutamate
T3441 52-53 NN denotes %
T3440 51-52 CD denotes 1
T3439 47-50 CC denotes and
T3438 41-46 NN denotes serum
T3437 34-40 JJ denotes bovine
T3436 28-33 JJ denotes fetal
T3435 26-27 NN denotes %
T3434 24-26 CD denotes 10
T3433 19-23 IN denotes with
T3432 6-18 VBN denotes supplemented
T3431 4-5 -RRB- denotes )
T3510 496-500 NN denotes blue
T3509 489-495 NN denotes trypan
T3508 485-488 DT denotes the
T3507 479-484 VBG denotes using
T3506 468-478 VBN denotes determined
T3505 464-467 VBD denotes was
T3504 454-463 NN denotes viability
T3503 449-453 NNP denotes Cell
T3502 447-448 . denotes .
T3501 442-447 NN denotes serum
T3500 433-441 JJ denotes LPS-free
T3499 423-432 VBN denotes certified
T3498 420-422 IN denotes of
T3497 416-419 NN denotes use
T3496 412-415 DT denotes the
T3495 404-411 IN denotes through
T3494 395-403 NNS denotes cultures
T3493 390-394 NN denotes cell
T3492 386-389 DT denotes all
T3491 381-385 IN denotes from
T3490 367-380 NN denotes contamination
T3489 348-366 NN denotes lipopolysaccharide
T3488 338-347 JJ denotes bacterial
T3487 330-337 VB denotes exclude
T3486 327-329 TO denotes to
T3481 301-302 -RRB- denotes )
T3480 298-301 NNP denotes BRL
T3479 292-297 NNP denotes Gibco
T3478 291-292 -LRB- denotes (
T4507 2393-2397 VBD denotes were
T4506 2385-2392 NNS denotes lysates
T4505 2380-2384 NN denotes cell
T4504 2374-2379 NNP denotes Whole
T4503 2367-2373 NNP denotes Assays
T4502 2362-2366 NNP denotes Blot
T4501 2354-2361 JJ denotes Western
T4058 1198-1201 NNP denotes DNA
T3485 321-326 VBN denotes taken
T3484 316-320 VBD denotes were
T3483 304-315 NNS denotes Precautions
T3482 302-303 . denotes .
R2493 T3433 T3432 prep with,supplemented
R2494 T3434 T3435 nummod 10,%
R2495 T3435 T3438 nmod %,serum
R2496 T3436 T3438 amod fetal,serum
R2497 T3437 T3438 amod bovine,serum
R2498 T3438 T3433 pobj serum,with
R2499 T3439 T3438 cc and,serum
R2500 T3440 T3441 nummod 1,%
R2501 T3441 T3443 nmod %,solution
R2502 T3442 T3443 amod penicillin/streptomycin/glutamate,solution
R2503 T3443 T3438 conj solution,serum
R2504 T3444 T3445 punct (,Cellgro
R2505 T3445 T3443 appos Cellgro,solution
R2506 T3446 T3445 punct ),Cellgro
R2508 T3448 T3450 amod Primary,macrophages
R2509 T3449 T3450 amod peritoneal,macrophages
R2510 T3450 T3452 nsubjpass macrophages,obtained
R2511 T3451 T3452 auxpass were,obtained
R2512 T3452 T3452 ROOT obtained,obtained
R2513 T3453 T3452 prep by,obtained
R2514 T3454 T3453 pcomp injecting,by
R2515 T3455 T3456 compound C57BL/6,mice
R2516 T3456 T3454 dobj mice,injecting
R2517 T3457 T3454 prep with,injecting
R2518 T3458 T3459 nummod 3,%
R2519 T3459 T3461 nmod %,broth
R2520 T3460 T3461 compound thioglycollate,broth
R2521 T3461 T3457 pobj broth,with
R2522 T3462 T3461 acl followed,broth
R2523 T3463 T3462 agent by,followed
R2524 T3464 T3465 amod peritoneal,lavage
R2525 T3465 T3463 pobj lavage,by
R2526 T3466 T3465 nummod 3,lavage
R2527 T3467 T3468 nummod 5,days
R2528 T3468 T3469 npadvmod days,later
R2529 T3469 T3462 advmod later,followed
R2530 T3470 T3452 punct ",",obtained
R2531 T3471 T3452 cc and,obtained
R2532 T3472 T3452 conj maintained,obtained
R2533 T3473 T3472 prep in,maintained
R2534 T3474 T3475 nummod 50,%
R2535 T3475 T3473 pobj %,in
R2536 T3476 T3477 nummod DMEM/F12,media
R2537 T3477 T3473 pobj media,in
R2538 T3478 T3480 punct (,BRL
R2539 T3479 T3480 compound Gibco,BRL
R2540 T3480 T3472 appos BRL,maintained
R2541 T3481 T3480 punct ),BRL
R2542 T3482 T3452 punct .,obtained
R2543 T3483 T3485 nsubjpass Precautions,taken
R2544 T3484 T3485 auxpass were,taken
R2545 T3485 T3485 ROOT taken,taken
R2546 T3486 T3487 aux to,exclude
R2547 T3487 T3485 advcl exclude,taken
R2548 T3488 T3490 amod bacterial,contamination
R2549 T3489 T3490 compound lipopolysaccharide,contamination
R2550 T3490 T3487 dobj contamination,exclude
R2551 T3491 T3490 prep from,contamination
R2552 T3492 T3494 det all,cultures
R2553 T3493 T3494 compound cell,cultures
R2554 T3494 T3491 pobj cultures,from
R2555 T3495 T3487 prep through,exclude
R2556 T3496 T3497 det the,use
R2557 T3497 T3495 pobj use,through
R2558 T3498 T3497 prep of,use
R2559 T3499 T3501 amod certified,serum
R2560 T3500 T3501 amod LPS-free,serum
R2561 T3501 T3498 pobj serum,of
R2562 T3502 T3485 punct .,taken
R2563 T3503 T3504 compound Cell,viability
R2564 T3504 T3506 nsubjpass viability,determined
R2565 T3505 T3506 auxpass was,determined
R2566 T3506 T3506 ROOT determined,determined
R2567 T3507 T3506 advcl using,determined
R2568 T3508 T3510 det the,blue
R2569 T3509 T3510 compound trypan,blue
R2570 T3510 T3516 nmod blue,method
R2571 T3511 T3510 punct (,blue
R2572 T3512 T3510 appos Sigma,blue
R2573 T3513 T3510 punct ),blue
R2574 T3514 T3515 compound dye,exclusion
R2575 T3515 T3516 compound exclusion,method
R2576 T3516 T3507 dobj method,using
R2577 T3517 T3506 punct .,determined
R2578 T3518 T3519 det All,cultures
R2579 T3519 T3522 nsubjpass cultures,checked
R2580 T3520 T3522 auxpass were,checked
R2581 T3521 T3522 advmod routinely,checked
R2582 T3522 T3522 ROOT checked,checked
R2583 T3523 T3524 aux to,exclude
R2584 T3524 T3522 xcomp exclude,checked
R2585 T3525 T3526 compound Mycoplasma,infection
R2586 T3526 T3524 dobj infection,exclude
R2587 T3527 T3526 prep by,infection
R2588 T3528 T3529 amod nuclear,staining
R2589 T3529 T3527 pobj staining,by
R2590 T3530 T3524 advcl using,exclude
R2591 T3531 T3532 nsubj Hoechst,stain
R2592 T3532 T3530 xcomp stain,using
R2593 T3533 T3535 nummod 33258,Sigma
R2594 T3534 T3535 punct (,Sigma
R2595 T3535 T3524 npadvmod Sigma,exclude
R2596 T3536 T3535 punct ),Sigma
R2597 T3537 T3535 cc and,Sigma
R2598 T3538 T3541 det the,Kit
R2599 T3539 T3541 compound MycoAlert,Kit
R2600 T3540 T3541 compound Detection,Kit
R2601 T3541 T3535 conj Kit,Sigma
R2602 T3542 T3541 punct (,Kit
R2603 T3543 T3541 appos Lonza,Kit
R2604 T3544 T3541 punct ),Kit
R2605 T3545 T3522 punct .,checked
R2606 T3546 T3547 amod Bacterial,lipopolysaccharide
R2607 T3547 T3558 nsubjpass lipopolysaccharide,obtained
R2608 T3548 T3549 punct (,LPS
R2609 T3549 T3547 appos LPS,lipopolysaccharide
R2610 T3550 T3547 punct ),lipopolysaccharide
R2611 T3551 T3555 punct (,Re595
R2612 T3552 T3555 compound Salmonella,Re595
R2613 T3553 T3555 compound minnesota,Re595
R2614 T3554 T3555 compound Strain,Re595
R2615 T3555 T3547 appos Re595,lipopolysaccharide
R2616 T3556 T3555 punct ),Re595
R2617 T3557 T3558 auxpass was,obtained
R2618 T3558 T3558 ROOT obtained,obtained
R2619 T3559 T3558 prep from,obtained
R2620 T3560 T3559 pobj Sigma,from
R2621 T3561 T3558 punct .,obtained
R2699 T3720 T3722 amod Real-time,PCR
R2700 T3721 T3722 compound Quantitative,PCR
R2701 T3722 T3722 ROOT PCR,PCR
R2702 T3723 T3724 punct (,qPCR
R2703 T3724 T3722 appos qPCR,PCR
R2704 T3725 T3722 punct ),PCR
R2705 T3726 T3729 det The,Kit
R2706 T3727 T3729 compound RNeasy,Kit
R2707 T3728 T3729 compound Mini,Kit
R2708 T3729 T3734 nsubjpass Kit,used
R2709 T3730 T3729 punct (,Kit
R2710 T3731 T3729 appos Qiagen,Kit
R2711 T3732 T3729 punct ),Kit
R2712 T3733 T3734 auxpass was,used
R2713 T3734 T3734 ROOT used,used
R2714 T3735 T3736 aux to,isolate
R2715 T3736 T3734 xcomp isolate,used
R2716 T3737 T3738 amod total,RNA
R2717 T3738 T3736 dobj RNA,isolate
R2718 T3739 T3736 prep from,isolate
R2719 T3740 T3739 pobj cells,from
R2720 T3741 T3734 punct .,used
R2721 T3742 T3751 prep For,was
R2722 T3743 T3744 compound cDNA,synthesis
R2723 T3744 T3742 pobj synthesis,For
R2724 T3745 T3751 punct ",",was
R2725 T3746 T3747 nummod 1,µg
R2726 T3747 T3751 nsubj µg,was
R2727 T3748 T3747 prep of,µg
R2728 T3749 T3750 amod total,RNA
R2729 T3750 T3748 pobj RNA,of
R2730 T3751 T3751 ROOT was,was
R2731 T3752 T3753 advmod reverse,transcribed
R2732 T3753 T3751 conj transcribed,was
R2733 T3754 T3753 advcl using,transcribed
R2734 T3755 T3756 compound TaqMan,®
R2735 T3756 T3757 nsubj ®,Reverse
R2736 T3757 T3754 xcomp Reverse,using
R2737 T3758 T3759 compound Transcription,Reagents
R2738 T3759 T3757 dobj Reagents,Reverse
R2739 T3760 T3762 punct (,Biosystems
R2740 T3761 T3762 compound Applied,Biosystems
R2741 T3762 T3759 appos Biosystems,Reagents
R2742 T3763 T3762 punct ),Biosystems
R2743 T3764 T3757 punct .,Reverse
R2744 T3765 T3767 nsubjpass qPCR,performed
R2745 T3766 T3767 auxpass was,performed
R2746 T3767 T3767 ROOT performed,performed
R2747 T3768 T3767 advcl using,performed
R2748 T3769 T3773 compound TaqMan,20X
R2749 T3770 T3773 compound ®,20X
R2750 T3771 T3773 compound Gene,20X
R2751 T3772 T3773 compound Expression,20X
R2752 T3773 T3774 compound 20X,primers
R2753 T3774 T3768 dobj primers,using
R2754 T3775 T3768 prep for,using
R2755 T3776 T3775 pobj Serpinb2/SerpinB2,for
R2756 T3777 T3778 punct (,Mm00440905_m1
R2757 T3778 T3776 appos Mm00440905_m1,Serpinb2/SerpinB2
R2758 T3779 T3776 punct ),Serpinb2/SerpinB2
R2759 T3780 T3776 punct ",",Serpinb2/SerpinB2
R2760 T3781 T3776 conj Cebpb,Serpinb2/SerpinB2
R2761 T3782 T3783 punct (,Mm00843434_s1
R2762 T3783 T3781 appos Mm00843434_s1,Cebpb
R2763 T3784 T3783 punct ),Mm00843434_s1
R2764 T3785 T3781 cc and,Cebpb
R2765 T3786 T3781 conj β-actin,Cebpb
R2766 T3787 T3786 punct (,β-actin
R2767 T3788 T3786 appos Mm00607939_s1,β-actin
R2768 T3789 T3786 punct ),β-actin
R2769 T3790 T3792 punct (,Biosystems
