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Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T26146","span":{"begin":495,"end":502},"obj":"Protein"},{"id":"T26145","span":{"begin":465,"end":480},"obj":"Protein"},{"id":"T26144","span":{"begin":434,"end":444},"obj":"Protein"},{"id":"T26143","span":{"begin":395,"end":419},"obj":"Protein"},{"id":"T26142","span":{"begin":353,"end":390},"obj":"Protein"},{"id":"T26141","span":{"begin":223,"end":260},"obj":"Protein"},{"id":"T26140","span":{"begin":184,"end":189},"obj":"Protein"},{"id":"T26139","span":{"begin":171,"end":176},"obj":"Protein"},{"id":"T26138","span":{"begin":127,"end":135},"obj":"Protein"},{"id":"T26137","span":{"begin":105,"end":110},"obj":"Protein"},{"id":"T26136","span":{"begin":80,"end":85},"obj":"Protein"},{"id":"T26135","span":{"begin":8,"end":16},"obj":"Protein"},{"id":"T26152","span":{"begin":841,"end":878},"obj":"Protein"},{"id":"T26151","span":{"begin":782,"end":814},"obj":"Protein"},{"id":"T26150","span":{"begin":757,"end":764},"obj":"Protein"},{"id":"T26149","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T26148","span":{"begin":651,"end":659},"obj":"Protein"},{"id":"T26147","span":{"begin":594,"end":601},"obj":"Protein"},{"id":"T26158","span":{"begin":1226,"end":1233},"obj":"Protein"},{"id":"T26157","span":{"begin":1148,"end":1160},"obj":"Protein"},{"id":"T26156","span":{"begin":1110,"end":1142},"obj":"Protein"},{"id":"T26155","span":{"begin":1006,"end":1021},"obj":"Protein"},{"id":"T26154","span":{"begin":953,"end":963},"obj":"Protein"},{"id":"T26153","span":{"begin":883,"end":907},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T26512","span":{"begin":953,"end":963},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26511","span":{"begin":859,"end":869},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26510","span":{"begin":434,"end":444},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26509","span":{"begin":371,"end":381},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26508","span":{"begin":241,"end":251},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T26507","span":{"begin":841,"end":849},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26506","span":{"begin":651,"end":659},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26505","span":{"begin":353,"end":361},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26504","span":{"begin":223,"end":231},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26503","span":{"begin":127,"end":135},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26502","span":{"begin":8,"end":16},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T26500","span":{"begin":679,"end":684},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26499","span":{"begin":184,"end":189},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26498","span":{"begin":171,"end":176},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26497","span":{"begin":105,"end":110},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26496","span":{"begin":80,"end":85},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26495","span":{"begin":1110,"end":1117},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26494","span":{"begin":782,"end":789},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26493","span":{"begin":757,"end":764},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26492","span":{"begin":594,"end":601},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T26491","span":{"begin":495,"end":502},"obj":"http://www.uniprot.org/uniprot/P28033"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}

{"project":"GO-BP","denotations":[{"id":"T26178","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0050397"},{"id":"T26177","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T26176","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T26175","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T26174","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T26173","span":{"begin":575,"end":590},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T26172","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26171","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26170","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26169","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26168","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26167","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26166","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_1903770"},{"id":"T26165","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_1903770"},{"id":"T26164","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_1903771"},{"id":"T26163","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_1903771"},{"id":"T26162","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T26161","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T26160","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T26159","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0004565"}],"text":"(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}

{"project":"GO-MF","denotations":[{"id":"T26193","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0050397"},{"id":"T26192","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T26191","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T26190","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T26189","span":{"begin":953,"end":972},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T26188","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26187","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0033930"},{"id":"T26181","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0004336"},{"id":"T26180","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T26179","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0004565"},{"id":"T26186","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26185","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0033929"},{"id":"T26184","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26183","span":{"begin":465,"end":489},"obj":"http://purl.obolibrary.org/obo/GO_0033920"},{"id":"T26182","span":{"begin":1006,"end":1030},"obj":"http://purl.obolibrary.org/obo/GO_0004336"}],"text":"(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}

{"project":"sentences","denotations":[{"id":"T26094","span":{"begin":1235,"end":1368},"obj":"Sentence"},{"id":"T26093","span":{"begin":1144,"end":1234},"obj":"Sentence"},{"id":"T26092","span":{"begin":1032,"end":1143},"obj":"Sentence"},{"id":"T26091","span":{"begin":953,"end":1031},"obj":"Sentence"},{"id":"T26090","span":{"begin":679,"end":952},"obj":"Sentence"},{"id":"T26089","span":{"begin":308,"end":678},"obj":"Sentence"},{"id":"T26088","span":{"begin":120,"end":307},"obj":"Sentence"},{"id":"T26087","span":{"begin":0,"end":119},"obj":"Sentence"},{"id":"T224","span":{"begin":0,"end":119},"obj":"Sentence"},{"id":"T225","span":{"begin":120,"end":307},"obj":"Sentence"},{"id":"T226","span":{"begin":308,"end":678},"obj":"Sentence"},{"id":"T227","span":{"begin":679,"end":952},"obj":"Sentence"},{"id":"T228","span":{"begin":953,"end":1031},"obj":"Sentence"},{"id":"T229","span":{"begin":1032,"end":1143},"obj":"Sentence"},{"id":"T230","span":{"begin":1144,"end":1234},"obj":"Sentence"},{"id":"T231","span":{"begin":1235,"end":1368},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"(A) The SerpinB2 proximal promoter is significantly activated in LPS-stimulated Cebpb+/+ MEFs and not in Cebpb−/− MEFs. Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}

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Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}

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Murine SerpinB2 gene promoter activity measured in Cebpb+/+ and Cebpb−/− MEFs expressing the pGLmP-539 SerpinB2 promoter-luciferase reporter in the presence or absence of LPS (100 ng/mL). Cells were co-transfected with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and luciferase units normalized to β-galactosidase activity. (B) C/EBP-β gene schematic depicting gene structure and phospho-acceptor sites. (C) Phosphorylation of C/EBP-β at Serine 64 negatively regulates LPS-stimulated SerpinB2 promoter activity. Cebpb−/− MEFs were co-transfected with expression plasmids encoding wild-type C/EBP-β or the indicated C/EBP-β phospho-acceptor mutants, along with the pGLmP-539 SerpinB2 promoter-luciferase reporter and β-galactosidase reporter plasmids, and stimulated with LPS for 4 hrs. Luciferase activity was quantified and normalized to β-galactosidase activity. The western blot (below the graphs) confirms the expression of the respective C/EBP-β phospho-acceptor mutants. The C/EBP-βT217A -transfected MEFs express both the 38 kDa and 35 kDa isoforms of C/EBP-β. The results represent the mean and SEM of at least three independent experiments performed in triplicate. (*, p\u003c0.05, one-way ANOVA).\n"}