R2770 T3791 T3792 compound Applied,Biosystems
R2771 T3792 T3786 appos Biosystems,β-actin
R2772 T3793 T3767 punct ),performed
R2773 T3794 T3767 punct .,performed
R2952 T4058 T4060 compound DNA,Analysis
R2953 T4059 T4060 compound Sequence,Analysis
R2954 T4060 T4070 nsubjpass Analysis,determined
R2955 T4061 T4063 det The,sequence
R2956 T4062 T4063 compound DNA,sequence
R2957 T4063 T4060 appos sequence,Analysis
R2958 T4064 T4063 prep of,sequence
R2959 T4065 T4068 det the,promoter
R2960 T4066 T4068 compound murine,promoter
R2961 T4067 T4068 compound SerpinB2,promoter
R2962 T4068 T4064 pobj promoter,of
R2963 T4069 T4070 auxpass was,determined
R2964 T4070 T4070 ROOT determined,determined
R2965 T4071 T4070 prep by,determined
R2966 T4072 T4071 pcomp sequencing,by
R2967 T4073 T4075 det the,plasmids
R2968 T4074 T4075 amod pUC-based,plasmids
R2969 T4075 T4072 dobj plasmids,sequencing
R2970 T4076 T4072 npadvmod pDB9406,sequencing
R2971 T4077 T4076 punct ",",pDB9406
R2972 T4078 T4076 conj pDB9402-41,pDB9406
R2973 T4079 T4078 cc and,pDB9402-41
R2974 T4080 T4078 conj pDB9402-42,pDB9402-41
R2975 T4081 T4072 punct ",",sequencing
R2976 T4082 T4072 prep in,sequencing
R2977 T4083 T4082 pobj addition,in
R2978 T4084 T4083 prep to,addition
R2979 T4085 T4084 pobj plasmids,to
R2980 T4086 T4085 acl prepared,plasmids
R2981 T4087 T4086 prep from,prepared
R2982 T4088 T4087 pobj pDB9402-41,from
R2983 T4089 T4088 cc and,pDB9402-41
R2984 T4090 T4088 conj pDB9402-42,pDB9402-41
R2985 T4091 T4085 prep containing,plasmids
R2986 T4092 T4091 dobj deletions,containing
R2987 T4093 T4092 acl introduced,deletions
R2988 T4094 T4093 agent by,introduced
R2989 T4095 T4097 amod exonuclease,digestion
R2990 T4096 T4097 compound III,digestion
R2991 T4097 T4094 pobj digestion,by
R2992 T4098 T4070 punct .,determined
R2993 T4099 T4100 compound Plasmids,pDB9406
R2994 T4100 T4100 ROOT pDB9406,pDB9406
R2995 T4101 T4100 punct ",",pDB9406
R2996 T4102 T4100 conj pDB9402-41,pDB9406
R2997 T4103 T4102 cc and,pDB9402-41
R2998 T4104 T4102 conj pDB9402-42,pDB9402-41
R2999 T4105 T4108 prep containing,isolated
R3000 T4106 T4107 compound genomic,DNA
R3001 T4107 T4108 nsubj DNA,isolated
R3002 T4108 T4108 ROOT isolated,isolated
R3003 T4109 T4108 prep from,isolated
R3004 T4110 T4111 det a,λFIXII
R3005 T4111 T4116 nmod λFIXII,library
R3006 T4112 T4111 punct (,λFIXII
R3007 T4113 T4111 appos Stratagene,λFIXII
R3008 T4114 T4111 punct ),λFIXII
R3009 T4115 T4116 amod genomic,library
R3010 T4116 T4109 pobj library,from
R3011 T4117 T4108 advcl prepared,isolated
R3012 T4118 T4117 prep from,prepared
R3013 T4119 T4122 det a,strain
R3014 T4120 T4122 nummod 129,strain
R3015 T4121 T4122 compound mouse,strain
R3016 T4122 T4118 pobj strain,from
R3017 T4123 T4108 punct ",",isolated
R3018 T4124 T4126 auxpass were,provided
R3019 T4125 T4126 advmod kindly,provided
R3020 T4126 T4108 advcl provided,isolated
R3021 T4127 T4126 agent by,provided
R3022 T4128 T4130 compound Dr.,Belin
R3023 T4129 T4130 compound Dominique,Belin
R3024 T4130 T4127 pobj Belin,by
R3025 T4131 T4130 punct ",",Belin
R3026 T4132 T4130 conj University,Belin
R3027 T4133 T4132 prep of,University
R3028 T4134 T4133 pobj Geneva,of
R3029 T4135 T4108 punct .,isolated
R3030 T4136 T4137 nsubj pDB9406,contains
R3031 T4137 T4153 ccomp contains,contain
R3032 T4138 T4143 det a,fragment
R3033 T4139 T4140 nummod 4.4,kb
R3034 T4140 T4143 nmod kb,fragment
R3035 T4141 T4143 nmod EcoRI/SpeI,fragment
R3036 T4142 T4143 compound genomic,fragment
R3037 T4143 T4137 dobj fragment,contains
R3038 T4144 T4143 acl spanning,fragment
R3039 T4145 T4148 det the,site
R3040 T4146 T4147 compound transcription,initiation
R3041 T4147 T4148 compound initiation,site
R3042 T4148 T4144 dobj site,spanning
R3043 T4149 T4153 punct ;,contain
R3044 T4150 T4153 nsubj pDB9402-41,contain
R3045 T4151 T4150 cc and,pDB9402-41
R3046 T4152 T4150 conj pDB9402-42,pDB9402-41
R3047 T4153 T4153 ROOT contain,contain
R3048 T4154 T4159 det a,fragment
R3049 T4155 T4156 nummod 1.2,kb
R3050 T4156 T4159 nmod kb,fragment
R3051 T4157 T4159 nmod EcoRI,fragment
R3052 T4158 T4159 compound genomic,fragment
R3053 T4159 T4153 dobj fragment,contain
R3054 T4160 T4159 punct ",",fragment
R3055 T4161 T4159 acl located,fragment
R3056 T4162 T4163 advmod immediately,upstream
R3057 T4163 T4161 advmod upstream,located
R3058 T4164 T4163 prep of,upstream
R3059 T4165 T4168 det the,fragment
R3060 T4166 T4167 compound pDB9406,EcoRI
R3061 T4167 T4168 compound EcoRI,fragment
R3062 T4168 T4164 pobj fragment,of
R3063 T4169 T4161 punct ",",located
R3064 T4170 T4159 acl cloned,fragment
R3065 T4171 T4170 prep in,cloned
R3066 T4172 T4173 amod opposite,orientations
R3067 T4173 T4171 pobj orientations,in
R3068 T4174 T4153 punct .,contain
R3069 T4175 T4176 amod Subcloned,inserts
R3070 T4176 T4178 nsubjpass inserts,verified
R3071 T4177 T4178 auxpass were,verified
R3072 T4178 T4178 ROOT verified,verified
R3073 T4179 T4178 agent by,verified
R3074 T4180 T4181 compound restriction,enzyme
R3075 T4181 T4182 compound enzyme,digestion
R3076 T4182 T4179 pobj digestion,by
R3077 T4183 T4182 cc and,digestion
R3078 T4184 T4185 compound DNA,sequencing
R3079 T4185 T4182 conj sequencing,digestion
R3080 T4186 T4178 punct .,verified
R3081 T4187 T4189 det The,sequence
R3082 T4188 T4189 compound nucleotide,sequence
R3083 T4189 T4202 nsubjpass sequence,determined
R3084 T4190 T4189 prep of,sequence
R3085 T4191 T4196 det the,gene
R3086 T4192 T4196 nummod 4480,gene
R3087 T4193 T4196 nmod bp,gene
R3088 T4194 T4196 compound murine,gene
R3089 T4195 T4196 compound SerpinB2,gene
R3090 T4196 T4200 nmod gene,region
R3091 T4197 T4198 nummod 5,′
R3092 T4198 T4200 amod ′,region
R3093 T4199 T4200 amod flanking,region
R3094 T4200 T4190 pobj region,of
R3095 T4201 T4202 auxpass was,determined
R3096 T4202 T4202 ROOT determined,determined
R3097 T4203 T4202 advcl using,determined
R3098 T4204 T4209 det the,cycle
R3099 T4205 T4209 compound ABI,cycle
R3100 T4206 T4209 compound PRISM,cycle
R3101 T4207 T4208 compound dye,terminator
R3102 T4208 T4209 compound terminator,cycle
R3103 T4209 T4203 dobj cycle,using
R3104 T4210 T4203 conj sequencing,using
R3105 T4211 T4213 amod ready,kit
R3106 T4212 T4213 compound reaction,kit
R3107 T4213 T4210 dobj kit,sequencing
R3108 T4214 T4213 punct (,kit
R3109 T4215 T4210 conj Perkin-Elmer,sequencing
R3110 T4216 T4215 punct ),Perkin-Elmer
R3111 T4217 T4210 cc and,sequencing
R3112 T4218 T4221 det a,sequencer
R3113 T4219 T4221 compound PE,sequencer
R3114 T4220 T4221 compound 373A,sequencer
R3115 T4221 T4210 conj sequencer,sequencing
R3116 T4222 T4221 punct (,sequencer
R3117 T4223 T4221 appos Perkin-Elmer,sequencer
R3118 T4224 T4221 punct ),sequencer
R3119 T4225 T4202 punct .,determined
R3120 T4226 T4227 det This,sequence
R3121 T4227 T4229 nsubjpass sequence,deposited
R3122 T4228 T4229 auxpass was,deposited
R3123 T4229 T4229 ROOT deposited,deposited
R3124 T4230 T4229 prep in,deposited
R3125 T4231 T4233 compound GenBank/EMBL/DDBJ,Bank
R3126 T4232 T4233 compound Data,Bank
R3127 T4233 T4230 pobj Bank,in
R3128 T4234 T4229 prep with,deposited
R3129 T4235 T4236 compound Accession,No
R3130 T4236 T4234 pobj No,with
R3131 T4237 T4229 punct .,deposited
R3132 T4238 T4238 ROOT AF339731,AF339731
R3133 T4239 T4238 punct .,AF339731
R3322 T4532 T4528 appos deoxycholate,NP-40
R3323 T4533 T4532 punct ",",deoxycholate
R3324 T4534 T4535 nummod 0.1,%
R3325 T4535 T4536 compound %,SDS
R3326 T4536 T4532 appos SDS,deoxycholate
R3327 T4537 T4536 punct ),SDS
R3328 T4538 T4541 punct ",",separated
R3329 T4539 T4541 nsubjpass proteins,separated
R3330 T4540 T4541 auxpass were,separated
R3331 T4541 T4541 ROOT separated,separated
R3332 T4542 T4541 prep on,separated
R3333 T4543 T4542 pobj 4,on
R3334 T4544 T4545 nummod 12,%
R3335 T4545 T4548 compound %,gels
R3336 T4546 T4547 compound Bis-Tris,NuPAGE
R3337 T4547 T4548 compound NuPAGE,gels
R3338 T4548 T4541 appos gels,separated
R3339 T4549 T4550 punct (,Invitrogen
R3340 T4550 T4548 appos Invitrogen,gels
R3341 T4551 T4548 punct ),gels
R3342 T4552 T4541 punct ",",separated
R3291 T4501 T4506 amod Western,lysates
R3292 T4502 T4506 compound Blot,lysates
R3293 T4503 T4506 compound Assays,lysates
R3294 T4504 T4506 compound Whole,lysates
R3295 T4505 T4506 compound cell,lysates
R3296 T4506 T4508 nsubjpass lysates,prepared
R3297 T4507 T4508 auxpass were,prepared
R3298 T4508 T4541 ccomp prepared,separated
R3299 T4509 T4508 prep in,prepared
R3300 T4510 T4511 compound RIPA,buffer
R3301 T4511 T4509 pobj buffer,in
R3302 T4512 T4515 punct (,Tris
R3303 T4513 T4515 nummod 10,Tris
R3304 T4514 T4515 compound mM,Tris
R3305 T4515 T4541 nsubjpass Tris,separated
R3306 T4516 T4515 punct ",",Tris
R3307 T4517 T4519 nummod 150,NaCl
R3308 T4518 T4519 compound mM,NaCl
R3309 T4519 T4515 appos NaCl,Tris
R3310 T4520 T4519 punct ",",NaCl
R3311 T4521 T4522 nummod 1,%
R3312 T4522 T4524 compound %,X-100
R3313 T4523 T4524 compound Triton,X-100
R3314 T4524 T4519 conj X-100,NaCl
R3315 T4525 T4524 punct ",",X-100
R3316 T4526 T4527 nummod 0.5,%
R3317 T4527 T4528 compound %,NP-40
R3318 T4528 T4524 appos NP-40,X-100
R3319 T4529 T4528 punct ",",NP-40
R3320 T4530 T4531 nummod 0.5,%
R3321 T4531 T4532 compound %,deoxycholate

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4344 2572-2573 -COMMA- denotes ,
T4343 2571-2572 -RRB- denotes )
T4342 2561-2571 NN denotes Invitrogen
T4341 2560-2561 -LRB- denotes (
T4340 2555-2559 NN denotes gels
T4339 2548-2554 NN denotes NuPAGE
T4338 2539-2547 JJ denotes Bis-Tris
T4337 2537-2538 NN denotes %
T4336 2533-2537 CD denotes 4–12
T4310 2435-2438 CD denotes 150
T4309 2433-2434 -COMMA- denotes ,
T4308 2429-2433 NNP denotes Tris
T4307 2426-2428 NN denotes mM
T4306 2423-2425 CD denotes 10
T4305 2422-2423 -LRB- denotes (
T4304 2415-2421 NN denotes buffer
T4303 2410-2414 NN denotes RIPA
T4302 2407-2409 IN denotes in
T4301 2398-2406 VB denotes prepared
T4300 2393-2397 VB denotes were
T4299 2385-2392 NN denotes lysates
T4298 2380-2384 NN denotes cell
T4297 2374-2379 JJ denotes Whole
T4296 2367-2373 NN denotes Assays
T4295 2362-2366 NNP denotes Blot
T4294 2354-2361 NNP denotes Western
T4008 2343-2351 NN denotes AF339731
T4007 2339-2342 NNP denotes No.
T4006 2329-2338 NNP denotes Accession
T4005 2324-2328 IN denotes with
T4004 2319-2323 NNP denotes Bank
T4003 2314-2318 NNP denotes Data
T4002 2296-2313 NNP denotes GenBank/EMBL/DDBJ
T4001 2293-2295 IN denotes in
T4000 2283-2292 VB denotes deposited
T3999 2279-2282 VB denotes was
T3998 2270-2278 NN denotes sequence
T3997 2265-2269 DT denotes This
T3996 2262-2263 -RRB- denotes )
T3995 2250-2262 NN denotes Perkin-Elmer
T3994 2249-2250 -LRB- denotes (
T3993 2239-2248 NN denotes sequencer
T3992 2234-2238 NN denotes 373A
T3991 2231-2233 NN denotes PE
T3990 2229-2230 DT denotes a
T3989 2225-2228 CC denotes and
T3988 2223-2224 -RRB- denotes )
T3987 2211-2223 NN denotes Perkin-Elmer
T3986 2210-2211 -LRB- denotes (
T3985 2206-2209 NN denotes kit
T3984 2197-2205 NN denotes reaction
T3983 2191-2196 JJ denotes ready
T3982 2180-2190 NN denotes sequencing
T3981 2174-2179 NN denotes cycle
T3980 2163-2173 NN denotes terminator
T3979 2159-2162 NN denotes dye
T3978 2153-2158 NNP denotes PRISM
T3977 2149-2152 NNP denotes ABI
T3976 2145-2148 DT denotes the
T3975 2139-2144 VB denotes using
T3974 2128-2138 VB denotes determined
T3973 2124-2127 VB denotes was
T3972 2117-2123 NN denotes region
T3971 2108-2116 NN denotes flanking
T3970 2105-2107 NN denotes 5′
T3969 2100-2104 NN denotes gene
T3968 2091-2099 NN denotes SerpinB2
T3967 2084-2090 JJ denotes murine
T3966 2081-2083 NN denotes bp
T3965 2076-2080 CD denotes 4480
T3964 2072-2075 DT denotes the
T3963 2069-2071 IN denotes of
T3962 2060-2068 NN denotes sequence
T3961 2049-2059 NN denotes nucleotide
T3960 2045-2048 DT denotes The
T3959 2033-2043 NN denotes sequencing
T3958 2029-2032 NN denotes DNA
T3957 2025-2028 CC denotes and
T3956 2015-2024 NN denotes digestion
T3955 2008-2014 NN denotes enzyme
T3954 1996-2007 NN denotes restriction
T3953 1993-1995 IN denotes by
T3952 1984-1992 VB denotes verified
T3951 1979-1983 VB denotes were
T3950 1971-1978 NN denotes inserts
T3949 1961-1970 VB denotes Subcloned
T3948 1947-1959 NN denotes orientations
T3947 1938-1946 JJ denotes opposite
T3946 1935-1937 IN denotes in
T3945 1928-1934 VB denotes cloned
T3944 1926-1927 -COMMA- denotes ,
T3943 1918-1926 NN denotes fragment
T3942 1912-1917 NN denotes EcoRI
T3941 1904-1911 NN denotes pDB9406
T3940 1900-1903 DT denotes the
T3939 1897-1899 IN denotes of
T3938 1888-1896 RB denotes upstream
T3937 1876-1887 RB denotes immediately
T3936 1868-1875 JJ denotes located
T3935 1866-1867 -COMMA- denotes ,
T3934 1858-1866 NN denotes fragment
T3933 1850-1857 JJ denotes genomic
T3932 1844-1849 NN denotes EcoRI
T3931 1841-1843 NN denotes kb
T3930 1837-1840 CD denotes 1.2
T3929 1835-1836 DT denotes a
T3928 1827-1834 VB denotes contain
T3927 1816-1826 NN denotes pDB9402-42
T3926 1812-1815 CC denotes and
T3925 1801-1811 NN denotes pDB9402-41
T3924 1799-1800 -COLON- denotes ;
T3923 1795-1799 NN denotes site
T3922 1784-1794 NN denotes initiation
T3921 1770-1783 NN denotes transcription
T3920 1766-1769 DT denotes the
T3919 1757-1765 VB denotes spanning
T3918 1748-1756 NN denotes fragment
T3917 1740-1747 JJ denotes genomic
T3916 1729-1739 NN denotes EcoRI/SpeI
T3915 1726-1728 NN denotes kb
T3914 1722-1725 CD denotes 4.4
T3913 1720-1721 DT denotes a
T3912 1711-1719 VB denotes contains
T3911 1703-1710 NN denotes pDB9406
T3910 1695-1702 NNP denotes Geneva.
T3909 1692-1694 IN denotes of
T3908 1681-1691 NNP denotes University
T3907 1679-1680 -COMMA- denotes ,
T3906 1674-1679 NNP denotes Belin
T3905 1664-1673 NNP denotes Dominique
T3904 1660-1663 NNP denotes Dr.
T3903 1657-1659 IN denotes by
T3902 1648-1656 VB denotes provided
T3901 1641-1647 RB denotes kindly
T3900 1636-1640 VB denotes were
T3899 1634-1635 -COMMA- denotes ,
T3898 1628-1634 NN denotes strain
T3897 1622-1627 NN denotes mouse
T3896 1618-1621 CD denotes 129
T3895 1616-1617 DT denotes a
T3894 1611-1615 IN denotes from
T3893 1602-1610 VB denotes prepared
T3892 1594-1601 NN denotes library
T3891 1586-1593 JJ denotes genomic
T3890 1584-1585 -RRB- denotes )
T3889 1574-1584 NN denotes Stratagene
T3888 1573-1574 -LRB- denotes (
T3887 1566-1572 NN denotes λFIXII
T3886 1564-1565 DT denotes a
T3885 1559-1563 IN denotes from
T3884 1550-1558 VB denotes isolated
T3883 1546-1549 NN denotes DNA
T3882 1538-1545 JJ denotes genomic
T3881 1527-1537 VB denotes containing
T3880 1516-1526 NN denotes pDB9402-42
T3879 1512-1515 CC denotes and
T3878 1501-1511 NN denotes pDB9402-41
T3877 1499-1500 -COMMA- denotes ,
T3876 1492-1499 NN denotes pDB9406
T3875 1483-1491 NN denotes Plasmids
T3874 1472-1481 NN denotes digestion
T4335 2530-2532 IN denotes on
T4334 2520-2529 VB denotes separated
T4333 2515-2519 VB denotes were
T4332 2506-2514 NN denotes proteins
T4331 2504-2505 -COMMA- denotes ,
T4330 2503-2504 -RRB- denotes )
T4329 2500-2503 NN denotes SDS
T4328 2498-2499 NN denotes %
T4327 2495-2498 CD denotes 0.1
T4326 2493-2494 -COMMA- denotes ,
T4325 2481-2493 NN denotes deoxycholate
T4324 2479-2480 NN denotes %
T4323 2476-2479 CD denotes 0.5
T4322 2474-2475 -COMMA- denotes ,
T4321 2469-2474 NN denotes NP-40
T4320 2467-2468 NN denotes %
T4319 2464-2467 CD denotes 0.5
T4318 2462-2463 -COMMA- denotes ,
T4317 2457-2462 NN denotes X-100
T4316 2450-2456 NN denotes Triton
T4315 2449-2450 NN denotes %
T4314 2448-2449 CD denotes 1
T4313 2446-2447 -COMMA- denotes ,
T4312 2442-2446 NN denotes NaCl
T4311 2439-2441 NN denotes mM
T3873 1468-1471 CD denotes III
T3872 1456-1467 NN denotes exonuclease
T3871 1453-1455 IN denotes by
T3870 1442-1452 VB denotes introduced
T3869 1432-1441 NN denotes deletions
T3868 1421-1431 VB denotes containing
T3867 1410-1420 NN denotes pDB9402-42
T3866 1406-1409 CC denotes and
T3865 1395-1405 NN denotes pDB9402-41
T3864 1390-1394 IN denotes from
T3863 1381-1389 VB denotes prepared
T3862 1372-1380 NN denotes plasmids
T3861 1369-1371 TO denotes to
T3860 1360-1368 NN denotes addition
T3859 1357-1359 IN denotes in
T3858 1355-1356 -COMMA- denotes ,
T3857 1345-1355 NN denotes pDB9402-42
T3856 1341-1344 CC denotes and
T3855 1330-1340 NN denotes pDB9402-41
T3854 1328-1329 -COMMA- denotes ,
T3853 1321-1328 NN denotes pDB9406
T3852 1312-1320 NN denotes plasmids
T3851 1302-1311 JJ denotes pUC-based
T3850 1298-1301 DT denotes the
T3849 1287-1297 VB denotes sequencing
T3848 1284-1286 IN denotes by
T3673 1176-1177 -RRB- denotes A
T3672 1166-1176 NNP denotes 7939_s1) (
T3671 1159-1166 NNP denotes (Mm0060
T3670 1157-1158 -LRB- denotes n
T3669 1156-1157 -RRB- denotes i
T3668 1143-1156 NN denotes s1) and β-act
T3667 1142-1143 -LRB- denotes _
T3666 1135-1142 NN denotes 0843434
T3665 1132-1135 CC denotes Mm0
T3664 1131-1132 -RRB- denotes (
T3663 1117-1130 NN denotes 05_m1), Cebpb
T3662 1116-1117 -LRB- denotes 9
T3661 1111-1116 NN denotes 00440
T3660 1110-1111 -COMMA- denotes m
T3659 1109-1110 -RRB- denotes M
T3658 1096-1109 NN denotes b2/SerpinB2 (
T3657 1095-1096 -LRB- denotes n
T3656 1078-1095 NN denotes primers for Serpi
T3655 1075-1078 IN denotes 0X
T3654 1068-1075 NN denotes ssion 2
T3653 1065-1068 NN denotes pre
T3652 1055-1065 NN denotes n® Gene Ex
T3651 1051-1055 NNP denotes aqMa
T3650 1044-1051 NNP denotes using T
T3649 1039-1044 VB denotes rmed
T3648 1030-1039 VB denotes was perfo
T3647 1027-1030 VB denotes CR
T3646 1023-1027 NN denotes . qP
T3645 1022-1023 -RRB- denotes )
T3644 1012-1022 NNP denotes Biosystems
T3643 1004-1011 NNP denotes Applied
T3642 1003-1004 -LRB- denotes (
T3641 994-1002 NN denotes Reagents
T3640 980-993 NN denotes Transcription
T3639 972-979 NNP denotes Reverse
T3638 964-971 NNP denotes TaqMan®
T3637 958-963 VB denotes using
T3636 946-957 VB denotes transcribed
T3635 938-945 RB denotes reverse
T3634 934-937 VB denotes was
T3633 930-933 NN denotes RNA
T3632 924-929 JJ denotes total
T3631 921-923 IN denotes of
T3630 918-920 NN denotes µg
T3629 916-917 CD denotes 1
T3628 914-915 -COMMA- denotes ,
T3627 905-914 NN denotes synthesis
T3626 900-904 NN denotes cDNA
T3625 896-899 IN denotes For
T3624 889-894 NN denotes cells
T3623 884-888 IN denotes from
T3622 880-883 NN denotes RNA
T3621 874-879 JJ denotes total
T3620 866-873 VB denotes isolate
T3619 863-865 TO denotes to
T3618 858-862 VB denotes used
T3617 854-857 VB denotes was
T3616 852-853 -RRB- denotes )
T3615 846-852 NN denotes Qiagen
T3614 845-846 -LRB- denotes (
T3613 841-844 NNP denotes Kit
T3612 836-840 NNP denotes Mini
T3611 829-835 NNP denotes RNeasy
T3610 825-828 DT denotes The
T3609 823-824 -RRB- denotes )
T3608 819-823 NN denotes qPCR
T3607 818-819 -LRB- denotes (
T3606 814-817 NN denotes PCR
T3605 801-813 JJ denotes Quantitative
T3287 783-788 NNP denotes Sigma
T3286 778-782 IN denotes from
T3285 769-777 VB denotes obtained
T3284 765-768 VB denotes was
T3283 763-764 -RRB- denotes )
T3282 758-763 NN denotes Re595
T3281 751-757 NN denotes Strain
T3280 741-750 NN denotes minnesota
T3279 730-740 NN denotes Salmonella
T3278 729-730 -LRB- denotes (
T3277 727-728 -RRB- denotes )
T3276 724-727 NN denotes LPS
T3275 723-724 -LRB- denotes (
T3274 704-722 NN denotes lipopolysaccharide
T3273 694-703 JJ denotes Bacterial
T3272 691-692 -RRB- denotes )
T3271 686-691 NN denotes Lonza
T3270 685-686 -LRB- denotes (
T3269 681-684 NN denotes Kit
T3268 671-680 NNP denotes Detection
T3267 661-670 NNP denotes MycoAlert
T3266 657-660 DT denotes the
T3265 653-656 CC denotes and
T3264 651-652 -RRB- denotes )
T3263 646-651 NN denotes Sigma
T3262 645-646 -LRB- denotes (
T3261 639-644 CD denotes 33258
T3260 633-638 NN denotes stain
T3259 625-632 NNP denotes Hoechst
T3258 619-624 VB denotes using
T3257 610-618 NN denotes staining
T3256 602-609 JJ denotes nuclear
T3255 599-601 IN denotes by
T3254 589-598 NN denotes infection
T3253 578-588 NN denotes Mycoplasma
T3252 570-577 VB denotes exclude
T3251 567-569 TO denotes to
T3250 559-566 VB denotes checked
T3249 549-558 RB denotes routinely
T3248 544-548 VB denotes were
T3247 535-543 NN denotes cultures
T3246 531-534 DT denotes All
T3245 523-529 NN denotes method
T3244 513-522 NN denotes exclusion
T3243 509-512 NN denotes dye
T3242 507-508 -RRB- denotes )
T3241 502-507 NN denotes Sigma
T3240 501-502 -LRB- denotes (
T3239 496-500 JJ denotes blue
T3238 489-495 NN denotes trypan
T3237 485-488 DT denotes the
T3236 479-484 VB denotes using
T3235 468-478 VB denotes determined
T3234 464-467 VB denotes was
T3233 454-463 NN denotes viability
T3232 449-453 NN denotes Cell
T3231 442-447 NN denotes serum
T3230 433-441 JJ denotes LPS-free
T3229 423-432 VB denotes certified
T3228 420-422 IN denotes of
T3227 416-419 NN denotes use
T3226 412-415 DT denotes the
T3225 404-411 IN denotes through
T3224 395-403 NN denotes cultures
T3223 390-394 NN denotes cell
T3222 386-389 DT denotes all
T3221 381-385 IN denotes from
T3220 367-380 NN denotes contamination
T3219 348-366 NN denotes lipopolysaccharide
T3218 338-347 JJ denotes bacterial
T3217 330-337 VB denotes exclude
T3216 327-329 TO denotes to
T3215 321-326 VB denotes taken
T3214 316-320 VB denotes were
T3213 304-315 NN denotes Precautions
T3212 301-302 -RRB- denotes )
T3211 298-301 NNP denotes BRL
T3210 292-297 NNP denotes Gibco
T3209 291-292 -LRB- denotes (
T3208 285-290 NN denotes media
T3207 276-284 NN denotes DMEM/F12
T3206 274-275 NN denotes %
T3205 272-274 CD denotes 50
T3204 269-271 IN denotes in
T3203 258-268 VB denotes maintained
T3202 254-257 CC denotes and
T3201 252-253 -COMMA- denotes ,
T3200 247-252 RB denotes later
T3199 242-246 NN denotes days
T3198 238-241 CD denotes 3–5
T3197 231-237 NN denotes lavage
T3196 220-230 JJ denotes peritoneal
T3195 217-219 IN denotes by
T3194 208-216 VB denotes followed
T3193 202-207 NN denotes broth
T3192 187-201 NN denotes thioglycollate
T3191 185-186 NN denotes %
T3190 184-185 CD denotes 3
T3189 179-183 IN denotes with
T3188 174-178 NN denotes mice
T3187 166-173 NN denotes C57BL/6
T3186 156-165 VB denotes injecting
T3185 153-155 IN denotes by
T3184 144-152 VB denotes obtained
T3183 139-143 VB denotes were
T3182 127-138 NN denotes macrophages
T3181 116-126 JJ denotes peritoneal
T3180 108-115 JJ denotes Primary
T3847 1273-1283 VB denotes determined
T3846 1269-1272 VB denotes was
T3845 1260-1268 NN denotes promoter
T3844 1251-1259 NN denotes SerpinB2
T3843 1244-1250 JJ denotes murine
T3842 1240-1243 DT denotes the
T3841 1237-1239 IN denotes of
T3840 1228-1236 NN denotes sequence
T3839 1224-1227 NN denotes DNA
T3838 1220-1223 DT denotes The
T3837 1211-1219 NN denotes Analysis
T3836 1202-1210 NN denotes Sequence
T3835 1198-1201 NN denotes DNA
T3604 791-800 JJ denotes Real-time
R2266 T3182 T3180 arg1Of macrophages,Primary
R2267 T3182 T3181 arg1Of macrophages,peritoneal
R2268 T3182 T3183 arg1Of macrophages,were
R2269 T3182 T3184 arg2Of macrophages,obtained
R2270 T3182 T3203 arg1Of macrophages,maintained
R2271 T3184 T3183 arg2Of obtained,were
R2272 T3184 T3185 arg1Of obtained,by
R2273 T3184 T3202 arg1Of obtained,and
R2274 T3186 T3185 arg2Of injecting,by
R2275 T3188 T3186 arg2Of mice,injecting
R2276 T3188 T3187 arg1Of mice,C57BL/6
R2277 T3188 T3189 arg1Of mice,with
R2278 T3190 T3191 arg1Of 3,%
R2279 T3193 T3189 arg2Of broth,with
R2280 T3193 T3190 arg1Of broth,3
R2281 T3193 T3192 arg1Of broth,thioglycollate
R2282 T3193 T3194 arg2Of broth,followed
R2283 T3194 T3200 arg1Of followed,later
R2284 T3197 T3194 arg1Of lavage,followed
R2285 T3197 T3195 arg2Of lavage,by
R2286 T3197 T3196 arg1Of lavage,peritoneal
R2287 T3199 T3198 arg1Of days,3–5
R2288 T3200 T3199 arg1Of later,days
R2289 T3202 T3201 arg1Of and,","
R2290 T3203 T3202 arg2Of maintained,and
R2291 T3203 T3204 arg1Of maintained,in
R2292 T3205 T3206 arg1Of 50,%
R2293 T3208 T3204 arg2Of media,in
R2294 T3208 T3205 arg1Of media,50
R2295 T3208 T3207 arg1Of media,DMEM/F12
R2296 T3208 T3209 arg1Of media,(
R2297 T3211 T3209 arg2Of BRL,(
R2298 T3211 T3210 arg1Of BRL,Gibco
R2299 T3212 T3209 arg3Of ),(
R2300 T3213 T3214 arg1Of Precautions,were
R2301 T3213 T3215 arg2Of Precautions,taken
R2302 T3213 T3217 arg1Of Precautions,exclude
R2303 T3215 T3214 arg2Of taken,were
R2304 T3217 T3215 arg3Of exclude,taken
R2305 T3217 T3216 arg1Of exclude,to
R2306 T3217 T3225 arg1Of exclude,through
R2307 T3220 T3217 arg2Of contamination,exclude
R2308 T3220 T3218 arg1Of contamination,bacterial
R2309 T3220 T3219 arg1Of contamination,lipopolysaccharide
R2310 T3220 T3221 arg1Of contamination,from
R2311 T3224 T3221 arg2Of cultures,from
R2312 T3224 T3222 arg1Of cultures,all
R2313 T3224 T3223 arg1Of cultures,cell
R2314 T3227 T3225 arg2Of use,through
R2315 T3227 T3226 arg1Of use,the
R2316 T3227 T3228 arg1Of use,of
R2317 T3231 T3228 arg2Of serum,of
R2318 T3231 T3229 arg2Of serum,certified
R2319 T3231 T3230 arg1Of serum,LPS-free
R2320 T3233 T3232 arg1Of viability,Cell
R2321 T3233 T3234 arg1Of viability,was
R2322 T3233 T3235 arg2Of viability,determined
R2323 T3235 T3234 arg2Of determined,was
R2324 T3236 T3235 arg3Of using,determined
R2325 T3241 T3240 arg2Of Sigma,(
R2326 T3242 T3240 arg3Of ),(
R2327 T3245 T3236 arg2Of method,using
R2328 T3245 T3237 arg1Of method,the
R2329 T3245 T3238 arg1Of method,trypan
R2330 T3245 T3239 arg1Of method,blue
R2331 T3245 T3240 arg1Of method,(
R2332 T3245 T3243 arg1Of method,dye
R2333 T3245 T3244 arg1Of method,exclusion
R2334 T3247 T3246 arg1Of cultures,All
R2335 T3247 T3248 arg1Of cultures,were
R2336 T3247 T3250 arg2Of cultures,checked
R2337 T3250 T3248 arg2Of checked,were
R2338 T3250 T3249 arg1Of checked,routinely
R2339 T3250 T3251 modOf checked,to
R2340 T3252 T3251 arg1Of exclude,to
R2341 T3254 T3252 arg2Of infection,exclude
R2342 T3254 T3253 arg1Of infection,Mycoplasma
R2343 T3254 T3255 arg1Of infection,by
R2344 T3257 T3256 arg1Of staining,nuclear
R2345 T3257 T3258 arg1Of staining,using
R2346 T3257 T3265 arg1Of staining,and
R2347 T3260 T3258 arg2Of stain,using
R2348 T3260 T3259 arg1Of stain,Hoechst
R2349 T3260 T3261 arg1Of stain,33258
R2350 T3260 T3262 arg1Of stain,(
R2351 T3263 T3262 arg2Of Sigma,(
R2352 T3264 T3262 arg3Of ),(
R2353 T3265 T3255 arg2Of and,by
R2354 T3268 T3267 arg1Of Detection,MycoAlert
R2355 T3269 T3265 arg2Of Kit,and
R2356 T3269 T3266 arg1Of Kit,the
R2357 T3269 T3268 arg1Of Kit,Detection
R2358 T3269 T3270 arg1Of Kit,(
R2359 T3271 T3270 arg2Of Lonza,(
R2360 T3272 T3270 arg3Of ),(
R2361 T3274 T3273 arg1Of lipopolysaccharide,Bacterial
R2362 T3274 T3275 arg1Of lipopolysaccharide,(
R2363 T3274 T3278 arg1Of lipopolysaccharide,(
R2364 T3274 T3284 arg1Of lipopolysaccharide,was
R2365 T3274 T3285 arg2Of lipopolysaccharide,obtained
R2366 T3276 T3275 arg2Of LPS,(
R2367 T3277 T3275 arg3Of ),(
R2368 T3282 T3278 arg2Of Re595,(
R2369 T3282 T3279 arg1Of Re595,Salmonella
R2370 T3282 T3280 arg1Of Re595,minnesota
R2371 T3282 T3281 arg1Of Re595,Strain
R2372 T3283 T3278 arg3Of ),(
R2373 T3285 T3284 arg2Of obtained,was
R2374 T3285 T3286 arg1Of obtained,from
R2375 T3287 T3286 arg2Of Sigma,from
R2630 T3613 T3614 arg1Of Kit,(
R2632 T3613 T3618 arg2Of Kit,used
R2633 T3613 T3620 arg1Of Kit,isolate
R2634 T3615 T3614 arg2Of Qiagen,(
R2637 T3620 T3618 arg3Of isolate,used
R2638 T3620 T3619 arg1Of isolate,to
R2639 T3620 T3623 arg1Of isolate,from
R2640 T3622 T3620 arg2Of RNA,isolate
R2641 T3622 T3621 arg1Of RNA,total
R2642 T3624 T3623 arg2Of cells,from
R2643 T3627 T3625 arg2Of synthesis,For
R2644 T3627 T3626 arg1Of synthesis,cDNA
R2645 T3630 T3629 arg1Of µg,1
R2646 T3630 T3631 arg1Of µg,of
R2647 T3630 T3634 arg1Of µg,was
R2648 T3630 T3636 arg2Of µg,transcribed
R2649 T3630 T3637 arg1Of µg,using
R2650 T3633 T3631 arg2Of RNA,of
R2651 T3633 T3632 arg1Of RNA,total
R2652 T3636 T3625 arg1Of transcribed,For
R2653 T3636 T3628 arg1Of transcribed,","
R2654 T3636 T3634 arg2Of transcribed,was
R2655 T3636 T3635 arg1Of transcribed,reverse
R2656 T3636 T3637 modOf transcribed,using
R2657 T3639 T3638 arg1Of Reverse,TaqMan®
R2658 T3641 T3637 arg2Of Reagents,using
R2659 T3641 T3639 arg1Of Reagents,Reverse
R2660 T3641 T3640 arg1Of Reagents,Transcription
R2661 T3641 T3642 arg1Of Reagents,(
R2662 T3641 T3647 modOf Reagents,CR
R2663 T3644 T3642 arg2Of Biosystems,(
R2664 T3644 T3643 arg1Of Biosystems,Applied
R2665 T3645 T3642 arg3Of ),(
R2666 T3646 T3647 arg1Of . qP,CR
R2667 T3646 T3648 arg2Of . qP,was perfo
R2668 T3648 T3647 arg2Of was perfo,CR
R2669 T3649 T3648 arg3Of rmed ,was perfo
R2670 T3651 T3650 arg1Of aqMa,using T
R2671 T3654 T3649 arg2Of ssion 2,rmed
R2672 T3654 T3651 arg1Of ssion 2,aqMa
R2673 T3654 T3652 arg1Of ssion 2,n® Gene Ex
R2674 T3654 T3653 arg1Of ssion 2,pre
R2675 T3654 T3655 arg1Of ssion 2,0X
R2676 T3654 T3670 arg1Of ssion 2,n
R2677 T3656 T3657 arg1Of primers for Serpi,n
R2678 T3656 T3660 arg1Of primers for Serpi,m
R2679 T3658 T3657 arg2Of b2/SerpinB2 (,n
R2680 T3659 T3657 arg3Of M,n
R2681 T3660 T3665 arg1Of m,Mm0
R2682 T3661 T3660 arg2Of 00440,m
R2683 T3661 T3662 arg1Of 00440,9
R2684 T3663 T3662 arg2Of "05_m1), Cebpb",9
R2685 T3664 T3662 arg3Of (,9
R2686 T3665 T3655 arg2Of Mm0,0X
R2687 T3666 T3665 arg2Of 0843434,Mm0
R2688 T3666 T3667 arg1Of 0843434,_
R2689 T3668 T3667 arg2Of s1) and β-act,_
R2690 T3669 T3667 arg3Of i,_
R2691 T3672 T3670 arg2Of 7939_s1) (,n
R2692 T3672 T3671 arg1Of 7939_s1) (,(Mm0060
R2693 T3673 T3670 arg3Of A,n
R2778 T3837 T3835 arg1Of Analysis,DNA
R2779 T3837 T3836 arg1Of Analysis,Sequence
R2780 T3840 T3838 arg1Of sequence,The
R2781 T3840 T3839 arg1Of sequence,DNA
R2782 T3840 T3841 arg1Of sequence,of
R2783 T3840 T3846 arg1Of sequence,was
R2784 T3840 T3847 arg2Of sequence,determined
R2785 T3845 T3841 arg2Of promoter,of
R2786 T3845 T3842 arg1Of promoter,the
R2787 T3845 T3843 arg1Of promoter,murine
R2788 T3845 T3844 arg1Of promoter,SerpinB2
R2789 T3847 T3846 arg2Of determined,was
R2790 T3847 T3848 arg1Of determined,by
R2791 T3849 T3848 arg2Of sequencing,by
R2792 T3849 T3858 arg1Of sequencing,","
R2793 T3849 T3859 arg1Of sequencing,in
R2794 T3853 T3854 arg1Of pDB9406,","
R2795 T3854 T3856 arg1Of ",",and
R2796 T3855 T3854 arg2Of pDB9402-41,","
R2797 T3856 T3849 arg2Of and,sequencing
R2798 T3856 T3850 arg1Of and,the
R2799 T3856 T3851 arg1Of and,pUC-based
R2800 T3856 T3852 arg1Of and,plasmids
R2801 T3857 T3856 arg2Of pDB9402-42,and
R2803 T3860 T3861 arg1Of addition,to
R2804 T3862 T3861 arg2Of plasmids,to
R2805 T3862 T3863 arg2Of plasmids,prepared
R2806 T3863 T3864 arg1Of prepared,from
R2807 T3865 T3866 arg1Of pDB9402-41,and
R2808 T3866 T3864 arg2Of and,from
R2809 T3866 T3868 arg1Of and,containing
R2810 T3867 T3866 arg2Of pDB9402-42,and
R2811 T3869 T3868 arg2Of deletions,containing
R2812 T3869 T3870 arg2Of deletions,introduced
R2813 T3874 T3870 arg1Of digestion,introduced
R2814 T3874 T3871 arg2Of digestion,by
R2815 T3874 T3872 arg1Of digestion,exonuclease
R2816 T3874 T3873 arg1Of digestion,III
R2817 T3876 T3877 arg1Of pDB9406,","
R2818 T3877 T3879 arg1Of ",",and
R2819 T3878 T3877 arg2Of pDB9402-41,","
R2820 T3879 T3875 arg1Of and,Plasmids
R2821 T3879 T3881 arg1Of and,containing
R2822 T3879 T3900 arg1Of and,were
R2823 T3879 T3902 arg2Of and,provided
R2824 T3880 T3879 arg2Of pDB9402-42,and
R2825 T3883 T3881 arg2Of DNA,containing
R2826 T3883 T3882 arg1Of DNA,genomic
R2827 T3883 T3884 arg2Of DNA,isolated
R2828 T3884 T3885 arg1Of isolated,from
R2829 T3889 T3888 arg2Of Stratagene,(
R2830 T3890 T3888 arg3Of ),(
R2831 T3892 T3885 arg2Of library,from
R2832 T3892 T3886 arg1Of library,a
R2833 T3892 T3887 arg1Of library,λFIXII
R2834 T3892 T3888 arg1Of library,(
R2835 T3892 T3891 arg1Of library,genomic
R2836 T3892 T3893 arg2Of library,prepared
R2837 T3893 T3894 arg1Of prepared,from
R2838 T3898 T3894 arg2Of strain,from
R2839 T3898 T3895 arg1Of strain,a
R2840 T3898 T3896 arg1Of strain,129
R2841 T3898 T3897 arg1Of strain,mouse
R2842 T3902 T3899 arg1Of provided,","
R2843 T3902 T3900 arg2Of provided,were
R2844 T3902 T3901 arg1Of provided,kindly
R2845 T3902 T3907 arg1Of provided,","
R2846 T3906 T3902 arg1Of Belin,provided
R2847 T3906 T3903 arg2Of Belin,by
R2848 T3906 T3904 arg1Of Belin,Dr.
R2849 T3906 T3905 arg1Of Belin,Dominique
R2850 T3908 T3909 arg1Of University,of
R2851 T3910 T3909 arg2Of Geneva.,of
R2852 T3911 T3908 arg1Of pDB9406,University
R2853 T3911 T3912 arg1Of pDB9406,contains
R2854 T3912 T3907 arg2Of contains,","
R2855 T3912 T3924 arg1Of contains,;
R2856 T3918 T3912 arg2Of fragment,contains
R2857 T3918 T3913 arg1Of fragment,a
R2858 T3918 T3914 arg1Of fragment,4.4
R2859 T3918 T3915 arg1Of fragment,kb
R2860 T3918 T3916 arg1Of fragment,EcoRI/SpeI
R2861 T3918 T3917 arg1Of fragment,genomic
R2862 T3918 T3919 arg1Of fragment,spanning
R2863 T3923 T3919 arg2Of site,spanning
R2864 T3923 T3920 arg1Of site,the
R2865 T3923 T3921 arg1Of site,transcription
R2866 T3923 T3922 arg1Of site,initiation
R2867 T3925 T3926 arg1Of pDB9402-41,and
R2868 T3926 T3928 arg1Of and,contain
R2869 T3927 T3926 arg2Of pDB9402-42,and
R2870 T3928 T3924 arg2Of contain,;
R2871 T3934 T3928 arg2Of fragment,contain
R2872 T3934 T3929 arg1Of fragment,a
R2873 T3934 T3930 arg1Of fragment,1.2
R2874 T3934 T3931 arg1Of fragment,kb
R2875 T3934 T3932 arg1Of fragment,EcoRI
R2876 T3934 T3933 arg1Of fragment,genomic
R2877 T3934 T3935 arg1Of fragment,","
R2878 T3934 T3936 arg1Of fragment,located
R2879 T3934 T3944 arg1Of fragment,","
R2880 T3934 T3945 arg2Of fragment,cloned
R2881 T3936 T3938 arg1Of located,upstream
R2882 T3938 T3937 arg1Of upstream,immediately
R2883 T3938 T3939 arg1Of upstream,of
R2884 T3943 T3939 arg2Of fragment,of
R2885 T3943 T3940 arg1Of fragment,the
R2886 T3943 T3941 arg1Of fragment,pDB9406
R2887 T3943 T3942 arg1Of fragment,EcoRI
R2888 T3945 T3946 arg1Of cloned,in
R2889 T3948 T3946 arg2Of orientations,in
R2890 T3948 T3947 arg1Of orientations,opposite
R2891 T3950 T3949 arg2Of inserts,Subcloned
R2892 T3950 T3951 arg1Of inserts,were
R2893 T3950 T3952 arg2Of inserts,verified
R2894 T3952 T3951 arg2Of verified,were
R2895 T3956 T3954 arg1Of digestion,restriction
R2896 T3956 T3955 arg1Of digestion,enzyme
R2897 T3956 T3957 arg1Of digestion,and
R2898 T3957 T3952 arg1Of and,verified
R2899 T3957 T3953 arg2Of and,by
R2900 T3959 T3957 arg2Of sequencing,and
R2901 T3959 T3958 arg1Of sequencing,DNA
R2902 T3962 T3960 arg1Of sequence,The
R2903 T3962 T3961 arg1Of sequence,nucleotide
R2904 T3962 T3963 arg1Of sequence,of
R2905 T3962 T3973 arg1Of sequence,was
R2906 T3962 T3974 arg2Of sequence,determined
R2907 T3972 T3963 arg2Of region,of
R2908 T3972 T3964 arg1Of region,the
R2909 T3972 T3965 arg1Of region,4480
R2910 T3972 T3966 arg1Of region,bp
R2911 T3972 T3967 arg1Of region,murine
R2912 T3972 T3968 arg1Of region,SerpinB2
R2913 T3972 T3969 arg1Of region,gene
R2914 T3972 T3970 arg1Of region,5′
R2915 T3972 T3971 arg1Of region,flanking
R2916 T3974 T3973 arg2Of determined,was
R2917 T3975 T3974 arg3Of using,determined
R2918 T3978 T3977 arg1Of PRISM,ABI
R2919 T3985 T3976 arg1Of kit,the
R2920 T3985 T3978 arg1Of kit,PRISM
R2921 T3985 T3979 arg1Of kit,dye
R2922 T3985 T3980 arg1Of kit,terminator
R2923 T3985 T3981 arg1Of kit,cycle
R2924 T3985 T3982 arg1Of kit,sequencing
R2925 T3985 T3983 arg1Of kit,ready
R2926 T3985 T3984 arg1Of kit,reaction
R2927 T3985 T3986 arg1Of kit,(
R2928 T3985 T3989 arg1Of kit,and
R2929 T3987 T3986 arg2Of Perkin-Elmer,(
R2930 T3988 T3986 arg3Of ),(
R2931 T3989 T3975 arg2Of and,using
R2932 T3993 T3989 arg2Of sequencer,and
R2933 T3993 T3990 arg1Of sequencer,a
R2934 T3993 T3991 arg1Of sequencer,PE
R2935 T3993 T3992 arg1Of sequencer,373A
R2936 T3993 T3994 arg1Of sequencer,(
R2937 T3995 T3994 arg2Of Perkin-Elmer,(
R2938 T3996 T3994 arg3Of ),(
R2939 T3998 T3997 arg1Of sequence,This
R2940 T3998 T3999 arg1Of sequence,was
R2941 T3998 T4000 arg2Of sequence,deposited
R2942 T4000 T3999 arg2Of deposited,was
R2943 T4000 T4001 arg1Of deposited,in
R2944 T4007 T4002 arg1Of No.,GenBank/EMBL/DDBJ
R2945 T4007 T4003 arg1Of No.,Data
R2946 T4007 T4004 arg1Of No.,Bank
R2947 T4007 T4005 arg1Of No.,with
R2948 T4007 T4006 arg1Of No.,Accession
R2949 T4008 T4001 arg2Of AF339731,in
R2950 T4008 T4007 arg1Of AF339731,No.
R3138 T4295 T4294 arg1Of Blot,Western
R3139 T4296 T4295 arg1Of Assays,Blot
R3140 T4299 T4297 arg1Of lysates,Whole
R3141 T4299 T4298 arg1Of lysates,cell
R3142 T4299 T4300 arg1Of lysates,were
R3143 T4299 T4301 arg2Of lysates,prepared
R3144 T4301 T4300 arg2Of prepared,were
R3145 T4301 T4302 arg1Of prepared,in
R3146 T4301 T4305 arg1Of prepared,(
R3147 T4304 T4302 arg2Of buffer,in
R3148 T4304 T4303 arg1Of buffer,RIPA
R3149 T4308 T4305 arg2Of Tris,(
R3150 T4308 T4306 arg1Of Tris,10
R3151 T4308 T4307 arg1Of Tris,mM
R3152 T4308 T4309 arg1Of Tris,","
R3153 T4308 T4326 arg1Of Tris,","
R3154 T4310 T4311 arg1Of 150,mM
R3155 T4312 T4310 arg1Of NaCl,150
R3156 T4312 T4313 arg1Of NaCl,","
R3157 T4313 T4318 arg1Of ",",","
R3158 T4314 T4315 arg1Of 1,%
R3159 T4317 T4313 arg2Of X-100,","
R3160 T4317 T4314 arg1Of X-100,1
R3161 T4317 T4316 arg1Of X-100,Triton
R3162 T4318 T4322 arg1Of ",",","
R3163 T4319 T4320 arg1Of 0.5,%
R3164 T4321 T4318 arg2Of NP-40,","
R3165 T4321 T4319 arg1Of NP-40,0.5
R3166 T4322 T4309 arg2Of ",",","
R3167 T4323 T4324 arg1Of 0.5,%
R3168 T4325 T4322 arg2Of deoxycholate,","
R3169 T4325 T4323 arg1Of deoxycholate,0.5
R3170 T4327 T4328 arg1Of 0.1,%
R3171 T4329 T4326 arg2Of SDS,","
R3172 T4329 T4327 arg1Of SDS,0.1
R3173 T4330 T4305 arg3Of ),(
R3174 T4332 T4333 arg1Of proteins,were
R3175 T4332 T4334 arg2Of proteins,separated
R3177 T4334 T4335 arg1Of separated,on
R3179 T4336 T4337 arg1Of 4–12,%
R3180 T4340 T4335 arg2Of gels,on
R3181 T4340 T4336 arg1Of gels,4–12
R3182 T4340 T4338 arg1Of gels,Bis-Tris
R3183 T4340 T4339 arg1Of gels,NuPAGE
R3184 T4340 T4341 arg1Of gels,(
R3185 T4342 T4341 arg2Of Invitrogen,(
R3186 T4343 T4341 arg3Of ),(
R2622 T3606 T3604 arg1Of PCR,Real-time
R2623 T3606 T3605 arg1Of PCR,Quantitative
R2624 T3606 T3607 arg1Of PCR,(
R2625 T3608 T3607 arg2Of qPCR,(
R2626 T3609 T3607 arg3Of ),(
R2627 T3613 T3610 arg1Of Kit,The
R2628 T3613 T3611 arg1Of Kit,RNeasy
R2629 T3613 T3612 arg1Of Kit,Mini
R2631 T3613 T3617 arg1Of Kit,was
R2635 T3616 T3614 arg3Of ),(
R2636 T3618 T3617 arg2Of used,was
R2802 T3860 T3859 arg2Of addition,in

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T3590 1151-1158 Protein denotes β-actin
T3589 1125-1130 Protein denotes Cebpb
T3588 1099-1107 Protein denotes SerpinB2
T3587 1090-1098 Protein denotes Serpinb2
T3825 2091-2099 Protein denotes SerpinB2
T3824 1456-1471 Protein denotes exonuclease III
T3823 1251-1259 Protein denotes SerpinB2

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T4013 2091-2099 http://www.uniprot.org/uniprot/P05120 denotes SerpinB2
T4012 1251-1259 http://www.uniprot.org/uniprot/P05120 denotes SerpinB2
T3680 1151-1158 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3679 1125-1130 http://www.uniprot.org/uniprot/P28033 denotes Cebpb

UBERON-AE

Id Subject Object Predicate Lexical cue
T3158 442-447 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T3157 41-46 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-BP

Id Subject Object Predicate Lexical cue
T3828 1770-1783 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T3827 2015-2024 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T3826 1472-1481 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T3594 1058-1073 http://purl.obolibrary.org/obo/GO_0010467 denotes Gene Expression
T3593 980-993 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T3592 972-993 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse Transcription
T3591 905-914 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis

GO-CC

Id Subject Object Predicate Lexical cue
T4277 2380-2384 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3595 889-894 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3173 449-453 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell

sentences

Id Subject Object Predicate Lexical cue
T4260 2354-2373 Sentence denotes Western Blot Assays
T3816 2265-2352 Sentence denotes This sequence was deposited in GenBank/EMBL/DDBJ Data Bank with Accession No. AF339731.
T3815 2045-2264 Sentence denotes The nucleotide sequence of the 4480 bp murine SerpinB2 gene 5′ flanking region was determined using the ABI PRISM dye terminator cycle sequencing ready reaction kit (Perkin-Elmer) and a PE 373A sequencer (Perkin-Elmer).
T3814 1961-2044 Sentence denotes Subcloned inserts were verified by restriction enzyme digestion and DNA sequencing.
T3813 1483-1960 Sentence denotes Plasmids pDB9406, pDB9402-41 and pDB9402-42 containing genomic DNA isolated from a λFIXII (Stratagene) genomic library prepared from a 129 mouse strain, were kindly provided by Dr. Dominique Belin, University of Geneva. pDB9406 contains a 4.4 kb EcoRI/SpeI genomic fragment spanning the transcription initiation site; pDB9402-41 and pDB9402-42 contain a 1.2 kb EcoRI genomic fragment, located immediately upstream of the pDB9406 EcoRI fragment, cloned in opposite orientations.
T3812 1220-1482 Sentence denotes The DNA sequence of the murine SerpinB2 promoter was determined by sequencing the pUC-based plasmids pDB9406, pDB9402-41 and pDB9402-42, in addition to plasmids prepared from pDB9402-41 and pDB9402-42 containing deletions introduced by exonuclease III digestion.
T3811 1198-1219 Sentence denotes DNA Sequence Analysis
T3582 896-1196 Sentence denotes For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems).
T3581 825-895 Sentence denotes The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells.
T3580 791-824 Sentence denotes Real-time Quantitative PCR (qPCR)
T3165 694-789 Sentence denotes Bacterial lipopolysaccharide (LPS) (Salmonella minnesota Strain Re595) was obtained from Sigma.
T3164 531-693 Sentence denotes All cultures were routinely checked to exclude Mycoplasma infection by nuclear staining using Hoechst stain 33258 (Sigma) and the MycoAlert Detection Kit (Lonza).
T3163 449-530 Sentence denotes Cell viability was determined using the trypan blue (Sigma) dye exclusion method.
T3162 304-448 Sentence denotes Precautions were taken to exclude bacterial lipopolysaccharide contamination from all cell cultures through the use of certified LPS-free serum.
T3161 108-303 Sentence denotes Primary peritoneal macrophages were obtained by injecting C57BL/6 mice with 3% thioglycollate broth followed by peritoneal lavage 3–5 days later, and maintained in 50% DMEM/F12 media (Gibco BRL).
T37 108-303 Sentence denotes Primary peritoneal macrophages were obtained by injecting C57BL/6 mice with 3% thioglycollate broth followed by peritoneal lavage 3–5 days later, and maintained in 50% DMEM/F12 media (Gibco BRL).
T38 304-448 Sentence denotes Precautions were taken to exclude bacterial lipopolysaccharide contamination from all cell cultures through the use of certified LPS-free serum.
T39 449-530 Sentence denotes Cell viability was determined using the trypan blue (Sigma) dye exclusion method.
T40 531-693 Sentence denotes All cultures were routinely checked to exclude Mycoplasma infection by nuclear staining using Hoechst stain 33258 (Sigma) and the MycoAlert Detection Kit (Lonza).
T41 694-789 Sentence denotes Bacterial lipopolysaccharide (LPS) (Salmonella minnesota Strain Re595) was obtained from Sigma.
T42 791-824 Sentence denotes Real-time Quantitative PCR (qPCR)
T43 825-895 Sentence denotes The RNeasy Mini Kit (Qiagen) was used to isolate total RNA from cells.
T44 896-1196 Sentence denotes For cDNA synthesis, 1 µg of total RNA was reverse transcribed using TaqMan® Reverse Transcription Reagents (Applied Biosystems). qPCR was performed using TaqMan® Gene Expression 20X primers for Serpinb2/SerpinB2 (Mm00440905_m1), Cebpb (Mm00843434_s1) and β-actin (Mm00607939_s1) (Applied Biosystems).
T45 1198-1219 Sentence denotes DNA Sequence Analysis
T46 1220-1482 Sentence denotes The DNA sequence of the murine SerpinB2 promoter was determined by sequencing the pUC-based plasmids pDB9406, pDB9402-41 and pDB9402-42, in addition to plasmids prepared from pDB9402-41 and pDB9402-42 containing deletions introduced by exonuclease III digestion.
T47 1483-1960 Sentence denotes Plasmids pDB9406, pDB9402-41 and pDB9402-42 containing genomic DNA isolated from a λFIXII (Stratagene) genomic library prepared from a 129 mouse strain, were kindly provided by Dr. Dominique Belin, University of Geneva. pDB9406 contains a 4.4 kb EcoRI/SpeI genomic fragment spanning the transcription initiation site; pDB9402-41 and pDB9402-42 contain a 1.2 kb EcoRI genomic fragment, located immediately upstream of the pDB9406 EcoRI fragment, cloned in opposite orientations.
T48 1961-2044 Sentence denotes Subcloned inserts were verified by restriction enzyme digestion and DNA sequencing.
T49 2045-2264 Sentence denotes The nucleotide sequence of the 4480 bp murine SerpinB2 gene 5′ flanking region was determined using the ABI PRISM dye terminator cycle sequencing ready reaction kit (Perkin-Elmer) and a PE 373A sequencer (Perkin-Elmer).
T50 2265-2342 Sentence denotes This sequence was deposited in GenBank/EMBL/DDBJ Data Bank with Accession No.
T51 2343-2352 Sentence denotes AF339731.
T52 2354-2373 Sentence denotes Western Blot Assays

ICD10

Id Subject Object Predicate Lexical cue
T3171 578-598 http://purl.bioontology.org/ontology/ICD10/A49.3 denotes Mycoplasma infection

simple1

Id Subject Object Predicate Lexical cue
T3603 1151-1158 Protein denotes β-actin
T3602 1125-1130 Protein denotes Cebpb
T3601 1099-1107 Protein denotes SerpinB2
T3600 1090-1098 Protein denotes Serpinb2
T3834 2091-2099 Protein denotes SerpinB2
T3833 1456-1471 Protein denotes exonuclease III
T3832 1251-1259 Protein denotes SerpinB2

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T4011 2091-2099 Protein denotes SerpinB2
T4010 1456-1471 Protein denotes exonuclease III
T4009 1251-1259 Protein denotes SerpinB2
T3678 1063-1073 Gene_expression denotes Expression
T3677 1151-1158 Protein denotes β-actin
T3676 1125-1130 Protein denotes Cebpb
T3675 1099-1107 Protein denotes SerpinB2
T3674 1090-1098 Protein denotes Serpinb2
R2694 T3674 T3678 themeOf Serpinb2,Expression
R2695 T3675 T3678 themeOf SerpinB2,Expression
R2696 T3676 T3678 themeOf Cebpb,Expression
R2697 T3677 T3678 themeOf β-actin,Expression

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T4022 2091-2099 Protein denotes SerpinB2
T4021 1456-1471 Protein denotes exonuclease III
T4020 1251-1259 Protein denotes SerpinB2
T3692 1151-1158 Protein denotes β-actin
T3691 1125-1130 Protein denotes Cebpb
T3690 1099-1107 Protein denotes SerpinB2
T3689 1090-1098 Protein denotes Serpinb2

DLUT931

Id Subject Object Predicate Lexical cue
T4016 2091-2099 Protein denotes SerpinB2
T4015 1456-1471 Protein denotes exonuclease III
T4014 1251-1259 Protein denotes SerpinB2
T3684 1151-1158 Protein denotes β-actin
T3683 1125-1130 Protein denotes Cebpb
T3682 1099-1107 Protein denotes SerpinB2
T3681 1090-1098 Protein denotes Serpinb2

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4499 2435-2446 Entity denotes 150 mM NaCl
T4497 2520-2547 Entity denotes separated on 4–12% Bis-Tris
T4495 2495-2503 Protein denotes 0.1% SDS
T4493 2476-2504 Entity denotes 0.5% deoxycholate, 0.1% SDS)
T4490 2423-2468 Entity denotes 10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5%
T4485 2429-2447 Entity denotes Tris, 150 mM NaCl,
T4482 2555-2572 Entity denotes gels (Invitrogen)
T4481 2506-2514 Protein denotes proteins
T4057 2091-2099 Protein denotes SerpinB2
T4056 1198-1201 Entity denotes DNA
T4055 2296-2338 Protein denotes GenBank/EMBL/DDBJ Data Bank with Accession
T4054 1538-1634 Entity denotes genomic DNA isolated from a λFIXII (Stratagene) genomic library prepared from a 129 mouse strain
T4053 1912-1927 Entity denotes EcoRI fragment,
T4052 2299-2303 Protein denotes Bank
T4051 2049-2059 Entity denotes nucleotide
T4050 2045-2107 Protein denotes The nucleotide sequence of the 4480 bp murine SerpinB2 gene 5′
T4049 1321-1324 Entity denotes pDB
T4048 1224-1227 Entity denotes DNA
T4047 1996-2024 Protein denotes restriction enzyme digestion
T4046 2145-2158 Protein denotes the ABI PRISM
T4045 2296-2338 Protein denotes GenBank/EMBL/DDBJ Data Bank with Accession
T4044 1251-1259 Protein denotes SerpinB2
T4043 1766-1799 Entity denotes the transcription initiation site
T4042 2229-2233 Protein denotes a PE
T4041 1844-1847 Protein denotes Eco
T4040 1729-1734 Entity denotes EcoRI
T4039 1237-1268 Entity denotes of the murine SerpinB2 promoter
T4038 1298-1303 Entity denotes the p
T4037 1456-1481 Protein denotes exonuclease III digestion
T3719 1050-1085 Gene_expression denotes TaqMan® Gene Expression 20X primers
T3718 884-894 Entity denotes from cells
T3717 924-933 Protein denotes total RNA
T3716 874-883 Protein denotes total RNA
T3715 819-823 Protein denotes qPCR
T3714 791-824 Protein denotes Real-time Quantitative PCR (qPCR)
T3713 1125-1146 Protein denotes Cebpb (Mm00843434_s1)
T3712 836-853 Protein denotes Mini Kit (Qiagen)
T3711 1151-1174 Protein denotes β-actin (Mm00607939_s1)
T3710 1050-1062 Protein denotes TaqMan® Gene
T3709 964-1023 Entity denotes TaqMan® Reverse Transcription Reagents (Applied Biosystems)
T3708 900-914 Entity denotes cDNA synthesis
T3707 1090-1124 Protein denotes Serpinb2/SerpinB2 (Mm00440905_m1),
T3315 108-237 Binding denotes Primary peritoneal macrophages were obtained by injecting C57BL/6 mice with 3% thioglycollate broth followed by peritoneal lavage
T3314 570-618 Regulation denotes exclude Mycoplasma infection by nuclear staining
T3313 54-64 Entity denotes penicillin
T3312 449-463 Entity denotes Cell viability
T3311 502-507 Protein denotes Sigma
T3310 108-138 Entity denotes Primary peritoneal macrophages
T3309 489-508 Entity denotes trypan blue (Sigma)
T3308 65-77 Entity denotes streptomycin
T3307 694-728 Entity denotes Bacterial lipopolysaccharide (LPS)
T3306 433-441 Entity denotes LPS-free
T3305 281-284 Protein denotes F12
T3304 292-301 Protein denotes Gibco BRL
T3303 54-87 Entity denotes penicillin/streptomycin/glutamate
T3302 724-727 Entity denotes LPS
T3301 187-201 Entity denotes thioglycollate
T3300 671-692 Protein denotes Detection Kit (Lonza)
T3299 348-366 Entity denotes lipopolysaccharide
T3298 783-788 Protein denotes Sigma
T3297 54-87 Entity denotes penicillin/streptomycin/glutamate
T3296 646-651 Protein denotes Sigma
R2698 T3710 T3719 themeOf TaqMan® Gene,TaqMan® Gene Expression 20X primers
R2951 T4039 T4044 partOf of the murine SerpinB2 promoter,SerpinB2

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T4029 2149-2158 Protein denotes ABI PRISM
T4028 1904-1926 Protein denotes pDB9406 EcoRI fragment
T4027 1844-1849 Protein denotes EcoRI
T4026 1735-1739 Protein denotes SpeI
T4025 1729-1734 Protein denotes EcoRI
T4024 1456-1471 Protein denotes exonuclease III
T4023 1244-1268 Protein denotes murine SerpinB2 promoter
T3699 1160-1173 Protein denotes Mm00607939_s1
T3698 1151-1158 Protein denotes β-actin
T3697 1132-1145 Protein denotes Mm00843434_s1
T3696 1125-1130 Protein denotes Cebpb
T3695 1109-1122 Protein denotes Mm00440905_m1
T3694 1099-1107 Protein denotes SerpinB2
T3693 1090-1098 Protein denotes Serpinb2

testone

Id Subject Object Predicate Lexical cue
T3803 1456-1471 Protein denotes exonuclease III
T3802 1251-1259 Protein denotes SerpinB2
T3804 2091-2099 Protein denotes SerpinB2
T3571 1151-1158 Protein denotes β-actin
T3570 1125-1130 Protein denotes Cebpb
T3569 1099-1107 Protein denotes SerpinB2
T3568 1090-1098 Protein denotes Serpinb2

test3

Id Subject Object Predicate Lexical cue
T3810 2091-2099 Protein denotes SerpinB2
T3809 1456-1471 Protein denotes exonuclease III
T3808 1251-1259 Protein denotes SerpinB2
T3807 2091-2099 Protein denotes SerpinB2
T3806 1456-1471 Protein denotes exonuclease III
T3805 1251-1259 Protein denotes SerpinB2
T3579 1151-1158 Protein denotes β-actin
T3578 1125-1130 Protein denotes Cebpb
T3577 1099-1107 Protein denotes SerpinB2
T3576 1090-1098 Protein denotes Serpinb2
T3575 1151-1158 Protein denotes β-actin
T3574 1125-1130 Protein denotes Cebpb
T3573 1099-1107 Protein denotes SerpinB2
T3572 1090-1098 Protein denotes Serpinb2